Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040274.t1 | GAU40839.1 | 58 | 176 | 72 | 1 | 7 | 182 | 29 | 202 | 4.60E-49 | 204.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040274.t1 | Q6NQJ7 | 36.8 | 106 | 64 | 2 | 48 | 151 | 3 | 107 | 2.9e-12 | 73.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040274.t1 | A0A2Z6P9Q3 | 58.0 | 176 | 72 | 1 | 7 | 182 | 29 | 202 | 3.3e-49 | 204.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene040274.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040274.t1 | MTR_7g031620 | 74.390 | 82 | 21 | 0 | 47 | 128 | 1 | 82 | 1.32e-35 | 122 |
| MS.gene040274.t1 | MTR_7g446060 | 60.938 | 64 | 25 | 0 | 119 | 182 | 2 | 65 | 5.99e-20 | 83.6 |
| MS.gene040274.t1 | MTR_0856s0010 | 31.496 | 127 | 86 | 1 | 42 | 167 | 34 | 160 | 1.51e-13 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040274.t1 | AT1G76320 | 36.792 | 106 | 64 | 2 | 48 | 151 | 3 | 107 | 7.31e-15 | 74.7 |
| MS.gene040274.t1 | AT1G76320 | 36.792 | 106 | 64 | 2 | 48 | 151 | 3 | 107 | 7.60e-15 | 74.3 |
| MS.gene040274.t1 | AT1G76320 | 36.792 | 106 | 64 | 2 | 48 | 151 | 3 | 107 | 7.60e-15 | 74.3 |
| MS.gene040274.t1 | AT1G76320 | 36.792 | 106 | 64 | 2 | 48 | 151 | 3 | 107 | 1.17e-14 | 73.9 |
Find 28 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTCATAAGCATTGAATT+TGG | 0.196629 | 7.1:-65822570 | None:intergenic |
| AATGCATGTATTTCTTTAGA+TGG | 0.249459 | 7.1:+65822167 | MS.gene040274:CDS |
| CTAAAATAGCGTGCTTTGTC+AGG | 0.272247 | 7.1:-65822239 | None:intergenic |
| TATGCCCAATCGATGGGTTT+TGG | 0.287180 | 7.1:+65821999 | MS.gene040274:CDS |
| TACCTGTAACAATTCAAAAT+AGG | 0.377069 | 7.1:-65822722 | None:intergenic |
| TGCATGACTTGAGAATAAAA+TGG | 0.407764 | 7.1:-65822688 | None:intergenic |
| GCTTATGAGTGAAGTAAATC+AGG | 0.434328 | 7.1:+65822581 | MS.gene040274:CDS |
| AAGCATTGAATTTGGATTGA+AGG | 0.439628 | 7.1:-65822562 | None:intergenic |
| AGCATTGAATTTGGATTGAA+GGG | 0.452863 | 7.1:-65822561 | None:intergenic |
| CATTCAATCTAGCCTTGCAT+TGG | 0.455682 | 7.1:-65822148 | None:intergenic |
| GTTCAAGTAGTTGATGTAGT+TGG | 0.457215 | 7.1:+65822365 | MS.gene040274:CDS |
| AACTCCAAAACCCATCGATT+GGG | 0.481007 | 7.1:-65822003 | None:intergenic |
| ATTCAATCTAGCCTTGCATT+GGG | 0.485892 | 7.1:-65822147 | None:intergenic |
| TAACTCCAAAACCCATCGAT+TGG | 0.508337 | 7.1:-65822004 | None:intergenic |
| CAAGAGTTATGCCCAATCGA+TGG | 0.518094 | 7.1:+65821992 | MS.gene040274:CDS |
| ATTTGTATCTTCTGATGAAG+AGG | 0.520293 | 7.1:+65821851 | MS.gene040274:CDS |
| TATTCTCAAGTCATGCATCA+TGG | 0.520944 | 7.1:+65822694 | MS.gene040274:CDS |
| AAGAGTTATGCCCAATCGAT+GGG | 0.531992 | 7.1:+65821993 | MS.gene040274:CDS |
| TGGTTCCTCGTTTGAAAAGG+AGG | 0.532707 | 7.1:+65822276 | MS.gene040274:CDS |
| TAAAATAGCGTGCTTTGTCA+GGG | 0.562727 | 7.1:-65822238 | None:intergenic |
| TTAGAACTCAATGATCAAGC+TGG | 0.567016 | 7.1:+65822299 | MS.gene040274:CDS |
| TTTACTCTAGCATGTAGTTG+TGG | 0.583557 | 7.1:+65822068 | MS.gene040274:CDS |
| CGTGTTATTGATGACGACAA+CGG | 0.597290 | 7.1:-65822853 | None:intergenic |
| GATGAAGAGGAAATCAGAGA+GGG | 0.625109 | 7.1:+65821864 | MS.gene040274:CDS |
| CTAAATATAGTTCCAACCTG+AGG | 0.648571 | 7.1:-65821942 | None:intergenic |
| TTTGGGAGGAATGGACATGG+TGG | 0.651520 | 7.1:+65822522 | MS.gene040274:CDS |
| TGATGAAGAGGAAATCAGAG+AGG | 0.679814 | 7.1:+65821863 | MS.gene040274:CDS |
| ACGACAACGGATCATTCACA+TGG | 0.697567 | 7.1:-65822840 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATTGATTTATCATATAA+AGG | - | chr7.1:65821687-65821706 | None:intergenic | 10.0% |
| !!! | TTAATACATATATGTTTTTA+GGG | + | chr7.1:65822486-65822505 | MS.gene040274:intron | 10.0% |
| !!! | TTTAATACATATATGTTTTT+AGG | + | chr7.1:65822485-65822504 | MS.gene040274:intron | 10.0% |
| !!! | TAATACATATATGTTTTTAG+GGG | + | chr7.1:65822487-65822506 | MS.gene040274:intron | 15.0% |
| !!! | TTTTTAATTTATTGCATTAG+TGG | + | chr7.1:65821821-65821840 | MS.gene040274:intron | 15.0% |
| !! | TAAGCATTAAATACAAATCT+AGG | - | chr7.1:65821741-65821760 | None:intergenic | 20.0% |
| !! | TTCATGTAACATTAATATTG+TGG | + | chr7.1:65822633-65822652 | MS.gene040274:intron | 20.0% |
| !!! | AATACATATATGTTTTTAGG+GGG | + | chr7.1:65822488-65822507 | MS.gene040274:intron | 20.0% |
| !!! | TTTTAGAAGTAGTAAGTTTT+TGG | + | chr7.1:65822256-65822275 | MS.gene040274:CDS | 20.0% |
| !!! | TTTTGATATTGAAACTGATT+TGG | + | chr7.1:65821710-65821729 | MS.gene040274:intron | 20.0% |
| ! | AAAATGGAACCTAAATAACA+TGG | - | chr7.1:65822675-65822694 | None:intergenic | 25.0% |
| ! | AATGCATGTATTTCTTTAGA+TGG | + | chr7.1:65822167-65822186 | MS.gene040274:CDS | 25.0% |
| ! | TAAGTTAACTACCTTAAACT+AGG | + | chr7.1:65821628-65821647 | MS.gene040274:intron | 25.0% |
| ! | TACCTGTAACAATTCAAAAT+AGG | - | chr7.1:65822725-65822744 | None:intergenic | 25.0% |
| !!! | AAGCATTTTCCATGTTATTT+AGG | + | chr7.1:65822663-65822682 | MS.gene040274:intron | 25.0% |
| !!! | TGTTAGTTTGTTCTTTTTGT+AGG | + | chr7.1:65822810-65822829 | MS.gene040274:intron | 25.0% |
| !!! | TTCCTATTTTGAATTGTTAC+AGG | + | chr7.1:65822720-65822739 | MS.gene040274:CDS | 25.0% |
| AGTAACAAAAATGCAGATGA+TGG | + | chr7.1:65822035-65822054 | MS.gene040274:CDS | 30.0% | |
| ATTTGTATCTTCTGATGAAG+AGG | + | chr7.1:65821851-65821870 | MS.gene040274:CDS | 30.0% | |
| GTAACAAAAATGCAGATGAT+GGG | + | chr7.1:65822036-65822055 | MS.gene040274:CDS | 30.0% | |
| TGCATGACTTGAGAATAAAA+TGG | - | chr7.1:65822691-65822710 | None:intergenic | 30.0% | |
| TTTAACAAAAACCCAATGCA+AGG | + | chr7.1:65822136-65822155 | MS.gene040274:CDS | 30.0% | |
| ! | AGCATTGAATTTGGATTGAA+GGG | - | chr7.1:65822564-65822583 | None:intergenic | 30.0% |
| ! | GGGTTTTTGTTAAAGGATTT+GGG | - | chr7.1:65822130-65822149 | None:intergenic | 30.0% |
| ! | TGGGTTTTTGTTAAAGGATT+TGG | - | chr7.1:65822131-65822150 | None:intergenic | 30.0% |
| ! | TTGCATTGGGTTTTTGTTAA+AGG | - | chr7.1:65822137-65822156 | None:intergenic | 30.0% |
| !! | AAGCATTGAATTTGGATTGA+AGG | - | chr7.1:65822565-65822584 | None:intergenic | 30.0% |
| !! | TAGGGGGAAAAAATTAGTTT+TGG | + | chr7.1:65822504-65822523 | MS.gene040274:intron | 30.0% |
| !! | TCACTCATAAGCATTGAATT+TGG | - | chr7.1:65822573-65822592 | None:intergenic | 30.0% |
| !!! | AAAATTAGTTTTGGGAGGAA+TGG | + | chr7.1:65822513-65822532 | MS.gene040274:CDS | 30.0% |
| !!! | AGGGGGAAAAAATTAGTTTT+GGG | + | chr7.1:65822505-65822524 | MS.gene040274:intron | 30.0% |
| ATGGGTGATAACCTAGTTTA+AGG | - | chr7.1:65821642-65821661 | None:intergenic | 35.0% | |
| CTAAATATAGTTCCAACCTG+AGG | - | chr7.1:65821945-65821964 | None:intergenic | 35.0% | |
| GCTTATGAGTGAAGTAAATC+AGG | + | chr7.1:65822581-65822600 | MS.gene040274:CDS | 35.0% | |
| TAAAATAGCGTGCTTTGTCA+GGG | - | chr7.1:65822241-65822260 | None:intergenic | 35.0% | |
| TATTCTCAAGTCATGCATCA+TGG | + | chr7.1:65822694-65822713 | MS.gene040274:CDS | 35.0% | |
| TCATGAAGATGAAAAACCTC+AGG | + | chr7.1:65821926-65821945 | MS.gene040274:CDS | 35.0% | |
| TTAGAACTCAATGATCAAGC+TGG | + | chr7.1:65822299-65822318 | MS.gene040274:CDS | 35.0% | |
| TTTACTCTAGCATGTAGTTG+TGG | + | chr7.1:65822068-65822087 | MS.gene040274:CDS | 35.0% | |
| ! | GTTCAAGTAGTTGATGTAGT+TGG | + | chr7.1:65822365-65822384 | MS.gene040274:CDS | 35.0% |
| !! | ATTCAATCTAGCCTTGCATT+GGG | - | chr7.1:65822150-65822169 | None:intergenic | 35.0% |
| !! | GTAGTTTGATCAGTTGATGA+GGG | - | chr7.1:65821665-65821684 | None:intergenic | 35.0% |
| !!! | GGGAAAAAATTAGTTTTGGG+AGG | + | chr7.1:65822508-65822527 | MS.gene040274:CDS | 35.0% |
| !!! | TTTTGGTTCCTCGTTTGAAA+AGG | + | chr7.1:65822273-65822292 | MS.gene040274:CDS | 35.0% |
| AAGAGTTATGCCCAATCGAT+GGG | + | chr7.1:65821993-65822012 | MS.gene040274:CDS | 40.0% | |
| CTAAAATAGCGTGCTTTGTC+AGG | - | chr7.1:65822242-65822261 | None:intergenic | 40.0% | |
| GAAGATGAAAAACCTCAGGT+TGG | + | chr7.1:65821930-65821949 | MS.gene040274:CDS | 40.0% | |
| GATGAAGAGGAAATCAGAGA+GGG | + | chr7.1:65821864-65821883 | MS.gene040274:CDS | 40.0% | |
| TAACTCCAAAACCCATCGAT+TGG | - | chr7.1:65822007-65822026 | None:intergenic | 40.0% | |
| TGATGAAGAGGAAATCAGAG+AGG | + | chr7.1:65821863-65821882 | MS.gene040274:CDS | 40.0% | |
| ! | AACTCCAAAACCCATCGATT+GGG | - | chr7.1:65822006-65822025 | None:intergenic | 40.0% |
| ! | GGTAGTTTGATCAGTTGATG+AGG | - | chr7.1:65821666-65821685 | None:intergenic | 40.0% |
| ! | TCTAACCTCCTTTTCAAACG+AGG | - | chr7.1:65822284-65822303 | None:intergenic | 40.0% |
| !! | CATTCAATCTAGCCTTGCAT+TGG | - | chr7.1:65822151-65822170 | None:intergenic | 40.0% |
| !! | CGTGTTATTGATGACGACAA+CGG | - | chr7.1:65822856-65822875 | None:intergenic | 40.0% |
| !! | TTGATCAGTTGATGAGGGAT+GGG | - | chr7.1:65821660-65821679 | None:intergenic | 40.0% |
| !! | TTTGATCAGTTGATGAGGGA+TGG | - | chr7.1:65821661-65821680 | None:intergenic | 40.0% |
| ACGACAACGGATCATTCACA+TGG | - | chr7.1:65822843-65822862 | None:intergenic | 45.0% | |
| CAAGAGTTATGCCCAATCGA+TGG | + | chr7.1:65821992-65822011 | MS.gene040274:CDS | 45.0% | |
| TGGTTCCTCGTTTGAAAAGG+AGG | + | chr7.1:65822276-65822295 | MS.gene040274:CDS | 45.0% | |
| !! | TATGCCCAATCGATGGGTTT+TGG | + | chr7.1:65821999-65822018 | MS.gene040274:CDS | 45.0% |
| !!! | AGTTTTGGGAGGAATGGACA+TGG | + | chr7.1:65822519-65822538 | MS.gene040274:CDS | 45.0% |
| TTTGGGAGGAATGGACATGG+TGG | + | chr7.1:65822522-65822541 | MS.gene040274:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 65821605 | 65822885 | 65821605 | ID=MS.gene040274 |
| chr7.1 | mRNA | 65821605 | 65822885 | 65821605 | ID=MS.gene040274.t1;Parent=MS.gene040274 |
| chr7.1 | exon | 65821605 | 65821626 | 65821605 | ID=MS.gene040274.t1.exon1;Parent=MS.gene040274.t1 |
| chr7.1 | CDS | 65821605 | 65821626 | 65821605 | ID=cds.MS.gene040274.t1;Parent=MS.gene040274.t1 |
| chr7.1 | exon | 65821841 | 65822406 | 65821841 | ID=MS.gene040274.t1.exon2;Parent=MS.gene040274.t1 |
| chr7.1 | CDS | 65821841 | 65822406 | 65821841 | ID=cds.MS.gene040274.t1;Parent=MS.gene040274.t1 |
| chr7.1 | exon | 65822507 | 65822602 | 65822507 | ID=MS.gene040274.t1.exon3;Parent=MS.gene040274.t1 |
| chr7.1 | CDS | 65822507 | 65822602 | 65822507 | ID=cds.MS.gene040274.t1;Parent=MS.gene040274.t1 |
| chr7.1 | exon | 65822685 | 65822741 | 65822685 | ID=MS.gene040274.t1.exon4;Parent=MS.gene040274.t1 |
| chr7.1 | CDS | 65822685 | 65822741 | 65822685 | ID=cds.MS.gene040274.t1;Parent=MS.gene040274.t1 |
| chr7.1 | exon | 65822832 | 65822885 | 65822832 | ID=MS.gene040274.t1.exon5;Parent=MS.gene040274.t1 |
| chr7.1 | CDS | 65822832 | 65822885 | 65822832 | ID=cds.MS.gene040274.t1;Parent=MS.gene040274.t1 |
| Gene Sequence |
| Protein sequence |
