AlfalfaGEDB | MS.gene039373

M. sativa cultivar XinJiangDaYe / MS.gene039373

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039373.t1	RZB58432.1	45.6	136	67	4	5	133	10	145	3.00E-23	118.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039373.t1	Q8GY42	32.6	135	78	5	5	133	17	144	7.2e-10	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039373.t1	A0A445GB50	45.6	136	67	4	5	133	10	145	2.1e-23	118.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene039373.t1	TF	NAC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039373.t1	MTR_4g094302	29.921	127	81	4	5	131	12	130	4.73e-14	65.5
MS.gene039373.t1	MTR_4g094302	29.921	127	81	4	5	131	12	130	1.21e-13	65.1
MS.gene039373.t1	MTR_4g101680	32.143	140	82	5	5	137	8	141	1.54e-13	66.6
MS.gene039373.t1	MTR_2g080010	30.882	136	82	4	5	134	16	145	2.42e-13	65.9
MS.gene039373.t1	MTR_8g059170	35.036	137	71	7	5	133	15	141	2.67e-13	65.5
MS.gene039373.t1	MTR_5g021710	31.655	139	84	4	5	137	9	142	3.16e-13	65.5
MS.gene039373.t1	MTR_8g094580	33.333	135	75	7	5	133	8	133	7.23e-13	64.3
MS.gene039373.t1	MTR_2g079990	33.333	138	74	7	5	134	17	144	2.42e-12	63.2
MS.gene039373.t1	MTR_2g079990	33.333	138	74	7	5	134	17	144	2.63e-12	63.2
MS.gene039373.t1	MTR_3g096920	33.577	137	72	8	5	133	8	133	4.75e-12	62.0
MS.gene039373.t1	MTR_3g096920	33.577	137	72	8	5	133	8	133	5.96e-12	62.0
MS.gene039373.t1	MTR_1g008740	32.090	134	78	6	5	131	14	141	1.24e-11	60.8
MS.gene039373.t1	MTR_1g008740	32.090	134	78	6	5	131	28	155	1.30e-11	60.8
MS.gene039373.t1	MTR_2g068880	32.143	140	84	6	2	134	20	155	4.02e-11	59.3
MS.gene039373.t1	MTR_3g088110	31.618	136	76	6	5	133	8	133	4.95e-11	58.9
MS.gene039373.t1	MTR_4g134460	33.333	138	75	6	2	131	52	180	6.05e-11	59.3
MS.gene039373.t1	MTR_2g064090	30.000	140	84	6	5	137	7	139	7.60e-11	58.9
MS.gene039373.t1	MTR_6g011860	31.343	134	75	7	6	131	17	141	8.15e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039373.t1	AT4G36160	31.973	147	83	6	5	139	15	156	7.16e-14	67.4
MS.gene039373.t1	AT4G36160	31.973	147	83	6	5	139	11	152	7.75e-14	67.4
MS.gene039373.t1	AT4G36160	31.973	147	83	6	5	139	11	152	7.75e-14	67.4
MS.gene039373.t1	AT3G15500	33.333	135	74	7	5	132	15	140	1.80e-13	66.2
MS.gene039373.t1	AT4G27410	32.000	150	73	7	5	132	15	157	4.64e-13	65.1
MS.gene039373.t1	AT5G62380	33.099	142	78	6	5	137	8	141	5.73e-13	64.7
MS.gene039373.t1	AT5G62380	33.099	142	78	6	5	137	8	141	5.73e-13	64.7
MS.gene039373.t1	AT1G76420	32.143	140	75	6	5	134	23	152	7.21e-13	64.3
MS.gene039373.t1	AT4G17980	29.710	138	81	4	5	133	7	137	1.22e-12	63.5
MS.gene039373.t1	AT4G17980	29.710	138	81	4	5	133	7	137	1.50e-12	63.5
MS.gene039373.t1	AT5G08790	34.559	136	72	7	5	133	8	133	2.12e-12	62.8
MS.gene039373.t1	AT1G52890	32.593	135	75	7	5	132	15	140	2.53e-12	62.8
MS.gene039373.t1	AT5G63790	36.029	136	70	8	5	133	51	176	2.56e-12	62.8
MS.gene039373.t1	AT5G63790	36.029	136	70	8	5	133	61	186	2.72e-12	62.8
MS.gene039373.t1	AT5G04395	30.667	150	84	6	5	137	29	175	3.88e-12	60.8
MS.gene039373.t1	AT1G77450	35.766	137	69	7	5	133	11	136	4.53e-12	61.6
MS.gene039373.t1	AT5G64530	32.283	127	74	4	5	131	4	118	4.97e-12	60.1
MS.gene039373.t1	AT1G26870	29.630	135	81	5	2	129	14	141	8.31e-12	61.6
MS.gene039373.t1	AT1G01720	32.847	137	73	6	5	133	8	133	8.54e-12	61.2
MS.gene039373.t1	AT5G64530	32.283	127	74	4	5	131	4	118	1.16e-11	59.7
MS.gene039373.t1	AT5G50820	32.593	135	75	6	5	134	18	141	2.89e-11	58.9
MS.gene039373.t1	AT3G29035	31.343	134	76	6	5	131	25	149	5.38e-11	59.3
MS.gene039373.t1	AT3G10490	31.343	134	78	6	6	132	29	155	9.44e-11	57.8

Find 30 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATTGTAAGACTGGATTT+TGG	0.240614	6.3:+44216938	MS.gene039373:CDS
CCTCCTCGGCTCCTCAGTTT+TGG	0.293397	6.3:-44216917	None:intergenic
CCATGGATCTGACCGGATTA+AGG	0.314578	6.3:+44216804	MS.gene039373:CDS
CCTTGTGTGAGGGAAGAAAA+AGG	0.356880	6.3:-44216875	None:intergenic
TGTTGGAGCTGATGACGTTT+TGG	0.393392	6.3:+44216990	MS.gene039373:CDS
ACAGTCTCAGCTCCTTAATC+CGG	0.483053	6.3:-44216816	None:intergenic
CCGAGGAGGCATTGTAAGAC+TGG	0.484776	6.3:+44216931	MS.gene039373:CDS
TGAGCAAGATGTAATTCAAA+AGG	0.487325	6.3:-44216850	None:intergenic
GAAGAGGTTCATAATTTCCA+TGG	0.505409	6.3:+44216787	MS.gene039373:CDS
TTGTTCATCGGAAGGGAAGA+AGG	0.508266	6.3:-44216734	None:intergenic
AGAACGCGATCAAAACTAGT+TGG	0.526855	6.3:+44217058	MS.gene039373:CDS
CCTTAATCCGGTCAGATCCA+TGG	0.527947	6.3:-44216804	None:intergenic
GCTGTTAGAGACATTACTGT+TGG	0.528259	6.3:+44216973	MS.gene039373:CDS
CACTCTTGTTAACAATCCTC+AGG	0.528444	6.3:+44217095	MS.gene039373:CDS
GCAATGGATTTGCCTCCACC+TGG	0.541606	6.3:+44216706	None:intergenic
CCAGTCTTACAATGCCTCCT+CGG	0.543084	6.3:-44216931	None:intergenic
ATAATTTCCATGGATCTGAC+CGG	0.543383	6.3:+44216797	MS.gene039373:CDS
AAGAAGGCAACACCAGGTGG+AGG	0.559720	6.3:-44216718	None:intergenic
CAGTAAAACTCCCTTGTGTG+AGG	0.577220	6.3:-44216886	None:intergenic
GAAGGGAAGAAGGCAACACC+AGG	0.588550	6.3:-44216724	None:intergenic
GGGAAGAAGGCAACACCAGG+TGG	0.590123	6.3:-44216721	None:intergenic
TAACATGTTGTTCATCGGAA+GGG	0.594831	6.3:-44216741	None:intergenic
CTAACATGTTGTTCATCGGA+AGG	0.597677	6.3:-44216742	None:intergenic
GTAGCTAACATGTTGTTCAT+CGG	0.601484	6.3:-44216746	None:intergenic
AGTAAAACTCCCTTGTGTGA+GGG	0.603789	6.3:-44216885	None:intergenic
GTAATTCAAAAGGATCATAC+CGG	0.608058	6.3:-44216840	None:intergenic
TTAAGGAGCTGAGACTGTAC+CGG	0.611881	6.3:+44216821	MS.gene039373:CDS
CTGCCAAAACTGAGGAGCCG+AGG	0.619650	6.3:+44216914	MS.gene039373:CDS
CCAAAACTGAGGAGCCGAGG+AGG	0.634705	6.3:+44216917	MS.gene039373:CDS
CTGCTTCACTGCCAAAACTG+AGG	0.647730	6.3:+44216906	MS.gene039373:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTAATATAAATAATT+GGG	+	chr6.3:44217200-44217219	MS.gene039373:intron	0.0%
!!!	TTTTTTTTAATATAAATAAT+TGG	+	chr6.3:44217199-44217218	MS.gene039373:intron	0.0%
!!	TCGATGATTAATTTATGTAT+TGG	+	chr6.3:44217162-44217181	MS.gene039373:intron	20.0%
!!	TTTAATATAAATAATTGGGC+AGG	+	chr6.3:44217204-44217223	MS.gene039373:intron	20.0%
!!!	GATTAATTTATGTATTGGTA+AGG	+	chr6.3:44217167-44217186	MS.gene039373:intron	20.0%
!!	TCTCTCTTCATTTTCTATAT+AGG	+	chr6.3:44217024-44217043	MS.gene039373:CDS	25.0%
	AAAACACAGCAAACAACAAA+AGG	-	chr6.3:44217232-44217251	None:intergenic	30.0%
	GTAATTCAAAAGGATCATAC+CGG	-	chr6.3:44216843-44216862	None:intergenic	30.0%
	TCGATGAAATAACGAACATA+CGG	-	chr6.3:44217146-44217165	None:intergenic	30.0%
	TGAGCAAGATGTAATTCAAA+AGG	-	chr6.3:44216853-44216872	None:intergenic	30.0%
	ATAATTTCCATGGATCTGAC+CGG	+	chr6.3:44216797-44216816	MS.gene039373:CDS	35.0%
	GAAGAGGTTCATAATTTCCA+TGG	+	chr6.3:44216787-44216806	MS.gene039373:CDS	35.0%
	GTAGCTAACATGTTGTTCAT+CGG	-	chr6.3:44216749-44216768	None:intergenic	35.0%
	TAACATGTTGTTCATCGGAA+GGG	-	chr6.3:44216744-44216763	None:intergenic	35.0%
!	AATCCTCAGGTATGTCATTT+GGG	+	chr6.3:44217108-44217127	MS.gene039373:intron	35.0%
!!!	AACCTCTTCGTTGTTTTTGT+TGG	-	chr6.3:44216776-44216795	None:intergenic	35.0%
	AGAACGCGATCAAAACTAGT+TGG	+	chr6.3:44217058-44217077	MS.gene039373:CDS	40.0%
	AGTAAAACTCCCTTGTGTGA+GGG	-	chr6.3:44216888-44216907	None:intergenic	40.0%
	CAATCCTCAGGTATGTCATT+TGG	+	chr6.3:44217107-44217126	MS.gene039373:intron	40.0%
	CACTCTTGTTAACAATCCTC+AGG	+	chr6.3:44217095-44217114	MS.gene039373:CDS	40.0%
	CTAACATGTTGTTCATCGGA+AGG	-	chr6.3:44216745-44216764	None:intergenic	40.0%
	GCTGTTAGAGACATTACTGT+TGG	+	chr6.3:44216973-44216992	MS.gene039373:CDS	40.0%
	TGCCAACAAAAACAACGAAG+AGG	+	chr6.3:44216771-44216790	MS.gene039373:CDS	40.0%
	TTTCCCAAATGACATACCTG+AGG	-	chr6.3:44217114-44217133	None:intergenic	40.0%
!	ACTGGATTTTGGATGCAGAA+GGG	+	chr6.3:44216949-44216968	MS.gene039373:CDS	40.0%
!	CTTTTTCTTCCCTCACACAA+GGG	+	chr6.3:44216876-44216895	MS.gene039373:CDS	40.0%
!	GGCATTGTAAGACTGGATTT+TGG	+	chr6.3:44216938-44216957	MS.gene039373:CDS	40.0%
!!	GTGTTTTTGACTGCCCTAAA+GGG	+	chr6.3:44217245-44217264	MS.gene039373:intron	40.0%
!!	TGTGTTTTTGACTGCCCTAA+AGG	+	chr6.3:44217244-44217263	MS.gene039373:intron	40.0%
!!!	CTTCGTTGTTTTTGTTGGCA+AGG	-	chr6.3:44216771-44216790	None:intergenic	40.0%
!!!	TAGTTTTGATCGCGTTCTGA+GGG	-	chr6.3:44217056-44217075	None:intergenic	40.0%
	ACAGTCTCAGCTCCTTAATC+CGG	-	chr6.3:44216819-44216838	None:intergenic	45.0%
	CAGTAAAACTCCCTTGTGTG+AGG	-	chr6.3:44216889-44216908	None:intergenic	45.0%
	CCTTGTGTGAGGGAAGAAAA+AGG	-	chr6.3:44216878-44216897	None:intergenic	45.0%
	TTAAGGAGCTGAGACTGTAC+CGG	+	chr6.3:44216821-44216840	MS.gene039373:CDS	45.0%
	TTGTTCATCGGAAGGGAAGA+AGG	-	chr6.3:44216737-44216756	None:intergenic	45.0%
!	CCTTTTTCTTCCCTCACACA+AGG	+	chr6.3:44216875-44216894	MS.gene039373:CDS	45.0%
!	GACTGGATTTTGGATGCAGA+AGG	+	chr6.3:44216948-44216967	MS.gene039373:CDS	45.0%
!!	AGTTTTGATCGCGTTCTGAG+GGG	-	chr6.3:44217055-44217074	None:intergenic	45.0%
!!	TGTTGGAGCTGATGACGTTT+TGG	+	chr6.3:44216990-44217009	MS.gene039373:CDS	45.0%
!!	TTTTGACTGCCCTAAAGGGA+TGG	+	chr6.3:44217249-44217268	MS.gene039373:intron	45.0%
!!!	CTAGTTTTGATCGCGTTCTG+AGG	-	chr6.3:44217057-44217076	None:intergenic	45.0%
	CCAGTCTTACAATGCCTCCT+CGG	-	chr6.3:44216934-44216953	None:intergenic	50.0%
	CCATGGATCTGACCGGATTA+AGG	+	chr6.3:44216804-44216823	MS.gene039373:CDS	50.0%
	CCTTAATCCGGTCAGATCCA+TGG	-	chr6.3:44216807-44216826	None:intergenic	50.0%
	CTGCTTCACTGCCAAAACTG+AGG	+	chr6.3:44216906-44216925	MS.gene039373:CDS	50.0%
!!	GGAGCTGATGACGTTTTGGT+GGG	+	chr6.3:44216994-44217013	MS.gene039373:CDS	50.0%
!!	TGGAGCTGATGACGTTTTGG+TGG	+	chr6.3:44216993-44217012	MS.gene039373:CDS	50.0%
	AAGAAGGCAACACCAGGTGG+AGG	-	chr6.3:44216721-44216740	None:intergenic	55.0%
	GAAGGGAAGAAGGCAACACC+AGG	-	chr6.3:44216727-44216746	None:intergenic	55.0%
!!	CCGAGGAGGCATTGTAAGAC+TGG	+	chr6.3:44216931-44216950	MS.gene039373:CDS	55.0%
	CCAAAACTGAGGAGCCGAGG+AGG	+	chr6.3:44216917-44216936	MS.gene039373:CDS	60.0%
	CTGCCAAAACTGAGGAGCCG+AGG	+	chr6.3:44216914-44216933	MS.gene039373:CDS	60.0%
	GGGAAGAAGGCAACACCAGG+TGG	-	chr6.3:44216724-44216743	None:intergenic	60.0%
!	CCTCCTCGGCTCCTCAGTTT+TGG	-	chr6.3:44216920-44216939	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	44216709	44217277	44216709	ID=MS.gene039373
chr6.3	mRNA	44216709	44217277	44216709	ID=MS.gene039373.t1;Parent=MS.gene039373
chr6.3	exon	44216709	44217116	44216709	ID=MS.gene039373.t1.exon1;Parent=MS.gene039373.t1
chr6.3	CDS	44216709	44217116	44216709	ID=cds.MS.gene039373.t1;Parent=MS.gene039373.t1
chr6.3	exon	44217266	44217277	44217266	ID=MS.gene039373.t1.exon2;Parent=MS.gene039373.t1
chr6.3	CDS	44217266	44217277	44217266	ID=cds.MS.gene039373.t1;Parent=MS.gene039373.t1

Gene Sequence

>MS.gene039373

ATGGATTTGCCTCCACCTGGTGTTGCCTTCTTCCCTTCCGATGAACAACATGTTAGCTACCTTGCCAACAAAAACAACGAAGAGGTTCATAATTTCCATGGATCTGACCGGATTAAGGAGCTGAGACTGTACCGGTATGATCCTTTTGAATTACATCTTGCTCAGTCCTTTTTCTTCCCTCACACAAGGGAGTTTTACTGCTTCACTGCCAAAACTGAGGAGCCGAGGAGGCATTGTAAGACTGGATTTTGGATGCAGAAGGGAGCTGTTAGAGACATTACTGTTGGAGCTGATGACGTTTTGGTGGGAAAGAAGTCTCTCTTCATTTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGTTAACAATCCTCAGGTATGTCATTTGGGAAAACTAACTTTCCGTATGTTCGTTATTTCATCGATGATTAATTTATGTATTGGTAAGGACTAACTATTTTTTTTTAATATAAATAATTGGGCAGGCTCCTTTTGTTGTTTGCTGTGTTTTTGACTGCCCTAAAGGGATGGATCTAG

Protein sequence

>MS.gene039373.t1

MDLPPPGVAFFPSDEQHVSYLANKNNEEVHNFHGSDRIKELRLYRYDPFELHLAQSFFFPHTREFYCFTAKTEEPRRHCKTGFWMQKGAVRDITVGADDVLVGKKSLFIFYIGNSPQNAIKTSWTMYEYTLVNNPQGWI