Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038614.t1 | XP_003628696.1 | 93.8 | 144 | 9 | 0 | 1 | 144 | 1 | 144 | 1.60E-67 | 265.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038614.t1 | F4JZT3 | 56.8 | 118 | 49 | 1 | 10 | 127 | 16 | 131 | 3.0e-27 | 122.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038614.t1 | G7LG58 | 93.8 | 144 | 9 | 0 | 1 | 144 | 1 | 144 | 1.1e-67 | 265.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene038614.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038614.t1 | MTR_8g063580 | 93.750 | 144 | 9 | 0 | 1 | 144 | 1 | 144 | 9.59e-98 | 277 |
| MS.gene038614.t1 | MTR_7g099500 | 68.182 | 132 | 36 | 3 | 1 | 132 | 1 | 126 | 2.68e-54 | 167 |
| MS.gene038614.t1 | MTR_7g098910 | 58.400 | 125 | 49 | 1 | 5 | 129 | 15 | 136 | 2.14e-42 | 137 |
| MS.gene038614.t1 | MTR_6g008860 | 34.259 | 108 | 69 | 1 | 12 | 119 | 33 | 138 | 2.62e-14 | 66.2 |
| MS.gene038614.t1 | MTR_4g071650 | 34.259 | 108 | 69 | 1 | 12 | 119 | 33 | 138 | 2.62e-14 | 66.2 |
| MS.gene038614.t1 | MTR_0022s0450 | 54.098 | 61 | 28 | 0 | 18 | 78 | 40 | 100 | 2.94e-14 | 64.7 |
| MS.gene038614.t1 | MTR_8g075340 | 31.915 | 141 | 73 | 4 | 12 | 131 | 1029 | 1167 | 1.52e-13 | 67.0 |
| MS.gene038614.t1 | MTR_8g106150 | 31.159 | 138 | 67 | 4 | 15 | 132 | 864 | 993 | 4.42e-13 | 65.5 |
| MS.gene038614.t1 | MTR_8g106150 | 31.159 | 138 | 67 | 4 | 15 | 132 | 621 | 750 | 4.68e-13 | 65.5 |
| MS.gene038614.t1 | MTR_7g098860 | 45.122 | 82 | 42 | 2 | 31 | 111 | 8 | 87 | 4.17e-12 | 58.9 |
| MS.gene038614.t1 | MTR_1g087140 | 38.017 | 121 | 55 | 5 | 15 | 122 | 976 | 1089 | 1.27e-11 | 61.6 |
| MS.gene038614.t1 | MTR_5g097410 | 41.791 | 67 | 39 | 0 | 15 | 81 | 1077 | 1143 | 2.93e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038614.t1 | AT5G26594 | 56.780 | 118 | 49 | 1 | 10 | 127 | 16 | 131 | 9.93e-38 | 125 |
| MS.gene038614.t1 | AT3G04280 | 50.000 | 116 | 56 | 2 | 12 | 126 | 22 | 136 | 2.18e-31 | 109 |
| MS.gene038614.t1 | AT3G04280 | 50.000 | 116 | 56 | 2 | 12 | 126 | 22 | 136 | 2.18e-31 | 109 |
| MS.gene038614.t1 | AT3G04280 | 50.000 | 116 | 56 | 2 | 12 | 126 | 22 | 136 | 2.18e-31 | 109 |
| MS.gene038614.t1 | AT2G17820 | 27.329 | 161 | 79 | 3 | 15 | 139 | 1047 | 1205 | 1.55e-12 | 64.3 |
| MS.gene038614.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 937 | 1052 | 5.40e-12 | 62.4 |
| MS.gene038614.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 948 | 1063 | 5.56e-12 | 62.4 |
| MS.gene038614.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
| MS.gene038614.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
| MS.gene038614.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
| MS.gene038614.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
Find 27 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATAGTGATAAAATTTA+TGG | 0.245050 | 8.3:+38689394 | MS.gene038614:CDS |
| TGGTGTATCCTCACACTCTA+TGG | 0.342570 | 8.3:+38689364 | MS.gene038614:CDS |
| TGACCTAATCTTGATGGATA+AGG | 0.347722 | 8.3:+38689081 | MS.gene038614:CDS |
| ACGTCATTTGATGAATTCTT+CGG | 0.351042 | 8.3:-38688979 | None:intergenic |
| CACAACAAGTGCTGTTAACT+TGG | 0.355344 | 8.3:-38688939 | None:intergenic |
| CGTCATTTGATGAATTCTTC+GGG | 0.366651 | 8.3:-38688978 | None:intergenic |
| AACAAAGCAACTAAGGTTGA+TGG | 0.449452 | 8.3:+38689322 | MS.gene038614:CDS |
| GTGATAAAATTTATGGAAGC+AGG | 0.456780 | 8.3:+38689401 | MS.gene038614:CDS |
| AATTCCATTCTTCATGCCGT+TGG | 0.460947 | 8.3:+38689467 | MS.gene038614:CDS |
| TATCCTTATCCATCAAGATT+AGG | 0.462938 | 8.3:-38689084 | None:intergenic |
| TCTTGAGAAACCATTGGATA+AGG | 0.467024 | 8.3:+38689436 | MS.gene038614:CDS |
| GGGATATCTAGCACTATTGT+TGG | 0.469032 | 8.3:+38689344 | MS.gene038614:CDS |
| TATAGGCAACAAAGCAACTA+AGG | 0.474253 | 8.3:+38689315 | MS.gene038614:intron |
| TCATTTCATCTTGTGAGGAA+TGG | 0.476235 | 8.3:-38689513 | None:intergenic |
| CAAATGACGTTGAGTAACTT+AGG | 0.479404 | 8.3:+38688992 | MS.gene038614:CDS |
| ACCTCAGCTCCATTCATCTC+AGG | 0.491878 | 8.3:-38689109 | None:intergenic |
| AAGGATATGCCTGAGATGAA+TGG | 0.499108 | 8.3:+38689100 | MS.gene038614:CDS |
| ACAAAGCAACTAAGGTTGAT+GGG | 0.523464 | 8.3:+38689323 | MS.gene038614:CDS |
| CGAATATCTTGAGAAACCAT+TGG | 0.533361 | 8.3:+38689430 | MS.gene038614:CDS |
| TTTGTTCATTTCATCTTGTG+AGG | 0.533712 | 8.3:-38689518 | None:intergenic |
| CTCCTCTTCCATAGAGTGTG+AGG | 0.555361 | 8.3:-38689372 | None:intergenic |
| ATCCTCACACTCTATGGAAG+AGG | 0.570487 | 8.3:+38689370 | MS.gene038614:CDS |
| CAAGTTAACAGCACTTGTTG+TGG | 0.584291 | 8.3:+38688940 | MS.gene038614:CDS |
| CTCACACTCTATGGAAGAGG+AGG | 0.604784 | 8.3:+38689373 | MS.gene038614:CDS |
| CAAAGCAACTAAGGTTGATG+GGG | 0.617733 | 8.3:+38689324 | MS.gene038614:CDS |
| GCAGTGGAAATTCATCAGCA+TGG | 0.649906 | 8.3:+38689049 | MS.gene038614:CDS |
| GCCTGAGATGAATGGAGCTG+AGG | 0.680215 | 8.3:+38689108 | MS.gene038614:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATGTGTTTCTTATAT+AGG | + | chr8.3:38689298-38689317 | MS.gene038614:intron | 15.0% |
| !!! | TTTTTCTTTTATTTTTCCAA+CGG | - | chr8.3:38689486-38689505 | None:intergenic | 15.0% |
| !! | GGAAATAGTGATAAAATTTA+TGG | + | chr8.3:38689394-38689413 | MS.gene038614:CDS | 20.0% |
| !! | AATTTTTGACCTAATCTTGA+TGG | + | chr8.3:38689075-38689094 | MS.gene038614:CDS | 25.0% |
| AACAACACTTGTGTGAAAAA+CGG | + | chr8.3:38689019-38689038 | MS.gene038614:CDS | 30.0% | |
| ACGTCATTTGATGAATTCTT+CGG | - | chr8.3:38688982-38689001 | None:intergenic | 30.0% | |
| GAGTTCATTCTTGCTTATTT+AGG | + | chr8.3:38689190-38689209 | MS.gene038614:intron | 30.0% | |
| GTGATAAAATTTATGGAAGC+AGG | + | chr8.3:38689401-38689420 | MS.gene038614:CDS | 30.0% | |
| TATCCTTATCCATCAAGATT+AGG | - | chr8.3:38689087-38689106 | None:intergenic | 30.0% | |
| !!! | TTGGATGTAAAGTTTTGAGA+TGG | - | chr8.3:38688923-38688942 | None:intergenic | 30.0% |
| AACAAAGCAACTAAGGTTGA+TGG | + | chr8.3:38689322-38689341 | MS.gene038614:CDS | 35.0% | |
| CAAATGACGTTGAGTAACTT+AGG | + | chr8.3:38688992-38689011 | MS.gene038614:CDS | 35.0% | |
| CGAATATCTTGAGAAACCAT+TGG | + | chr8.3:38689430-38689449 | MS.gene038614:CDS | 35.0% | |
| CGTCATTTGATGAATTCTTC+GGG | - | chr8.3:38688981-38689000 | None:intergenic | 35.0% | |
| TATAGGCAACAAAGCAACTA+AGG | + | chr8.3:38689315-38689334 | MS.gene038614:intron | 35.0% | |
| TCATTTCATCTTGTGAGGAA+TGG | - | chr8.3:38689516-38689535 | None:intergenic | 35.0% | |
| TCTTGAGAAACCATTGGATA+AGG | + | chr8.3:38689436-38689455 | MS.gene038614:CDS | 35.0% | |
| ! | ACAAAGCAACTAAGGTTGAT+GGG | + | chr8.3:38689323-38689342 | MS.gene038614:CDS | 35.0% |
| ! | TGACCTAATCTTGATGGATA+AGG | + | chr8.3:38689081-38689100 | MS.gene038614:CDS | 35.0% |
| ! | TTGAGTTTTGCCTTATCCAA+TGG | - | chr8.3:38689449-38689468 | None:intergenic | 35.0% |
| AAGGATATGCCTGAGATGAA+TGG | + | chr8.3:38689100-38689119 | MS.gene038614:CDS | 40.0% | |
| AATTCCATTCTTCATGCCGT+TGG | + | chr8.3:38689467-38689486 | MS.gene038614:CDS | 40.0% | |
| CATTCTTGCTTATTTAGGCG+TGG | + | chr8.3:38689195-38689214 | MS.gene038614:intron | 40.0% | |
| GAAAAACGGAAAAGAAGCAG+TGG | + | chr8.3:38689033-38689052 | MS.gene038614:CDS | 40.0% | |
| GGGATATCTAGCACTATTGT+TGG | + | chr8.3:38689344-38689363 | MS.gene038614:CDS | 40.0% | |
| ! | CAAGTTAACAGCACTTGTTG+TGG | + | chr8.3:38688940-38688959 | MS.gene038614:CDS | 40.0% |
| ! | CACAACAAGTGCTGTTAACT+TGG | - | chr8.3:38688942-38688961 | None:intergenic | 40.0% |
| ! | TTTTCCAACGGCATGAAGAA+TGG | - | chr8.3:38689474-38689493 | None:intergenic | 40.0% |
| !! | CAAAGCAACTAAGGTTGATG+GGG | + | chr8.3:38689324-38689343 | MS.gene038614:CDS | 40.0% |
| ATCCTCACACTCTATGGAAG+AGG | + | chr8.3:38689370-38689389 | MS.gene038614:CDS | 45.0% | |
| GCAGTGGAAATTCATCAGCA+TGG | + | chr8.3:38689049-38689068 | MS.gene038614:CDS | 45.0% | |
| TGGTGTATCCTCACACTCTA+TGG | + | chr8.3:38689364-38689383 | MS.gene038614:CDS | 45.0% | |
| !! | ATATATAAATGTAAAATTTA+GGG | - | chr8.3:38689154-38689173 | None:intergenic | 5.0% |
| !! | TATATATAAATGTAAAATTT+AGG | - | chr8.3:38689155-38689174 | None:intergenic | 5.0% |
| ACCTCAGCTCCATTCATCTC+AGG | - | chr8.3:38689112-38689131 | None:intergenic | 50.0% | |
| CTCACACTCTATGGAAGAGG+AGG | + | chr8.3:38689373-38689392 | MS.gene038614:CDS | 50.0% | |
| CTCCTCTTCCATAGAGTGTG+AGG | - | chr8.3:38689375-38689394 | None:intergenic | 50.0% | |
| GCCTGAGATGAATGGAGCTG+AGG | + | chr8.3:38689108-38689127 | MS.gene038614:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 38688911 | 38689535 | 38688911 | ID=MS.gene038614 |
| chr8.3 | mRNA | 38688911 | 38689535 | 38688911 | ID=MS.gene038614.t1;Parent=MS.gene038614 |
| chr8.3 | exon | 38688911 | 38689129 | 38688911 | ID=MS.gene038614.t1.exon1;Parent=MS.gene038614.t1 |
| chr8.3 | CDS | 38688911 | 38689129 | 38688911 | ID=cds.MS.gene038614.t1;Parent=MS.gene038614.t1 |
| chr8.3 | exon | 38689320 | 38689535 | 38689320 | ID=MS.gene038614.t1.exon2;Parent=MS.gene038614.t1 |
| chr8.3 | CDS | 38689320 | 38689535 | 38689320 | ID=cds.MS.gene038614.t1;Parent=MS.gene038614.t1 |
| Gene Sequence |
| Protein sequence |
