Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039667.t1 | RHN65706.1 | 96.4 | 84 | 3 | 0 | 1 | 84 | 1 | 84 | 5.00E-37 | 163.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039667.t1 | Q4GZK2 | 67.9 | 84 | 21 | 2 | 2 | 84 | 7 | 85 | 6.6e-22 | 104.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039667.t1 | G7IVX4 | 96.4 | 84 | 3 | 0 | 1 | 84 | 1 | 84 | 3.6e-37 | 163.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene039667.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039667.t1 | MTR_3g015490 | 95.294 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 1.78e-56 | 171 |
| MS.gene039667.t1 | MTR_8g038620 | 75.904 | 83 | 19 | 1 | 3 | 85 | 8 | 89 | 7.21e-40 | 129 |
| MS.gene039667.t1 | MTR_7g490310 | 66.667 | 84 | 28 | 0 | 2 | 85 | 7 | 90 | 1.12e-36 | 123 |
| MS.gene039667.t1 | MTR_6g007460 | 71.084 | 83 | 15 | 1 | 3 | 85 | 10 | 83 | 1.02e-35 | 120 |
| MS.gene039667.t1 | MTR_3g088630 | 56.989 | 93 | 27 | 2 | 4 | 85 | 22 | 112 | 2.41e-28 | 102 |
| MS.gene039667.t1 | MTR_5g036480 | 53.012 | 83 | 34 | 1 | 3 | 85 | 15 | 92 | 1.66e-24 | 92.4 |
| MS.gene039667.t1 | MTR_3g093860 | 51.163 | 86 | 26 | 2 | 5 | 85 | 19 | 93 | 3.16e-23 | 87.0 |
| MS.gene039667.t1 | MTR_1g049100 | 55.952 | 84 | 28 | 3 | 3 | 85 | 7 | 82 | 4.33e-23 | 87.8 |
| MS.gene039667.t1 | MTR_3g078613 | 51.765 | 85 | 32 | 2 | 1 | 85 | 20 | 95 | 1.10e-21 | 84.0 |
| MS.gene039667.t1 | MTR_3g078613 | 51.765 | 85 | 32 | 2 | 1 | 85 | 20 | 95 | 1.15e-21 | 84.0 |
| MS.gene039667.t1 | MTR_4g106590 | 52.381 | 84 | 31 | 2 | 2 | 85 | 24 | 98 | 3.16e-21 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039667.t1 | AT3G57040 | 65.116 | 86 | 27 | 2 | 2 | 85 | 7 | 91 | 4.81e-31 | 108 |
| MS.gene039667.t1 | AT3G57040 | 65.116 | 86 | 27 | 2 | 2 | 85 | 7 | 91 | 4.81e-31 | 108 |
| MS.gene039667.t1 | AT2G41310 | 59.524 | 84 | 33 | 1 | 2 | 85 | 7 | 89 | 2.73e-29 | 104 |
| MS.gene039667.t1 | AT1G59940 | 56.627 | 83 | 27 | 2 | 3 | 85 | 32 | 105 | 7.87e-25 | 92.8 |
| MS.gene039667.t1 | AT1G10470 | 56.471 | 85 | 28 | 3 | 1 | 85 | 17 | 92 | 3.57e-24 | 91.7 |
| MS.gene039667.t1 | AT1G10470 | 57.831 | 83 | 26 | 3 | 3 | 85 | 33 | 106 | 5.06e-24 | 91.7 |
| MS.gene039667.t1 | AT1G59940 | 56.627 | 83 | 27 | 2 | 3 | 85 | 124 | 197 | 5.81e-24 | 92.4 |
| MS.gene039667.t1 | AT3G56380 | 51.807 | 83 | 32 | 2 | 3 | 85 | 19 | 93 | 1.13e-23 | 88.2 |
| MS.gene039667.t1 | AT3G56380 | 51.807 | 83 | 32 | 2 | 3 | 85 | 19 | 93 | 1.16e-23 | 88.2 |
| MS.gene039667.t1 | AT2G40670 | 50.602 | 83 | 35 | 1 | 3 | 85 | 28 | 104 | 1.87e-22 | 85.1 |
| MS.gene039667.t1 | AT2G40670 | 50.602 | 83 | 35 | 1 | 3 | 85 | 28 | 104 | 1.94e-22 | 85.1 |
| MS.gene039667.t1 | AT3G48100 | 52.439 | 82 | 31 | 2 | 4 | 85 | 25 | 98 | 3.80e-22 | 85.1 |
| MS.gene039667.t1 | AT1G19050 | 49.398 | 83 | 33 | 1 | 3 | 85 | 23 | 96 | 5.88e-22 | 85.1 |
| MS.gene039667.t1 | AT5G62920 | 53.659 | 82 | 29 | 2 | 4 | 85 | 25 | 97 | 1.18e-21 | 83.6 |
| MS.gene039667.t1 | AT1G74890 | 49.398 | 83 | 33 | 1 | 3 | 85 | 17 | 90 | 2.20e-21 | 83.6 |
Find 15 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGGCACAGTTTCATATTT+TGG | 0.112277 | 3.3:+9928923 | None:intergenic |
| TCAGTTACTGTGGTTGATTC+TGG | 0.242120 | 3.3:+9929476 | MS.gene039667:intron |
| CTCAAAACCTCTTCATTTCA+TGG | 0.264136 | 3.3:+9928990 | MS.gene039667:CDS |
| GGCTTTGAAGTTTCTTGGTT+TGG | 0.274386 | 3.3:+9929505 | MS.gene039667:CDS |
| ACTAAGGCTTTGAAGTTTCT+TGG | 0.296459 | 3.3:+9929500 | MS.gene039667:CDS |
| TGTGGTTGATTCTGGTACTA+AGG | 0.342411 | 3.3:+9929484 | MS.gene039667:CDS |
| AAATCATAGCCTGTCATTCC+TGG | 0.381613 | 3.3:-9929742 | None:intergenic |
| GTTTCTGCAGCAATAGTTTG+AGG | 0.420290 | 3.3:-9929554 | None:intergenic |
| TACTGTATGCCAGGAATGAC+AGG | 0.454246 | 3.3:+9929733 | MS.gene039667:CDS |
| ATAACTGATTACTGTATGCC+AGG | 0.518630 | 3.3:+9929724 | MS.gene039667:CDS |
| TTGACAGAATGTTGATTGAA+AGG | 0.523765 | 3.3:+9928964 | MS.gene039667:CDS |
| AAAATTACCATGAAATGAAG+AGG | 0.534519 | 3.3:-9928997 | None:intergenic |
| TATTGCTGCAGAAACAGATC+AGG | 0.546049 | 3.3:+9929562 | MS.gene039667:CDS |
| TCTTGGTTTGGTTGAAGATG+AGG | 0.555035 | 3.3:+9929517 | MS.gene039667:CDS |
| GTTTGGTTGAAGATGAGGTG+AGG | 0.669904 | 3.3:+9929522 | MS.gene039667:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTTATTAATGTTAAAAATT+TGG | + | chr3.3:9929637-9929656 | MS.gene039667:intron | 10.0% |
| !!! | CAAATTTTTAACATTAATAA+AGG | - | chr3.3:9929639-9929658 | None:intergenic | 10.0% |
| !!! | CTTAAAAATTTTAGAAAATA+TGG | + | chr3.3:9929196-9929215 | MS.gene039667:intron | 10.0% |
| !! | ACAAAAATAATATGATTATG+TGG | + | chr3.3:9929251-9929270 | MS.gene039667:intron | 15.0% |
| !!! | AATTTTGAACGATTTTTTTT+TGG | + | chr3.3:9929296-9929315 | MS.gene039667:intron | 15.0% |
| !!! | ATTCAAATAATTTTGTGAAT+TGG | + | chr3.3:9929075-9929094 | MS.gene039667:intron | 15.0% |
| !!! | TATTTGAATTTTGATGTAAA+AGG | - | chr3.3:9929064-9929083 | None:intergenic | 15.0% |
| !! | AAATGATTAGACTAATACAT+AGG | + | chr3.3:9929600-9929619 | MS.gene039667:intron | 20.0% |
| !! | AATGATTAGACTAATACATA+GGG | + | chr3.3:9929601-9929620 | MS.gene039667:intron | 20.0% |
| !! | GGTAATATGTAATGTTTATT+TGG | + | chr3.3:9929317-9929336 | MS.gene039667:intron | 20.0% |
| !!! | AATTTTGTAAAGAACAAAAC+TGG | - | chr3.3:9929150-9929169 | None:intergenic | 20.0% |
| !!! | ATGTATTAGTCTAATCATTT+AGG | - | chr3.3:9929600-9929619 | None:intergenic | 20.0% |
| !!! | TTTTCTCTCATATTTTTTAG+AGG | + | chr3.3:9929019-9929038 | MS.gene039667:intron | 20.0% |
| ! | AAAATTACCATGAAATGAAG+AGG | - | chr3.3:9929000-9929019 | None:intergenic | 25.0% |
| ! | GCAAAAAACAATTAACCAAA+AGG | - | chr3.3:9929681-9929700 | None:intergenic | 25.0% |
| !! | TTTTGTAATTTCAGTTACTG+TGG | + | chr3.3:9929466-9929485 | MS.gene039667:intron | 25.0% |
| !!! | ATATGATTATGTGGTTTGTT+TGG | + | chr3.3:9929260-9929279 | MS.gene039667:intron | 25.0% |
| !!! | TTTGGTTAATTGTTTTTTGC+AGG | + | chr3.3:9929681-9929700 | MS.gene039667:intron | 25.0% |
| TGATGTTCCAAGTTATTACT+TGG | + | chr3.3:9929364-9929383 | MS.gene039667:intron | 30.0% | |
| ! | TTGACAGAATGTTGATTGAA+AGG | + | chr3.3:9928964-9928983 | MS.gene039667:CDS | 30.0% |
| !! | GATGATGATGTTAATCCTTT+TGG | + | chr3.3:9929663-9929682 | MS.gene039667:intron | 30.0% |
| AACTTGGAACATCATCTCTA+AGG | - | chr3.3:9929358-9929377 | None:intergenic | 35.0% | |
| ATAACTGATTACTGTATGCC+AGG | + | chr3.3:9929724-9929743 | MS.gene039667:CDS | 35.0% | |
| CTCAAAACCTCTTCATTTCA+TGG | + | chr3.3:9928990-9929009 | MS.gene039667:CDS | 35.0% | |
| GTGTGTTCCAAGTAATAACT+TGG | - | chr3.3:9929374-9929393 | None:intergenic | 35.0% | |
| ! | ATCATCTCTAAGGATTTCCA+CGG | - | chr3.3:9929348-9929367 | None:intergenic | 35.0% |
| !! | ACTAAGGCTTTGAAGTTTCT+TGG | + | chr3.3:9929500-9929519 | MS.gene039667:CDS | 35.0% |
| AAATCATAGCCTGTCATTCC+TGG | - | chr3.3:9929745-9929764 | None:intergenic | 40.0% | |
| GTTTCTGCAGCAATAGTTTG+AGG | - | chr3.3:9929557-9929576 | None:intergenic | 40.0% | |
| TATTGCTGCAGAAACAGATC+AGG | + | chr3.3:9929562-9929581 | MS.gene039667:CDS | 40.0% | |
| TGGAACACACTTCATACAGA+TGG | + | chr3.3:9929384-9929403 | MS.gene039667:intron | 40.0% | |
| ! | TCAGTTACTGTGGTTGATTC+TGG | + | chr3.3:9929476-9929495 | MS.gene039667:intron | 40.0% |
| ! | TGTGGTTGATTCTGGTACTA+AGG | + | chr3.3:9929484-9929503 | MS.gene039667:CDS | 40.0% |
| !! | GGCTTTGAAGTTTCTTGGTT+TGG | + | chr3.3:9929505-9929524 | MS.gene039667:CDS | 40.0% |
| !! | TCTTGGTTTGGTTGAAGATG+AGG | + | chr3.3:9929517-9929536 | MS.gene039667:CDS | 40.0% |
| GTTTGGTTGAAGATGAGGTG+AGG | + | chr3.3:9929522-9929541 | MS.gene039667:CDS | 45.0% | |
| TACTGTATGCCAGGAATGAC+AGG | + | chr3.3:9929733-9929752 | MS.gene039667:CDS | 45.0% | |
| ! | ATCTCTAAGGATTTCCACGG+CGG | - | chr3.3:9929345-9929364 | None:intergenic | 45.0% |
| ! | ATGTTTATTTGGAACCGCCG+TGG | + | chr3.3:9929328-9929347 | MS.gene039667:intron | 45.0% |
| !!! | AAATTTTTAACATTAATAAA+GGG | - | chr3.3:9929638-9929657 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 9928924 | 9929771 | 9928924 | ID=MS.gene039667 |
| chr3.3 | mRNA | 9928924 | 9929771 | 9928924 | ID=MS.gene039667.t1;Parent=MS.gene039667 |
| chr3.3 | exon | 9928924 | 9929011 | 9928924 | ID=MS.gene039667.t1.exon1;Parent=MS.gene039667.t1 |
| chr3.3 | CDS | 9928924 | 9929011 | 9928924 | ID=cds.MS.gene039667.t1;Parent=MS.gene039667.t1 |
| chr3.3 | exon | 9929480 | 9929583 | 9929480 | ID=MS.gene039667.t1.exon2;Parent=MS.gene039667.t1 |
| chr3.3 | CDS | 9929480 | 9929583 | 9929480 | ID=cds.MS.gene039667.t1;Parent=MS.gene039667.t1 |
| chr3.3 | exon | 9929703 | 9929771 | 9929703 | ID=MS.gene039667.t1.exon3;Parent=MS.gene039667.t1 |
| chr3.3 | CDS | 9929703 | 9929771 | 9929703 | ID=cds.MS.gene039667.t1;Parent=MS.gene039667.t1 |
| Gene Sequence |
| Protein sequence |
