AlfalfaGEDB | MS.gene039374

M. sativa cultivar XinJiangDaYe / MS.gene039374

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039374.t1	RZB58432.1	45.6	136	67	4	5	133	10	145	4.60E-24	120.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039374.t1	Q8GY42	32.6	135	78	5	5	133	17	144	1.4e-10	67.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039374.t1	A0A445GB50	45.6	136	67	4	5	133	10	145	3.3e-24	120.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene039374.t1	TF	NAC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039374.t1	MTR_4g094302	29.921	127	81	4	5	131	12	130	1.10e-14	67.0
MS.gene039374.t1	MTR_4g094302	29.921	127	81	4	5	131	12	130	2.99e-14	66.6
MS.gene039374.t1	MTR_4g101680	31.655	139	84	4	5	137	8	141	5.17e-14	67.8
MS.gene039374.t1	MTR_2g080010	30.882	136	82	4	5	134	16	145	8.70e-14	67.0
MS.gene039374.t1	MTR_8g059170	35.036	137	71	7	5	133	15	141	9.33e-14	67.0
MS.gene039374.t1	MTR_5g021710	31.655	139	84	4	5	137	9	142	1.15e-13	66.6
MS.gene039374.t1	MTR_4g089135	32.593	135	77	5	5	132	16	143	1.42e-13	66.6
MS.gene039374.t1	MTR_5g012080	31.944	144	78	7	5	137	8	142	1.51e-13	66.2
MS.gene039374.t1	MTR_7g105170	28.058	139	91	4	1	132	1	137	3.97e-13	64.7
MS.gene039374.t1	MTR_1g096430	29.771	131	81	4	5	129	8	133	7.81e-13	64.3
MS.gene039374.t1	MTR_2g079990	33.333	138	74	7	5	134	17	144	9.09e-13	64.3
MS.gene039374.t1	MTR_2g079990	33.333	138	74	7	5	134	17	144	9.64e-13	64.3
MS.gene039374.t1	MTR_3g088110	31.618	136	76	6	5	133	8	133	1.58e-11	60.5
MS.gene039374.t1	MTR_2g086880	28.986	138	73	7	6	131	20	144	2.61e-11	60.1
MS.gene039374.t1	MTR_2g086880	28.986	138	73	7	6	131	20	144	3.17e-11	60.1
MS.gene039374.t1	MTR_6g011860	30.075	133	78	6	6	131	17	141	3.44e-11	59.7
MS.gene039374.t1	MTR_3g064580	30.435	138	74	6	5	131	7	133	5.03e-11	59.3
MS.gene039374.t1	MTR_2g068920	31.429	140	85	6	2	134	19	154	9.26e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039374.t1	AT4G36160	31.973	147	83	6	5	139	15	156	2.56e-14	68.9
MS.gene039374.t1	AT4G36160	31.973	147	83	6	5	139	11	152	2.74e-14	68.6
MS.gene039374.t1	AT4G36160	31.973	147	83	6	5	139	11	152	2.74e-14	68.6
MS.gene039374.t1	AT1G12260	32.639	144	76	7	5	137	8	141	7.23e-14	67.4
MS.gene039374.t1	AT1G12260	33.099	142	78	6	5	137	8	141	9.39e-14	67.0
MS.gene039374.t1	AT5G62380	33.099	142	78	6	5	137	8	141	1.58e-13	66.2
MS.gene039374.t1	AT5G62380	33.099	142	78	6	5	137	8	141	1.58e-13	66.2
MS.gene039374.t1	AT2G18060	31.293	147	84	6	5	139	10	151	1.80e-13	66.2
MS.gene039374.t1	AT2G18060	31.293	147	84	6	5	139	10	151	1.80e-13	66.2
MS.gene039374.t1	AT1G71930	27.211	147	90	5	5	139	10	151	1.82e-13	66.2
MS.gene039374.t1	AT1G71930	27.211	147	90	5	5	139	10	151	1.82e-13	66.2
MS.gene039374.t1	AT4G27410	32.000	150	73	7	5	132	15	157	1.89e-13	65.9
MS.gene039374.t1	AT4G17980	29.710	138	81	4	5	133	7	137	2.49e-13	65.1
MS.gene039374.t1	AT4G17980	29.710	138	81	4	5	133	7	137	3.09e-13	65.1
MS.gene039374.t1	AT5G08790	34.559	136	72	7	5	133	8	133	8.68e-13	63.9
MS.gene039374.t1	AT5G63790	36.029	136	70	8	5	133	51	176	9.48e-13	63.9
MS.gene039374.t1	AT5G64530	32.283	127	74	4	5	131	4	118	9.49e-13	62.0
MS.gene039374.t1	AT5G63790	36.029	136	70	8	5	133	61	186	1.02e-12	63.9
MS.gene039374.t1	AT3G15500	31.852	135	76	6	5	132	15	140	1.05e-12	63.9
MS.gene039374.t1	AT1G62700	31.293	147	74	6	5	137	8	141	1.13e-12	63.9
MS.gene039374.t1	AT1G62700	31.293	147	74	6	5	137	8	141	1.13e-12	63.9
MS.gene039374.t1	AT5G04395	30.667	150	84	6	5	137	29	175	1.41e-12	62.0
MS.gene039374.t1	AT5G64530	32.283	127	74	4	5	131	4	118	2.30e-12	61.6
MS.gene039374.t1	AT1G01720	32.847	137	73	6	5	133	8	133	3.48e-12	62.4
MS.gene039374.t1	AT1G32510	29.371	143	82	5	5	134	7	143	4.14e-12	62.0
MS.gene039374.t1	AT1G52890	32.353	136	74	7	5	132	15	140	4.19e-12	62.4
MS.gene039374.t1	AT3G44290	25.758	132	89	4	6	133	16	142	5.67e-12	61.2
MS.gene039374.t1	AT3G44290	25.954	131	88	4	6	132	16	141	8.04e-12	61.6
MS.gene039374.t1	AT5G50820	32.593	135	75	6	5	134	18	141	9.83e-12	60.1
MS.gene039374.t1	AT2G27300	25.926	135	91	4	6	136	16	145	1.96e-11	60.5
MS.gene039374.t1	AT5G46590	29.412	136	85	5	5	134	7	137	2.35e-11	60.1
MS.gene039374.t1	AT5G46590	29.412	136	85	5	5	134	7	137	2.44e-11	60.1
MS.gene039374.t1	AT3G29035	31.343	134	76	6	5	131	25	149	3.67e-11	59.7
MS.gene039374.t1	AT5G17260	28.889	135	80	6	5	131	7	133	3.70e-11	59.7
MS.gene039374.t1	AT1G26870	28.358	134	84	4	2	129	14	141	4.30e-11	59.7

Find 30 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATTGTAAGACTGGATTT+TGG	0.240614	6.3:+44259917	MS.gene039374:CDS
CCATGGTTCTGACCGGATTA+AGG	0.270785	6.3:+44259783	MS.gene039374:CDS
CCTCCTCGGCTCCTCAGTTT+TGG	0.293397	6.3:-44259896	None:intergenic
CCTTGTGTGAGGGAAGAAAA+AGG	0.356880	6.3:-44259854	None:intergenic
TGTTGGAGCTGATGACGTTT+TGG	0.393392	6.3:+44259969	MS.gene039374:CDS
ACAGTCTCAGCTCCTTAATC+CGG	0.483053	6.3:-44259795	None:intergenic
CCGAGGAGGCATTGTAAGAC+TGG	0.484776	6.3:+44259910	MS.gene039374:CDS
TGAGCAAGATGTAATTCAAA+AGG	0.487325	6.3:-44259829	None:intergenic
TTGTTCATCGGAAGGGAAGA+AGG	0.494722	6.3:-44259713	None:intergenic
AGAACGCGATCAAAACTAGT+TGG	0.526855	6.3:+44260037	MS.gene039374:CDS
GCTGTTAGAGACATTACTGT+TGG	0.528259	6.3:+44259952	MS.gene039374:CDS
CACTCTTGTTAACAATCCTC+AGG	0.528444	6.3:+44260074	MS.gene039374:CDS
AGAATTTCCATGGTTCTGAC+CGG	0.533129	6.3:+44259776	MS.gene039374:CDS
GAAGAGGTTCAGAATTTCCA+TGG	0.534682	6.3:+44259766	MS.gene039374:CDS
GCAATGGATTTGCCTCCACC+TGG	0.541606	6.3:+44259685	None:intergenic
CCAGTCTTACAATGCCTCCT+CGG	0.543084	6.3:-44259910	None:intergenic
AAGAAGGCAACACCAGGTGG+AGG	0.559720	6.3:-44259697	None:intergenic
CAGTAAAACTCCCTTGTGTG+AGG	0.577220	6.3:-44259865	None:intergenic
CCTTAATCCGGTCAGAACCA+TGG	0.580530	6.3:-44259783	None:intergenic
GAAGGGAAGAAGGCAACACC+AGG	0.588550	6.3:-44259703	None:intergenic
GGGAAGAAGGCAACACCAGG+TGG	0.590123	6.3:-44259700	None:intergenic
GTAGCTAACAAGTTGTTCAT+CGG	0.592426	6.3:-44259725	None:intergenic
TAACAAGTTGTTCATCGGAA+GGG	0.594831	6.3:-44259720	None:intergenic
CTAACAAGTTGTTCATCGGA+AGG	0.597677	6.3:-44259721	None:intergenic
AGTAAAACTCCCTTGTGTGA+GGG	0.603789	6.3:-44259864	None:intergenic
GTAATTCAAAAGGATCATAC+CGG	0.608058	6.3:-44259819	None:intergenic
TTAAGGAGCTGAGACTGTAC+CGG	0.611881	6.3:+44259800	MS.gene039374:CDS
CTGCCAAAACTGAGGAGCCG+AGG	0.619650	6.3:+44259893	MS.gene039374:CDS
CCAAAACTGAGGAGCCGAGG+AGG	0.634705	6.3:+44259896	MS.gene039374:CDS
CTGCTTCACTGCCAAAACTG+AGG	0.647730	6.3:+44259885	MS.gene039374:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTAATATAAATAATT+GGG	+	chr6.3:44260179-44260198	MS.gene039374:intron	0.0%
!!!	TTTTTTTTAATATAAATAAT+TGG	+	chr6.3:44260178-44260197	MS.gene039374:intron	0.0%
!!	TCGATGATTAATTTATGTAT+TGG	+	chr6.3:44260141-44260160	MS.gene039374:intron	20.0%
!!	TTTAATATAAATAATTGGGC+AGG	+	chr6.3:44260183-44260202	MS.gene039374:intron	20.0%
!!!	GATTAATTTATGTATTGGTA+AGG	+	chr6.3:44260146-44260165	MS.gene039374:intron	20.0%
!!	TCTCTCTTCATTTTCTATAT+AGG	+	chr6.3:44260003-44260022	MS.gene039374:CDS	25.0%
	AAAACACAGCAAACAACAAA+AGG	-	chr6.3:44260211-44260230	None:intergenic	30.0%
	GTAATTCAAAAGGATCATAC+CGG	-	chr6.3:44259822-44259841	None:intergenic	30.0%
	TCGATGAAATAACGAACATA+CGG	-	chr6.3:44260125-44260144	None:intergenic	30.0%
	TGAGCAAGATGTAATTCAAA+AGG	-	chr6.3:44259832-44259851	None:intergenic	30.0%
	GTAGCTAACAAGTTGTTCAT+CGG	-	chr6.3:44259728-44259747	None:intergenic	35.0%
	TAACAAGTTGTTCATCGGAA+GGG	-	chr6.3:44259723-44259742	None:intergenic	35.0%
!	AATCCTCAGGTATGTCATTT+GGG	+	chr6.3:44260087-44260106	MS.gene039374:intron	35.0%
!!!	AACCTCTTCGTTGTTTTTGT+TGG	-	chr6.3:44259755-44259774	None:intergenic	35.0%
	AGAACGCGATCAAAACTAGT+TGG	+	chr6.3:44260037-44260056	MS.gene039374:CDS	40.0%
	AGTAAAACTCCCTTGTGTGA+GGG	-	chr6.3:44259867-44259886	None:intergenic	40.0%
	CAATCCTCAGGTATGTCATT+TGG	+	chr6.3:44260086-44260105	MS.gene039374:intron	40.0%
	CACTCTTGTTAACAATCCTC+AGG	+	chr6.3:44260074-44260093	MS.gene039374:CDS	40.0%
	CTAACAAGTTGTTCATCGGA+AGG	-	chr6.3:44259724-44259743	None:intergenic	40.0%
	GAAGAGGTTCAGAATTTCCA+TGG	+	chr6.3:44259766-44259785	MS.gene039374:CDS	40.0%
	GCTGTTAGAGACATTACTGT+TGG	+	chr6.3:44259952-44259971	MS.gene039374:CDS	40.0%
	TGCCAACAAAAACAACGAAG+AGG	+	chr6.3:44259750-44259769	MS.gene039374:CDS	40.0%
	TTTCCCAAATGACATACCTG+AGG	-	chr6.3:44260093-44260112	None:intergenic	40.0%
!	ACTGGATTTTGGATGCAGAA+GGG	+	chr6.3:44259928-44259947	MS.gene039374:CDS	40.0%
!	CTTTTTCTTCCCTCACACAA+GGG	+	chr6.3:44259855-44259874	MS.gene039374:CDS	40.0%
!	GGCATTGTAAGACTGGATTT+TGG	+	chr6.3:44259917-44259936	MS.gene039374:CDS	40.0%
!!	AGAATTTCCATGGTTCTGAC+CGG	+	chr6.3:44259776-44259795	MS.gene039374:CDS	40.0%
!!	GTGTTTTTGACTGCCCTAAA+GGG	+	chr6.3:44260224-44260243	MS.gene039374:intron	40.0%
!!	TGTGTTTTTGACTGCCCTAA+AGG	+	chr6.3:44260223-44260242	MS.gene039374:intron	40.0%
!!!	CTTCGTTGTTTTTGTTGGCA+AGG	-	chr6.3:44259750-44259769	None:intergenic	40.0%
!!!	TAGTTTTGATCGCGTTCTGA+GGG	-	chr6.3:44260035-44260054	None:intergenic	40.0%
	ACAGTCTCAGCTCCTTAATC+CGG	-	chr6.3:44259798-44259817	None:intergenic	45.0%
	CAGTAAAACTCCCTTGTGTG+AGG	-	chr6.3:44259868-44259887	None:intergenic	45.0%
	CCTTGTGTGAGGGAAGAAAA+AGG	-	chr6.3:44259857-44259876	None:intergenic	45.0%
	TTAAGGAGCTGAGACTGTAC+CGG	+	chr6.3:44259800-44259819	MS.gene039374:CDS	45.0%
	TTGTTCATCGGAAGGGAAGA+AGG	-	chr6.3:44259716-44259735	None:intergenic	45.0%
!	CCTTTTTCTTCCCTCACACA+AGG	+	chr6.3:44259854-44259873	MS.gene039374:CDS	45.0%
!	GACTGGATTTTGGATGCAGA+AGG	+	chr6.3:44259927-44259946	MS.gene039374:CDS	45.0%
!!	AGTTTTGATCGCGTTCTGAG+GGG	-	chr6.3:44260034-44260053	None:intergenic	45.0%
!!	TGTTGGAGCTGATGACGTTT+TGG	+	chr6.3:44259969-44259988	MS.gene039374:CDS	45.0%
!!	TTTTGACTGCCCTAAAGGGA+TGG	+	chr6.3:44260228-44260247	MS.gene039374:intron	45.0%
!!!	CTAGTTTTGATCGCGTTCTG+AGG	-	chr6.3:44260036-44260055	None:intergenic	45.0%
	CCAGTCTTACAATGCCTCCT+CGG	-	chr6.3:44259913-44259932	None:intergenic	50.0%
	CCTTAATCCGGTCAGAACCA+TGG	-	chr6.3:44259786-44259805	None:intergenic	50.0%
	CTGCTTCACTGCCAAAACTG+AGG	+	chr6.3:44259885-44259904	MS.gene039374:CDS	50.0%
!!	CCATGGTTCTGACCGGATTA+AGG	+	chr6.3:44259783-44259802	MS.gene039374:CDS	50.0%
!!	GGAGCTGATGACGTTTTGGT+GGG	+	chr6.3:44259973-44259992	MS.gene039374:CDS	50.0%
!!	TGGAGCTGATGACGTTTTGG+TGG	+	chr6.3:44259972-44259991	MS.gene039374:CDS	50.0%
	AAGAAGGCAACACCAGGTGG+AGG	-	chr6.3:44259700-44259719	None:intergenic	55.0%
	GAAGGGAAGAAGGCAACACC+AGG	-	chr6.3:44259706-44259725	None:intergenic	55.0%
!!	CCGAGGAGGCATTGTAAGAC+TGG	+	chr6.3:44259910-44259929	MS.gene039374:CDS	55.0%
	CCAAAACTGAGGAGCCGAGG+AGG	+	chr6.3:44259896-44259915	MS.gene039374:CDS	60.0%
	CTGCCAAAACTGAGGAGCCG+AGG	+	chr6.3:44259893-44259912	MS.gene039374:CDS	60.0%
	GGGAAGAAGGCAACACCAGG+TGG	-	chr6.3:44259703-44259722	None:intergenic	60.0%
!	CCTCCTCGGCTCCTCAGTTT+TGG	-	chr6.3:44259899-44259918	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	44259688	44260256	44259688	ID=MS.gene039374
chr6.3	mRNA	44259688	44260256	44259688	ID=MS.gene039374.t1;Parent=MS.gene039374
chr6.3	exon	44259688	44260095	44259688	ID=MS.gene039374.t1.exon1;Parent=MS.gene039374.t1
chr6.3	CDS	44259688	44260095	44259688	ID=cds.MS.gene039374.t1;Parent=MS.gene039374.t1
chr6.3	exon	44260245	44260256	44260245	ID=MS.gene039374.t1.exon2;Parent=MS.gene039374.t1
chr6.3	CDS	44260245	44260256	44260245	ID=cds.MS.gene039374.t1;Parent=MS.gene039374.t1

Gene Sequence

>MS.gene039374

ATGGATTTGCCTCCACCTGGTGTTGCCTTCTTCCCTTCCGATGAACAACTTGTTAGCTACCTTGCCAACAAAAACAACGAAGAGGTTCAGAATTTCCATGGTTCTGACCGGATTAAGGAGCTGAGACTGTACCGGTATGATCCTTTTGAATTACATCTTGCTCAGTCCTTTTTCTTCCCTCACACAAGGGAGTTTTACTGCTTCACTGCCAAAACTGAGGAGCCGAGGAGGCATTGTAAGACTGGATTTTGGATGCAGAAGGGAGCTGTTAGAGACATTACTGTTGGAGCTGATGACGTTTTGGTGGGAAAGAAGTCTCTCTTCATTTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGTTGGACTATGTACAAGTACACTCTTGTTAACAATCCTCAGGTATGTCATTTGGGAAAACTAACTTTCCGTATGTTCGTTATTTCATCGATGATTAATTTATGTATTGGTAAGGACTAACTATTTTTTTTTAATATAAATAATTGGGCAGGCTCCTTTTGTTGTTTGCTGTGTTTTTGACTGCCCTAAAGGGATGGATCTAG

Protein sequence

>MS.gene039374.t1

MDLPPPGVAFFPSDEQLVSYLANKNNEEVQNFHGSDRIKELRLYRYDPFELHLAQSFFFPHTREFYCFTAKTEEPRRHCKTGFWMQKGAVRDITVGADDVLVGKKSLFIFYIGNSPQNAIKTSWTMYKYTLVNNPQGWI