AlfalfaGEDB | MS.gene03923

M. sativa cultivar XinJiangDaYe / MS.gene03923

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03923.t1	KEH34370.1	93.7	143	9	0	1	143	1	143	2.20E-66	261.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03923.t1	Q9FJK3	46.4	140	69	4	1	138	29	164	2.6e-23	109.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03923.t1	A0A072V8A1	93.7	143	9	0	1	143	1	143	1.6e-66	261.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene03923.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03923.t1	MTR_3g465410	93.706	143	9	0	1	143	1	143	5.10e-97	275
MS.gene03923.t1	MTR_4g063790	90.909	143	13	0	1	143	29	171	3.49e-92	264
MS.gene03923.t1	MTR_3g467080	77.444	133	30	0	1	133	29	161	7.63e-70	207
MS.gene03923.t1	MTR_3g466980	49.306	144	70	2	1	142	29	171	3.64e-42	139
MS.gene03923.t1	MTR_3g031240	47.407	135	71	0	1	135	29	163	2.70e-38	128
MS.gene03923.t1	MTR_2g016210	43.262	141	80	0	1	141	29	169	1.87e-36	125
MS.gene03923.t1	MTR_3g065100	47.244	127	66	1	4	130	32	157	2.24e-36	125
MS.gene03923.t1	MTR_3g031100	42.553	141	81	0	1	141	29	169	3.20e-34	119
MS.gene03923.t1	MTR_5g075380	40.909	132	71	2	1	129	29	156	4.14e-32	112
MS.gene03923.t1	MTR_4g032620	39.416	137	83	0	1	137	29	165	9.18e-32	113
MS.gene03923.t1	MTR_4g032290	39.552	134	81	0	4	137	32	165	1.55e-30	110
MS.gene03923.t1	MTR_2g035580	47.200	125	65	1	2	126	30	153	9.70e-30	105
MS.gene03923.t1	MTR_4g031910	38.806	134	82	0	4	137	32	165	5.23e-29	105
MS.gene03923.t1	MTR_4g032260	35.938	128	82	0	4	131	32	159	7.81e-28	101
MS.gene03923.t1	MTR_8g036130	38.849	139	83	1	1	137	29	167	4.27e-27	101
MS.gene03923.t1	MTR_2g035610	46.512	86	46	0	4	89	32	117	2.86e-25	93.6
MS.gene03923.t1	MTR_5g047580	38.095	126	76	2	1	126	29	152	6.16e-23	88.6
MS.gene03923.t1	MTR_1g077320	36.719	128	79	2	4	131	32	157	1.89e-22	87.4
MS.gene03923.t1	MTR_1g077300	35.385	130	80	2	4	133	32	157	2.16e-22	87.0
MS.gene03923.t1	MTR_4g028720	35.938	128	80	2	4	131	32	157	2.17e-22	87.0
MS.gene03923.t1	MTR_3g466830	36.434	129	81	1	1	129	28	155	5.57e-22	86.3
MS.gene03923.t1	MTR_1g077390	36.641	131	81	2	1	131	29	157	6.75e-22	85.9
MS.gene03923.t1	MTR_7g011950	35.156	128	81	2	4	131	32	157	1.08e-21	85.5
MS.gene03923.t1	MTR_1g084950	34.127	126	81	2	4	129	32	155	1.51e-21	84.7
MS.gene03923.t1	MTR_3g466930	34.921	126	81	1	4	129	2	126	5.04e-20	80.5
MS.gene03923.t1	MTR_1g090783	34.921	126	80	2	1	126	28	151	1.10e-19	80.1
MS.gene03923.t1	MTR_3g466900	33.333	126	83	1	4	129	2	126	2.99e-18	75.9
MS.gene03923.t1	MTR_3g466890	33.594	128	84	1	2	129	29	155	2.49e-17	73.9
MS.gene03923.t1	MTR_1g090710	31.746	126	85	1	1	126	29	153	3.77e-16	70.9
MS.gene03923.t1	MTR_7g055800	37.931	87	39	1	3	89	18	89	1.36e-15	67.8
MS.gene03923.t1	MTR_1g090697	32.812	128	80	4	1	126	29	152	7.41e-15	67.4
MS.gene03923.t1	MTR_5g047560	35.398	113	71	2	14	126	26	136	2.60e-14	65.9
MS.gene03923.t1	MTR_7g055940	38.158	76	32	1	3	78	18	78	9.65e-14	62.8
MS.gene03923.t1	MTR_4g019670	32.727	110	64	2	1	107	32	134	2.71e-12	63.2
MS.gene03923.t1	MTR_7g106510	31.579	114	63	3	1	111	32	133	4.80e-12	62.4
MS.gene03923.t1	MTR_4g028800	50.000	44	22	0	1	44	27	70	1.37e-11	57.0
MS.gene03923.t1	MTR_4g084780	53.704	54	25	0	46	99	1	54	6.50e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03923.t1	AT5G48670	45.255	137	69	4	4	138	32	164	4.31e-31	113
MS.gene03923.t1	AT5G27810	52.809	89	42	0	1	89	1	89	2.32e-29	103
MS.gene03923.t1	AT1G65300	40.000	125	74	1	2	126	29	152	5.63e-23	91.7
MS.gene03923.t1	AT1G65330	39.370	127	76	1	1	127	28	153	1.45e-22	90.5
MS.gene03923.t1	AT3G05860	36.806	144	86	2	1	142	29	169	4.09e-21	85.1
MS.gene03923.t1	AT3G05860	36.806	144	86	2	1	142	29	169	5.69e-21	85.5
MS.gene03923.t1	AT3G05860	36.806	144	86	2	1	142	29	169	7.27e-21	85.5
MS.gene03923.t1	AT5G26650	34.586	133	78	2	1	129	27	154	5.19e-20	84.7
MS.gene03923.t1	AT5G26630	35.537	121	77	1	6	126	34	153	5.59e-20	82.4
MS.gene03923.t1	AT1G31640	37.037	135	71	4	2	130	29	155	2.03e-19	83.6
MS.gene03923.t1	AT5G27960	43.529	85	48	0	1	85	27	111	3.43e-18	79.3
MS.gene03923.t1	AT1G31630	33.333	129	82	3	3	130	30	155	5.46e-17	76.3
MS.gene03923.t1	AT1G22590	37.647	85	53	0	1	85	29	113	1.24e-15	69.7
MS.gene03923.t1	AT2G28700	31.081	148	91	4	1	143	29	170	1.05e-12	64.3
MS.gene03923.t1	AT5G06500	27.907	129	85	3	1	126	29	152	6.28e-12	61.2

Find 34 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGGTCATTTCCATCTTTC+TGG	0.077675	3.2:+55905179	None:intergenic
ATTCAAACCATAGAATTTAA+TGG	0.173791	3.2:-55905141	MS.gene03923:CDS
CGAATTTCCATTAAATTCTA+TGG	0.218755	3.2:+55905134	None:intergenic
GAGGTTTGGCCATCAGATTC+AGG	0.258145	3.2:-55905336	MS.gene03923:CDS
TCTTCTTGAGGCAAAACATT+AGG	0.355934	3.2:-55905239	MS.gene03923:CDS
AATATAATGATCAATGATAT+TGG	0.368342	3.2:+55905161	None:intergenic
ATCAAGAGGAGGTTGGAAAT+GGG	0.388178	3.2:-55905020	MS.gene03923:CDS
GATCAAGAGGAGGTTGGAAA+TGG	0.401299	3.2:-55905021	MS.gene03923:CDS
GAAGTGTGATATCTAGGTTT+AGG	0.426765	3.2:-55905308	MS.gene03923:CDS
GGGTCAGAAGTGTGATATCT+AGG	0.436600	3.2:-55905314	MS.gene03923:CDS
TCTAGGTTTAGGAGTTTGCC+TGG	0.445921	3.2:-55905297	MS.gene03923:CDS
AGAATTTGAAGGAGGTTGAT+CGG	0.478161	3.2:-55905068	MS.gene03923:CDS
ACAACCCTCGAGTAGAGGTT+TGG	0.487846	3.2:-55905350	MS.gene03923:CDS
TCAAGAGGAGGTTGGAAATG+GGG	0.500358	3.2:-55905019	MS.gene03923:CDS
AGGTTTGGCCATCAGATTCA+GGG	0.511068	3.2:-55905335	MS.gene03923:CDS
ACGAGCAGTTGAAGAAGCAA+AGG	0.512962	3.2:-55905209	MS.gene03923:CDS
AAGGGAGGAAACCAGAAAGA+TGG	0.513695	3.2:-55905190	MS.gene03923:CDS
ATGAAAGATCAAGAGGAGGT+TGG	0.519383	3.2:-55905027	MS.gene03923:CDS
GAGGAGGTTGGAAATGGGGT+GGG	0.563227	3.2:-55905015	MS.gene03923:CDS
AGAGGAGGTTGGAAATGGGG+TGG	0.565910	3.2:-55905016	MS.gene03923:CDS
ACATGCTTCTACTCCACATA+GGG	0.576406	3.2:+55905392	None:intergenic
CGAGCAGTTGAAGAAGCAAA+GGG	0.580872	3.2:-55905208	MS.gene03923:CDS
AATGACAATGAAAGATCAAG+AGG	0.583122	3.2:-55905034	MS.gene03923:CDS
TATTGATGAGAATTTGAAGG+AGG	0.592173	3.2:-55905076	MS.gene03923:CDS
AATGAAATTAGCACCCTATG+TGG	0.604835	3.2:-55905405	MS.gene03923:CDS
CACATGCTTCTACTCCACAT+AGG	0.606938	3.2:+55905391	None:intergenic
GCAAAACAACCCTCGAGTAG+AGG	0.609086	3.2:-55905355	MS.gene03923:CDS
CTTCTGACCCCTGAATCTGA+TGG	0.624024	3.2:+55905327	None:intergenic
GATGGCCAAACCTCTACTCG+AGG	0.634480	3.2:+55905345	None:intergenic
GACAATGAAAGATCAAGAGG+AGG	0.653972	3.2:-55905031	MS.gene03923:CDS
GGTTTGGCCATCAGATTCAG+GGG	0.657992	3.2:-55905334	MS.gene03923:CDS
ATGGCCAAACCTCTACTCGA+GGG	0.659257	3.2:+55905346	None:intergenic
GCAGTTGAAGAAGCAAAGGG+AGG	0.693727	3.2:-55905205	MS.gene03923:CDS
TGGATCAGGAGGTCTTCTTG+AGG	0.700337	3.2:-55905251	MS.gene03923:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATAATGATCAATGATAT+TGG	+	chr3.2:55905267-55905286	None:intergenic	15.0%
!!	ATTCAAACCATAGAATTTAA+TGG	-	chr3.2:55905284-55905303	MS.gene03923:CDS	20.0%
!!!	TTTTATTGATGAGAATTTGA+AGG	-	chr3.2:55905346-55905365	MS.gene03923:CDS	20.0%
!	ACATGAACAAAGTAAAAAGA+TGG	-	chr3.2:55905151-55905170	MS.gene03923:CDS	25.0%
!	CGAATTTCCATTAAATTCTA+TGG	+	chr3.2:55905294-55905313	None:intergenic	25.0%
	AATGACAATGAAAGATCAAG+AGG	-	chr3.2:55905391-55905410	MS.gene03923:CDS	30.0%
	TATTGATGAGAATTTGAAGG+AGG	-	chr3.2:55905349-55905368	MS.gene03923:CDS	30.0%
	TGAACAAAGTAAAAAGATGG+TGG	-	chr3.2:55905154-55905173	MS.gene03923:CDS	30.0%
!	TTTTTACTTTGTTCATGTCC+AGG	+	chr3.2:55905149-55905168	None:intergenic	30.0%
	AATGAAATTAGCACCCTATG+TGG	-	chr3.2:55905020-55905039	MS.gene03923:CDS	35.0%
	ATTGGTCATTTCCATCTTTC+TGG	+	chr3.2:55905249-55905268	None:intergenic	35.0%
	GAAGTGTGATATCTAGGTTT+AGG	-	chr3.2:55905117-55905136	MS.gene03923:CDS	35.0%
	TCTTCTTGAGGCAAAACATT+AGG	-	chr3.2:55905186-55905205	MS.gene03923:CDS	35.0%
!	AGAATTTGAAGGAGGTTGAT+CGG	-	chr3.2:55905357-55905376	MS.gene03923:CDS	35.0%
!!	AAGTAAAAAGATGGTGGATC+AGG	-	chr3.2:55905160-55905179	MS.gene03923:CDS	35.0%
	ACATGCTTCTACTCCACATA+GGG	+	chr3.2:55905036-55905055	None:intergenic	40.0%
	ATCAAGAGGAGGTTGGAAAT+GGG	-	chr3.2:55905405-55905424	MS.gene03923:CDS	40.0%
	ATGAAAGATCAAGAGGAGGT+TGG	-	chr3.2:55905398-55905417	MS.gene03923:CDS	40.0%
	GACAATGAAAGATCAAGAGG+AGG	-	chr3.2:55905394-55905413	MS.gene03923:CDS	40.0%
!!	TAAAAAGATGGTGGATCAGG+AGG	-	chr3.2:55905163-55905182	MS.gene03923:CDS	40.0%
	AAGGGAGGAAACCAGAAAGA+TGG	-	chr3.2:55905235-55905254	MS.gene03923:CDS	45.0%
	ACGAGCAGTTGAAGAAGCAA+AGG	-	chr3.2:55905216-55905235	MS.gene03923:CDS	45.0%
	AGGTTTGGCCATCAGATTCA+GGG	-	chr3.2:55905090-55905109	MS.gene03923:CDS	45.0%
	CACATGCTTCTACTCCACAT+AGG	+	chr3.2:55905037-55905056	None:intergenic	45.0%
	CGAGCAGTTGAAGAAGCAAA+GGG	-	chr3.2:55905217-55905236	MS.gene03923:CDS	45.0%
	GATCAAGAGGAGGTTGGAAA+TGG	-	chr3.2:55905404-55905423	MS.gene03923:CDS	45.0%
	TCAAGAGGAGGTTGGAAATG+GGG	-	chr3.2:55905406-55905425	MS.gene03923:CDS	45.0%
	TCTAGGTTTAGGAGTTTGCC+TGG	-	chr3.2:55905128-55905147	MS.gene03923:CDS	45.0%
!	GGGTCAGAAGTGTGATATCT+AGG	-	chr3.2:55905111-55905130	MS.gene03923:CDS	45.0%
	ACAACCCTCGAGTAGAGGTT+TGG	-	chr3.2:55905075-55905094	MS.gene03923:CDS	50.0%
	ATGGCCAAACCTCTACTCGA+GGG	+	chr3.2:55905082-55905101	None:intergenic	50.0%
	CTTCTGACCCCTGAATCTGA+TGG	+	chr3.2:55905101-55905120	None:intergenic	50.0%
	GAGGTTTGGCCATCAGATTC+AGG	-	chr3.2:55905089-55905108	MS.gene03923:CDS	50.0%
	GCAAAACAACCCTCGAGTAG+AGG	-	chr3.2:55905070-55905089	MS.gene03923:CDS	50.0%
	GCAGTTGAAGAAGCAAAGGG+AGG	-	chr3.2:55905220-55905239	MS.gene03923:CDS	50.0%
	GGTTTGGCCATCAGATTCAG+GGG	-	chr3.2:55905091-55905110	MS.gene03923:CDS	50.0%
	TGGATCAGGAGGTCTTCTTG+AGG	-	chr3.2:55905174-55905193	MS.gene03923:CDS	50.0%
	AGAGGAGGTTGGAAATGGGG+TGG	-	chr3.2:55905409-55905428	MS.gene03923:CDS	55.0%
	GAGGAGGTTGGAAATGGGGT+GGG	-	chr3.2:55905410-55905429	MS.gene03923:CDS	55.0%
	GATGGCCAAACCTCTACTCG+AGG	+	chr3.2:55905083-55905102	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	55905008	55905439	55905008	ID=MS.gene03923
chr3.2	mRNA	55905008	55905439	55905008	ID=MS.gene03923.t1;Parent=MS.gene03923
chr3.2	exon	55905008	55905439	55905008	ID=MS.gene03923.t1.exon1;Parent=MS.gene03923.t1
chr3.2	CDS	55905008	55905439	55905008	ID=cds.MS.gene03923.t1;Parent=MS.gene03923.t1

Gene Sequence

>MS.gene03923

ATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTAGAAGCATGTGCAATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGTTTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAACATTAGGAAAGTCTACGAGCAGTTGAAGAAGCAAAGGGAGGAAACCAGAAAGATGGAAATGACCAATATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGAGCAAATCTGATCTTAATGATTTTTCATCTTTTATTGATGAGAATTTGAAGGAGGTTGATCGGAAGATGAAAGAAATGACAATGAAAGATCAAGAGGAGGTTGGAAATGGGGTGGGATTATGA

Protein sequence

>MS.gene03923.t1

MKKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRFRSLPGHEQSKKMVDQEVFLRQNIRKVYEQLKKQREETRKMEMTNIIDHYIQTIEFNGNSMSKSDLNDFSSFIDENLKEVDRKMKEMTMKDQEEVGNGVGL