AlfalfaGEDB | MS.gene036763

M. sativa cultivar XinJiangDaYe / MS.gene036763

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036763.t1	AES91544.2	96.4	55	2	0	1	55	1	55	2.30E-19	104

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036763.t1	Q9LDI3	74.5	55	14	0	1	55	1	55	2.6e-16	85.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036763.t1	A0A072USP3	96.4	55	2	0	1	55	1	55	1.6e-19	104.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036763.t1	MTR_4g114670	96.364	55	2	1	55	1	55	3.29e-30	108
MS.gene036763.t1	MTR_4g114670	96.364	55	2	1	55	1	55	5.77e-30	108
MS.gene036763.t1	MTR_4g114670	96.364	55	2	1	55	1	55	6.55e-30	108
MS.gene036763.t1	MTR_5g067150	71.154	52	15	4	55	2	53	1.22e-21	85.1
MS.gene036763.t1	MTR_5g067150	71.154	52	15	4	55	2	53	1.65e-21	85.1
MS.gene036763.t1	MTR_5g067150	71.154	52	15	4	55	2	53	2.36e-21	84.7
MS.gene036763.t1	MTR_5g088350	66.038	53	18	3	55	5	57	2.19e-20	81.6
MS.gene036763.t1	MTR_5g088350	64.815	54	19	2	55	4	57	3.62e-20	81.6
MS.gene036763.t1	MTR_5g088350	66.038	53	18	3	55	5	57	4.84e-20	81.3
MS.gene036763.t1	MTR_2g049790	66.667	51	17	5	55	17	67	3.34e-18	75.9
MS.gene036763.t1	MTR_8g024600	56.863	51	22	5	55	11	61	5.28e-15	67.0
MS.gene036763.t1	MTR_8g024590	56.863	51	22	5	55	11	61	7.22e-15	66.6
MS.gene036763.t1	MTR_4g128820	56.000	50	22	6	55	5	54	1.13e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036763.t1	AT5G35410	74.545	55	14	1	55	1	55	4.97e-23	89.7
MS.gene036763.t1	AT5G35410	74.545	55	14	1	55	1	55	1.50e-22	87.8
MS.gene036763.t1	AT2G26980	66.667	54	18	2	55	5	58	9.72e-22	84.3
MS.gene036763.t1	AT2G26980	66.667	54	18	2	55	5	58	1.69e-20	82.4
MS.gene036763.t1	AT2G26980	66.667	54	18	2	55	5	58	2.28e-20	82.0
MS.gene036763.t1	AT4G24400	71.154	52	15	4	55	2	53	7.96e-20	80.5
MS.gene036763.t1	AT2G26980	66.667	54	18	2	55	5	58	8.13e-20	80.5
MS.gene036763.t1	AT4G24400	71.154	52	15	4	55	2	53	9.56e-20	80.5
MS.gene036763.t1	AT2G26980	66.667	54	18	2	55	15	68	1.00e-19	80.5
MS.gene036763.t1	AT5G21326	61.111	54	21	2	55	4	57	9.59e-18	74.7
MS.gene036763.t1	AT1G30270	62.500	48	18	8	55	28	75	4.08e-16	70.1
MS.gene036763.t1	AT1G30270	62.500	48	18	8	55	28	75	4.15e-16	70.1
MS.gene036763.t1	AT1G01140	54.902	51	23	5	55	13	63	8.00e-16	69.3
MS.gene036763.t1	AT1G01140	54.902	51	23	5	55	13	63	8.07e-16	69.3
MS.gene036763.t1	AT1G01140	54.902	51	23	5	55	13	63	8.25e-16	69.3
MS.gene036763.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene036763.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene036763.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene036763.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene036763.t1	AT5G01810	60.870	46	18	10	55	11	56	2.80e-11	56.6
MS.gene036763.t1	AT5G01810	60.870	46	18	10	55	11	56	3.12e-11	56.2
MS.gene036763.t1	AT5G01810	60.870	46	18	10	55	11	56	3.12e-11	56.2
MS.gene036763.t1	AT3G17510	58.537	41	17	7	47	16	56	4.62e-11	55.8

Find 14 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAAGTGAGGAGAAAGAT+TGG	0.376571	8.1:+11858283	MS.gene036763:CDS
TATGAAGTTGGTAGAACAAT+TGG	0.383751	8.1:+11858310	MS.gene036763:CDS
CAAACACAGAATGGTTGAAC+AGG	0.443401	8.1:+11858423	MS.gene036763:CDS
AAGATTGGAAAGTATGAAGT+TGG	0.456314	8.1:+11858298	MS.gene036763:CDS
TTTGCAAAACACACCGAGAC+AGG	0.459686	8.1:+11858358	MS.gene036763:CDS
AACCATTCTGTGTTTGAGAA+TGG	0.473451	8.1:-11858416	None:intergenic
CGCGACACTTTCCCCTGTCT+CGG	0.504061	8.1:-11858371	None:intergenic
TACCATTCTCAAACACAGAA+TGG	0.543262	8.1:+11858414	MS.gene036763:CDS
TCAAAATAATGAAGAAAGTG+AGG	0.554812	8.1:+11858272	None:intergenic
TTGCAAAACACACCGAGACA+GGG	0.585182	8.1:+11858359	MS.gene036763:CDS
AAGTGTCGCGATTAAAGTCA+TGG	0.590091	8.1:+11858384	MS.gene036763:CDS
TGGTGAAGGTACATTCGCTA+AGG	0.613664	8.1:+11858330	MS.gene036763:CDS
GTTGGTAGAACAATTGGTGA+AGG	0.621321	8.1:+11858316	MS.gene036763:CDS
TGCAAAACACACCGAGACAG+GGG	0.649155	8.1:+11858360	MS.gene036763:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGATTGGAAAGTATGAAGT+TGG	+	chr8.1:11858298-11858317	MS.gene036763:CDS	30.0%
!!	TATGAAGTTGGTAGAACAAT+TGG	+	chr8.1:11858310-11858329	MS.gene036763:CDS	30.0%
	AACCATTCTGTGTTTGAGAA+TGG	-	chr8.1:11858419-11858438	None:intergenic	35.0%
	AAGAAAGTGAGGAGAAAGAT+TGG	+	chr8.1:11858283-11858302	MS.gene036763:CDS	35.0%
	TACCATTCTCAAACACAGAA+TGG	+	chr8.1:11858414-11858433	MS.gene036763:CDS	35.0%
!	AAGTGTCGCGATTAAAGTCA+TGG	+	chr8.1:11858384-11858403	MS.gene036763:CDS	40.0%
!	GTTGGTAGAACAATTGGTGA+AGG	+	chr8.1:11858316-11858335	MS.gene036763:CDS	40.0%
	TTGCAAAACACACCGAGACA+GGG	+	chr8.1:11858359-11858378	MS.gene036763:CDS	45.0%
	TTTGCAAAACACACCGAGAC+AGG	+	chr8.1:11858358-11858377	MS.gene036763:CDS	45.0%
!	TGGTGAAGGTACATTCGCTA+AGG	+	chr8.1:11858330-11858349	MS.gene036763:CDS	45.0%
	TGCAAAACACACCGAGACAG+GGG	+	chr8.1:11858360-11858379	MS.gene036763:CDS	50.0%
	CGCGACACTTTCCCCTGTCT+CGG	-	chr8.1:11858374-11858393	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	11858280	11858444	11858280	ID=MS.gene036763
chr8.1	mRNA	11858280	11858444	11858280	ID=MS.gene036763.t1;Parent=MS.gene036763
chr8.1	exon	11858280	11858444	11858280	ID=MS.gene036763.t1.exon1;Parent=MS.gene036763.t1
chr8.1	CDS	11858280	11858444	11858280	ID=cds.MS.gene036763.t1;Parent=MS.gene036763.t1

Gene Sequence	>MS.gene036763 ATGAAGAAAGTGAGGAGAAAGATTGGAAAGTATGAAGTTGGTAGAACAATTGGTGAAGGTACATTCGCTAAGGTCAAATTTGCAAAACACACCGAGACAGGGGAAAGTGTCGCGATTAAAGTCATGGCTAAAACTACCATTCTCAAACACAGAATGGTTGAACAG
Protein sequence	>MS.gene036763.t1 MKKVRRKIGKYEVGRTIGEGTFAKVKFAKHTETGESVAIKVMAKTTILKHRMVEQ