AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036821

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036821.t1 MTR_4g113940 72.963 270 33 2 1 230 1 270 1.63e-139 392
MS.gene036821.t1 MTR_4g113890 84.810 237 21 2 1 229 1 230 5.34e-139 389
MS.gene036821.t1 MTR_4g113860 45.130 308 90 7 1 230 1 307 2.74e-81 246
MS.gene036821.t1 MTR_4g113970 45.344 247 54 6 1 167 1 246 2.18e-58 185
MS.gene036821.t1 MTR_3g050390 41.250 240 75 8 31 230 15 228 1.03e-45 152
MS.gene036821.t1 MTR_3g466460 61.176 85 10 2 169 230 10 94 1.26e-23 92.4
MS.gene036821.t1 MTR_8g066730 36.364 110 44 1 54 137 91 200 1.42e-17 79.7
MS.gene036821.t1 MTR_8g066730 36.364 110 44 1 54 137 91 200 1.88e-17 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036821.t1 AT3G19360 34.228 298 93 8 31 230 81 373 1.77e-45 155
MS.gene036821.t1 AT3G19360 34.228 298 93 8 31 230 81 373 1.77e-45 155
MS.gene036821.t1 AT2G35430 33.166 199 91 9 55 230 71 250 1.85e-19 84.3
MS.gene036821.t1 AT1G32360 33.913 115 45 2 54 137 90 204 3.01e-15 74.3

Find 61 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACTAGCTTCTGCGAATT+TGG 0.155359 8.1:+12688533 None:intergenic
ATGAAGATCCTGCACAATTT+AGG 0.163729 8.1:-12688409 MS.gene036821:CDS
CCAAGTATACGTTTCTTCTT+AGG 0.214369 8.1:+12687411 None:intergenic
TGAAGATCCTGCACAATTTA+GGG 0.278155 8.1:-12688408 MS.gene036821:CDS
AGTCAGATCAATCGTATTTA+CGG 0.310380 8.1:-12687378 MS.gene036821:CDS
GATACAGGATATTGTTCCTT+TGG 0.336419 8.1:-12688347 MS.gene036821:CDS
TCAATCGTATTTACGGTGAT+TGG 0.347052 8.1:-12687371 MS.gene036821:CDS
ATTTGTACTAAGTTCAGATT+TGG 0.359410 8.1:-12688620 MS.gene036821:CDS
CACAATTTAGGGGCACTTAT+CGG 0.370314 8.1:-12688397 MS.gene036821:CDS
AATTGCAGCTTCAGCTTCAA+TGG 0.390999 8.1:+12687503 None:intergenic
AAATCAGAGTTACAAGTTCC+TGG 0.400098 8.1:-12687531 MS.gene036821:intron
GACTTGCAGGGGAGGTGGTA+GGG 0.419543 8.1:+12687460 None:intergenic
TGCAAGTCAACTTGTGGAAA+AGG 0.437938 8.1:-12687445 MS.gene036821:CDS
CCTAAGAAGAAACGTATACT+TGG 0.441257 8.1:-12687411 MS.gene036821:CDS
AGATTTGGTGCTTGTAGAAA+CGG 0.444340 8.1:-12688605 MS.gene036821:CDS
CTGAGGCGGTGACATCTTAG+AGG 0.449778 8.1:+12688772 None:intergenic
TTAGAGGTTGGAATATCATC+TGG 0.452783 8.1:+12688788 None:intergenic
CACTTTGCTCATGGTGAAGA+AGG 0.463092 8.1:-12688314 MS.gene036821:intron
TGTCTCAAGTGGAAGGATAC+AGG 0.464303 8.1:-12688362 MS.gene036821:CDS
ACAAATTGCAACTTTGCTCA+TGG 0.469357 8.1:-12688581 MS.gene036821:CDS
CTGATCTGTTTGATTGAGGA+AGG 0.475163 8.1:+12688666 None:intergenic
CTCATGGGGCTGAAGAGATA+AGG 0.488249 8.1:-12688565 MS.gene036821:CDS
TGTGCAAGAAGTATAGCAAT+GGG 0.488339 8.1:-12688469 MS.gene036821:CDS
CAAATTGCAACTTTGCTCAT+GGG 0.490368 8.1:-12688580 MS.gene036821:CDS
TGACTTGCAGGGGAGGTGGT+AGG 0.490726 8.1:+12687459 None:intergenic
GATTGAGGAAGGGTCATCCT+TGG 0.496966 8.1:+12688677 None:intergenic
AGCTTCTGCGAATTTGGAGG+TGG 0.504943 8.1:+12688539 None:intergenic
CACAAGTTGACTTGCAGGGG+AGG 0.509364 8.1:+12687452 None:intergenic
CCAAATTCGCAGAAGCTAGT+TGG 0.511305 8.1:-12688533 MS.gene036821:CDS
ACTAGCTTCTGCGAATTTGG+AGG 0.524749 8.1:+12688536 None:intergenic
GGAAGGGTCATCCTTGGAGG+AGG 0.531078 8.1:+12688683 None:intergenic
GGCGGTGACATCTTAGAGGT+TGG 0.533133 8.1:+12688776 None:intergenic
AATTTACACTTATCTGCATA+GGG 0.538027 8.1:+12688437 None:intergenic
AACAATGACTTCAAAAGAGA+AGG 0.542813 8.1:-12688734 MS.gene036821:CDS
CTCCCCTGCAAGTCAACTTG+TGG 0.543090 8.1:-12687451 MS.gene036821:CDS
AAGTTGACTTGCAGGGGAGG+TGG 0.548779 8.1:+12687455 None:intergenic
AAATTGCAACTTTGCTCATG+GGG 0.550865 8.1:-12688579 MS.gene036821:CDS
AGGAGGGCATATCAATGCAT+TGG 0.555491 8.1:+12688700 None:intergenic
TGAGGAAGGGTCATCCTTGG+AGG 0.556539 8.1:+12688680 None:intergenic
CATTGATATGCCCTCCTCCA+AGG 0.556864 8.1:-12688694 MS.gene036821:CDS
GAATTTACACTTATCTGCAT+AGG 0.559420 8.1:+12688436 None:intergenic
TTTCCACAAGTTGACTTGCA+GGG 0.560920 8.1:+12687448 None:intergenic
TAAGTGCCCCTAAATTGTGC+AGG 0.568865 8.1:+12688401 None:intergenic
TGGTCTGATCTGTTTGATTG+AGG 0.571695 8.1:+12688662 None:intergenic
CTGTGCAAGAAGTATAGCAA+TGG 0.572239 8.1:-12688470 MS.gene036821:CDS
TCAGCTTCAATGGCTCCACC+AGG 0.575223 8.1:+12687513 None:intergenic
CTAAGAAGAAACGTATACTT+GGG 0.583825 8.1:-12687410 MS.gene036821:CDS
AAGAACTGTCACTTTGCTCA+TGG 0.585017 8.1:-12688323 MS.gene036821:CDS
TTCCACAAGTTGACTTGCAG+GGG 0.588906 8.1:+12687449 None:intergenic
AGACTAAGCTGTGTCTCAAG+TGG 0.589149 8.1:-12688373 MS.gene036821:CDS
ATTGTGCAGGATCTTCATGA+AGG 0.599081 8.1:+12688414 None:intergenic
TAAGCTGTGTCTCAAGTGGA+AGG 0.609632 8.1:-12688369 MS.gene036821:CDS
AAAGTGACAGTTCTTTCCAA+AGG 0.613225 8.1:+12688331 None:intergenic
TGATCTGTTTGATTGAGGAA+GGG 0.618914 8.1:+12688667 None:intergenic
GAAGGGTCATCCTTGGAGGA+GGG 0.622595 8.1:+12688684 None:intergenic
AATATCATCTGGAAAAGCCA+TGG 0.624162 8.1:+12688799 None:intergenic
TTCAATGAAGAGAACTGAGG+CGG 0.637828 8.1:+12688758 None:intergenic
GAAGATCCTGCACAATTTAG+GGG 0.650410 8.1:-12688407 MS.gene036821:CDS
CAAGAAGTATAGCAATGGGG+AGG 0.680526 8.1:-12688465 MS.gene036821:CDS
TCAGAGTTACAAGTTCCTGG+TGG 0.718686 8.1:-12687528 MS.gene036821:intron
GTGCAAGAAGTATAGCAATG+GGG 0.763140 8.1:-12688468 MS.gene036821:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAAGTTTTACATT+TGG - chr8.1:12688155-12688174 MS.gene036821:intron 10.0%
!!! GACAATTTTTTTTAAATATA+AGG + chr8.1:12688476-12688495 None:intergenic 10.0%
!!! TTTTTTTAAATATAAGGTCT+AGG + chr8.1:12688470-12688489 None:intergenic 15.0%
!!! CAATAACTCATTTTGATTAT+TGG - chr8.1:12687868-12687887 MS.gene036821:intron 20.0%
!!! TACTATAATTTCTAGTAGTT+TGG - chr8.1:12688183-12688202 MS.gene036821:intron 20.0%
!!! TTATAACAATTGCAACTTTT+TGG - chr8.1:12688557-12688576 MS.gene036821:CDS 20.0%
! AATGGATGATTACTATTGTA+TGG - chr8.1:12688120-12688139 MS.gene036821:intron 25.0%
! AATTTACACTTATCTGCATA+GGG + chr8.1:12687722-12687741 None:intergenic 25.0%
! AGTAATCATCCATTAGAATA+AGG + chr8.1:12688114-12688133 None:intergenic 25.0%
! ATTTGTACTAAGTTCAGATT+TGG - chr8.1:12687536-12687555 MS.gene036821:intron 25.0%
!! ACTTTAAGAAGAAACAAGTT+TGG + chr8.1:12688258-12688277 None:intergenic 25.0%
!! TTAAAGTTTCTGCTGATTAT+TGG - chr8.1:12688271-12688290 MS.gene036821:intron 25.0%
!! TTCGTGTTTTGTAGAAAATA+TGG + chr8.1:12687517-12687536 None:intergenic 25.0%
!!! CTCATTTTGATTATTGGTTT+TGG - chr8.1:12687874-12687893 MS.gene036821:intron 25.0%
AACAATGACTTCAAAAGAGA+AGG - chr8.1:12687422-12687441 MS.gene036821:CDS 30.0%
AAGAGTCAGAACAATATACA+TGG + chr8.1:12688586-12688605 None:intergenic 30.0%
AGTCAGATCAATCGTATTTA+CGG - chr8.1:12688778-12688797 MS.gene036821:CDS 30.0%
ATCATCCATTAGAATAAGGA+TGG + chr8.1:12688110-12688129 None:intergenic 30.0%
CCTCAAAATTATTGATGAGT+CGG + chr8.1:12688312-12688331 None:intergenic 30.0%
CTAAGAAGAAACGTATACTT+GGG - chr8.1:12688746-12688765 MS.gene036821:CDS 30.0%
GAATTTACACTTATCTGCAT+AGG + chr8.1:12687723-12687742 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG + chr8.1:12688231-12688250 None:intergenic 30.0%
TGATTCCATCCTTATTCTAA+TGG - chr8.1:12688102-12688121 MS.gene036821:intron 30.0%
!! TTTTTCAATGAAGAGAACTG+AGG + chr8.1:12687404-12687423 None:intergenic 30.0%
!!! AGGGTTTGTTGAATTTTCTA+TGG + chr8.1:12688680-12688699 None:intergenic 30.0%
!!! TTTGAATGTGTGTCATTTTG+TGG - chr8.1:12688022-12688041 MS.gene036821:intron 30.0%
AAAGTGACAGTTCTTTCCAA+AGG + chr8.1:12687828-12687847 None:intergenic 35.0%
AAATCAGAGTTACAAGTTCC+TGG - chr8.1:12688625-12688644 MS.gene036821:CDS 35.0%
AAATTGCAACTTTGCTCATG+GGG - chr8.1:12687577-12687596 MS.gene036821:intron 35.0%
ACAAATTGCAACTTTGCTCA+TGG - chr8.1:12687575-12687594 MS.gene036821:intron 35.0%
ATCATACACTTGCACACAAA+TGG + chr8.1:12688498-12688517 None:intergenic 35.0%
ATGAAGATCCTGCACAATTT+AGG - chr8.1:12687747-12687766 MS.gene036821:intron 35.0%
CAAATTGCAACTTTGCTCAT+GGG - chr8.1:12687576-12687595 MS.gene036821:intron 35.0%
CCAAGTATACGTTTCTTCTT+AGG + chr8.1:12688748-12688767 None:intergenic 35.0%
CCTAAGAAGAAACGTATACT+TGG - chr8.1:12688745-12688764 MS.gene036821:CDS 35.0%
CTTAATACCACGGTCAAATA+AGG - chr8.1:12688378-12688397 MS.gene036821:CDS 35.0%
CTTATTTGACCGTGGTATTA+AGG + chr8.1:12688380-12688399 None:intergenic 35.0%
GATACAGGATATTGTTCCTT+TGG - chr8.1:12687809-12687828 MS.gene036821:intron 35.0%
TCAATCGTATTTACGGTGAT+TGG - chr8.1:12688785-12688804 MS.gene036821:CDS 35.0%
TGAAGATCCTGCACAATTTA+GGG - chr8.1:12687748-12687767 MS.gene036821:intron 35.0%
TGATCTGTTTGATTGAGGAA+GGG + chr8.1:12687492-12687511 None:intergenic 35.0%
TGTCTTCATCATCACAAATG+CGG + chr8.1:12688413-12688432 None:intergenic 35.0%
TGTTCTGACTCTTGAAGTAT+TGG - chr8.1:12688593-12688612 MS.gene036821:CDS 35.0%
TTAGAGGTTGGAATATCATC+TGG + chr8.1:12687371-12687390 None:intergenic 35.0%
! CCGACTCATCAATAATTTTG+AGG - chr8.1:12688309-12688328 MS.gene036821:intron 35.0%
! TGTGCAAGAAGTATAGCAAT+GGG - chr8.1:12687687-12687706 MS.gene036821:intron 35.0%
!! AGATTTGGTGCTTGTAGAAA+CGG - chr8.1:12687551-12687570 MS.gene036821:intron 35.0%
!! TTGACAAAGCAAGTTCTGAA+TGG - chr8.1:12688075-12688094 MS.gene036821:intron 35.0%
AATTGCAGCTTCAGCTTCAA+TGG + chr8.1:12688656-12688675 None:intergenic 40.0%
ATTGTGCAGGATCTTCATGA+AGG + chr8.1:12687745-12687764 None:intergenic 40.0%
CTGATCTGTTTGATTGAGGA+AGG + chr8.1:12687493-12687512 None:intergenic 40.0%
GAAGATCCTGCACAATTTAG+GGG - chr8.1:12687749-12687768 MS.gene036821:intron 40.0%
TGCAAGTCAACTTGTGGAAA+AGG - chr8.1:12688711-12688730 MS.gene036821:CDS 40.0%
TGGTCTGATCTGTTTGATTG+AGG + chr8.1:12687497-12687516 None:intergenic 40.0%
TTCAATGAAGAGAACTGAGG+CGG + chr8.1:12687401-12687420 None:intergenic 40.0%
! CTGTGCAAGAAGTATAGCAA+TGG - chr8.1:12687686-12687705 MS.gene036821:intron 40.0%
! GCATTAACCTTATTTGACCG+TGG + chr8.1:12688388-12688407 None:intergenic 40.0%
! GTGCAAGAAGTATAGCAATG+GGG - chr8.1:12687688-12687707 MS.gene036821:intron 40.0%
!! AAGAACTGTCACTTTGCTCA+TGG - chr8.1:12687833-12687852 MS.gene036821:intron 40.0%
!! CACAATTTAGGGGCACTTAT+CGG - chr8.1:12687759-12687778 MS.gene036821:intron 40.0%
!! TTTCCACAAGTTGACTTGCA+GGG + chr8.1:12688711-12688730 None:intergenic 40.0%
!!! TTTTCCACAAGTTGACTTGC+AGG + chr8.1:12688712-12688731 None:intergenic 40.0%
AGACTAAGCTGTGTCTCAAG+TGG - chr8.1:12687783-12687802 MS.gene036821:intron 45.0%
CACTTTGCTCATGGTGAAGA+AGG - chr8.1:12687842-12687861 MS.gene036821:intron 45.0%
CCAAATTCGCAGAAGCTAGT+TGG - chr8.1:12687623-12687642 MS.gene036821:intron 45.0%
TAAGCTGTGTCTCAAGTGGA+AGG - chr8.1:12687787-12687806 MS.gene036821:intron 45.0%
TAAGTGCCCCTAAATTGTGC+AGG + chr8.1:12687758-12687777 None:intergenic 45.0%
TCAGAGTTACAAGTTCCTGG+TGG - chr8.1:12688628-12688647 MS.gene036821:CDS 45.0%
TGCCACGATCCTTAATACCA+CGG - chr8.1:12688368-12688387 MS.gene036821:CDS 45.0%
TGGTATTAAGGATCGTGGCA+TGG + chr8.1:12688368-12688387 None:intergenic 45.0%
TGTCTCAAGTGGAAGGATAC+AGG - chr8.1:12687794-12687813 MS.gene036821:intron 45.0%
! ACTAGCTTCTGCGAATTTGG+AGG + chr8.1:12687623-12687642 None:intergenic 45.0%
! AGGAGGGCATATCAATGCAT+TGG + chr8.1:12687459-12687478 None:intergenic 45.0%
! CAAGAAGTATAGCAATGGGG+AGG - chr8.1:12687691-12687710 MS.gene036821:intron 45.0%
! CCAACTAGCTTCTGCGAATT+TGG + chr8.1:12687626-12687645 None:intergenic 45.0%
!! TTCCACAAGTTGACTTGCAG+GGG + chr8.1:12688710-12688729 None:intergenic 45.0%
AGCTTCTGCGAATTTGGAGG+TGG + chr8.1:12687620-12687639 None:intergenic 50.0%
CATTGATATGCCCTCCTCCA+AGG - chr8.1:12687462-12687481 MS.gene036821:CDS 50.0%
CTCATGGGGCTGAAGAGATA+AGG - chr8.1:12687591-12687610 MS.gene036821:intron 50.0%
GACCGTGGTATTAAGGATCG+TGG + chr8.1:12688373-12688392 None:intergenic 50.0%
GATTGAGGAAGGGTCATCCT+TGG + chr8.1:12687482-12687501 None:intergenic 50.0%
CTCCCCTGCAAGTCAACTTG+TGG - chr8.1:12688705-12688724 MS.gene036821:CDS 55.0%
CTGAGGCGGTGACATCTTAG+AGG + chr8.1:12687387-12687406 None:intergenic 55.0%
GAAGGGTCATCCTTGGAGGA+GGG + chr8.1:12687475-12687494 None:intergenic 55.0%
GGCGGTGACATCTTAGAGGT+TGG + chr8.1:12687383-12687402 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG + chr8.1:12688646-12688665 None:intergenic 55.0%
TGAGGAAGGGTCATCCTTGG+AGG + chr8.1:12687479-12687498 None:intergenic 55.0%
! AAGGTCTAGGACCACGACTG+TGG + chr8.1:12688457-12688476 None:intergenic 55.0%
!! AAGTTGACTTGCAGGGGAGG+TGG + chr8.1:12688704-12688723 None:intergenic 55.0%
!! CACAAGTTGACTTGCAGGGG+AGG + chr8.1:12688707-12688726 None:intergenic 55.0%
GACTTGCAGGGGAGGTGGTA+GGG + chr8.1:12688699-12688718 None:intergenic 60.0%
GGAAGGGTCATCCTTGGAGG+AGG + chr8.1:12687476-12687495 None:intergenic 60.0%
GTACGTCTCAGCCACAGTCG+TGG - chr8.1:12688443-12688462 MS.gene036821:CDS 60.0%
! TGACTTGCAGGGGAGGTGGT+AGG + chr8.1:12688700-12688719 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 12687359 12688819 12687359 ID=MS.gene036821
chr8.1 mRNA 12687359 12688819 12687359 ID=MS.gene036821.t1;Parent=MS.gene036821
chr8.1 exon 12688315 12688819 12688315 ID=MS.gene036821.t1.exon1;Parent=MS.gene036821.t1
chr8.1 CDS 12688315 12688819 12688315 ID=cds.MS.gene036821.t1;Parent=MS.gene036821.t1
chr8.1 exon 12687359 12687546 12687359 ID=MS.gene036821.t1.exon2;Parent=MS.gene036821.t1
chr8.1 CDS 12687359 12687546 12687359 ID=cds.MS.gene036821.t1;Parent=MS.gene036821.t1
Gene Sequence

>MS.gene036821

ATGGCTTTTCCAGATGATATTCCAACCTCTAAGATGTCACCGCCTCAGTTCTCTTCATTGAAAAACAATGACTTCAAAAGAGAAGGAACTTCTCATTCCAATGCATTGATATGCCCTCCTCCAAGGATGACCCTTCCTCAATCAAACAGATCAGACCATATTTTCTACAAAACACGAATTTGTACTAAGTTCAGATTTGGTGCTTGTAGAAACGGAACAAATTGCAACTTTGCTCATGGGGCTGAAGAGATAAGGAAACCACCTCCAAATTCGCAGAAGCTAGTTGGTCTGTGCAACGAAGATAAAAAAATCATAAACAAGATGAAACTGTGCAAGAAGTATAGCAATGGGGAGGAATGTCCCTATGCAGATAAGTGTAAATTCCTTCATGAAGATCCTGCACAATTTAGGGGCACTTATCGGAAGACTAAGCTGTGTCTCAAGTGGAAGGATACAGGATATTGTTCCTTTGGAAAGAACTGTCACTTTGCTCATGGTGAAGAAGGTAACAATAACTCATTTTGATTATTGGTTTTGGTTTTATCATATAATTCTACTGTTTTATATTGTTCAATCATATAAAATATGTTTTTTTAATTTTCTAGATGCTGTGCAGAATCTCATTTTATGTGATATGATGTGCAAATTACAACGATTAAATGATTTGAATGTGTGTCATTTTGTGGTTGCGTTGTGTATTATGTTTCATTTTTTATTTGACAAAGCAAGTTCTGAATGGAAAATGATTCCATCCTTATTCTAATGGATGATTACTATTGTATGGTGTTAAAAGTTAAAAAAAAAAAGTTTTACATTTGGTAAGATACTATAATTTCTAGTAGTTTGGAGTAAGTTGCGATTCTTAATAACCAGCTATTGTATTATGTTCATCTTTTCCAAACTTGTTTCTTCTTAAAGTTTCTGCTGATTATTGGCTATAAATTTGATGTCCGACTCATCAATAATTTTGAGGTGCATTGCTCATTTGCAAAGTTTTAAATTGTGACCATGCCACGATCCTTAATACCACGGTCAAATAAGGTTAATGCAGCCGCATTTGTGATGATGAAGACATAAGACATTTGTACGTCTCAGCCACAGTCGTGGTCCTAGACCTTATATTTAAAAAAAATTGTCCATTTGTGTGCAAGTGTATGATAACTTATATTTGCATATTGTGAGCAAAATTACAAGATGATTATAACAATTGCAACTTTTTGGTTTCCATGTATATTGTTCTGACTCTTGAAGTATTGGTTGTCTTGCAAATCAGAGTTACAAGTTCCTGGTGGAGCCATTGAAGCTGAAGCTGCAATTACCATAGAAAATTCAACAAACCCTACCACCTCCCCTGCAAGTCAACTTGTGGAAAAGGAAGAAGAGCAGCCTAAGAAGAAACGTATACTTGGGTTGAAATTCAGTCAGATCAATCGTATTTACGGTGATTGGATTGACGATTGA
Protein sequence

>MS.gene036821.t1

MAFPDDIPTSKMSPPQFSSLKNNDFKREGTSHSNALICPPPRMTLPQSNRSDHIFYKTRICTKFRFGACRNGTNCNFAHGAEEIRKPPPNSQKLVGLCNEDKKIINKMKLCKKYSNGEECPYADKCKFLHEDPAQFRGTYRKTKLCLKWKDTGYCSFGKNCHFAHGEEELQVPGGAIEAEAAITIENSTNPTTSPASQLVEKEEEQPKKKRILGLKFSQINRIYGDWIDD