Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037283.t1 | XP_003601199.1 | 57.6 | 177 | 35 | 11 | 43 | 197 | 191 | 349 | 5.50E-31 | 144.4 |
| MS.gene037283.t1 | XP_003601199.1 | 90.9 | 44 | 4 | 0 | 1 | 44 | 1 | 44 | 4.70E-14 | 88.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037283.t1 | Q8LPH6 | 79.5 | 44 | 9 | 0 | 1 | 44 | 1 | 44 | 2.6e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037283.t1 | G7J4T2 | 57.6 | 177 | 35 | 11 | 43 | 197 | 191 | 349 | 4.0e-31 | 144.4 |
| MS.gene037283.t1 | G7J4T2 | 90.9 | 44 | 4 | 0 | 1 | 44 | 1 | 44 | 3.4e-14 | 88.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene037283.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037283.t1 | MTR_3g077110 | 57.062 | 177 | 36 | 11 | 43 | 197 | 191 | 349 | 4.25e-41 | 142 |
| MS.gene037283.t1 | MTR_3g077110 | 85.417 | 48 | 7 | 0 | 1 | 48 | 1 | 48 | 1.62e-21 | 90.5 |
| MS.gene037283.t1 | MTR_1g112760 | 89.130 | 46 | 5 | 0 | 3 | 48 | 4 | 49 | 6.41e-22 | 89.7 |
| MS.gene037283.t1 | MTR_4g105130 | 79.167 | 48 | 10 | 0 | 1 | 48 | 1 | 48 | 1.48e-19 | 85.1 |
| MS.gene037283.t1 | MTR_4g105130 | 35.204 | 196 | 79 | 14 | 43 | 197 | 173 | 361 | 2.50e-11 | 62.0 |
| MS.gene037283.t1 | MTR_1g085040 | 35.714 | 154 | 76 | 4 | 1 | 135 | 1 | 150 | 1.56e-17 | 79.7 |
| MS.gene037283.t1 | MTR_7g111290 | 75.000 | 48 | 12 | 0 | 1 | 48 | 1 | 48 | 1.72e-17 | 79.7 |
| MS.gene037283.t1 | MTR_7g110830 | 75.000 | 44 | 11 | 0 | 1 | 44 | 1 | 44 | 1.71e-16 | 76.6 |
| MS.gene037283.t1 | MTR_8g017480 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 5.50e-16 | 69.3 |
| MS.gene037283.t1 | MTR_1g085640 | 72.727 | 44 | 12 | 0 | 1 | 44 | 1 | 44 | 8.73e-16 | 74.7 |
| MS.gene037283.t1 | MTR_2g099740 | 66.667 | 48 | 16 | 0 | 1 | 48 | 1 | 48 | 1.19e-15 | 74.7 |
| MS.gene037283.t1 | MTR_8g017500 | 64.583 | 48 | 17 | 0 | 1 | 48 | 1 | 48 | 3.62e-15 | 72.8 |
| MS.gene037283.t1 | MTR_8g017440 | 64.583 | 48 | 17 | 0 | 1 | 48 | 1 | 48 | 5.05e-15 | 72.4 |
| MS.gene037283.t1 | MTR_8g017540 | 64.583 | 48 | 17 | 0 | 1 | 48 | 47 | 94 | 5.53e-15 | 72.8 |
| MS.gene037283.t1 | MTR_8g017350 | 59.184 | 49 | 19 | 1 | 1 | 48 | 1 | 49 | 3.23e-12 | 64.7 |
| MS.gene037283.t1 | MTR_5g010020 | 56.250 | 48 | 21 | 0 | 1 | 48 | 1 | 48 | 3.63e-12 | 64.3 |
| MS.gene037283.t1 | MTR_8g017340 | 59.184 | 49 | 19 | 1 | 1 | 48 | 1 | 49 | 4.54e-12 | 64.3 |
| MS.gene037283.t1 | MTR_8g098860 | 56.250 | 48 | 21 | 0 | 1 | 48 | 1 | 48 | 6.12e-12 | 63.5 |
| MS.gene037283.t1 | MTR_8g017390 | 59.184 | 49 | 19 | 1 | 1 | 48 | 1 | 49 | 1.06e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037283.t1 | AT5G26660 | 75.000 | 48 | 12 | 0 | 1 | 48 | 1 | 48 | 7.16e-18 | 80.5 |
| MS.gene037283.t1 | AT1G57560 | 79.545 | 44 | 9 | 0 | 1 | 44 | 1 | 44 | 2.13e-17 | 79.0 |
| MS.gene037283.t1 | AT1G57560 | 79.545 | 44 | 9 | 0 | 1 | 44 | 1 | 44 | 2.13e-17 | 78.2 |
| MS.gene037283.t1 | AT4G01680 | 51.316 | 76 | 36 | 1 | 1 | 76 | 1 | 75 | 2.72e-17 | 79.0 |
| MS.gene037283.t1 | AT4G01680 | 75.000 | 44 | 11 | 0 | 1 | 44 | 1 | 44 | 1.06e-16 | 75.9 |
| MS.gene037283.t1 | AT4G01680 | 75.000 | 44 | 11 | 0 | 1 | 44 | 1 | 44 | 2.93e-16 | 75.9 |
| MS.gene037283.t1 | AT1G09540 | 75.000 | 44 | 11 | 0 | 1 | 44 | 1 | 44 | 9.93e-16 | 74.7 |
| MS.gene037283.t1 | AT3G13890 | 62.500 | 48 | 18 | 0 | 1 | 48 | 1 | 48 | 8.25e-14 | 69.3 |
| MS.gene037283.t1 | AT3G13890 | 65.909 | 44 | 15 | 0 | 1 | 44 | 1 | 44 | 1.16e-13 | 68.9 |
| MS.gene037283.t1 | AT1G63910 | 56.250 | 48 | 21 | 0 | 1 | 48 | 1 | 48 | 4.64e-12 | 64.3 |
Find 29 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATACAGTTCATAATAAT+TGG | 0.135565 | 1.1:-79809808 | None:intergenic |
| CACCAAAGGAGAACTAAATT+TGG | 0.229289 | 1.1:-79809546 | None:intergenic |
| AAAAGCTTCTTATCTTCTTC+AGG | 0.239901 | 1.1:-79808403 | None:intergenic |
| TTCAATTACATAACCATGTT+TGG | 0.314366 | 1.1:+79808424 | MS.gene037283:CDS |
| TCTTCTGACAGAGATGTTAA+AGG | 0.315827 | 1.1:-79809746 | None:intergenic |
| TTAAGATTACCAGCTAGTTT+AGG | 0.344561 | 1.1:-79808469 | None:intergenic |
| AAATACAGTTCATAATAATT+GGG | 0.349576 | 1.1:-79809807 | None:intergenic |
| TTCCAAATTTAGTTCTCCTT+TGG | 0.366592 | 1.1:+79809544 | MS.gene037283:CDS |
| AAGGCATTGAAGAGAAGAAT+TGG | 0.404191 | 1.1:-79809484 | None:intergenic |
| CATCAACAAGACAGATTCAA+TGG | 0.439637 | 1.1:+79809709 | MS.gene037283:CDS |
| TTCACTTCAAACTGCAACAA+AGG | 0.467959 | 1.1:-79809662 | None:intergenic |
| TACATAACCATGTTTGGTGT+TGG | 0.474556 | 1.1:+79808430 | MS.gene037283:CDS |
| AAAGCTTCTTATCTTCTTCA+GGG | 0.482620 | 1.1:-79808402 | None:intergenic |
| CCATGTTTGGTGTTGGATGT+TGG | 0.512330 | 1.1:+79808437 | MS.gene037283:CDS |
| TGAAAATTACAATAAGCTGT+TGG | 0.514835 | 1.1:+79809637 | MS.gene037283:CDS |
| AGTTCAGTTCCTAAACTAGC+TGG | 0.517760 | 1.1:+79808460 | MS.gene037283:CDS |
| GATTAATGAAAATGCCTCAT+GGG | 0.535807 | 1.1:+79809610 | MS.gene037283:CDS |
| CCAACATCCAACACCAAACA+TGG | 0.541756 | 1.1:-79808437 | None:intergenic |
| AATGGATCAAAATCAAATGT+TGG | 0.545963 | 1.1:-79809386 | None:intergenic |
| AATTGTAATTGACTTGGCTG+AGG | 0.553273 | 1.1:-79809449 | None:intergenic |
| ATACTTAAATTGTAGTGCAA+TGG | 0.557404 | 1.1:-79809404 | None:intergenic |
| TGATTAATGAAAATGCCTCA+TGG | 0.558714 | 1.1:+79809609 | MS.gene037283:CDS |
| ACTTCAAATTGTAATTGACT+TGG | 0.559630 | 1.1:-79809455 | None:intergenic |
| TGACAGAGATGTTAAAGGAT+AGG | 0.564100 | 1.1:-79809741 | None:intergenic |
| AATGAAAATGCCTCATGGGA+AGG | 0.570813 | 1.1:+79809614 | MS.gene037283:CDS |
| AATACAGTTCATAATAATTG+GGG | 0.584426 | 1.1:-79809806 | None:intergenic |
| AAGCTTCTTATCTTCTTCAG+GGG | 0.610649 | 1.1:-79808401 | None:intergenic |
| TCACTTTCAATATTCACCAA+AGG | 0.621840 | 1.1:-79809560 | None:intergenic |
| TTGTCTGAACTATTTAGACA+AGG | 0.673798 | 1.1:-79809503 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTAATCACTATAATAT+TGG | + | chr1.1:79809314-79809333 | MS.gene037283:intron | 10.0% |
| !! | TCATAAAAATTATATATGCA+TGG | - | chr1.1:79808801-79808820 | None:intergenic | 15.0% |
| !!! | ATAATGAGATAAAAAACTTT+TGG | + | chr1.1:79809081-79809100 | MS.gene037283:intron | 15.0% |
| !! | AAAAACTAAGAAAAGGTTTA+TGG | + | chr1.1:79808377-79808396 | MS.gene037283:CDS | 20.0% |
| !! | AAATTATATATGCATGGTAA+AGG | - | chr1.1:79808795-79808814 | None:intergenic | 20.0% |
| !! | ATGGTAAAGGAAAAAATTTA+AGG | - | chr1.1:79808782-79808801 | None:intergenic | 20.0% |
| !! | ATTAATCACTATAATATTGG+TGG | + | chr1.1:79809317-79809336 | MS.gene037283:intron | 20.0% |
| !! | CAATAATGAATCACTTATAA+AGG | + | chr1.1:79809169-79809188 | MS.gene037283:intron | 20.0% |
| !! | TTAAAGCAAAAACTAAGAAA+AGG | + | chr1.1:79808370-79808389 | MS.gene037283:CDS | 20.0% |
| !!! | ATAGTTGCTATATATTTTGT+TGG | - | chr1.1:79808545-79808564 | None:intergenic | 20.0% |
| !!! | ATATAATTTTTATGAGTTGC+AGG | + | chr1.1:79808806-79808825 | MS.gene037283:intron | 20.0% |
| !!! | TAGTTGCTATATATTTTGTT+GGG | - | chr1.1:79808544-79808563 | None:intergenic | 20.0% |
| !!! | TAGTTTTTGCTTTAAAGAAT+AGG | - | chr1.1:79808365-79808384 | None:intergenic | 20.0% |
| !!! | TATAATTTTTATGAGTTGCA+GGG | + | chr1.1:79808807-79808826 | MS.gene037283:intron | 20.0% |
| ! | AAGAAGAAACTAATGAAACA+AGG | + | chr1.1:79809116-79809135 | MS.gene037283:intron | 25.0% |
| ! | AAGTGATTCATTATTGATCA+AGG | - | chr1.1:79809165-79809184 | None:intergenic | 25.0% |
| ! | AATGGATCAAAATCAAATGT+TGG | - | chr1.1:79809389-79809408 | None:intergenic | 25.0% |
| ! | ACTTCAAATTGTAATTGACT+TGG | - | chr1.1:79809458-79809477 | None:intergenic | 25.0% |
| ! | AGAAGAAACTAATGAAACAA+GGG | + | chr1.1:79809117-79809136 | MS.gene037283:intron | 25.0% |
| ! | AGTGATTCATTATTGATCAA+GGG | - | chr1.1:79809164-79809183 | None:intergenic | 25.0% |
| ! | ATACTTAAATTGTAGTGCAA+TGG | - | chr1.1:79809407-79809426 | None:intergenic | 25.0% |
| ! | CATATCAAGTTATCAACATA+TGG | + | chr1.1:79808721-79808740 | MS.gene037283:intron | 25.0% |
| ! | TGAAAATTACAATAAGCTGT+TGG | + | chr1.1:79809637-79809656 | MS.gene037283:CDS | 25.0% |
| ! | TTCAATTACATAACCATGTT+TGG | + | chr1.1:79808424-79808443 | MS.gene037283:CDS | 25.0% |
| AAAAGCTTCTTATCTTCTTC+AGG | - | chr1.1:79808406-79808425 | None:intergenic | 30.0% | |
| AAAGCTTCTTATCTTCTTCA+GGG | - | chr1.1:79808405-79808424 | None:intergenic | 30.0% | |
| ATCACTTATAAAGGAAGAGA+AGG | + | chr1.1:79809178-79809197 | MS.gene037283:intron | 30.0% | |
| GATTAATGAAAATGCCTCAT+GGG | + | chr1.1:79809610-79809629 | MS.gene037283:CDS | 30.0% | |
| TCAAATTGCATCACAATTAC+CGG | + | chr1.1:79809049-79809068 | MS.gene037283:intron | 30.0% | |
| TCACTTTCAATATTCACCAA+AGG | - | chr1.1:79809563-79809582 | None:intergenic | 30.0% | |
| TGAGATGGATAAACTATTTG+AGG | + | chr1.1:79808858-79808877 | MS.gene037283:intron | 30.0% | |
| TGATTAATGAAAATGCCTCA+TGG | + | chr1.1:79809609-79809628 | MS.gene037283:CDS | 30.0% | |
| TTAATTTCAAATGATGCCTC+AGG | + | chr1.1:79809021-79809040 | MS.gene037283:intron | 30.0% | |
| TTGTCTGAACTATTTAGACA+AGG | - | chr1.1:79809506-79809525 | None:intergenic | 30.0% | |
| ! | TTAAGATTACCAGCTAGTTT+AGG | - | chr1.1:79808472-79808491 | None:intergenic | 30.0% |
| ! | TTGGGTTGTACAAGTATATA+TGG | - | chr1.1:79808526-79808545 | None:intergenic | 30.0% |
| !! | TTCCAAATTTAGTTCTCCTT+TGG | + | chr1.1:79809544-79809563 | MS.gene037283:CDS | 30.0% |
| AAATTGCATCACAATTACCG+GGG | + | chr1.1:79809051-79809070 | MS.gene037283:intron | 35.0% | |
| AAGCTTCTTATCTTCTTCAG+GGG | - | chr1.1:79808404-79808423 | None:intergenic | 35.0% | |
| AGGAATGTTCTCAAAACAAG+AGG | + | chr1.1:79808895-79808914 | MS.gene037283:intron | 35.0% | |
| ATGGCATTCATGCATGTTTA+TGG | - | chr1.1:79808507-79808526 | None:intergenic | 35.0% | |
| CAAATTGCATCACAATTACC+GGG | + | chr1.1:79809050-79809069 | MS.gene037283:intron | 35.0% | |
| CACCAAAGGAGAACTAAATT+TGG | - | chr1.1:79809549-79809568 | None:intergenic | 35.0% | |
| CATCAACAAGACAGATTCAA+TGG | + | chr1.1:79809709-79809728 | MS.gene037283:CDS | 35.0% | |
| GAGAGAATGAAAGTGAGAAT+AGG | - | chr1.1:79808644-79808663 | None:intergenic | 35.0% | |
| GGATCATCATCTTTAACAGA+AGG | + | chr1.1:79809338-79809357 | MS.gene037283:intron | 35.0% | |
| TCTTCTGACAGAGATGTTAA+AGG | - | chr1.1:79809749-79809768 | None:intergenic | 35.0% | |
| TGACAGAGATGTTAAAGGAT+AGG | - | chr1.1:79809744-79809763 | None:intergenic | 35.0% | |
| TGCTTCTGAATATTCATGTG+AGG | - | chr1.1:79809279-79809298 | None:intergenic | 35.0% | |
| TTCACTTCAAACTGCAACAA+AGG | - | chr1.1:79809665-79809684 | None:intergenic | 35.0% | |
| ! | TTCATGAAGCACTTGGAAAT+AGG | + | chr1.1:79808936-79808955 | MS.gene037283:intron | 35.0% |
| !! | AAGGCATTGAAGAGAAGAAT+TGG | - | chr1.1:79809487-79809506 | None:intergenic | 35.0% |
| !! | AATTGTAATTGACTTGGCTG+AGG | - | chr1.1:79809452-79809471 | None:intergenic | 35.0% |
| !! | TACATAACCATGTTTGGTGT+TGG | + | chr1.1:79808430-79808449 | MS.gene037283:CDS | 35.0% |
| AACATTCCTCTCTTCAAACC+AGG | - | chr1.1:79808884-79808903 | None:intergenic | 40.0% | |
| AATGAAAATGCCTCATGGGA+AGG | + | chr1.1:79809614-79809633 | MS.gene037283:CDS | 40.0% | |
| AGTTCAGTTCCTAAACTAGC+TGG | + | chr1.1:79808460-79808479 | MS.gene037283:CDS | 40.0% | |
| ATCAAGGGTTTATGTGTAGC+TGG | - | chr1.1:79809149-79809168 | None:intergenic | 40.0% | |
| ATCTCATTATCTGTTCTCCC+CGG | - | chr1.1:79809071-79809090 | None:intergenic | 40.0% | |
| ATTTCAAATGATGCCTCAGG+TGG | + | chr1.1:79809024-79809043 | MS.gene037283:intron | 40.0% | |
| GAAAGAGCTGTAGATTGAGA+TGG | + | chr1.1:79808843-79808862 | MS.gene037283:intron | 40.0% | |
| GACAGTGGCATTATCATTGA+TGG | - | chr1.1:79809209-79809228 | None:intergenic | 40.0% | |
| TGTCTCAATCTCAACCTCAA+AGG | + | chr1.1:79809225-79809244 | MS.gene037283:intron | 40.0% | |
| TGTGTAGCTAAAGTCCTTTG+AGG | - | chr1.1:79809242-79809261 | None:intergenic | 40.0% | |
| TTTCAAATGATGCCTCAGGT+GGG | + | chr1.1:79809025-79809044 | MS.gene037283:intron | 40.0% | |
| ! | GTAATTTTCACCTTCCCATG+AGG | - | chr1.1:79809627-79809646 | None:intergenic | 40.0% |
| ! | GTCAAACTTCATGAAGCACT+TGG | + | chr1.1:79808929-79808948 | MS.gene037283:intron | 40.0% |
| ! | TGGATAAACTATTTGAGGCC+TGG | + | chr1.1:79808863-79808882 | MS.gene037283:intron | 40.0% |
| CCAACATCCAACACCAAACA+TGG | - | chr1.1:79808440-79808459 | None:intergenic | 45.0% | |
| TATGAGTTGCAGGGTTGCAA+AGG | + | chr1.1:79808816-79808835 | MS.gene037283:intron | 45.0% | |
| TGAGGTTGAGATTGAGACAG+TGG | - | chr1.1:79809224-79809243 | None:intergenic | 45.0% | |
| !! | CCATGTTTGGTGTTGGATGT+TGG | + | chr1.1:79808437-79808456 | MS.gene037283:CDS | 45.0% |
| ATGCAATTTGAGCCCACCTG+AGG | - | chr1.1:79809040-79809059 | None:intergenic | 50.0% | |
| ! | TTGAGGCCTGGTTTGAAGAG+AGG | + | chr1.1:79808875-79808894 | MS.gene037283:intron | 50.0% |
| GTTGCAGGGTTGCAAAGGTG+CGG | + | chr1.1:79808821-79808840 | MS.gene037283:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 79808349 | 79809820 | 79808349 | ID=MS.gene037283 |
| chr1.1 | mRNA | 79808349 | 79809820 | 79808349 | ID=MS.gene037283.t1;Parent=MS.gene037283 |
| chr1.1 | exon | 79808349 | 79808481 | 79808349 | ID=MS.gene037283.t1.exon1;Parent=MS.gene037283.t1 |
| chr1.1 | CDS | 79808349 | 79808481 | 79808349 | ID=cds.MS.gene037283.t1;Parent=MS.gene037283.t1 |
| chr1.1 | exon | 79809357 | 79809820 | 79809357 | ID=MS.gene037283.t1.exon2;Parent=MS.gene037283.t1 |
| chr1.1 | CDS | 79809357 | 79809820 | 79809357 | ID=cds.MS.gene037283.t1;Parent=MS.gene037283.t1 |
| Gene Sequence |
| Protein sequence |
