Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036757.t1 | RHN63957.1 | 96.6 | 117 | 4 | 0 | 1 | 117 | 75 | 191 | 2.90E-56 | 227.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036757.t1 | Q8GW46 | 76.5 | 98 | 18 | 1 | 3 | 100 | 71 | 163 | 5.4e-35 | 148.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036757.t1 | A0A396IM32 | 96.6 | 117 | 4 | 0 | 1 | 117 | 75 | 191 | 2.1e-56 | 227.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene036757.t1 | TF | C2C2-YABBY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036757.t1 | MTR_4g114730 | 95.726 | 117 | 5 | 0 | 1 | 117 | 75 | 191 | 4.29e-81 | 236 |
| MS.gene036757.t1 | MTR_2g087740 | 86.154 | 65 | 9 | 0 | 37 | 101 | 117 | 181 | 7.18e-38 | 126 |
| MS.gene036757.t1 | MTR_4g050300 | 78.462 | 65 | 9 | 1 | 37 | 101 | 107 | 166 | 9.04e-31 | 108 |
| MS.gene036757.t1 | MTR_4g025900 | 63.736 | 91 | 31 | 1 | 3 | 91 | 96 | 186 | 1.38e-30 | 108 |
| MS.gene036757.t1 | MTR_4g101660 | 88.462 | 52 | 6 | 0 | 40 | 91 | 130 | 181 | 4.01e-29 | 105 |
| MS.gene036757.t1 | MTR_5g034030 | 78.431 | 51 | 11 | 0 | 34 | 84 | 112 | 162 | 1.53e-24 | 93.2 |
| MS.gene036757.t1 | MTR_5g046230 | 71.429 | 49 | 14 | 0 | 35 | 83 | 107 | 155 | 6.37e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036757.t1 | AT2G26580 | 76.531 | 98 | 18 | 1 | 3 | 100 | 71 | 163 | 7.07e-49 | 153 |
| MS.gene036757.t1 | AT2G26580 | 76.531 | 98 | 18 | 1 | 3 | 100 | 71 | 163 | 7.07e-49 | 153 |
| MS.gene036757.t1 | AT1G08465 | 85.714 | 63 | 9 | 0 | 37 | 99 | 107 | 169 | 7.32e-36 | 121 |
| MS.gene036757.t1 | AT2G45190 | 92.982 | 57 | 4 | 0 | 35 | 91 | 70 | 126 | 1.00e-34 | 117 |
| MS.gene036757.t1 | AT2G45190 | 92.982 | 57 | 4 | 0 | 35 | 91 | 134 | 190 | 3.07e-34 | 118 |
| MS.gene036757.t1 | AT4G00180 | 90.741 | 54 | 5 | 0 | 40 | 93 | 120 | 173 | 2.41e-31 | 110 |
| MS.gene036757.t1 | AT4G00180 | 90.741 | 54 | 5 | 0 | 40 | 93 | 151 | 204 | 4.84e-31 | 110 |
| MS.gene036757.t1 | AT1G23420 | 74.545 | 55 | 14 | 0 | 33 | 87 | 126 | 180 | 1.54e-24 | 93.6 |
| MS.gene036757.t1 | AT1G23420 | 74.545 | 55 | 14 | 0 | 33 | 87 | 157 | 211 | 3.50e-24 | 93.2 |
| MS.gene036757.t1 | AT1G69180 | 69.388 | 49 | 15 | 0 | 35 | 83 | 108 | 156 | 4.04e-20 | 80.9 |
Find 23 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATCCTATCGTTGCATT+TGG | 0.296610 | 8.1:-11645194 | None:intergenic |
| GCTTCTCTGTGACTGATATC+AGG | 0.363234 | 8.1:-11645704 | None:intergenic |
| GCAATCCTATCGTTGCATTT+GGG | 0.399881 | 8.1:-11645193 | None:intergenic |
| AGGTACACGTTGCCTCTTCT+CGG | 0.458670 | 8.1:-11645515 | None:intergenic |
| ATCAGCCCAAAATGAATATG+AGG | 0.476928 | 8.1:-11645845 | None:intergenic |
| CACCCATGTTACTGAGGAAA+GGG | 0.483814 | 8.1:+11645234 | MS.gene036757:CDS |
| GAGCAACAATCAAGCTAAGA+TGG | 0.499438 | 8.1:+11645874 | MS.gene036757:CDS |
| TCCTCAGTAACATGGGTGGT+TGG | 0.501150 | 8.1:-11645229 | None:intergenic |
| ACTGCATGAATCCAGAGTAT+AGG | 0.501971 | 8.1:+11645152 | None:intergenic |
| GGTACACGTTGCCTCTTCTC+GGG | 0.503039 | 8.1:-11645514 | None:intergenic |
| CAACCCTTTCCTCAGTAACA+TGG | 0.504380 | 8.1:-11645237 | None:intergenic |
| TGAGAAGCGTTTGATGTCTA+CGG | 0.515736 | 8.1:+11646005 | MS.gene036757:CDS |
| CCTATCGTTGCATTTGGGAG+TGG | 0.533061 | 8.1:-11645188 | None:intergenic |
| AATTTCCAGCTCCCGAGAAG+AGG | 0.540719 | 8.1:+11645503 | MS.gene036757:intron |
| CTAGTTCGAATCCTATACTC+TGG | 0.567724 | 8.1:-11645163 | None:intergenic |
| GGAAGAGATTCAGAGGATCA+AGG | 0.576711 | 8.1:+11645673 | MS.gene036757:CDS |
| CCACCCATGTTACTGAGGAA+AGG | 0.581614 | 8.1:+11645233 | MS.gene036757:CDS |
| ATGAACTGATTATAAGCAGA+AGG | 0.586762 | 8.1:-11645535 | None:intergenic |
| CCACTCCCAAATGCAACGAT+AGG | 0.592244 | 8.1:+11645188 | MS.gene036757:CDS |
| TATGCAGGGAAGAGATTCAG+AGG | 0.621557 | 8.1:+11645666 | MS.gene036757:intron |
| AACCCTTTCCTCAGTAACAT+GGG | 0.626838 | 8.1:-11645236 | None:intergenic |
| ACCAACCACCCATGTTACTG+AGG | 0.669098 | 8.1:+11645228 | MS.gene036757:CDS |
| CCTTTCCTCAGTAACATGGG+TGG | 0.674444 | 8.1:-11645233 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGATTGAAAAATTAAAT+TGG | - | chr8.1:11645922-11645941 | None:intergenic | 10.0% |
| !!! | ATTATTTCTTTATTTTTTAG+TGG | + | chr8.1:11645813-11645832 | MS.gene036757:intron | 10.0% |
| !!! | TGATTATTTTTATTATATTC+AGG | + | chr8.1:11645978-11645997 | MS.gene036757:intron | 10.0% |
| !!! | TTATTTCTTTATTTTTTAGT+GGG | + | chr8.1:11645814-11645833 | MS.gene036757:intron | 10.0% |
| !! | TTTAAATAGATAGGAAAAAA+AGG | - | chr8.1:11645299-11645318 | None:intergenic | 15.0% |
| !!! | CATAATTTTATATATATGCA+GGG | + | chr8.1:11645652-11645671 | MS.gene036757:intron | 15.0% |
| !!! | TCATAATTTTATATATATGC+AGG | + | chr8.1:11645651-11645670 | MS.gene036757:intron | 15.0% |
| !! | AATTTAGAAAATAAACTACG+TGG | - | chr8.1:11645401-11645420 | None:intergenic | 20.0% |
| !!! | ATTTTCCTCATATTCATTTT+GGG | + | chr8.1:11645840-11645859 | MS.gene036757:CDS | 20.0% |
| !!! | GTATATATAGTTGATTCAAT+TGG | + | chr8.1:11645597-11645616 | MS.gene036757:intron | 20.0% |
| !! | CATTTTCCTCATATTCATTT+TGG | + | chr8.1:11645839-11645858 | MS.gene036757:CDS | 25.0% |
| !! | TAGTATTTCTATGTTCGTTA+AGG | - | chr8.1:11645433-11645452 | None:intergenic | 25.0% |
| ATGAACTGATTATAAGCAGA+AGG | - | chr8.1:11645538-11645557 | None:intergenic | 30.0% | |
| ! | GAGAGTATATAGTAAACTCA+CGG | - | chr8.1:11645270-11645289 | None:intergenic | 30.0% |
| AGTATATAGTAAACTCACGG+CGG | - | chr8.1:11645267-11645286 | None:intergenic | 35.0% | |
| ATCAGCCCAAAATGAATATG+AGG | - | chr8.1:11645848-11645867 | None:intergenic | 35.0% | |
| TAAACTACGTGGCTATGATA+TGG | - | chr8.1:11645390-11645409 | None:intergenic | 35.0% | |
| ! | TATTCATTTTGGGCTGATGT+TGG | + | chr8.1:11645850-11645869 | MS.gene036757:CDS | 35.0% |
| AACCCTTTCCTCAGTAACAT+GGG | - | chr8.1:11645239-11645258 | None:intergenic | 40.0% | |
| CTAGTTCGAATCCTATACTC+TGG | - | chr8.1:11645166-11645185 | None:intergenic | 40.0% | |
| GAGCAACAATCAAGCTAAGA+TGG | + | chr8.1:11645874-11645893 | MS.gene036757:CDS | 40.0% | |
| GCAATCCTATCGTTGCATTT+GGG | - | chr8.1:11645196-11645215 | None:intergenic | 40.0% | |
| TGCAATCCTATCGTTGCATT+TGG | - | chr8.1:11645197-11645216 | None:intergenic | 40.0% | |
| !! | TGAGAAGCGTTTGATGTCTA+CGG | + | chr8.1:11646005-11646024 | MS.gene036757:CDS | 40.0% |
| CAACCCTTTCCTCAGTAACA+TGG | - | chr8.1:11645240-11645259 | None:intergenic | 45.0% | |
| CACCCATGTTACTGAGGAAA+GGG | + | chr8.1:11645234-11645253 | MS.gene036757:CDS | 45.0% | |
| GCTTCTCTGTGACTGATATC+AGG | - | chr8.1:11645707-11645726 | None:intergenic | 45.0% | |
| GGAAGAGATTCAGAGGATCA+AGG | + | chr8.1:11645673-11645692 | MS.gene036757:CDS | 45.0% | |
| TATGCAGGGAAGAGATTCAG+AGG | + | chr8.1:11645666-11645685 | MS.gene036757:intron | 45.0% | |
| !! | TTTAAATATAAAATAACTAA+TGG | + | chr8.1:11645313-11645332 | MS.gene036757:intron | 5.0% |
| !!! | TATTTTATATTTAAATAGAT+AGG | - | chr8.1:11645308-11645327 | None:intergenic | 5.0% |
| AATTTCCAGCTCCCGAGAAG+AGG | + | chr8.1:11645503-11645522 | MS.gene036757:intron | 50.0% | |
| ACCAACCACCCATGTTACTG+AGG | + | chr8.1:11645228-11645247 | MS.gene036757:CDS | 50.0% | |
| AGGTACACGTTGCCTCTTCT+CGG | - | chr8.1:11645518-11645537 | None:intergenic | 50.0% | |
| CCACCCATGTTACTGAGGAA+AGG | + | chr8.1:11645233-11645252 | MS.gene036757:CDS | 50.0% | |
| CCACTCCCAAATGCAACGAT+AGG | + | chr8.1:11645188-11645207 | MS.gene036757:CDS | 50.0% | |
| CCTATCGTTGCATTTGGGAG+TGG | - | chr8.1:11645191-11645210 | None:intergenic | 50.0% | |
| CCTTTCCTCAGTAACATGGG+TGG | - | chr8.1:11645236-11645255 | None:intergenic | 50.0% | |
| TCCTCAGTAACATGGGTGGT+TGG | - | chr8.1:11645232-11645251 | None:intergenic | 50.0% | |
| GGTACACGTTGCCTCTTCTC+GGG | - | chr8.1:11645517-11645536 | None:intergenic | 55.0% | |
| CGTTGCCTCTTCTCGGGAGC+TGG | - | chr8.1:11645511-11645530 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 11645157 | 11646047 | 11645157 | ID=MS.gene036757 |
| chr8.1 | mRNA | 11645157 | 11646047 | 11645157 | ID=MS.gene036757.t1;Parent=MS.gene036757 |
| chr8.1 | exon | 11645157 | 11645268 | 11645157 | ID=MS.gene036757.t1.exon1;Parent=MS.gene036757.t1 |
| chr8.1 | CDS | 11645157 | 11645268 | 11645157 | ID=cds.MS.gene036757.t1;Parent=MS.gene036757.t1 |
| chr8.1 | exon | 11645512 | 11645560 | 11645512 | ID=MS.gene036757.t1.exon2;Parent=MS.gene036757.t1 |
| chr8.1 | CDS | 11645512 | 11645560 | 11645512 | ID=cds.MS.gene036757.t1;Parent=MS.gene036757.t1 |
| chr8.1 | exon | 11645673 | 11645748 | 11645673 | ID=MS.gene036757.t1.exon3;Parent=MS.gene036757.t1 |
| chr8.1 | CDS | 11645673 | 11645748 | 11645673 | ID=cds.MS.gene036757.t1;Parent=MS.gene036757.t1 |
| chr8.1 | exon | 11645833 | 11645901 | 11645833 | ID=MS.gene036757.t1.exon4;Parent=MS.gene036757.t1 |
| chr8.1 | CDS | 11645833 | 11645901 | 11645833 | ID=cds.MS.gene036757.t1;Parent=MS.gene036757.t1 |
| chr8.1 | exon | 11646000 | 11646047 | 11646000 | ID=MS.gene036757.t1.exon5;Parent=MS.gene036757.t1 |
| chr8.1 | CDS | 11646000 | 11646047 | 11646000 | ID=cds.MS.gene036757.t1;Parent=MS.gene036757.t1 |
| Gene Sequence |
| Protein sequence |
