Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037211.t1 | RHN45253.1 | 81.5 | 507 | 66 | 3 | 1 | 482 | 1 | 504 | 6.30E-198 | 700.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037211.t1 | Q9S7L2 | 64.0 | 125 | 44 | 1 | 257 | 381 | 217 | 340 | 6.7e-39 | 163.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037211.t1 | A0A396GXN1 | 81.5 | 507 | 66 | 3 | 1 | 482 | 1 | 504 | 4.6e-198 | 700.3 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037211.t1 | MTR_7g035350 | 85.177 | 452 | 63 | 2 | 32 | 482 | 2 | 450 | 0.0 | 780 |
| MS.gene037211.t1 | MTR_7g451170 | 48.764 | 445 | 171 | 7 | 35 | 475 | 5 | 396 | 2.68e-132 | 389 |
| MS.gene037211.t1 | MTR_4g088015 | 48.536 | 478 | 189 | 8 | 1 | 474 | 1 | 425 | 2.55e-124 | 370 |
| MS.gene037211.t1 | MTR_7g037130 | 47.395 | 403 | 149 | 7 | 75 | 475 | 1 | 342 | 2.87e-116 | 346 |
| MS.gene037211.t1 | MTR_7g037260 | 47.264 | 402 | 151 | 7 | 75 | 475 | 1 | 342 | 5.56e-116 | 345 |
| MS.gene037211.t1 | MTR_1g057980 | 53.016 | 315 | 123 | 2 | 159 | 473 | 3 | 292 | 1.38e-107 | 322 |
| MS.gene037211.t1 | MTR_7g450950 | 36.453 | 406 | 175 | 7 | 62 | 465 | 13 | 337 | 1.81e-72 | 233 |
| MS.gene037211.t1 | MTR_3g077650 | 48.039 | 204 | 98 | 2 | 206 | 409 | 137 | 332 | 4.70e-50 | 177 |
| MS.gene037211.t1 | MTR_4g105660 | 52.500 | 160 | 67 | 2 | 227 | 386 | 158 | 308 | 2.86e-46 | 166 |
| MS.gene037211.t1 | MTR_3g463400 | 53.731 | 134 | 58 | 1 | 253 | 382 | 129 | 262 | 1.06e-44 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037211.t1 | AT4G18770 | 64.754 | 122 | 42 | 1 | 260 | 381 | 220 | 340 | 1.02e-47 | 170 |
| MS.gene037211.t1 | AT3G27785 | 43.049 | 223 | 111 | 6 | 170 | 386 | 109 | 321 | 4.90e-47 | 169 |
| MS.gene037211.t1 | AT5G40360 | 47.590 | 166 | 82 | 4 | 225 | 387 | 126 | 289 | 1.80e-42 | 154 |
| MS.gene037211.t1 | AT5G58850 | 44.848 | 165 | 75 | 2 | 254 | 402 | 102 | 266 | 1.37e-40 | 151 |
| MS.gene037211.t1 | AT5G11050 | 57.377 | 122 | 52 | 0 | 244 | 365 | 92 | 213 | 3.42e-40 | 150 |
| MS.gene037211.t1 | AT2G25230 | 52.033 | 123 | 58 | 1 | 260 | 382 | 29 | 150 | 3.85e-36 | 134 |
| MS.gene037211.t1 | AT3G09370 | 61.702 | 94 | 36 | 0 | 260 | 353 | 133 | 226 | 3.23e-35 | 137 |
| MS.gene037211.t1 | AT3G09370 | 61.702 | 94 | 36 | 0 | 260 | 353 | 138 | 231 | 3.49e-35 | 137 |
| MS.gene037211.t1 | AT3G09370 | 61.702 | 94 | 36 | 0 | 260 | 353 | 146 | 239 | 5.29e-35 | 137 |
| MS.gene037211.t1 | AT5G11510 | 55.446 | 101 | 44 | 1 | 260 | 359 | 84 | 184 | 2.14e-34 | 137 |
Find 113 sgRNAs with CRISPR-Local
Find 176 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCAGAGATCCCCGCAA+CGG | 0.643024 | 7.3:+62760037 | MS.gene037211:CDS |
| AAAATCGCTCTGGGATCTAT+TGG | 0.375361 | 7.3:-62760252 | None:intergenic |
| AAAATTTGGGCTCAATAACT+GGG | 0.569139 | 7.3:+62760990 | MS.gene037211:CDS |
| AAATTCCTTTGTTAGTATTA+AGG | 0.254505 | 7.3:-62760454 | None:intergenic |
| AAATTGCAAGTAGATTGCCT+GGG | 0.636725 | 7.3:+62762227 | MS.gene037211:CDS |
| AAATTTCTTTGCCAGTATTA+AGG | 0.278768 | 7.3:-62760340 | None:intergenic |
| AACATGACTAGGACCAGGGT+TGG | 0.597563 | 7.3:-62762461 | None:intergenic |
| AACATTACCAATACCAACCC+TGG | 0.565873 | 7.3:+62762448 | MS.gene037211:CDS |
| AACCAACATGACTAGGACCA+GGG | 0.623604 | 7.3:-62762465 | None:intergenic |
| AACCCTGGTCCTAGTCATGT+TGG | 0.596471 | 7.3:+62762463 | MS.gene037211:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAACTGGTGTAATGTGA+TGG | - | chr7.3:62760098-62760117 | None:intergenic | 30.0% | |
| AAAAATTTGGGCTCAATAAC+TGG | + | chr7.3:62760989-62761008 | MS.gene037211:CDS | 30.0% | |
| AAAATTTGGGCTCAATAACT+GGG | + | chr7.3:62760990-62761009 | MS.gene037211:CDS | 30.0% | |
| ATGTTCCTCTCAAACTATTT+CGG | - | chr7.3:62762327-62762346 | None:intergenic | 30.0% | |
| CAACAAAATCTCTTTCAACA+TGG | + | chr7.3:62760374-62760393 | MS.gene037211:CDS | 30.0% | |
| CATTCTAAGTGTATGTGTAA+GGG | - | chr7.3:62761146-62761165 | None:intergenic | 30.0% | |
| GAAAATTCGTTGATATACGA+AGG | - | chr7.3:62760197-62760216 | None:intergenic | 30.0% | |
| GAGATTGCTAAGTTTATGAA+AGG | + | chr7.3:62761015-62761034 | MS.gene037211:CDS | 30.0% | |
| GCTTTGATTGTACAAAAAAC+TGG | - | chr7.3:62760111-62760130 | None:intergenic | 30.0% | |
| GTAAGGGATAAAAGCTAAAA+AGG | - | chr7.3:62761130-62761149 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 62759966 | 62762678 | 62759966 | ID=MS.gene037211 |
| chr7.3 | mRNA | 62759966 | 62762678 | 62759966 | ID=MS.gene037211.t1;Parent=MS.gene037211 |
| chr7.3 | exon | 62759966 | 62760762 | 62759966 | ID=MS.gene037211.t1.exon1;Parent=MS.gene037211.t1 |
| chr7.3 | CDS | 62759966 | 62760762 | 62759966 | ID=cds.MS.gene037211.t1;Parent=MS.gene037211.t1 |
| chr7.3 | exon | 62760969 | 62761095 | 62760969 | ID=MS.gene037211.t1.exon2;Parent=MS.gene037211.t1 |
| chr7.3 | CDS | 62760969 | 62761095 | 62760969 | ID=cds.MS.gene037211.t1;Parent=MS.gene037211.t1 |
| chr7.3 | exon | 62762154 | 62762678 | 62762154 | ID=MS.gene037211.t1.exon3;Parent=MS.gene037211.t1 |
| chr7.3 | CDS | 62762154 | 62762678 | 62762154 | ID=cds.MS.gene037211.t1;Parent=MS.gene037211.t1 |
| Gene Sequence |
>MS.gene037211 ATGGAATTAGATCCTAGTTTTTCAGAAAAAATTTCTTATCTCTTAGGAGATTTTCCTAAAATTCCTCAAGAAAAATCAGAGATCCCCGCAACGGTTCTCTTGCAACCAAGTTCTACTATTTCACCTACACCATCACATTACACCAGTTTTTTGTACAATCAAAGCAGTCAATTCAATGAACATCCTCTGAACATGGCTAACCAAGACTTTAGGGTTTCTACTGTAGTTCCTTCGTATATCAACGAATTTTCTATGATCCCAACCCCTTCATACAGGGGTGCAAACACCAATAGATCCCAGAGCGATTTTTTCAACGACCAAATTCCAATAACCTTTACACCAAACAATAACAAAAATGAATTTATGCATGAACACCTTAATACTGGCAAAGAAATTTGGGATTTTTCCCAACAAAATCTCTTTCAACATGGTCAAACTTCACAATCTCATTTCAGTCCCTCTCTATCAACATCAGTAGTTTATGAACGCCTTAATACTAACAAAGGAATTTGGGATTTCTCCCAACAAAATCTCTTTCAATATGGTGAAACTTCACAATCTCAGGTCAGTCCTTCTCTACCAACATCAGTAGTTTATGATGAAAACCCATCTTTGTTAGTGCCTAGGCTTCAAGCCAATATTTCTTGCATTGGTGGGTTTGAAAACAATACCCAACACAATGATGAAGGACTAATCCCCAACAACCAAAAGCGTCAAAAAAGGGTTGATAAGACTATTGATATTCAACAAAGGGATTTCAATACAAGCAAAAGGCTATGGACTTCAGATGAGGATAGGTATATCAAATCATTTAAAGTTTGTACTTTCTCTGCTATCCAATATTTTCATGGTTTTAGTTTGGCTTCGTTATATTCTTTATTCAAGTTATGTTTAAGGGTTTTGTTTTTCAATTTGATTCATTTTATCTTGATTTTGTTGTTTTCATTTGTTAATTATTGTTAACATGTGATGAACAATTAATAAAATTTTATATTTTTTGTAGGAATCTTGTCCAATTGGTGGAAAAATTTGGGCTCAATAACTGGGTAGAGATTGCTAAGTTTATGAAAGGAAGAATCGGAAAACAGTGCAGGGAGAGATGGTACAACCATCTTCAACCGGACATTAAGGTGCCTTTTTAAAAAAAAAAAAAAAAAAAATCCTTTTTAGCTTTTATCCCTTACACATACACTTAGAATGCTCTTATCTTCTATGTTTTTACATGTACATATAGATGCATCAAATGGATAAAAAAAAAATAAGAATGCATGTCCATTCAAAGCAATTAATTATCACTTGTACATGTTTTATAACGACGAACGAAATTCTCATAACTAATGTTTATTTTGATACATGTCTTAAAGTTCTTAAGTAGTAATAGTTTAATTATCACATTATATTGGATATAAATGAACTTTTTGTGATATTTGAACATCAGTGCAGTTGTCTATTTTTCTGAATAAATATTGGACTAAATTTATAAACAATGTTCTAGGTATTTGTCTTGAAAACTAACATTGTTCTAAATATTGTTGTTATTGTCTATCTCACAAAGAGATAGTTTCTTTTGTTTTTTCCACCATTTTAAAAATGCAAACGACAGATATATTATATTTTAGTTAAAAGCAAAGAAACAATGTCCATATTTTACTTTTCTTAAAATAAAAAAAAAAAATGTCCATATGTATGAATGCTTTATTCATAATTATTTTCAATGAATTTCTTTTGTTTGAAACATAATATTGAGATAAGTTTGGTTCATAAAAATGCATCCTTACCAATTTCTTATTTTAGTTAATTTTGAACAAATAGATACTTCAGGAATTGTATCTCGTTTTTACACTTTTCCTTTCATGTAGGTTGTTTTAATTTCTCGTTAGACTAATAATTAACTTCAATTTATAGATGCTGTTTAAAATCTTCAATTGTTGCTTCATACCAAACTAATTCTTACCAAACAAACTATGCTCTTTTATTAATTACGTATAAAAATGTTATCATTAATTCTCATACATGTAAATTGTTGATGTTGAATGCTATGATAGATACGAGAGTTGTATACATCAATTGTGATCTGGGACACAATCAAATCTTAAATTCAATTACCATTTTTGAAATGGGGATCTTCATCAATTTTATTATAAATCATATATCTAATCCACACATATATTGTTTGGTGTGATTGTAGAAGGATACATGGAGCGAAGAGGAAGACATATTATTGATCAAAGCTCACAAGATGATTGGGAACAAGTGGGCTGAAATTGCAAGTAGATTGCCTGGGCGCACTGAGAACTCCATCAAGAACCGCTGGAACTCCACTAAGCGCAGTCTGAATGCTACAAGTAGGCCAAACCGCCGAAATAGTTTGAGAGGAACATTACTTCATAATTACATTAGCGAAATCACCTATGCGGAGAATGTTCAAAAGGAGCTGACTAATTCCATGAACATAGTGCAGATTGCGAATCCGACAAATTTCAACATTACCAATACCAACCCTGGTCCTAGTCATGTTGGTTATGAAGTTGCAAACAATGGTTTTAGCTCTGAAGGTTTCGCTGCTCCTGTGGAAGAAGATGGTGGCTATGCAGCAATGATGCTCAACGGTGATGAAGGAATGACCAGTGGGTATGGCACAATGAATTATGGGTTCGGATCGTATGGAATGGAGTTTTTTCCTGAGTTTTTACCTCATGGAGATAATCTATAG |
| Protein sequence |
>MS.gene037211.t1 MELDPSFSEKISYLLGDFPKIPQEKSEIPATVLLQPSSTISPTPSHYTSFLYNQSSQFNEHPLNMANQDFRVSTVVPSYINEFSMIPTPSYRGANTNRSQSDFFNDQIPITFTPNNNKNEFMHEHLNTGKEIWDFSQQNLFQHGQTSQSHFSPSLSTSVVYERLNTNKGIWDFSQQNLFQYGETSQSQVSPSLPTSVVYDENPSLLVPRLQANISCIGGFENNTQHNDEGLIPNNQKRQKRVDKTIDIQQRDFNTSKRLWTSDEDRNLVQLVEKFGLNNWVEIAKFMKGRIGKQCRERWYNHLQPDIKKDTWSEEEDILLIKAHKMIGNKWAEIASRLPGRTENSIKNRWNSTKRSLNATSRPNRRNSLRGTLLHNYISEITYAENVQKELTNSMNIVQIANPTNFNITNTNPGPSHVGYEVANNGFSSEGFAAPVEEDGGYAAMMLNGDEGMTSGYGTMNYGFGSYGMEFFPEFLPHGDNL |
