AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037788

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037788.t1 MTR_4g021790 84.672 274 36 1 1 268 1 274 1.04e-171 475
MS.gene037788.t1 MTR_4g021855 74.419 301 44 2 1 268 1 301 1.02e-161 451
MS.gene037788.t1 MTR_4g021760 75.519 241 52 2 1 234 1 241 2.47e-126 362
MS.gene037788.t1 MTR_4g021845 78.341 217 41 1 1 211 1 217 3.47e-123 350
MS.gene037788.t1 MTR_5g055260 59.924 262 72 4 1 256 1 235 2.32e-94 278
MS.gene037788.t1 MTR_4g023980 78.102 137 30 0 49 185 35 171 3.97e-76 229
MS.gene037788.t1 MTR_1g032570 44.534 247 100 4 10 219 11 257 1.60e-62 207
MS.gene037788.t1 MTR_6g045327 43.802 242 122 5 1 230 1 240 5.29e-52 174
MS.gene037788.t1 MTR_8g105600 41.525 236 112 3 10 219 9 244 1.81e-50 174
MS.gene037788.t1 MTR_2g034960 39.167 240 116 3 10 219 29 268 1.23e-49 172
MS.gene037788.t1 MTR_4g121020 37.945 253 117 4 10 226 31 279 2.23e-49 172
MS.gene037788.t1 MTR_4g121020 37.945 253 117 4 10 226 31 279 2.37e-49 172
MS.gene037788.t1 MTR_3g086100 38.934 244 115 4 10 219 13 256 3.00e-49 171
MS.gene037788.t1 MTR_8g079940 38.683 243 117 4 9 219 24 266 4.81e-49 170
MS.gene037788.t1 MTR_1g013170 37.743 257 123 5 7 226 2 258 1.72e-44 157
MS.gene037788.t1 MTR_1g032570 42.553 188 78 2 62 219 1 188 2.42e-43 155
MS.gene037788.t1 MTR_4g131600 36.585 246 127 5 2 218 12 257 1.63e-42 153
MS.gene037788.t1 MTR_3g106220 36.694 248 119 4 10 219 8 255 3.41e-42 152
MS.gene037788.t1 MTR_7g117705 36.179 246 123 5 7 218 2 247 7.61e-42 149
MS.gene037788.t1 MTR_8g093040 41.553 219 105 6 10 221 14 216 9.43e-41 142
MS.gene037788.t1 MTR_1g013180 36.400 250 125 5 7 222 153 402 3.61e-40 147
MS.gene037788.t1 MTR_4g131570 34.263 251 129 6 10 225 17 266 6.43e-39 143
MS.gene037788.t1 MTR_6g016850 43.210 162 83 3 1 154 1 161 3.62e-38 132
MS.gene037788.t1 MTR_4g131580 34.008 247 132 5 10 225 17 263 3.97e-38 141
MS.gene037788.t1 MTR_2g450070 32.157 255 130 3 10 221 18 272 7.59e-38 140
MS.gene037788.t1 MTR_2g450070 32.157 255 130 3 10 221 18 272 9.85e-38 140
MS.gene037788.t1 MTR_3g086100 38.919 185 86 2 62 219 1 185 1.91e-36 135
MS.gene037788.t1 MTR_3g102590 36.449 214 103 5 10 215 8 196 5.83e-35 126
MS.gene037788.t1 MTR_7g026400 37.949 195 102 4 44 223 28 218 1.39e-33 128
MS.gene037788.t1 MTR_3g102600 34.615 234 110 5 10 236 8 205 4.63e-33 120
MS.gene037788.t1 MTR_3g102590 35.714 210 102 5 10 211 8 192 3.96e-32 117
MS.gene037788.t1 MTR_0450s0040 31.004 229 139 4 15 227 9 234 2.62e-30 116
MS.gene037788.t1 MTR_4g121020 37.647 170 81 1 82 226 6 175 9.15e-30 117
MS.gene037788.t1 MTR_3g102600 35.079 191 88 3 46 236 2 156 9.27e-30 110
MS.gene037788.t1 MTR_3g102600 33.175 211 99 4 10 213 8 183 1.37e-28 108
MS.gene037788.t1 MTR_3g102600 33.333 168 77 2 46 213 2 134 3.78e-25 97.4
MS.gene037788.t1 MTR_5g041350 51.429 70 33 1 162 230 15 84 7.07e-17 79.7
MS.gene037788.t1 MTR_1g013160 31.757 148 74 2 65 185 1 148 7.47e-17 76.3
MS.gene037788.t1 MTR_6g032990 63.636 55 19 1 162 215 42 96 2.61e-16 78.2
MS.gene037788.t1 MTR_2g086450 55.072 69 29 2 148 215 32 99 1.62e-15 75.9
MS.gene037788.t1 MTR_4g064730 50.725 69 34 0 151 219 120 188 1.41e-14 72.8
MS.gene037788.t1 MTR_7g098250 48.052 77 39 1 155 230 255 331 1.63e-14 73.2
MS.gene037788.t1 MTR_2g016220 50.000 72 31 2 144 214 75 142 3.17e-14 72.0
MS.gene037788.t1 MTR_2g016220 50.000 72 31 2 144 214 75 142 3.22e-14 72.0
MS.gene037788.t1 MTR_2g090675 42.553 94 51 2 127 218 125 217 3.59e-14 72.0
MS.gene037788.t1 MTR_7g089010 55.932 59 26 0 161 219 90 148 6.76e-14 70.5
MS.gene037788.t1 MTR_4g081710 56.364 55 23 1 162 215 17 71 9.16e-14 70.5
MS.gene037788.t1 MTR_7g088070 49.315 73 34 2 153 222 41 113 9.38e-14 69.7
MS.gene037788.t1 MTR_7g088070 44.318 88 46 2 153 237 41 128 9.49e-14 70.1
MS.gene037788.t1 MTR_7g115530 48.571 70 35 1 162 230 218 287 2.56e-13 68.9
MS.gene037788.t1 MTR_1g080330 54.545 66 28 2 151 215 165 229 2.82e-13 69.3
MS.gene037788.t1 MTR_1g080330 55.385 65 27 2 151 214 165 228 3.35e-13 68.6
MS.gene037788.t1 MTR_4g078810 55.172 58 26 0 161 218 157 214 3.37e-13 69.3
MS.gene037788.t1 MTR_7g115530 48.571 70 35 1 162 230 218 287 4.15e-13 68.6
MS.gene037788.t1 MTR_4g098870 40.000 105 54 2 161 265 329 424 4.69e-13 68.9
MS.gene037788.t1 MTR_4g098870 40.000 105 54 2 161 265 309 404 4.70e-13 68.9
MS.gene037788.t1 MTR_4g098870 40.000 105 54 2 161 265 329 424 4.85e-13 68.9
MS.gene037788.t1 MTR_7g115530 48.571 70 35 1 162 230 218 287 5.02e-13 68.9
MS.gene037788.t1 MTR_4g098870 40.000 105 54 2 161 265 309 404 5.54e-13 68.9
MS.gene037788.t1 MTR_4g098870 40.000 105 54 2 161 265 348 443 5.60e-13 68.9
MS.gene037788.t1 MTR_2g016220 49.275 69 30 2 144 211 75 139 8.59e-13 67.8
MS.gene037788.t1 MTR_0223s0040 50.000 68 32 2 148 214 5 71 1.01e-12 67.0
MS.gene037788.t1 MTR_7g115530 59.259 54 21 1 162 214 218 271 1.15e-12 67.4
MS.gene037788.t1 MTR_1g053835 50.847 59 28 1 158 215 254 312 1.40e-12 67.4
MS.gene037788.t1 MTR_3g450310 51.562 64 30 1 152 214 9 72 1.52e-12 65.5
MS.gene037788.t1 MTR_1g093080 46.250 80 35 2 143 221 199 271 1.63e-12 67.0
MS.gene037788.t1 MTR_7g069660 48.438 64 32 1 152 214 61 124 1.92e-12 66.6
MS.gene037788.t1 MTR_7g093030 52.632 57 26 1 159 214 209 265 2.32e-12 66.2
MS.gene037788.t1 MTR_5g014040 52.542 59 28 0 161 219 310 368 2.81e-12 66.6
MS.gene037788.t1 MTR_7g093030 52.632 57 26 1 159 214 209 265 3.02e-12 66.2
MS.gene037788.t1 MTR_4g086835 50.000 60 30 0 160 219 272 331 4.12e-12 65.9
MS.gene037788.t1 MTR_2g027860 58.182 55 22 1 162 215 49 103 5.55e-12 65.1
MS.gene037788.t1 MTR_2g084230 44.595 74 37 2 142 214 80 150 8.94e-12 64.7
MS.gene037788.t1 MTR_2g084230 44.595 74 37 2 142 214 80 150 1.08e-11 64.7
MS.gene037788.t1 MTR_8g086410 44.776 67 36 1 149 214 98 164 1.11e-11 64.7
MS.gene037788.t1 MTR_8g086410 44.776 67 36 1 149 214 96 162 1.17e-11 64.7
MS.gene037788.t1 MTR_1g112370 47.143 70 34 2 148 214 6 75 1.26e-11 64.3
MS.gene037788.t1 MTR_2g023580 53.571 56 25 1 160 214 53 108 1.43e-11 63.2
MS.gene037788.t1 MTR_6g444980 41.758 91 47 2 158 247 31 116 1.79e-11 63.9
MS.gene037788.t1 MTR_6g444980 54.237 59 26 1 158 215 31 89 1.84e-11 63.9
MS.gene037788.t1 MTR_4g113140 45.455 77 40 2 152 227 226 301 1.88e-11 63.9
MS.gene037788.t1 MTR_1g053800 54.098 61 27 1 158 217 173 233 3.14e-11 63.2
MS.gene037788.t1 MTR_1g053830 44.068 59 32 1 158 215 14 72 3.33e-11 58.9
MS.gene037788.t1 MTR_2g027800 40.217 92 50 2 158 244 35 126 3.78e-11 62.8
MS.gene037788.t1 MTR_1g090670 56.364 55 23 1 162 215 44 98 4.90e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037788.t1 AT2G01760 38.672 256 112 6 7 219 4 257 1.11e-50 171
MS.gene037788.t1 AT2G01760 33.660 306 128 7 7 239 4 307 2.03e-46 160
MS.gene037788.t1 AT1G67710 39.344 244 113 6 11 219 8 251 1.64e-44 157
MS.gene037788.t1 AT3G16857 36.264 273 114 7 10 226 33 301 4.60e-43 155
MS.gene037788.t1 AT3G16857 36.264 273 114 7 10 226 33 301 5.44e-43 155
MS.gene037788.t1 AT2G25180 34.507 284 150 7 10 262 13 291 7.16e-41 149
MS.gene037788.t1 AT4G31920 36.797 231 128 4 10 222 13 243 8.00e-41 148
MS.gene037788.t1 AT3G16857 36.957 230 99 5 43 226 2 231 9.18e-38 140
MS.gene037788.t1 AT2G01760 38.144 194 82 4 62 219 1 192 1.38e-35 129
MS.gene037788.t1 AT5G58080 35.391 243 119 7 10 219 14 251 2.92e-33 127
MS.gene037788.t1 AT1G67710 40.323 186 83 4 62 219 1 186 6.86e-33 125
MS.gene037788.t1 AT1G49190 32.258 248 131 8 10 220 30 277 1.27e-30 118
MS.gene037788.t1 AT3G62670 34.518 197 107 5 46 220 78 274 1.15e-27 110
MS.gene037788.t1 AT1G67710 39.759 166 72 4 82 219 12 177 1.57e-25 104
MS.gene037788.t1 AT5G49240 28.390 236 136 5 9 211 37 272 5.13e-23 95.9
MS.gene037788.t1 AT5G07210 28.358 268 130 8 16 223 18 283 1.25e-21 94.4
MS.gene037788.t1 AT5G07210 29.787 235 109 7 43 223 59 291 7.20e-21 92.4
MS.gene037788.t1 AT1G49190 32.800 125 77 2 10 127 30 154 1.37e-17 82.8
MS.gene037788.t1 AT5G29000 41.748 103 52 3 113 214 189 284 2.52e-16 77.8
MS.gene037788.t1 AT2G27070 26.070 257 118 7 16 219 45 282 3.45e-16 78.6
MS.gene037788.t1 AT5G29000 42.157 102 51 3 114 214 147 241 3.70e-16 77.8
MS.gene037788.t1 AT5G29000 42.157 102 51 3 114 214 147 241 3.70e-16 77.8
MS.gene037788.t1 AT5G29000 41.748 103 52 3 114 215 190 285 4.00e-16 77.8
MS.gene037788.t1 AT5G18240 43.137 102 50 3 145 245 29 123 4.88e-16 77.4
MS.gene037788.t1 AT5G18240 43.137 102 50 3 145 245 29 123 4.88e-16 77.4
MS.gene037788.t1 AT5G18240 43.137 102 50 3 145 245 29 123 5.30e-16 77.4
MS.gene037788.t1 AT5G18240 55.556 72 30 2 145 215 29 99 5.45e-16 77.4
MS.gene037788.t1 AT5G18240 55.556 72 30 2 145 215 29 99 5.45e-16 77.4
MS.gene037788.t1 AT3G04030 61.818 55 20 1 162 215 45 99 6.39e-16 77.0
MS.gene037788.t1 AT3G04030 61.818 55 20 1 162 215 45 99 6.56e-16 77.0
MS.gene037788.t1 AT3G46640 44.681 94 49 2 138 231 123 213 3.76e-15 74.3
MS.gene037788.t1 AT3G46640 44.681 94 49 2 138 231 123 213 3.76e-15 74.3
MS.gene037788.t1 AT3G46640 44.681 94 49 2 138 231 123 213 4.02e-15 74.3
MS.gene037788.t1 AT3G04030 57.407 54 23 0 162 215 45 98 6.67e-15 74.3
MS.gene037788.t1 AT5G59570 53.333 60 28 0 160 219 139 198 1.91e-14 72.0
MS.gene037788.t1 AT5G59570 53.333 60 28 0 160 219 139 198 1.91e-14 72.0
MS.gene037788.t1 AT5G05090 55.932 59 26 0 161 219 80 138 1.93e-14 71.6
MS.gene037788.t1 AT3G10760 55.932 59 26 0 161 219 104 162 3.63e-14 71.6
MS.gene037788.t1 AT3G04450 53.448 58 26 1 158 214 234 291 4.67e-14 72.0
MS.gene037788.t1 AT3G04450 53.448 58 26 1 158 214 194 251 5.35e-14 71.6
MS.gene037788.t1 AT3G04450 53.448 58 26 1 158 214 194 251 5.35e-14 71.6
MS.gene037788.t1 AT3G04450 53.448 58 26 1 158 214 234 291 6.36e-14 70.9
MS.gene037788.t1 AT2G40970 54.237 59 27 0 161 219 104 162 6.92e-14 70.1
MS.gene037788.t1 AT5G44190 56.667 60 26 0 159 218 144 203 8.14e-14 70.9
MS.gene037788.t1 AT5G44190 56.667 60 26 0 159 218 144 203 1.03e-13 70.5
MS.gene037788.t1 AT2G01060 48.193 83 41 2 151 232 4 85 1.04e-13 70.1
MS.gene037788.t1 AT4G28610 60.000 55 21 1 162 215 225 279 2.13e-13 69.7
MS.gene037788.t1 AT2G40260 44.318 88 45 2 128 214 51 135 4.40e-13 68.9
MS.gene037788.t1 AT4G13640 40.594 101 41 3 162 261 37 119 5.41e-13 68.2
MS.gene037788.t1 AT4G13640 40.594 101 41 3 162 261 37 119 5.65e-13 67.8
MS.gene037788.t1 AT2G20570 49.275 69 35 0 150 218 141 209 7.82e-13 68.2
MS.gene037788.t1 AT3G24120 38.235 102 54 2 158 250 37 138 8.63e-13 67.4
MS.gene037788.t1 AT3G24120 38.235 102 54 2 158 250 37 138 8.76e-13 67.4
MS.gene037788.t1 AT1G69580 54.545 55 24 1 162 215 34 88 9.54e-13 67.8
MS.gene037788.t1 AT1G69580 54.545 55 24 1 162 215 34 88 1.02e-12 67.4
MS.gene037788.t1 AT3G12730 36.441 118 71 3 143 258 3 118 1.23e-12 66.2
MS.gene037788.t1 AT1G14600 61.538 52 19 1 164 214 25 76 1.84e-12 65.9
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 1.90e-12 67.4
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 1.90e-12 67.4
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 1.90e-12 67.4
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 1.90e-12 67.4
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 1.90e-12 67.4
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 1.90e-12 67.4
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 2.03e-12 67.0
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 2.03e-12 67.0
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 2.03e-12 67.0
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 2.03e-12 67.0
MS.gene037788.t1 AT4G18020 40.816 98 54 2 153 250 288 381 2.03e-12 67.0
MS.gene037788.t1 AT5G06800 58.182 55 22 1 162 215 192 246 2.84e-12 65.9
MS.gene037788.t1 AT1G79430 42.857 91 46 2 158 247 30 115 3.95e-12 65.9
MS.gene037788.t1 AT5G06800 58.182 55 22 1 162 215 192 246 4.90e-12 65.9
MS.gene037788.t1 AT5G06800 58.182 55 22 1 162 215 192 246 5.10e-12 65.9
MS.gene037788.t1 AT3G13040 53.571 56 25 1 161 215 240 295 6.82e-12 65.5
MS.gene037788.t1 AT3G13040 53.571 56 25 1 161 215 240 295 6.82e-12 65.5
MS.gene037788.t1 AT3G13040 53.571 56 25 1 161 215 240 295 6.82e-12 65.5
MS.gene037788.t1 AT2G20570 58.182 55 23 0 164 218 171 225 7.53e-12 65.5
MS.gene037788.t1 AT1G25550 53.226 62 28 1 161 221 208 269 9.22e-12 64.7
MS.gene037788.t1 AT4G04605 53.571 56 25 1 160 214 12 67 1.26e-11 63.2
MS.gene037788.t1 AT2G20400 59.259 54 21 1 162 214 231 284 1.32e-11 64.7
MS.gene037788.t1 AT2G20400 59.259 54 21 1 162 214 231 284 1.36e-11 64.3
MS.gene037788.t1 AT2G42660 45.312 64 34 1 152 214 40 103 5.08e-11 62.0
MS.gene037788.t1 AT5G45580 54.545 55 24 1 162 215 7 61 8.08e-11 61.2
MS.gene037788.t1 AT2G38300 48.214 56 28 1 160 214 52 107 8.14e-11 62.0
MS.gene037788.t1 AT2G03500 48.387 62 31 1 161 221 232 293 9.84e-11 62.0

Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGCATCAACGATTTCTA+TGG 0.228648 4.3:-76791183 MS.gene037788:CDS
CAAAATGATTCTCAAAATTA+TGG 0.243357 4.3:-76791036 MS.gene037788:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4.3:-76790991 MS.gene037788:intron
ATCCTACCTTTACAACCTTA+TGG 0.307805 4.3:-76790702 MS.gene037788:CDS
CTCAAAATTATGGATGTTCC+TGG 0.317981 4.3:-76791026 MS.gene037788:CDS
CTTGAATTTGTTGGAAAGAA+AGG 0.326280 4.3:-76791660 MS.gene037788:CDS
AGAAATCGTTGATGCAATTC+TGG 0.327125 4.3:+76791187 None:intergenic
ATGACAGGAATGTTGAGTTT+TGG 0.342313 4.3:+76791563 None:intergenic
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4.3:-76791669 MS.gene037788:CDS
AAAGTGACTAACCTTGATAA+TGG 0.378712 4.3:+76791827 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4.3:-76791396 MS.gene037788:CDS
AATTATGGATGTTCCTGGTT+TGG 0.386251 4.3:-76791021 MS.gene037788:CDS
ATTATGGATGTTCCTGGTTT+GGG 0.388266 4.3:-76791020 MS.gene037788:CDS
CTTTCTTTCCAACAAATTCA+AGG 0.396613 4.3:+76791661 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4.3:-76791304 MS.gene037788:CDS
TGATCAAGAAAATAGAAAAC+GGG 0.418887 4.3:-76791272 MS.gene037788:CDS
TGGCAACATGTTATAAGAAA+AGG 0.422808 4.3:-76791337 MS.gene037788:CDS
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4.3:-76790862 MS.gene037788:intron
CAAAGAAGGCCCGTTTGTCT+TGG 0.431338 4.3:-76791213 MS.gene037788:CDS
ATATGAAATTGTTGGAAGCT+TGG 0.432524 4.3:-76791296 MS.gene037788:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4.3:-76791227 MS.gene037788:CDS
TCTATGGGCAGTCAATCAAC+TGG 0.447610 4.3:-76791167 MS.gene037788:CDS
TGTAATCGATTCCATTATCA+AGG 0.450836 4.3:-76791838 MS.gene037788:intron
AATAAATTCTTACTGATGAC+AGG 0.459071 4.3:+76791548 None:intergenic
CTGAATGGATTGATCAGAAT+CGG 0.460255 4.3:-76790766 MS.gene037788:CDS
ACAATGACTTTCTAGCTGAA+TGG 0.460839 4.3:-76790742 MS.gene037788:CDS
TTCTGGCAACCAAGACAAAC+GGG 0.461199 4.3:+76791204 None:intergenic
GCGATAACCTTGCGACCAGC+AGG 0.467197 4.3:+76791904 None:intergenic
AAGTCATAAGCATCCATGTT+TGG 0.471983 4.3:+76791602 None:intergenic
TTGATCAAGAAAATAGAAAA+CGG 0.477078 4.3:-76791273 MS.gene037788:CDS
TTGCGACCAGCAGGGAATGA+TGG 0.478583 4.3:+76791913 None:intergenic
GATGTTCCTGGTTTGGGTGT+AGG 0.487511 4.3:-76791014 MS.gene037788:CDS
GAATGGATTGACTGTGGGGC+GGG 0.487587 4.3:-76790725 MS.gene037788:CDS
TAGCTGAATGGATTGACTGT+GGG 0.492334 4.3:-76790730 MS.gene037788:CDS
ATTCTGGCAACCAAGACAAA+CGG 0.495052 4.3:+76791203 None:intergenic
TAAACGGCATCTAGGCCATA+AGG 0.496018 4.3:+76790687 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4.3:-76791412 MS.gene037788:CDS
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4.3:-76790843 MS.gene037788:CDS
GTGATAGCTCTCAAGCTGAA+TGG 0.519299 4.3:-76790781 MS.gene037788:CDS
AGGAAAGAAGCATACTATCA+AGG 0.523468 4.3:+76791143 None:intergenic
TTATGAAGTCTATTGAATGT+GGG 0.532174 4.3:-76791411 MS.gene037788:CDS
TGCGACCAGCAGGGAATGAT+GGG 0.540406 4.3:+76791914 None:intergenic
CACTGTTCTCAACACTATTG+AGG 0.542505 4.3:-76791866 MS.gene037788:CDS
ATCATTCCCTGCTGGTCGCA+AGG 0.546510 4.3:-76791911 MS.gene037788:CDS
CTTCATAATAGAACTTTCTG+TGG 0.551255 4.3:+76791426 None:intergenic
TCTAGGCCATAAGGTTGTAA+AGG 0.556671 4.3:+76790696 None:intergenic
ATTGCATCAACGATTTCTAT+GGG 0.557361 4.3:-76791182 MS.gene037788:CDS
GACAACCCATCATTCCCTGC+TGG 0.565580 4.3:-76791919 MS.gene037788:CDS
CTAGCTGAATGGATTGACTG+TGG 0.582882 4.3:-76790731 MS.gene037788:CDS
AACAGTGATGTCATGATCAA+TGG 0.586818 4.3:+76791882 None:intergenic
TCTTATAACATGTTGCCACA+TGG 0.594344 4.3:+76791342 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4.3:-76791410 MS.gene037788:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4.3:+76790700 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4.3:-76791615 MS.gene037788:CDS
AGGGAATGATGGGTTGTCAA+CGG 0.609008 4.3:+76791924 None:intergenic
ACATGTCCTACACCCAAACC+AGG 0.611300 4.3:+76791008 None:intergenic
AGCTGAATGGATTGACTGTG+GGG 0.620097 4.3:-76790729 MS.gene037788:CDS
CTATGGGCAGTCAATCAACT+GGG 0.621290 4.3:-76791166 MS.gene037788:CDS
TCGGCTGACAAAACAAAACA+CGG 0.641210 4.3:-76790824 MS.gene037788:CDS
CGATAACCTTGCGACCAGCA+GGG 0.652172 4.3:+76791905 None:intergenic
TGAATGGATTGACTGTGGGG+CGG 0.657142 4.3:-76790726 MS.gene037788:CDS
CGGCTGACAAAACAAAACAC+GGG 0.658379 4.3:-76790823 MS.gene037788:CDS
GATCAAGAAAATAGAAAACG+GGG 0.722642 4.3:-76791271 MS.gene037788:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG - chr4.3:76791327-76791346 MS.gene037788:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG + chr4.3:76791596-76791615 None:intergenic 15.0%
!! CAAAATGATTCTCAAAATTA+TGG - chr4.3:76791595-76791614 MS.gene037788:CDS 20.0%
!! TTGATCAAGAAAATAGAAAA+CGG - chr4.3:76791358-76791377 MS.gene037788:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG + chr4.3:76791597-76791616 None:intergenic 20.0%
! AATAAATTCTTACTGATGAC+AGG + chr4.3:76791086-76791105 None:intergenic 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr4.3:76791511-76791530 None:intergenic 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4.3:76791219-76791238 MS.gene037788:CDS 25.0%
! TGATCAAGAAAATAGAAAAC+GGG - chr4.3:76791359-76791378 MS.gene037788:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4.3:76791220-76791239 MS.gene037788:CDS 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG - chr4.3:76791173-76791192 MS.gene037788:CDS 25.0%
!!! TTGAACTGTTTTTCAACAAA+AGG + chr4.3:76791267-76791286 None:intergenic 25.0%
AAAAGAAACACTTGCAAAGA+AGG - chr4.3:76791404-76791423 MS.gene037788:CDS 30.0%
AAAGTGACTAACCTTGATAA+TGG + chr4.3:76790807-76790826 None:intergenic 30.0%
AATTGCATCAACGATTTCTA+TGG - chr4.3:76791448-76791467 MS.gene037788:intron 30.0%
ATATGAAATTGTTGGAAGCT+TGG - chr4.3:76791335-76791354 MS.gene037788:CDS 30.0%
ATTGCATCAACGATTTCTAT+GGG - chr4.3:76791449-76791468 MS.gene037788:intron 30.0%
CTTCATAATAGAACTTTCTG+TGG + chr4.3:76791208-76791227 None:intergenic 30.0%
CTTTCTTTCCAACAAATTCA+AGG + chr4.3:76790973-76790992 None:intergenic 30.0%
GATCAAGAAAATAGAAAACG+GGG - chr4.3:76791360-76791379 MS.gene037788:CDS 30.0%
TAGGATTCATTTGAAGAGAT+CGG - chr4.3:76791788-76791807 MS.gene037788:intron 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4.3:76791221-76791240 MS.gene037788:CDS 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4.3:76791294-76791313 MS.gene037788:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG - chr4.3:76791769-76791788 MS.gene037788:intron 30.0%
! TGTAATCGATTCCATTATCA+AGG - chr4.3:76790793-76790812 MS.gene037788:CDS 30.0%
!! CTTGAATTTGTTGGAAAGAA+AGG - chr4.3:76790971-76790990 MS.gene037788:intron 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG - chr4.3:76791651-76791670 MS.gene037788:CDS 30.0%
AAAAACAGTTCAAGACCATG+TGG - chr4.3:76791274-76791293 MS.gene037788:CDS 35.0%
AACAGTGATGTCATGATCAA+TGG + chr4.3:76790752-76790771 None:intergenic 35.0%
AAGTCATAAGCATCCATGTT+TGG + chr4.3:76791032-76791051 None:intergenic 35.0%
AATTATGGATGTTCCTGGTT+TGG - chr4.3:76791610-76791629 MS.gene037788:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr4.3:76791640-76791659 MS.gene037788:CDS 35.0%
ACGGGAAGAAAAAAACTAAG+TGG - chr4.3:76791826-76791845 MS.gene037788:intron 35.0%
AGAAATCGTTGATGCAATTC+TGG + chr4.3:76791447-76791466 None:intergenic 35.0%
ATCAGAAGCTGTGTTACATT+TGG + chr4.3:76790949-76790968 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG - chr4.3:76791929-76791948 MS.gene037788:CDS 35.0%
ATTATGGATGTTCCTGGTTT+GGG - chr4.3:76791611-76791630 MS.gene037788:CDS 35.0%
CTCAAAATTATGGATGTTCC+TGG - chr4.3:76791605-76791624 MS.gene037788:CDS 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4.3:76791292-76791311 None:intergenic 35.0%
TTCTGATGCCTTGAATTTGT+TGG - chr4.3:76790962-76790981 MS.gene037788:intron 35.0%
! AGGAAAGAAGCATACTATCA+AGG + chr4.3:76791491-76791510 None:intergenic 35.0%
! ATGACAGGAATGTTGAGTTT+TGG + chr4.3:76791071-76791090 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+GGG + chr4.3:76791070-76791089 None:intergenic 35.0%
!! ACAATGACTTTCTAGCTGAA+TGG - chr4.3:76791889-76791908 MS.gene037788:CDS 35.0%
!! CTGAATGGATTGATCAGAAT+CGG - chr4.3:76791865-76791884 MS.gene037788:CDS 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG - chr4.3:76791655-76791674 MS.gene037788:CDS 35.0%
!!! TGAGTTTTGGGGTAACATTT+TGG + chr4.3:76791058-76791077 None:intergenic 35.0%
ATTCTGGCAACCAAGACAAA+CGG + chr4.3:76791431-76791450 None:intergenic 40.0%
CACTGTTCTCAACACTATTG+AGG - chr4.3:76790765-76790784 MS.gene037788:CDS 40.0%
TCGGCTGACAAAACAAAACA+CGG - chr4.3:76791807-76791826 MS.gene037788:intron 40.0%
TGATGCTCATATGCCAAACA+TGG - chr4.3:76791016-76791035 MS.gene037788:CDS 40.0%
! GACAGGAATGTTGAGTTTTG+GGG + chr4.3:76791069-76791088 None:intergenic 40.0%
!! TAGCTGAATGGATTGACTGT+GGG - chr4.3:76791901-76791920 MS.gene037788:CDS 40.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4.3:76791235-76791254 MS.gene037788:CDS 45.0%
CGGCTGACAAAACAAAACAC+GGG - chr4.3:76791808-76791827 MS.gene037788:intron 45.0%
CTATGGGCAGTCAATCAACT+GGG - chr4.3:76791465-76791484 MS.gene037788:intron 45.0%
GGCCATAAGGTTGTAAAGGT+AGG + chr4.3:76791934-76791953 None:intergenic 45.0%
GTGATAGCTCTCAAGCTGAA+TGG - chr4.3:76791850-76791869 MS.gene037788:CDS 45.0%
TCTATGGGCAGTCAATCAAC+TGG - chr4.3:76791464-76791483 MS.gene037788:intron 45.0%
TTCTGGCAACCAAGACAAAC+GGG + chr4.3:76791430-76791449 None:intergenic 45.0%
!! AGCTGAATGGATTGACTGTG+GGG - chr4.3:76791902-76791921 MS.gene037788:CDS 45.0%
!! AGGGAATGATGGGTTGTCAA+CGG + chr4.3:76790710-76790729 None:intergenic 45.0%
!! CTAGCTGAATGGATTGACTG+TGG - chr4.3:76791900-76791919 MS.gene037788:CDS 45.0%
ACATGTCCTACACCCAAACC+AGG + chr4.3:76791626-76791645 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4.3:76791418-76791437 MS.gene037788:CDS 50.0%
!! GATGTTCCTGGTTTGGGTGT+AGG - chr4.3:76791617-76791636 MS.gene037788:CDS 50.0%
!! TGAATGGATTGACTGTGGGG+CGG - chr4.3:76791905-76791924 MS.gene037788:CDS 50.0%
ATCATTCCCTGCTGGTCGCA+AGG - chr4.3:76790720-76790739 MS.gene037788:CDS 55.0%
CGATAACCTTGCGACCAGCA+GGG + chr4.3:76790729-76790748 None:intergenic 55.0%
GACAACCCATCATTCCCTGC+TGG - chr4.3:76790712-76790731 MS.gene037788:CDS 55.0%
TGCGACCAGCAGGGAATGAT+GGG + chr4.3:76790720-76790739 None:intergenic 55.0%
TTGCGACCAGCAGGGAATGA+TGG + chr4.3:76790721-76790740 None:intergenic 55.0%
!! GAATGGATTGACTGTGGGGC+GGG - chr4.3:76791906-76791925 MS.gene037788:CDS 55.0%
GCGATAACCTTGCGACCAGC+AGG + chr4.3:76790730-76790749 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 76790697 76791956 76790697 ID=MS.gene037788
chr4.3 mRNA 76790697 76791956 76790697 ID=MS.gene037788.t1;Parent=MS.gene037788
chr4.3 exon 76791839 76791956 76791839 ID=MS.gene037788.t1.exon1;Parent=MS.gene037788.t1
chr4.3 CDS 76791839 76791956 76791839 ID=cds.MS.gene037788.t1;Parent=MS.gene037788.t1
chr4.3 exon 76791561 76791692 76791561 ID=MS.gene037788.t1.exon2;Parent=MS.gene037788.t1
chr4.3 CDS 76791561 76791692 76791561 ID=cds.MS.gene037788.t1;Parent=MS.gene037788.t1
chr4.3 exon 76791158 76791463 76791158 ID=MS.gene037788.t1.exon3;Parent=MS.gene037788.t1
chr4.3 CDS 76791158 76791463 76791158 ID=cds.MS.gene037788.t1;Parent=MS.gene037788.t1
chr4.3 exon 76790992 76791068 76790992 ID=MS.gene037788.t1.exon4;Parent=MS.gene037788.t1
chr4.3 CDS 76790992 76791068 76790992 ID=cds.MS.gene037788.t1;Parent=MS.gene037788.t1
chr4.3 exon 76790697 76790870 76790697 ID=MS.gene037788.t1.exon5;Parent=MS.gene037788.t1
chr4.3 CDS 76790697 76790870 76790697 ID=cds.MS.gene037788.t1;Parent=MS.gene037788.t1
Gene Sequence

>MS.gene037788

ATGGCTGTTTCCGTTGACAACCCATCATTCCCTGCTGGTCGCAAGGTTATCGCCATTGATCATGACATCACTGTTCTCAACACTATTGAGGATATGTGTAATCGATTCCATTATCAAGGTTAGTCACTTTGTTAATTGCACTTTATTCAATTCTGAAGTTTCTATGTATGTTCTTACTTTTATTTATTTTTATGCTTGTTTATTTCTTCATTCTTATGCATATTTTTATATGTTTCTTTATTTCAGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCCAAAACTCAACATTCCTGTCATCAGTAAGAATTTATTCAACTTTACACATTTCACATGATTCATTCATATATATGTACTTAAGAAGTGTGAACTGACATTATTATTATTTCTCTTTTGTAGTGATGGCTGTTGATTCCACAGAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTTGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTAACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTTGATCAAGAAAATAGAAAACGGGGTAGAGAAGATTCTAATGTCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTTGCCAGAATTGCATCAACGATTTCTATGGGCAGTCAATCAACTGGGCCTTGATAGTATGCTTCTTTCCTAATTCTTTTCTTTGCTTTGTTTGTATTATTTAACAACTCAACTTATGTGCATTTGTATCTCACTTATCTTTCAGAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCTGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTCGGAAGGAGTCTAATTAAATTATTAATAATATACTACATGTATTTTGAGTGATTTTAAATTGAAACTTATAGAGTACTATTGTTAAAATTGTGATTGCAATGTCGTTTGCAGAAATATAGGATTCATTTGAAGAGATCGGCTGACAAAACAAAACACGGGAAGAAAAAAACTAAGTGGAGTGATAGCTCTCAAGCTGAATGGATTGATCAGAATCGGAACAATGACTTTCTAGCTGAATGGATTGACTGTGGGGCGGGATCCTACCTTTACAACCTTATGGCCTAG
Protein sequence

>MS.gene037788.t1

MAVSVDNPSFPAGRKVIAIDHDITVLNTIEDMCNRFHYQASDALNLLERKDCFDVMLIDAHMPNMDAYDFVQNVTPKLNIPVIMMAVDSTESSIMKSIECGACEYWTKPFVEKQFKTMWQHVIRKGLTENKEYEIVGSLVDQENRKRGREDSNVSKETLAKKARLSWLPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADKTKHGKKKTKWSDSSQAEWIDQNRNNDFLAEWIDCGAGSYLYNLMA