Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039963.t1 | XP_024637264.1 | 85.1 | 154 | 23 | 0 | 10 | 163 | 11 | 164 | 1.10E-74 | 289.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039963.t1 | G7JQ00 | 85.1 | 154 | 23 | 0 | 10 | 163 | 71 | 224 | 8.2e-75 | 289.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene039963.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039963.t1 | MTR_4g123470 | 85.065 | 154 | 23 | 0 | 10 | 163 | 71 | 224 | 2.64e-97 | 280 |
| MS.gene039963.t1 | MTR_7g113060 | 81.579 | 38 | 7 | 0 | 126 | 163 | 3 | 40 | 2.29e-16 | 73.2 |
| MS.gene039963.t1 | MTR_7g025150 | 26.531 | 147 | 108 | 0 | 3 | 149 | 61 | 207 | 3.56e-12 | 62.4 |
| MS.gene039963.t1 | MTR_4g085790 | 27.815 | 151 | 101 | 3 | 3 | 149 | 61 | 207 | 3.56e-12 | 62.4 |
| MS.gene039963.t1 | MTR_1g054495 | 29.655 | 145 | 92 | 5 | 4 | 143 | 62 | 201 | 3.81e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGACTACCTCATACTTT+TGG | 0.338565 | 3.1:+59197593 | MS.gene039963:intron |
| CGATTGTTCTACGAGTTTAG+TGG | 0.409935 | 3.1:+59197306 | MS.gene039963:CDS |
| GTCATGTGTTTATCAACTCT+TGG | 0.410006 | 3.1:-59197831 | None:intergenic |
| ACCTTTGAGGTTCCTATCGT+TGG | 0.413727 | 3.1:+59197456 | MS.gene039963:CDS |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 3.1:-59197708 | None:intergenic |
| ATCTACTTCCACCATGGTAC+TGG | 0.424919 | 3.1:-59197347 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 3.1:-59197707 | None:intergenic |
| AAGAATTTGCCAAAAGTATG+AGG | 0.469320 | 3.1:-59197602 | None:intergenic |
| TGCCGCACTCATTGAATGAT+AGG | 0.473193 | 3.1:-59197418 | None:intergenic |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 3.1:+59197713 | MS.gene039963:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 3.1:+59197707 | MS.gene039963:CDS |
| TTGTGTTGCCAATGGTATTG+AGG | 0.514824 | 3.1:+59197748 | MS.gene039963:CDS |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 3.1:-59197706 | None:intergenic |
| TCTGTATATTTGAGTCTCAC+AGG | 0.555272 | 3.1:-59197680 | None:intergenic |
| GTCTCCGGCCTCAATACCAT+TGG | 0.575295 | 3.1:-59197756 | None:intergenic |
| CTCATAACGTCGTTGACAGA+TGG | 0.587552 | 3.1:+59197270 | MS.gene039963:CDS |
| TTTCAAGTGCAAGTGTGTCA+TGG | 0.589305 | 3.1:+59197787 | MS.gene039963:CDS |
| TAACGTCGTTGACAGATGGT+TGG | 0.592218 | 3.1:+59197274 | MS.gene039963:CDS |
| TGACACTTGCTAGATGGTGA+TGG | 0.594754 | 3.1:+59197242 | None:intergenic |
| GTTGCCAATGGTATTGAGGC+CGG | 0.597504 | 3.1:+59197752 | MS.gene039963:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 3.1:+59197717 | MS.gene039963:CDS |
| GACACTTGCTAGATGGTGAT+GGG | 0.615365 | 3.1:+59197243 | None:intergenic |
| GCCGCACTCATTGAATGATA+GGG | 0.616026 | 3.1:-59197417 | None:intergenic |
| TCGATCGACGAAATCACCTA+AGG | 0.628108 | 3.1:+59197482 | MS.gene039963:CDS |
| ACCCTATCATTCAATGAGTG+CGG | 0.636716 | 3.1:+59197416 | MS.gene039963:CDS |
| GTCGATCGATATCCAACGAT+AGG | 0.637968 | 3.1:-59197468 | None:intergenic |
| CACGATTACGTAATGTTGTG+CGG | 0.649204 | 3.1:+59197638 | MS.gene039963:CDS |
| GTCGCTTTCCAGTACCATGG+TGG | 0.664216 | 3.1:+59197339 | MS.gene039963:CDS |
| GCACTCATTGAATGATAGGG+TGG | 0.666571 | 3.1:-59197414 | None:intergenic |
| TCCAACGATAGGAACCTCAA+AGG | 0.670850 | 3.1:-59197457 | None:intergenic |
| ATTGTCGCTTTCCAGTACCA+TGG | 0.681766 | 3.1:+59197336 | MS.gene039963:CDS |
| TTCGAAATCTACTTCCACCA+TGG | 0.688810 | 3.1:-59197353 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGATATATGCTATAATAA+AGG | + | chr3.1:59197534-59197553 | MS.gene039963:intron | 15.0% |
| !!! | ACAGTTTTTTTCTTTTTTTT+AGG | + | chr3.1:59197574-59197593 | MS.gene039963:intron | 15.0% |
| !!! | CAGTTTTTTTCTTTTTTTTA+GGG | + | chr3.1:59197575-59197594 | MS.gene039963:intron | 15.0% |
| !! | AAAAAACAATAAACAACCTT+AGG | - | chr3.1:59197501-59197520 | None:intergenic | 20.0% |
| ! | AAGAATTTGCCAAAAGTATG+AGG | - | chr3.1:59197605-59197624 | None:intergenic | 30.0% |
| ! | ACTTTTGGCAAATTCTTTAG+AGG | + | chr3.1:59197608-59197627 | MS.gene039963:CDS | 30.0% |
| ! | CTTTTGGCAAATTCTTTAGA+GGG | + | chr3.1:59197609-59197628 | MS.gene039963:CDS | 30.0% |
| !! | ATCGTTTTATTGTACCTTTG+AGG | + | chr3.1:59197443-59197462 | MS.gene039963:CDS | 30.0% |
| TCTGTATATTTGAGTCTCAC+AGG | - | chr3.1:59197683-59197702 | None:intergenic | 35.0% | |
| ACCCTATCATTCAATGAGTG+CGG | + | chr3.1:59197416-59197435 | MS.gene039963:CDS | 40.0% | |
| ATGTGATGTTCGGAAAAGGT+TGG | + | chr3.1:59197717-59197736 | MS.gene039963:CDS | 40.0% | |
| CACGATTACGTAATGTTGTG+CGG | + | chr3.1:59197638-59197657 | MS.gene039963:CDS | 40.0% | |
| CCAAATGTGATGTTCGGAAA+AGG | + | chr3.1:59197713-59197732 | MS.gene039963:CDS | 40.0% | |
| CCCATACCAAATGTGATGTT+CGG | + | chr3.1:59197707-59197726 | MS.gene039963:CDS | 40.0% | |
| CCGAACATCACATTTGGTAT+GGG | - | chr3.1:59197710-59197729 | None:intergenic | 40.0% | |
| CGAACATCACATTTGGTATG+GGG | - | chr3.1:59197709-59197728 | None:intergenic | 40.0% | |
| CGATTGTTCTACGAGTTTAG+TGG | + | chr3.1:59197306-59197325 | MS.gene039963:CDS | 40.0% | |
| CTTGAAAAACAACACGTCTC+CGG | - | chr3.1:59197774-59197793 | None:intergenic | 40.0% | |
| TCCGAACATCACATTTGGTA+TGG | - | chr3.1:59197711-59197730 | None:intergenic | 40.0% | |
| TTCGAAATCTACTTCCACCA+TGG | - | chr3.1:59197356-59197375 | None:intergenic | 40.0% | |
| TTGTGTTGCCAATGGTATTG+AGG | + | chr3.1:59197748-59197767 | MS.gene039963:CDS | 40.0% | |
| ! | ACGAGTTTTTGTGTTGCCAA+TGG | + | chr3.1:59197740-59197759 | MS.gene039963:CDS | 40.0% |
| ! | CCTTTTCCGAACATCACATT+TGG | - | chr3.1:59197716-59197735 | None:intergenic | 40.0% |
| ! | TAGGGACTACCTCATACTTT+TGG | + | chr3.1:59197593-59197612 | MS.gene039963:intron | 40.0% |
| ! | TTTCAAGTGCAAGTGTGTCA+TGG | + | chr3.1:59197787-59197806 | MS.gene039963:CDS | 40.0% |
| ATCTACTTCCACCATGGTAC+TGG | - | chr3.1:59197350-59197369 | None:intergenic | 45.0% | |
| CTCATAACGTCGTTGACAGA+TGG | + | chr3.1:59197270-59197289 | MS.gene039963:CDS | 45.0% | |
| GCACTCATTGAATGATAGGG+TGG | - | chr3.1:59197417-59197436 | None:intergenic | 45.0% | |
| GCCGCACTCATTGAATGATA+GGG | - | chr3.1:59197420-59197439 | None:intergenic | 45.0% | |
| GTCGATCGATATCCAACGAT+AGG | - | chr3.1:59197471-59197490 | None:intergenic | 45.0% | |
| TAACGTCGTTGACAGATGGT+TGG | + | chr3.1:59197274-59197293 | MS.gene039963:CDS | 45.0% | |
| TCCAACGATAGGAACCTCAA+AGG | - | chr3.1:59197460-59197479 | None:intergenic | 45.0% | |
| TCGATCGACGAAATCACCTA+AGG | + | chr3.1:59197482-59197501 | MS.gene039963:CDS | 45.0% | |
| TGCCGCACTCATTGAATGAT+AGG | - | chr3.1:59197421-59197440 | None:intergenic | 45.0% | |
| !! | ACCTTTGAGGTTCCTATCGT+TGG | + | chr3.1:59197456-59197475 | MS.gene039963:CDS | 45.0% |
| !! | ATTGTCGCTTTCCAGTACCA+TGG | + | chr3.1:59197336-59197355 | MS.gene039963:CDS | 45.0% |
| ! | GTTGCCAATGGTATTGAGGC+CGG | + | chr3.1:59197752-59197771 | MS.gene039963:CDS | 50.0% |
| GTCTCCGGCCTCAATACCAT+TGG | - | chr3.1:59197759-59197778 | None:intergenic | 55.0% | |
| ! | GTCGCTTTCCAGTACCATGG+TGG | + | chr3.1:59197339-59197358 | MS.gene039963:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 59197255 | 59197841 | 59197255 | ID=MS.gene039963 |
| chr3.1 | mRNA | 59197255 | 59197841 | 59197255 | ID=MS.gene039963.t1;Parent=MS.gene039963 |
| chr3.1 | exon | 59197255 | 59197503 | 59197255 | ID=MS.gene039963.t1.exon1;Parent=MS.gene039963.t1 |
| chr3.1 | CDS | 59197255 | 59197503 | 59197255 | ID=cds.MS.gene039963.t1;Parent=MS.gene039963.t1 |
| chr3.1 | exon | 59197596 | 59197841 | 59197596 | ID=MS.gene039963.t1.exon2;Parent=MS.gene039963.t1 |
| chr3.1 | CDS | 59197596 | 59197841 | 59197596 | ID=cds.MS.gene039963.t1;Parent=MS.gene039963.t1 |
| Gene Sequence |
| Protein sequence |
