Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038513.t1 | KEH21988.1 | 62.8 | 234 | 35 | 3 | 1 | 233 | 1 | 183 | 8.50E-39 | 170.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038513.t1 | A0A072TYR1 | 62.8 | 234 | 35 | 3 | 1 | 233 | 1 | 183 | 6.1e-39 | 170.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene038513.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038513.t1 | MTR_7g028855 | 61.905 | 231 | 36 | 5 | 1 | 230 | 1 | 180 | 4.93e-77 | 239 |
| MS.gene038513.t1 | MTR_7g446480 | 58.031 | 193 | 31 | 3 | 1 | 193 | 1 | 143 | 5.13e-60 | 191 |
| MS.gene038513.t1 | MTR_7g446520 | 60.920 | 174 | 28 | 2 | 57 | 230 | 15 | 148 | 6.21e-60 | 192 |
| MS.gene038513.t1 | MTR_8g467420 | 49.315 | 73 | 37 | 0 | 153 | 225 | 61 | 133 | 7.86e-20 | 83.6 |
| MS.gene038513.t1 | MTR_3g080590 | 50.000 | 58 | 29 | 0 | 163 | 220 | 98 | 155 | 1.27e-12 | 63.9 |
| MS.gene038513.t1 | MTR_3g448650 | 44.262 | 61 | 34 | 0 | 166 | 226 | 78 | 138 | 5.38e-12 | 62.8 |
| MS.gene038513.t1 | MTR_1g070355 | 46.552 | 58 | 31 | 0 | 172 | 229 | 76 | 133 | 1.77e-11 | 63.5 |
| MS.gene038513.t1 | MTR_6g072520 | 44.628 | 121 | 46 | 3 | 1 | 120 | 1 | 101 | 4.69e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATGACTTTACGGATTT+TGG | 0.057115 | 8.4:-36696299 | MS.gene038513:CDS |
| CATTAACTGCTGAAGGAATA+AGG | 0.236547 | 8.4:-36695818 | MS.gene038513:CDS |
| CCACCACTCCCAACAATTAT+CGG | 0.301918 | 8.4:+36696221 | None:intergenic |
| AGTGATGATGGTGGTTGTTC+TGG | 0.317361 | 8.4:-36696068 | MS.gene038513:CDS |
| AAACAACAACAATGACTTTA+CGG | 0.325210 | 8.4:-36696306 | MS.gene038513:CDS |
| TTGTTAGGAAGAGTGATATT+AGG | 0.334249 | 8.4:-36695725 | MS.gene038513:CDS |
| GGTACACCGGAACATGATTA+CGG | 0.407134 | 8.4:-36696278 | MS.gene038513:CDS |
| TGCTGAAGGAATAAGGGAAA+TGG | 0.412429 | 8.4:-36695811 | MS.gene038513:CDS |
| GATTATAGTGCTGGTGATAA+AGG | 0.421954 | 8.4:-36696044 | MS.gene038513:CDS |
| ATTAACTGCTGAAGGAATAA+GGG | 0.439851 | 8.4:-36695817 | MS.gene038513:CDS |
| GAGAGAAGAAGCATTTATCA+AGG | 0.445750 | 8.4:-36695644 | MS.gene038513:intron |
| GATATTAGGCGCAATGTTAA+CGG | 0.449906 | 8.4:-36695711 | MS.gene038513:CDS |
| TATAAGTACGGTAAGTGTAA+AGG | 0.460291 | 8.4:-36695753 | MS.gene038513:CDS |
| GAAACATATCCGATAATTGT+TGG | 0.467655 | 8.4:-36696230 | MS.gene038513:CDS |
| TGATTACGGTTCTACGGATG+TGG | 0.481654 | 8.4:-36696264 | MS.gene038513:CDS |
| TGTTCTGGTGATTATAGTGC+TGG | 0.489646 | 8.4:-36696053 | MS.gene038513:CDS |
| AAACATATCCGATAATTGTT+GGG | 0.492862 | 8.4:-36696229 | MS.gene038513:CDS |
| GTAGAACCGTAATCATGTTC+CGG | 0.492886 | 8.4:+36696272 | None:intergenic |
| GCAGTATGTATGCAACAAGC+AGG | 0.502107 | 8.4:-36695673 | MS.gene038513:CDS |
| TGTAGTTGTTGGTGAGGAAA+TGG | 0.504666 | 8.4:-36695853 | MS.gene038513:CDS |
| GAAGATGATGATGTAGTTGT+TGG | 0.505754 | 8.4:-36695864 | MS.gene038513:CDS |
| AAGAATACTCAAGGGAATAC+AGG | 0.512005 | 8.4:-36696125 | MS.gene038513:CDS |
| ATAAATTCATTAACTGCTGA+AGG | 0.519495 | 8.4:-36695825 | MS.gene038513:CDS |
| CCGATAATTGTTGGGAGTGG+TGG | 0.529659 | 8.4:-36696221 | MS.gene038513:CDS |
| GGAACATGATTACGGTTCTA+CGG | 0.552732 | 8.4:-36696270 | MS.gene038513:CDS |
| TTGTTGGTGAGGAAATGGTA+AGG | 0.572827 | 8.4:-36695848 | MS.gene038513:CDS |
| GATGAAAGTCAAGGTGATGA+AGG | 0.579998 | 8.4:-36695999 | MS.gene038513:CDS |
| CAGTATGTATGCAACAAGCA+GGG | 0.584535 | 8.4:-36695672 | MS.gene038513:CDS |
| GGTGATAAAGGTGAAGATGA+TGG | 0.589452 | 8.4:-36696032 | MS.gene038513:CDS |
| TATCCGATAATTGTTGGGAG+TGG | 0.600518 | 8.4:-36696224 | MS.gene038513:CDS |
| GATGAAGGTGAAAGTGATGA+AGG | 0.602180 | 8.4:-36695984 | MS.gene038513:CDS |
| AGAGACAATGAAAGTGATGA+TGG | 0.611273 | 8.4:-36696080 | MS.gene038513:CDS |
| AGTGAAGGTCATGGTAGTGA+AGG | 0.614950 | 8.4:-36695903 | MS.gene038513:CDS |
| AGTGAAGGTCATGGTAGTGA+AGG | 0.614950 | 8.4:-36695948 | MS.gene038513:CDS |
| AGTATGTATGCAACAAGCAG+GGG | 0.634341 | 8.4:-36695671 | MS.gene038513:CDS |
| TGATGATGTAGTTGTTGGTG+AGG | 0.665725 | 8.4:-36695859 | MS.gene038513:CDS |
| GGTGATGAAAGTGAAGGTCA+TGG | 0.667300 | 8.4:-36695957 | MS.gene038513:CDS |
| GATGATGATGATGTGAGTCA+TGG | 0.679569 | 8.4:-36696158 | MS.gene038513:CDS |
| TTAACGGTGAGATTGTAATG+AGG | 0.680367 | 8.4:-36695695 | MS.gene038513:CDS |
| GACAATGAAAGTGATGATGG+TGG | 0.702934 | 8.4:-36696077 | MS.gene038513:CDS |
| GGTGCTGAAAGTGAAGGTCA+TGG | 0.708520 | 8.4:-36695912 | MS.gene038513:CDS |
| AGTGAATGTGATGAAAGTCA+AGG | 0.717258 | 8.4:-36696008 | MS.gene038513:CDS |
| GGTGAAAGTGATGAAGGTGA+AGG | 0.723043 | 8.4:-36695978 | MS.gene038513:CDS |
| AGTGAAGGTGCTGAAAGTGA+AGG | 0.724477 | 8.4:-36695918 | MS.gene038513:CDS |
| AGTGAAGGTGATGAAAGTGA+AGG | 0.731903 | 8.4:-36695933 | MS.gene038513:CDS |
| GGTGAAGGTGATGAAAGTGA+AGG | 0.739796 | 8.4:-36695963 | MS.gene038513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACGATTTTTATTATAAGTA+CGG | - | chr8.4:36696065-36696084 | MS.gene038513:CDS | 15.0% |
| ! | AAACAACAACAATGACTTTA+CGG | - | chr8.4:36695524-36695543 | MS.gene038513:intron | 25.0% |
| ! | AAACATATCCGATAATTGTT+GGG | - | chr8.4:36695601-36695620 | MS.gene038513:intron | 25.0% |
| ! | ATAAATTCATTAACTGCTGA+AGG | - | chr8.4:36696005-36696024 | MS.gene038513:CDS | 25.0% |
| !! | AATTTTACGAAGAATACTCA+AGG | - | chr8.4:36695696-36695715 | MS.gene038513:CDS | 25.0% |
| !! | ATTTTACGAAGAATACTCAA+GGG | - | chr8.4:36695697-36695716 | MS.gene038513:CDS | 25.0% |
| AGCATTTATCAAGGTTAGAT+TGG | - | chr8.4:36696195-36696214 | MS.gene038513:CDS | 30.0% | |
| ATTAACTGCTGAAGGAATAA+GGG | - | chr8.4:36696013-36696032 | MS.gene038513:CDS | 30.0% | |
| GAAACATATCCGATAATTGT+TGG | - | chr8.4:36695600-36695619 | MS.gene038513:intron | 30.0% | |
| TATAAGTACGGTAAGTGTAA+AGG | - | chr8.4:36696077-36696096 | MS.gene038513:CDS | 30.0% | |
| TTGTTAGGAAGAGTGATATT+AGG | - | chr8.4:36696105-36696124 | MS.gene038513:CDS | 30.0% | |
| ! | AACAATGACTTTACGGATTT+TGG | - | chr8.4:36695531-36695550 | MS.gene038513:intron | 30.0% |
| ! | ACAACATATCTACCCTTTTT+GGG | + | chr8.4:36696284-36696303 | None:intergenic | 30.0% |
| !!! | AGTGTAAAGGTTTTGTTGTT+AGG | - | chr8.4:36696090-36696109 | MS.gene038513:CDS | 30.0% |
| !!! | GTTGTGTCTATTTTTGAAGA+GGG | - | chr8.4:36696299-36696318 | MS.gene038513:CDS | 30.0% |
| !!! | TGTTGTGTCTATTTTTGAAG+AGG | - | chr8.4:36696298-36696317 | MS.gene038513:CDS | 30.0% |
| AAGAATACTCAAGGGAATAC+AGG | - | chr8.4:36695705-36695724 | MS.gene038513:CDS | 35.0% | |
| AGAGACAATGAAAGTGATGA+TGG | - | chr8.4:36695750-36695769 | MS.gene038513:CDS | 35.0% | |
| AGTGAATGTGATGAAAGTCA+AGG | - | chr8.4:36695822-36695841 | MS.gene038513:CDS | 35.0% | |
| CATTAACTGCTGAAGGAATA+AGG | - | chr8.4:36696012-36696031 | MS.gene038513:CDS | 35.0% | |
| GATATTAGGCGCAATGTTAA+CGG | - | chr8.4:36696119-36696138 | MS.gene038513:CDS | 35.0% | |
| GTTCACTACAAACCCAAAAA+GGG | - | chr8.4:36696269-36696288 | MS.gene038513:CDS | 35.0% | |
| TGTTCACTACAAACCCAAAA+AGG | - | chr8.4:36696268-36696287 | MS.gene038513:CDS | 35.0% | |
| TTAACGGTGAGATTGTAATG+AGG | - | chr8.4:36696135-36696154 | MS.gene038513:CDS | 35.0% | |
| ! | CACAACATATCTACCCTTTT+TGG | + | chr8.4:36696285-36696304 | None:intergenic | 35.0% |
| ! | GAAGATGATGATGTAGTTGT+TGG | - | chr8.4:36695966-36695985 | MS.gene038513:CDS | 35.0% |
| !! | GAGAGAAGAAGCATTTATCA+AGG | - | chr8.4:36696186-36696205 | MS.gene038513:CDS | 35.0% |
| !! | GATTATAGTGCTGGTGATAA+AGG | - | chr8.4:36695786-36695805 | MS.gene038513:CDS | 35.0% |
| !!! | TTTTGGGTTTGTAGTGAACA+CGG | + | chr8.4:36696268-36696287 | None:intergenic | 35.0% |
| AGTATGTATGCAACAAGCAG+GGG | - | chr8.4:36696159-36696178 | MS.gene038513:CDS | 40.0% | |
| AGTGAAGGTGATGAAAGTGA+AGG | - | chr8.4:36695897-36695916 | MS.gene038513:CDS | 40.0% | |
| CAGTATGTATGCAACAAGCA+GGG | - | chr8.4:36696158-36696177 | MS.gene038513:CDS | 40.0% | |
| GATGAAAGTCAAGGTGATGA+AGG | - | chr8.4:36695831-36695850 | MS.gene038513:CDS | 40.0% | |
| GATGAAGGTGAAAGTGATGA+AGG | - | chr8.4:36695846-36695865 | MS.gene038513:CDS | 40.0% | |
| GGAACATGATTACGGTTCTA+CGG | - | chr8.4:36695560-36695579 | MS.gene038513:intron | 40.0% | |
| GGTGATAAAGGTGAAGATGA+TGG | - | chr8.4:36695798-36695817 | MS.gene038513:CDS | 40.0% | |
| GTAGAACCGTAATCATGTTC+CGG | + | chr8.4:36695561-36695580 | None:intergenic | 40.0% | |
| TATCCGATAATTGTTGGGAG+TGG | - | chr8.4:36695606-36695625 | MS.gene038513:intron | 40.0% | |
| TGCTGAAGGAATAAGGGAAA+TGG | - | chr8.4:36696019-36696038 | MS.gene038513:CDS | 40.0% | |
| TGTTCTGGTGATTATAGTGC+TGG | - | chr8.4:36695777-36695796 | MS.gene038513:CDS | 40.0% | |
| ! | GATGATGATGATGTGAGTCA+TGG | - | chr8.4:36695672-36695691 | MS.gene038513:CDS | 40.0% |
| ! | TGATGATGTAGTTGTTGGTG+AGG | - | chr8.4:36695971-36695990 | MS.gene038513:CDS | 40.0% |
| ! | TGTAGTTGTTGGTGAGGAAA+TGG | - | chr8.4:36695977-36695996 | MS.gene038513:CDS | 40.0% |
| ! | TTGTTGGTGAGGAAATGGTA+AGG | - | chr8.4:36695982-36696001 | MS.gene038513:CDS | 40.0% |
| !! | CTTTACGGATTTTGGTACAC+CGG | - | chr8.4:36695539-36695558 | MS.gene038513:intron | 40.0% |
| !! | GACAATGAAAGTGATGATGG+TGG | - | chr8.4:36695753-36695772 | MS.gene038513:CDS | 40.0% |
| ACTTTGTACGAGTGAGACGT+CGG | + | chr8.4:36696232-36696251 | None:intergenic | 45.0% | |
| CCACCACTCCCAACAATTAT+CGG | + | chr8.4:36695612-36695631 | None:intergenic | 45.0% | |
| GCAGTATGTATGCAACAAGC+AGG | - | chr8.4:36696157-36696176 | MS.gene038513:CDS | 45.0% | |
| GGTACACCGGAACATGATTA+CGG | - | chr8.4:36695552-36695571 | MS.gene038513:intron | 45.0% | |
| GGTGAAAGTGATGAAGGTGA+AGG | - | chr8.4:36695852-36695871 | MS.gene038513:CDS | 45.0% | |
| GGTGAAGGTGATGAAAGTGA+AGG | - | chr8.4:36695867-36695886 | MS.gene038513:CDS | 45.0% | |
| GGTGATGAAAGTGAAGGTCA+TGG | - | chr8.4:36695873-36695892 | MS.gene038513:CDS | 45.0% | |
| TGATTACGGTTCTACGGATG+TGG | - | chr8.4:36695566-36695585 | MS.gene038513:intron | 45.0% | |
| ! | AGTGAAGGTCATGGTAGTGA+AGG | - | chr8.4:36695882-36695901 | MS.gene038513:CDS | 45.0% |
| !! | AGTGAAGGTGCTGAAAGTGA+AGG | - | chr8.4:36695912-36695931 | MS.gene038513:CDS | 45.0% |
| !! | AGTGATGATGGTGGTTGTTC+TGG | - | chr8.4:36695762-36695781 | MS.gene038513:CDS | 45.0% |
| CCGATAATTGTTGGGAGTGG+TGG | - | chr8.4:36695609-36695628 | MS.gene038513:intron | 50.0% | |
| ! | CTCGTACAAAGTGTCCAGCT+AGG | - | chr8.4:36696240-36696259 | MS.gene038513:CDS | 50.0% |
| ! | GGTGCTGAAAGTGAAGGTCA+TGG | - | chr8.4:36695918-36695937 | MS.gene038513:CDS | 50.0% |
| TAGTGAACACGGAGCCTAGC+TGG | + | chr8.4:36696257-36696276 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 36695519 | 36696333 | 36695519 | ID=MS.gene038513 |
| chr8.4 | mRNA | 36695519 | 36696333 | 36695519 | ID=MS.gene038513.t1;Parent=MS.gene038513 |
| chr8.4 | exon | 36695645 | 36696333 | 36695645 | ID=MS.gene038513.t1.exon1;Parent=MS.gene038513.t1 |
| chr8.4 | CDS | 36695645 | 36696333 | 36695645 | ID=cds.MS.gene038513.t1;Parent=MS.gene038513.t1 |
| chr8.4 | exon | 36695519 | 36695534 | 36695519 | ID=MS.gene038513.t1.exon2;Parent=MS.gene038513.t1 |
| chr8.4 | CDS | 36695519 | 36695534 | 36695519 | ID=cds.MS.gene038513.t1;Parent=MS.gene038513.t1 |
| Gene Sequence |
| Protein sequence |
