Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037434.t1 | GAU21539.1 | 46.7 | 345 | 82 | 7 | 12 | 260 | 8 | 346 | 5.10E-61 | 244.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037434.t1 | A0A2Z6MRF5 | 46.7 | 345 | 82 | 7 | 12 | 260 | 8 | 346 | 3.7e-61 | 244.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene037434.t1 | TR | ARID |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037434.t1 | MTR_5g095070 | 50.000 | 256 | 66 | 7 | 6 | 210 | 9 | 253 | 3.91e-65 | 204 |
| MS.gene037434.t1 | MTR_1g084150 | 56.250 | 160 | 35 | 2 | 135 | 260 | 64 | 222 | 9.59e-54 | 174 |
| MS.gene037434.t1 | MTR_4g015240 | 85.507 | 69 | 10 | 0 | 192 | 260 | 32 | 100 | 1.35e-35 | 123 |
| MS.gene037434.t1 | MTR_5g080030 | 62.105 | 95 | 36 | 0 | 30 | 124 | 40 | 134 | 4.62e-35 | 130 |
| MS.gene037434.t1 | MTR_5g080030 | 59.770 | 87 | 32 | 1 | 130 | 213 | 195 | 281 | 1.16e-25 | 104 |
| MS.gene037434.t1 | MTR_5g080030 | 51.515 | 132 | 50 | 6 | 130 | 254 | 271 | 395 | 2.22e-25 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTAAGTATCGAAATAAAC+TGG | 0.303192 | 4.1:-81006452 | None:intergenic |
| GTAAAGCAGATACAAGTCTA+AGG | 0.319113 | 4.1:-81005453 | None:intergenic |
| AACGTTACCATAGGTGAGTT+TGG | 0.366922 | 4.1:-81005089 | None:intergenic |
| TTTGAGAAATTGTACTTCTC+TGG | 0.372455 | 4.1:+81005788 | MS.gene037434:CDS |
| ACAAAAGAGTAATTTGTTAG+AGG | 0.388722 | 4.1:-81006365 | None:intergenic |
| CAGAAAATGCCTCGTCTGTC+CGG | 0.397968 | 4.1:+81006317 | MS.gene037434:CDS |
| GATGTCTGTCGACGCTAAAC+AGG | 0.415004 | 4.1:+81006115 | MS.gene037434:CDS |
| AGGTTGCTCTACAAAACAAA+TGG | 0.427597 | 4.1:+81005675 | MS.gene037434:intron |
| AAACGGCGAGGTGGTCCAAC+TGG | 0.434685 | 4.1:+81005981 | MS.gene037434:CDS |
| GTCTCTGCACTGAAACTAGA+AGG | 0.436460 | 4.1:+81005707 | MS.gene037434:CDS |
| ATCGAAATAAACTGGGAAGA+AGG | 0.456327 | 4.1:-81006444 | None:intergenic |
| AGACTTGTATCTGCTTTACT+TGG | 0.470775 | 4.1:+81005457 | MS.gene037434:CDS |
| ACTCATGATCCAGACATAGA+TGG | 0.475139 | 4.1:+81005945 | MS.gene037434:intron |
| TCGATTAAAAGCTCATGTTC+CGG | 0.480755 | 4.1:+81006043 | MS.gene037434:CDS |
| AGAAGAGTATTGTTTCCCCT+TGG | 0.498879 | 4.1:-81005116 | None:intergenic |
| TACGCATGGCTATTGATGCT+TGG | 0.507330 | 4.1:+81006087 | MS.gene037434:CDS |
| CTATGGTAACGTTGTGTCCA+AGG | 0.513836 | 4.1:+81005099 | MS.gene037434:CDS |
| ACTCAAGTGTTGGATTCCAC+TGG | 0.519362 | 4.1:+81005163 | MS.gene037434:CDS |
| TCGAAATAAACTGGGAAGAA+GGG | 0.522528 | 4.1:-81006443 | None:intergenic |
| ACAAGTCTAAGGGTGTTTCT+CGG | 0.530221 | 4.1:-81005442 | None:intergenic |
| TGTATAGAAGTATTGCAAAG+AGG | 0.532624 | 4.1:-81005763 | None:intergenic |
| CATTTCAAAAGCCACGTTCG+CGG | 0.533360 | 4.1:-81006399 | None:intergenic |
| TACTTGGAAGTCACGAGAAG+AGG | 0.535118 | 4.1:+81005473 | MS.gene037434:CDS |
| TGTCAAAGTCACTCAAGTGT+TGG | 0.544849 | 4.1:+81005153 | MS.gene037434:CDS |
| GCTCATGTTCCGGACATAGT+TGG | 0.547344 | 4.1:+81006053 | MS.gene037434:CDS |
| TCAGATGCCAAACTCACCTA+TGG | 0.549356 | 4.1:+81005082 | MS.gene037434:CDS |
| ATGGCAAAGAAAAGAAGAAA+CGG | 0.560257 | 4.1:+81005964 | MS.gene037434:CDS |
| GGTTGCTCTACAAAACAAAT+GGG | 0.562695 | 4.1:+81005676 | MS.gene037434:intron |
| TATGGTAACGTTGTGTCCAA+GGG | 0.564205 | 4.1:+81005100 | MS.gene037434:CDS |
| ATATCAAATATTTCTCAAGC+AGG | 0.571118 | 4.1:+81006013 | MS.gene037434:CDS |
| TGGTCTCTTAGATACCTTTG+GGG | 0.580404 | 4.1:-81005814 | None:intergenic |
| GGTCTCTTAGATACCTTTGG+GGG | 0.580925 | 4.1:-81005813 | None:intergenic |
| CTTGGACACAACGTTACCAT+AGG | 0.590341 | 4.1:-81005098 | None:intergenic |
| TAAAGCAGATACAAGTCTAA+GGG | 0.593030 | 4.1:-81005452 | None:intergenic |
| TTGGAAGTCACGAGAAGAGG+AGG | 0.597845 | 4.1:+81005476 | MS.gene037434:CDS |
| TGTTACGCAGTTAGAGTCAC+CGG | 0.618224 | 4.1:-81006336 | None:intergenic |
| TTTCATGTAGCCATATGTGG+AGG | 0.619171 | 4.1:+81006238 | MS.gene037434:intron |
| CAACAACGCAGCCGCGAACG+TGG | 0.625266 | 4.1:+81006388 | MS.gene037434:CDS |
| AAGCATCAATAGCCATGCGT+AGG | 0.630888 | 4.1:-81006085 | None:intergenic |
| AACAACACAGAAACAATGGA+AGG | 0.643932 | 4.1:+81005028 | None:intergenic |
| AATGGAAGGCAACAACACTA+CGG | 0.644784 | 4.1:+81005042 | None:intergenic |
| AAAGATGAAGGAAACAAACA+AGG | 0.649227 | 4.1:+81006268 | MS.gene037434:CDS |
| AGTAAGTATCGAAATAAACT+GGG | 0.654348 | 4.1:-81006451 | None:intergenic |
| TGACATGAGATTGAAGCCAG+TGG | 0.658000 | 4.1:-81005179 | None:intergenic |
| GAAAAGAAGAAACGGCGAGG+TGG | 0.658094 | 4.1:+81005972 | MS.gene037434:CDS |
| TTAGAGTCACCGGACAGACG+AGG | 0.678770 | 4.1:-81006326 | None:intergenic |
| ATGGTAACGTTGTGTCCAAG+GGG | 0.685604 | 4.1:+81005101 | MS.gene037434:CDS |
| AAAGAAAAGAAGAAACGGCG+AGG | 0.733773 | 4.1:+81005969 | MS.gene037434:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATATATCATCTTTATT+TGG | + | chr4.1:81005354-81005373 | MS.gene037434:intron | 10.0% |
| !! | AATTTATCTTGAATGTTTCA+AGG | + | chr4.1:81005655-81005674 | MS.gene037434:intron | 20.0% |
| !! | CATGAAAAAGTAATTAGAAA+TGG | - | chr4.1:81006225-81006244 | None:intergenic | 20.0% |
| !! | TACAACAAATTAATAATACC+TGG | - | chr4.1:81005508-81005527 | None:intergenic | 20.0% |
| !!! | AATGCATGTGTATATATTTT+AGG | + | chr4.1:81005552-81005571 | MS.gene037434:intron | 20.0% |
| ! | ATATCAAATATTTCTCAAGC+AGG | + | chr4.1:81006013-81006032 | MS.gene037434:CDS | 25.0% |
| ! | CACACAAATTTAATCAAGAA+AGG | + | chr4.1:81005604-81005623 | MS.gene037434:intron | 25.0% |
| !!! | ACAAAAGAGTAATTTGTTAG+AGG | - | chr4.1:81006368-81006387 | None:intergenic | 25.0% |
| !!! | AGTAAGTATCGAAATAAACT+GGG | - | chr4.1:81006454-81006473 | None:intergenic | 25.0% |
| AAAGATGAAGGAAACAAACA+AGG | + | chr4.1:81006268-81006287 | MS.gene037434:CDS | 30.0% | |
| ATGCTTGATATGAAGATGTT+TGG | + | chr4.1:81005891-81005910 | MS.gene037434:intron | 30.0% | |
| ATGGCAAAGAAAAGAAGAAA+CGG | + | chr4.1:81005964-81005983 | MS.gene037434:CDS | 30.0% | |
| ATTTCAAACAAACCAAGTAG+AGG | - | chr4.1:81006199-81006218 | None:intergenic | 30.0% | |
| GCATATAAGTAACCACAAAT+TGG | - | chr4.1:81005837-81005856 | None:intergenic | 30.0% | |
| TAAAGCAGATACAAGTCTAA+GGG | - | chr4.1:81005455-81005474 | None:intergenic | 30.0% | |
| TTTGAGAAATTGTACTTCTC+TGG | + | chr4.1:81005788-81005807 | MS.gene037434:CDS | 30.0% | |
| TTTGCATGCATTCAAAATCA+AGG | + | chr4.1:81005241-81005260 | MS.gene037434:intron | 30.0% | |
| ! | ACATTTTTACCAACTATGTC+CGG | - | chr4.1:81006065-81006084 | None:intergenic | 30.0% |
| !! | CAGTAAGTATCGAAATAAAC+TGG | - | chr4.1:81006455-81006474 | None:intergenic | 30.0% |
| !! | TGTATAGAAGTATTGCAAAG+AGG | - | chr4.1:81005766-81005785 | None:intergenic | 30.0% |
| AGACTTGTATCTGCTTTACT+TGG | + | chr4.1:81005457-81005476 | MS.gene037434:CDS | 35.0% | |
| AGGTTGCTCTACAAAACAAA+TGG | + | chr4.1:81005675-81005694 | MS.gene037434:intron | 35.0% | |
| ATCGAAATAAACTGGGAAGA+AGG | - | chr4.1:81006447-81006466 | None:intergenic | 35.0% | |
| CTTGTTGAGTTTAACATGCA+GGG | + | chr4.1:81005304-81005323 | MS.gene037434:intron | 35.0% | |
| GGTTGCTCTACAAAACAAAT+GGG | + | chr4.1:81005676-81005695 | MS.gene037434:intron | 35.0% | |
| GTAAAGCAGATACAAGTCTA+AGG | - | chr4.1:81005456-81005475 | None:intergenic | 35.0% | |
| GTATCTAAGAGACCAATTTG+TGG | + | chr4.1:81005822-81005841 | MS.gene037434:intron | 35.0% | |
| TCGAAATAAACTGGGAAGAA+GGG | - | chr4.1:81006446-81006465 | None:intergenic | 35.0% | |
| TCGATTAAAAGCTCATGTTC+CGG | + | chr4.1:81006043-81006062 | MS.gene037434:CDS | 35.0% | |
| TCTTGTTGAGTTTAACATGC+AGG | + | chr4.1:81005303-81005322 | MS.gene037434:intron | 35.0% | |
| TTGGTCTCTTAGATACCTTT+GGG | - | chr4.1:81005818-81005837 | None:intergenic | 35.0% | |
| ! | ATTGGTCTCTTAGATACCTT+TGG | - | chr4.1:81005819-81005838 | None:intergenic | 35.0% |
| ! | TTTTCTTTGCCATCTATGTC+TGG | - | chr4.1:81005957-81005976 | None:intergenic | 35.0% |
| ! | TTTTTGCTTTCCTCCACATA+TGG | - | chr4.1:81006251-81006270 | None:intergenic | 35.0% |
| !! | CTTTTTCATGTAGCCATATG+TGG | + | chr4.1:81006235-81006254 | MS.gene037434:intron | 35.0% |
| !! | TTTTGCAATTAGCCTCTACT+TGG | + | chr4.1:81006184-81006203 | MS.gene037434:intron | 35.0% |
| !!! | TGATATCCACTTTTTCCAGT+TGG | - | chr4.1:81005999-81006018 | None:intergenic | 35.0% |
| AAAGAAAAGAAGAAACGGCG+AGG | + | chr4.1:81005969-81005988 | MS.gene037434:CDS | 40.0% | |
| AACGTTACCATAGGTGAGTT+TGG | - | chr4.1:81005092-81005111 | None:intergenic | 40.0% | |
| ACTCATGATCCAGACATAGA+TGG | + | chr4.1:81005945-81005964 | MS.gene037434:intron | 40.0% | |
| GGAGGAAAGCAAAAAGATGA+AGG | + | chr4.1:81006256-81006275 | MS.gene037434:CDS | 40.0% | |
| TGGTAAAAATGTCCTACGCA+TGG | + | chr4.1:81006073-81006092 | MS.gene037434:CDS | 40.0% | |
| TGGTCTCTTAGATACCTTTG+GGG | - | chr4.1:81005817-81005836 | None:intergenic | 40.0% | |
| TGTCAAAGTCACTCAAGTGT+TGG | + | chr4.1:81005153-81005172 | MS.gene037434:CDS | 40.0% | |
| TTTCATGTAGCCATATGTGG+AGG | + | chr4.1:81006238-81006257 | MS.gene037434:intron | 40.0% | |
| ! | TATGGTAACGTTGTGTCCAA+GGG | + | chr4.1:81005100-81005119 | MS.gene037434:CDS | 40.0% |
| !! | ACAAGTCTAAGGGTGTTTCT+CGG | - | chr4.1:81005445-81005464 | None:intergenic | 40.0% |
| !! | AGAAGAGTATTGTTTCCCCT+TGG | - | chr4.1:81005119-81005138 | None:intergenic | 40.0% |
| AAGCATCAATAGCCATGCGT+AGG | - | chr4.1:81006088-81006107 | None:intergenic | 45.0% | |
| CATTTCAAAAGCCACGTTCG+CGG | - | chr4.1:81006402-81006421 | None:intergenic | 45.0% | |
| CTTGGACACAACGTTACCAT+AGG | - | chr4.1:81005101-81005120 | None:intergenic | 45.0% | |
| GGTCTCTTAGATACCTTTGG+GGG | - | chr4.1:81005816-81005835 | None:intergenic | 45.0% | |
| GTCTCTGCACTGAAACTAGA+AGG | + | chr4.1:81005707-81005726 | MS.gene037434:CDS | 45.0% | |
| TACGCATGGCTATTGATGCT+TGG | + | chr4.1:81006087-81006106 | MS.gene037434:CDS | 45.0% | |
| TACTTGGAAGTCACGAGAAG+AGG | + | chr4.1:81005473-81005492 | MS.gene037434:CDS | 45.0% | |
| TCAGATGCCAAACTCACCTA+TGG | + | chr4.1:81005082-81005101 | MS.gene037434:CDS | 45.0% | |
| TGACATGAGATTGAAGCCAG+TGG | - | chr4.1:81005182-81005201 | None:intergenic | 45.0% | |
| TGTTACGCAGTTAGAGTCAC+CGG | - | chr4.1:81006339-81006358 | None:intergenic | 45.0% | |
| ! | ATGGTAACGTTGTGTCCAAG+GGG | + | chr4.1:81005101-81005120 | MS.gene037434:CDS | 45.0% |
| ! | CTATGGTAACGTTGTGTCCA+AGG | + | chr4.1:81005099-81005118 | MS.gene037434:CDS | 45.0% |
| !! | ACTCAAGTGTTGGATTCCAC+TGG | + | chr4.1:81005163-81005182 | MS.gene037434:CDS | 45.0% |
| CAGAAAATGCCTCGTCTGTC+CGG | + | chr4.1:81006317-81006336 | MS.gene037434:CDS | 50.0% | |
| GAAAAGAAGAAACGGCGAGG+TGG | + | chr4.1:81005972-81005991 | MS.gene037434:CDS | 50.0% | |
| GATGTCTGTCGACGCTAAAC+AGG | + | chr4.1:81006115-81006134 | MS.gene037434:CDS | 50.0% | |
| GCTCATGTTCCGGACATAGT+TGG | + | chr4.1:81006053-81006072 | MS.gene037434:CDS | 50.0% | |
| GGTGGTCCAACTGGAAAAAG+TGG | + | chr4.1:81005990-81006009 | MS.gene037434:CDS | 50.0% | |
| TTGGAAGTCACGAGAAGAGG+AGG | + | chr4.1:81005476-81005495 | MS.gene037434:CDS | 50.0% | |
| ! | GAGAAGAGGAGGTTTTCACC+AGG | + | chr4.1:81005487-81005506 | MS.gene037434:CDS | 50.0% |
| TACTTCTCTGGCACCCCCAA+AGG | + | chr4.1:81005800-81005819 | MS.gene037434:CDS | 55.0% | |
| TTAGAGTCACCGGACAGACG+AGG | - | chr4.1:81006329-81006348 | None:intergenic | 55.0% | |
| AAACGGCGAGGTGGTCCAAC+TGG | + | chr4.1:81005981-81006000 | MS.gene037434:CDS | 60.0% | |
| CAACAACGCAGCCGCGAACG+TGG | + | chr4.1:81006388-81006407 | MS.gene037434:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 81005043 | 81006475 | 81005043 | ID=MS.gene037434 |
| chr4.1 | mRNA | 81005043 | 81006475 | 81005043 | ID=MS.gene037434.t1;Parent=MS.gene037434 |
| chr4.1 | exon | 81005043 | 81005197 | 81005043 | ID=MS.gene037434.t1.exon1;Parent=MS.gene037434.t1 |
| chr4.1 | CDS | 81005043 | 81005197 | 81005043 | ID=cds.MS.gene037434.t1;Parent=MS.gene037434.t1 |
| chr4.1 | exon | 81005433 | 81005508 | 81005433 | ID=MS.gene037434.t1.exon2;Parent=MS.gene037434.t1 |
| chr4.1 | CDS | 81005433 | 81005508 | 81005433 | ID=cds.MS.gene037434.t1;Parent=MS.gene037434.t1 |
| chr4.1 | exon | 81005677 | 81005821 | 81005677 | ID=MS.gene037434.t1.exon3;Parent=MS.gene037434.t1 |
| chr4.1 | CDS | 81005677 | 81005821 | 81005677 | ID=cds.MS.gene037434.t1;Parent=MS.gene037434.t1 |
| chr4.1 | exon | 81005958 | 81006136 | 81005958 | ID=MS.gene037434.t1.exon4;Parent=MS.gene037434.t1 |
| chr4.1 | CDS | 81005958 | 81006136 | 81005958 | ID=cds.MS.gene037434.t1;Parent=MS.gene037434.t1 |
| chr4.1 | exon | 81006248 | 81006475 | 81006248 | ID=MS.gene037434.t1.exon5;Parent=MS.gene037434.t1 |
| chr4.1 | CDS | 81006248 | 81006475 | 81006248 | ID=cds.MS.gene037434.t1;Parent=MS.gene037434.t1 |
| Gene Sequence |
| Protein sequence |
