AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039465

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039465.t1 MTR_4g070320 95.695 302 12 1 1 301 43 344 0.0 597
MS.gene039465.t1 MTR_3g116770 59.434 318 82 11 16 301 95 397 1.61e-114 336
MS.gene039465.t1 MTR_3g116770 59.248 319 82 12 16 301 95 398 1.06e-112 332
MS.gene039465.t1 MTR_7g053410 51.691 207 84 6 11 203 220 424 1.79e-53 182
MS.gene039465.t1 MTR_7g053410 51.691 207 84 6 11 203 220 424 3.57e-53 182
MS.gene039465.t1 MTR_5g048860 46.332 259 102 13 61 301 319 558 6.76e-50 174
MS.gene039465.t1 MTR_8g065740 54.088 159 68 3 30 187 238 392 7.36e-47 164
MS.gene039465.t1 MTR_8g012290 60.000 145 52 3 48 190 242 382 1.31e-46 164
MS.gene039465.t1 MTR_8g012290 60.000 145 52 3 48 190 242 382 1.43e-46 164
MS.gene039465.t1 MTR_1g017350 45.972 211 72 6 80 276 226 408 1.02e-45 160
MS.gene039465.t1 MTR_1g017350 45.972 211 72 6 80 276 226 408 1.19e-45 159
MS.gene039465.t1 MTR_1g017350 65.833 120 27 2 80 185 226 345 8.21e-45 157
MS.gene039465.t1 MTR_1g017350 65.833 120 27 2 80 185 226 345 9.95e-45 157
MS.gene039465.t1 MTR_8g062240 75.258 97 24 0 97 193 148 244 8.13e-44 151
MS.gene039465.t1 MTR_5g037250 59.701 134 42 2 56 189 79 200 2.15e-43 149
MS.gene039465.t1 MTR_7g092510 74.194 93 24 0 93 185 166 258 1.52e-42 149
MS.gene039465.t1 MTR_1g059270 64.220 109 35 1 79 187 143 247 6.43e-42 145
MS.gene039465.t1 MTR_1g059270 62.712 118 35 3 79 192 143 255 7.66e-42 147
MS.gene039465.t1 MTR_8g099880 82.143 84 14 1 97 179 142 225 2.78e-40 143
MS.gene039465.t1 MTR_3g498825 75.000 92 20 1 97 185 144 235 4.84e-40 142
MS.gene039465.t1 MTR_6g084120 55.556 99 34 2 80 177 134 223 8.78e-26 103
MS.gene039465.t1 MTR_4g131160 71.212 66 18 1 100 165 199 263 5.80e-22 95.1
MS.gene039465.t1 MTR_4g131160 71.212 66 18 1 100 165 196 260 5.83e-22 95.1
MS.gene039465.t1 MTR_1g080890 68.182 66 20 1 100 165 193 257 1.04e-20 91.3
MS.gene039465.t1 MTR_4g065870 56.923 65 28 0 100 164 355 419 3.14e-15 75.9
MS.gene039465.t1 MTR_1g069155 52.174 69 32 1 102 170 319 386 5.24e-15 75.5
MS.gene039465.t1 MTR_2g098310 53.846 65 30 0 100 164 332 396 6.51e-15 75.1
MS.gene039465.t1 MTR_4g033295 53.846 65 30 0 100 164 118 182 9.76e-15 71.6
MS.gene039465.t1 MTR_1g084980 45.205 73 39 1 102 174 464 535 1.33e-14 74.3
MS.gene039465.t1 MTR_2g098310 54.688 64 29 0 100 163 332 395 1.70e-14 73.6
MS.gene039465.t1 MTR_4g033295 52.239 67 31 1 100 165 118 184 1.87e-14 70.9
MS.gene039465.t1 MTR_4g065870 56.452 62 27 0 100 161 355 416 2.08e-14 73.6
MS.gene039465.t1 MTR_1g052470 48.718 78 38 1 93 168 289 366 2.47e-14 73.2
MS.gene039465.t1 MTR_1g018090 45.455 88 43 2 77 164 255 337 5.93e-14 72.0
MS.gene039465.t1 MTR_1g070870 63.636 66 23 1 100 165 120 184 6.98e-14 70.9
MS.gene039465.t1 MTR_1g070870 63.636 66 23 1 100 165 123 187 7.70e-14 70.9
MS.gene039465.t1 MTR_7g092540 45.783 83 43 1 93 173 234 316 9.22e-14 70.9
MS.gene039465.t1 MTR_7g099540 50.000 68 33 1 103 170 255 321 1.95e-13 70.9
MS.gene039465.t1 MTR_7g092540 47.945 73 36 1 93 163 234 306 6.31e-13 68.6
MS.gene039465.t1 MTR_7g111320 34.118 170 100 6 66 228 396 560 9.39e-13 68.9
MS.gene039465.t1 MTR_1g019240 40.909 88 50 2 80 166 64 150 1.10e-12 67.4
MS.gene039465.t1 MTR_3g028520 49.231 65 33 0 100 164 322 386 2.11e-12 67.4
MS.gene039465.t1 MTR_3g027650 49.231 65 33 0 100 164 322 386 2.11e-12 67.4
MS.gene039465.t1 MTR_8g036235 47.692 65 34 0 100 164 316 380 5.10e-12 66.2
MS.gene039465.t1 MTR_7g099540 49.206 63 31 1 103 165 255 316 9.32e-12 65.5
MS.gene039465.t1 MTR_3g027650 50.000 62 31 0 100 161 322 383 1.08e-11 65.5
MS.gene039465.t1 MTR_3g449770 36.893 103 60 3 102 201 311 411 1.39e-11 65.1
MS.gene039465.t1 MTR_5g005110 34.146 123 63 3 42 164 183 287 2.42e-11 63.9
MS.gene039465.t1 MTR_3g103030 51.562 64 30 1 101 164 230 292 3.69e-11 63.2
MS.gene039465.t1 MTR_7g110810 43.939 66 36 1 111 176 213 277 3.96e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039465.t1 AT4G34530 53.023 215 68 6 29 212 91 303 2.89e-58 190
MS.gene039465.t1 AT2G18300 59.627 161 55 5 31 190 124 275 7.72e-53 176
MS.gene039465.t1 AT1G68920 39.869 306 136 8 17 300 205 484 4.48e-52 178
MS.gene039465.t1 AT1G68920 41.297 293 124 7 21 300 228 485 5.88e-52 177
MS.gene039465.t1 AT1G68920 41.297 293 124 7 21 300 228 485 5.88e-52 177
MS.gene039465.t1 AT1G68920 41.297 293 124 7 21 300 228 485 5.88e-52 177
MS.gene039465.t1 AT2G18300 60.897 156 51 5 31 185 124 270 8.00e-52 173
MS.gene039465.t1 AT3G07340 42.857 280 124 7 20 286 195 451 1.15e-51 176
MS.gene039465.t1 AT2G18300 60.897 156 51 5 31 185 124 270 1.74e-51 173
MS.gene039465.t1 AT3G07340 53.093 194 70 4 20 213 195 367 1.90e-51 175
MS.gene039465.t1 AT3G23690 56.742 178 67 4 48 223 140 309 1.13e-49 169
MS.gene039465.t1 AT1G26260 53.191 188 64 5 43 219 126 300 2.97e-49 167
MS.gene039465.t1 AT1G26260 53.191 188 64 5 43 219 155 329 9.92e-49 166
MS.gene039465.t1 AT1G26260 57.325 157 56 1 43 199 177 322 1.30e-48 166
MS.gene039465.t1 AT1G26260 57.325 157 56 1 43 199 177 322 1.30e-48 166
MS.gene039465.t1 AT1G26260 57.325 157 56 1 43 199 177 322 1.30e-48 166
MS.gene039465.t1 AT1G26260 57.325 157 56 1 43 199 177 322 1.30e-48 166
MS.gene039465.t1 AT5G48560 50.521 192 89 3 2 187 198 389 1.49e-48 168
MS.gene039465.t1 AT5G48560 50.521 192 89 3 2 187 198 389 3.00e-48 168
MS.gene039465.t1 AT4G36540 53.254 169 58 4 31 199 93 240 5.35e-48 162
MS.gene039465.t1 AT4G36540 56.129 155 47 4 31 185 93 226 3.39e-47 160
MS.gene039465.t1 AT1G10120 46.544 217 78 5 65 281 174 352 9.35e-47 161
MS.gene039465.t1 AT1G10120 46.544 217 78 5 65 281 174 352 9.35e-47 161
MS.gene039465.t1 AT1G59640 63.636 132 41 2 66 191 97 227 1.61e-45 155
MS.gene039465.t1 AT2G42300 71.134 97 28 0 90 186 175 271 1.50e-44 153
MS.gene039465.t1 AT3G57800 63.415 123 40 2 91 213 195 312 6.97e-44 154
MS.gene039465.t1 AT1G59640 65.354 127 37 2 66 186 97 222 7.32e-44 153
MS.gene039465.t1 AT5G50915 54.777 157 53 6 51 201 85 229 1.08e-43 150
MS.gene039465.t1 AT5G50915 54.777 157 53 6 51 201 85 229 1.08e-43 150
MS.gene039465.t1 AT5G50915 54.777 157 53 6 51 201 85 229 4.13e-43 149
MS.gene039465.t1 AT5G50915 54.777 157 53 6 51 201 85 229 4.13e-43 149
MS.gene039465.t1 AT2G42300 61.538 117 41 1 90 206 175 287 6.75e-43 150
MS.gene039465.t1 AT5G62610 77.647 85 15 1 91 171 140 224 3.29e-38 136
MS.gene039465.t1 AT5G62610 77.647 85 15 1 91 171 140 224 3.29e-38 136
MS.gene039465.t1 AT1G18400 76.250 80 19 0 98 177 143 222 3.32e-36 130
MS.gene039465.t1 AT1G18400 76.250 80 19 0 98 177 143 222 6.78e-36 130
MS.gene039465.t1 AT1G73830 77.215 79 18 0 99 177 146 224 1.21e-35 129
MS.gene039465.t1 AT1G73830 77.215 79 18 0 99 177 146 224 1.26e-35 129
MS.gene039465.t1 AT3G57800 45.882 170 40 3 91 213 195 359 5.23e-35 131
MS.gene039465.t1 AT1G25330 64.286 98 34 1 91 187 94 191 1.01e-34 125
MS.gene039465.t1 AT5G58010 60.000 90 28 3 90 175 92 177 2.53e-23 97.4
MS.gene039465.t1 AT2G24260 56.471 85 36 1 81 165 16 99 1.61e-22 94.4
MS.gene039465.t1 AT4G30980 71.212 66 18 1 100 165 130 194 3.30e-22 94.7
MS.gene039465.t1 AT4G30980 71.212 66 18 1 100 165 178 242 4.87e-22 95.1
MS.gene039465.t1 AT2G24260 56.471 85 36 1 81 165 119 202 1.09e-21 94.0
MS.gene039465.t1 AT2G42300 70.000 50 15 0 90 139 175 224 1.44e-18 83.2
MS.gene039465.t1 AT2G42300 70.000 50 15 0 90 139 175 224 1.44e-18 83.2
MS.gene039465.t1 AT2G42300 70.000 50 15 0 90 139 175 224 1.97e-18 82.8
MS.gene039465.t1 AT2G20180 53.125 64 29 1 102 165 280 342 1.21e-14 74.3
MS.gene039465.t1 AT2G20180 53.125 64 29 1 102 165 280 342 1.21e-14 74.3
MS.gene039465.t1 AT2G20180 53.125 64 29 1 102 165 280 342 1.21e-14 74.3
MS.gene039465.t1 AT2G20180 53.125 64 29 1 102 165 280 342 1.21e-14 74.3
MS.gene039465.t1 AT2G20180 53.968 63 28 1 103 165 210 271 1.25e-14 73.9
MS.gene039465.t1 AT2G20180 53.968 63 28 1 103 165 210 271 1.25e-14 73.9
MS.gene039465.t1 AT2G20180 53.968 63 28 1 103 165 210 271 1.25e-14 73.9
MS.gene039465.t1 AT1G09530 46.479 71 37 1 95 165 332 401 1.87e-14 73.9
MS.gene039465.t1 AT1G09530 46.479 71 37 1 95 165 332 401 1.87e-14 73.9
MS.gene039465.t1 AT1G09530 46.479 71 37 1 95 165 332 401 1.87e-14 73.9
MS.gene039465.t1 AT1G09530 46.479 71 37 1 95 165 332 401 1.87e-14 73.9
MS.gene039465.t1 AT1G09530 46.479 71 37 1 95 165 332 401 1.87e-14 73.9
MS.gene039465.t1 AT1G09530 46.479 71 37 1 95 165 332 401 1.87e-14 73.9
MS.gene039465.t1 AT2G42280 36.667 120 72 2 44 163 227 342 3.71e-14 72.4
MS.gene039465.t1 AT4G28790 35.043 117 67 3 102 217 273 381 3.92e-14 72.8
MS.gene039465.t1 AT2G42280 37.705 122 70 3 44 163 227 344 4.18e-14 72.4
MS.gene039465.t1 AT4G09180 50.746 67 33 0 98 164 182 248 4.59e-14 71.2
MS.gene039465.t1 AT1G51140 54.688 64 29 0 100 163 304 367 6.17e-14 72.0
MS.gene039465.t1 AT4G28800 32.667 150 77 5 61 209 224 350 8.57e-14 71.6
MS.gene039465.t1 AT4G02590 51.724 87 33 2 87 165 62 147 1.32e-13 69.7
MS.gene039465.t1 AT4G02590 51.724 87 33 2 87 165 125 210 2.02e-13 70.1
MS.gene039465.t1 AT4G02590 51.724 87 33 2 87 165 125 210 2.02e-13 70.1
MS.gene039465.t1 AT1G03040 62.121 66 24 1 100 165 139 203 2.20e-13 69.7
MS.gene039465.t1 AT1G35460 50.769 65 32 0 100 164 181 245 2.40e-13 68.9
MS.gene039465.t1 AT4G28815 48.571 70 34 2 103 171 71 139 2.61e-13 68.6
MS.gene039465.t1 AT1G03040 62.121 66 24 1 100 165 144 208 2.70e-13 69.3
MS.gene039465.t1 AT2G20180 53.333 60 27 1 102 161 280 338 2.76e-13 69.7
MS.gene039465.t1 AT1G03040 62.121 66 24 1 100 165 144 208 4.35e-13 69.3
MS.gene039465.t1 AT4G28815 48.571 70 34 2 103 171 147 215 6.31e-13 68.6
MS.gene039465.t1 AT1G27740 38.938 113 68 1 52 164 119 230 2.00e-12 66.2
MS.gene039465.t1 AT4G28800 38.835 103 48 3 61 163 236 323 4.53e-12 66.6
MS.gene039465.t1 AT2G46970 41.250 80 41 2 98 172 194 272 5.10e-12 66.2
MS.gene039465.t1 AT2G46970 41.250 80 41 2 98 172 216 294 5.11e-12 66.2
MS.gene039465.t1 AT2G46970 41.250 80 41 2 98 172 185 263 5.20e-12 66.2
MS.gene039465.t1 AT2G14760 39.655 116 56 4 59 164 189 300 1.06e-11 65.1
MS.gene039465.t1 AT4G33880 43.011 93 49 3 75 164 238 329 3.32e-11 63.5
MS.gene039465.t1 AT4G00050 49.231 65 32 1 100 164 105 168 4.72e-11 62.8
MS.gene039465.t1 AT2G43140 48.438 64 33 0 100 163 134 197 6.24e-11 61.2
MS.gene039465.t1 AT4G00050 49.231 65 32 1 100 164 207 270 7.73e-11 62.8
MS.gene039465.t1 AT4G36930 49.180 61 30 1 106 166 197 256 8.50e-11 62.4
MS.gene039465.t1 AT4G00050 49.231 65 32 1 100 164 207 270 8.94e-11 62.4

Find 54 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTTTGCTCAGTGATTT+TGG 0.069577 4.3:+35289829 None:intergenic
CACTTTGATCCTCTTGTCTT+TGG 0.165749 4.3:+35289871 None:intergenic
GGGATAGTCCCTCCGGAAAT+TGG 0.186320 4.3:-35288540 MS.gene039465:CDS
GACATTTCTGATTGCATCAT+TGG 0.242720 4.3:+35288636 None:intergenic
TTCCTTCGAACCCCAATTTC+CGG 0.243538 4.3:+35288528 None:intergenic
AATTTGTATCTACCTGTAAA+TGG 0.288243 4.3:+35288249 None:intergenic
TTTCCTTCTCAACCATTTAC+AGG 0.299320 4.3:-35288261 MS.gene039465:intron
GATGAGTCAAGAAAGATTTC+AGG 0.310824 4.3:+35288477 None:intergenic
GTTGGTTGTGATTCTGCTCT+TGG 0.325858 4.3:-35289992 MS.gene039465:CDS
GCATTGTGTCCATACCTCTT+TGG 0.342272 4.3:+35288912 None:intergenic
TGGTGGATTGATAAACAATA+TGG 0.350380 4.3:-35288562 MS.gene039465:CDS
GGATAGTCCCTCCGGAAATT+GGG 0.350458 4.3:-35288539 MS.gene039465:CDS
AACAAAATTGCAGGCAAAGC+TGG 0.365496 4.3:-35289193 MS.gene039465:CDS
CAATGTTGAAGTCAAGTCTT+GGG 0.403046 4.3:+35288947 None:intergenic
CCAGGATGTAACAAAATTGC+AGG 0.430070 4.3:-35289202 MS.gene039465:CDS
TGATGAATTGTTTGCCAAAG+AGG 0.438799 4.3:-35288926 MS.gene039465:intron
GCTCGAACATGAATGTAATC+AGG 0.466193 4.3:+35289713 None:intergenic
TCAATGTTGAAGTCAAGTCT+TGG 0.479932 4.3:+35288946 None:intergenic
GAATTGAATTGAAGATATGC+AGG 0.482069 4.3:+35288609 None:intergenic
ATCAAAGTGAGTGTTGAAGA+AGG 0.498660 4.3:-35289857 MS.gene039465:CDS
TCGAAGAAACCATTAAGAAA+AGG 0.502237 4.3:-35289922 MS.gene039465:CDS
ATATCCTACCATCCTCAACT+TGG 0.507057 4.3:-35288341 MS.gene039465:CDS
AAAATCACCTTCCCAAGTTG+AGG 0.508180 4.3:+35288329 None:intergenic
CAATATGGGGATAGTCCCTC+CGG 0.510922 4.3:-35288547 MS.gene039465:CDS
TATTTGCAAGATTTAGTACC+AGG 0.510967 4.3:-35289220 MS.gene039465:CDS
GGTGGATTGATAAACAATAT+GGG 0.517497 4.3:-35288561 MS.gene039465:CDS
TAACTCTATGAAACCCGACC+CGG 0.524469 4.3:-35289957 MS.gene039465:CDS
TGAAGCTAGCAATCTGAAGA+TGG 0.529573 4.3:-35287927 MS.gene039465:CDS
GTCTCTTCAAAGACAAGTTG+AGG 0.543289 4.3:-35289140 MS.gene039465:intron
CAACAACTAGTTTCATGTTG+TGG 0.545151 4.3:-35288582 MS.gene039465:CDS
AGAGAAAATTGTGATGATGT+TGG 0.545309 4.3:-35289779 MS.gene039465:CDS
CTCCGGAAATTGGGGTTCGA+AGG 0.548238 4.3:-35288530 MS.gene039465:CDS
CTACCTGTAAATGGTTGAGA+AGG 0.549856 4.3:+35288258 None:intergenic
CTTCCCAAGTTGAGGATGGT+AGG 0.556292 4.3:+35288337 None:intergenic
CAACTAGTTTCATGTTGTGG+TGG 0.563540 4.3:-35288579 MS.gene039465:CDS
AAAATCACTGAGCAAACAAA+GGG 0.564488 4.3:-35289827 MS.gene039465:CDS
GATAGTCCCTCCGGAAATTG+GGG 0.565816 4.3:-35288538 MS.gene039465:CDS
TTGTGATGATGTTGGTTCCA+AGG 0.568124 4.3:-35289771 MS.gene039465:CDS
TCAGGTAAAGATGCAGATGC+CGG 0.578456 4.3:+35288495 None:intergenic
TCGAAGGAACATTAATGCAC+CGG 0.582886 4.3:-35288514 MS.gene039465:CDS
CAAAATCACTGAGCAAACAA+AGG 0.583100 4.3:-35289828 MS.gene039465:CDS
CTACCATCCTCAACTTGGGA+AGG 0.586675 4.3:-35288336 MS.gene039465:CDS
GGTTCCAAGGAAAATTCGAA+AGG 0.600922 4.3:-35289758 MS.gene039465:CDS
TATCCTACCATCCTCAACTT+GGG 0.602359 4.3:-35288340 MS.gene039465:CDS
AATTGAATTGAAGATATGCA+GGG 0.606589 4.3:+35288610 None:intergenic
TCACCTTCCCAAGTTGAGGA+TGG 0.614865 4.3:+35288333 None:intergenic
CTTCGAACCCCAATTTCCGG+AGG 0.622595 4.3:+35288531 None:intergenic
TTCGAACCCCAATTTCCGGA+GGG 0.626102 4.3:+35288532 None:intergenic
AACTCTATGAAACCCGACCC+GGG 0.629719 4.3:-35289956 MS.gene039465:CDS
GAAAAGGAAAGCTGATAACA+AGG 0.638853 4.3:-35289906 MS.gene039465:CDS
ATATAAAGTCCAAAGACAAG+AGG 0.657536 4.3:-35289880 MS.gene039465:CDS
ATTCATGTTCGAGCACGTCG+CGG 0.664373 4.3:-35289704 MS.gene039465:CDS
GTGGATTGATAAACAATATG+GGG 0.684555 4.3:-35288560 MS.gene039465:CDS
AAGGAAAGCTGATAACAAGG+TGG 0.715030 4.3:-35289903 MS.gene039465:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAGTTTAAGTTTATAATA+TGG - chr4.3:35288853-35288872 MS.gene039465:intron 10.0%
!!! TTTTACTAAATTGTTTAATT+TGG - chr4.3:35288471-35288490 MS.gene039465:CDS 10.0%
!! AATCATATTAATGATTGATT+AGG + chr4.3:35289514-35289533 None:intergenic 15.0%
!! TTAAATTATTTGATCTTTAC+AGG - chr4.3:35289238-35289257 MS.gene039465:CDS 15.0%
!!! ATTTTATGGTTTTAGTAAAA+AGG - chr4.3:35289028-35289047 MS.gene039465:intron 15.0%
!! ATGATTGAAAGTTGAAAATT+CGG - chr4.3:35289867-35289886 MS.gene039465:CDS 20.0%
!! ATGCTAAAATTACAGTTAAT+AGG - chr4.3:35288498-35288517 MS.gene039465:CDS 20.0%
!! GTATGATAAATTAGTTACTT+AGG - chr4.3:35288808-35288827 MS.gene039465:intron 20.0%
!! TAGTAAAAAATGATGTAACA+CGG - chr4.3:35288372-35288391 MS.gene039465:intron 20.0%
!!! AAATCTTCATTTTGATGAAA+AGG + chr4.3:35289816-35289835 None:intergenic 20.0%
!!! TCTCTTAATTTGTCATTATT+TGG - chr4.3:35288301-35288320 MS.gene039465:CDS 20.0%
!!! TGATTTTTCTGTTTATTACT+CGG - chr4.3:35288603-35288622 MS.gene039465:CDS 20.0%
! AATTGAATTGAAGATATGCA+GGG + chr4.3:35289307-35289326 None:intergenic 25.0%
! AATTTGTATCTACCTGTAAA+TGG + chr4.3:35289668-35289687 None:intergenic 25.0%
! ACACTTATTATGTGCAATAT+AGG - chr4.3:35288569-35288588 MS.gene039465:CDS 25.0%
! CTAAAATTACAGTTAATAGG+AGG - chr4.3:35288501-35288520 MS.gene039465:CDS 25.0%
!! TTTTCAAAATCTTCATAGTG+TGG - chr4.3:35289604-35289623 MS.gene039465:intron 25.0%
!!! GATTAGGAACAATATTTTAG+AGG + chr4.3:35289498-35289517 None:intergenic 25.0%
AAAATCACTGAGCAAACAAA+GGG - chr4.3:35288087-35288106 MS.gene039465:intron 30.0%
AAAGGACTTATCAATATCTG+TGG + chr4.3:35289798-35289817 None:intergenic 30.0%
AAGGACTTATCAATATCTGT+GGG + chr4.3:35289797-35289816 None:intergenic 30.0%
ATATAAAGTCCAAAGACAAG+AGG - chr4.3:35288034-35288053 MS.gene039465:intron 30.0%
GAATTGAATTGAAGATATGC+AGG + chr4.3:35289308-35289327 None:intergenic 30.0%
GTTTATTACTCGGAGTTTAA+AGG - chr4.3:35288613-35288632 MS.gene039465:CDS 30.0%
TATTTGCAAGATTTAGTACC+AGG - chr4.3:35288694-35288713 MS.gene039465:intron 30.0%
TCGAAGAAACCATTAAGAAA+AGG - chr4.3:35287992-35288011 MS.gene039465:intron 30.0%
TCTACATTTGACCGTAATTA+AGG - chr4.3:35289111-35289130 MS.gene039465:intron 30.0%
! AGAGAAAATTGTGATGATGT+TGG - chr4.3:35288135-35288154 MS.gene039465:intron 30.0%
! ATCAGCTTTCCTTTTCTTAA+TGG + chr4.3:35288004-35288023 None:intergenic 30.0%
! CAAAAAGCGCAAATCATTAA+TGG + chr4.3:35289193-35289212 None:intergenic 30.0%
! GGACACAATGCAAAATTTTA+TGG - chr4.3:35289014-35289033 MS.gene039465:intron 30.0%
! GTGGATTGATAAACAATATG+GGG - chr4.3:35289354-35289373 MS.gene039465:intron 30.0%
!! AGGAACAATATTTTAGAGGA+CGG + chr4.3:35289494-35289513 None:intergenic 30.0%
!! GGTGGATTGATAAACAATAT+GGG - chr4.3:35289353-35289372 MS.gene039465:intron 30.0%
!! TGGTGGATTGATAAACAATA+TGG - chr4.3:35289352-35289371 MS.gene039465:intron 30.0%
!!! ATGTTTTGTTTTATGACTGC+AGG - chr4.3:35289958-35289977 MS.gene039465:CDS 30.0%
AATTGTTTGCCAAAGAGGTA+TGG - chr4.3:35288993-35289012 MS.gene039465:intron 35.0%
AGATGATACAGCCTTAATCA+CGG + chr4.3:35289094-35289113 None:intergenic 35.0%
AGGACTTATCAATATCTGTG+GGG + chr4.3:35289796-35289815 None:intergenic 35.0%
CAAAATCACTGAGCAAACAA+AGG - chr4.3:35288086-35288105 MS.gene039465:intron 35.0%
CAATGTTGAAGTCAAGTCTT+GGG + chr4.3:35288970-35288989 None:intergenic 35.0%
CCTTGCAAACCTTTATATTC+CGG - chr4.3:35289899-35289918 MS.gene039465:CDS 35.0%
CTTTATATTCCGGAAAAACG+CGG - chr4.3:35289909-35289928 MS.gene039465:CDS 35.0%
GAAAAGGAAAGCTGATAACA+AGG - chr4.3:35288008-35288027 MS.gene039465:intron 35.0%
GAAGGGAAAAAATAAGCGAA+AGG - chr4.3:35288665-35288684 MS.gene039465:intron 35.0%
GACATTTCTGATTGCATCAT+TGG + chr4.3:35289281-35289300 None:intergenic 35.0%
GACTCATAACGCCTTAATTA+CGG + chr4.3:35289125-35289144 None:intergenic 35.0%
GATGAGTCAAGAAAGATTTC+AGG + chr4.3:35289440-35289459 None:intergenic 35.0%
TATCCATCAATCATGATCGT+CGG + chr4.3:35288411-35288430 None:intergenic 35.0%
TCAATGTTGAAGTCAAGTCT+TGG + chr4.3:35288971-35288990 None:intergenic 35.0%
TCATTGACAAGAACTGCATT+TGG + chr4.3:35288931-35288950 None:intergenic 35.0%
TCTCAAGTCTCTATCATCTA+TGG + chr4.3:35289772-35289791 None:intergenic 35.0%
TGATGAATTGTTTGCCAAAG+AGG - chr4.3:35288988-35289007 MS.gene039465:intron 35.0%
TTTCCTTCTCAACCATTTAC+AGG - chr4.3:35289653-35289672 MS.gene039465:intron 35.0%
! ATGTGCAATATAGGTTAGCT+CGG - chr4.3:35288578-35288597 MS.gene039465:CDS 35.0%
! CAACAACTAGTTTCATGTTG+TGG - chr4.3:35289332-35289351 MS.gene039465:intron 35.0%
! TGAACCTTTCGAATTTTCCT+TGG + chr4.3:35288163-35288182 None:intergenic 35.0%
!! ATCAAAGTGAGTGTTGAAGA+AGG - chr4.3:35288057-35288076 MS.gene039465:intron 35.0%
!! CATAGTGTGGATTTTGATCA+AGG - chr4.3:35289617-35289636 MS.gene039465:intron 35.0%
!! CTTTCTACTTCTAAGATTGC+AGG + chr4.3:35289721-35289740 None:intergenic 35.0%
!! CTTTGTTTGCTCAGTGATTT+TGG + chr4.3:35288088-35288107 None:intergenic 35.0%
AAAATCACCTTCCCAAGTTG+AGG + chr4.3:35289588-35289607 None:intergenic 40.0%
AAAGGTTTGCAAGGTAACCA+CGG + chr4.3:35289893-35289912 None:intergenic 40.0%
AACAAAATTGCAGGCAAAGC+TGG - chr4.3:35288721-35288740 MS.gene039465:intron 40.0%
AAGGAAAGCTGATAACAAGG+TGG - chr4.3:35288011-35288030 MS.gene039465:intron 40.0%
ATATCCTACCATCCTCAACT+TGG - chr4.3:35289573-35289592 MS.gene039465:intron 40.0%
ATTCCGACGATCATGATTGA+TGG - chr4.3:35288405-35288424 MS.gene039465:intron 40.0%
CACTTTGATCCTCTTGTCTT+TGG + chr4.3:35288046-35288065 None:intergenic 40.0%
CCAGGATGTAACAAAATTGC+AGG - chr4.3:35288712-35288731 MS.gene039465:intron 40.0%
CCGGAATATAAAGGTTTGCA+AGG + chr4.3:35289902-35289921 None:intergenic 40.0%
CTACCTGTAAATGGTTGAGA+AGG + chr4.3:35289659-35289678 None:intergenic 40.0%
GCACAAAACAGAACAAACGT+TGG + chr4.3:35288639-35288658 None:intergenic 40.0%
GCTCGAACATGAATGTAATC+AGG + chr4.3:35288204-35288223 None:intergenic 40.0%
GGCATCAAAAATCATGATGC+TGG + chr4.3:35289061-35289080 None:intergenic 40.0%
GGTTCCAAGGAAAATTCGAA+AGG - chr4.3:35288156-35288175 MS.gene039465:intron 40.0%
GTCTCTTCAAAGACAAGTTG+AGG - chr4.3:35288774-35288793 MS.gene039465:intron 40.0%
TATCCTACCATCCTCAACTT+GGG - chr4.3:35289574-35289593 MS.gene039465:intron 40.0%
TCGAAGGAACATTAATGCAC+CGG - chr4.3:35289400-35289419 MS.gene039465:intron 40.0%
TGAAGCTAGCAATCTGAAGA+TGG - chr4.3:35289987-35290006 MS.gene039465:CDS 40.0%
TGTTGTGATTCGCGATATTG+TGG + chr4.3:35289167-35289186 None:intergenic 40.0%
! ACGTTTGTTCTGTTTTGTGC+AGG - chr4.3:35288639-35288658 MS.gene039465:CDS 40.0%
! CAACTAGTTTCATGTTGTGG+TGG - chr4.3:35289335-35289354 MS.gene039465:intron 40.0%
! CCTGCAATTTTGTTACATCC+TGG + chr4.3:35288715-35288734 None:intergenic 40.0%
! CGCGTTTTTCCGGAATATAA+AGG + chr4.3:35289911-35289930 None:intergenic 40.0%
! CTGTTTTGTGCAGGTTAGAA+GGG - chr4.3:35288648-35288667 MS.gene039465:CDS 40.0%
! GCATCATGATTTTTGATGCC+AGG - chr4.3:35289061-35289080 MS.gene039465:intron 40.0%
! TCTGTTTTGTGCAGGTTAGA+AGG - chr4.3:35288647-35288666 MS.gene039465:CDS 40.0%
!! ACTTCTAAGATTGCAGGCTA+AGG + chr4.3:35289715-35289734 None:intergenic 40.0%
!! GGTTTTAGTAAAAAGGTCCG+CGG - chr4.3:35289035-35289054 MS.gene039465:intron 40.0%
!! TTGTGATGATGTTGGTTCCA+AGG - chr4.3:35288143-35288162 MS.gene039465:intron 40.0%
AAAAATCATGATGCTGGCCG+CGG + chr4.3:35289055-35289074 None:intergenic 45.0%
AAAACGCGGTAGATTCGATG+CGG - chr4.3:35289923-35289942 MS.gene039465:CDS 45.0%
CGCGATATTGTGGATAATCG+CGG + chr4.3:35289157-35289176 None:intergenic 45.0%
GAAAGTTGAAAATTCGGCCG+TGG - chr4.3:35289873-35289892 MS.gene039465:CDS 45.0%
GCATTGTGTCCATACCTCTT+TGG + chr4.3:35289005-35289024 None:intergenic 45.0%
GTTGGTTGTGATTCTGCTCT+TGG - chr4.3:35287922-35287941 MS.gene039465:CDS 45.0%
TAACTCTATGAAACCCGACC+CGG - chr4.3:35287957-35287976 MS.gene039465:intron 45.0%
TCAGGTAAAGATGCAGATGC+CGG + chr4.3:35289422-35289441 None:intergenic 45.0%
TTCCTTCGAACCCCAATTTC+CGG + chr4.3:35289389-35289408 None:intergenic 45.0%
! TCGAATCTACCGCGTTTTTC+CGG + chr4.3:35289921-35289940 None:intergenic 45.0%
! TCTTCGACGTTTTCGAAACC+CGG + chr4.3:35287979-35287998 None:intergenic 45.0%
AAACGCGGTAGATTCGATGC+GGG - chr4.3:35289924-35289943 MS.gene039465:CDS 50.0%
AACTCTATGAAACCCGACCC+GGG - chr4.3:35287958-35287977 MS.gene039465:intron 50.0%
ATTCATGTTCGAGCACGTCG+CGG - chr4.3:35288210-35288229 MS.gene039465:intron 50.0%
CAATATGGGGATAGTCCCTC+CGG - chr4.3:35289367-35289386 MS.gene039465:intron 50.0%
CTACCATCCTCAACTTGGGA+AGG - chr4.3:35289578-35289597 MS.gene039465:intron 50.0%
GATAGTCCCTCCGGAAATTG+GGG - chr4.3:35289376-35289395 MS.gene039465:intron 50.0%
GGATAGTCCCTCCGGAAATT+GGG - chr4.3:35289375-35289394 MS.gene039465:intron 50.0%
TACAGCCTTAATCACGGTCG+TGG + chr4.3:35289088-35289107 None:intergenic 50.0%
TAGAGGACGGATACACAGAG+AGG + chr4.3:35289481-35289500 None:intergenic 50.0%
TTCGAACCCCAATTTCCGGA+GGG + chr4.3:35289385-35289404 None:intergenic 50.0%
! CTTCCCAAGTTGAGGATGGT+AGG + chr4.3:35289580-35289599 None:intergenic 50.0%
! CTTCGACGTTTTCGAAACCC+GGG + chr4.3:35287978-35287997 None:intergenic 50.0%
! TCACCTTCCCAAGTTGAGGA+TGG + chr4.3:35289584-35289603 None:intergenic 50.0%
CAGGTCCACGACCGTGATTA+AGG - chr4.3:35289080-35289099 MS.gene039465:intron 55.0%
CTCCGGAAATTGGGGTTCGA+AGG - chr4.3:35289384-35289403 MS.gene039465:intron 55.0%
CTTAATCACGGTCGTGGACC+TGG + chr4.3:35289082-35289101 None:intergenic 55.0%
CTTCGAACCCCAATTTCCGG+AGG + chr4.3:35289386-35289405 None:intergenic 55.0%
GGGATAGTCCCTCCGGAAAT+TGG - chr4.3:35289374-35289393 MS.gene039465:intron 55.0%
! ACGTTTTCGAAACCCGGGTC+GGG + chr4.3:35287973-35287992 None:intergenic 55.0%
! GACGTTTTCGAAACCCGGGT+CGG + chr4.3:35287974-35287993 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 35287919 35290017 35287919 ID=MS.gene039465
chr4.3 mRNA 35287919 35290017 35287919 ID=MS.gene039465.t1;Parent=MS.gene039465
chr4.3 exon 35289670 35290017 35289670 ID=MS.gene039465.t1.exon1;Parent=MS.gene039465.t1
chr4.3 CDS 35289670 35290017 35289670 ID=cds.MS.gene039465.t1;Parent=MS.gene039465.t1
chr4.3 exon 35289141 35289275 35289141 ID=MS.gene039465.t1.exon2;Parent=MS.gene039465.t1
chr4.3 CDS 35289141 35289275 35289141 ID=cds.MS.gene039465.t1;Parent=MS.gene039465.t1
chr4.3 exon 35288927 35288998 35288927 ID=MS.gene039465.t1.exon3;Parent=MS.gene039465.t1
chr4.3 CDS 35288927 35288998 35288927 ID=cds.MS.gene039465.t1;Parent=MS.gene039465.t1
chr4.3 exon 35288467 35288676 35288467 ID=MS.gene039465.t1.exon4;Parent=MS.gene039465.t1
chr4.3 CDS 35288467 35288676 35288467 ID=cds.MS.gene039465.t1;Parent=MS.gene039465.t1
chr4.3 exon 35288262 35288364 35288262 ID=MS.gene039465.t1.exon5;Parent=MS.gene039465.t1
chr4.3 CDS 35288262 35288364 35288262 ID=cds.MS.gene039465.t1;Parent=MS.gene039465.t1
chr4.3 exon 35287919 35287956 35287919 ID=MS.gene039465.t1.exon6;Parent=MS.gene039465.t1
chr4.3 CDS 35287919 35287956 35287919 ID=cds.MS.gene039465.t1;Parent=MS.gene039465.t1
Gene Sequence

>MS.gene039465

ATGGTTGGTTGTGATTCTGCTCTTGGTGAAGTTGTAGCTAACTCTATGAAACCCGACCCGGGTTTCGAAAACGTCGAAGAAACCATTAAGAAAAGGAAAGCTGATAACAAGGTGGATATAAAGTCCAAAGACAAGAGGATCAAAGTGAGTGTTGAAGAAGGTGAATCCAAAATCACTGAGCAAACAAAGGGACAGAAGAATACAAAGTTGAAAAATAGAGAAAATTGTGATGATGTTGGTTCCAAGGAAAATTCGAAAGGTTCAGAGATTCAAAATCAAAAGCCTGATTACATTCATGTTCGAGCACGTCGCGGTCAAGCTACTGATAGTCATAGCTTAGCTGAAAGAGTGAGTATAAATTAACAATTACTTTCACTATTTTTCTCTTAATTTGTCATTATTTGGTTAATAAAAAGTGATTATCAATAAACTCATTTTACACAATTGATTTGATAGTAAAAAATGATGTAACACGGTGAAGAAGTTATTCCGACGATCATGATTGATGGATAGTGTAAAAACATTTTACTTATAACAATTAACTCATTTATATTTTACTAAATTGTTTAATTTGGTTTAATGCTAAAATTACAGTTAATAGGAGGTAGAATCTGTTTTAAAAACATAGTTTCTATAATGAAATCAAATAGACACTTATTATGTGCAATATAGGTTAGCTCGGTATGATTTTTCTGTTTATTACTCGGAGTTTAAAGGCCAACGTTTGTTCTGTTTTGTGCAGGTTAGAAGGGAAAAAATAAGCGAAAGGATGAAGTATTTGCAAGATTTAGTACCAGGATGTAACAAAATTGCAGGCAAAGCTGGAATGCTTGATGAAATTATTAACTATGTTCAGTCTCTTCAAAGACAAGTTGAGGTTAGAGTTCTAGTATGATAAATTAGTTACTTAGGCTGCATGAATTATAGTGTTTGATTAAGTTTAAGTTTATAATATGGTTTCAATTTTAAGAATAATTTAAAGTCTATCTAACTAATTTGTACATTATCACCAAATGCAGTTCTTGTCAATGAAATTAGCAGCTGTAAACCCAAGACTTGACTTCAACATTGATGAATTGTTTGCCAAAGAGGTATGGACACAATGCAAAATTTTATGGTTTTAGTAAAAAGGTCCGCGGCCAGCATCATGATTTTTGATGCCAGGTCCACGACCGTGATTAAGGCTGTATCATCTACATTTGACCGTAATTAAGGCGTTATGAGTCAACATTTGACCGCGATTATCCACAATATCGCGAATCACAACACAGCCATTAATGATTTGCGCTTTTTGTTTTAGAATGCATGCTGAAGTGCTCTTAAATTATTTGATCTTTACAGGTGTTTACTCAAAATTTTCCAATGATGCAATCAGAAATGTCTAACCCTGCATATCTTCAATTCAATTCAACACAACAACTAGTTTCATGTTGTGGTGGATTGATAAACAATATGGGGATAGTCCCTCCGGAAATTGGGGTTCGAAGGAACATTAATGCACCGGCATCTGCATCTTTACCTGAAATCTTTCTTGACTCATCATGTTTCACTGTATGTTTCCTCTCTGTGTATCCGTCCTCTAAAATATTGTTCCTAATCAATCATTAATATGATTAGTCTTCTACAATTGATCTTAATTTATGTGTATTGCAGCATATCCTACCATCCTCAACTTGGGAAGGTGATTTTCAAAATCTTCATAGTGTGGATTTTGATCAAGGAAGATCAACATCATTTCCTTCTCAACCATTTACAGGTAGATACAAATTTATGACATGTCAAATCACTTTTTGCCTTAGCCTGCAATCTTAGAAGTAGAAAGTAGAAGCAAGACAAATTGAATTTTCATACCATAGATGATAGAGACTTGAGAGCCCCACAGATATTGATAAGTCCTTTTCATCAAAATGAAGATTTTTTATTTCATTACTTTTCTTTTTATCTGCTAATGATTGAAAGTTGAAAATTCGGCCGTGGTTACCTTGCAAACCTTTATATTCCGGAAAAACGCGGTAGATTCGATGCGGGAACACTTCTAAATGTTTTGTTTTATGACTGCAGGCATGATTGAAGCTAGCAATCTGAAGATGGAGATGTAA
Protein sequence

>MS.gene039465.t1

MVGCDSALGEVVANSMKPDPGFENVEETIKKRKADNKVDIKSKDKRIKVSVEEGESKITEQTKGQKNTKLKNRENCDDVGSKENSKGSEIQNQKPDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKIAGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNIDELFAKEVFTQNFPMMQSEMSNPAYLQFNSTQQLVSCCGGLINNMGIVPPEIGVRRNINAPASASLPEIFLDSSCFTHILPSSTWEGDFQNLHSVDFDQGRSTSFPSQPFTGMIEASNLKMEM