AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037335

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037335.t1 MTR_1g114070 95.050 707 35 0 2 708 218 924 0.0 1389
MS.gene037335.t1 MTR_1g114150 73.816 718 174 5 2 708 336 1050 0.0 1092
MS.gene037335.t1 MTR_1g114130 73.464 716 176 6 6 708 324 1038 0.0 1074
MS.gene037335.t1 MTR_1g069565 69.241 738 189 4 9 708 144 881 0.0 1034
MS.gene037335.t1 MTR_5g065200 69.817 709 205 3 2 708 133 834 0.0 998
MS.gene037335.t1 MTR_1g069565 63.396 795 196 7 9 708 144 938 0.0 981
MS.gene037335.t1 MTR_1g114150 72.982 607 150 5 2 597 336 939 0.0 907
MS.gene037335.t1 MTR_5g047620 63.063 666 209 8 73 708 139 797 0.0 826
MS.gene037335.t1 MTR_0020s0100 60.413 629 221 10 73 691 81 691 0.0 745
MS.gene037335.t1 MTR_2g024270 46.591 704 330 11 9 706 154 817 0.0 586
MS.gene037335.t1 MTR_4g132540 43.555 737 337 14 9 706 168 864 0.0 580
MS.gene037335.t1 MTR_2g024140 44.681 705 330 13 9 706 34 685 0.0 557
MS.gene037335.t1 MTR_7g117445 46.909 550 270 9 3 537 134 676 7.34e-157 476
MS.gene037335.t1 MTR_7g117445 65.591 93 32 0 555 647 773 865 3.75e-33 137
MS.gene037335.t1 MTR_7g117445 46.909 550 270 9 3 537 134 676 1.93e-156 476
MS.gene037335.t1 MTR_7g117445 61.438 153 59 0 555 707 773 925 1.37e-55 205
MS.gene037335.t1 MTR_7g117445 46.909 550 270 9 3 537 134 676 2.29e-156 476
MS.gene037335.t1 MTR_7g117445 61.438 153 59 0 555 707 773 925 1.50e-55 205
MS.gene037335.t1 MTR_7g117445 46.909 550 270 9 3 537 134 676 7.47e-156 476
MS.gene037335.t1 MTR_7g117445 61.438 153 59 0 555 707 773 925 1.66e-55 205
MS.gene037335.t1 MTR_1g083120 34.987 746 399 11 3 707 192 892 6.58e-151 462
MS.gene037335.t1 MTR_1g083120 34.987 746 399 11 3 707 209 909 1.11e-150 462
MS.gene037335.t1 MTR_1g008060 45.386 531 250 10 9 533 861 1357 1.97e-132 429
MS.gene037335.t1 MTR_1g008060 68.382 136 43 0 562 697 1542 1677 1.33e-54 204
MS.gene037335.t1 MTR_1g008060 45.386 531 250 10 9 533 870 1366 2.45e-132 428
MS.gene037335.t1 MTR_1g008060 68.382 136 43 0 562 697 1551 1686 1.31e-54 204
MS.gene037335.t1 MTR_1g083120 34.192 699 373 12 3 659 192 845 1.03e-131 410
MS.gene037335.t1 MTR_1g083120 34.448 688 365 11 3 649 192 834 1.10e-131 410
MS.gene037335.t1 MTR_2g011630 34.305 755 416 15 9 698 207 946 2.99e-128 404
MS.gene037335.t1 MTR_8g062940 52.989 368 142 3 9 376 33 369 7.05e-122 369
MS.gene037335.t1 MTR_2g011630 34.770 673 359 14 91 698 77 734 8.52e-115 363
MS.gene037335.t1 MTR_2g011630 34.770 673 359 14 91 698 45 702 8.71e-115 362
MS.gene037335.t1 MTR_4g091520 36.250 560 265 13 231 707 727 1277 1.22e-100 336
MS.gene037335.t1 MTR_4g091520 37.143 175 100 4 9 174 260 433 7.17e-28 120
MS.gene037335.t1 MTR_2g024240 54.325 289 116 3 385 671 114 388 4.30e-99 310
MS.gene037335.t1 MTR_4g091520 41.892 296 163 5 231 523 657 946 1.33e-61 224
MS.gene037335.t1 MTR_4g091520 37.143 175 100 4 9 174 260 433 5.87e-28 121
MS.gene037335.t1 MTR_4g091520 41.892 296 163 5 231 523 692 981 1.88e-61 224
MS.gene037335.t1 MTR_4g091520 37.143 175 100 4 9 174 260 433 5.74e-28 121
MS.gene037335.t1 MTR_4g091520 41.892 296 163 5 231 523 727 1016 1.93e-61 224
MS.gene037335.t1 MTR_4g091520 37.143 175 100 4 9 174 260 433 6.38e-28 121
MS.gene037335.t1 MTR_0295s0020 59.659 176 66 2 375 547 11 184 7.79e-61 202
MS.gene037335.t1 MTR_8g465700 53.333 180 80 2 318 497 2 177 7.63e-54 187
MS.gene037335.t1 MTR_8g465720 67.416 89 28 1 561 649 52 139 1.23e-34 129
MS.gene037335.t1 MTR_8g013920 54.930 142 41 4 410 547 40 162 2.56e-32 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037335.t1 AT1G11950 48.442 706 336 11 4 706 188 868 0.0 640
MS.gene037335.t1 AT1G11950 48.442 706 336 11 4 706 188 868 0.0 640
MS.gene037335.t1 AT1G62310 49.062 693 331 10 8 695 208 883 0.0 634
MS.gene037335.t1 AT4G00990 42.139 776 353 14 5 706 76 829 0.0 580
MS.gene037335.t1 AT1G11950 47.261 639 309 11 4 639 188 801 0.0 553
MS.gene037335.t1 AT3G07610 56.994 479 195 4 221 698 415 883 3.11e-177 533
MS.gene037335.t1 AT3G07610 44.974 189 103 1 7 195 153 340 7.64e-47 179
MS.gene037335.t1 AT4G00990 40.828 725 333 14 5 655 76 778 1.97e-172 513
MS.gene037335.t1 AT4G00990 41.144 717 326 14 5 647 76 770 4.36e-172 512
MS.gene037335.t1 AT3G07610 54.618 498 199 5 221 698 415 905 3.76e-169 513
MS.gene037335.t1 AT3G07610 44.974 189 103 1 7 195 153 340 6.37e-47 180
MS.gene037335.t1 AT1G09060 32.473 736 422 12 3 707 200 891 3.47e-120 382
MS.gene037335.t1 AT1G09060 32.473 736 422 12 3 707 200 891 3.47e-120 382
MS.gene037335.t1 AT1G09060 32.473 736 422 12 3 707 214 905 4.12e-120 382
MS.gene037335.t1 AT1G09060 32.473 736 422 12 3 707 214 905 4.12e-120 382
MS.gene037335.t1 AT3G07610 56.890 283 115 2 221 503 415 690 3.17e-95 311
MS.gene037335.t1 AT3G07610 44.974 189 103 1 7 195 153 340 6.62e-47 178
MS.gene037335.t1 AT4G21430 34.642 433 244 5 306 705 485 911 2.10e-80 275
MS.gene037335.t1 AT4G21430 29.299 157 108 3 9 163 227 382 8.58e-12 69.3

Find 162 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAGATATTCTTTCAATT+TGG 0.093161 1.2:-84027720 None:intergenic
GCCGATTCTACCTGCTTTAA+AGG 0.132025 1.2:-84027779 None:intergenic
ATATCACTCTCTTTCAATTT+AGG 0.147719 1.2:-84023987 None:intergenic
GCCTCCTTCTGATTTATTTC+AGG 0.172247 1.2:+84026768 MS.gene037335:CDS
AACCAAAACTACATCGTATT+TGG 0.218834 1.2:-84025218 None:intergenic
AAATCATACTCAAGCTTAAT+TGG 0.220679 1.2:-84024653 None:intergenic
TCACATTCATTATCAGCTTT+TGG 0.248622 1.2:-84024815 None:intergenic
GGATTTGTGTATTCTTTATA+AGG 0.268369 1.2:-84026832 None:intergenic
GTCGCAGCGATTGGCTTAAA+TGG 0.273010 1.2:+84026719 MS.gene037335:CDS
AGTACATTGCTGACAATTAC+AGG 0.278275 1.2:-84025127 None:intergenic
CCAAGAACATATATTGCTTA+TGG 0.280860 1.2:+84026919 MS.gene037335:CDS
ATCCCAGAGAGCACCTTCTA+AGG 0.286251 1.2:-84027675 None:intergenic
TGTCATTTCTTTGACATTGA+TGG 0.286327 1.2:-84023698 None:intergenic
CTCCAAATACGATGTAGTTT+TGG 0.286573 1.2:+84025216 MS.gene037335:CDS
CTCCGTGGTGGGAAGCTTCT+AGG 0.287089 1.2:+84024620 MS.gene037335:CDS
TCTAGATATTCTTTCAATTT+GGG 0.290597 1.2:-84027719 None:intergenic
ACTAACTCAGAGATGCAATT+TGG 0.317312 1.2:-84024872 None:intergenic
TCAGACCATTTATCTGAATA+TGG 0.327236 1.2:+84028245 MS.gene037335:CDS
CCATAAGCAATATATGTTCT+TGG 0.328103 1.2:-84026919 None:intergenic
CAACCTTGCTGTGAAGTTGC+CGG 0.339593 1.2:+84026870 MS.gene037335:CDS
ATGCTCCATATTCAGATAAA+TGG 0.340188 1.2:-84028250 None:intergenic
ATGAAACTTAAGAAAGAGTA+TGG 0.363484 1.2:+84028276 MS.gene037335:CDS
ATTCGTGAGTGGTCAAGATC+TGG 0.381498 1.2:+84024758 MS.gene037335:CDS
ATCCCTTAGAAGGTGCTCTC+TGG 0.383194 1.2:+84027672 MS.gene037335:CDS
CTAAAGATGAGCGTGTGTTC+TGG 0.394804 1.2:+84024430 MS.gene037335:CDS
CTCAAAGCTAAATATAAAAC+CGG 0.396606 1.2:+84024398 MS.gene037335:CDS
CTCAACCTCCTCCTCGTTGT+TGG 0.396999 1.2:-84024191 None:intergenic
GTTCTTGCTCGAAACCTGTT+AGG 0.397008 1.2:+84024963 MS.gene037335:CDS
TAGAGGAGACTCGTGATAAT+AGG 0.397753 1.2:+84024939 MS.gene037335:CDS
ATATCATCGGTGTACCTAAC+AGG 0.407455 1.2:-84024977 None:intergenic
AGGCAGTTAATTGTTTAGAT+TGG 0.408112 1.2:+84025245 MS.gene037335:CDS
TTCAATGGCACTGGAGCAAA+GGG 0.413215 1.2:+84025101 MS.gene037335:CDS
TATGTTCTTGGCCCCATGTC+AGG 0.417723 1.2:-84026907 None:intergenic
AATATCCACCAATTCTTCAC+TGG 0.420686 1.2:+84026685 MS.gene037335:CDS
AGGTTGAGAGAACCACTAAA+AGG 0.429360 1.2:-84026853 None:intergenic
CGAGGTCGCAATTATCTACA+TGG 0.434006 1.2:+84024677 MS.gene037335:CDS
AATGTACTTGAATGCACATC+TGG 0.441844 1.2:+84025142 MS.gene037335:CDS
TTTGTCTCTCCCGAACATAT+TGG 0.442105 1.2:+84029835 MS.gene037335:CDS
GCAGCGGATCTGTGATTTAT+CGG 0.450090 1.2:-84029895 None:intergenic
GTTGGAGAGGATCCCTTAGA+AGG 0.451802 1.2:+84027662 MS.gene037335:CDS
GAAGCACTCACCAATATGTT+CGG 0.452411 1.2:-84029845 None:intergenic
TTCTTCACTGGGTACACAAA+TGG 0.460684 1.2:+84026697 MS.gene037335:CDS
TCTTCCACATTTAAGACGGT+TGG 0.461419 1.2:+84024240 MS.gene037335:CDS
GAAGTATCCTTCTCAGTTCA+AGG 0.464781 1.2:-84024847 None:intergenic
AGCACCTTGAGCAAGATAAG+AGG 0.465265 1.2:+84027567 MS.gene037335:CDS
TTAATGACACTAATTTCCTC+AGG 0.465562 1.2:-84027530 None:intergenic
TTACCTTCAGATTTCTGACT+TGG 0.467821 1.2:-84028464 None:intergenic
AATGCACATCTGGTTTAAGC+TGG 0.467845 1.2:+84025152 MS.gene037335:CDS
TCTTTCAATTTCAACACCTC+AGG 0.472947 1.2:-84026742 None:intergenic
ATTTGGAGTTGGTTATTTGA+CGG 0.476343 1.2:-84025201 None:intergenic
TTGTGTACCCAGTGAAGAAT+TGG 0.478196 1.2:-84026693 None:intergenic
GACTTGTAGGAATTGAGCCT+TGG 0.481982 1.2:+84028388 MS.gene037335:intron
ATCCGCTGCGGACAAGTTTG+AGG 0.482235 1.2:+84029909 MS.gene037335:CDS
ACACAAATGGTCGCAGCGAT+TGG 0.484713 1.2:+84026710 MS.gene037335:CDS
TCCCTTAGAAGGTGCTCTCT+GGG 0.498565 1.2:+84027673 MS.gene037335:CDS
ATCAAGTGCAACCTTGATGC+AGG 0.498619 1.2:-84029812 None:intergenic
CACATCAAGCATAGCATGTA+CGG 0.501504 1.2:-84030539 None:intergenic
ATCAATGTCAAAGAAATGAC+AGG 0.501804 1.2:+84023700 MS.gene037335:CDS
TGCGGAATTCCTCTGTCAAA+CGG 0.503979 1.2:-84029867 None:intergenic
GCACATCATGTATACAAAAG+TGG 0.504161 1.2:+84023766 MS.gene037335:CDS
AGGTGTTGAAATTGAAAGAT+TGG 0.508050 1.2:+84026746 MS.gene037335:CDS
AGGGATCCTCTCCAACCATC+AGG 0.508501 1.2:-84027655 None:intergenic
AACTACATCGTATTTGGAGT+TGG 0.509194 1.2:-84025212 None:intergenic
TTTCAATGGCACTGGAGCAA+AGG 0.515097 1.2:+84025100 MS.gene037335:CDS
GTTGACTCATATCGCTAAAG+TGG 0.516636 1.2:+84027499 MS.gene037335:CDS
TCAGGCTTTACACAATAATC+CGG 0.522487 1.2:-84026889 None:intergenic
CGCAGCAACAGAGGAGACAA+AGG 0.527535 1.2:-84024595 None:intergenic
AAGCACTCACCAATATGTTC+GGG 0.527844 1.2:-84029844 None:intergenic
GCTGATAATGAATGTGATCA+TGG 0.528933 1.2:+84024821 MS.gene037335:CDS
TCTGTTGCAGTCCTGCATCA+AGG 0.530126 1.2:+84029801 MS.gene037335:intron
TGAAGAACAAATGATTGAGA+AGG 0.530667 1.2:+84024264 MS.gene037335:CDS
AAGAGGGAGCTACATGGTGA+TGG 0.531116 1.2:+84027584 MS.gene037335:CDS
GTTGCTGCGAGCTCCGTGGT+GGG 0.531732 1.2:+84024609 MS.gene037335:CDS
GATAAATGGTCTGATCATGA+AGG 0.531871 1.2:-84028236 None:intergenic
AGAGGGAGCTACATGGTGAT+GGG 0.532113 1.2:+84027585 MS.gene037335:CDS
ATGGTTTCCTTGAACTGAGA+AGG 0.533086 1.2:+84024840 MS.gene037335:CDS
CAAACCAGCAGGAATGAAAA+CGG 0.534926 1.2:-84028436 None:intergenic
GCTACATGGTGATGGGGAGG+CGG 0.535164 1.2:+84027592 MS.gene037335:CDS
TGTGTATTCTTTATAAGGCA+AGG 0.535177 1.2:-84026827 None:intergenic
TATGGATTTACTCAAAATCT+CGG 0.535793 1.2:+84026937 MS.gene037335:CDS
TATAGGAATACCTCGTCTCC+TGG 0.539286 1.2:-84024296 None:intergenic
TGTGTTATTGTGTATATCAT+CGG 0.540704 1.2:-84024990 None:intergenic
TTCGTGAGTGGTCAAGATCT+GGG 0.542666 1.2:+84024759 MS.gene037335:CDS
CAATTGCATTCCTTTAAAGC+AGG 0.542700 1.2:+84027769 MS.gene037335:CDS
GCTCCCTCTTATCTTGCTCA+AGG 0.544578 1.2:-84027571 None:intergenic
ACTTGGTGAGGCAAACCAGC+AGG 0.547834 1.2:-84028447 None:intergenic
GCACCTTGAGCAAGATAAGA+GGG 0.549030 1.2:+84027568 MS.gene037335:CDS
TTAGATGCACAAAATGCAAA+CGG 0.550175 1.2:+84023733 MS.gene037335:CDS
TCCCAGAGAGCACCTTCTAA+GGG 0.550365 1.2:-84027674 None:intergenic
TCATCCAACCGTCTTAAATG+TGG 0.550657 1.2:-84024244 None:intergenic
AACTCAGAGATGCAATTTGG+AGG 0.551495 1.2:-84024869 None:intergenic
TGGTGTAGATCTACAGCCCT+TGG 0.555022 1.2:-84025061 None:intergenic
TTGACATTGATGGCACATTG+AGG 0.559359 1.2:-84023688 None:intergenic
TGTTGCTGCGAGCTCCGTGG+TGG 0.562600 1.2:+84024608 MS.gene037335:CDS
ATATCCACCAATTCTTCACT+GGG 0.564592 1.2:+84026686 MS.gene037335:CDS
TTCTCTAAGTATCTGCTGAA+AGG 0.564863 1.2:+84024214 MS.gene037335:CDS
TTCAGATTTCTGACTTGGTG+AGG 0.565040 1.2:-84028459 None:intergenic
GACTTCTTCCACATTTAAGA+CGG 0.566290 1.2:+84024236 MS.gene037335:CDS
TGTACCCAGTGAAGAATTGG+TGG 0.567688 1.2:-84026690 None:intergenic
ACAGCACACAGGGCATGCCT+TGG 0.575809 1.2:-84024012 None:intergenic
CAAGATAAGAGGGAGCTACA+TGG 0.577423 1.2:+84027578 MS.gene037335:CDS
ATGCACATCTGGTTTAAGCT+GGG 0.577804 1.2:+84025153 MS.gene037335:CDS
TGTCAAAGAAATGACAGGGC+CGG 0.578080 1.2:+84023705 MS.gene037335:CDS
CAACTCTCTGACACTAATGA+TGG 0.579184 1.2:+84027629 MS.gene037335:CDS
CACGGAGCTCGCAGCAACAG+AGG 0.580415 1.2:-84024604 None:intergenic
GGAGCTACATGGTGATGGGG+AGG 0.581361 1.2:+84027589 MS.gene037335:CDS
TGCAATTATCACAGCACACA+GGG 0.584315 1.2:-84024022 None:intergenic
ACTATAGGAAATGAAGCGTA+AGG 0.584966 1.2:+84024162 MS.gene037335:intron
ATAAATGGTCTGATCATGAA+GGG 0.587122 1.2:-84028235 None:intergenic
CATCATTAGTGTCAGAGAGT+TGG 0.587536 1.2:-84027628 None:intergenic
AATCAAGCTTCAAGATGTAG+AGG 0.589159 1.2:+84024922 MS.gene037335:CDS
TATTGTGTAAAGCCTGACAT+GGG 0.589216 1.2:+84026895 MS.gene037335:CDS
TGAAACCAACAACGAGGAGG+AGG 0.589775 1.2:+84024186 MS.gene037335:CDS
AGTTCCTGAAATAAATCAGA+AGG 0.590173 1.2:-84026772 None:intergenic
CACTAATGATGGTGACCTGA+TGG 0.592137 1.2:+84027640 MS.gene037335:CDS
TGGACAAGGAATGCTACCAT+CGG 0.593451 1.2:-84024795 None:intergenic
GCAATTGCAAAGCATGCTTG+CGG 0.593478 1.2:+84024042 MS.gene037335:CDS
TTGAGAAGGAAAGAGAAGCC+AGG 0.596035 1.2:+84024278 MS.gene037335:CDS
GAAAGAGAAGCCAGGAGACG+AGG 0.596795 1.2:+84024286 MS.gene037335:CDS
AATGATGGTGACCTGATGGT+TGG 0.598278 1.2:+84027644 MS.gene037335:CDS
AACTTCTGAATAAAAGTCCA+AGG 0.598579 1.2:-84028405 None:intergenic
AGAAAGTGTATCACATGCAG+AGG 0.606509 1.2:+84024724 MS.gene037335:CDS
CACCTAGAAGCTTCCCACCA+CGG 0.608942 1.2:-84024622 None:intergenic
TGTGATTTATCGGAAGCTTG+CGG 0.611620 1.2:-84029885 None:intergenic
TTATTGTGTAAAGCCTGACA+TGG 0.616770 1.2:+84026894 MS.gene037335:CDS
CTCTGTTGCTGCGAGCTCCG+TGG 0.620459 1.2:+84024605 MS.gene037335:CDS
TTGCAATTATCACAGCACAC+AGG 0.625183 1.2:-84024023 None:intergenic
GAAAGAGAGTGATATTGCCA+AGG 0.625820 1.2:+84023995 MS.gene037335:CDS
TCCTGAAATAAATCAGAAGG+AGG 0.633909 1.2:-84026769 None:intergenic
TATACACAATAACACAAGGA+AGG 0.636388 1.2:+84024997 MS.gene037335:CDS
TCACCAAGTCAGAAATCTGA+AGG 0.640211 1.2:+84028461 MS.gene037335:CDS
CGTACATGCTATGCTTGATG+TGG 0.642539 1.2:+84030540 MS.gene037335:CDS
AACCTCAAACTTGTCCGCAG+CGG 0.644100 1.2:-84029911 None:intergenic
AATGCACGCCACATGACTAG+TGG 0.646851 1.2:-84025178 None:intergenic
TCATCTTTAGAATAATCCGC+CGG 0.653440 1.2:-84024417 None:intergenic
GAGGGAGCTACATGGTGATG+GGG 0.656552 1.2:+84027586 MS.gene037335:CDS
CGTGGTGGGAAGCTTCTAGG+TGG 0.658159 1.2:+84024623 MS.gene037335:CDS
TAATTGTTTAGATTGGTGTG+AGG 0.659042 1.2:+84025252 MS.gene037335:CDS
TAAATGGTCTGATCATGAAG+GGG 0.660847 1.2:-84028234 None:intergenic
AAAGCTAAATATAAAACCGG+CGG 0.661902 1.2:+84024401 MS.gene037335:CDS
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 1.2:+84029858 MS.gene037335:CDS
AATCCGGCAACTTCACAGCA+AGG 0.666731 1.2:-84026873 None:intergenic
TAAAGTGGAACTAAAACCTG+AGG 0.669552 1.2:+84027514 MS.gene037335:CDS
CGATTGGCTTAAATGGCCTG+AGG 0.670626 1.2:+84026726 MS.gene037335:CDS
GGGTGGCATGCAAATGCCGA+TGG 0.671435 1.2:+84024779 MS.gene037335:CDS
GCTGGGAACCACTAGTCATG+TGG 0.677762 1.2:+84025170 MS.gene037335:CDS
ATGAGTCTACGATTCGTGAG+TGG 0.678627 1.2:+84024747 MS.gene037335:CDS
TCAATGTCAAAGAAATGACA+GGG 0.687101 1.2:+84023701 MS.gene037335:CDS
TTTACTCAAAATCTCGGACG+CGG 0.689301 1.2:+84026943 MS.gene037335:CDS
ATGAACTCAGCATGATGACG+AGG 0.692646 1.2:-84026799 None:intergenic
ATTGTGTAAAGCCTGACATG+GGG 0.693819 1.2:+84026896 MS.gene037335:CDS
CTGCATCAGACACATCACAA+TGG 0.698372 1.2:-84026984 None:intergenic
GGTCGCAATTATCTACATGG+TGG 0.698999 1.2:+84024680 MS.gene037335:CDS
GGCTGAAACCAACAACGAGG+AGG 0.705772 1.2:+84024183 MS.gene037335:CDS
TGGTGACCTGATGGTTGGAG+AGG 0.707195 1.2:+84027649 MS.gene037335:CDS
ATGATATACACAATAACACA+AGG 0.707929 1.2:+84024993 MS.gene037335:CDS
AAATGGTCTGATCATGAAGG+GGG 0.713662 1.2:-84028233 None:intergenic
AGGGCTGTAGATCTACACCA+TGG 0.722496 1.2:+84025064 MS.gene037335:CDS
GATAAATCACAGATCCGCTG+CGG 0.750646 1.2:+84029897 MS.gene037335:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 1.2:+84025102 MS.gene037335:CDS
GTGAGTGGTCAAGATCTGGG+TGG 0.773278 1.2:+84024762 MS.gene037335:CDS
TAAGGCTGAAACCAACAACG+AGG 0.802053 1.2:+84024180 MS.gene037335:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.2 gene 84023672 84030584 84023672 ID=MS.gene037335
chr1.2 mRNA 84023672 84030584 84023672 ID=MS.gene037335.t1;Parent=MS.gene037335
chr1.2 exon 84023672 84023787 84023672 ID=MS.gene037335.t1.exon1;Parent=MS.gene037335.t1
chr1.2 CDS 84023672 84023787 84023672 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84023983 84024082 84023983 ID=MS.gene037335.t1.exon2;Parent=MS.gene037335.t1
chr1.2 CDS 84023983 84024082 84023983 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84024169 84024307 84024169 ID=MS.gene037335.t1.exon3;Parent=MS.gene037335.t1
chr1.2 CDS 84024169 84024307 84024169 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84024388 84024451 84024388 ID=MS.gene037335.t1.exon4;Parent=MS.gene037335.t1
chr1.2 CDS 84024388 84024451 84024388 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84024535 84025273 84024535 ID=MS.gene037335.t1.exon5;Parent=MS.gene037335.t1
chr1.2 CDS 84024535 84025273 84024535 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84026676 84027005 84026676 ID=MS.gene037335.t1.exon6;Parent=MS.gene037335.t1
chr1.2 CDS 84026676 84027005 84026676 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84027491 84027790 84027491 ID=MS.gene037335.t1.exon7;Parent=MS.gene037335.t1
chr1.2 CDS 84027491 84027790 84027491 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84028225 84028297 84028225 ID=MS.gene037335.t1.exon8;Parent=MS.gene037335.t1
chr1.2 CDS 84028225 84028297 84028225 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84028397 84028482 84028397 ID=MS.gene037335.t1.exon9;Parent=MS.gene037335.t1
chr1.2 CDS 84028397 84028482 84028397 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84029811 84029930 84029811 ID=MS.gene037335.t1.exon10;Parent=MS.gene037335.t1
chr1.2 CDS 84029811 84029930 84029811 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
chr1.2 exon 84030526 84030584 84030526 ID=MS.gene037335.t1.exon11;Parent=MS.gene037335.t1
chr1.2 CDS 84030526 84030584 84030526 ID=cds.MS.gene037335.t1;Parent=MS.gene037335.t1
Gene Sequence

>MS.gene037335

TGTTTTGAAGGGGTTTCCTCAATGTGCCATCAATGTCAAAGAAATGACAGGGCCGGCGTTGTTAGATGCACAAAATGCAAACGGAAAAGATTTTGCACATCATGTATACAAAAGTGGTACAACTTCCTTAACTTCTGTTTTACTTTGTTTAACTTGCTTCATCCTATGTTGTGTCAGTTTGTTCTATTGTTTTTGAATATGCCTTGTTTGATAATTGGGATGTAGACCGAAGTTATTGCACATATTACATGTTTGGATTTTGGTTGATGAAATATTGATCAAACTATGCTTTTTTGTTGCCTTTTGCTAAGGTATCCTAAATTGAAAGAGAGTGATATTGCCAAGGCATGCCCTGTGTGCTGTGATAATTGCAATTGCAAAGCATGCTTGCGGTCTTTTAAACTCATCAATGTCAGTGATCCACCCTTCCACGATTAACTATTCTTCTGATTTTTCCGTAGTCTATATTTGAAATCTATCCATTTGGTTTACTATAGGAAATGAAGCGTAAGGCTGAAACCAACAACGAGGAGGAGGTTGAGTTCTCTAAGTATCTGCTGAAAGGACTTCTTCCACATTTAAGACGGTTGGATGAAGAACAAATGATTGAGAAGGAAAGAGAAGCCAGGAGACGAGGTATTCCTATATAGTCAGAGAAAATTTGTAGTTTCATATATTTTTCATTTTATAATTTGATTTTGGGATGTGAATTACAGGGCTCTCACTCTCAAAGCTAAATATAAAACCGGCGGATTATTCTAAAGATGAGCGTGTGTTCTGGTAAGGACTGATTTGGTATATTAATCAATGCTTTTTTTACTTATTCTAATGCTATATAGTTGTTGACTTCACACTTTTTGTAGTGACAACTGTAAAACATCAATATTTGACTACCACAGAAGCTGTACAAAATGCTCTTTTGACCTTTGTCTCCTCTGTTGCTGCGAGCTCCGTGGTGGGAAGCTTCTAGGTGGTGCAGATCCAATTAAGCTTGAGTATGATTTTCGAGGTCGCAATTATCTACATGGTGGAAATGAAGAAAAACATGTTAAAGAAAGTGTATCACATGCAGAGGATGAGTCTACGATTCGTGAGTGGTCAAGATCTGGGTGGCATGCAAATGCCGATGGTAGCATTCCTTGTCCAAAAGCTGATAATGAATGTGATCATGGTTTCCTTGAACTGAGAAGGATACTTCCTCCAAATTGCATCTCTGAGTTAGTTTGTAAAGCAAACAAACTTGCAGAAACAATCAAGCTTCAAGATGTAGAGGAGACTCGTGATAATAGGTGTTCTTGCTCGAAACCTGTTAGGTACACCGATGATATACACAATAACACAAGGAAGGCAGCTTTTCATGAAGATTCAAGTGACAAATTTTTATACTGTCCAAGGGCTGTAGATCTACACCATGGAGATTTAAAACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGCACATCTGGTTTAAGCTGGGAACCACTAGTCATGTGGCGTGCATTCCGTCAAATAACCAACTCCAAATACGATGTAGTTTTGGTTGTGAAGGCAGTTAATTGTTTAGATTGGTGTGAGGTTTGTTTGATTTCTCAATCCTTATGATGGAGGGGTTCTTACAGGAATTCATGTTTATTATCCATGTGTGAAACTTGTTTCTAATTTATTTAACCGACATTCTTAAAATTTGGATTGCACCTAACTTAACTCTACAAAACCGCCTTGTGAGGGGAGATGTATATCTCATTTATAAACATAGTTTGAGGCTATATCTCATCCAATGTGGAACTCTTATCAAACATATTTATTTTTTTAACTGGAAAGACTTTGCTTGAAAGTGACATCTAGTAGTGCTTGAACTGTAGCAGTTTGTTATTGTACTGTTGAAATACAACATTAGGGTTATGACTTGTGAGCCTCAAGCCAATTTTTTGAGTGCAATTGGCATGTGTTAGTCATAAAGTCGCAGTCGTTCGTGCTTCTCATATGTTGTTTCTCCCAACATTTTCAACAAACGCTGCTTCCTACTTCTCATTCAATATCTTGTAATCTTTTTAAATTTATCTAAATTTCTTGCTATCTGGTGTCCCAATTTGTTTTTGCATCACATATCCAGATTAATTGTTGTCATTGCATCCTCTTCATTCAATTTATCTATTGGTATTTTTGACTGTTATTATGTATTAGGGATGGCAATGGGTACCCTATGGGTAGGGTACTACAGTGCCCTTCCCCATACCCGTGTTTGTAAAAAATACTCGTGCCCAAGCCCGTATCCTCGTGGGCAACAACTTTAGTGCCCTTTCTCATAACCTATAGGTACCTTAGTGCTCATACCCGCATCCATTACCCGCACTTTAACTATGAAAAGACAATAAAAATATCACAATTGAAATAAAATTATGGTTTAAAATTTTTCAAATCAACTCATAAAATAGTACGAATCGTAGTTTTTAACAAAATAAAAAACATCCAAAACATCCTTCAAAAATTGTATACCAACAGAGGGGATTGGTTATTGTATTAGGTAGTTTTTAGGTTTAGACTTAGACTTAAATAGCTAAATAAATTAATTAATTATACACCAAAAATAAATAAATTATTTATCATATTATATAAATATGTATATGCTGGTTGCGGGGCTGGGTAGTATAGTACCCTTACCCATTTAAATTTGCAGGTAATACCCGGACCCAAACCCGAAGCCATGAAAGCAGGGTTTTACCCTACCCATTGTGGGTATTTTTAGCGGGTATCCATCGGGCCTGGTTCCAATTGCCATCCCTGTTACGTATACTTACTGCATGTTGATGTTAAGTTGGACTTATTGTTTTTTATGTTTCCTTGTTGAATTTTCTTTGTCCAGTTGTACTGCCTAACAGTCTCGGGTGAATTGTAACTGCTTTATGTTACTGCCTTTACTTGATATTCTGTTGCTGGTAATTTCTGCATTTTTATTTGAGTTGTTCATTGAATGCAGGGAGATATTAATATCCACCAATTCTTCACTGGGTACACAAATGGTCGCAGCGATTGGCTTAAATGGCCTGAGGTGTTGAAATTGAAAGATTGGCCTCCTTCTGATTTATTTCAGGAACTTTTACCTCGTCATCATGCTGAGTTCATATCTTCCTTGCCTTATAAAGAATACACAAATCCTTTTAGTGGTTCTCTCAACCTTGCTGTGAAGTTGCCGGATTATTGTGTAAAGCCTGACATGGGGCCAAGAACATATATTGCTTATGGATTTACTCAAAATCTCGGACGCGGTGATTCTGTGACTAAGCTCCATTGTGATGTGTCTGATGCAGTATGCTTCAAAACCTTTTTATATTTCTGGCATGTTTTTTCAGAATCTCTTAATTATTTGTGTTTTTTTTACGAAAGGTTTTATTGTTAATATGAATCGAGTATAGTATAAAGAAGTGCATAATTTGTAGGACATGTGCATTTTTGTTTTGTAAGCCATTTCATTTATCTGTTTATTTTCTTACGATCTGATTGTTGTGCAGCTTCAAGTATATGTCATAATTCTTTGTTCATTGCACTACCATAGATAATTTCTTCATTTGATTTGTTATTTCTCTTGGTATTTTTGTAATAATGACTCAAAAAAGTGTTCTTTTTATGACTAGTGGCATAAACTGCAAAGTATTTTTAGGACTTGAATAATGAATGATGCATTTCCTGATCAATTCTTTGTTAGCACTTTATAACTATTGATTTTTTTCTCCAAGTGAATATTTTATTATGAGGAAAATTAAATTATTATTTTTTTTGTAATTTTGCTATAAGGTAAATGTGTTGACTCATATCGCTAAAGTGGAACTAAAACCTGAGGAAATTAGTGTCATTAAGAAATTGACACGTAAGCACCTTGAGCAAGATAAGAGGGAGCTACATGGTGATGGGGAGGCGGTTGATATGTTTCACCAACTCTCTGACACTAATGATGGTGACCTGATGGTTGGAGAGGATCCCTTAGAAGGTGCTCTCTGGGATATTTTTCGGAGAGAAGATGTACCCAAATTGAAAGAATATCTAGAGAAGCATTTCAGAGAGTTCAGACATGTCAATTGCATTCCTTTAAAGCAGGTAGAATCGGCGTAGACAATTTTCCATTATCTAGCTCTATGGATGCCCCTCGTGACCATTTACTTTCTCTGCTGCTAACATGATATATAAATATCCCTGTTTTTTGTAACTTTTGGAGGGTTGCACTAGGATAAAATTGACACTATTACTTTCTTCCGAGTTAAGATTCAAATACACATGCCACAAAGGATTGTGTTGAAACATAATATAAAGTCTGACTGGTTTATTAATTTACGGTCCTTTCATTACTATGATTACAGTAGATACTTTTGTTCAGTGACCAGAGAGAAAGGAAATGCATTTTTGATGTAGATACCAGTCCAAAAGGTCATTGGTTGAATCAACATGTTTAATATGTTCCGTATCAAATTTGAAGTTTACAAGGCAGTCCTTGCTGTTTATTTTCTTGAATGAGATATCCTTTGGTAATGTAGGTTATTGACCCCCTTCATGATCAGACCATTTATCTGAATATGGAGCATAAGATGAAACTTAAGAAAGAGTATGGTTAGTAATATTTATAAATGTTCCTTTGTTGTTTGTAGATGGACTAGTTTGATCATTTAAATGGCACCACTTTGTTGACATTTCAACATTGACTTGTAGGAATTGAGCCTTGGACTTTTATTCAGAAGTTGGGAGATGCCGTTTTCATTCCTGCTGGTTTGCCTCACCAAGTCAGAAATCTGAAGGTAAAATTTATCGACAAAACCTACAGTATCATAGATACTGTTTTTGCTGTTCCCAATTAAAACAAGATTACAAGACACGTGTACTATTTTAACACCCAATTTTAACGTCATCAGCTTTCGTTAGTTTAGTCACACAGGATAATGGTACCGCCTTTCTGGACAGAAATAAGGGATGCCATTGCAAGGCTTGCTCTCACCGTGGATGTCACATAAAGCACTCATTGATTGTATAATTCTTCCTATGAGCTCCAATCTTTCTTTTAAGTGACTCTTGTGTTCTTTCTCATCACCACTCAAATCAAAGCTACTAAACCTATCTCCAACCCCACCATACTCAATAGCATACGACCTTGAAGCTGTGGTTTACTAACTCCAATTGTAATACAAATTTTAGTCAGTGCATTCTTTGTTATTCACCGGTTTTGTGTTACTTCCCAGAAGGAACTGCTCGTATTTAACGGGAAATCCGGTTGTGATTATTGGTGGCGGTGCTACTTGAATTGGAACGTAAGCCATCAGAAATTGGTGAATTACAAACGGTTTCCAACCAATTCCTCAGACAATGAACCGACGAATTGCGTCACCGGAACACTCAGCCATGTCGTGATTCATGAACCACTCTGGACCCTATTCGCCGCTGACATCACAATAAACCTCCGACAAGAACTTATTTTCAACAGTTTCAGAACCGACGACCATGGCGCTAACGTCAAATCATCAGCCATGATTGTAAGACTAGTAGTAAATCTCAAACATTATGAGAAAAGGGAAGAGAATCGACACAGATAGAGGGAGCGAGAAACAGAGAGAAAACCAAAAACGGAAATCAAACGGTGTTAAAATGTGGTGATTAAATGGTACGTGTGTCTTGTTTTAATTGGGAACAGCAAAAACAGTACCTATATGGTACTGTAGGCAAGTTTTGTCCAAAATTTATTTACATGGTACAAATTTCTTTGTTTGCCAAGAAGTGTGTAACGTCTATAAAATTTTGCTTGGAGCGAGGAATAACAGCTTGAATTAGATTAAGATCTGAATTGAGATATGATTTATTCTGTGTTTTATCTCTTATCCTTGGTGCTTGTATTCTCTAAAGTTTAGTAGATTGTTTAGTATTTTTTATGGCCAACTTTTTGATATATTTTGTAACTTTACAAGGCTAAAATTGAATTTGGATATTTCCTAATTAGTACAGCGTGATGGCTTCAACCTATGCAAATTGTTGAAGCCATACATCACAAACTCACTTTTTATGTCACTGTTGCTTTTTTGTTGAAACAATGCTTGAAATGTTTGTTTTATGTAATATATGCATAATTCTGTTGCAGTCCTGCATCAAGGTTGCACTTGATTTTGTCTCTCCCGAACATATTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAGCTTCCGATAAATCACAGATCCGCTGCGGACAAGTTTGAGGTTTGCCAGACTACTTTCATTGATTTGATTTTACTTTTCCTATATATAAGGATACAAGACTCTTAATCCCTATTGAATAGGTAGTAAATTCATGAAAATAACTAAGTAATATGGAAAGTAATCTCAAGAGAAATTCCAACCAAGAAACTAATCCTATGAGATAAACTAAAATATTCCAACATAATATTAGAATATTATAAAATTCTTTTTATATATCCTCGCTCACCGGAGGGCAAGTGTGCTTGCATAACAGTATTGCTGACAAGGTTCTAACTTCTAATATTGACTGTTTTAATTCAGATAAAAGCCTTGTTGAGTGTATGAAATCTTGATCATTAACCAATTTCAGTTCTATGTTCCCGTGTTATGTCATCATGTCAACAATTTCTTATTGGGATTTTATGTACTGTTTTTTTCCTGGTGTCTGCTTCTAATATTGACTGTTTTAATTCAGATAAAATCCTTGTTGAATGTATGAAATCTTGGTCATCAAGCCTATTTCAGTTCTATGTTCCCGTGTTCTGTCATCATGTCAACAATTTCTTATTGGGATTTTATGTACTGTTTTTTACTGATGACTGCTTCTTGGTACCAGGTGAAGAAAATTGCCGTACATGCTATGCTTGATGTGGTTGAAAAATTAGAGAAAGCAAG
Protein sequence

>MS.gene037335.t1

CFEGVSSMCHQCQRNDRAGVVRCTKCKRKRFCTSCIQKWYPKLKESDIAKACPVCCDNCNCKACLRSFKLINEMKRKAETNNEEEVEFSKYLLKGLLPHLRRLDEEQMIEKEREARRRGLSLSKLNIKPADYSKDERVFCDNCKTSIFDYHRSCTKCSFDLCLLCCCELRGGKLLGGADPIKLEYDFRGRNYLHGGNEEKHVKESVSHAEDESTIREWSRSGWHANADGSIPCPKADNECDHGFLELRRILPPNCISELVCKANKLAETIKLQDVEETRDNRCSCSKPVRYTDDIHNNTRKAAFHEDSSDKFLYCPRAVDLHHGDLKHFQWHWSKGEPVIVSNVLECTSGLSWEPLVMWRAFRQITNSKYDVVLVVKAVNCLDWCEGDINIHQFFTGYTNGRSDWLKWPEVLKLKDWPPSDLFQELLPRHHAEFISSLPYKEYTNPFSGSLNLAVKLPDYCVKPDMGPRTYIAYGFTQNLGRGDSVTKLHCDVSDAVNVLTHIAKVELKPEEISVIKKLTRKHLEQDKRELHGDGEAVDMFHQLSDTNDGDLMVGEDPLEGALWDIFRREDVPKLKEYLEKHFREFRHVNCIPLKQVIDPLHDQTIYLNMEHKMKLKKEYGIEPWTFIQKLGDAVFIPAGLPHQVRNLKSCIKVALDFVSPEHIGECFRLTEEFRKLPINHRSAADKFEVKKIAVHAMLDVVEKLEKA