AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036815

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036815.t1 MTR_4g113940 78.598 271 50 2 1 264 1 270 2.18e-157 438
MS.gene036815.t1 MTR_4g113890 71.587 271 28 3 1 263 1 230 3.02e-124 353
MS.gene036815.t1 MTR_4g113860 47.403 308 117 6 1 264 1 307 8.70e-89 266
MS.gene036815.t1 MTR_4g113970 54.032 248 72 7 1 208 1 246 7.51e-78 236
MS.gene036815.t1 MTR_3g050390 46.250 240 97 8 31 264 15 228 2.21e-59 189
MS.gene036815.t1 MTR_8g066730 25.648 347 152 9 16 264 36 374 2.54e-26 105
MS.gene036815.t1 MTR_8g066730 27.843 255 113 6 16 207 36 282 2.63e-21 90.9
MS.gene036815.t1 MTR_3g466460 47.059 85 15 1 210 264 10 94 2.43e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036815.t1 AT3G19360 38.926 298 113 10 31 264 81 373 3.09e-50 169
MS.gene036815.t1 AT3G19360 38.926 298 113 10 31 264 81 373 3.09e-50 169
MS.gene036815.t1 AT1G32360 26.351 296 130 8 54 264 90 382 8.69e-24 99.4

Find 72 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATTGAAACTAATAATT+TGG 0.147683 8.1:-12647237 MS.gene036815:CDS
CCAACTAGCTTCTGCGAATT+TGG 0.173447 8.1:+12647452 None:intergenic
AGCCAGATCAATCGAATTTA+CGG 0.290260 8.1:-12644860 MS.gene036815:CDS
TCAATCGAATTTACGGTGAT+TGG 0.313071 8.1:-12644853 MS.gene036815:CDS
ATGAAGATCCTGCACAGTTT+AGG 0.341189 8.1:-12647298 MS.gene036815:CDS
CCAACTAAGAAGCGTATACT+TGG 0.350951 8.1:-12644893 MS.gene036815:CDS
TTCCAAATTATTAGTTTCAA+TGG 0.356757 8.1:+12647235 None:intergenic
ATTTGTACTAAGTTCAGATT+TGG 0.359410 8.1:-12647539 MS.gene036815:CDS
GAAGCACTCTCTTGATTTCA+AGG 0.359778 8.1:+12647265 None:intergenic
ATCGGCTGCAGCTGGAAAAT+CGG 0.361010 8.1:-12647415 MS.gene036815:CDS
CGACTAAACTGTGTCGCAAT+TGG 0.369653 8.1:-12647169 MS.gene036815:CDS
TGAACAACAATGACTTCAAA+AGG 0.386314 8.1:-12647658 MS.gene036815:CDS
TGCTATGGCAACTGCAGCTT+CGG 0.389652 8.1:+12644931 None:intergenic
AAATCAGAGTTACAAGTTCC+TGG 0.400098 8.1:-12644992 MS.gene036815:intron
AGATTTGGTGCTTGTAGAAA+CGG 0.437088 8.1:-12647524 MS.gene036815:CDS
AACTGCAGCATCAGCTTCAA+TGG 0.437500 8.1:+12644964 None:intergenic
AACTGCAGCTTCGGCTTCAA+TGG 0.440184 8.1:+12644940 None:intergenic
TTAGAGGTTGGAATATCATA+TGG 0.441570 8.1:+12647707 None:intergenic
TTGCGGCGGTGACATCTTAG+AGG 0.444480 8.1:+12647691 None:intergenic
CTGGTGCTCTTCCTTTGCTA+TGG 0.445302 8.1:+12644916 None:intergenic
CCAAGTATACGCTTCTTAGT+TGG 0.448187 8.1:+12644893 None:intergenic
TAACGATGATCGGCTGCAGC+TGG 0.448905 8.1:-12647423 MS.gene036815:CDS
AGCAAGCAAGGGGCACTTAT+CGG 0.455209 8.1:-12647193 MS.gene036815:CDS
CACCGTAAATTCGATTGATC+TGG 0.457971 8.1:+12644858 None:intergenic
TGTGCAAGCATTATTGCAAT+GGG 0.460308 8.1:-12647358 MS.gene036815:CDS
TCGGCTGCAGCTGGAAAATC+GGG 0.462737 8.1:-12647414 MS.gene036815:CDS
CTCTTTGCTCATGGTGAAGA+AGG 0.463092 8.1:-12647110 MS.gene036815:intron
CTGATCTGTTTGATTGAGGA+AGG 0.475163 8.1:+12647585 None:intergenic
AATATACACTTATCTCCATA+TGG 0.477192 8.1:+12647326 None:intergenic
GAGACAGGATATTGTTCCTA+TGG 0.480467 8.1:-12647143 MS.gene036815:CDS
CTCATGGAGCTGAAGAGATA+AGG 0.489689 8.1:-12647484 MS.gene036815:CDS
AATAATTGCAACTTTGCTCA+TGG 0.494959 8.1:-12647500 MS.gene036815:CDS
GATTGAGGAAGGGTCATCCT+TGG 0.496966 8.1:+12647596 None:intergenic
CTTGGAGGAGGGCATATCAA+TGG 0.505795 8.1:+12647614 None:intergenic
AGCTTCTGCGAATTTGGTGG+TGG 0.508361 8.1:+12647458 None:intergenic
GGAATGTGAACAAGCAAGCA+AGG 0.508839 8.1:-12647205 MS.gene036815:CDS
CTAATAATTTGGAAGGCAGC+AGG 0.521839 8.1:-12647226 MS.gene036815:CDS
GTATACGCTTCTTAGTTGGC+TGG 0.524014 8.1:+12644897 None:intergenic
AGGAGGGCATATCAATGGAT+TGG 0.527979 8.1:+12647619 None:intergenic
CCAAATTCGCAGAAGCTAGT+TGG 0.528628 8.1:-12647452 MS.gene036815:CDS
GGAAGGGTCATCCTTGGAGG+AGG 0.531078 8.1:+12647602 None:intergenic
TATTCATTCCTAAACTGTGC+AGG 0.531321 8.1:+12647290 None:intergenic
CTGTGCAAGCATTATTGCAA+TGG 0.534714 8.1:-12647359 MS.gene036815:CDS
TGTCGCAATTGGAAGGAGAC+AGG 0.541370 8.1:-12647158 MS.gene036815:CDS
ACTAGCTTCTGCGAATTTGG+TGG 0.541605 8.1:+12647455 None:intergenic
ATTGAAACTAATAATTTGGA+AGG 0.547647 8.1:-12647233 MS.gene036815:CDS
GAACAACAATGACTTCAAAA+GGG 0.550138 8.1:-12647657 MS.gene036815:CDS
AAAGAGACAGTTCTTTCCAT+AGG 0.554060 8.1:+12647127 None:intergenic
TGAGGAAGGGTCATCCTTGG+AGG 0.556539 8.1:+12647599 None:intergenic
CATTGATATGCCCTCCTCCA+AGG 0.556864 8.1:-12647613 MS.gene036815:CDS
GGCGGTGACATCTTAGAGGT+TGG 0.560713 8.1:+12647695 None:intergenic
TTGGTCCGTGTAACGATGAT+CGG 0.560743 8.1:-12647433 MS.gene036815:CDS
AACAATGACTTCAAAAGGGA+AGG 0.561307 8.1:-12647653 MS.gene036815:CDS
TGGTCTGATCTGTTTGATTG+AGG 0.566957 8.1:+12647581 None:intergenic
AAGAACTGTCTCTTTGCTCA+TGG 0.580294 8.1:-12647119 MS.gene036815:CDS
AATGGGGAGGAATGTCCATA+TGG 0.583975 8.1:-12647341 MS.gene036815:CDS
TCAGCTTCAATGGCTCCACC+AGG 0.589035 8.1:+12644974 None:intergenic
TAAACTGTGTCGCAATTGGA+AGG 0.596328 8.1:-12647165 MS.gene036815:CDS
GAATGTGAACAAGCAAGCAA+GGG 0.597583 8.1:-12647204 MS.gene036815:CDS
AGCTGCAGTTGCCATAGCAA+AGG 0.598992 8.1:-12644927 MS.gene036815:CDS
ACTGTGCAGGATCTTCATGA+AGG 0.599205 8.1:+12647303 None:intergenic
TGATCTGTTTGATTGAGGAA+GGG 0.618914 8.1:+12647586 None:intergenic
GAAGGGTCATCCTTGGAGGA+GGG 0.622595 8.1:+12647603 None:intergenic
AATATCATATGGAAAAGCCA+TGG 0.637338 8.1:+12647718 None:intergenic
CAACTAAGAAGCGTATACTT+GGG 0.638379 8.1:-12644892 MS.gene036815:CDS
TTGTTCAATGAAGTGAATTG+CGG 0.641834 8.1:+12647674 None:intergenic
AATGTGAACAAGCAAGCAAG+GGG 0.656040 8.1:-12647203 MS.gene036815:CDS
TTCAATGAAGTGAATTGCGG+CGG 0.657596 8.1:+12647677 None:intergenic
CAAGCATTATTGCAATGGGG+AGG 0.662472 8.1:-12647354 MS.gene036815:CDS
TGCAGCCGATCATCGTTACA+CGG 0.666217 8.1:+12647428 None:intergenic
TCAGAGTTACAAGTTCCTGG+TGG 0.718686 8.1:-12644989 MS.gene036815:intron
GTGCAAGCATTATTGCAATG+GGG 0.746686 8.1:-12647357 MS.gene036815:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGAAATAAAATACTAA+AGG + chr8.1:12646017-12646036 None:intergenic 10.0%
!! AATATCAAAAAATCATACAA+AGG - chr8.1:12645169-12645188 MS.gene036815:intron 15.0%
!! TATACTTTGATTATTTGTTT+CGG - chr8.1:12645479-12645498 MS.gene036815:intron 15.0%
!!! CAAGAGTTTTTTTAAATATA+AGG + chr8.1:12647413-12647432 None:intergenic 15.0%
!!! TTTTTTATTTCTTTTAATCC+TGG - chr8.1:12646366-12646385 MS.gene036815:intron 15.0%
!! AACCATTGAAACTAATAATT+TGG - chr8.1:12645320-12645339 MS.gene036815:intron 20.0%
!! ATTGAAACTAATAATTTGGA+AGG - chr8.1:12645324-12645343 MS.gene036815:intron 20.0%
!!! GTTTTGTACTAAATAAAGTT+TGG - chr8.1:12646867-12646886 MS.gene036815:intron 20.0%
!!! TTCCAAATTATTAGTTTCAA+TGG + chr8.1:12645325-12645344 None:intergenic 20.0%
! AAAAATCTGGCATATAAACA+TGG + chr8.1:12646699-12646718 None:intergenic 25.0%
! AAAATACTAAAGGAAGAAGT+AGG + chr8.1:12646007-12646026 None:intergenic 25.0%
! AAGTTATCATAAGCAAAATG+CGG + chr8.1:12646738-12646757 None:intergenic 25.0%
! AATATACACTTATCTCCATA+TGG + chr8.1:12645234-12645253 None:intergenic 25.0%
! AATGATCCATTAGAATAAAG+AGG + chr8.1:12647044-12647063 None:intergenic 25.0%
! AATGGATCATTACTATTGTA+TGG - chr8.1:12647053-12647072 MS.gene036815:intron 25.0%
! ATCAAATTTCTGCCATAAAT+GGG - chr8.1:12646594-12646613 MS.gene036815:intron 25.0%
! ATGATCCATTAGAATAAAGA+GGG + chr8.1:12647043-12647062 None:intergenic 25.0%
! ATTTGTACTAAGTTCAGATT+TGG - chr8.1:12645018-12645037 MS.gene036815:intron 25.0%
! CATGGCAATTAAAAATTCAA+TGG + chr8.1:12646392-12646411 None:intergenic 25.0%
! TATAAAAATTCCTGACAATG+CGG + chr8.1:12646978-12646997 None:intergenic 25.0%
! TATCAAATTTCTGCCATAAA+TGG - chr8.1:12646593-12646612 MS.gene036815:intron 25.0%
! TCTTAACAAATGTAAAACTG+TGG + chr8.1:12647103-12647122 None:intergenic 25.0%
! TGATCATTAATCATACATGA+AGG + chr8.1:12646559-12646578 None:intergenic 25.0%
! TGTACTAAATAAAGTTTGGT+GGG - chr8.1:12646871-12646890 MS.gene036815:intron 25.0%
! TTATCTTGCAAAAATGAGTT+GGG - chr8.1:12645717-12645736 MS.gene036815:intron 25.0%
! TTGTACTAAATAAAGTTTGG+TGG - chr8.1:12646870-12646889 MS.gene036815:intron 25.0%
!! ACTTTAAGAAGAAACAAAGT+TGG + chr8.1:12647198-12647217 None:intergenic 25.0%
!! TGACATGAACAATAATTTTG+AGG - chr8.1:12647250-12647269 MS.gene036815:CDS 25.0%
!!! TACTAGAATTTGTTGTAGTT+TGG - chr8.1:12647123-12647142 MS.gene036815:CDS 25.0%
AATAATTGCAACTTTGCTCA+TGG - chr8.1:12645057-12645076 MS.gene036815:intron 30.0%
AGTGGCGCATAAAAAATAAA+TGG - chr8.1:12645797-12645816 MS.gene036815:intron 30.0%
ATGTCAGACATCAAATTTGT+AGG + chr8.1:12647236-12647255 None:intergenic 30.0%
CAATTAAAAATTCAATGGCC+AGG + chr8.1:12646387-12646406 None:intergenic 30.0%
CTTATCTTGCAAAAATGAGT+TGG - chr8.1:12645716-12645735 MS.gene036815:intron 30.0%
GAACAACAATGACTTCAAAA+GGG - chr8.1:12644900-12644919 MS.gene036815:CDS 30.0%
GATGAACATAATACAATAGC+TGG + chr8.1:12647171-12647190 None:intergenic 30.0%
TAAATGGACAGACATAGAAA+TGG - chr8.1:12646439-12646458 MS.gene036815:intron 30.0%
TGAACAACAATGACTTCAAA+AGG - chr8.1:12644899-12644918 MS.gene036815:CDS 30.0%
TGATCCATTAGAATAAAGAG+GGG + chr8.1:12647042-12647061 None:intergenic 30.0%
TGCAAATTACAGTGTTTGAA+TGG - chr8.1:12645598-12645617 MS.gene036815:intron 30.0%
TGTGTATGATGTTTCATGTT+TGG - chr8.1:12645653-12645672 MS.gene036815:intron 30.0%
TTAAAACAACTGCAACTGTT+TGG - chr8.1:12647497-12647516 MS.gene036815:CDS 30.0%
TTAGAGGTTGGAATATCATA+TGG + chr8.1:12644853-12644872 None:intergenic 30.0%
TTGTTCAATGAAGTGAATTG+CGG + chr8.1:12644886-12644905 None:intergenic 30.0%
! ATGATTCCATCCTTGTTTAT+AGG - chr8.1:12646513-12646532 MS.gene036815:intron 30.0%
! ATGGCAGAAATTTGATAACA+AGG + chr8.1:12646590-12646609 None:intergenic 30.0%
! ATGTAAGTGCTGTTAAATAG+CGG + chr8.1:12646919-12646938 None:intergenic 30.0%
! ATTTTCCATTCAGAACCTTA+GGG + chr8.1:12646495-12646514 None:intergenic 30.0%
! ATTTTTGCAAGATAAGGCAA+TGG + chr8.1:12645712-12645731 None:intergenic 30.0%
! GTACTAAATAAAGTTTGGTG+GGG - chr8.1:12646872-12646891 MS.gene036815:intron 30.0%
! TTCGTGTTTTGTAGAAAATG+TGG + chr8.1:12644999-12645018 None:intergenic 30.0%
! TTTTTGCAAGATAAGGCAAT+GGG + chr8.1:12645711-12645730 None:intergenic 30.0%
!! CAACTCATTTTTGCAAGATA+AGG + chr8.1:12645718-12645737 None:intergenic 30.0%
AAAGAGACAGTTCTTTCCAT+AGG + chr8.1:12645433-12645452 None:intergenic 35.0%
AAATCAGAGTTACAAGTTCC+TGG - chr8.1:12647565-12647584 MS.gene036815:CDS 35.0%
AACAATGACTTCAAAAGGGA+AGG - chr8.1:12644904-12644923 MS.gene036815:CDS 35.0%
AGCCAGATCAATCGAATTTA+CGG - chr8.1:12647697-12647716 MS.gene036815:CDS 35.0%
AGGAGTCAGAACAATATACA+TGG + chr8.1:12647526-12647545 None:intergenic 35.0%
ATAGTCTATACCATGATACC+CGG - chr8.1:12646648-12646667 MS.gene036815:intron 35.0%
CATACATCTAAGCAAAGTAC+CGG + chr8.1:12645774-12645793 None:intergenic 35.0%
CATATTTGACCCCAATATTG+AGG + chr8.1:12647321-12647340 None:intergenic 35.0%
CTCAATATTGGGGTCAAATA+TGG - chr8.1:12647319-12647338 MS.gene036815:CDS 35.0%
GATCCATTAGAATAAAGAGG+GGG + chr8.1:12647041-12647060 None:intergenic 35.0%
GGCGCATAAAAAATAAATGG+TGG - chr8.1:12645800-12645819 MS.gene036815:intron 35.0%
TAACCCCCTCTTTATTCTAA+TGG - chr8.1:12647035-12647054 MS.gene036815:intron 35.0%
TATTCATTCCTAAACTGTGC+AGG + chr8.1:12645270-12645289 None:intergenic 35.0%
TCAATCGAATTTACGGTGAT+TGG - chr8.1:12647704-12647723 MS.gene036815:CDS 35.0%
TGAGAAGAAGAACAGTGAAA+GGG + chr8.1:12645846-12645865 None:intergenic 35.0%
TGATCTGTTTGATTGAGGAA+GGG + chr8.1:12644974-12644993 None:intergenic 35.0%
TGTGCAAGCATTATTGCAAT+GGG - chr8.1:12645199-12645218 MS.gene036815:intron 35.0%
TTGAGAAGAAGAACAGTGAA+AGG + chr8.1:12645847-12645866 None:intergenic 35.0%
TTGCTTAGATGTATGACAAG+TGG - chr8.1:12645779-12645798 MS.gene036815:intron 35.0%
TTGTGGTTTCACAAATCTAC+CGG - chr8.1:12646146-12646165 MS.gene036815:intron 35.0%
! AAAATGCGGATTAAGGTATC+TGG + chr8.1:12646724-12646743 None:intergenic 35.0%
! ATGTTGTTAATTGCTGTTCG+CGG - chr8.1:12646780-12646799 MS.gene036815:intron 35.0%
! CAACTAAGAAGCGTATACTT+GGG - chr8.1:12647665-12647684 MS.gene036815:CDS 35.0%
! CATAAGCAAAATGCGGATTA+AGG + chr8.1:12646731-12646750 None:intergenic 35.0%
! CATTTTCCATTCAGAACCTT+AGG + chr8.1:12646496-12646515 None:intergenic 35.0%
! TGTAAGTGCTGTTAAATAGC+GGG + chr8.1:12646918-12646937 None:intergenic 35.0%
!! AGATTTGGTGCTTGTAGAAA+CGG - chr8.1:12645033-12645052 MS.gene036815:intron 35.0%
AAACTGGAGGAAATCACTAC+TGG + chr8.1:12645750-12645769 None:intergenic 40.0%
AAGAACTGTCTCTTTGCTCA+TGG - chr8.1:12645438-12645457 MS.gene036815:intron 40.0%
AATGTGAACAAGCAAGCAAG+GGG - chr8.1:12645354-12645373 MS.gene036815:intron 40.0%
ACATAGAAATGGAGCAGATC+TGG - chr8.1:12646450-12646469 MS.gene036815:intron 40.0%
ACCTGCTATGCCTATAAACA+AGG + chr8.1:12646526-12646545 None:intergenic 40.0%
AGATGAGTCTCCTTTGTTCA+CGG - chr8.1:12646322-12646341 MS.gene036815:intron 40.0%
ATGAAGATCCTGCACAGTTT+AGG - chr8.1:12645259-12645278 MS.gene036815:intron 40.0%
ATGCATCTGTAAAGAGAACC+GGG - chr8.1:12646416-12646435 MS.gene036815:intron 40.0%
ATGCCGTGATCCTCAATATT+GGG - chr8.1:12647308-12647327 MS.gene036815:CDS 40.0%
CACCGTAAATTCGATTGATC+TGG + chr8.1:12647702-12647721 None:intergenic 40.0%
CATGCATCTGTAAAGAGAAC+CGG - chr8.1:12646415-12646434 MS.gene036815:intron 40.0%
CCAAGTATACGCTTCTTAGT+TGG + chr8.1:12647667-12647686 None:intergenic 40.0%
CTAATAATTTGGAAGGCAGC+AGG - chr8.1:12645331-12645350 MS.gene036815:intron 40.0%
CTGATCTGTTTGATTGAGGA+AGG + chr8.1:12644975-12644994 None:intergenic 40.0%
CTGTGCAAGCATTATTGCAA+TGG - chr8.1:12645198-12645217 MS.gene036815:intron 40.0%
CTTTACAGATGCATGACACA+TGG + chr8.1:12646410-12646429 None:intergenic 40.0%
GAATGTGAACAAGCAAGCAA+GGG - chr8.1:12645353-12645372 MS.gene036815:intron 40.0%
GAGACAGGATATTGTTCCTA+TGG - chr8.1:12645414-12645433 MS.gene036815:intron 40.0%
GCTATGCCTATAAACAAGGA+TGG + chr8.1:12646522-12646541 None:intergenic 40.0%
GTGCAAGCATTATTGCAATG+GGG - chr8.1:12645200-12645219 MS.gene036815:intron 40.0%
GTTGTATGTTGTTAGCGTTG+TGG - chr8.1:12646129-12646148 MS.gene036815:intron 40.0%
TAAACTGTGTCGCAATTGGA+AGG - chr8.1:12645392-12645411 MS.gene036815:intron 40.0%
TCTATAGCACTATTGCAGAG+CGG + chr8.1:12646828-12646847 None:intergenic 40.0%
TCTATGTCTGTCCATTTACC+CGG + chr8.1:12646437-12646456 None:intergenic 40.0%
TGGTCTGATCTGTTTGATTG+AGG + chr8.1:12644979-12644998 None:intergenic 40.0%
TGTAAAGAGAACCGGGTAAA+TGG - chr8.1:12646423-12646442 MS.gene036815:intron 40.0%
TGTTCTGACTCCTGAAGTAT+CGG - chr8.1:12647533-12647552 MS.gene036815:CDS 40.0%
TTCAATGAAGTGAATTGCGG+CGG + chr8.1:12644883-12644902 None:intergenic 40.0%
! AAGGTATCTGGCGAAAAATC+TGG + chr8.1:12646712-12646731 None:intergenic 40.0%
! AGTGATTTCCTCCAGTTTTC+CGG - chr8.1:12645752-12645771 MS.gene036815:intron 40.0%
! CCAACTAAGAAGCGTATACT+TGG - chr8.1:12647664-12647683 MS.gene036815:CDS 40.0%
! GAAGCACTCTCTTGATTTCA+AGG + chr8.1:12645295-12645314 None:intergenic 40.0%
! TCCTTGTTTATAGGCATAGC+AGG - chr8.1:12646522-12646541 MS.gene036815:intron 40.0%
!! TAAGCAAAGTACCGGAAAAC+TGG + chr8.1:12645766-12645785 None:intergenic 40.0%
AAACCCCATTCCGTGAACAA+AGG + chr8.1:12646335-12646354 None:intergenic 45.0%
AACTGCAGCATCAGCTTCAA+TGG + chr8.1:12647596-12647615 None:intergenic 45.0%
AAGGCAATGGGACAACAAGT+AGG + chr8.1:12645699-12645718 None:intergenic 45.0%
AATATCCCTGTCACACAGCA+TGG - chr8.1:12646242-12646261 MS.gene036815:intron 45.0%
AATGGGGAGGAATGTCCATA+TGG - chr8.1:12645216-12645235 MS.gene036815:intron 45.0%
ACTGTGCAGGATCTTCATGA+AGG + chr8.1:12645257-12645276 None:intergenic 45.0%
AGTCTCCTTTGTTCACGGAA+TGG - chr8.1:12646327-12646346 MS.gene036815:intron 45.0%
ATGGAAGCTGTGACATGTGA+AGG - chr8.1:12646261-12646280 MS.gene036815:intron 45.0%
ATTCCTGACAATGCGGTTGA+TGG + chr8.1:12646971-12646990 None:intergenic 45.0%
CAAGCATTATTGCAATGGGG+AGG - chr8.1:12645203-12645222 MS.gene036815:intron 45.0%
CAATATTGAGGATCACGGCA+TGG + chr8.1:12647309-12647328 None:intergenic 45.0%
CATGCCGTGATCCTCAATAT+TGG - chr8.1:12647307-12647326 MS.gene036815:CDS 45.0%
CCAAATTCGCAGAAGCTAGT+TGG - chr8.1:12645105-12645124 MS.gene036815:intron 45.0%
CGACTAAACTGTGTCGCAAT+TGG - chr8.1:12645388-12645407 MS.gene036815:intron 45.0%
CTCATGGAGCTGAAGAGATA+AGG - chr8.1:12645073-12645092 MS.gene036815:intron 45.0%
CTCTTTGCTCATGGTGAAGA+AGG - chr8.1:12645447-12645466 MS.gene036815:intron 45.0%
GACACACCATTACAGAAGGA+GGG + chr8.1:12645892-12645911 None:intergenic 45.0%
GACCCCAATATTGAGGATCA+CGG + chr8.1:12647314-12647333 None:intergenic 45.0%
GGAATGTGAACAAGCAAGCA+AGG - chr8.1:12645352-12645371 MS.gene036815:intron 45.0%
GGCATATAAACATGGAGCTG+CGG + chr8.1:12646691-12646710 None:intergenic 45.0%
GTATACGCTTCTTAGTTGGC+TGG + chr8.1:12647663-12647682 None:intergenic 45.0%
GTCTCCTTTGTTCACGGAAT+GGG - chr8.1:12646328-12646347 MS.gene036815:intron 45.0%
TCAGAGTTACAAGTTCCTGG+TGG - chr8.1:12647568-12647587 MS.gene036815:CDS 45.0%
TCTCCTTTGTTCACGGAATG+GGG - chr8.1:12646329-12646348 MS.gene036815:intron 45.0%
TGACACACCATTACAGAAGG+AGG + chr8.1:12645893-12645912 None:intergenic 45.0%
TGCAAGAATGTACCTCCCTA+AGG - chr8.1:12646477-12646496 MS.gene036815:intron 45.0%
TGCAAGACAACCGATACTTC+AGG + chr8.1:12647546-12647565 None:intergenic 45.0%
TGCCGTGATCCTCAATATTG+GGG - chr8.1:12647309-12647328 MS.gene036815:CDS 45.0%
TGTGACATGTGAAGGCCAAA+CGG - chr8.1:12646269-12646288 MS.gene036815:intron 45.0%
TTCCATTCAGAACCTTAGGG+AGG + chr8.1:12646492-12646511 None:intergenic 45.0%
TTCCTGACAATGCGGTTGAT+GGG + chr8.1:12646970-12646989 None:intergenic 45.0%
TTGGTCCGTGTAACGATGAT+CGG - chr8.1:12645124-12645143 MS.gene036815:intron 45.0%
! ACTAGCTTCTGCGAATTTGG+TGG + chr8.1:12645105-12645124 None:intergenic 45.0%
! AGGAGGGCATATCAATGGAT+TGG + chr8.1:12644941-12644960 None:intergenic 45.0%
! CCAACTAGCTTCTGCGAATT+TGG + chr8.1:12645108-12645127 None:intergenic 45.0%
! TTGCTGTTCGCGGAAAAATG+TGG - chr8.1:12646790-12646809 MS.gene036815:intron 45.0%
!! TACCTCCCTAAGGTTCTGAA+TGG - chr8.1:12646487-12646506 MS.gene036815:intron 45.0%
!! TTTCTGCCATAAATGGGCTG+TGG - chr8.1:12646600-12646619 MS.gene036815:intron 45.0%
!!! AAGAGTGACGGGTTTTCACA+GGG + chr8.1:12645919-12645938 None:intergenic 45.0%
AAACATGGAGCTGCGGCATA+TGG + chr8.1:12646684-12646703 None:intergenic 50.0%
AACTCGTAACGACGTGCCAT+TGG + chr8.1:12645966-12645985 None:intergenic 50.0%
AACTGCAGCTTCGGCTTCAA+TGG + chr8.1:12647620-12647639 None:intergenic 50.0%
AGCTGCAGTTGCCATAGCAA+AGG - chr8.1:12647630-12647649 MS.gene036815:CDS 50.0%
AGCTTCCATGCTGTGTGACA+GGG + chr8.1:12646250-12646269 None:intergenic 50.0%
ATCGGCTGCAGCTGGAAAAT+CGG - chr8.1:12645142-12645161 MS.gene036815:intron 50.0%
CATTGATATGCCCTCCTCCA+AGG - chr8.1:12644944-12644963 MS.gene036815:CDS 50.0%
CCACCACTTAAATCCCCCAA+TGG - chr8.1:12645947-12645966 MS.gene036815:intron 50.0%
CCATTACAGAAGGAGGGAGA+AGG + chr8.1:12645886-12645905 None:intergenic 50.0%
CCATTGGGGGATTTAAGTGG+TGG + chr8.1:12645950-12645969 None:intergenic 50.0%
CCTTCTCCCTCCTTCTGTAA+TGG - chr8.1:12645883-12645902 MS.gene036815:intron 50.0%
CGGTGACACACCATTACAGA+AGG + chr8.1:12645896-12645915 None:intergenic 50.0%
CTGGTGCTCTTCCTTTGCTA+TGG + chr8.1:12647644-12647663 None:intergenic 50.0%
GATTGAGGAAGGGTCATCCT+TGG + chr8.1:12644964-12644983 None:intergenic 50.0%
GCAATGGGACAACAAGTAGG+AGG + chr8.1:12645696-12645715 None:intergenic 50.0%
TGCAGCCGATCATCGTTACA+CGG + chr8.1:12645132-12645151 None:intergenic 50.0%
TGCTATGGCAACTGCAGCTT+CGG + chr8.1:12647629-12647648 None:intergenic 50.0%
TGGAAACAAGCCGGGTATCA+TGG + chr8.1:12646661-12646680 None:intergenic 50.0%
TGTCGCAATTGGAAGGAGAC+AGG - chr8.1:12645399-12645418 MS.gene036815:intron 50.0%
TTCACGGAATGGGGTTTCAC+AGG - chr8.1:12646338-12646357 MS.gene036815:intron 50.0%
TTCCCATCAACCGCATTGTC+AGG - chr8.1:12646965-12646984 MS.gene036815:intron 50.0%
! ACTCGTAACGACGTGCCATT+GGG + chr8.1:12645965-12645984 None:intergenic 50.0%
! AGCTTCTGCGAATTTGGTGG+TGG + chr8.1:12645102-12645121 None:intergenic 50.0%
! CCTACGACGGAACACTTTTC+CGG + chr8.1:12646193-12646212 None:intergenic 50.0%
! CTTGGAGGAGGGCATATCAA+TGG + chr8.1:12644946-12644965 None:intergenic 50.0%
! GCATATGGTGGAAACAAGCC+GGG + chr8.1:12646669-12646688 None:intergenic 50.0%
! GGCATATGGTGGAAACAAGC+CGG + chr8.1:12646670-12646689 None:intergenic 50.0%
! GTGCCATTGGGGGATTTAAG+TGG + chr8.1:12645953-12645972 None:intergenic 50.0%
! GTGGTGACTTGCAAGAGTGA+CGG + chr8.1:12645931-12645950 None:intergenic 50.0%
! TGGTGACTTGCAAGAGTGAC+GGG + chr8.1:12645930-12645949 None:intergenic 50.0%
!! AGCAAGCAAGGGGCACTTAT+CGG - chr8.1:12645364-12645383 MS.gene036815:intron 50.0%
!! ATAAATGGGCTGTGGCATGG+CGG - chr8.1:12646608-12646627 MS.gene036815:intron 50.0%
!! ATGGCGGACTTTGTGCTAGT+CGG - chr8.1:12646624-12646643 MS.gene036815:intron 50.0%
!! GAAAAGTGTTCCGTCGTAGG+TGG - chr8.1:12646193-12646212 MS.gene036815:intron 50.0%
!! GCAAAGTACCGGAAAACTGG+AGG + chr8.1:12645763-12645782 None:intergenic 50.0%
!!! CAAGAGTGACGGGTTTTCAC+AGG + chr8.1:12645920-12645939 None:intergenic 50.0%
AAGGTCTGCGACCATGACTG+TGG + chr8.1:12647394-12647413 None:intergenic 55.0%
AAGTGTTCCGTCGTAGGTGG+CGG - chr8.1:12646196-12646215 MS.gene036815:intron 55.0%
CAGAAGGAGGGAGAAGGAGA+AGG + chr8.1:12645880-12645899 None:intergenic 55.0%
CAGCTTCCATGCTGTGTGAC+AGG + chr8.1:12646251-12646270 None:intergenic 55.0%
GAAGGGTCATCCTTGGAGGA+GGG + chr8.1:12644957-12644976 None:intergenic 55.0%
GACATGTGAAGGCCAAACGG+CGG - chr8.1:12646272-12646291 MS.gene036815:intron 55.0%
GCCATGCCACAGCCCATTTA+TGG + chr8.1:12646609-12646628 None:intergenic 55.0%
GGCGGTGACATCTTAGAGGT+TGG + chr8.1:12644865-12644884 None:intergenic 55.0%
TAACGATGATCGGCTGCAGC+TGG - chr8.1:12645134-12645153 MS.gene036815:intron 55.0%
TCAGCTTCAATGGCTCCACC+AGG + chr8.1:12647586-12647605 None:intergenic 55.0%
TCGGCTGCAGCTGGAAAATC+GGG - chr8.1:12645143-12645162 MS.gene036815:intron 55.0%
TCTCAGCCACACCACAGTCA+TGG - chr8.1:12647380-12647399 MS.gene036815:CDS 55.0%
TGAGGAAGGGTCATCCTTGG+AGG + chr8.1:12644961-12644980 None:intergenic 55.0%
TTGCGGCGGTGACATCTTAG+AGG + chr8.1:12644869-12644888 None:intergenic 55.0%
! CTCGTAACGACGTGCCATTG+GGG + chr8.1:12645964-12645983 None:intergenic 55.0%
! TCGTAACGACGTGCCATTGG+GGG + chr8.1:12645963-12645982 None:intergenic 55.0%
!! CCGGAAAAGTGTTCCGTCGT+AGG - chr8.1:12646190-12646209 MS.gene036815:intron 55.0%
!! GCCATAAATGGGCTGTGGCA+TGG - chr8.1:12646605-12646624 MS.gene036815:intron 55.0%
!!! AGTGACGGGTTTTCACAGGG+CGG + chr8.1:12645916-12645935 None:intergenic 55.0%
AGTGTTCCGTCGTAGGTGGC+GGG - chr8.1:12646197-12646216 MS.gene036815:intron 60.0%
CATGGAGCTGCGGCATATGG+TGG + chr8.1:12646681-12646700 None:intergenic 60.0%
CTGCGACCATGACTGTGGTG+TGG + chr8.1:12647389-12647408 None:intergenic 60.0%
GGAAGGGTCATCCTTGGAGG+AGG + chr8.1:12644958-12644977 None:intergenic 60.0%
!! CAAATCTACCGGCTCCGCTG+CGG - chr8.1:12646157-12646176 MS.gene036815:intron 60.0%
AAGGCCAAACGGCGGCGATG+CGG - chr8.1:12646280-12646299 MS.gene036815:intron 65.0%
CCGCTGCGGTGATCGGAATC+CGG - chr8.1:12646171-12646190 MS.gene036815:intron 65.0%
CCGGATTCCGATCACCGCAG+CGG + chr8.1:12646174-12646193 None:intergenic 65.0%
CCTTTCCCCGCCACCTACGA+CGG + chr8.1:12646206-12646225 None:intergenic 65.0%
GCGGCGATGCGGTGGCTAAA+CGG - chr8.1:12646291-12646310 MS.gene036815:intron 65.0%
GTGTTCCGTCGTAGGTGGCG+GGG - chr8.1:12646198-12646217 MS.gene036815:intron 65.0%
TCCGATCACCGCAGCGGAGC+CGG + chr8.1:12646168-12646187 None:intergenic 70.0%
! ACCGGCTCCGCTGCGGTGAT+CGG - chr8.1:12646164-12646183 MS.gene036815:intron 70.0%
!! CCGTCGTAGGTGGCGGGGAA+AGG - chr8.1:12646203-12646222 MS.gene036815:intron 70.0%
GCCAAACGGCGGCGATGCGG+TGG - chr8.1:12646283-12646302 MS.gene036815:intron 75.0%
GCCACCGCATCGCCGCCGTT+TGG + chr8.1:12646287-12646306 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 12644841 12647738 12644841 ID=MS.gene036815
chr8.1 mRNA 12644841 12647738 12644841 ID=MS.gene036815.t1;Parent=MS.gene036815
chr8.1 exon 12647111 12647738 12647111 ID=MS.gene036815.t1.exon1;Parent=MS.gene036815.t1
chr8.1 CDS 12647111 12647738 12647111 ID=cds.MS.gene036815.t1;Parent=MS.gene036815.t1
chr8.1 exon 12644841 12645007 12644841 ID=MS.gene036815.t1.exon2;Parent=MS.gene036815.t1
chr8.1 CDS 12644841 12645007 12644841 ID=cds.MS.gene036815.t1;Parent=MS.gene036815.t1
Gene Sequence

>MS.gene036815

ATGGCTTTTCCATATGATATTCCAACCTCTAAGATGTCACCGCCGCAATTCACTTCATTGAACAACAATGACTTCAAAAGGGAAGGAACTTCTCATTCCAATCCATTGATATGCCCTCCTCCAAGGATGACCCTTCCTCAATCAAACAGATCAGACCACATTTTCTACAAAACACGAATTTGTACTAAGTTCAGATTTGGTGCTTGTAGAAACGGAAATAATTGCAACTTTGCTCATGGAGCTGAAGAGATAAGGCAACCACCACCAAATTCGCAGAAGCTAGTTGGTCCGTGTAACGATGATCGGCTGCAGCTGGAAAATCGGGCTGAATATCAAAAAATCATACAAAGGATGAAACTGTGCAAGCATTATTGCAATGGGGAGGAATGTCCATATGGAGATAAGTGTATATTCCTTCATGAAGATCCTGCACAGTTTAGGAATGAATATTCCTTGAAATCAAGAGAGTGCTTCGCAATAACCATTGAAACTAATAATTTGGAAGGCAGCAGGAATGTGAACAAGCAAGCAAGGGGCACTTATCGGACGACTAAACTGTGTCGCAATTGGAAGGAGACAGGATATTGTTCCTATGGAAAGAACTGTCTCTTTGCTCATGGTGAAGAAGGTAACAATAATATACTTTGATTATTTGTTTCGGTTTTAACATATAATTCTATTGCTTTATTTTGTTTAATTATATAAAATATGTTATTTTCTAGATGCTGTGTATAATGTCATTTTCTGTGATATGATGTGCAAATTACAGTGTTTGAATGGTTTGAATGTGTGTCATTTTGTTGTTGTTGTGTTGTGTATGATGTTTCATGTTTGGTTTGACATAAACTTGCTCCTCCTACTTGTTGTCCCATTGCCTTATCTTGCAAAAATGAGTTGGGATCTGCTCCAGTAGTGATTTCCTCCAGTTTTCCGGTACTTTGCTTAGATGTATGACAAGTGGCGCATAAAAAATAAATGGTGGAGATTAAAAAAACAAAATAACCCTTTCACTGTTCTTCTTCTCAATTGTACGCTTCCTTCTCCTTCTCCCTCCTTCTGTAATGGTGTGTCACCGCCCTGTGAAAACCCGTCACTCTTGCAAGTCACCACCACTTAAATCCCCCAATGGCACGTCGTTACGAGTTGCTGCATTTCAAATGTTTCCTACTTCTTCCTTTAGTATTTTATTTCAATTTCTAGAATTCAGAAACTAATTTATTTCAATTTTCAGAATTCTAAAACAGAAAACGATTATGAAATCAGACTAGCATGCTTCAAAATCAAAGTTGTTGTATGTTGTTAGCGTTGTGGTTTCACAAATCTACCGGCTCCGCTGCGGTGATCGGAATCCGGAAAAGTGTTCCGTCGTAGGTGGCGGGGAAAGGAAAAGATGACGACGCGAATATCCCTGTCACACAGCATGGAAGCTGTGACATGTGAAGGCCAAACGGCGGCGATGCGGTGGCTAAACGGTGTTTTAGAGATGAGTCTCCTTTGTTCACGGAATGGGGTTTCACAGGAATCATTTTTTATTTCTTTTAATCCTGGCCATTGAATTTTTAATTGCCATGTGTCATGCATCTGTAAAGAGAACCGGGTAAATGGACAGACATAGAAATGGAGCAGATCTGGACTCTGCAAGAATGTACCTCCCTAAGGTTCTGAATGGAAAATGATTCCATCCTTGTTTATAGGCATAGCAGGTTCTGAATGTACCTTCATGTATGATTAATGATCACATTATCTCCTTGTTATCAAATTTCTGCCATAAATGGGCTGTGGCATGGCGGACTTTGTGCTAGTCGGCATAGTCTATACCATGATACCCGGCTTGTTTCCACCATATGCCGCAGCTCCATGTTTATATGCCAGATTTTTCGCCAGATACCTTAATCCGCATTTTGCTTATGATAACTTTGCTCTAGCTGAGATTTACATTATGTTGTTAATTGCTGTTCGCGGAAAAATGTGGTTAGTTCGAATTCCGCTCTGCAATAGTGCTATAGACGCTATTTCACAATATTTTGTTTTGTACTAAATAAAGTTTGGTGGGGCAATAGTCATTTGTTCAAATCCCGCTATTTAACAGCACTTACATAATACTTTTGAATGAAATTCTGAATTTTCCCATCAACCGCATTGTCAGGAATTTTTATATTATAGAAATGTCTAAGTTTTCATAAATTAAAATAGCTAACCCCCTCTTTATTCTAATGGATCATTACTATTGTATGGTGTTGAAAGTTAAGAGAAAAAAATCCACAGTTTTACATTTGTTAAGATACTAGAATTTGTTGTAGTTTGGAGTAATTTGTGATTCTTAATAACCAGCTATTGTATTATGTTCATCTTTTCCAACTTTGTTTCTTCTTAAAGTTTTTGTTGATAATTGCCTACAAATTTGATGTCTGACATGAACAATAATTTTGAGGTACATTGCTCATTTGCAAAGTTTTAAATTGCGACCATGCCGTGATCCTCAATATTGGGGTCAAATATGGTTAATGCAGATGCATTTGTGTGTGAATGTATGATAACGTCTCAGCCACACCACAGTCATGGTCGCAGACCTTATATTTAAAAAAACTCTTGCTCGTTTGTGTGCGAGTGTATGATAACTTATATTTGCATATTGTAAGCGAAAATTACAACATGATTAAAACAACTGCAACTGTTTGGTTTCCATGTATATTGTTCTGACTCCTGAAGTATCGGTTGTCTTGCAAATCAGAGTTACAAGTTCCTGGTGGAGCCATTGAAGCTGATGCTGCAGTTGCCATTGAAGCCGAAGCTGCAGTTGCCATAGCAAAGGAAGAGCACCAGCCAACTAAGAAGCGTATACTTGGGCTGAAATTCAGCCAGATCAATCGAATTTACGGTGATTGGATTGATGATTGA
Protein sequence

>MS.gene036815.t1

MAFPYDIPTSKMSPPQFTSLNNNDFKREGTSHSNPLICPPPRMTLPQSNRSDHIFYKTRICTKFRFGACRNGNNCNFAHGAEEIRQPPPNSQKLVGPCNDDRLQLENRAEYQKIIQRMKLCKHYCNGEECPYGDKCIFLHEDPAQFRNEYSLKSRECFAITIETNNLEGSRNVNKQARGTYRTTKLCRNWKETGYCSYGKNCLFAHGEEELQVPGGAIEADAAVAIEAEAAVAIAKEEHQPTKKRILGLKFSQINRIYGDWIDD