AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038527

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038527.t1 MTR_7g115350 83.459 133 21 1 113 244 242 374 1.63e-62 226
MS.gene038527.t1 MTR_8g027685 52.846 123 51 2 344 459 852 974 1.43e-29 127
MS.gene038527.t1 MTR_1g073740 93.548 62 4 0 550 611 392 453 5.84e-29 124
MS.gene038527.t1 MTR_5g010730 54.808 104 36 1 400 492 433 536 4.63e-26 114
MS.gene038527.t1 MTR_5g010730 54.808 104 36 1 400 492 488 591 5.66e-26 114
MS.gene038527.t1 MTR_4g009960 57.732 97 38 1 350 443 861 957 1.32e-25 114
MS.gene038527.t1 MTR_4g009960 57.732 97 38 1 350 443 861 957 3.08e-25 113
MS.gene038527.t1 MTR_4g009960 57.732 97 38 1 350 443 861 957 3.86e-25 112
MS.gene038527.t1 MTR_4g098890 91.667 48 4 0 610 657 497 544 1.13e-22 105
MS.gene038527.t1 MTR_8g055840 89.583 48 5 0 610 657 494 541 1.64e-22 103
MS.gene038527.t1 MTR_5g040360 79.167 48 10 0 610 657 493 540 3.27e-20 95.9
MS.gene038527.t1 MTR_3g040370 72.917 48 13 0 610 657 491 538 1.46e-18 90.9
MS.gene038527.t1 MTR_8g100075 68.750 48 15 0 610 657 514 561 4.32e-17 86.3
MS.gene038527.t1 MTR_1g101370 72.917 48 13 0 610 657 511 558 7.21e-17 85.5
MS.gene038527.t1 MTR_0019s0120 68.750 48 15 0 610 657 520 567 2.05e-16 84.0
MS.gene038527.t1 MTR_7g076170 64.583 48 17 0 610 657 519 566 5.57e-16 82.4
MS.gene038527.t1 MTR_7g006060 68.750 48 15 0 610 657 462 509 2.18e-15 80.5
MS.gene038527.t1 MTR_4g122160 41.304 92 53 1 402 492 482 573 3.67e-15 80.1
MS.gene038527.t1 MTR_4g122160 51.471 68 33 0 425 492 506 573 4.68e-15 79.7
MS.gene038527.t1 MTR_8g107000 39.091 110 54 2 404 500 753 862 1.02e-14 79.0
MS.gene038527.t1 MTR_8g432680 62.500 48 18 0 610 657 524 571 2.22e-14 77.4
MS.gene038527.t1 MTR_2g034020 38.043 92 56 1 402 492 488 579 2.43e-14 77.4
MS.gene038527.t1 MTR_3g096480 63.830 47 17 0 611 657 675 721 2.64e-14 77.4
MS.gene038527.t1 MTR_8g100160 58.442 77 22 2 276 348 413 483 2.99e-14 77.4
MS.gene038527.t1 MTR_8g100160 82.609 46 8 0 303 348 442 487 3.52e-14 77.0
MS.gene038527.t1 MTR_8g083170 82.609 46 8 0 507 552 596 641 3.56e-14 77.0
MS.gene038527.t1 MTR_6g079540 42.857 91 40 1 422 500 455 545 6.25e-14 76.3
MS.gene038527.t1 MTR_6g079540 42.857 91 40 1 422 500 703 793 7.71e-14 76.3
MS.gene038527.t1 MTR_1g016300 47.561 82 42 1 413 493 696 777 8.20e-14 75.9
MS.gene038527.t1 MTR_1g016300 47.561 82 42 1 413 493 696 777 9.88e-14 75.5
MS.gene038527.t1 MTR_5g021100 59.574 47 19 0 611 657 734 780 1.68e-13 74.7
MS.gene038527.t1 MTR_8g093880 57.447 47 20 0 611 657 705 751 3.08e-13 73.9
MS.gene038527.t1 MTR_8g076650 67.442 43 14 0 611 653 789 831 3.98e-13 73.6
MS.gene038527.t1 MTR_1g081790 38.462 91 55 1 404 493 713 803 4.40e-13 73.6
MS.gene038527.t1 MTR_1g081790 38.462 91 55 1 404 493 713 803 4.57e-13 73.6
MS.gene038527.t1 MTR_3g084470 65.385 52 18 0 507 558 589 640 4.70e-13 73.2
MS.gene038527.t1 MTR_3g088790 62.791 43 16 0 611 653 641 683 8.83e-13 72.4
MS.gene038527.t1 MTR_3g071530 55.319 47 21 0 611 657 549 595 1.66e-12 71.6
MS.gene038527.t1 MTR_5g066490 40.860 93 41 2 422 500 689 781 1.70e-12 71.6
MS.gene038527.t1 MTR_1g022255 60.976 41 16 0 611 651 597 637 2.92e-12 70.9
MS.gene038527.t1 MTR_3g084450 73.913 46 12 0 507 552 385 430 3.58e-12 70.1
MS.gene038527.t1 MTR_1g051055 63.265 49 15 1 612 657 496 544 4.65e-12 70.1
MS.gene038527.t1 MTR_3g084450 73.913 46 12 0 507 552 593 638 5.02e-12 70.1
MS.gene038527.t1 MTR_5g080720 57.447 47 20 0 611 657 535 581 6.00e-12 69.7
MS.gene038527.t1 MTR_5g026930 45.161 62 34 0 596 657 471 532 6.16e-12 69.7
MS.gene038527.t1 MTR_3g100090 35.714 112 57 3 404 500 677 788 6.21e-12 69.7
MS.gene038527.t1 MTR_8g432620 54.717 53 18 1 611 657 489 541 9.88e-12 68.9
MS.gene038527.t1 MTR_7g076150 54.717 53 18 1 611 657 333 385 1.29e-11 68.2
MS.gene038527.t1 MTR_7g076150 54.717 53 18 1 611 657 476 528 1.43e-11 68.2
MS.gene038527.t1 MTR_5g083150 53.191 47 22 0 611 657 501 547 1.49e-11 68.2
MS.gene038527.t1 MTR_8g071280 53.191 47 22 0 611 657 484 530 2.49e-11 67.4
MS.gene038527.t1 MTR_1g029730 57.143 49 19 1 611 657 552 600 2.95e-11 67.4
MS.gene038527.t1 MTR_2g066850 85.000 40 6 0 1 40 133 172 3.03e-11 67.8
MS.gene038527.t1 MTR_3g068990 55.319 47 21 0 611 657 563 609 4.07e-11 67.0
MS.gene038527.t1 MTR_1g442840 38.144 97 57 2 562 657 346 440 5.74e-11 66.2
MS.gene038527.t1 MTR_1g442840 38.144 97 57 2 562 657 459 553 6.97e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038527.t1 AT1G01320 48.980 147 66 4 320 459 846 990 4.86e-30 129
MS.gene038527.t1 AT4G28080 55.556 117 45 2 350 459 886 1002 4.03e-29 125
MS.gene038527.t1 AT1G01320 52.459 122 51 2 345 459 861 982 4.51e-29 125
MS.gene038527.t1 AT1G01320 52.459 122 51 2 345 459 861 982 4.51e-29 125
MS.gene038527.t1 AT1G15290 57.292 96 38 1 351 443 843 938 3.10e-24 110
MS.gene038527.t1 AT1G15290 65.000 80 28 0 351 430 843 922 3.20e-24 109
MS.gene038527.t1 AT4G23050 46.739 92 48 1 402 492 490 581 2.60e-19 93.6
MS.gene038527.t1 AT4G23050 46.739 92 48 1 402 492 489 580 2.62e-19 93.6
MS.gene038527.t1 AT1G26850 77.083 48 11 0 610 657 502 549 6.17e-19 92.0
MS.gene038527.t1 AT1G26850 77.083 48 11 0 610 657 502 549 6.17e-19 92.0
MS.gene038527.t1 AT4G18030 76.596 47 11 0 611 657 498 544 2.01e-18 90.5
MS.gene038527.t1 AT1G33170 68.750 48 15 0 610 657 528 575 1.64e-16 84.3
MS.gene038527.t1 AT4G10440 70.833 48 14 0 610 657 510 557 2.01e-16 84.0
MS.gene038527.t1 AT2G43200 66.667 48 16 0 610 657 505 552 1.48e-15 81.3
MS.gene038527.t1 AT2G43200 66.667 48 16 0 610 657 505 552 1.48e-15 81.3
MS.gene038527.t1 AT4G00750 64.583 48 17 0 610 657 516 563 1.65e-15 81.3
MS.gene038527.t1 AT1G67890 40.404 99 56 2 402 497 509 607 1.98e-15 81.3
MS.gene038527.t1 AT1G67890 40.404 99 56 2 402 497 381 479 2.42e-15 80.5
MS.gene038527.t1 AT1G67890 40.404 99 56 2 402 497 509 607 2.63e-15 80.5
MS.gene038527.t1 AT1G67890 40.404 99 56 2 402 497 509 607 2.63e-15 80.5
MS.gene038527.t1 AT5G49470 40.217 92 54 1 402 492 487 578 6.88e-15 79.3
MS.gene038527.t1 AT5G49470 40.217 92 54 1 402 492 386 477 6.99e-15 79.0
MS.gene038527.t1 AT5G49470 40.217 92 54 1 402 492 226 317 7.38e-15 78.6
MS.gene038527.t1 AT5G49470 40.217 92 54 1 402 492 513 604 8.89e-15 79.0
MS.gene038527.t1 AT5G49470 40.217 92 54 1 402 492 513 604 9.37e-15 79.0
MS.gene038527.t1 AT5G49470 40.217 92 54 1 402 492 513 604 9.39e-15 79.0
MS.gene038527.t1 AT5G11850 42.857 91 51 1 404 493 633 723 9.62e-15 79.0
MS.gene038527.t1 AT3G06630 38.384 99 58 2 402 497 372 470 1.10e-14 78.2
MS.gene038527.t1 AT3G06630 38.144 97 57 2 404 497 458 554 1.54e-14 78.2
MS.gene038527.t1 AT3G06630 38.144 97 57 2 404 497 458 554 1.57e-14 78.2
MS.gene038527.t1 AT3G06630 38.144 97 57 2 404 497 458 554 1.57e-14 78.2
MS.gene038527.t1 AT3G06630 38.144 97 57 2 404 497 458 554 1.57e-14 78.2
MS.gene038527.t1 AT3G06630 38.144 97 57 2 404 497 458 554 1.90e-14 77.8
MS.gene038527.t1 AT3G06620 40.206 97 55 2 404 497 518 614 1.93e-14 77.8
MS.gene038527.t1 AT3G06630 38.144 97 57 2 404 497 458 554 1.96e-14 77.8
MS.gene038527.t1 AT1G29470 63.830 47 17 0 611 657 656 702 3.84e-14 77.0
MS.gene038527.t1 AT1G29470 63.830 47 17 0 611 657 656 702 3.84e-14 77.0
MS.gene038527.t1 AT2G34300 61.702 47 18 0 611 657 656 702 5.27e-14 76.6
MS.gene038527.t1 AT2G34300 61.702 47 18 0 611 657 656 702 5.27e-14 76.6
MS.gene038527.t1 AT2G34300 61.702 47 18 0 611 657 656 702 5.27e-14 76.6
MS.gene038527.t1 AT1G18160 47.826 69 36 0 425 493 761 829 5.53e-14 76.6
MS.gene038527.t1 AT1G18160 47.826 69 36 0 425 493 761 829 5.91e-14 76.6
MS.gene038527.t1 AT1G73660 39.560 91 54 1 404 493 772 862 6.45e-14 76.6
MS.gene038527.t1 AT3G51070 61.702 47 18 0 611 657 788 834 8.71e-14 75.9
MS.gene038527.t1 AT4G24480 36.522 115 55 3 400 500 694 804 9.12e-14 75.9
MS.gene038527.t1 AT4G24480 36.522 115 55 3 400 500 694 804 9.46e-14 75.9
MS.gene038527.t1 AT5G64030 58.333 48 20 0 610 657 714 761 2.02e-13 74.7
MS.gene038527.t1 AT2G45750 60.417 48 19 0 610 657 509 556 2.16e-13 74.3
MS.gene038527.t1 AT4G24480 36.207 116 59 3 400 500 694 809 4.74e-13 73.6
MS.gene038527.t1 AT4G19120 55.319 47 21 0 611 657 486 532 9.43e-13 72.0
MS.gene038527.t1 AT4G19120 55.319 47 21 0 611 657 486 532 9.43e-13 72.0
MS.gene038527.t1 AT5G06050 57.447 47 20 0 611 657 559 605 1.47e-12 71.6
MS.gene038527.t1 AT5G03730 36.522 115 58 3 402 501 573 687 1.77e-12 71.6
MS.gene038527.t1 AT5G03730 36.522 115 58 3 402 501 573 687 1.77e-12 71.6
MS.gene038527.t1 AT3G06640 37.113 97 58 2 404 497 470 566 2.13e-12 71.2
MS.gene038527.t1 AT3G06640 37.113 97 58 2 404 497 470 566 2.13e-12 71.2
MS.gene038527.t1 AT3G06640 37.113 97 58 2 404 497 470 566 2.45e-12 71.2
MS.gene038527.t1 AT3G23790 46.535 101 44 4 507 607 594 684 2.94e-12 70.9
MS.gene038527.t1 AT4G00740 56.604 53 17 1 611 657 482 534 2.98e-12 70.5
MS.gene038527.t1 AT4G00740 56.604 53 17 1 611 657 482 534 2.98e-12 70.5
MS.gene038527.t1 AT1G77260 52.308 65 28 2 611 675 551 612 4.88e-12 70.1
MS.gene038527.t1 AT1G31850 55.319 47 21 0 611 657 492 538 5.33e-12 69.7
MS.gene038527.t1 AT1G31850 55.319 47 21 0 611 657 492 538 5.33e-12 69.7
MS.gene038527.t1 AT1G31850 55.319 47 21 0 611 657 492 538 5.33e-12 69.7
MS.gene038527.t1 AT1G31850 55.319 47 21 0 611 657 492 538 5.33e-12 69.7
MS.gene038527.t1 AT4G14070 60.656 61 19 1 507 562 610 670 5.53e-12 70.1
MS.gene038527.t1 AT5G04060 61.224 49 16 1 612 657 490 538 6.09e-12 69.7
MS.gene038527.t1 AT1G08720 36.264 91 57 1 404 493 693 783 6.75e-12 69.7
MS.gene038527.t1 AT1G08720 36.264 91 57 1 404 493 693 783 7.20e-12 69.7
MS.gene038527.t1 AT2G39750 53.191 47 22 0 611 657 587 633 8.10e-12 69.3
MS.gene038527.t1 AT3G10200 60.000 50 16 1 612 657 479 528 1.07e-11 68.9
MS.gene038527.t1 AT3G10200 60.000 50 16 1 612 657 479 528 1.15e-11 68.6
MS.gene038527.t1 AT1G19430 62.500 40 15 0 612 651 621 660 2.84e-11 67.8
MS.gene038527.t1 AT1G19430 62.500 40 15 0 612 651 621 660 2.84e-11 67.8
MS.gene038527.t1 AT3G56080 52.174 46 22 0 612 657 512 557 4.74e-11 66.6

Find 159 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACTACAATTTGGCTTTA+AGG 0.101358 8.4:+36819977 None:intergenic
TTTCCATCAGTGACTATATT+TGG 0.140336 8.4:+36819957 None:intergenic
AACCTCAAGGTCCAATATTT+TGG 0.150909 8.4:+36821462 None:intergenic
GCTTCTAGTAATTATGAAAT+TGG 0.163098 8.4:-36819903 MS.gene038527:CDS
TAGTCACAAAGTTCTTATTT+AGG 0.164503 8.4:-36820307 MS.gene038527:CDS
ACTATATTTGGACTACAATT+TGG 0.200237 8.4:+36819969 None:intergenic
AATCAGCCGGTAATTAAATT+TGG 0.201240 8.4:+36819605 None:intergenic
GACTCTGTTGACTTGGAAAA+TGG 0.218595 8.4:-36820674 MS.gene038527:CDS
ATTATGAAATTGGAACTAAA+TGG 0.234648 8.4:-36819893 MS.gene038527:CDS
AGTCACAAAGTTCTTATTTA+GGG 0.258379 8.4:-36820306 MS.gene038527:CDS
TATTCCGTCATCATCTGATA+TGG 0.266924 8.4:+36821309 None:intergenic
GGAATCATCAAAGACTTCTT+TGG 0.280837 8.4:-36820579 MS.gene038527:CDS
TTCTTTGGATAAAGGCAAAT+TGG 0.286642 8.4:-36820564 MS.gene038527:CDS
TTACCAATATCAGGTGGTTT+TGG 0.291858 8.4:+36819573 None:intergenic
TCAAAGACTTCTTTGGATAA+AGG 0.296184 8.4:-36820572 MS.gene038527:CDS
ACGCCTTTCCTGAGAATATA+TGG 0.298889 8.4:+36820334 None:intergenic
CTTATAGTCTCTTGGTTGTT+GGG 0.305073 8.4:-36820460 MS.gene038527:CDS
GATTGTTGAAGCCAAAATAT+TGG 0.313259 8.4:-36821473 MS.gene038527:CDS
TACATTGTTTCGAGTTTAGC+TGG 0.314901 8.4:-36818774 MS.gene038527:CDS
AAGAATTCCATGTGAATATA+TGG 0.318120 8.4:-36821245 MS.gene038527:CDS
TACACGCCAAATTTAATTAC+CGG 0.335512 8.4:-36819611 MS.gene038527:CDS
TATGATCTTATTCATACTAA+TGG 0.336334 8.4:-36819756 MS.gene038527:CDS
TTATGAAATTGGAACTAAAT+GGG 0.336985 8.4:-36819892 MS.gene038527:CDS
AATCAACAAATTGTAGTTGT+TGG 0.340348 8.4:-36820077 MS.gene038527:CDS
CGTCGATCCGGTGGCTTCTT+CGG 0.347592 8.4:+36820164 None:intergenic
CATAAAAGAAACTTTGCTTT+TGG 0.348683 8.4:-36819925 MS.gene038527:CDS
AAATAAGAACTTTGTGACTA+TGG 0.354375 8.4:+36820310 None:intergenic
AGCTGAAGTCCTGGGCTTCC+CGG 0.354506 8.4:+36821186 None:intergenic
GCTTATAGTCTCTTGGTTGT+TGG 0.361048 8.4:-36820461 MS.gene038527:CDS
ACTCGTGGTTCTGACAATAA+TGG 0.362283 8.4:+36821151 None:intergenic
GAAAACGTATTCTTCAGATT+TGG 0.367532 8.4:+36821535 None:intergenic
CCGCCAAAACCACCTGATAT+TGG 0.368412 8.4:-36819576 MS.gene038527:CDS
TCAACTGAGGATCTGACTTC+CGG 0.372038 8.4:-36821205 MS.gene038527:CDS
TCTGCTTGCTCATCTACTTA+TGG 0.375023 8.4:-36820614 MS.gene038527:CDS
TAAGGACTTGATGAATTCAT+TGG 0.375949 8.4:+36819995 None:intergenic
CCAGAAATTACCGAGGGCTT+TGG 0.377777 8.4:+36819372 None:intergenic
CATAGGAACCATATATTCTC+AGG 0.382258 8.4:-36820342 MS.gene038527:CDS
GATTATCCATGATGTCTTCC+AGG 0.387409 8.4:+36819703 None:intergenic
TTGATGATTCCAACCGTGTT+TGG 0.388120 8.4:+36820591 None:intergenic
TGGAATTCTTGTGTCTTCCT+TGG 0.393805 8.4:+36821258 None:intergenic
GCTATATGTTCATGTGCATT+TGG 0.393934 8.4:+36819654 None:intergenic
AACAAATTGTAGTTGTTGGT+TGG 0.396289 8.4:-36820073 MS.gene038527:CDS
GTTTGAAGTGATTGAGGAAC+AGG 0.397248 8.4:-36820843 MS.gene038527:CDS
CAACTGAGGATCTGACTTCC+GGG 0.397907 8.4:-36821204 MS.gene038527:CDS
GGAACATCATTGAAGAAAAC+AGG 0.409067 8.4:+36821001 None:intergenic
CTCAAACAGGACTGTGTGTT+AGG 0.410109 8.4:-36818802 MS.gene038527:intron
ACCGAGGGCTTTGGCTTTGT+AGG 0.410617 8.4:+36819381 None:intergenic
CTCGTGGTTCTGACAATAAT+GGG 0.415353 8.4:+36821152 None:intergenic
ATATATGAAAGAGGTTTCAT+TGG 0.416524 8.4:-36819825 MS.gene038527:CDS
ACAAATTGTAGTTGTTGGTT+GGG 0.419664 8.4:-36820072 MS.gene038527:CDS
GTGCCAACATATTCACCGTT+TGG 0.428260 8.4:+36820020 None:intergenic
GCAACAATGAAAAGTGGAAA+TGG 0.431927 8.4:-36821085 MS.gene038527:CDS
CTTCCAGGTTGCATTTGTCC+TGG 0.432650 8.4:+36819718 None:intergenic
TCTGTTGTCATCCTATTGTA+AGG 0.436100 8.4:+36820485 None:intergenic
CAAAATCCAACACCGTCGTT+TGG 0.436592 8.4:-36820704 MS.gene038527:CDS
AAAGAGGTTGGATTTATGTA+TGG 0.437023 8.4:-36819867 MS.gene038527:CDS
CGAGTTTAGCTGGGGTGGTA+TGG 0.439994 8.4:-36818764 MS.gene038527:CDS
TCATTGGTATTTATCATGAC+TGG 0.440851 8.4:-36819809 MS.gene038527:CDS
AACAGAAGCTTATAGTCTCT+TGG 0.444745 8.4:-36820468 MS.gene038527:CDS
TTGAAGAAAACAGGCTCCCT+TGG 0.446375 8.4:+36821010 None:intergenic
TGAAGAAAACAGGCTCCCTT+GGG 0.446472 8.4:+36821011 None:intergenic
ATCTGACTTCCGGGAAGCCC+AGG 0.447323 8.4:-36821195 MS.gene038527:CDS
GGAGGAAGAATGTGTTGAAA+AGG 0.449896 8.4:-36820807 MS.gene038527:CDS
GGTGACGATGAATGCGATAT+TGG 0.454304 8.4:-36820758 MS.gene038527:CDS
TTGATTTATGCTACTCCTCA+TGG 0.461069 8.4:+36820094 None:intergenic
AAGTCGTGTCTGAATTCAGC+TGG 0.468104 8.4:-36821360 MS.gene038527:CDS
TCTGTTATATTTGATAATGT+AGG 0.469008 8.4:+36820980 None:intergenic
TGAAGAGTTTGAAGTGATTG+AGG 0.469664 8.4:-36820849 MS.gene038527:CDS
ACTAAATGGGGTAAAGAGGT+TGG 0.476567 8.4:-36819879 MS.gene038527:CDS
CACAGATGTCGGAAACGCTT+CGG 0.477915 8.4:+36821564 None:intergenic
TTCTTCGGCAGCAGCAGTGA+TGG 0.479897 8.4:+36820179 None:intergenic
ATCTCCTCAATACTAACAAC+AGG 0.480254 8.4:+36820953 None:intergenic
AATTCTTGCGCTATTGAAAG+AGG 0.482749 8.4:-36821608 MS.gene038527:CDS
ACATTGTTTCGAGTTTAGCT+GGG 0.484231 8.4:-36818773 MS.gene038527:CDS
GGTTGGGATAAGCGACGTCT+TGG 0.484434 8.4:-36820056 MS.gene038527:CDS
TGGTGTGTGGGCCTTACAAT+AGG 0.485730 8.4:-36820496 MS.gene038527:CDS
TCTTGTGAAGTGTCCTGAAA+AGG 0.487914 8.4:+36820279 None:intergenic
TCTTCTGGTTCTTCATTCAG+TGG 0.490937 8.4:+36820881 None:intergenic
AGAACCTGTTGTTAGTATTG+AGG 0.492750 8.4:-36820957 MS.gene038527:CDS
TACTTATGGCCAAACACGGT+TGG 0.498088 8.4:-36820600 MS.gene038527:CDS
AGTAATTGACTCTGTTGACT+TGG 0.501194 8.4:-36820681 MS.gene038527:CDS
TGGAACTAAATGGGGTAAAG+AGG 0.503441 8.4:-36819883 MS.gene038527:CDS
CAAAGTTAGTGTTGGTGTGT+GGG 0.504085 8.4:-36820508 MS.gene038527:CDS
GTCTCAACCTTGTTATTCAG+TGG 0.506391 8.4:+36821499 None:intergenic
ATGGTTTGACAGAACTTGAC+TGG 0.512573 8.4:-36821066 MS.gene038527:CDS
TTATTCAGTGGTTCTCGTTG+TGG 0.513186 8.4:+36821511 None:intergenic
GGCACTTTCAAAGTTAGTGT+TGG 0.516141 8.4:-36820516 MS.gene038527:CDS
TCAAAGTTAGTGTTGGTGTG+TGG 0.517361 8.4:-36820509 MS.gene038527:CDS
ATTCCGTCATCATCTGATAT+GGG 0.517619 8.4:+36821310 None:intergenic
CCGGATCGACGTGTTGCTAC+AGG 0.517871 8.4:-36820152 MS.gene038527:CDS
CCTGTAGCAACACGTCGATC+CGG 0.519072 8.4:+36820152 None:intergenic
TGAGGTAGCGATGTCGTTGA+CGG 0.519862 8.4:-36821425 MS.gene038527:CDS
TTGGCGGAGGTTTAATCAGC+CGG 0.524059 8.4:+36819592 None:intergenic
GAAGGTGTTATATATGAAAG+AGG 0.524341 8.4:-36819834 MS.gene038527:CDS
TTTGGATAAAGGCAAATTGG+AGG 0.527830 8.4:-36820561 MS.gene038527:CDS
CATCATGGATAATCATGTCA+TGG 0.530143 8.4:-36819694 MS.gene038527:CDS
GCAGAAGCAACAATGAAAAG+TGG 0.534764 8.4:-36821091 MS.gene038527:CDS
GCAGCTTCTTCAAGTTCAGA+TGG 0.535868 8.4:+36820116 None:intergenic
GCTGCTGCCGAAGAAGCCAC+CGG 0.536599 8.4:-36820171 MS.gene038527:CDS
GACATCGCTACCTCATCGTC+CGG 0.538728 8.4:+36821433 None:intergenic
AAACTGCATTGATGCACGCT+TGG 0.540828 8.4:+36819515 None:intergenic
CCACAAAACAAGCTATCAAA+CGG 0.543672 8.4:-36819449 MS.gene038527:CDS
TGGTTTATTTAGTTTGTACC+AGG 0.546436 8.4:-36819736 MS.gene038527:CDS
CTGCATTGATGCACGCTTGG+TGG 0.547808 8.4:+36819518 None:intergenic
AAGAGGAGATCGACTTTGCT+CGG 0.548578 8.4:-36821591 MS.gene038527:CDS
TCAGCTGGGATAACAATTCA+CGG 0.550423 8.4:-36821345 MS.gene038527:CDS
TATGAAATTGGAACTAAATG+GGG 0.551329 8.4:-36819891 MS.gene038527:CDS
ATGCAACCTGGAAGACATCA+TGG 0.553014 8.4:-36819709 MS.gene038527:CDS
TTTGGCTGTTCCACAGATGT+CGG 0.553476 8.4:+36821553 None:intergenic
ACCTACAAAGCCAAAGCCCT+CGG 0.553704 8.4:-36819382 MS.gene038527:intron
GTGATGTTTGTCTGTCCCCA+AGG 0.554875 8.4:-36821027 MS.gene038527:CDS
TTGGATAAAGGCAAATTGGA+GGG 0.559499 8.4:-36820560 MS.gene038527:CDS
TGTTTCGAGTTTAGCTGGGG+TGG 0.560186 8.4:-36818769 MS.gene038527:CDS
ATTACTCCAAACGACGGTGT+TGG 0.561985 8.4:+36820698 None:intergenic
AGCCAAAATATTGGACCTTG+AGG 0.564494 8.4:-36821464 MS.gene038527:CDS
GGAACAGGCTGAAGAAATCA+AGG 0.564973 8.4:-36820828 MS.gene038527:CDS
GGAATAAGTATACCAATTCA+CGG 0.565875 8.4:-36821292 MS.gene038527:CDS
AGTCCAAATATAGTCACTGA+TGG 0.569444 8.4:-36819960 MS.gene038527:CDS
AAGTACATAAGCTGAAGTCC+TGG 0.577144 8.4:+36821177 None:intergenic
TTGCTTTCTACTTGTACTCG+TGG 0.581902 8.4:+36821136 None:intergenic
GGAGCTATCATCGTTCCAAA+CGG 0.587816 8.4:-36820035 MS.gene038527:CDS
AAACAGGACTGTGTGTTAGG+AGG 0.589877 8.4:-36818799 MS.gene038527:intron
AGTACATAAGCTGAAGTCCT+GGG 0.590382 8.4:+36821178 None:intergenic
CGTCCGGTGTCTCAACCTCA+AGG 0.591559 8.4:+36821449 None:intergenic
GTCACAAAGTTCTTATTTAG+GGG 0.592788 8.4:-36820305 MS.gene038527:CDS
ATTTATCATGACTGGTGTGA+AGG 0.595603 8.4:-36819801 MS.gene038527:CDS
ATCATGGATAATCATGTCAT+GGG 0.596657 8.4:-36819693 MS.gene038527:CDS
AAAGAATTAAGAACTAAACT+TGG 0.596975 8.4:-36820779 MS.gene038527:CDS
ATGGTTCTTGATGTTGCTAG+AGG 0.599266 8.4:-36820221 MS.gene038527:CDS
GCGTGTAAGTTCTCATGTGG+TGG 0.600981 8.4:+36819627 None:intergenic
GAACCATATATTCTCAGGAA+AGG 0.601007 8.4:-36820337 MS.gene038527:CDS
GGACCTTGAGGTTGAGACAC+CGG 0.603940 8.4:-36821452 MS.gene038527:CDS
TTGGAGGGTTCTATCAGCTA+CGG 0.604131 8.4:-36820545 MS.gene038527:CDS
TGAGGAGATTGATGAGCACA+TGG 0.608132 8.4:-36820939 MS.gene038527:CDS
CAAAGCTCCATATATTCACA+TGG 0.620912 8.4:+36821238 None:intergenic
GTTCCAAACGGTGAATATGT+TGG 0.623727 8.4:-36820023 MS.gene038527:CDS
GGTTGCATGATTGAAATCAC+TGG 0.625697 8.4:+36820427 None:intergenic
CATGATTGAAATCACTGGTA+TGG 0.630503 8.4:+36820432 None:intergenic
CATTGTTTCGAGTTTAGCTG+GGG 0.639604 8.4:-36818772 MS.gene038527:CDS
TGATGTTTGTCTGTCCCCAA+GGG 0.640021 8.4:-36821026 MS.gene038527:CDS
CATCTACTTATGGCCAAACA+CGG 0.644750 8.4:-36820604 MS.gene038527:CDS
AGTCGTGTCTGAATTCAGCT+GGG 0.650130 8.4:-36821359 MS.gene038527:CDS
GTTCCCATATCAGATGATGA+CGG 0.650347 8.4:-36821313 MS.gene038527:CDS
TTGCAGTGTTGATTCAACTG+AGG 0.652343 8.4:-36821218 MS.gene038527:CDS
GGTTGAGACACCGGACGATG+AGG 0.652720 8.4:-36821443 MS.gene038527:CDS
GTACCAGGACAAATGCAACC+TGG 0.653643 8.4:-36819721 MS.gene038527:CDS
CGAAGCGTTTCCGACATCTG+TGG 0.656101 8.4:-36821563 MS.gene038527:CDS
TCACGGAAAAGATGATACCA+AGG 0.660959 8.4:-36821275 MS.gene038527:CDS
GTAGCAACACGTCGATCCGG+TGG 0.668247 8.4:+36820155 None:intergenic
TTCTATCAGCTACGGCACAA+AGG 0.669058 8.4:-36820537 MS.gene038527:CDS
GAAGAAAACAGGCTCCCTTG+GGG 0.672057 8.4:+36821012 None:intergenic
TGACTGAAAATCAACAGATG+CGG 0.676499 8.4:+36819413 None:intergenic
AACTTGAAGAAGCTGCCATG+AGG 0.680892 8.4:-36820109 MS.gene038527:CDS
TTGGCGTGTAAGTTCTCATG+TGG 0.682662 8.4:+36819624 None:intergenic
ACGAGAACCACTGAATAACA+AGG 0.693448 8.4:-36821506 MS.gene038527:CDS
AGATATTTCAATCTGCTATG+CGG 0.696158 8.4:-36820643 MS.gene038527:CDS
ATGTATGGTACTGTATGTGA+AGG 0.700288 8.4:-36819852 MS.gene038527:CDS
GAGTCAATTACTCCAAACGA+CGG 0.734363 8.4:+36820692 None:intergenic
ACAGGCTGAAGAAATCAAGG+AGG 0.749004 8.4:-36820825 MS.gene038527:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTACGTTTTATTTTATTT+TGG - chr8.4:36821302-36821321 MS.gene038527:CDS 10.0%
!! TATAGAAAATTACTAAGATT+TGG + chr8.4:36821270-36821289 None:intergenic 15.0%
!! AAAGAATTAAGAACTAAACT+TGG - chr8.4:36819632-36819651 MS.gene038527:CDS 20.0%
!! ATTATGAAATTGGAACTAAA+TGG - chr8.4:36820518-36820537 MS.gene038527:CDS 20.0%
!! GATAAAAATTTACCAATATC+AGG + chr8.4:36820850-36820869 None:intergenic 20.0%
!! TATGATCTTATTCATACTAA+TGG - chr8.4:36820655-36820674 MS.gene038527:CDS 20.0%
!! TTATGAAATTGGAACTAAAT+GGG - chr8.4:36820519-36820538 MS.gene038527:CDS 20.0%
!!! AAAAGCAAAGTTTCTTTTAT+GGG + chr8.4:36820487-36820506 None:intergenic 20.0%
!!! TCTGTTATATTTGATAATGT+AGG + chr8.4:36819434-36819453 None:intergenic 20.0%
! AAAAATTTACCAATATCAGG+TGG + chr8.4:36820847-36820866 None:intergenic 25.0%
! AAATAAGAACTTTGTGACTA+TGG + chr8.4:36820104-36820123 None:intergenic 25.0%
! AAGAATTCCATGTGAATATA+TGG - chr8.4:36819166-36819185 MS.gene038527:intron 25.0%
! AATCAACAAATTGTAGTTGT+TGG - chr8.4:36820334-36820353 MS.gene038527:CDS 25.0%
! ACATAAGTTCTGCTTATATA+GGG + chr8.4:36821350-36821369 None:intergenic 25.0%
! ACTATATTTGGACTACAATT+TGG + chr8.4:36820445-36820464 None:intergenic 25.0%
! ATATATGAAAGAGGTTTCAT+TGG - chr8.4:36820586-36820605 MS.gene038527:CDS 25.0%
! GCTTCTAGTAATTATGAAAT+TGG - chr8.4:36820508-36820527 MS.gene038527:CDS 25.0%
! TACATAAGTTCTGCTTATAT+AGG + chr8.4:36821351-36821370 None:intergenic 25.0%
! TATGAAATTGGAACTAAATG+GGG - chr8.4:36820520-36820539 MS.gene038527:CDS 25.0%
! TCTTTATCAAAATGAGAACT+CGG - chr8.4:36821170-36821189 MS.gene038527:CDS 25.0%
!! AGTCACAAAGTTCTTATTTA+GGG - chr8.4:36820105-36820124 MS.gene038527:CDS 25.0%
!! CAAAAGCAAAGTTTCTTTTA+TGG + chr8.4:36820488-36820507 None:intergenic 25.0%
!! CATAAAAGAAACTTTGCTTT+TGG - chr8.4:36820486-36820505 MS.gene038527:CDS 25.0%
!! TAGTCACAAAGTTCTTATTT+AGG - chr8.4:36820104-36820123 MS.gene038527:CDS 25.0%
!!! AAACTTGTTCTTTTCTATCT+AGG - chr8.4:36821500-36821519 MS.gene038527:CDS 25.0%
!!! ACAAATGTATTGCTTTTCAT+AGG - chr8.4:36820052-36820071 MS.gene038527:CDS 25.0%
!!! TTTTATTTTATTTTGGCCCA+CGG - chr8.4:36821309-36821328 MS.gene038527:CDS 25.0%
AAAGAGGTTGGATTTATGTA+TGG - chr8.4:36820544-36820563 MS.gene038527:CDS 30.0%
AACAAATTGTAGTTGTTGGT+TGG - chr8.4:36820338-36820357 MS.gene038527:CDS 30.0%
AATCAGCCGGTAATTAAATT+TGG + chr8.4:36820809-36820828 None:intergenic 30.0%
AGATATTTCAATCTGCTATG+CGG - chr8.4:36819768-36819787 MS.gene038527:CDS 30.0%
ATCATGGATAATCATGTCAT+GGG - chr8.4:36820718-36820737 MS.gene038527:CDS 30.0%
CGACAAACCTATCAAAATAA+GGG + chr8.4:36821446-36821465 None:intergenic 30.0%
CTTTATCAAAATGAGAACTC+GGG - chr8.4:36821171-36821190 MS.gene038527:CDS 30.0%
GAAAACGTATTCTTCAGATT+TGG + chr8.4:36818879-36818898 None:intergenic 30.0%
GATTGTTGAAGCCAAAATAT+TGG - chr8.4:36818938-36818957 MS.gene038527:intron 30.0%
GGAATAAGTATACCAATTCA+CGG - chr8.4:36819119-36819138 MS.gene038527:intron 30.0%
GGGTATAAGAATAATCTCTT+AGG - chr8.4:36821565-36821584 MS.gene038527:CDS 30.0%
TAAGGACTTGATGAATTCAT+TGG + chr8.4:36820419-36820438 None:intergenic 30.0%
TACACGCCAAATTTAATTAC+CGG - chr8.4:36820800-36820819 MS.gene038527:CDS 30.0%
TCAAAGACTTCTTTGGATAA+AGG - chr8.4:36819839-36819858 MS.gene038527:CDS 30.0%
TCATTGGTATTTATCATGAC+TGG - chr8.4:36820602-36820621 MS.gene038527:CDS 30.0%
TTCTTTGGATAAAGGCAAAT+TGG - chr8.4:36819847-36819866 MS.gene038527:CDS 30.0%
TTTCCATCAGTGACTATATT+TGG + chr8.4:36820457-36820476 None:intergenic 30.0%
! AATCAAAAAGTGTGTCTCTT+AGG + chr8.4:36821375-36821394 None:intergenic 30.0%
! ACAAATTGTAGTTGTTGGTT+GGG - chr8.4:36820339-36820358 MS.gene038527:CDS 30.0%
! GTCACAAAGTTCTTATTTAG+GGG - chr8.4:36820106-36820125 MS.gene038527:CDS 30.0%
! TCTTCAGTTTCTTTTTCTTC+TGG + chr8.4:36819548-36819567 None:intergenic 30.0%
!! CATCAAAAAGCATCTTAGAA+TGG - chr8.4:36820171-36820190 MS.gene038527:CDS 30.0%
!! GAAGGTGTTATATATGAAAG+AGG - chr8.4:36820577-36820596 MS.gene038527:CDS 30.0%
!! TGCAGTTTTAATAGCACAAA+TGG + chr8.4:36819303-36819322 None:intergenic 30.0%
!! TGGTTTATTTAGTTTGTACC+AGG - chr8.4:36820675-36820694 MS.gene038527:CDS 30.0%
!!! TAGTGATTTTAGCTGATAGA+TGG + chr8.4:36820008-36820027 None:intergenic 30.0%
!!! TTTATTTTATTTTGGCCCAC+GGG - chr8.4:36821310-36821329 MS.gene038527:CDS 30.0%
AACAGAAGCTTATAGTCTCT+TGG - chr8.4:36819943-36819962 MS.gene038527:CDS 35.0%
AATTCTTGCGCTATTGAAAG+AGG - chr8.4:36818803-36818822 MS.gene038527:intron 35.0%
AGAACCTGTTGTTAGTATTG+AGG - chr8.4:36819454-36819473 MS.gene038527:CDS 35.0%
AGCGACAAACTTGTGAAAAT+AGG + chr8.4:36821477-36821496 None:intergenic 35.0%
AGTCCAAATATAGTCACTGA+TGG - chr8.4:36820451-36820470 MS.gene038527:CDS 35.0%
ATCTCCTCAATACTAACAAC+AGG + chr8.4:36819461-36819480 None:intergenic 35.0%
ATGCTCCTACATTTCTTCAA+TGG - chr8.4:36821103-36821122 MS.gene038527:CDS 35.0%
ATGTATGGTACTGTATGTGA+AGG - chr8.4:36820559-36820578 MS.gene038527:CDS 35.0%
ATTCCGTCATCATCTGATAT+GGG + chr8.4:36819104-36819123 None:intergenic 35.0%
ATTTATCATGACTGGTGTGA+AGG - chr8.4:36820610-36820629 MS.gene038527:CDS 35.0%
CAAAGCTCCATATATTCACA+TGG + chr8.4:36819176-36819195 None:intergenic 35.0%
CATAGGAACCATATATTCTC+AGG - chr8.4:36820069-36820088 MS.gene038527:CDS 35.0%
CATCATGGATAATCATGTCA+TGG - chr8.4:36820717-36820736 MS.gene038527:CDS 35.0%
CATGATTGAAATCACTGGTA+TGG + chr8.4:36819982-36820001 None:intergenic 35.0%
CATGCAAGAATATCTCAAAC+AGG - chr8.4:36821596-36821615 MS.gene038527:CDS 35.0%
CCACAAAACAAGCTATCAAA+CGG - chr8.4:36820962-36820981 MS.gene038527:CDS 35.0%
CCTATCAAAATAAGGGTTTC+CGG + chr8.4:36821439-36821458 None:intergenic 35.0%
CTTATAGTCTCTTGGTTGTT+GGG - chr8.4:36819951-36819970 MS.gene038527:CDS 35.0%
GAACCATATATTCTCAGGAA+AGG - chr8.4:36820074-36820093 MS.gene038527:CDS 35.0%
GCAACAATGAAAAGTGGAAA+TGG - chr8.4:36819326-36819345 MS.gene038527:intron 35.0%
GCGACAAACCTATCAAAATA+AGG + chr8.4:36821447-36821466 None:intergenic 35.0%
GCGACAAACTTGTGAAAATA+GGG + chr8.4:36821476-36821495 None:intergenic 35.0%
GCTATATGTTCATGTGCATT+TGG + chr8.4:36820760-36820779 None:intergenic 35.0%
GGAACATCATTGAAGAAAAC+AGG + chr8.4:36819413-36819432 None:intergenic 35.0%
GGAATCATCAAAGACTTCTT+TGG - chr8.4:36819832-36819851 MS.gene038527:CDS 35.0%
TATTCCGTCATCATCTGATA+TGG + chr8.4:36819105-36819124 None:intergenic 35.0%
TCTGTTGTCATCCTATTGTA+AGG + chr8.4:36819929-36819948 None:intergenic 35.0%
TGACTGAAAATCAACAGATG+CGG + chr8.4:36821001-36821020 None:intergenic 35.0%
TGCTCCTACATTTCTTCAAT+GGG - chr8.4:36821104-36821123 MS.gene038527:CDS 35.0%
TTGATTTATGCTACTCCTCA+TGG + chr8.4:36820320-36820339 None:intergenic 35.0%
TTGGATAAAGGCAAATTGGA+GGG - chr8.4:36819851-36819870 MS.gene038527:CDS 35.0%
TTTGGATAAAGGCAAATTGG+AGG - chr8.4:36819850-36819869 MS.gene038527:CDS 35.0%
! AACCTCAAGGTCCAATATTT+TGG + chr8.4:36818952-36818971 None:intergenic 35.0%
! AAGGCTTTTCTACATACTCA+AGG - chr8.4:36820629-36820648 MS.gene038527:CDS 35.0%
! AGATTTTCTCTCAATTCTGG+TGG - chr8.4:36821410-36821429 MS.gene038527:CDS 35.0%
! AGTAATTGACTCTGTTGACT+TGG - chr8.4:36819730-36819749 MS.gene038527:CDS 35.0%
! GTATCATCTTTTCCGTGAAT+TGG + chr8.4:36819134-36819153 None:intergenic 35.0%
! TCGAGATTTTCTCTCAATTC+TGG - chr8.4:36821407-36821426 MS.gene038527:CDS 35.0%
! TGAAGAGTTTGAAGTGATTG+AGG - chr8.4:36819562-36819581 MS.gene038527:CDS 35.0%
! TTACCAATATCAGGTGGTTT+TGG + chr8.4:36820841-36820860 None:intergenic 35.0%
!! ACATTGTTTCGAGTTTAGCT+GGG - chr8.4:36821638-36821657 MS.gene038527:CDS 35.0%
!! TACATTGTTTCGAGTTTAGC+TGG - chr8.4:36821637-36821656 MS.gene038527:CDS 35.0%
!! TGGACTACAATTTGGCTTTA+AGG + chr8.4:36820437-36820456 None:intergenic 35.0%
!!! CATCTGTTGATTTTCAGTCA+CGG - chr8.4:36821001-36821020 MS.gene038527:CDS 35.0%
AAGTACATAAGCTGAAGTCC+TGG + chr8.4:36819237-36819256 None:intergenic 40.0%
ACATTTCTTCAATGGGATCC+AGG - chr8.4:36821111-36821130 MS.gene038527:CDS 40.0%
ACGAGAACCACTGAATAACA+AGG - chr8.4:36818905-36818924 MS.gene038527:intron 40.0%
ACGCCTTTCCTGAGAATATA+TGG + chr8.4:36820080-36820099 None:intergenic 40.0%
ACTAAATGGGGTAAAGAGGT+TGG - chr8.4:36820532-36820551 MS.gene038527:CDS 40.0%
AGCCAAAATATTGGACCTTG+AGG - chr8.4:36818947-36818966 MS.gene038527:intron 40.0%
AGTACATAAGCTGAAGTCCT+GGG + chr8.4:36819236-36819255 None:intergenic 40.0%
ATGGTTCTTGATGTTGCTAG+AGG - chr8.4:36820190-36820209 MS.gene038527:CDS 40.0%
ATGGTTTGACAGAACTTGAC+TGG - chr8.4:36819345-36819364 MS.gene038527:intron 40.0%
CATCTACTTATGGCCAAACA+CGG - chr8.4:36819807-36819826 MS.gene038527:CDS 40.0%
GAAAGAGATAACAAACCCGT+GGG + chr8.4:36821328-36821347 None:intergenic 40.0%
GAGTCAATTACTCCAAACGA+CGG + chr8.4:36819722-36819741 None:intergenic 40.0%
GATTATCCATGATGTCTTCC+AGG + chr8.4:36820711-36820730 None:intergenic 40.0%
GCAGAAGCAACAATGAAAAG+TGG - chr8.4:36819320-36819339 MS.gene038527:intron 40.0%
GCTTATAGTCTCTTGGTTGT+TGG - chr8.4:36819950-36819969 MS.gene038527:CDS 40.0%
GGAGGAAGAATGTGTTGAAA+AGG - chr8.4:36819604-36819623 MS.gene038527:CDS 40.0%
GGATCCCATTGAAGAAATGT+AGG + chr8.4:36821111-36821130 None:intergenic 40.0%
GGCACTTTCAAAGTTAGTGT+TGG - chr8.4:36819895-36819914 MS.gene038527:CDS 40.0%
GGTTGCATGATTGAAATCAC+TGG + chr8.4:36819987-36820006 None:intergenic 40.0%
GTTCCAAACGGTGAATATGT+TGG - chr8.4:36820388-36820407 MS.gene038527:CDS 40.0%
GTTCCCATATCAGATGATGA+CGG - chr8.4:36819098-36819117 MS.gene038527:intron 40.0%
TCACGGAAAAGATGATACCA+AGG - chr8.4:36819136-36819155 MS.gene038527:intron 40.0%
TCAGCTGGGATAACAATTCA+CGG - chr8.4:36819066-36819085 MS.gene038527:intron 40.0%
TCTGCTTGCTCATCTACTTA+TGG - chr8.4:36819797-36819816 MS.gene038527:CDS 40.0%
TGGAACTAAATGGGGTAAAG+AGG - chr8.4:36820528-36820547 MS.gene038527:CDS 40.0%
TGGAATTCTTGTGTCTTCCT+TGG + chr8.4:36819156-36819175 None:intergenic 40.0%
TTATTCAGTGGTTCTCGTTG+TGG + chr8.4:36818903-36818922 None:intergenic 40.0%
TTGCTTTCTACTTGTACTCG+TGG + chr8.4:36819278-36819297 None:intergenic 40.0%
! GTTTGAAGTGATTGAGGAAC+AGG - chr8.4:36819568-36819587 MS.gene038527:CDS 40.0%
! TATTTAGGGGAAGCCTTTTC+AGG - chr8.4:36820119-36820138 MS.gene038527:CDS 40.0%
! TCTTGTGAAGTGTCCTGAAA+AGG + chr8.4:36820135-36820154 None:intergenic 40.0%
! TTGATGATTCCAACCGTGTT+TGG + chr8.4:36819823-36819842 None:intergenic 40.0%
!! ACTCGTGGTTCTGACAATAA+TGG + chr8.4:36819263-36819282 None:intergenic 40.0%
!! CAAAGTTAGTGTTGGTGTGT+GGG - chr8.4:36819903-36819922 MS.gene038527:CDS 40.0%
!! CATTGTTTCGAGTTTAGCTG+GGG - chr8.4:36821639-36821658 MS.gene038527:CDS 40.0%
!! CTCGTGGTTCTGACAATAAT+GGG + chr8.4:36819262-36819281 None:intergenic 40.0%
!! GACTCTGTTGACTTGGAAAA+TGG - chr8.4:36819737-36819756 MS.gene038527:CDS 40.0%
!! GTCTCAACCTTGTTATTCAG+TGG + chr8.4:36818915-36818934 None:intergenic 40.0%
!! TCAAAGTTAGTGTTGGTGTG+TGG - chr8.4:36819902-36819921 MS.gene038527:CDS 40.0%
!! TCTTCTGGTTCTTCATTCAG+TGG + chr8.4:36819533-36819552 None:intergenic 40.0%
!! TTGCAGTGTTGATTCAACTG+AGG - chr8.4:36819193-36819212 MS.gene038527:intron 40.0%
!!! CCGGAAACCCTTATTTTGAT+AGG - chr8.4:36821436-36821455 MS.gene038527:CDS 40.0%
!!! CCGTTTGATAGCTTGTTTTG+TGG + chr8.4:36820965-36820984 None:intergenic 40.0%
AAACAGGACTGTGTGTTAGG+AGG - chr8.4:36821612-36821631 MS.gene038527:CDS 45.0%
AACTTGAAGAAGCTGCCATG+AGG - chr8.4:36820302-36820321 MS.gene038527:CDS 45.0%
AAGTCGTGTCTGAATTCAGC+TGG - chr8.4:36819051-36819070 MS.gene038527:intron 45.0%
ACAGGCTGAAGAAATCAAGG+AGG - chr8.4:36819586-36819605 MS.gene038527:CDS 45.0%
AGTCGTGTCTGAATTCAGCT+GGG - chr8.4:36819052-36819071 MS.gene038527:intron 45.0%
ATGCAACCTGGAAGACATCA+TGG - chr8.4:36820702-36820721 MS.gene038527:CDS 45.0%
ATTACTCCAAACGACGGTGT+TGG + chr8.4:36819716-36819735 None:intergenic 45.0%
CAAAATCCAACACCGTCGTT+TGG - chr8.4:36819707-36819726 MS.gene038527:CDS 45.0%
CTCAAACAGGACTGTGTGTT+AGG - chr8.4:36821609-36821628 MS.gene038527:CDS 45.0%
GGAAAGAGATAACAAACCCG+TGG + chr8.4:36821329-36821348 None:intergenic 45.0%
GGAACAGGCTGAAGAAATCA+AGG - chr8.4:36819583-36819602 MS.gene038527:CDS 45.0%
GGAGCTATCATCGTTCCAAA+CGG - chr8.4:36820376-36820395 MS.gene038527:CDS 45.0%
GGTGACGATGAATGCGATAT+TGG - chr8.4:36819653-36819672 MS.gene038527:CDS 45.0%
GTGCCAACATATTCACCGTT+TGG + chr8.4:36820394-36820413 None:intergenic 45.0%
TACTTATGGCCAAACACGGT+TGG - chr8.4:36819811-36819830 MS.gene038527:CDS 45.0%
TCAACTGAGGATCTGACTTC+CGG - chr8.4:36819206-36819225 MS.gene038527:intron 45.0%
TGAAGAAAACAGGCTCCCTT+GGG + chr8.4:36819403-36819422 None:intergenic 45.0%
TGAGGAGATTGATGAGCACA+TGG - chr8.4:36819472-36819491 MS.gene038527:CDS 45.0%
TGATGTTTGTCTGTCCCCAA+GGG - chr8.4:36819385-36819404 MS.gene038527:CDS 45.0%
TGTTCGCTAGCAAACTATCC+CGG - chr8.4:36821079-36821098 MS.gene038527:CDS 45.0%
TTCTATCAGCTACGGCACAA+AGG - chr8.4:36819874-36819893 MS.gene038527:CDS 45.0%
TTCTTCAATGGGATCCAGGA+GGG - chr8.4:36821115-36821134 MS.gene038527:CDS 45.0%
TTGAAGAAAACAGGCTCCCT+TGG + chr8.4:36819404-36819423 None:intergenic 45.0%
TTGGAGGGTTCTATCAGCTA+CGG - chr8.4:36819866-36819885 MS.gene038527:CDS 45.0%
TTGGCGTGTAAGTTCTCATG+TGG + chr8.4:36820790-36820809 None:intergenic 45.0%
TTTCTTCAATGGGATCCAGG+AGG - chr8.4:36821114-36821133 MS.gene038527:CDS 45.0%
TTTGGCTGTTCCACAGATGT+CGG + chr8.4:36818861-36818880 None:intergenic 45.0%
! AAACTGCATTGATGCACGCT+TGG + chr8.4:36820899-36820918 None:intergenic 45.0%
! AAGAAATGTAGGAGCATGCC+GGG + chr8.4:36821100-36821119 None:intergenic 45.0%
! AAGAGGAGATCGACTTTGCT+CGG - chr8.4:36818820-36818839 MS.gene038527:intron 45.0%
! GAAGAAATGTAGGAGCATGC+CGG + chr8.4:36821101-36821120 None:intergenic 45.0%
! GCAGCTTCTTCAAGTTCAGA+TGG + chr8.4:36820298-36820317 None:intergenic 45.0%
!! CTCTCAATTCTGGTGGATTC+CGG - chr8.4:36821417-36821436 MS.gene038527:CDS 45.0%
!!! CCAATATCAGGTGGTTTTGG+CGG + chr8.4:36820838-36820857 None:intergenic 45.0%
ACCTACAAAGCCAAAGCCCT+CGG - chr8.4:36821029-36821048 MS.gene038527:CDS 50.0%
ATACCCAACCTGACGCAGTA+CGG + chr8.4:36821551-36821570 None:intergenic 50.0%
ATCACCGCCAGAAATTACCG+AGG + chr8.4:36821049-36821068 None:intergenic 50.0%
CAACTGAGGATCTGACTTCC+GGG - chr8.4:36819207-36819226 MS.gene038527:intron 50.0%
CACAGATGTCGGAAACGCTT+CGG + chr8.4:36818850-36818869 None:intergenic 50.0%
CCAAAGCCCTCGGTAATTTC+TGG - chr8.4:36821039-36821058 MS.gene038527:CDS 50.0%
CCAGAAATTACCGAGGGCTT+TGG + chr8.4:36821042-36821061 None:intergenic 50.0%
CCGCCAAAACCACCTGATAT+TGG - chr8.4:36820835-36820854 MS.gene038527:CDS 50.0%
CTTCCAGGTTGCATTTGTCC+TGG + chr8.4:36820696-36820715 None:intergenic 50.0%
GAAGAAAACAGGCTCCCTTG+GGG + chr8.4:36819402-36819421 None:intergenic 50.0%
GAGGGTGATCTCTTGTTCGT+TGG - chr8.4:36821133-36821152 MS.gene038527:CDS 50.0%
GCGTGTAAGTTCTCATGTGG+TGG + chr8.4:36820787-36820806 None:intergenic 50.0%
GGTGATCTCTTGTTCGTTGG+AGG - chr8.4:36821136-36821155 MS.gene038527:CDS 50.0%
GTACCAGGACAAATGCAACC+TGG - chr8.4:36820690-36820709 MS.gene038527:CDS 50.0%
GTGATGTTTGTCTGTCCCCA+AGG - chr8.4:36819384-36819403 MS.gene038527:CDS 50.0%
TCACCGCCAGAAATTACCGA+GGG + chr8.4:36821048-36821067 None:intergenic 50.0%
TGAGGTAGCGATGTCGTTGA+CGG - chr8.4:36818986-36819005 MS.gene038527:intron 50.0%
TGGTGTGTGGGCCTTACAAT+AGG - chr8.4:36819915-36819934 MS.gene038527:CDS 50.0%
TTGGCGGAGGTTTAATCAGC+CGG + chr8.4:36820822-36820841 None:intergenic 50.0%
! AAGCCCTCGGTAATTTCTGG+CGG - chr8.4:36821042-36821061 MS.gene038527:CDS 50.0%
!! TGTTTCGAGTTTAGCTGGGG+TGG - chr8.4:36821642-36821661 MS.gene038527:CDS 50.0%
!!! ATATCAGGTGGTTTTGGCGG+AGG + chr8.4:36820835-36820854 None:intergenic 50.0%
ACTCGGGTCGAGTTCTTCAG+AGG - chr8.4:36821187-36821206 MS.gene038527:CDS 55.0%
CCTGTAGCAACACGTCGATC+CGG + chr8.4:36820262-36820281 None:intergenic 55.0%
CGAACAAGAGATCACCCTCC+TGG + chr8.4:36821132-36821151 None:intergenic 55.0%
CGAGTTTAGCTGGGGTGGTA+TGG - chr8.4:36821647-36821666 MS.gene038527:CDS 55.0%
CTTCCGTACTGCGTCAGGTT+GGG - chr8.4:36821545-36821564 MS.gene038527:CDS 55.0%
GACATCGCTACCTCATCGTC+CGG + chr8.4:36818981-36819000 None:intergenic 55.0%
GGACCTTGAGGTTGAGACAC+CGG - chr8.4:36818959-36818978 MS.gene038527:intron 55.0%
GGTTGGGATAAGCGACGTCT+TGG - chr8.4:36820355-36820374 MS.gene038527:CDS 55.0%
GTCGAGTTCTTCAGAGGTGG+AGG - chr8.4:36821193-36821212 MS.gene038527:CDS 55.0%
TGGAGGAGACTGATGCAGGA+GGG - chr8.4:36821210-36821229 MS.gene038527:CDS 55.0%
TTCTTCGGCAGCAGCAGTGA+TGG + chr8.4:36820235-36820254 None:intergenic 55.0%
! CGAAGCGTTTCCGACATCTG+TGG - chr8.4:36818848-36818867 MS.gene038527:intron 55.0%
! CTGCATTGATGCACGCTTGG+TGG + chr8.4:36820896-36820915 None:intergenic 55.0%
! TGACGCAGTACGGAAGCGTA+GGG + chr8.4:36821541-36821560 None:intergenic 55.0%
!! ACCGAGGGCTTTGGCTTTGT+AGG + chr8.4:36821033-36821052 None:intergenic 55.0%
AGCTGAAGTCCTGGGCTTCC+CGG + chr8.4:36819228-36819247 None:intergenic 60.0%
ATCTGACTTCCGGGAAGCCC+AGG - chr8.4:36819216-36819235 MS.gene038527:intron 60.0%
CGGGTCGAGTTCTTCAGAGG+TGG - chr8.4:36821190-36821209 MS.gene038527:CDS 60.0%
CGTCCGGTGTCTCAACCTCA+AGG + chr8.4:36818965-36818984 None:intergenic 60.0%
CGTCGATCCGGTGGCTTCTT+CGG + chr8.4:36820250-36820269 None:intergenic 60.0%
CTACGCTTCCGTACTGCGTC+AGG - chr8.4:36821540-36821559 MS.gene038527:CDS 60.0%
GAGGTGGAGGAGACTGATGC+AGG - chr8.4:36821206-36821225 MS.gene038527:CDS 60.0%
GCTTCCGTACTGCGTCAGGT+TGG - chr8.4:36821544-36821563 MS.gene038527:CDS 60.0%
GGTTGAGACACCGGACGATG+AGG - chr8.4:36818968-36818987 MS.gene038527:intron 60.0%
GTAGCAACACGTCGATCCGG+TGG + chr8.4:36820259-36820278 None:intergenic 60.0%
GTGGAGGAGACTGATGCAGG+AGG - chr8.4:36821209-36821228 MS.gene038527:CDS 60.0%
! CCGGATCGACGTGTTGCTAC+AGG - chr8.4:36820259-36820278 MS.gene038527:CDS 60.0%
! CTGACGCAGTACGGAAGCGT+AGG + chr8.4:36821542-36821561 None:intergenic 60.0%
GCTGCTGCCGAAGAAGCCAC+CGG - chr8.4:36820240-36820259 MS.gene038527:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 36818753 36821680 36818753 ID=MS.gene038527
chr8.4 mRNA 36818753 36821680 36818753 ID=MS.gene038527.t1;Parent=MS.gene038527
chr8.4 exon 36819383 36821680 36819383 ID=MS.gene038527.t1.exon1;Parent=MS.gene038527.t1
chr8.4 CDS 36819383 36821680 36819383 ID=cds.MS.gene038527.t1;Parent=MS.gene038527.t1
chr8.4 exon 36818753 36818815 36818753 ID=MS.gene038527.t1.exon2;Parent=MS.gene038527.t1
chr8.4 CDS 36818753 36818815 36818753 ID=cds.MS.gene038527.t1;Parent=MS.gene038527.t1
Gene Sequence

>MS.gene038527

ATGGTTAAAGATATCGCTAGACATTCTGTTTTGATAACATATATTCTAAGAATTCTTGCGCTATTGAAAGAGGAGATCGACTTTGCTCGGACGACCGAAGCGTTTCCGACATCTGTGGAACAGCCAAATCTGAAGAATACGTTTTCGCCACAACGAGAACCACTGAATAACAAGGTTGAGACACAGATTGTTGAAGCCAAAATATTGGACCTTGAGGTTGAGACACCGGACGATGAGGTAGCGATGTCGTTGACGGAGAACGTAGAAAGTTACGTTGTGTTGACGAAAGAAAGAAGAAAAGTCGTGTCTGAATTCAGCTGGGATAACAATTCACGGAAAATTGAAGTTCCCATATCAGATGATGACGGAATAAGTATACCAATTCACGGAAAAGATGATACCAAGGAAGACACAAGAATTCCATGTGAATATATGGAGCTTTGCAGTGTTGATTCAACTGAGGATCTGACTTCCGGGAAGCCCAGGACTTCAGCTTATGTACTTGACCCATTATTGTCAGAACCACGAGTACAAGTAGAAAGCAAAGCCATTTGTGCTATTAAAACTGCAGAAGCAACAATGAAAAGTGGAAATGGTTTGACAGAACTTGACTGGCAGCAGATTGAAAGAAGTGATGTTTGTCTGTCCCCAAGGGAGCCTGTTTTCTTCAATGATGTTCCTACATTATCAAATATAACAGAAGAACCTGTTGTTAGTATTGAGGAGATTGATGAGCACATGGTTTCACTCTATGATGAAGCAAATATGAATGATGAACCACTGAATGAAGAACCAGAAGAAAAAGAAACTGAAGAGTTTGAAGTGATTGAGGAACAGGCTGAAGAAATCAAGGAGGAAGAATGTGTTGAAAAGGAACATAAAGAATTAAGAACTAAACTTGGTGACGATGAATGCGATATTGGTCATAGCATTATTGACAACTTATCATTAAGACAAAATCCAACACCGTCGTTTGGAGTAATTGACTCTGTTGACTTGGAAAATGGATATAATCAGATATTTCAATCTGCTATGCGGCAACAATCTGCTTGCTCATCTACTTATGGCCAAACACGGTTGGAATCATCAAAGACTTCTTTGGATAAAGGCAAATTGGAGGGTTCTATCAGCTACGGCACAAAGGCACTTTCAAAGTTAGTGTTGGTGTGTGGGCCTTACAATAGGATGACAACAGAAGCTTATAGTCTCTTGGTTGTTGGGCTATACCATACCAGTGATTTCAATCATGCAACCATCTATCAGCTAAAATCACTAAATATAAAGAAAAGATTGCAACACACAAATGTATTGCTTTTCATAGGAACCATATATTCTCAGGAAAGGCGTGCCATAGTCACAAAGTTCTTATTTAGGGGAAGCCTTTTCAGGACACTTCACAAGAACAATCAAACATTAGACATCAAAAAGCATCTTAGAATGGTTCTTGATGTTGCTAGAGGAATAACTTATCTTCAATCGCCATCACTGCTGCTGCCGAAGAAGCCACCGGATCGACGTGTTGCTACAGGTGAAAATGTTGAACCATCTGAACTTGAAGAAGCTGCCATGAGGAGTAGCATAAATCAACAAATTGTAGTTGTTGGTTGGGATAAGCGACGTCTTGGAGCTATCATCGTTCCAAACGGTGAATATGTTGGCACATCCAATGAATTCATCAAGTCCTTAAAGCCAAATTGTAGTCCAAATATAGTCACTGATGGAAATGCATTGCCCATAAAAGAAACTTTGCTTTTGGCTTCTAGTAATTATGAAATTGGAACTAAATGGGGTAAAGAGGTTGGATTTATGTATGGTACTGTATGTGAAGGTGTTATATATGAAAGAGGTTTCATTGGTATTTATCATGACTGGTGTGAAGGCTTTTCTACATACTCAAGGACGTATGATCTTATTCATACTAATGGTTTATTTAGTTTGTACCAGGACAAATGCAACCTGGAAGACATCATGGATAATCATGTCATGGGTGCAATGTATAGATTTCCAAATGCACATGAACATATAGCTCAACCACCACATGAGAACTTACACGCCAAATTTAATTACCGGCTGATTAAACCTCCGCCAAAACCACCTGATATTGGTAAATTTTTATCACTTAATTTGTATCTGATAAATACCACCAAGCGTGCATCAATGCAGTTTTCAGATGAGTATTCGTTTATAGTGCTACAACGAGTTGCTGAAGCCACAAAACAAGCTATCAAACGGAAAATTGTGCAAGCCGCATCTGTTGATTTTCAGTCACGGAGTCTACCTACAAAGCCAAAGCCCTCGGTAATTTCTGGCGGTGATACTGCAAACTTGTTCGCTAGCAAACTATCCCGGCATGCTCCTACATTTCTTCAATGGGATCCAGGAGGGTGATCTCTTGTTCGTTGGAGGAGTCAACATGCTCTTTATCAAAATGAGAACTCGGGTCGAGTTCTTCAGAGGTGGAGGAGACTGATGCAGGAGGGTTTTATTAGTAATTTAGTAGTATTTATTATTTAGCCAAATCTTAGTAATTTTCTATAACTCTCTATTATTATTACGTTTTATTTTATTTTGGCCCACGGGTTTGTTATCTCTTTCCCTATATAAGCAGAACTTATGTAGCCTAAGAGACACACTTTTTGATTCAATAAAATTATTCGAGATTTTCTCTCAATTCTGGTGGATTCCGGAAACCCTTATTTTGATAGGTTTGTCGCTTGCAACCCTATTTTCACAAGTTTGTCGCTTGCAAACTTGTTCTTTTCTATCTAGGTTTAGCGCTTGCTAACCCTACGCTTCCGTACTGCGTCAGGTTGGGTATAAGAATAATCTCTTAGGTAAGAGTTCATGCAAGAATATCTCAAACAGGACTGTGTGTTAGGAGGTTTACATTGTTTCGAGTTTAGCTGGGGTGGTATGGTGAATGCATAA
Protein sequence

>MS.gene038527.t1

MVKDIARHSVLITYILRILALLKEEIDFARTTEAFPTSVEQPNLKNTFSPQREPLNNKVETQIVEAKILDLEVETPDDEVAMSLTENVESYVVLTKERRKVVSEFSWDNNSRKIEVPISDDDGISIPIHGKDDTKEDTRIPCEYMELCSVDSTEDLTSGKPRTSAYVLDPLLSEPRVQVESKAICAIKTAEATMKSGNGLTELDWQQIERSDVCLSPREPVFFNDVPTLSNITEEPVVSIEEIDEHMVSLYDEANMNDEPLNEEPEEKETEEFEVIEEQAEEIKEEECVEKEHKELRTKLGDDECDIGHSIIDNLSLRQNPTPSFGVIDSVDLENGYNQIFQSAMRQQSACSSTYGQTRLESSKTSLDKGKLEGSISYGTKALSKLVLVCGPYNRMTTEAYSLLVVGLYHTSDFNHATIYQLKSLNIKKRLQHTNVLLFIGTIYSQERRAIVTKFLFRGSLFRTLHKNNQTLDIKKHLRMVLDVARGITYLQSPSLLLPKKPPDRRVATGENVEPSELEEAAMRSSINQQIVVVGWDKRRLGAIIVPNGEYVGTSNEFIKSLKPNCSPNIVTDGNALPIKETLLLASSNYEIGTKWGKEVGFMYGTVCEGVIYERGFIGIYHDWCEGFSTYSRTYDLIHTNGLFSLYQDKCNLEDIMDNHVMGAMYRFPNAHEHIAQPPHENLHAKFNYRLIKPPPKPPDIGKFLSLNLYLINTTKRASMQFSDEYSFIVLQRVAEATKQAIKRKIVQAASVDFQSRSLPTKPKPSDCVLGGLHCFEFSWGGMVNA