AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036932

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036932.t1 MTR_7g073990 98.907 183 2 0 1 183 63 245 2.45e-133 373
MS.gene036932.t1 MTR_1g018030 44.295 149 72 3 35 180 44 184 5.19e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036932.t1 AT1G32070 71.354 192 43 2 3 182 65 256 9.52e-91 266
MS.gene036932.t1 AT1G32070 70.466 193 44 2 3 182 64 256 9.88e-90 263
MS.gene036932.t1 AT1G32070 70.466 193 44 2 3 182 65 257 1.02e-89 263
MS.gene036932.t1 AT1G26220 41.353 133 67 2 49 180 67 189 1.07e-27 103

Find 44 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAACTTCCTTATCAATT+TGG 0.118919 7.3:+31229282 None:intergenic
ATGCCTTCAATGCCACAATT+TGG 0.217116 7.3:-31230540 MS.gene036932:CDS
AATGAACAAAAGATATTAAT+TGG 0.255156 7.3:-31230586 MS.gene036932:CDS
GAGCAAATATTGTTCTCTTC+AGG 0.294940 7.3:-31236050 MS.gene036932:CDS
GAAAACTTCCTTATCAATTT+GGG 0.297922 7.3:+31229283 None:intergenic
GCTGACCCAGAGGGCATAAA+AGG 0.303809 7.3:-31229318 MS.gene036932:CDS
TGCCTTCAATGCCACAATTT+GGG 0.345095 7.3:-31230539 MS.gene036932:CDS
ATTCTATAGGGCCAAGGTCT+TGG 0.389671 7.3:-31229556 MS.gene036932:intron
GCAGCAGCTAATTTCGATAA+TGG 0.399700 7.3:+31230809 None:intergenic
ATTTGCTCAATTGTTCCATC+AGG 0.431467 7.3:+31236065 None:intergenic
AGGGCATAAAAGGCATGTTC+TGG 0.444015 7.3:-31229308 MS.gene036932:CDS
ATCCCAAATTGTGGCATTGA+AGG 0.446021 7.3:+31230537 None:intergenic
TGCCAAAGTACCTGGTAGCC+TGG 0.451629 7.3:+31230502 None:intergenic
CCTAGTTGATCCAGGCTACC+AGG 0.486692 7.3:-31230512 MS.gene036932:intron
CACCACACAGGTCATCGATC+TGG 0.494760 7.3:-31236153 MS.gene036932:CDS
ATAAGAAAGTCACCAGGGTC+AGG 0.499788 7.3:-31230749 MS.gene036932:intron
TGGGATGTCCTAGTTGATCC+AGG 0.505992 7.3:-31230520 MS.gene036932:CDS
CAAATATTGTTCTCTTCAGG+TGG 0.543956 7.3:-31236047 MS.gene036932:CDS
GATTTGATTCTATAGGGCCA+AGG 0.548964 7.3:-31229562 MS.gene036932:intron
CAAGAGCTTTACCAAGACCT+TGG 0.549605 7.3:+31229545 None:intergenic
AGCACAAACTCTTGAGGTAG+AGG 0.550148 7.3:+31236104 None:intergenic
CCTGGTAGCCTGGATCAACT+AGG 0.553781 7.3:+31230512 None:intergenic
ACAAAAGATATTAATTGGCA+TGG 0.555166 7.3:-31230581 MS.gene036932:CDS
AAATTGTGGCATTGAAGGCA+TGG 0.561363 7.3:+31230542 None:intergenic
GGCATGGTCTGATGTAGCAC+GGG 0.584570 7.3:+31230558 None:intergenic
GGGTTTGAAGCTGACCCAGA+GGG 0.584967 7.3:-31229327 MS.gene036932:CDS
GATACGCACCTTGTCACAGA+GGG 0.585718 7.3:+31235998 None:intergenic
TCAACAAGCACAAACTCTTG+AGG 0.586333 7.3:+31236098 None:intergenic
ACTTTGTTCGCAGATAGTAA+AGG 0.599684 7.3:-31229475 MS.gene036932:intron
AACTAGGACATCCCAAATTG+TGG 0.601622 7.3:+31230528 None:intergenic
TCTTCAAGCCCTCTGTGACA+AGG 0.604383 7.3:-31236006 MS.gene036932:intron
ATATTATTATTACCTGACCC+TGG 0.611994 7.3:+31230737 None:intergenic
AGGCATGGTCTGATGTAGCA+CGG 0.613503 7.3:+31230557 None:intergenic
GTTGAAAAGACTGAACCTGA+TGG 0.616747 7.3:-31236080 MS.gene036932:CDS
TATTCTATAAGAAAGTCACC+AGG 0.624420 7.3:-31230755 MS.gene036932:CDS
CATCTCTTTAACAGGTAGGG+TGG 0.637083 7.3:-31230841 MS.gene036932:intron
AACAGGTAGGGTGGCCTCGT+AGG 0.640562 7.3:-31230832 MS.gene036932:intron
AATTTCGATAATGGCCTACG+AGG 0.672834 7.3:+31230818 None:intergenic
AGGGTTTGAAGCTGACCCAG+AGG 0.695850 7.3:-31229328 MS.gene036932:CDS
ATTCTATAAGAAAGTCACCA+GGG 0.724149 7.3:-31230754 MS.gene036932:CDS
GATGAAGAATAACACCACAC+AGG 0.731975 7.3:-31236165 MS.gene036932:CDS
AGATACGCACCTTGTCACAG+AGG 0.742856 7.3:+31235997 None:intergenic
CTTTCTTATAGAATACAGAG+AGG 0.746297 7.3:+31230763 None:intergenic
CTCCAGATCGATGACCTGTG+TGG 0.755205 7.3:+31236151 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.3 gene 31229296 31236186 31229296 ID=MS.gene036932
chr7.3 mRNA 31229296 31236186 31229296 ID=MS.gene036932.t1;Parent=MS.gene036932
chr7.3 exon 31236007 31236186 31236007 ID=MS.gene036932.t1.exon1;Parent=MS.gene036932.t1
chr7.3 CDS 31236007 31236186 31236007 ID=cds.MS.gene036932.t1;Parent=MS.gene036932.t1
chr7.3 exon 31230750 31230849 31230750 ID=MS.gene036932.t1.exon2;Parent=MS.gene036932.t1
chr7.3 CDS 31230750 31230849 31230750 ID=cds.MS.gene036932.t1;Parent=MS.gene036932.t1
chr7.3 exon 31230513 31230613 31230513 ID=MS.gene036932.t1.exon3;Parent=MS.gene036932.t1
chr7.3 CDS 31230513 31230613 31230513 ID=cds.MS.gene036932.t1;Parent=MS.gene036932.t1
chr7.3 exon 31229476 31229569 31229476 ID=MS.gene036932.t1.exon4;Parent=MS.gene036932.t1
chr7.3 CDS 31229476 31229569 31229476 ID=cds.MS.gene036932.t1;Parent=MS.gene036932.t1
chr7.3 exon 31229296 31229372 31229296 ID=MS.gene036932.t1.exon5;Parent=MS.gene036932.t1
chr7.3 CDS 31229296 31229372 31229296 ID=cds.MS.gene036932.t1;Parent=MS.gene036932.t1
Gene Sequence

>MS.gene036932

ATGAAGAATAACACCACACAGGTCATCGATCTGGAGTTAACAGAAGAAGAAGACGAAGAACCTCTACCTCAAGAGTTTGTGCTTGTTGAAAAGACTGAACCTGATGGAACAATTGAGCAAATATTGTTCTCTTCAGGTGGAGATCTTGATGTTTATGATCTTCAAGCCCTCTGTGACAAGGTGCGTATCTAATATGTTTTTATGATGTATATTGATTGTTGCGAATTCGATGAAAAACTCACACCAATAACAAACTTGATGTGTGGAGCAAGGGATAATTAAGCAACCAAAATCAAAGTGTATGGAACAGTTATAATCACAAGCCAAAAGTAGAAAACAACAGCGAGAGAAAAGGAAGAGAGAACACAAAGAATTTGTTGACCCAGTTCGGTCCAAATTGACCTAGTCTGGGGGAGAGAGCAGCCCTCCGTTCCACTATATGATGAGTAACGTTACAAAAGAGATTTCAATGGGTTACAAGAATAAGTCTCCCGCCTAATTCTACCCAAATCCCAGCCTCTTCCCGTAACCAAGAGACTCAATCCTAATAGTGTTTCCCAAGGTGAATCAACCTTCAAACCCTAGTTTGTTGCCAAGAGACAAACTCTATACAAACCCTAGTTTGATTTCTAAACCCTTGTTTCAGCCCCCTCTATCTCTCTCAAAATAGTCTGATACAAGGCCTATATTTATAGTAAAAGAAACTCCCATAAAATTGTAACAAATCTGGCCCAAAAATACGTTTCTGAAAAATAACAGCACGCGAAATCGGCCACCGTGCTCTGAAAATCGGCCACCGATTTTTCCCGAACAGAAAGCCTCCCAAAAGGCAGCCAAAATCGGCCACCGATTTTCCAAATCGGCCACCGATTTGTACTTTCAGCAAACATCAATTTTGGCCTCCAAACTCCAATTTTGAGTCACTTTCACAACAATCTCCACCTTGACTCCAAATTAGTGATGATGCCCATTTAACATCAACACCTTGCTGCTCTTTCCCTTGCTTCCCACTACACCAAGTAGCTCCACAAGTTTACACCCTAATCCCTTCAGCCATCGGAATGTCTTCCGCCTTCTCGAAGGTACTTGCAGTCCAACTACGCCAGGAATTTTAGGTAGTTCCACAAGCCTTTACCATACCTCTTCAATACGAGACATCTCCCGCTTCCTTGAAGATCTACTTGTTCCCAATCACCCTTGGCTTTTAGAGTAATCCACAAGTAAACACCTCCCATCCCTTCAGCCCTCGGAATGTCTTCCGCCTTCTCGAAGGTCCTTGCAGTCTAACTACGCTAGGTATTTTAGGTAGTCCCACAAGCATTCACCATGACATCTTCAACACAGGACATCTCCCGCTTCCTTGAAGGTCTCCTTGCATCCAATCACCCTTGGCCTTTTTTAGGGTAATCCCGCAAGCTGTGCATTACATTCTTCAACCTTATGAAGAAATCTCTTGCTCACCATAGAGCATAGCACCCAAGGTCCTCCATAGCTGTACCATTACCGGTGTGTTCAACTCCACCTCAAGCTGAGCGTACTCTACCTCAACTAGCAAATGCGTACATCCCACTATGTCTTCAACCTTGAAACTCCACCTCAACAGGTAGATCATGAGTATTCTTACCACCGCCTCTCCAGGCTGATGTTGAGTGTTTAACGTCTCTCCAGACGACCACCGCTCCACTAGGCATCAATACCAAGGTGACCAACATCACCTTCATCCTCCAAACAACACCACACCATCAGAGCACCCGCATCCCCAAGACGCACCTCAATGTTCTCCAAATCACCAATGATTTTGTTGAACTCCACAAGTAGCTCCATGACGGCCTTTAACTTTACCATCCTGAATAAGTAAAGTTGTTGCTTTAGGAATTGCCGACGAGCCAAAGACTTTGTCATATACAAATATTCGAACTTTGACCACATCGATGCCGCGGTTGTATCCTTTGTTGCTTCTCTCAAAACTTTATCTCCGAGGCACAAGACAATGGCACTCCTGGCCTTGTCCACCATCTCGGTCTTCTCCGCTCGTGTCATGGTGACCGGTAACGAACCTTCACCCTTCAAAGCTTTCTCACACTTTTGTTGTATCAACACCGCTTCCATCTTTACCTTCCATAACCCAAAATCGTTATCGCCGGTAAACTTCTCAATATCCCACTTCGAACCCATGGTACTTGATTATCCTTTGACCTTTGCCCCACGGTGGGCGCCAATTGTTGCGAATTCGATGAAAAACTCACACCAATAACAAACTTGATGTGTGGAGCAAGGGATAATTAAGCAACCAAAATCAAAGTGTATGGAACAGTTATAATCACAAGCCAAAAGTAGAAAACAACAGCGAGAGAAAAGGAAGAGAGAACACAAAGAATTTGTTGACCCAGTTCGGTCCAAATTGACCTAGTCTGGGGGAGAGAGCAGCCCTCCGTTCCACTATATGATGAGTAACGTTACAAAAGAGATTTCAATGGGTTACAAGAATAAGTCTCCCGCCTAATTCTACCCAAATCCCAGCCTCTTCCCGTAACCAAGAGACTCAATCCTAATAGTGTTTCCCAAGGTGAATCAACCTTCAAACCCTAGTTTGTTGCCAAGAGACAAACTCTATACAAACCCTAGTTTGATTTCTAAACCCTTGTTTCAGCCCCCTCTATCTCTCTCAAAATAGTCTGATACAAGGCCTATATTTATAGTAAAAGAAACTCCCATAAAATTGTAACAAATCTGGCCCAAAAATACGTTTCTGAAAAATAACAGCACGCGAAATCGGCCACCGTGCTCTGAAAATCGGCCACCGATTTTTCCCGAACAGAAAGCCTCCCAAAAGGCAGCCAAAATCGGCCACCGATTTTCCAAATCGGCCACCGATTTGTACTTTCAGCAAACATCAATTTTGGCCTCCAAACTCCAATTTTGAGTCACTTTCACAACAATCTCCACCTTGACTCCAAATTAGTGATGATGCCCATTTAACATCAACACCTTGCTGCTCTTTCCCTTGCTTCCCACTACACCAAGTAGCTCCACAAGTTTACACCCTAATCCCTTCAGCCATCGGAATGTCTTCCGCCTTCTCGAAGGTACTTGCAGTCCAACTACGCCAGGAATTTTAGGTAGTTCCACAAGCCTTTACCATACCTCTTCAATACGAGACATCTCCCGCTTCCTTGAAGATCTACTTGTTCCCAATCACCCTTGGCTTTTAGAGTAATCCACAAGTAAACACCTCCCATCCCTTCAGCCCTCGGAATGTCTTCCGCCTTCTCGAAGGTCCTTGCAGTCTAACTACGCTAGGTATTTTAGGTAGTCCCACAAGCATTCACCATGACATCTTCAACACAGGACATCTCCCGCTTCCTTGAAGGTCTCCTTGCATCCAATCACCCTTGGCCTTTTTTAGGGTAATCCCGCAAGCTGTGCATTACATTCTTCAACCTTATGAAGAAATCTCTTGCTCACCATAGAGCATAGCACCCAAGGTCCTCCATAGCTGTACCATTACCGGTGTGTTCAACTCCACCTCAAGCTGAGCGTACTCTACCTCAACTAGCAAATGCGTACATCCCACTATGTCTTCAACCTTGAAACTCCACCTCAACAGGTAGATCATGAGTATTCTTACCACCGCCTCTCCAGGCTGATGTTGAGTGTTTAACGTCTCTCCAGACGACCACCGCTCCACTAGGCATCAATACCAAGGTGACCAACATCACCTTCATCCTCCAAACAACACCACACCATCAGAGCACCCGCATCCCCAAGACGCACCTCAATGTTCTCCAAATCACCAATGATTTTGTTGAACTCCACAAGTAGCTCCATGACGGCCTTTAACTTTACCATCCTGAATAAGTAAAGTTGTTGCTTTAGGAATTGCCGACGAGCCAAAGACTTTGTCATATACAAATATTCGAACTTTGACCACATCGATGCCGCGGTTGTATCCTTTGTTGCTTCTCTCAAAACTTTATCTCCGAGGCACAAGACAATGGCACTCCTGGCCTTGTCCACCATCTCGGTCTTCTCCGCTCGTGTCATGGTGACCGGTAACGAACCTTCACCCTTCAAAGCTTTCTCACACTTTTGTTGTATCAACACCGCTTCCATCTTTACCTTCCATAACCCAAAATCGTTATCGCCGGTAAACTTCTCAATATCCCACTTCGAACCCATGGTACTTGATTATCCTTTGACCTTTGCCCCACGGTGGGCGCCAATTGTTGCGAATTCGATGAAAAACTCACACCAATAACAAACTTGATGTGTGGAGCAAGGGATAATTAAGCAACCAAAATCAAAGTGTATGGAACAGTTATAATCACAAGCCAAAAGTAGAAAACAACAGCGAGAGAAAAGGAAGAGAGAACACAAAGAATTTGTTGACCCAGTTCGGTCCAAATTGACCTAGTCTGGGGGAGAGAGCAGCCCTCCGTTCCACTATATGATGAGTAACGTTACAAAAGAGATTTCAATGGGTTACAAGAATAAGTCTCCCGCCTAATTCTACCCAAATCCCAGCCTCTTCCCGTAACCAAGAGACTCAATCCTAATAGTGTTTCCCAAGGTGAATCAACCTTCAAACCCTAGTTTGTTGCCAAGAGACAAACTCTATACAAACCCTAGTTTGATTTCTAAACCCTTGTTTCAGCCCCCTCTATCTCTCTCAAAATAGTCTGATACAAGGCCTATATTTATAGTAAAAGAAACTCCCATAAAATTGTAACAAATCTGGCCCAAAAATACGTTTCTGAAAAATAACAGCACGCGAAATCGGCCACCGTGCTCTGAAAATCGGCCACCGATTTTTCCCGAACAGAAAGCCTCCCAAAAGGCAGCCAAAATCGGCCACCGATTTGTACTTTCAGCAAACATCAATTTTGGCCTCCAAACTCCAATTTTGAGTCACTTTCACAACATTGATGTTTTTGTTGAAACTGCAAGACCGTGATTATGTGTATGCTAGTTTTTTTTCAAATTACTTTTCAGACACAAAACCATGTGCGTGCACTCAATTCATATTTGAGGAACTTGTGTGCGACCACATTAAGAACTTATTTCTTCCTTTCATTTTTGTATCTATATTAATCAATATCTTTATCATTATTTTAGTTCCTTAGCAACATTAAGAACTCAATTATTTATGTACATTAATCCTCACAAGAGTTTCCTTCGGTATAGTTTAATAATTTTCAGTACCTGTGTTGTTTAACATTGTAATTTACAGATTCTGTGTATCATTTATTATCACTAAAACTTAAAGTACTAAAATTTCAAAATTGTTTATTATATGAAACTCGTCCTTGGTTACTAAATAGTTCATCTCTTTAACAGGTAGGGTGGCCTCGTAGGCCATTATCGAAATTAGCTGCTGCTTTGAAAAATAGCTATATGGTGGCCTCTCTGTATTCTATAAGAAAGTCACCAGGGTCAGGTAATAATAATATGACATCTTACTGTGTCTTGTGATTAAGTTAATTCGTTGGATGGTTAAACTTTTTGTTTATATTTGTAGCTGTCTAAACCTATTTGATGAATATCTTAGTGTACTTGGTGAAGTTATTTGACAGAGGGGAATGAACAAAAGATATTAATTGGCATGGCCCGTGCTACATCAGACCATGCCTTCAATGCCACAATTTGGGATGTCCTAGTTGATCCAGGCTACCAGGTACTTTGGCAACCAAACATAGATTTTGAATACAAACTCGGGTGCAAGTTAATGAAAGTGTTTGCTTCTAAAACTAGAATCAAGTTCATGAACAATATAACTTTTAATTTTTTATATTGTATTATTCTTGCTAATTACTAAATTAGAAATGAATAGACGGTGATGCACGTAATCAGATCTCTGTGTTGATTATCTTGCACACTATTGCTCTCATATGTAGCCTAAAGGTTCACTTTTTTAATGGTTTCATGGTTCTGGACCAACACATTTGCTTTAGTAGAGGACCACCATATGTCATATGGCCTTTGGGTTTGTTTAATTTAAATTATCTTTAAGTTATACTCCTAGATGTCATATTAATTTAAAATAATAGCTTGATTCAAACAATTGACTTTTTTATTTCTTTTTTTGAAGAATTGATTCAAACAGTTGTTGAAATGGGAAGCAAATAAATAATTTTAACAGATATACAATATGTTAAGATCTTAAAAATCTTATATCTTGTGTGAATAAATTTGTTCAAAAATACGTTTTACATAAGTATTGGGATTCATTTAGGTGCAAAGAATGATGTTCTTAAGATAAATGTTCAATAAAAATAGATGAGCAGCAAAACATTTATTCCATTCATTTCCTCATTAGAACTTCCATACAAAAGCAGTGTTCTTTTTTGTTTCAAGGCCAGCCCTTTTTGAACCACTCTACTTGATGTTCGTTAGACCCGCCACACCCTCACTTAGGGAAAGTTAAATGTTTCATGCTACAATCTTTGATTATATAGTACAAATTTGAAAAACACATTAGGTATTTTTGGATTCAGGGATACTGCCAAATTTTATCTTTTATGCTTAAGTGCCTCCTTGTGTTCTGTTCCAGTATAAACATAGATTATGGCTTCATATAGACTTGAGTACTGCTTATTGATGTTGATTTGATTCTATAGGGCCAAGGTCTTGGTAAAGCTCTTGTAGAGAAACTCATTCGAGCTCTTTTGCAAAGGGACATCGGTAATATTACTTTGTTCGCAGATAGTAAAGGTATAACAATACTGACAAACTTTTCTTGAATTTCATGCGCTTTCATGGTTTATGTAAAATAGAAATTGGAGTGTAACTAATTGTTATTGTATTTTGAATGCAGTCGTGGAATTTTATCGCAATTTAGGGTTTGAAGCTGACCCAGAGGGCATAAAAGGCATGTTCTGGTACCCAAATTGA
Protein sequence

>MS.gene036932.t1

MKNNTTQVIDLELTEEEDEEPLPQEFVLVEKTEPDGTIEQILFSSGGDLDVYDLQALCDKVGWPRRPLSKLAAALKNSYMVASLYSIRKSPGSEGNEQKILIGMARATSDHAFNATIWDVLVDPGYQGQGLGKALVEKLIRALLQRDIGNITLFADSKVVEFYRNLGFEADPEGIKGMFWYPN