Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037220.t1 | RHN45260.1 | 74.2 | 155 | 30 | 2 | 2 | 156 | 288 | 432 | 2.40E-53 | 218.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037220.t1 | Q9S7L2 | 61.8 | 55 | 21 | 0 | 3 | 57 | 287 | 341 | 8.6e-12 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037220.t1 | G7KR91 | 74.2 | 155 | 30 | 2 | 2 | 156 | 214 | 358 | 1.7e-53 | 218.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene037220.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037220.t1 | MTR_7g037130 | 74.194 | 155 | 30 | 2 | 2 | 156 | 214 | 358 | 2.37e-73 | 224 |
| MS.gene037220.t1 | MTR_7g037260 | 74.194 | 155 | 30 | 2 | 2 | 156 | 214 | 358 | 1.90e-72 | 221 |
| MS.gene037220.t1 | MTR_7g451170 | 68.387 | 155 | 47 | 1 | 2 | 156 | 260 | 412 | 4.34e-69 | 214 |
| MS.gene037220.t1 | MTR_4g088015 | 68.387 | 155 | 47 | 1 | 2 | 156 | 290 | 442 | 1.84e-63 | 201 |
| MS.gene037220.t1 | MTR_7g035350 | 53.642 | 151 | 53 | 4 | 3 | 138 | 295 | 443 | 3.52e-43 | 148 |
| MS.gene037220.t1 | MTR_1g057980 | 50.658 | 152 | 54 | 5 | 2 | 138 | 149 | 294 | 4.52e-35 | 124 |
| MS.gene037220.t1 | MTR_7g450950 | 48.438 | 128 | 40 | 3 | 3 | 128 | 227 | 330 | 7.29e-28 | 105 |
| MS.gene037220.t1 | MTR_3g077650 | 74.510 | 51 | 13 | 0 | 3 | 53 | 251 | 301 | 4.17e-19 | 83.2 |
| MS.gene037220.t1 | MTR_7g035300 | 35.714 | 140 | 38 | 3 | 2 | 141 | 133 | 220 | 6.73e-19 | 80.1 |
| MS.gene037220.t1 | MTR_4g105660 | 66.667 | 51 | 17 | 0 | 3 | 53 | 250 | 300 | 2.44e-17 | 78.2 |
| MS.gene037220.t1 | MTR_5g070020 | 54.545 | 55 | 23 | 1 | 3 | 55 | 192 | 246 | 3.68e-14 | 68.9 |
| MS.gene037220.t1 | MTR_3g463400 | 48.438 | 64 | 28 | 2 | 2 | 60 | 202 | 265 | 1.04e-13 | 67.8 |
| MS.gene037220.t1 | MTR_5g049190 | 55.556 | 63 | 25 | 1 | 3 | 65 | 89 | 148 | 6.25e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037220.t1 | AT4G18770 | 66.667 | 51 | 17 | 0 | 3 | 53 | 287 | 337 | 1.27e-16 | 75.9 |
| MS.gene037220.t1 | AT5G40360 | 56.897 | 58 | 23 | 1 | 2 | 57 | 227 | 284 | 6.27e-14 | 68.2 |
| MS.gene037220.t1 | AT3G27785 | 56.667 | 60 | 22 | 2 | 3 | 58 | 259 | 318 | 7.76e-14 | 68.2 |
| MS.gene037220.t1 | AT5G58850 | 67.442 | 43 | 14 | 0 | 1 | 43 | 173 | 215 | 4.60e-13 | 65.9 |
| MS.gene037220.t1 | AT5G11050 | 68.421 | 38 | 12 | 0 | 3 | 40 | 175 | 212 | 2.29e-11 | 60.8 |
| MS.gene037220.t1 | AT1G57560 | 32.558 | 129 | 69 | 5 | 2 | 121 | 84 | 203 | 8.90e-11 | 58.9 |
Find 40 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTAGAACTTTCATAATT+TGG | 0.113072 | 7.3:+62637871 | None:intergenic |
| GTTACCCCGATCCTTCTTCT+TGG | 0.289472 | 7.3:+62638005 | None:intergenic |
| ACTCTAGAAGAAGAAATTAA+TGG | 0.296043 | 7.3:-62637823 | MS.gene037220:CDS |
| TTTATGCTTTATAGGAATCT+TGG | 0.324162 | 7.3:+62637702 | None:intergenic |
| TTATGCTTTATAGGAATCTT+GGG | 0.335332 | 7.3:+62637703 | None:intergenic |
| TCAGGTATCACAATGAACTA+TGG | 0.354032 | 7.3:-62637754 | MS.gene037220:CDS |
| AACTATGGGTTTGGATCATA+TGG | 0.356714 | 7.3:-62637739 | MS.gene037220:CDS |
| TATGTGAGCATGATGAATAT+TGG | 0.411232 | 7.3:-62637901 | MS.gene037220:CDS |
| ATCACAATGAACTATGGGTT+TGG | 0.419328 | 7.3:-62637748 | MS.gene037220:CDS |
| TGAAATTGCAAAGAGATTGC+CGG | 0.432391 | 7.3:-62638085 | MS.gene037220:CDS |
| GATGGAATGTCAAGTGGATC+AGG | 0.444794 | 7.3:-62637772 | MS.gene037220:CDS |
| GAAATTGCAAAGAGATTGCC+GGG | 0.462619 | 7.3:-62638084 | MS.gene037220:CDS |
| TCATCTCCATTGAACATCAT+TGG | 0.465410 | 7.3:+62637793 | None:intergenic |
| GCATGATGAATATTGGAAAT+CGG | 0.473174 | 7.3:-62637894 | MS.gene037220:CDS |
| GAAAACCAAGAAGAAGGATC+GGG | 0.491124 | 7.3:-62638010 | MS.gene037220:CDS |
| TTATGCTAAGGAAGTTGAAA+AGG | 0.493259 | 7.3:-62637938 | MS.gene037220:CDS |
| GATCGAAGCTCACATGATAG+TGG | 0.495487 | 7.3:-62638121 | None:intergenic |
| ACATGATAGTGGGGAATAAG+TGG | 0.500958 | 7.3:-62638110 | MS.gene037220:CDS |
| ATCGAAGCTCACATGATAGT+GGG | 0.522647 | 7.3:-62638120 | None:intergenic |
| ATGATGTTCAATGGAGATGA+TGG | 0.528624 | 7.3:-62637790 | MS.gene037220:CDS |
| AAGATATATAGAGACCCTTA+AGG | 0.531255 | 7.3:-62637658 | None:intergenic |
| CAGGTATCACAATGAACTAT+GGG | 0.534571 | 7.3:-62637753 | MS.gene037220:CDS |
| TCGTCTGAAAACCAAGAAGA+AGG | 0.548747 | 7.3:-62638016 | MS.gene037220:CDS |
| AGCATTCCAATGGTTCTTGA+CGG | 0.549868 | 7.3:+62638047 | None:intergenic |
| TGAAAACCAAGAAGAAGGAT+CGG | 0.557226 | 7.3:-62638011 | MS.gene037220:CDS |
| AGAACTCCGTCAAGAACCAT+TGG | 0.558781 | 7.3:-62638053 | MS.gene037220:CDS |
| AATGGAAGTCAGTTATGCTA+AGG | 0.573152 | 7.3:-62637950 | MS.gene037220:CDS |
| GGATCGGGGTAACAGTTCAA+AGG | 0.574761 | 7.3:-62637995 | MS.gene037220:CDS |
| TGGGTTTGGATCATATGGAA+TGG | 0.579274 | 7.3:-62637734 | MS.gene037220:CDS |
| ACGGAGTTCTCAGTGCGCCC+CGG | 0.590036 | 7.3:+62638066 | None:intergenic |
| GATCGGGGTAACAGTTCAAA+GGG | 0.597225 | 7.3:-62637994 | MS.gene037220:CDS |
| TATGTGCCAATGATGTTCAA+TGG | 0.598075 | 7.3:-62637799 | MS.gene037220:CDS |
| CATGATAGTGGGGAATAAGT+GGG | 0.602868 | 7.3:-62638109 | MS.gene037220:CDS |
| GATGCTTAGTAGCATTCCAA+TGG | 0.605219 | 7.3:+62638037 | None:intergenic |
| TATGCTTTATAGGAATCTTG+GGG | 0.614042 | 7.3:+62637704 | None:intergenic |
| GGAGATGATGGAATGTCAAG+TGG | 0.649882 | 7.3:-62637778 | MS.gene037220:CDS |
| CTCTATATATCTTCTCCGCG+AGG | 0.653577 | 7.3:+62637668 | None:intergenic |
| TCGAAGCTCACATGATAGTG+GGG | 0.690915 | 7.3:-62638119 | None:intergenic |
| AAAACCAAGAAGAAGGATCG+GGG | 0.703350 | 7.3:-62638009 | MS.gene037220:CDS |
| AAATTGCAAAGAGATTGCCG+GGG | 0.789975 | 7.3:-62638083 | MS.gene037220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTGATTTATGCTTTAT+AGG | + | chr7.3:62638077-62638096 | None:intergenic | 15.0% |
| ! | CTCTTAGAACTTTCATAATT+TGG | + | chr7.3:62637900-62637919 | None:intergenic | 25.0% |
| ! | TAAATCAAAAATTAACCTCG+CGG | - | chr7.3:62638085-62638104 | MS.gene037220:CDS | 25.0% |
| ! | TTATGCTTTATAGGAATCTT+GGG | + | chr7.3:62638068-62638087 | None:intergenic | 25.0% |
| ! | TTTATGCTTTATAGGAATCT+TGG | + | chr7.3:62638069-62638088 | None:intergenic | 25.0% |
| !! | ACTCTAGAAGAAGAAATTAA+TGG | - | chr7.3:62637945-62637964 | MS.gene037220:CDS | 25.0% |
| !!! | ACTACTTTTGAAGTACATAA+TGG | - | chr7.3:62637800-62637819 | MS.gene037220:CDS | 25.0% |
| GCATGATGAATATTGGAAAT+CGG | - | chr7.3:62637874-62637893 | MS.gene037220:CDS | 30.0% | |
| TATGCTTTATAGGAATCTTG+GGG | + | chr7.3:62638067-62638086 | None:intergenic | 30.0% | |
| TATGTGAGCATGATGAATAT+TGG | - | chr7.3:62637867-62637886 | MS.gene037220:CDS | 30.0% | |
| TTATGCTAAGGAAGTTGAAA+AGG | - | chr7.3:62637830-62637849 | MS.gene037220:CDS | 30.0% | |
| !!! | AAGAGTGATTTTAGCTTTGA+AGG | - | chr7.3:62637915-62637934 | MS.gene037220:CDS | 30.0% |
| AACTATGGGTTTGGATCATA+TGG | - | chr7.3:62638029-62638048 | MS.gene037220:CDS | 35.0% | |
| AATGGAAGTCAGTTATGCTA+AGG | - | chr7.3:62637818-62637837 | MS.gene037220:CDS | 35.0% | |
| ATCACAATGAACTATGGGTT+TGG | - | chr7.3:62638020-62638039 | MS.gene037220:CDS | 35.0% | |
| ATGATGTTCAATGGAGATGA+TGG | - | chr7.3:62637978-62637997 | MS.gene037220:CDS | 35.0% | |
| CAGGTATCACAATGAACTAT+GGG | - | chr7.3:62638015-62638034 | MS.gene037220:CDS | 35.0% | |
| TATGTGCCAATGATGTTCAA+TGG | - | chr7.3:62637969-62637988 | MS.gene037220:CDS | 35.0% | |
| TCAGGTATCACAATGAACTA+TGG | - | chr7.3:62638014-62638033 | MS.gene037220:CDS | 35.0% | |
| TCATCTCCATTGAACATCAT+TGG | + | chr7.3:62637978-62637997 | None:intergenic | 35.0% | |
| TGAAAACCAAGAAGAAGGAT+CGG | - | chr7.3:62637757-62637776 | MS.gene037220:CDS | 35.0% | |
| TGAAATTGCAAAGAGATTGC+CGG | - | chr7.3:62637683-62637702 | MS.gene037220:CDS | 35.0% | |
| AAAACCAAGAAGAAGGATCG+GGG | - | chr7.3:62637759-62637778 | MS.gene037220:CDS | 40.0% | |
| AAATTGCAAAGAGATTGCCG+GGG | - | chr7.3:62637685-62637704 | MS.gene037220:CDS | 40.0% | |
| AGCATTCCAATGGTTCTTGA+CGG | + | chr7.3:62637724-62637743 | None:intergenic | 40.0% | |
| GAAAACCAAGAAGAAGGATC+GGG | - | chr7.3:62637758-62637777 | MS.gene037220:CDS | 40.0% | |
| GAAATTGCAAAGAGATTGCC+GGG | - | chr7.3:62637684-62637703 | MS.gene037220:CDS | 40.0% | |
| GATGCTTAGTAGCATTCCAA+TGG | + | chr7.3:62637734-62637753 | None:intergenic | 40.0% | |
| TCGTCTGAAAACCAAGAAGA+AGG | - | chr7.3:62637752-62637771 | MS.gene037220:CDS | 40.0% | |
| TGGGTTTGGATCATATGGAA+TGG | - | chr7.3:62638034-62638053 | MS.gene037220:CDS | 40.0% | |
| AGAACTCCGTCAAGAACCAT+TGG | - | chr7.3:62637715-62637734 | MS.gene037220:CDS | 45.0% | |
| CTCTATATATCTTCTCCGCG+AGG | + | chr7.3:62638103-62638122 | None:intergenic | 45.0% | |
| GATCGGGGTAACAGTTCAAA+GGG | - | chr7.3:62637774-62637793 | MS.gene037220:CDS | 45.0% | |
| GATGGAATGTCAAGTGGATC+AGG | - | chr7.3:62637996-62638015 | MS.gene037220:CDS | 45.0% | |
| !! | GGAGATGATGGAATGTCAAG+TGG | - | chr7.3:62637990-62638009 | MS.gene037220:CDS | 45.0% |
| GGATCGGGGTAACAGTTCAA+AGG | - | chr7.3:62637773-62637792 | MS.gene037220:CDS | 50.0% | |
| GTTACCCCGATCCTTCTTCT+TGG | + | chr7.3:62637766-62637785 | None:intergenic | 50.0% | |
| ACGGAGTTCTCAGTGCGCCC+CGG | + | chr7.3:62637705-62637724 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 62637660 | 62638130 | 62637660 | ID=MS.gene037220 |
| chr7.3 | mRNA | 62637660 | 62638130 | 62637660 | ID=MS.gene037220.t1;Parent=MS.gene037220 |
| chr7.3 | exon | 62637660 | 62638130 | 62637660 | ID=MS.gene037220.t1.exon1;Parent=MS.gene037220.t1 |
| chr7.3 | CDS | 62637660 | 62638130 | 62637660 | ID=cds.MS.gene037220.t1;Parent=MS.gene037220.t1 |
| Gene Sequence |
| Protein sequence |
