Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038570.t1 | RHN76102.1 | 89 | 91 | 10 | 0 | 5 | 95 | 264 | 354 | 1.90E-35 | 159.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038570.t1 | Q9XE58 | 53.4 | 88 | 39 | 1 | 8 | 93 | 362 | 449 | 1.4e-18 | 94.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038570.t1 | G7IRV1 | 89.0 | 91 | 10 | 0 | 5 | 95 | 336 | 426 | 1.4e-35 | 159.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene038570.t1 | TF | GRAS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038570.t1 | MTR_2g097410 | 89.011 | 91 | 10 | 0 | 5 | 95 | 336 | 426 | 2.81e-48 | 166 |
| MS.gene038570.t1 | MTR_4g064150 | 60.494 | 81 | 32 | 0 | 19 | 99 | 340 | 420 | 1.12e-28 | 111 |
| MS.gene038570.t1 | MTR_3g087300 | 89.091 | 55 | 6 | 0 | 5 | 59 | 154 | 208 | 1.25e-26 | 100 |
| MS.gene038570.t1 | MTR_4g064160 | 58.824 | 85 | 35 | 0 | 9 | 93 | 285 | 369 | 5.10e-26 | 103 |
| MS.gene038570.t1 | MTR_4g064120 | 59.494 | 79 | 32 | 0 | 21 | 99 | 235 | 313 | 5.94e-26 | 103 |
| MS.gene038570.t1 | MTR_7g062120 | 47.573 | 103 | 44 | 1 | 22 | 124 | 354 | 446 | 4.37e-24 | 98.6 |
| MS.gene038570.t1 | MTR_4g064180 | 61.429 | 70 | 27 | 0 | 21 | 90 | 235 | 304 | 8.86e-24 | 97.4 |
| MS.gene038570.t1 | MTR_2g097390 | 57.143 | 70 | 30 | 0 | 25 | 94 | 260 | 329 | 1.06e-22 | 94.4 |
| MS.gene038570.t1 | MTR_2g097350 | 56.522 | 69 | 30 | 0 | 26 | 94 | 260 | 328 | 3.93e-22 | 92.8 |
| MS.gene038570.t1 | MTR_2g097310 | 58.824 | 68 | 25 | 1 | 30 | 94 | 259 | 326 | 5.31e-21 | 89.7 |
| MS.gene038570.t1 | MTR_2g097463 | 65.517 | 58 | 20 | 0 | 34 | 91 | 284 | 341 | 1.69e-18 | 82.4 |
| MS.gene038570.t1 | MTR_4g064200 | 63.636 | 55 | 20 | 0 | 35 | 89 | 279 | 333 | 4.62e-17 | 78.2 |
| MS.gene038570.t1 | MTR_2g097467 | 51.282 | 78 | 36 | 1 | 16 | 91 | 263 | 340 | 8.63e-17 | 77.4 |
| MS.gene038570.t1 | MTR_2g097473 | 61.404 | 57 | 22 | 0 | 35 | 91 | 284 | 340 | 2.37e-16 | 76.3 |
| MS.gene038570.t1 | MTR_2g097380 | 55.556 | 45 | 20 | 0 | 45 | 89 | 229 | 273 | 2.63e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038570.t1 | AT1G07530 | 53.409 | 88 | 39 | 1 | 8 | 93 | 362 | 449 | 3.07e-24 | 99.0 |
| MS.gene038570.t1 | AT2G37650 | 42.017 | 119 | 57 | 2 | 28 | 144 | 323 | 431 | 1.97e-20 | 87.8 |
| MS.gene038570.t1 | AT2G37650 | 42.017 | 119 | 57 | 2 | 28 | 144 | 335 | 443 | 1.99e-20 | 87.8 |
| MS.gene038570.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 281 | 371 | 2.67e-20 | 87.4 |
| MS.gene038570.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 280 | 370 | 2.86e-20 | 87.4 |
| MS.gene038570.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 116 | 206 | 3.66e-20 | 87.0 |
| MS.gene038570.t1 | AT1G07520 | 51.648 | 91 | 42 | 1 | 4 | 92 | 116 | 206 | 3.66e-20 | 87.0 |
| MS.gene038570.t1 | AT2G29060 | 52.703 | 74 | 35 | 0 | 20 | 93 | 264 | 337 | 1.69e-19 | 85.1 |
| MS.gene038570.t1 | AT2G29060 | 52.703 | 74 | 35 | 0 | 20 | 93 | 301 | 374 | 1.71e-19 | 85.1 |
| MS.gene038570.t1 | AT4G39630 | 49.398 | 83 | 38 | 2 | 94 | 172 | 147 | 229 | 2.55e-17 | 76.6 |
| MS.gene038570.t1 | AT4G39630 | 49.383 | 81 | 37 | 2 | 96 | 172 | 136 | 216 | 4.17e-17 | 75.9 |
| MS.gene038570.t1 | AT2G29065 | 61.017 | 59 | 23 | 0 | 34 | 92 | 246 | 304 | 4.50e-16 | 75.5 |
| MS.gene038570.t1 | AT3G46600 | 40.708 | 113 | 56 | 3 | 6 | 111 | 149 | 257 | 3.37e-15 | 72.8 |
| MS.gene038570.t1 | AT3G46600 | 41.346 | 104 | 51 | 2 | 6 | 102 | 181 | 281 | 3.87e-15 | 72.8 |
| MS.gene038570.t1 | AT5G59450 | 49.275 | 69 | 35 | 0 | 23 | 91 | 210 | 278 | 6.57e-15 | 72.0 |
| MS.gene038570.t1 | AT3G46600 | 46.575 | 73 | 32 | 1 | 37 | 102 | 1 | 73 | 1.17e-12 | 65.5 |
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTATTTAAATTTCTACA+TGG | + | 56881:10474-10493 | MS.gene038570:intron | 15.0% |
| !! | TTAATTAATCATACCATTTA+TGG | - | 56881:11247-11266 | None:intergenic | 15.0% |
| !! | AGATTATCCATGAATTTAAA+AGG | + | 56881:10875-10894 | MS.gene038570:intron | 20.0% |
| !! | TGATTTAATGTCTATTGTAT+AGG | + | 56881:10918-10937 | MS.gene038570:intron | 20.0% |
| !!! | AACATTTCCTTTTAAATTCA+TGG | - | 56881:10885-10904 | None:intergenic | 20.0% |
| !!! | TTACTTTTTGTGACAAAAAT+AGG | + | 56881:10787-10806 | MS.gene038570:intron | 20.0% |
| ! | AAGCATATTTATATACCTCA+TGG | + | 56881:10655-10674 | MS.gene038570:intron | 25.0% |
| ! | AGTTCATTTAAATGAAATGC+AGG | - | 56881:11021-11040 | None:intergenic | 25.0% |
| ! | GTACAGAAATTTGAATTCAT+TGG | + | 56881:9911-9930 | MS.gene038570:intron | 25.0% |
| ! | GTCTTATAAGCTAACTTTAT+TGG | + | 56881:10412-10431 | MS.gene038570:intron | 25.0% |
| ! | TATGTAATATTTAAGCCATG+AGG | - | 56881:10673-10692 | None:intergenic | 25.0% |
| !! | TTTTCTTTTTGTAAGTTGCA+TGG | - | 56881:9937-9956 | None:intergenic | 25.0% |
| !!! | GATATGTTTTTGAGTGTAAA+AGG | + | 56881:11043-11062 | MS.gene038570:intron | 25.0% |
| !!! | TTTGTGATTTTGATTGCAAA+AGG | + | 56881:10317-10336 | MS.gene038570:intron | 25.0% |
| AAAATAGGTTGTGAAACATG+AGG | + | 56881:10802-10821 | MS.gene038570:intron | 30.0% | |
| AAATAGGTTGTGAAACATGA+GGG | + | 56881:10803-10822 | MS.gene038570:intron | 30.0% | |
| AATAACATGGAAGCCATAAA+TGG | + | 56881:11231-11250 | MS.gene038570:CDS | 30.0% | |
| AATGTCTATTGTATAGGCTT+TGG | + | 56881:10924-10943 | MS.gene038570:intron | 30.0% | |
| ATGAATTGTTGAAGCAGATT+AGG | + | 56881:10139-10158 | MS.gene038570:CDS | 30.0% | |
| CATTTAAATGAAATGCAGGA+AGG | - | 56881:11017-11036 | None:intergenic | 30.0% | |
| TGTTTACAAGGACAATAACA+TGG | + | 56881:11218-11237 | MS.gene038570:CDS | 30.0% | |
| ! | AATCAGCACTAAACATCTTT+TGG | - | 56881:10278-10297 | None:intergenic | 30.0% |
| !! | CAGTTACTTTCATTTTGACA+GGG | + | 56881:10519-10538 | MS.gene038570:intron | 30.0% |
| !! | TCAGTTACTTTCATTTTGAC+AGG | + | 56881:10518-10537 | MS.gene038570:intron | 30.0% |
| !! | TGTTGATAATCTGTGTTTCA+AGG | + | 56881:10559-10578 | MS.gene038570:CDS | 30.0% |
| ACCTTTCATCATCATAGAGA+TGG | - | 56881:10382-10401 | None:intergenic | 35.0% | |
| ATTGTCCTTGTAAACACCTA+GGG | - | 56881:11214-11233 | None:intergenic | 35.0% | |
| GATCATGTTACAGAAACGAA+CGG | - | 56881:10854-10873 | None:intergenic | 35.0% | |
| GCTAACATCTAAGTTTCAGA+TGG | - | 56881:10955-10974 | None:intergenic | 35.0% | |
| GTGTAAAAGGTTGTCACAAA+TGG | + | 56881:11056-11075 | MS.gene038570:intron | 35.0% | |
| TATTGTCCTTGTAAACACCT+AGG | - | 56881:11215-11234 | None:intergenic | 35.0% | |
| ! | TCCATCTCTATGATGATGAA+AGG | + | 56881:10378-10397 | MS.gene038570:intron | 35.0% |
| !! | TAGTTAGCGTTGATAATCGT+AGG | + | 56881:10759-10778 | MS.gene038570:intron | 35.0% |
| !!! | AGTGCTGATTTTGATTGTGT+AGG | + | 56881:10288-10307 | MS.gene038570:intron | 35.0% |
| ATACCTTTGGATCACTTGCA+AGG | + | 56881:10623-10642 | MS.gene038570:intron | 40.0% | |
| CTGTGTCTGCTAATGACAAT+AGG | + | 56881:10109-10128 | MS.gene038570:CDS | 40.0% | |
| GCTGTTAACTCTTCAGTTGA+AGG | - | 56881:11082-11101 | None:intergenic | 40.0% | |
| TAGATGTTAGCGATGCAAGA+AGG | + | 56881:10964-10983 | MS.gene038570:intron | 40.0% | |
| TATCCTTGCAAGTGATCCAA+AGG | - | 56881:10629-10648 | None:intergenic | 40.0% | |
| TGAACAAAAGGATGGATCAG+TGG | + | 56881:9972-9991 | MS.gene038570:intron | 40.0% | |
| TTGTCCTTGTAAACACCTAG+GGG | - | 56881:11213-11232 | None:intergenic | 40.0% | |
| TTGTGCACACAGAACCAAAA+GGG | - | 56881:10088-10107 | None:intergenic | 40.0% | |
| TTGTGTGCTGAACAAAAGGA+TGG | + | 56881:9964-9983 | MS.gene038570:intron | 40.0% | |
| ! | AGTAGTCAGAGGTTTTCTGT+TGG | + | 56881:11123-11142 | MS.gene038570:CDS | 40.0% |
| ! | GTAGTCAGAGGTTTTCTGTT+GGG | + | 56881:11124-11143 | MS.gene038570:CDS | 40.0% |
| ! | GTCTATTGTATAGGCTTTGG+AGG | + | 56881:10927-10946 | MS.gene038570:intron | 40.0% |
| ! | TCCTTTTGTTCAGCACACAA+CGG | - | 56881:9964-9983 | None:intergenic | 40.0% |
| !! | ACACAGGTTGGTAAACTTGA+TGG | + | 56881:10006-10025 | MS.gene038570:CDS | 40.0% |
| !! | AGGTTGGTAAACTTGATGGA+GGG | + | 56881:10010-10029 | MS.gene038570:CDS | 40.0% |
| AAGGCGTGTTCGAAGAAACA+AGG | + | 56881:10033-10052 | MS.gene038570:CDS | 45.0% | |
| ACAAAAGGATGGATCAGTGG+TGG | + | 56881:9975-9994 | MS.gene038570:intron | 45.0% | |
| AGAGGGATGTTGTGGATTTG+AGG | + | 56881:10061-10080 | MS.gene038570:CDS | 45.0% | |
| AGGCGTGTTCGAAGAAACAA+GGG | + | 56881:10034-10053 | MS.gene038570:CDS | 45.0% | |
| AGGTGATGCATCACAGAGAA+CGG | + | 56881:10179-10198 | MS.gene038570:CDS | 45.0% | |
| ATCACCCCTAGGTGTTTACA+AGG | + | 56881:11206-11225 | MS.gene038570:CDS | 45.0% | |
| CTGCTACACTCACATACCTT+TGG | + | 56881:10610-10629 | MS.gene038570:intron | 45.0% | |
| CTTGTGCACACAGAACCAAA+AGG | - | 56881:10089-10108 | None:intergenic | 45.0% | |
| GAAGAAACAAGGGAGGAAGA+GGG | + | 56881:10044-10063 | MS.gene038570:CDS | 45.0% | |
| GAAGGAGAGATACTTACACC+AGG | - | 56881:10999-11018 | None:intergenic | 45.0% | |
| GAGATGCTTCTTTCTGTCCA+TGG | + | 56881:11183-11202 | MS.gene038570:CDS | 45.0% | |
| GATGACACCCAAAACACTTC+GGG | + | 56881:11146-11165 | MS.gene038570:CDS | 45.0% | |
| GGATGACACCCAAAACACTT+CGG | + | 56881:11145-11164 | MS.gene038570:CDS | 45.0% | |
| TGGTAAACTTGATGGAGGGA+AGG | + | 56881:10014-10033 | MS.gene038570:CDS | 45.0% | |
| ! | ACAGGGAGAACAAGCTTTGT+TGG | + | 56881:10536-10555 | MS.gene038570:intron | 45.0% |
| ! | ACTTTATTGGCTGCAAGCTC+AGG | + | 56881:10425-10444 | MS.gene038570:intron | 45.0% |
| ! | AGTTACTTTCTTGCCTCAGG+GGG | + | 56881:10341-10360 | MS.gene038570:intron | 45.0% |
| ! | CAAAGGTATGTGAGTGTAGC+AGG | - | 56881:10612-10631 | None:intergenic | 45.0% |
| ! | CAGCTGAATATGCGTTTTGC+AGG | + | 56881:11098-11117 | MS.gene038570:intron | 45.0% |
| ! | CAGTTACTTTCTTGCCTCAG+GGG | + | 56881:10340-10359 | MS.gene038570:intron | 45.0% |
| ! | CTTTATTGGCTGCAAGCTCA+GGG | + | 56881:10426-10445 | MS.gene038570:intron | 45.0% |
| ! | GCAGTTACTTTCTTGCCTCA+GGG | + | 56881:10339-10358 | MS.gene038570:intron | 45.0% |
| ! | TGTGGATTTGAGGACCCTTT+TGG | + | 56881:10071-10090 | MS.gene038570:CDS | 45.0% |
| ! | TTTTGCAGGTGAGTAGTCAG+AGG | + | 56881:11112-11131 | MS.gene038570:intron | 45.0% |
| !! | ACAGAAAGAAGCATCTCTCC+AGG | - | 56881:11180-11199 | None:intergenic | 45.0% |
| !! | AGGAAGCATTCTTGTCCTTC+AGG | + | 56881:10159-10178 | MS.gene038570:CDS | 45.0% |
| !! | CAGGTTGGTAAACTTGATGG+AGG | + | 56881:10009-10028 | MS.gene038570:CDS | 45.0% |
| !! | TTGGGAACCCGAAGTGTTTT+GGG | - | 56881:11156-11175 | None:intergenic | 45.0% |
| AAACACCTAGGGGTGATCCA+TGG | - | 56881:11203-11222 | None:intergenic | 50.0% | |
| CGAAGAAACAAGGGAGGAAG+AGG | + | 56881:10043-10062 | MS.gene038570:CDS | 50.0% | |
| CGTGTTCGAAGAAACAAGGG+AGG | + | 56881:10037-10056 | MS.gene038570:CDS | 50.0% | |
| CTCTGTGATGCATCACCTGA+AGG | - | 56881:10177-10196 | None:intergenic | 50.0% | |
| GCCGTTGTGTGCTGAACAAA+AGG | + | 56881:9960-9979 | MS.gene038570:intron | 50.0% | |
| GTGGAGAGTAGAACACAGGT+TGG | + | 56881:9994-10013 | MS.gene038570:CDS | 50.0% | |
| TAATGCGATTGAAGCGCGCA+TGG | + | 56881:10215-10234 | MS.gene038570:CDS | 50.0% | |
| ! | AGTGGTGGAGAGTAGAACAC+AGG | + | 56881:9990-10009 | MS.gene038570:CDS | 50.0% |
| ! | GGCAGTTACTTTCTTGCCTC+AGG | + | 56881:10338-10357 | MS.gene038570:intron | 50.0% |
| !! | AAGAAGCATCTCTCCAGGCT+TGG | - | 56881:11175-11194 | None:intergenic | 50.0% |
| !! | AGAAGCATCTCTCCAGGCTT+GGG | - | 56881:11174-11193 | None:intergenic | 50.0% |
| !! | CTTGGGAACCCGAAGTGTTT+TGG | - | 56881:11157-11176 | None:intergenic | 50.0% |
| AGGGAGGAAGAGGGATGTTG+TGG | + | 56881:10053-10072 | MS.gene038570:CDS | 55.0% | |
| CATACAATCTCGTCCCCCTG+AGG | - | 56881:10357-10376 | None:intergenic | 55.0% | |
| GCAAGAAGGTCTCTGACTCC+TGG | + | 56881:10978-10997 | MS.gene038570:intron | 55.0% | |
| TCTGTCCATGGATCACCCCT+AGG | + | 56881:11195-11214 | MS.gene038570:CDS | 55.0% | |
| !! | CGCATGGTTGGTGCTGGTAA+CGG | + | 56881:10231-10250 | MS.gene038570:CDS | 55.0% |
| GCGATTGAAGCGCGCATGGT+TGG | + | 56881:10219-10238 | MS.gene038570:CDS | 60.0% | |
| ! | ACACTTCGGGTTCCCAAGCC+TGG | + | 56881:11159-11178 | MS.gene038570:CDS | 60.0% |
| ! | GAAGCGCGCATGGTTGGTGC+TGG | + | 56881:10225-10244 | MS.gene038570:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56881 | gene | 9895 | 11266 | 9895 | ID=MS.gene038570 |
| 56881 | mRNA | 9895 | 11266 | 9895 | ID=MS.gene038570.t1;Parent=MS.gene038570 |
| 56881 | exon | 9895 | 9906 | 9895 | ID=MS.gene038570.t1.exon1;Parent=MS.gene038570.t1 |
| 56881 | CDS | 9895 | 9906 | 9895 | ID=cds.MS.gene038570.t1;Parent=MS.gene038570.t1 |
| 56881 | exon | 9982 | 10252 | 9982 | ID=MS.gene038570.t1.exon2;Parent=MS.gene038570.t1 |
| 56881 | CDS | 9982 | 10252 | 9982 | ID=cds.MS.gene038570.t1;Parent=MS.gene038570.t1 |
| 56881 | exon | 10540 | 10586 | 10540 | ID=MS.gene038570.t1.exon3;Parent=MS.gene038570.t1 |
| 56881 | CDS | 10540 | 10586 | 10540 | ID=cds.MS.gene038570.t1;Parent=MS.gene038570.t1 |
| 56881 | exon | 10809 | 10862 | 10809 | ID=MS.gene038570.t1.exon4;Parent=MS.gene038570.t1 |
| 56881 | CDS | 10809 | 10862 | 10809 | ID=cds.MS.gene038570.t1;Parent=MS.gene038570.t1 |
| 56881 | exon | 11120 | 11266 | 11120 | ID=MS.gene038570.t1.exon5;Parent=MS.gene038570.t1 |
| 56881 | CDS | 11120 | 11266 | 11120 | ID=cds.MS.gene038570.t1;Parent=MS.gene038570.t1 |
| Gene Sequence |
| Protein sequence |
