AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037224


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037224.t1 MTR_7g037260 92.258 155 12 0 5 159 197 351 1.53e-100 293
MS.gene037224.t1 MTR_7g037130 88.750 160 15 1 3 159 192 351 2.45e-99 290
MS.gene037224.t1 MTR_7g451170 71.166 163 39 1 5 159 243 405 1.32e-76 234
MS.gene037224.t1 MTR_4g088015 69.325 163 42 1 5 159 273 435 9.67e-70 217
MS.gene037224.t1 MTR_7g035350 52.976 168 56 4 5 150 277 443 4.90e-47 158
MS.gene037224.t1 MTR_1g057980 53.049 164 58 4 5 150 132 294 6.36e-42 141
MS.gene037224.t1 MTR_7g450950 52.174 138 48 3 5 140 209 330 4.28e-35 125
MS.gene037224.t1 MTR_7g035300 39.610 154 49 3 6 159 117 226 1.25e-27 103
MS.gene037224.t1 MTR_3g077650 69.565 69 21 0 5 73 233 301 5.88e-27 105
MS.gene037224.t1 MTR_4g105660 65.217 69 24 0 5 73 232 300 9.08e-26 101
MS.gene037224.t1 MTR_5g070020 56.164 73 30 1 5 75 174 246 2.33e-22 91.7
MS.gene037224.t1 MTR_3g463400 53.165 79 32 2 5 78 185 263 3.31e-22 92.0
MS.gene037224.t1 MTR_5g049190 36.313 179 76 8 5 159 71 235 8.82e-20 82.8
MS.gene037224.t1 MTR_4g019370 56.863 51 22 0 5 55 90 140 1.04e-15 73.2
MS.gene037224.t1 MTR_1g026870 55.102 49 22 0 5 53 137 185 1.59e-14 70.5
MS.gene037224.t1 MTR_3g110028 53.061 49 23 0 5 53 137 185 2.01e-14 70.1
MS.gene037224.t1 MTR_4g094982 55.769 52 23 0 2 53 54 105 4.55e-14 68.2
MS.gene037224.t1 MTR_5g016510 55.769 52 23 0 2 53 54 105 6.59e-14 67.8
MS.gene037224.t1 MTR_7g061330 55.102 49 22 0 5 53 165 213 1.82e-13 67.0
MS.gene037224.t1 MTR_7g461410 55.102 49 22 0 5 53 165 213 1.82e-13 67.0
MS.gene037224.t1 MTR_7g061330 55.102 49 22 0 5 53 129 177 2.72e-13 66.6
MS.gene037224.t1 MTR_5g041570 50.909 55 27 0 5 59 186 240 6.30e-13 65.5
MS.gene037224.t1 MTR_5g082910 61.905 42 16 0 8 49 74 115 1.50e-12 63.9
MS.gene037224.t1 MTR_2g064160 47.059 51 27 0 7 57 68 118 7.09e-12 62.4
MS.gene037224.t1 MTR_6g055910 29.114 158 80 7 10 142 5 155 7.97e-12 61.2
MS.gene037224.t1 MTR_4g082040 47.170 53 28 0 5 57 157 209 1.33e-11 61.6
MS.gene037224.t1 MTR_4g073420 51.852 54 23 1 8 61 70 120 1.42e-11 61.2
MS.gene037224.t1 MTR_6g055910 29.114 158 80 7 10 142 46 196 1.70e-11 60.8
MS.gene037224.t1 MTR_5g010650 58.140 43 18 0 5 47 162 204 1.84e-11 61.2
MS.gene037224.t1 MTR_3g101290 55.556 45 20 0 9 53 93 137 2.37e-11 60.1
MS.gene037224.t1 MTR_6g055910 29.114 158 80 7 10 142 75 225 2.45e-11 60.5
MS.gene037224.t1 MTR_8g095390 46.939 49 26 0 5 53 67 115 3.20e-11 60.5
MS.gene037224.t1 MTR_5g029840 32.967 91 50 1 8 98 70 149 3.75e-11 60.1
MS.gene037224.t1 MTR_6g015455 55.319 47 21 0 7 53 59 105 3.83e-11 60.5
MS.gene037224.t1 MTR_6g074860 49.057 53 27 0 5 57 56 108 5.03e-11 59.3
MS.gene037224.t1 MTR_8g017390 41.509 53 31 0 7 59 70 122 5.65e-11 60.1
MS.gene037224.t1 MTR_8g017500 46.809 47 25 0 7 53 69 115 6.43e-11 59.7
MS.gene037224.t1 MTR_3g052450 50.980 51 24 1 5 54 95 145 7.59e-11 59.7
MS.gene037224.t1 MTR_8g017440 46.809 47 25 0 7 53 69 115 8.55e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037224.t1 AT4G18770 65.217 69 24 0 5 73 269 337 1.25e-25 101
MS.gene037224.t1 AT5G40360 58.667 75 29 1 5 77 210 284 1.12e-21 89.7
MS.gene037224.t1 AT3G27785 59.740 77 27 2 5 77 241 317 2.85e-21 89.4
MS.gene037224.t1 AT5G11050 64.286 56 20 0 5 60 157 212 1.76e-20 87.0
MS.gene037224.t1 AT5G58850 38.462 143 67 5 5 129 157 296 6.20e-20 85.5
MS.gene037224.t1 AT2G25230 50.685 73 36 0 5 77 78 150 3.19e-19 81.3
MS.gene037224.t1 AT5G40430 47.945 73 38 0 5 77 105 177 7.23e-17 75.5
MS.gene037224.t1 AT3G55730 51.667 60 28 1 3 61 105 164 4.81e-15 71.6
MS.gene037224.t1 AT3G09230 46.269 67 36 0 4 70 106 172 1.57e-14 70.1
MS.gene037224.t1 AT5G17800 33.582 134 81 2 5 138 145 270 5.55e-14 68.2
MS.gene037224.t1 AT4G32730 53.061 49 23 0 5 53 139 187 1.80e-13 67.4
MS.gene037224.t1 AT4G32730 53.061 49 23 0 5 53 139 187 1.80e-13 67.4
MS.gene037224.t1 AT4G32730 53.061 49 23 0 5 53 139 187 1.80e-13 67.4
MS.gene037224.t1 AT4G32730 53.061 49 23 0 5 53 139 187 1.80e-13 67.4
MS.gene037224.t1 AT4G32730 53.061 49 23 0 5 53 139 187 2.35e-13 67.0
MS.gene037224.t1 AT5G11510 48.980 49 25 0 5 53 133 181 3.94e-13 66.2
MS.gene037224.t1 AT5G11510 48.980 49 25 0 5 53 133 181 3.94e-13 66.2
MS.gene037224.t1 AT5G11510 48.980 49 25 0 5 53 133 181 3.94e-13 66.2
MS.gene037224.t1 AT5G11510 48.980 49 25 0 5 53 133 181 4.04e-13 66.2
MS.gene037224.t1 AT5G11510 48.980 49 25 0 5 53 133 181 4.10e-13 66.2
MS.gene037224.t1 AT1G69560 42.857 70 40 0 5 74 175 244 4.19e-13 65.9
MS.gene037224.t1 AT1G69560 42.857 70 40 0 5 74 159 228 5.73e-13 65.5
MS.gene037224.t1 AT5G02320 51.020 49 24 0 5 53 179 227 5.85e-13 65.9
MS.gene037224.t1 AT5G02320 51.020 49 24 0 5 53 179 227 5.85e-13 65.9
MS.gene037224.t1 AT5G02320 51.020 49 24 0 5 53 119 167 7.59e-13 65.5
MS.gene037224.t1 AT3G09370 53.061 49 23 0 5 53 195 243 9.25e-13 65.1
MS.gene037224.t1 AT3G09370 53.061 49 23 0 5 53 187 235 9.40e-13 65.1
MS.gene037224.t1 AT3G09370 53.061 49 23 0 5 53 182 230 1.09e-12 65.1
MS.gene037224.t1 AT4G00540 54.000 50 23 0 4 53 157 206 1.33e-12 64.7
MS.gene037224.t1 AT4G00540 54.000 50 23 0 4 53 155 204 1.56e-12 64.3
MS.gene037224.t1 AT1G09540 30.827 133 80 4 7 136 69 192 1.56e-12 64.3
MS.gene037224.t1 AT4G37260 51.852 54 25 1 7 59 67 120 2.13e-12 63.9
MS.gene037224.t1 AT5G67300 48.333 60 31 0 2 61 55 114 2.72e-12 63.5
MS.gene037224.t1 AT1G57560 27.950 161 95 4 7 159 69 216 2.99e-12 63.2
MS.gene037224.t1 AT3G12720 49.020 51 26 0 7 57 79 129 3.10e-12 63.2
MS.gene037224.t1 AT2G23290 58.696 46 19 0 8 53 68 113 3.19e-12 63.2
MS.gene037224.t1 AT4G09460 30.000 140 88 2 9 141 71 207 3.77e-12 62.4
MS.gene037224.t1 AT4G38620 51.852 54 23 1 8 61 70 120 4.95e-12 62.4
MS.gene037224.t1 AT1G26780 51.923 52 25 0 5 56 150 201 5.18e-12 62.8
MS.gene037224.t1 AT1G26780 51.923 52 25 0 5 56 150 201 7.00e-12 62.0
MS.gene037224.t1 AT5G14750 30.252 119 74 3 7 121 12 125 9.97e-12 59.7
MS.gene037224.t1 AT5G14750 30.252 119 74 3 7 121 12 125 9.97e-12 59.7
MS.gene037224.t1 AT2G32460 51.923 52 24 1 5 55 73 124 1.57e-11 61.6
MS.gene037224.t1 AT2G32460 51.923 52 24 1 5 55 61 112 1.62e-11 61.6
MS.gene037224.t1 AT5G14750 30.252 119 74 3 7 121 39 152 2.28e-11 59.3
MS.gene037224.t1 AT1G22640 44.828 58 32 0 8 65 13 70 2.62e-11 59.7
MS.gene037224.t1 AT3G53200 34.074 135 63 6 9 120 35 166 2.76e-11 59.7
MS.gene037224.t1 AT3G50060 54.348 46 21 0 8 53 61 106 2.90e-11 60.5
MS.gene037224.t1 AT4G33450 51.923 52 25 0 5 56 71 122 3.12e-11 60.1
MS.gene037224.t1 AT1G17950 41.791 67 36 1 5 68 57 123 3.97e-11 59.7
MS.gene037224.t1 AT3G53200 34.074 135 63 6 9 120 68 199 4.97e-11 59.3
MS.gene037224.t1 AT3G28470 48.837 43 22 0 6 48 68 110 5.84e-11 59.7
MS.gene037224.t1 AT2G39880 41.509 53 31 0 5 57 102 154 6.71e-11 59.7
MS.gene037224.t1 AT5G14750 30.769 117 72 3 9 121 75 186 7.12e-11 58.5
MS.gene037224.t1 AT3G11440 46.154 52 27 1 5 55 96 147 8.50e-11 59.7
MS.gene037224.t1 AT4G28110 51.111 45 22 0 11 55 73 117 8.51e-11 58.9

Find 40 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTCTTCTCGACTTTC+TGG 0.103042 7.3:+62579506 None:intergenic
CTCTCAGAACTTTCATAATT+TGG 0.107846 7.3:+62579364 None:intergenic
TCCTGCTTTATAGGAATTTC+AGG 0.178416 7.3:+62579220 None:intergenic
AGGTCAATTTCCTGCTTTAT+AGG 0.200831 7.3:+62579211 None:intergenic
TAGCCACCAATTTCATCTTC+TGG 0.310919 7.3:+62579313 None:intergenic
ACTACTCCAGAAGATGAAAT+TGG 0.319338 7.3:-62579319 MS.gene037224:CDS
TATCTGAGCATGATGAATAT+CGG 0.354011 7.3:-62579394 MS.gene037224:CDS
GACTTTCTGGCGGCGCTTAG+TGG 0.354484 7.3:+62579519 None:intergenic
GTGGTTCTTGACGGAGTTCT+CGG 0.386546 7.3:+62579549 None:intergenic
ACAAGTGGGTTTGGATCAAA+TGG 0.404523 7.3:-62579256 MS.gene037224:CDS
GATGGAATGACAAGTGGGTT+TGG 0.405842 7.3:-62579265 MS.gene037224:CDS
GAAATTGCAAGGAGATTGTC+GGG 0.423852 7.3:-62579577 MS.gene037224:CDS
GGCGTTCCAGTGGTTCTTGA+CGG 0.436794 7.3:+62579540 None:intergenic
TCATCATCATTGAACATCAT+TGG 0.444693 7.3:+62579286 None:intergenic
TCCTGAAATTCCTATAAAGC+AGG 0.453856 7.3:-62579221 MS.gene037224:CDS
TTCTGCTACGAAAGTTGATA+AGG 0.456367 7.3:-62579431 MS.gene037224:CDS
ATCTGAGCATGATGAATATC+GGG 0.464340 7.3:-62579393 MS.gene037224:CDS
GGAAATTGCAAGGAGATTGT+CGG 0.469088 7.3:-62579578 MS.gene037224:CDS
ACCTTAAGAGTTTCTATATG+AGG 0.481236 7.3:+62579191 None:intergenic
ATCGAAGCTCACAAGATAGT+TGG 0.496519 7.3:-62579613 MS.gene037224:CDS
GAAAGTCGAGAAGAAGAATC+GGG 0.504233 7.3:-62579503 MS.gene037224:CDS
AGAAAGTCGAGAAGAAGAAT+CGG 0.513201 7.3:-62579504 MS.gene037224:CDS
AATGATTGAAGAAGGAGTCA+TGG 0.525168 7.3:-62579660 MS.gene037224:intron
GAATCGGGGTAACAGTTCAA+AGG 0.553014 7.3:-62579488 MS.gene037224:CDS
AATCGGGGTAACAGTTCAAA+GGG 0.564615 7.3:-62579487 MS.gene037224:CDS
GGCGCTTAGTGGCGTTCCAG+TGG 0.575961 7.3:+62579530 None:intergenic
AGAACTCCGTCAAGAACCAC+TGG 0.577545 7.3:-62579546 MS.gene037224:CDS
TGGGTTTGGATCAAATGGAA+TGG 0.578195 7.3:-62579251 MS.gene037224:CDS
ACAAGATAGTTGGTAACAAG+TGG 0.585557 7.3:-62579603 MS.gene037224:CDS
ATGATGTTCAATGATGATGA+TGG 0.603439 7.3:-62579283 MS.gene037224:CDS
ACAAGTGGGCGGAAATTGCA+AGG 0.615105 7.3:-62579588 MS.gene037224:CDS
ACTCCAGAAGATGAAATTGG+TGG 0.615237 7.3:-62579316 MS.gene037224:CDS
GAAGGAGTCATGGACCGAAG+AGG 0.642730 7.3:-62579650 MS.gene037224:CDS
GATGATGATGGAATGACAAG+TGG 0.643214 7.3:-62579271 MS.gene037224:CDS
ATGATGATGGAATGACAAGT+GGG 0.653031 7.3:-62579270 MS.gene037224:CDS
CAAGATAGTTGGTAACAAGT+GGG 0.659165 7.3:-62579602 MS.gene037224:CDS
TCTTCTTCTCGACTTTCTGG+CGG 0.690126 7.3:+62579509 None:intergenic
GATAGTTGGTAACAAGTGGG+CGG 0.706945 7.3:-62579599 MS.gene037224:CDS
AAAGTCGAGAAGAAGAATCG+GGG 0.733429 7.3:-62579502 MS.gene037224:CDS
AAATTGCAAGGAGATTGTCG+GGG 0.751711 7.3:-62579576 MS.gene037224:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATTAATTCACCAATT+TGG + chr7.3:62579281-62579300 None:intergenic 15.0%
!! CATTCAAAAAAGTTAATAAA+TGG + chr7.3:62579322-62579341 None:intergenic 15.0%
!! TATAATTAAAATAGTGTACA+AGG - chr7.3:62579224-62579243 MS.gene037224:CDS 15.0%
!!! ACTACTTTTGAAATACATAA+TGG - chr7.3:62579537-62579556 MS.gene037224:CDS 20.0%
! AGAATAAGCTATTACCAAAT+TGG - chr7.3:62579264-62579283 MS.gene037224:CDS 25.0%
!!! CTTTTTTGAATGATTGAAGA+AGG - chr7.3:62579330-62579349 MS.gene037224:CDS 25.0%
CTCTCAGAACTTTCATAATT+TGG + chr7.3:62579637-62579656 None:intergenic 30.0%
TATCTGAGCATGATGAATAT+CGG - chr7.3:62579604-62579623 MS.gene037224:CDS 30.0%
TCATCATCATTGAACATCAT+TGG + chr7.3:62579715-62579734 None:intergenic 30.0%
! ATGATGTTCAATGATGATGA+TGG - chr7.3:62579715-62579734 MS.gene037224:intron 30.0%
! CAATATTTTGTCTTCCTCTT+CGG + chr7.3:62579365-62579384 None:intergenic 30.0%
AATGATTGAAGAAGGAGTCA+TGG - chr7.3:62579338-62579357 MS.gene037224:CDS 35.0%
ACTACTCCAGAAGATGAAAT+TGG - chr7.3:62579679-62579698 MS.gene037224:intron 35.0%
AGAAAGTCGAGAAGAAGAAT+CGG - chr7.3:62579494-62579513 MS.gene037224:CDS 35.0%
AGGTCAATTTCCTGCTTTAT+AGG + chr7.3:62579790-62579809 None:intergenic 35.0%
ATCTGAGCATGATGAATATC+GGG - chr7.3:62579605-62579624 MS.gene037224:CDS 35.0%
TCCTGAAATTCCTATAAAGC+AGG - chr7.3:62579777-62579796 MS.gene037224:intron 35.0%
TCCTGCTTTATAGGAATTTC+AGG + chr7.3:62579781-62579800 None:intergenic 35.0%
!! ACAAGATAGTTGGTAACAAG+TGG - chr7.3:62579395-62579414 MS.gene037224:CDS 35.0%
!! ATGATGATGGAATGACAAGT+GGG - chr7.3:62579728-62579747 MS.gene037224:intron 35.0%
!! CAAGATAGTTGGTAACAAGT+GGG - chr7.3:62579396-62579415 MS.gene037224:CDS 35.0%
!! TTCTGCTACGAAAGTTGATA+AGG - chr7.3:62579567-62579586 MS.gene037224:CDS 35.0%
!!! GAGAGTGATTTTTTCTCTGA+AGG - chr7.3:62579652-62579671 MS.gene037224:CDS 35.0%
AAAGTCGAGAAGAAGAATCG+GGG - chr7.3:62579496-62579515 MS.gene037224:CDS 40.0%
AAATTGCAAGGAGATTGTCG+GGG - chr7.3:62579422-62579441 MS.gene037224:CDS 40.0%
AATCGGGGTAACAGTTCAAA+GGG - chr7.3:62579511-62579530 MS.gene037224:CDS 40.0%
ACTCCAGAAGATGAAATTGG+TGG - chr7.3:62579682-62579701 MS.gene037224:intron 40.0%
ATCGAAGCTCACAAGATAGT+TGG - chr7.3:62579385-62579404 MS.gene037224:CDS 40.0%
GAAAGTCGAGAAGAAGAATC+GGG - chr7.3:62579495-62579514 MS.gene037224:CDS 40.0%
GAAATTGCAAGGAGATTGTC+GGG - chr7.3:62579421-62579440 MS.gene037224:CDS 40.0%
GATTCTTCTTCTCGACTTTC+TGG + chr7.3:62579495-62579514 None:intergenic 40.0%
GGAAATTGCAAGGAGATTGT+CGG - chr7.3:62579420-62579439 MS.gene037224:CDS 40.0%
TAGCCACCAATTTCATCTTC+TGG + chr7.3:62579688-62579707 None:intergenic 40.0%
TGGGTTTGGATCAAATGGAA+TGG - chr7.3:62579747-62579766 MS.gene037224:intron 40.0%
! ACAAGTGGGTTTGGATCAAA+TGG - chr7.3:62579742-62579761 MS.gene037224:intron 40.0%
!! GATGATGATGGAATGACAAG+TGG - chr7.3:62579727-62579746 MS.gene037224:intron 40.0%
GAATCGGGGTAACAGTTCAA+AGG - chr7.3:62579510-62579529 MS.gene037224:CDS 45.0%
TCTTCTTCTCGACTTTCTGG+CGG + chr7.3:62579492-62579511 None:intergenic 45.0%
! GATGGAATGACAAGTGGGTT+TGG - chr7.3:62579733-62579752 MS.gene037224:intron 45.0%
!! GATAGTTGGTAACAAGTGGG+CGG - chr7.3:62579399-62579418 MS.gene037224:CDS 45.0%
ACAAGTGGGCGGAAATTGCA+AGG - chr7.3:62579410-62579429 MS.gene037224:CDS 50.0%
AGAACTCCGTCAAGAACCAC+TGG - chr7.3:62579452-62579471 MS.gene037224:CDS 50.0%
GTGGTTCTTGACGGAGTTCT+CGG + chr7.3:62579452-62579471 None:intergenic 50.0%
GAAGGAGTCATGGACCGAAG+AGG - chr7.3:62579348-62579367 MS.gene037224:CDS 55.0%
GGCGTTCCAGTGGTTCTTGA+CGG + chr7.3:62579461-62579480 None:intergenic 55.0%
! GACTTTCTGGCGGCGCTTAG+TGG + chr7.3:62579482-62579501 None:intergenic 60.0%
GGCGCTTAGTGGCGTTCCAG+TGG + chr7.3:62579471-62579490 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 62579204 62579816 62579204 ID=MS.gene037224
chr7.3 mRNA 62579204 62579816 62579204 ID=MS.gene037224.t1;Parent=MS.gene037224
chr7.3 exon 62579805 62579816 62579805 ID=MS.gene037224.t1.exon1;Parent=MS.gene037224.t1
chr7.3 CDS 62579805 62579816 62579805 ID=cds.MS.gene037224.t1;Parent=MS.gene037224.t1
chr7.3 exon 62579204 62579671 62579204 ID=MS.gene037224.t1.exon2;Parent=MS.gene037224.t1
chr7.3 CDS 62579204 62579671 62579204 ID=cds.MS.gene037224.t1;Parent=MS.gene037224.t1
Gene Sequence

>MS.gene037224

ATGCAATGTCTAGTTAGATATATAATTAAAATAGTGTACAAGGATGCAAAACTTTACTTGAGAATAAGCTATTACCAAATTGGTGAATTAATTTAATCATATTTATATAACTAATCCATTTATTAACTTTTTTGAATGATTGAAGAAGGAGTCATGGACCGAAGAGGAAGACAAAATATTGATCGAAGCTCACAAGATAGTTGGTAACAAGTGGGCGGAAATTGCAAGGAGATTGTCGGGGCGCACCGAGAACTCCGTCAAGAACCACTGGAACGCCACTAAGCGCCGCCAGAAAGTCGAGAAGAAGAATCGGGGTAACAGTTCAAAGGGAACACTACTTTTGAAATACATAATGGAAGTCACTTCTGCTACGAAAGTTGATAAGGAAATGATGAATAATTATCTGAGCATGATGAATATCGGGAATCAGCCAAATTATGAAAGTTCTGAGAGTGATTTTTTCTCTGAAGGTTTGACTACTCCAGAAGATGAAATTGGTGGCTATGTGCCAATGATGTTCAATGATGATGATGGAATGACAAGTGGGTTTGGATCAAATGGAATGGAGTTTTTTCCTGAAATTCCTATAAAGCAGGAAATTGACCTCATATAG

Protein sequence

>MS.gene037224.t1

MQCLKESWTEEEDKILIEAHKIVGNKWAEIARRLSGRTENSVKNHWNATKRRQKVEKKNRGNSSKGTLLLKYIMEVTSATKVDKEMMNNYLSMMNIGNQPNYESSESDFFSEGLTTPEDEIGGYVPMMFNDDDGMTSGFGSNGMEFFPEIPIKQEIDLI