AlfalfaGEDB | MS.gene037221

M. sativa cultivar XinJiangDaYe / MS.gene037221

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037221.t1	KEH15540.1	77.3	132	28	1	1	132	1	130	9.50E-54	219.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037221.t1	Q9SZL8	29.2	130	81	4	15	133	47	176	2.3e-08	60.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037221.t1	A0A072TPN7	77.3	132	28	1	1	132	1	130	6.8e-54	219.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene037221.t1	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037221.t1	MTR_0856s0010	76.692	133	29	1	1	133	1	131	1.66e-74	219
MS.gene037221.t1	MTR_3g006370	37.383	107	63	2	30	132	44	150	1.01e-12	63.2
MS.gene037221.t1	MTR_7g061980	32.673	101	64	2	35	132	9	108	4.34e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene037221.t1	AT2G43280	33.645	107	67	2	30	132	16	122	1.21e-12	62.8
MS.gene037221.t1	AT3G07500	35.644	101	62	2	35	132	32	132	6.25e-12	60.8

Find 20 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTATCAAATTGAAAGAAGA+TGG	0.385199	7.3:-62631307	MS.gene037221:CDS
TTGAAGACACGTCCGACAAT+GGG	0.482433	7.3:-62631352	MS.gene037221:CDS
AATTTGATACAAATCTTAGC+AGG	0.483019	7.3:+62631319	None:intergenic
ATTCTTGCATGCACAAGAGA+AGG	0.499742	7.3:-62631403	MS.gene037221:CDS
AAGATTAGAACATCGAGAAA+AGG	0.502639	7.3:-62631451	MS.gene037221:CDS
GTATCTTGATGAGAATGATC+AGG	0.505938	7.3:+62631259	None:intergenic
TCAAATGTTGTTGTGGATTG+TGG	0.509257	7.3:-62631631	MS.gene037221:CDS
CATCGAGAAAAGGACGAGAT+GGG	0.520186	7.3:-62631441	MS.gene037221:CDS
AAGAAGATGATGATGCATGC+TGG	0.530084	7.3:-62631561	MS.gene037221:CDS
GATGGTGAAGGTATAGAAGT+TGG	0.532672	7.3:-62631601	MS.gene037221:CDS
ACATCGAGAAAAGGACGAGA+TGG	0.535829	7.3:-62631442	MS.gene037221:CDS
GGATAGTTCAAATGTTGTTG+TGG	0.550233	7.3:-62631638	MS.gene037221:CDS
GTGGATTGTGGTAGTACTGA+TGG	0.559597	7.3:-62631619	MS.gene037221:CDS
TTCTTGCATGCACAAGAGAA+GGG	0.575717	7.3:-62631402	MS.gene037221:CDS
ATTGAAGACACGTCCGACAA+TGG	0.579883	7.3:-62631353	MS.gene037221:CDS
AGAAGATGATGATGCATGCT+GGG	0.582547	7.3:-62631560	MS.gene037221:CDS
ATCGAGAAAAGGACGAGATG+GGG	0.592032	7.3:-62631440	MS.gene037221:CDS
AATTGAAAGAAGATGGATTG+TGG	0.618386	7.3:-62631300	MS.gene037221:CDS
GAGAAAAGGACGAGATGGGG+AGG	0.628028	7.3:-62631437	MS.gene037221:CDS
TGTGGTAGTACTGATGGTGA+AGG	0.646539	7.3:-62631613	MS.gene037221:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GAAAAACACATATCTATTTT+CGG	+	chr7.3:62631348-62631367	None:intergenic	20.0%
!	AATTTGATACAAATCTTAGC+AGG	+	chr7.3:62631564-62631583	None:intergenic	25.0%
!	TGTATCAAATTGAAAGAAGA+TGG	-	chr7.3:62631573-62631592	MS.gene037221:CDS	25.0%
!!!	AAAGAGTATGCTTTTAGAAA+GGG	-	chr7.3:62631396-62631415	MS.gene037221:CDS	25.0%
!!!	TAAAGAGTATGCTTTTAGAA+AGG	-	chr7.3:62631395-62631414	MS.gene037221:CDS	25.0%
	AAGATTAGAACATCGAGAAA+AGG	-	chr7.3:62631429-62631448	MS.gene037221:CDS	30.0%
!!	AATTGAAAGAAGATGGATTG+TGG	-	chr7.3:62631580-62631599	MS.gene037221:CDS	30.0%
!!!	CTTTTAGAAAGGGTTTTGAA+TGG	-	chr7.3:62631406-62631425	MS.gene037221:CDS	30.0%
	GGATAGTTCAAATGTTGTTG+TGG	-	chr7.3:62631242-62631261	MS.gene037221:CDS	35.0%
	GTATCTTGATGAGAATGATC+AGG	+	chr7.3:62631624-62631643	None:intergenic	35.0%
!	TCAAATGTTGTTGTGGATTG+TGG	-	chr7.3:62631249-62631268	MS.gene037221:CDS	35.0%
!!!	GGACAATTTTTTCCCATTGT+CGG	+	chr7.3:62631543-62631562	None:intergenic	35.0%
	ATTCTTGCATGCACAAGAGA+AGG	-	chr7.3:62631477-62631496	MS.gene037221:CDS	40.0%
	TTCTTGCATGCACAAGAGAA+GGG	-	chr7.3:62631478-62631497	MS.gene037221:CDS	40.0%
!	AAGAAGATGATGATGCATGC+TGG	-	chr7.3:62631319-62631338	MS.gene037221:CDS	40.0%
!	AGAAGATGATGATGCATGCT+GGG	-	chr7.3:62631320-62631339	MS.gene037221:CDS	40.0%
!	GATGGTGAAGGTATAGAAGT+TGG	-	chr7.3:62631279-62631298	MS.gene037221:CDS	40.0%
	ACATCGAGAAAAGGACGAGA+TGG	-	chr7.3:62631438-62631457	MS.gene037221:CDS	45.0%
	ATCGAGAAAAGGACGAGATG+GGG	-	chr7.3:62631440-62631459	MS.gene037221:CDS	45.0%
	ATTGAAGACACGTCCGACAA+TGG	-	chr7.3:62631527-62631546	MS.gene037221:CDS	45.0%
	CATCGAGAAAAGGACGAGAT+GGG	-	chr7.3:62631439-62631458	MS.gene037221:CDS	45.0%
	TTGAAGACACGTCCGACAAT+GGG	-	chr7.3:62631528-62631547	MS.gene037221:CDS	45.0%
!	GTGGATTGTGGTAGTACTGA+TGG	-	chr7.3:62631261-62631280	MS.gene037221:CDS	45.0%
!!	TGTGGTAGTACTGATGGTGA+AGG	-	chr7.3:62631267-62631286	MS.gene037221:CDS	45.0%
	GAGAAAAGGACGAGATGGGG+AGG	-	chr7.3:62631443-62631462	MS.gene037221:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	62631240	62631662	62631240	ID=MS.gene037221
chr7.3	mRNA	62631240	62631662	62631240	ID=MS.gene037221.t1;Parent=MS.gene037221
chr7.3	exon	62631240	62631662	62631240	ID=MS.gene037221.t1.exon1;Parent=MS.gene037221.t1
chr7.3	CDS	62631240	62631662	62631240	ID=cds.MS.gene037221.t1;Parent=MS.gene037221.t1

Gene Sequence

>MS.gene037221

ATGGATAGTTCAAATGTTGTTGTGGATTGTGGTAGTACTGATGGTGAAGGTATAGAAGTTGGCAGCTTTAATGTTGAAGAAGAAGATGATGATGCATGCTGGGAGCCGAAAATAGATATGTGTTTTTCTAGCTTAGATGAAGTTAAATCATTTTATAAAGAGTATGCTTTTAGAAAGGGTTTTGAATGGAAGATTAGAACATCGAGAAAAGGACGAGATGGGGAGGTCTGCTACTTAATTCTTGCATGCACAAGAGAAGGGTCTCAAGTGACAAAAAGTCTGTGTACATTGAAGACACGTCCGACAATGGGAAAAAATTGTCCTGCTAAGATTTGTATCAAATTGAAAGAAGATGGATTGTGGTACATTCAAAAATTTGAACCTGATCATTCTCATCAAGATACAATGCTAGTACTTATGTAA

Protein sequence

>MS.gene037221.t1

MDSSNVVVDCGSTDGEGIEVGSFNVEEEDDDACWEPKIDMCFSSLDEVKSFYKEYAFRKGFEWKIRTSRKGRDGEVCYLILACTREGSQVTKSLCTLKTRPTMGKNCPAKICIKLKEDGLWYIQKFEPDHSHQDTMLVLM