AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037868

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037868.t1 MTR_0558s0020 87.446 231 29 0 1 231 66 296 6.46e-148 431
MS.gene037868.t1 MTR_6g090505 82.700 237 41 0 1 237 1 237 1.74e-138 410
MS.gene037868.t1 MTR_6g090505 66.667 102 7 2 320 419 230 306 4.57e-33 131
MS.gene037868.t1 MTR_6g090505 80.488 41 8 0 498 538 322 362 6.01e-13 71.2
MS.gene037868.t1 MTR_1g083640 88.725 204 23 0 1 204 81 284 4.70e-131 388
MS.gene037868.t1 MTR_8g446020 78.509 228 49 0 4 231 29 256 4.98e-126 374
MS.gene037868.t1 MTR_0337s0060 58.667 300 28 3 371 670 21 224 6.84e-104 316
MS.gene037868.t1 MTR_2g014100 86.047 172 24 0 1 172 74 245 1.14e-103 316
MS.gene037868.t1 MTR_0337s0040 77.083 192 44 0 4 195 30 221 1.38e-101 310
MS.gene037868.t1 MTR_1g055240 87.324 142 18 0 27 168 3 144 2.75e-85 265
MS.gene037868.t1 MTR_0019s0290 80.921 152 29 0 18 169 9 160 3.15e-82 257
MS.gene037868.t1 MTR_3g008240 85.507 138 20 0 1 138 66 203 4.92e-79 250
MS.gene037868.t1 MTR_4g040430 83.333 138 19 1 1 138 65 198 1.34e-74 238
MS.gene037868.t1 MTR_3g028680 83.846 130 21 0 541 670 1 130 1.81e-72 233
MS.gene037868.t1 MTR_4g133495 60.656 183 46 2 491 670 80 239 1.06e-65 218
MS.gene037868.t1 MTR_4g133340 60.656 183 46 2 491 670 80 239 1.06e-65 218
MS.gene037868.t1 MTR_2g067090 79.528 127 26 0 544 670 99 225 3.42e-62 209
MS.gene037868.t1 MTR_5g071590 79.612 103 21 0 1 103 78 180 1.58e-53 182
MS.gene037868.t1 MTR_6g051425 32.967 273 155 5 21 277 64 324 6.26e-37 141
MS.gene037868.t1 MTR_1281s0010 80.952 63 12 0 1 63 47 109 2.63e-29 112
MS.gene037868.t1 MTR_1g070355 26.087 207 139 2 21 227 119 311 4.16e-14 75.9
MS.gene037868.t1 MTR_0337s0050 72.222 36 10 0 195 230 14 49 2.41e-12 63.2
MS.gene037868.t1 MTR_0402s0040 25.333 150 97 3 20 169 71 205 4.18e-12 69.3
MS.gene037868.t1 MTR_4g035820 24.268 239 159 7 16 235 89 324 7.97e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037868.t1 AT4G38180 26.244 583 353 12 20 588 118 637 1.69e-59 214
MS.gene037868.t1 AT4G15090 25.563 622 383 13 59 664 121 678 1.04e-54 201
MS.gene037868.t1 AT4G15090 25.563 622 383 13 59 664 121 678 1.04e-54 201
MS.gene037868.t1 AT4G15090 25.563 622 383 13 59 664 121 678 1.04e-54 201
MS.gene037868.t1 AT4G15090 25.563 622 383 13 59 664 121 678 1.04e-54 201
MS.gene037868.t1 AT4G15090 25.563 622 383 13 59 664 121 678 1.04e-54 201
MS.gene037868.t1 AT2G27110 24.875 599 359 15 63 642 108 634 5.17e-46 176
MS.gene037868.t1 AT2G27110 24.875 599 359 15 63 642 108 634 5.17e-46 176
MS.gene037868.t1 AT2G27110 25.636 511 306 11 149 642 36 489 2.40e-45 172
MS.gene037868.t1 AT1G76320 24.106 643 385 18 19 643 41 598 1.81e-43 167
MS.gene037868.t1 AT1G76320 24.106 643 385 18 19 643 41 598 4.70e-43 166
MS.gene037868.t1 AT1G76320 24.106 643 385 18 19 643 41 598 5.92e-43 166
MS.gene037868.t1 AT1G76320 24.106 643 385 18 19 643 41 598 5.92e-43 166
MS.gene037868.t1 AT3G22170 23.041 651 386 16 5 631 100 659 1.72e-42 165
MS.gene037868.t1 AT3G22170 23.041 651 386 16 5 631 100 659 1.72e-42 165
MS.gene037868.t1 AT3G06250 23.621 580 317 14 20 584 235 703 6.04e-42 163
MS.gene037868.t1 AT3G06250 23.621 580 317 14 20 584 235 703 6.04e-42 163
MS.gene037868.t1 AT3G06250 23.621 580 317 14 20 584 235 703 6.04e-42 163
MS.gene037868.t1 AT4G38170 26.047 430 249 14 171 585 24 399 4.39e-40 155
MS.gene037868.t1 AT5G18960 25.000 484 281 16 120 584 307 727 4.16e-37 149
MS.gene037868.t1 AT5G18960 25.000 484 281 16 120 584 307 727 4.16e-37 149
MS.gene037868.t1 AT1G10240 25.750 567 330 21 43 582 102 604 6.84e-37 147
MS.gene037868.t1 AT1G10240 25.750 567 330 21 43 582 102 604 6.84e-37 147
MS.gene037868.t1 AT5G28530 24.149 617 349 19 5 583 84 619 3.96e-36 145
MS.gene037868.t1 AT2G32250 23.348 681 377 21 5 666 68 622 9.87e-36 144
MS.gene037868.t1 AT2G32250 23.201 681 378 20 5 666 68 622 1.19e-35 144
MS.gene037868.t1 AT2G32250 23.201 681 378 20 5 666 68 622 1.19e-35 144
MS.gene037868.t1 AT2G32250 23.201 681 378 20 5 666 68 622 1.58e-35 144
MS.gene037868.t1 AT2G32250 23.201 681 378 20 5 666 68 622 1.58e-35 144
MS.gene037868.t1 AT2G32250 23.201 681 378 20 5 666 68 622 1.58e-35 144
MS.gene037868.t1 AT2G32250 23.348 681 377 21 5 666 27 581 1.60e-35 144
MS.gene037868.t1 AT2G32250 23.201 681 378 20 5 666 27 581 1.62e-35 144
MS.gene037868.t1 AT5G28530 24.316 621 339 21 5 583 84 615 2.53e-35 142
MS.gene037868.t1 AT5G28530 24.316 621 339 21 5 583 84 615 2.53e-35 142
MS.gene037868.t1 AT1G80010 24.070 457 282 14 146 585 253 661 2.27e-28 121
MS.gene037868.t1 AT1G52520 21.242 612 369 17 2 585 112 638 5.19e-27 117
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 106 369 2.33e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 438 558 1.47e-12 71.2
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 177 440 2.39e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 509 629 1.71e-12 71.2
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 163 426 2.50e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 495 615 1.73e-12 71.2
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 163 426 2.62e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 495 615 1.61e-12 71.2
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 163 426 2.62e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 495 615 1.61e-12 71.2
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 163 426 3.19e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 495 615 1.65e-12 71.2
MS.gene037868.t1 AT4G19990 27.239 268 176 5 155 407 163 426 3.19e-22 102
MS.gene037868.t1 AT4G19990 35.338 133 72 3 514 644 495 615 1.65e-12 71.2

Find 103 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGATGAGTTAACTAATT+TGG 0.156761 8.3:+80863590 MS.gene037868:CDS
AGTCGCTTCTTTGACCTAAT+TGG 0.186128 8.3:-80864722 None:intergenic
CTTTAGTCATTTCAACATTA+TGG 0.194287 8.3:+80863857 MS.gene037868:CDS
TACATTTCAGCTGCCTTTCT+TGG 0.197151 8.3:+80863309 MS.gene037868:intron
CAACAAGATTATGCTCTATT+TGG 0.198611 8.3:+80862786 MS.gene037868:CDS
CATCGTAAGAGTGTGTTATT+TGG 0.276792 8.3:+80863342 MS.gene037868:CDS
TATGGTTTGAGGTTTCTTAT+TGG 0.280533 8.3:-80863436 None:intergenic
TTCTTTGACCTAATTGGATT+TGG 0.299426 8.3:-80864716 None:intergenic
GGTTTCAAATGTTGATGTTT+TGG 0.317051 8.3:+80864151 MS.gene037868:CDS
AATCAGATTTCGACTACAAT+TGG 0.318540 8.3:+80863631 MS.gene037868:CDS
TGTTCTTGAGGCAGGGAAAA+AGG 0.322946 8.3:+80864568 MS.gene037868:CDS
GTCTTTAGTGCCAACATTCA+TGG 0.339668 8.3:-80864444 None:intergenic
ACCAGCCTGGTCTGTGTATA+TGG 0.341000 8.3:-80863454 None:intergenic
GCCGATATTTATTGTTTGTT+CGG 0.341758 8.3:-80862836 None:intergenic
GAGGGATAATCATGCTGATT+TGG 0.346844 8.3:+80863890 MS.gene037868:CDS
TATGCATTGGTCTTCAGTTT+GGG 0.370760 8.3:+80863731 MS.gene037868:CDS
GAGGAATTTCTTATTTCTAA+AGG 0.381584 8.3:+80864593 MS.gene037868:CDS
CTCAACGGTGTTCTTGAGGC+AGG 0.386173 8.3:+80864560 MS.gene037868:CDS
AACAATAAATATCGGCCATT+AGG 0.388497 8.3:+80862843 MS.gene037868:CDS
TCATGCTGATTTGGACTTCA+AGG 0.389304 8.3:+80863899 MS.gene037868:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 8.3:+80862835 MS.gene037868:CDS
GTATGCATTGGTCTTCAGTT+TGG 0.392120 8.3:+80863730 MS.gene037868:CDS
TTCTCATACTCAACAAGAAC+TGG 0.395633 8.3:-80862306 None:intergenic
AAGAAAGACCAAATCCAATT+AGG 0.397771 8.3:+80864708 MS.gene037868:CDS
CATGTCGTATGTTTGAGAAC+CGG 0.411671 8.3:+80864276 MS.gene037868:CDS
GATTATGATCTTCAAACCTT+GGG 0.413114 8.3:-80864199 None:intergenic
GGGTTGTCACAGTCGACATT+AGG 0.419786 8.3:+80864234 MS.gene037868:CDS
CGATTATGATCTTCAAACCT+TGG 0.425681 8.3:-80864200 None:intergenic
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 8.3:+80862483 MS.gene037868:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 8.3:+80862365 MS.gene037868:CDS
TGAATGTCTATAAGATATAT+AGG 0.445219 8.3:+80862397 MS.gene037868:CDS
TGGTCTGTGTATATGGTTTG+AGG 0.447350 8.3:-80863447 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 8.3:+80862348 MS.gene037868:CDS
CGCTTTAAAACATATTGTGC+AGG 0.447633 8.3:-80864377 None:intergenic
CGTTATGCTTGTAACAAAGA+AGG 0.449439 8.3:+80862231 MS.gene037868:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 8.3:+80862513 MS.gene037868:CDS
TGGTCTTTGCTCATGGCACA+TGG 0.452802 8.3:+80863521 MS.gene037868:CDS
CTCCTCAAAATAGTATTTGA+AGG 0.453936 8.3:-80864033 None:intergenic
ATGTCGTATGTTTGAGAACC+GGG 0.461344 8.3:+80864277 MS.gene037868:CDS
GGCCTACTTCAGACTTTAAG+TGG 0.462107 8.3:+80863685 MS.gene037868:CDS
CGCAAACACGTGTAGAAAGC+TGG 0.465682 8.3:-80864511 None:intergenic
ACAAAATAAGAAATCGGATA+AGG 0.469178 8.3:+80862197 MS.gene037868:CDS
TTTGAGGAGGACACAACAAA+AGG 0.469432 8.3:+80864047 MS.gene037868:CDS
TTACAACCAACCCTTTCCTC+TGG 0.485330 8.3:-80862342 None:intergenic
TCTAATGACGACTCGTCAAA+AGG 0.488508 8.3:+80862626 MS.gene037868:CDS
CAATTCACAAAATAAGAAAT+CGG 0.490263 8.3:+80862191 MS.gene037868:CDS
ATGATTGTCAAAACCAAGAA+AGG 0.491378 8.3:-80863322 None:intergenic
ATCAGATTTCGACTACAATT+GGG 0.491708 8.3:+80863632 MS.gene037868:CDS
CGTCAAAAGGAGATGGTGGC+TGG 0.492100 8.3:+80862639 MS.gene037868:CDS
ACATTATGGTTCAGAGAATG+AGG 0.494022 8.3:+80863871 MS.gene037868:CDS
AAATGACTAAAGAATCGAAC+AGG 0.494923 8.3:-80863846 None:intergenic
GACCGCATAAGCTGACTGTT+GGG 0.496307 8.3:-80863951 None:intergenic
TGTCTTCTGCAGCATCAACT+TGG 0.502977 8.3:-80862731 None:intergenic
CTCATTCAAATCGTATGCAT+TGG 0.507227 8.3:+80863718 MS.gene037868:CDS
ATCATAATCGACTTGATGAA+AGG 0.507955 8.3:+80864213 MS.gene037868:CDS
TTTAAGTCCGAAAATCGAAA+AGG 0.508080 8.3:-80862597 None:intergenic
ATTGGTCTTCAGTTTGGGTG+AGG 0.509410 8.3:+80863736 MS.gene037868:CDS
AATCGAACAGGTGAATGGTC+TGG 0.516219 8.3:-80863834 None:intergenic
GATCTTCAAACCTTGGGGAA+AGG 0.519004 8.3:-80864193 None:intergenic
CAAAATAGTATTTGAAGGAA+AGG 0.519540 8.3:-80864028 None:intergenic
AAATGCTCTACTTTATGTAA+TGG 0.520476 8.3:-80862441 None:intergenic
TCATAATCGACTTGATGAAA+GGG 0.526860 8.3:+80864214 MS.gene037868:CDS
TTGAGGAGGACACAACAAAA+GGG 0.529908 8.3:+80864048 MS.gene037868:CDS
TGTCTATAAGATATATAGGT+GGG 0.532209 8.3:+80862401 MS.gene037868:CDS
CTGAAATCTACCATGAATGT+TGG 0.534154 8.3:+80864434 MS.gene037868:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.538422 8.3:+80862242 MS.gene037868:CDS
TAAAGAATCGAACAGGTGAA+TGG 0.543954 8.3:-80863839 None:intergenic
TATATCTTATAGACATTCAA+CGG 0.544310 8.3:-80862393 None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 8.3:+80862243 MS.gene037868:CDS
CGATGTTGATTATAATGAGA+GGG 0.549585 8.3:+80864130 MS.gene037868:CDS
GAGAACATCTTATGAAGTGA+TGG 0.551307 8.3:+80862545 MS.gene037868:CDS
ACTTCAACAACGCACCAGCC+TGG 0.557139 8.3:-80863467 None:intergenic
AATGAAGCTCTCTCGAAACC+AGG 0.565261 8.3:+80862693 MS.gene037868:CDS
GGGTGAAGGTGTTGGAGGCA+CGG 0.565649 8.3:+80862566 MS.gene037868:CDS
TACACGTGTTTGCGCAGATG+CGG 0.571672 8.3:+80864520 MS.gene037868:CDS
GGACCGCATAAGCTGACTGT+TGG 0.579264 8.3:-80863952 None:intergenic
TGGTGTATACTTGTTTACTA+CGG 0.585960 8.3:-80863973 None:intergenic
TCAACGGTGTTCTTGAGGCA+GGG 0.590097 8.3:+80864561 MS.gene037868:CDS
ACCATATACACAGACCAGGC+TGG 0.590176 8.3:+80863453 MS.gene037868:CDS
ATTTCTCAACGGTGTTCTTG+AGG 0.591378 8.3:+80864556 MS.gene037868:CDS
TGAAGACTTAGAAGGCGTAA+CGG 0.598405 8.3:-80864636 None:intergenic
TTGAAGGAAACCTTTCCCCA+AGG 0.600566 8.3:+80864183 MS.gene037868:CDS
GAAGTGATGGGTGAAGGTGT+TGG 0.603704 8.3:+80862558 MS.gene037868:CDS
GACGACTCGTCAAAAGGAGA+TGG 0.604288 8.3:+80862632 MS.gene037868:CDS
ACGTGTTTGCGCAGATGCGG+AGG 0.605934 8.3:+80864523 MS.gene037868:CDS
ATCCCAACAGTCAGCTTATG+CGG 0.606153 8.3:+80863949 MS.gene037868:CDS
GACTCGTCAAAAGGAGATGG+TGG 0.611157 8.3:+80862635 MS.gene037868:CDS
AGCCACTTAAAGTCTGAAGT+AGG 0.617789 8.3:-80863687 None:intergenic
AGAACTGGACTGTCACATGT+TGG 0.620791 8.3:-80862291 None:intergenic
GTGATGGGTGAAGGTGTTGG+AGG 0.621481 8.3:+80862561 MS.gene037868:CDS
ACGATGTTGATTATAATGAG+AGG 0.622271 8.3:+80864129 MS.gene037868:CDS
ATTATGATCTTCAAACCTTG+GGG 0.630358 8.3:-80864198 None:intergenic
GGTCTTTGCTCATGGCACAT+GGG 0.639768 8.3:+80863522 MS.gene037868:CDS
AGAACATCTTATGAAGTGAT+GGG 0.643044 8.3:+80862546 MS.gene037868:CDS
CATTATGGTTCAGAGAATGA+GGG 0.643278 8.3:+80863872 MS.gene037868:CDS
ATGTCTATAAGATATATAGG+TGG 0.643489 8.3:+80862400 MS.gene037868:CDS
GATTTCAGCTTATCGACAGA+CGG 0.644926 8.3:-80864419 None:intergenic
TGAATAAATGAAAAGCAAGA+TGG 0.655697 8.3:-80863993 None:intergenic
TCTTATGAAGTGATGGGTGA+AGG 0.656961 8.3:+80862552 MS.gene037868:CDS
GCATCTGAAATATTTCTCAA+CGG 0.661104 8.3:+80864545 MS.gene037868:CDS
CATGTCGACATAAGAAACCC+CGG 0.663279 8.3:-80864295 None:intergenic
TCAAACCATATACACAGACC+AGG 0.671057 8.3:+80863449 MS.gene037868:CDS
TGTCGTATGTTTGAGAACCG+GGG 0.673760 8.3:+80864278 MS.gene037868:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTCACAAAATAAGAAAT+CGG + chr8.3:80862191-80862210 MS.gene037868:CDS 20.0%
!! TATATCTTATAGACATTCAA+CGG - chr8.3:80862396-80862415 None:intergenic 20.0%
!! TGAATGTCTATAAGATATAT+AGG + chr8.3:80862397-80862416 MS.gene037868:CDS 20.0%
!!! AATTTAATGTTCTGGTTTAT+TGG + chr8.3:80863018-80863037 MS.gene037868:intron 20.0%
!!! ATGATGAAAAACTGTTTTAA+TGG + chr8.3:80863660-80863679 MS.gene037868:CDS 20.0%
!!! TGAATGTTTATATTGATTGA+AGG + chr8.3:80862990-80863009 MS.gene037868:intron 20.0%
! AAAACACCTTGAAAAACATT+CGG - chr8.3:80863498-80863517 None:intergenic 25.0%
! AAATGCTCTACTTTATGTAA+TGG - chr8.3:80862444-80862463 None:intergenic 25.0%
! ACAAAATAAGAAATCGGATA+AGG + chr8.3:80862197-80862216 MS.gene037868:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG + chr8.3:80862835-80862854 MS.gene037868:CDS 25.0%
! AGTACAAAATACTAACCTAA+TGG - chr8.3:80862861-80862880 None:intergenic 25.0%
! ATGTCTATAAGATATATAGG+TGG + chr8.3:80862400-80862419 MS.gene037868:CDS 25.0%
! CAAAATAGTATTTGAAGGAA+AGG - chr8.3:80864031-80864050 None:intergenic 25.0%
! CTTTAGTCATTTCAACATTA+TGG + chr8.3:80863857-80863876 MS.gene037868:CDS 25.0%
! TGAAATTTGAATGAATGTTC+TGG + chr8.3:80862908-80862927 MS.gene037868:intron 25.0%
! TGAATAAATGAAAAGCAAGA+TGG - chr8.3:80863996-80864015 None:intergenic 25.0%
! TGTCTATAAGATATATAGGT+GGG + chr8.3:80862401-80862420 MS.gene037868:CDS 25.0%
! TTTCGATGAGTTAACTAATT+TGG + chr8.3:80863590-80863609 MS.gene037868:CDS 25.0%
!! AGGTTTAGAATTTAATGTTC+TGG + chr8.3:80863010-80863029 MS.gene037868:intron 25.0%
!! CAAAGTTTTTACAATTGAGA+AGG + chr8.3:80864091-80864110 MS.gene037868:CDS 25.0%
!! TGTTGTATTTTGTTCTGATT+TGG + chr8.3:80863280-80863299 MS.gene037868:intron 25.0%
!! TTCCTTCAAATACTATTTTG+AGG + chr8.3:80864031-80864050 MS.gene037868:CDS 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG + chr8.3:80862748-80862767 MS.gene037868:CDS 25.0%
!!! AGACATAGCTAGTATTTTTT+GGG + chr8.3:80862749-80862768 MS.gene037868:CDS 25.0%
!!! CTTATTTTGTGAATTGTTCT+TGG - chr8.3:80862187-80862206 None:intergenic 25.0%
!!! GAGGAATTTCTTATTTCTAA+AGG + chr8.3:80864593-80864612 MS.gene037868:CDS 25.0%
!!! GCTTTAATAAATTCTAAAGC+TGG + chr8.3:80862513-80862532 MS.gene037868:CDS 25.0%
!!! TGAAAAACTGTTTTAATGGA+AGG + chr8.3:80863664-80863683 MS.gene037868:CDS 25.0%
AAACACATAAGATCTTTCCA+TGG - chr8.3:80863081-80863100 None:intergenic 30.0%
AAATGACTAAAGAATCGAAC+AGG - chr8.3:80863849-80863868 None:intergenic 30.0%
AACAAAGAAGGTTTCAAGAA+GGG + chr8.3:80862243-80862262 MS.gene037868:CDS 30.0%
AACAATAAATATCGGCCATT+AGG + chr8.3:80862843-80862862 MS.gene037868:CDS 30.0%
AATCAGATTTCGACTACAAT+TGG + chr8.3:80863631-80863650 MS.gene037868:CDS 30.0%
AGAACATCTTATGAAGTGAT+GGG + chr8.3:80862546-80862565 MS.gene037868:CDS 30.0%
ATCAGATTTCGACTACAATT+GGG + chr8.3:80863632-80863651 MS.gene037868:CDS 30.0%
ATGATTGTCAAAACCAAGAA+AGG - chr8.3:80863325-80863344 None:intergenic 30.0%
ATTATGATCTTCAAACCTTG+GGG - chr8.3:80864201-80864220 None:intergenic 30.0%
CAACAAGATTATGCTCTATT+TGG + chr8.3:80862786-80862805 MS.gene037868:CDS 30.0%
CACAAATTCTAAGTCAAGAT+AGG - chr8.3:80863048-80863067 None:intergenic 30.0%
CTCCTCAAAATAGTATTTGA+AGG - chr8.3:80864036-80864055 None:intergenic 30.0%
CTGGTATATGTGTGTTAATT+TGG + chr8.3:80862947-80862966 MS.gene037868:intron 30.0%
GAGAATGCAAAGAAAAATCT+CGG + chr8.3:80863546-80863565 MS.gene037868:CDS 30.0%
GATTATGATCTTCAAACCTT+GGG - chr8.3:80864202-80864221 None:intergenic 30.0%
GCCGATATTTATTGTTTGTT+CGG - chr8.3:80862839-80862858 None:intergenic 30.0%
TAAAGAAAAACCAGAGGAAA+GGG + chr8.3:80862332-80862351 MS.gene037868:CDS 30.0%
TAACAAAGAAGGTTTCAAGA+AGG + chr8.3:80862242-80862261 MS.gene037868:CDS 30.0%
TGGATATCATAGTAAAAACC+TGG - chr8.3:80862714-80862733 None:intergenic 30.0%
TGGTGTATACTTGTTTACTA+CGG - chr8.3:80863976-80863995 None:intergenic 30.0%
TTTAAGTCCGAAAATCGAAA+AGG - chr8.3:80862600-80862619 None:intergenic 30.0%
TTTCTATTTACACAGCTCAA+TGG + chr8.3:80863239-80863258 MS.gene037868:intron 30.0%
! ACGATGTTGATTATAATGAG+AGG + chr8.3:80864129-80864148 MS.gene037868:CDS 30.0%
! AGCTGGAATATATTTGAAGT+TGG + chr8.3:80862365-80862384 MS.gene037868:CDS 30.0%
! CGATGTTGATTATAATGAGA+GGG + chr8.3:80864130-80864149 MS.gene037868:CDS 30.0%
! GAATCTTCCTTTTCGATTTT+CGG + chr8.3:80862590-80862609 MS.gene037868:CDS 30.0%
! GCATCTGAAATATTTCTCAA+CGG + chr8.3:80864545-80864564 MS.gene037868:CDS 30.0%
! GGTTTCAAATGTTGATGTTT+TGG + chr8.3:80864151-80864170 MS.gene037868:CDS 30.0%
! TATGGTTTGAGGTTTCTTAT+TGG - chr8.3:80863439-80863458 None:intergenic 30.0%
! TCAATTCCGAATGTTTTTCA+AGG + chr8.3:80863489-80863508 MS.gene037868:intron 30.0%
! TTTGGTCTTTCTTTGAACTT+TGG - chr8.3:80864701-80864720 None:intergenic 30.0%
!! ATCATAATCGACTTGATGAA+AGG + chr8.3:80864213-80864232 MS.gene037868:CDS 30.0%
!! ATGTTCTGGTTTAGAATTGA+AGG + chr8.3:80862922-80862941 MS.gene037868:intron 30.0%
!! CTTCAAATACTATTTTGAGG+AGG + chr8.3:80864034-80864053 MS.gene037868:CDS 30.0%
!! TCATAATCGACTTGATGAAA+GGG + chr8.3:80864214-80864233 MS.gene037868:CDS 30.0%
!! TCGACATGTTTTCAAGATTT+TGG + chr8.3:80864310-80864329 MS.gene037868:CDS 30.0%
!!! CTGCACAATATGTTTTAAAG+CGG + chr8.3:80864378-80864397 MS.gene037868:CDS 30.0%
!!! GTTTTTCAAGGTGTTTTTCA+TGG + chr8.3:80863501-80863520 MS.gene037868:intron 30.0%
!!! TTCAAGATTTTGGAGTTCTT+AGG + chr8.3:80864320-80864339 MS.gene037868:CDS 30.0%
ACATTATGGTTCAGAGAATG+AGG + chr8.3:80863871-80863890 MS.gene037868:CDS 35.0%
CATTATGGTTCAGAGAATGA+GGG + chr8.3:80863872-80863891 MS.gene037868:CDS 35.0%
CGAGAAGTAAATGAAGTTCA+AGG + chr8.3:80862483-80862502 MS.gene037868:CDS 35.0%
CGATTATGATCTTCAAACCT+TGG - chr8.3:80864203-80864222 None:intergenic 35.0%
CGTTATGCTTGTAACAAAGA+AGG + chr8.3:80862231-80862250 MS.gene037868:CDS 35.0%
CTAAAGAAAAACCAGAGGAA+AGG + chr8.3:80862331-80862350 MS.gene037868:CDS 35.0%
CTCATTCAAATCGTATGCAT+TGG + chr8.3:80863718-80863737 MS.gene037868:CDS 35.0%
CTGAAATCTACCATGAATGT+TGG + chr8.3:80864434-80864453 MS.gene037868:CDS 35.0%
GAAAGCTAAAGAAAAACCAG+AGG + chr8.3:80862326-80862345 MS.gene037868:CDS 35.0%
GAGAACATCTTATGAAGTGA+TGG + chr8.3:80862545-80862564 MS.gene037868:CDS 35.0%
GTCTGCTTAATCCTAAAAAC+AGG + chr8.3:80862268-80862287 MS.gene037868:CDS 35.0%
TAAAGAATCGAACAGGTGAA+TGG - chr8.3:80863842-80863861 None:intergenic 35.0%
TTCTCATACTCAACAAGAAC+TGG - chr8.3:80862309-80862328 None:intergenic 35.0%
! CATCGTAAGAGTGTGTTATT+TGG + chr8.3:80863342-80863361 MS.gene037868:CDS 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG + chr8.3:80863801-80863820 MS.gene037868:CDS 35.0%
! TTTTTCATGGTCTTTGCTCA+TGG + chr8.3:80863514-80863533 MS.gene037868:CDS 35.0%
!! ACTGCTTTTGAAGACTTAGA+AGG - chr8.3:80864647-80864666 None:intergenic 35.0%
!! CGCTTTAAAACATATTGTGC+AGG - chr8.3:80864380-80864399 None:intergenic 35.0%
!! TATGCATTGGTCTTCAGTTT+GGG + chr8.3:80863731-80863750 MS.gene037868:CDS 35.0%
!!! AAGATTTTGGAGTTCTTAGG+TGG + chr8.3:80864323-80864342 MS.gene037868:CDS 35.0%
!!! AGACATCAGCTACTTTTGAT+TGG + chr8.3:80863385-80863404 MS.gene037868:CDS 35.0%
!!! CACAATATGTTTTAAAGCGG+TGG + chr8.3:80864381-80864400 MS.gene037868:CDS 35.0%
!!! GCTAGTATTTTTTGGGCATA+TGG + chr8.3:80862756-80862775 MS.gene037868:CDS 35.0%
ACCTATGATCTTTGCATAGC+TGG - chr8.3:80863179-80863198 None:intergenic 40.0%
ATGTCGTATGTTTGAGAACC+GGG + chr8.3:80864277-80864296 MS.gene037868:CDS 40.0%
ATTTCTCAACGGTGTTCTTG+AGG + chr8.3:80864556-80864575 MS.gene037868:CDS 40.0%
CATGTCGTATGTTTGAGAAC+CGG + chr8.3:80864276-80864295 MS.gene037868:CDS 40.0%
GATTTCAGCTTATCGACAGA+CGG - chr8.3:80864422-80864441 None:intergenic 40.0%
GTCTTTAGTGCCAACATTCA+TGG - chr8.3:80864447-80864466 None:intergenic 40.0%
TACATTTCAGCTGCCTTTCT+TGG + chr8.3:80863309-80863328 MS.gene037868:intron 40.0%
TCAAACCATATACACAGACC+AGG + chr8.3:80863449-80863468 MS.gene037868:CDS 40.0%
TCCAGCTATGCAAAGATCAT+AGG + chr8.3:80863175-80863194 MS.gene037868:intron 40.0%
TCTAATGACGACTCGTCAAA+AGG + chr8.3:80862626-80862645 MS.gene037868:CDS 40.0%
TGGTCTGTGTATATGGTTTG+AGG - chr8.3:80863450-80863469 None:intergenic 40.0%
TTGAGGAGGACACAACAAAA+GGG + chr8.3:80864048-80864067 MS.gene037868:CDS 40.0%
TTTGAGGAGGACACAACAAA+AGG + chr8.3:80864047-80864066 MS.gene037868:CDS 40.0%
! AGCCACTTAAAGTCTGAAGT+AGG - chr8.3:80863690-80863709 None:intergenic 40.0%
! GAGGGATAATCATGCTGATT+TGG + chr8.3:80863890-80863909 MS.gene037868:CDS 40.0%
! TGAAGACTTAGAAGGCGTAA+CGG - chr8.3:80864639-80864658 None:intergenic 40.0%
! TGGTTTAGAATTGAAGGCTC+TGG + chr8.3:80862928-80862947 MS.gene037868:intron 40.0%
!! GTATGCATTGGTCTTCAGTT+TGG + chr8.3:80863730-80863749 MS.gene037868:CDS 40.0%
!! GTTTTGGATGCAGAACTTGA+AGG + chr8.3:80864167-80864186 MS.gene037868:CDS 40.0%
!! TCACATGTTGGCCTGTTTTT+AGG - chr8.3:80862282-80862301 None:intergenic 40.0%
!! TCATGCTGATTTGGACTTCA+AGG + chr8.3:80863899-80863918 MS.gene037868:CDS 40.0%
!! TCTTATGAAGTGATGGGTGA+AGG + chr8.3:80862552-80862571 MS.gene037868:CDS 40.0%
AATCGAACAGGTGAATGGTC+TGG - chr8.3:80863837-80863856 None:intergenic 45.0%
AATGAAGCTCTCTCGAAACC+AGG + chr8.3:80862693-80862712 MS.gene037868:CDS 45.0%
AGAACTGGACTGTCACATGT+TGG - chr8.3:80862294-80862313 None:intergenic 45.0%
ATCCCAACAGTCAGCTTATG+CGG + chr8.3:80863949-80863968 MS.gene037868:CDS 45.0%
ATTTGTGCTCTGTACAGCCA+TGG + chr8.3:80863061-80863080 MS.gene037868:intron 45.0%
CATGTCGACATAAGAAACCC+CGG - chr8.3:80864298-80864317 None:intergenic 45.0%
GAAAAACCAGAGGAAAGGGT+TGG + chr8.3:80862336-80862355 MS.gene037868:CDS 45.0%
GATCTTCAAACCTTGGGGAA+AGG - chr8.3:80864196-80864215 None:intergenic 45.0%
GGCCTACTTCAGACTTTAAG+TGG + chr8.3:80863685-80863704 MS.gene037868:CDS 45.0%
TGTCGTATGTTTGAGAACCG+GGG + chr8.3:80864278-80864297 MS.gene037868:CDS 45.0%
TGTCTTCTGCAGCATCAACT+TGG - chr8.3:80862734-80862753 None:intergenic 45.0%
TGTTCTTGAGGCAGGGAAAA+AGG + chr8.3:80864568-80864587 MS.gene037868:CDS 45.0%
TTACAACCAACCCTTTCCTC+TGG - chr8.3:80862345-80862364 None:intergenic 45.0%
TTGAAGGAAACCTTTCCCCA+AGG + chr8.3:80864183-80864202 MS.gene037868:CDS 45.0%
! GTGAGGTCACATTTTACAGC+TGG + chr8.3:80863753-80863772 MS.gene037868:CDS 45.0%
!! ATTGGTCTTCAGTTTGGGTG+AGG + chr8.3:80863736-80863755 MS.gene037868:CDS 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG + chr8.3:80862348-80862367 MS.gene037868:CDS 45.0%
ACCAGCCTGGTCTGTGTATA+TGG - chr8.3:80863457-80863476 None:intergenic 50.0%
ACCATATACACAGACCAGGC+TGG + chr8.3:80863453-80863472 MS.gene037868:CDS 50.0%
CGCAAACACGTGTAGAAAGC+TGG - chr8.3:80864514-80864533 None:intergenic 50.0%
CTGTCTGTGAGCCATCTGTT+GGG + chr8.3:80864669-80864688 MS.gene037868:intron 50.0%
GACCGCATAAGCTGACTGTT+GGG - chr8.3:80863954-80863973 None:intergenic 50.0%
GACGACTCGTCAAAAGGAGA+TGG + chr8.3:80862632-80862651 MS.gene037868:CDS 50.0%
GACTCGTCAAAAGGAGATGG+TGG + chr8.3:80862635-80862654 MS.gene037868:CDS 50.0%
GGGTTGTCACAGTCGACATT+AGG + chr8.3:80864234-80864253 MS.gene037868:CDS 50.0%
GGTCTTTGCTCATGGCACAT+GGG + chr8.3:80863522-80863541 MS.gene037868:CDS 50.0%
TACACGTGTTTGCGCAGATG+CGG + chr8.3:80864520-80864539 MS.gene037868:CDS 50.0%
TCAACGGTGTTCTTGAGGCA+GGG + chr8.3:80864561-80864580 MS.gene037868:CDS 50.0%
TGGTCTTTGCTCATGGCACA+TGG + chr8.3:80863521-80863540 MS.gene037868:CDS 50.0%
! TTTGGTGCAGTCCCAACAGA+TGG - chr8.3:80864683-80864702 None:intergenic 50.0%
!! GAAGTGATGGGTGAAGGTGT+TGG + chr8.3:80862558-80862577 MS.gene037868:CDS 50.0%
ACTTCAACAACGCACCAGCC+TGG - chr8.3:80863470-80863489 None:intergenic 55.0%
CTCAACGGTGTTCTTGAGGC+AGG + chr8.3:80864560-80864579 MS.gene037868:CDS 55.0%
GCTGTCTGTGAGCCATCTGT+TGG + chr8.3:80864668-80864687 MS.gene037868:intron 55.0%
GGACCGCATAAGCTGACTGT+TGG - chr8.3:80863955-80863974 None:intergenic 55.0%
TGAGGCAGGGAAAAAGGCAG+AGG + chr8.3:80864574-80864593 MS.gene037868:CDS 55.0%
! CGTCAAAAGGAGATGGTGGC+TGG + chr8.3:80862639-80862658 MS.gene037868:CDS 55.0%
!! GTGATGGGTGAAGGTGTTGG+AGG + chr8.3:80862561-80862580 MS.gene037868:CDS 55.0%
ACGTGTTTGCGCAGATGCGG+AGG + chr8.3:80864523-80864542 MS.gene037868:CDS 60.0%
!! GGGTGAAGGTGTTGGAGGCA+CGG + chr8.3:80862566-80862585 MS.gene037868:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 80862174 80864729 80862174 ID=MS.gene037868
chr8.3 mRNA 80862174 80864729 80862174 ID=MS.gene037868.t1;Parent=MS.gene037868
chr8.3 exon 80862174 80862864 80862174 ID=MS.gene037868.t1.exon1;Parent=MS.gene037868.t1
chr8.3 CDS 80862174 80862864 80862174 ID=cds.MS.gene037868.t1;Parent=MS.gene037868.t1
chr8.3 exon 80863319 80863474 80863319 ID=MS.gene037868.t1.exon2;Parent=MS.gene037868.t1
chr8.3 CDS 80863319 80863474 80863319 ID=cds.MS.gene037868.t1;Parent=MS.gene037868.t1
chr8.3 exon 80863511 80864674 80863511 ID=MS.gene037868.t1.exon3;Parent=MS.gene037868.t1
chr8.3 CDS 80863511 80864674 80863511 ID=cds.MS.gene037868.t1;Parent=MS.gene037868.t1
chr8.3 exon 80864704 80864729 80864704 ID=MS.gene037868.t1.exon4;Parent=MS.gene037868.t1
chr8.3 CDS 80864704 80864729 80864704 ID=cds.MS.gene037868.t1;Parent=MS.gene037868.t1
Gene Sequence

>MS.gene037868

ATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAACCGTTGAATGTCTATAAGATATATAGGTGGGTAGTTGATCACTGTCATCCATTACATAAAGTAGAGCATTTGCGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTGAAGGTGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACGACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGCATATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGGTTAGTATTTTGTACTTCTCACAGTTTTTAATTCTTATATTCTTGAAATTTGAATGAATGTTCTGGTTTAGAATTGAAGGCTCTGGTATATGTGTGTTAATTTGGCTCTTGTGTGTGTTTAGAATTGAATGTTTATATTGATTGAAGGTTTAGAATTTAATGTTCTGGTTTATTGGTGATCCTATCTTGACTTAGAATTTGTGCTCTGTACAGCCATGGAAAGATCTTATGTGTTTTTTTATCGATGATAAATAAATAGCTCTTTCTGTGTTTGCGAGTTTAGACATCTCGCTTGAGAAGTTGTGCTATGTCCAGCTATGCAAAGATCATAGGTGTTTTTTTGTGTTTTTTTACAGTTGTTAAATGAATAGCTTTTTCTATTTACACAGCTCAATGGTTTTATATTTTTTAAATATGTTGTATTTTGTTCTGATTTGGTTCATTTACATTTCAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCAGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACCTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGATTTGGACTTCAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTTTGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTTACAATTGAGAAGGTTGATTATAATTACGACGATGTTGATTATAATGAGAGGGTTTCAAATGTTGATGTTTTGGATGCAGAACTTGAAGGAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTAGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGTTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCTACCATGAATGTTGGCACTAAAGACAACACTCAAGCACAACGATATCAACAAATTTGTGCTGTTACAGTCCAGCTTTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAATTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTGTGAGCCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAG
Protein sequence

>MS.gene037868.t1

MLKSKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEPLNVYKIYRWVVDHCHPLHKVEHLRYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGEGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWAYGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSPVRFFSHFNIMVQRMRDNHADLDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYFEEDTTKGSELNKFFKVFTIEKVDYNYDDVDYNERVSNVDVLDAELEGNLSPRFEDHNRLDERVVTVDIRSKSFTCSCRMFENRGFLCRHVFKILEFLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDNTQAQRYQQICAVTVQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVFQRKTKSN