Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040366.t1 | XP_024634520.1 | 80.1 | 744 | 146 | 2 | 3 | 745 | 12 | 754 | 0.00E+00 | 1212.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040366.t1 | Q9SZL8 | 39.9 | 586 | 329 | 9 | 18 | 587 | 59 | 637 | 2.2e-126 | 454.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040366.t1 | A0A2Z6P1T9 | 79.3 | 731 | 151 | 0 | 14 | 744 | 5 | 735 | 0.0e+00 | 1182.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene040366.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040366.t1 | MTR_6g048540 | 85.470 | 117 | 17 | 0 | 559 | 675 | 106 | 222 | 6.84e-63 | 210 |
| MS.gene040366.t1 | MTR_6g048540 | 95.876 | 97 | 4 | 0 | 1 | 97 | 1 | 97 | 3.45e-58 | 197 |
| MS.gene040366.t1 | MTR_0734s0010 | 45.078 | 193 | 101 | 2 | 551 | 743 | 1 | 188 | 7.11e-47 | 165 |
| MS.gene040366.t1 | MTR_0853s0010 | 52.083 | 144 | 68 | 1 | 19 | 162 | 16 | 158 | 3.15e-44 | 157 |
| MS.gene040366.t1 | MTR_1g019850 | 51.852 | 162 | 64 | 2 | 3 | 164 | 51 | 198 | 1.04e-43 | 158 |
| MS.gene040366.t1 | MTR_6g051425 | 31.541 | 279 | 159 | 7 | 42 | 296 | 28 | 298 | 1.43e-37 | 144 |
| MS.gene040366.t1 | MTR_3g020080 | 48.667 | 150 | 54 | 4 | 376 | 519 | 3 | 135 | 1.95e-29 | 114 |
| MS.gene040366.t1 | MTR_4g035820 | 25.660 | 265 | 174 | 5 | 21 | 270 | 68 | 324 | 2.75e-21 | 99.4 |
| MS.gene040366.t1 | MTR_4g035820 | 39.604 | 101 | 55 | 2 | 404 | 501 | 327 | 424 | 8.74e-12 | 68.9 |
| MS.gene040366.t1 | MTR_0558s0020 | 24.242 | 264 | 178 | 4 | 25 | 266 | 33 | 296 | 1.03e-20 | 94.0 |
| MS.gene040366.t1 | MTR_1g070355 | 27.615 | 239 | 154 | 7 | 33 | 260 | 79 | 309 | 8.15e-20 | 94.4 |
| MS.gene040366.t1 | MTR_3g450850 | 26.437 | 261 | 164 | 9 | 31 | 272 | 50 | 301 | 3.88e-17 | 84.0 |
| MS.gene040366.t1 | MTR_8g446020 | 25.581 | 172 | 123 | 1 | 100 | 266 | 85 | 256 | 8.89e-17 | 81.3 |
| MS.gene040366.t1 | MTR_6g022070 | 31.395 | 172 | 112 | 2 | 320 | 490 | 1 | 167 | 3.26e-16 | 81.3 |
| MS.gene040366.t1 | MTR_5g076070 | 25.506 | 247 | 164 | 6 | 19 | 254 | 45 | 282 | 2.77e-15 | 77.4 |
| MS.gene040366.t1 | MTR_8g466860 | 84.615 | 39 | 6 | 0 | 390 | 428 | 80 | 118 | 3.38e-15 | 73.2 |
| MS.gene040366.t1 | MTR_7g446510 | 28.571 | 175 | 120 | 2 | 105 | 274 | 155 | 329 | 4.30e-15 | 77.8 |
| MS.gene040366.t1 | MTR_7g446480 | 29.545 | 176 | 117 | 4 | 105 | 274 | 140 | 314 | 1.75e-14 | 75.5 |
| MS.gene040366.t1 | MTR_6g090505 | 22.831 | 219 | 158 | 3 | 58 | 265 | 12 | 230 | 3.92e-13 | 72.0 |
| MS.gene040366.t1 | MTR_8g465780 | 30.380 | 158 | 97 | 4 | 397 | 551 | 1 | 148 | 4.02e-13 | 68.9 |
| MS.gene040366.t1 | MTR_2g024230 | 45.570 | 79 | 38 | 1 | 3 | 81 | 70 | 143 | 5.90e-12 | 65.1 |
| MS.gene040366.t1 | MTR_1g013060 | 26.818 | 220 | 144 | 7 | 18 | 226 | 16 | 229 | 1.09e-11 | 65.9 |
| MS.gene040366.t1 | MTR_2g098880 | 29.197 | 137 | 76 | 4 | 27 | 156 | 72 | 194 | 4.35e-11 | 64.7 |
| MS.gene040366.t1 | MTR_2g098880 | 29.197 | 137 | 76 | 4 | 27 | 156 | 60 | 182 | 4.67e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040366.t1 | AT4G38180 | 39.932 | 586 | 329 | 9 | 18 | 587 | 59 | 637 | 2.26e-153 | 466 |
| MS.gene040366.t1 | AT2G27110 | 32.268 | 657 | 392 | 8 | 5 | 637 | 2 | 629 | 2.37e-120 | 381 |
| MS.gene040366.t1 | AT2G27110 | 32.268 | 657 | 392 | 8 | 5 | 637 | 2 | 629 | 2.37e-120 | 381 |
| MS.gene040366.t1 | AT4G15090 | 34.899 | 596 | 364 | 7 | 5 | 587 | 28 | 612 | 3.62e-113 | 362 |
| MS.gene040366.t1 | AT4G15090 | 34.899 | 596 | 364 | 7 | 5 | 587 | 28 | 612 | 3.62e-113 | 362 |
| MS.gene040366.t1 | AT4G15090 | 34.899 | 596 | 364 | 7 | 5 | 587 | 28 | 612 | 3.62e-113 | 362 |
| MS.gene040366.t1 | AT4G15090 | 34.899 | 596 | 364 | 7 | 5 | 587 | 28 | 612 | 3.62e-113 | 362 |
| MS.gene040366.t1 | AT4G15090 | 34.899 | 596 | 364 | 7 | 5 | 587 | 28 | 612 | 3.62e-113 | 362 |
| MS.gene040366.t1 | AT3G22170 | 34.673 | 597 | 350 | 12 | 6 | 583 | 42 | 617 | 4.69e-113 | 362 |
| MS.gene040366.t1 | AT3G22170 | 34.673 | 597 | 350 | 12 | 6 | 583 | 42 | 617 | 4.69e-113 | 362 |
| MS.gene040366.t1 | AT2G27110 | 38.952 | 439 | 263 | 2 | 199 | 637 | 51 | 484 | 1.17e-109 | 349 |
| MS.gene040366.t1 | AT1G76320 | 31.852 | 675 | 432 | 11 | 32 | 696 | 1 | 657 | 4.98e-107 | 341 |
| MS.gene040366.t1 | AT1G76320 | 31.852 | 675 | 432 | 11 | 32 | 696 | 1 | 657 | 1.80e-106 | 342 |
| MS.gene040366.t1 | AT1G76320 | 31.852 | 675 | 432 | 11 | 32 | 696 | 1 | 657 | 1.80e-106 | 342 |
| MS.gene040366.t1 | AT1G76320 | 31.852 | 675 | 432 | 11 | 32 | 696 | 1 | 657 | 2.05e-106 | 341 |
| MS.gene040366.t1 | AT4G38170 | 36.809 | 470 | 286 | 4 | 203 | 667 | 21 | 484 | 1.04e-99 | 318 |
| MS.gene040366.t1 | AT3G06250 | 32.419 | 620 | 358 | 12 | 26 | 637 | 188 | 754 | 6.84e-98 | 320 |
| MS.gene040366.t1 | AT3G06250 | 32.419 | 620 | 358 | 12 | 26 | 637 | 188 | 754 | 6.84e-98 | 320 |
| MS.gene040366.t1 | AT3G06250 | 32.419 | 620 | 358 | 12 | 26 | 637 | 188 | 754 | 6.84e-98 | 320 |
| MS.gene040366.t1 | AT5G18960 | 31.994 | 622 | 363 | 13 | 25 | 637 | 208 | 778 | 2.27e-97 | 319 |
| MS.gene040366.t1 | AT5G18960 | 31.994 | 622 | 363 | 13 | 25 | 637 | 208 | 778 | 2.27e-97 | 319 |
| MS.gene040366.t1 | AT2G32250 | 29.985 | 667 | 397 | 10 | 1 | 650 | 1 | 614 | 4.91e-94 | 310 |
| MS.gene040366.t1 | AT2G32250 | 29.985 | 667 | 397 | 10 | 1 | 650 | 1 | 614 | 8.69e-94 | 310 |
| MS.gene040366.t1 | AT2G32250 | 29.985 | 667 | 397 | 10 | 1 | 650 | 1 | 614 | 8.69e-94 | 310 |
| MS.gene040366.t1 | AT2G32250 | 29.985 | 667 | 397 | 10 | 1 | 650 | 1 | 614 | 8.96e-94 | 310 |
| MS.gene040366.t1 | AT2G32250 | 29.985 | 667 | 397 | 10 | 1 | 650 | 1 | 614 | 8.96e-94 | 310 |
| MS.gene040366.t1 | AT2G32250 | 29.985 | 667 | 397 | 10 | 1 | 650 | 1 | 614 | 8.96e-94 | 310 |
| MS.gene040366.t1 | AT2G32250 | 29.712 | 626 | 380 | 7 | 32 | 650 | 1 | 573 | 7.87e-92 | 303 |
| MS.gene040366.t1 | AT2G32250 | 29.712 | 626 | 380 | 7 | 32 | 650 | 1 | 573 | 9.49e-92 | 303 |
| MS.gene040366.t1 | AT4G19990 | 32.870 | 575 | 336 | 9 | 25 | 583 | 18 | 558 | 3.00e-91 | 302 |
| MS.gene040366.t1 | AT4G19990 | 32.870 | 575 | 336 | 9 | 25 | 583 | 18 | 558 | 3.00e-91 | 302 |
| MS.gene040366.t1 | AT4G19990 | 32.870 | 575 | 336 | 9 | 25 | 583 | 18 | 558 | 4.27e-91 | 301 |
| MS.gene040366.t1 | AT4G19990 | 32.870 | 575 | 336 | 9 | 25 | 583 | 32 | 572 | 5.64e-91 | 301 |
| MS.gene040366.t1 | AT4G19990 | 32.870 | 575 | 336 | 9 | 25 | 583 | 18 | 558 | 6.74e-91 | 301 |
| MS.gene040366.t1 | AT4G19990 | 32.870 | 575 | 336 | 9 | 25 | 583 | 18 | 558 | 6.74e-91 | 301 |
| MS.gene040366.t1 | AT1G52520 | 30.836 | 574 | 356 | 14 | 28 | 582 | 85 | 636 | 5.62e-88 | 292 |
| MS.gene040366.t1 | AT4G19990 | 34.356 | 489 | 287 | 5 | 97 | 583 | 45 | 501 | 2.74e-86 | 287 |
| MS.gene040366.t1 | AT1G80010 | 30.017 | 593 | 363 | 13 | 24 | 585 | 91 | 662 | 2.49e-85 | 285 |
| MS.gene040366.t1 | AT1G10240 | 28.680 | 659 | 401 | 15 | 25 | 647 | 45 | 670 | 2.87e-85 | 284 |
| MS.gene040366.t1 | AT1G10240 | 28.680 | 659 | 401 | 15 | 25 | 647 | 45 | 670 | 2.87e-85 | 284 |
| MS.gene040366.t1 | AT5G28530 | 27.288 | 579 | 385 | 8 | 28 | 583 | 55 | 620 | 1.08e-65 | 231 |
| MS.gene040366.t1 | AT5G28530 | 27.116 | 579 | 382 | 8 | 28 | 583 | 55 | 616 | 3.73e-65 | 229 |
| MS.gene040366.t1 | AT5G28530 | 27.116 | 579 | 382 | 8 | 28 | 583 | 55 | 616 | 3.73e-65 | 229 |
| MS.gene040366.t1 | AT5G28530 | 25.088 | 570 | 346 | 10 | 28 | 583 | 55 | 557 | 4.42e-46 | 174 |
| MS.gene040366.t1 | AT5G28530 | 24.912 | 570 | 343 | 10 | 28 | 583 | 55 | 553 | 1.00e-45 | 173 |
| MS.gene040366.t1 | AT3G59470 | 31.852 | 135 | 75 | 5 | 5 | 132 | 47 | 171 | 2.03e-11 | 65.5 |
| MS.gene040366.t1 | AT3G59470 | 31.852 | 135 | 75 | 5 | 5 | 132 | 47 | 171 | 2.03e-11 | 65.5 |
| MS.gene040366.t1 | AT3G59470 | 31.852 | 135 | 75 | 5 | 5 | 132 | 59 | 183 | 2.56e-11 | 65.5 |
Find 106 sgRNAs with CRISPR-Local
Find 136 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCCTTGTTGAACAGTTTA+AGG | 0.134754 | 7.4:-71615367 | MS.gene040366:CDS |
| ATGTCAACTGTTTGAATATT+TGG | 0.186130 | 7.4:-71614188 | MS.gene040366:CDS |
| TTTAAGTTCTCGCTTGAAAA+TGG | 0.195510 | 7.4:+71614753 | None:intergenic |
| CCCATCCTTCTTTCTTCCTT+TGG | 0.210149 | 7.4:+71613748 | None:intergenic |
| GGGATTCAGCTTTCTCTAAT+AGG | 0.233911 | 7.4:-71615299 | MS.gene040366:CDS |
| GAACTTAAAAGTTGCATTCA+TGG | 0.260689 | 7.4:-71614739 | MS.gene040366:CDS |
| TTGATCAGTTATGACAGATT+TGG | 0.270381 | 7.4:+71614894 | None:intergenic |
| AGATTAGCATACCAACAATT+TGG | 0.281390 | 7.4:-71615102 | MS.gene040366:CDS |
| CAGAGGCGATGTCTAGTTTC+TGG | 0.281760 | 7.4:+71614835 | None:intergenic |
| TGTCAACTGTTTGAATATTT+GGG | 0.293427 | 7.4:-71614187 | MS.gene040366:CDS |
| GCCTCTGCTTGTGGCATATT+CGG | 0.312161 | 7.4:-71614819 | MS.gene040366:CDS |
| AGATGAATGGAAGCGTTTAA+TGG | 0.328762 | 7.4:-71614692 | MS.gene040366:CDS |
| TGATCAGTTATGACAGATTT+GGG | 0.347589 | 7.4:+71614895 | None:intergenic |
| TAGCCTGATTTGTGTCATCT+TGG | 0.357525 | 7.4:+71615565 | None:intergenic |
| GATATTGTGTAGACATATAT+TGG | 0.363885 | 7.4:-71614164 | MS.gene040366:CDS |
| GGATTCAGCTTTCTCTAATA+GGG | 0.365034 | 7.4:-71615298 | MS.gene040366:CDS |
| GGGGTACAACGGAAGGTAAT+TGG | 0.367395 | 7.4:-71615488 | MS.gene040366:CDS |
| AAGAAGGATGCATGTTCAAC+AGG | 0.368503 | 7.4:-71614002 | MS.gene040366:CDS |
| TTTAATGGTTGAGTATAATC+TGG | 0.372730 | 7.4:-71614677 | MS.gene040366:CDS |
| TGGTGATGGAATTTCTCCTT+TGG | 0.376539 | 7.4:-71613861 | MS.gene040366:CDS |
| TTCTTCCTTTGGTTTGTGCT+TGG | 0.378150 | 7.4:+71613759 | None:intergenic |
| CTTTCTCTAATAGGGATTGT+TGG | 0.393553 | 7.4:-71615290 | MS.gene040366:CDS |
| AATAAATTTGATTGACATCC+TGG | 0.396334 | 7.4:+71613831 | None:intergenic |
| ATGCCCTTTGCCCCGTTCGT+TGG | 0.398777 | 7.4:-71615033 | MS.gene040366:CDS |
| GTTGAACAGTTTAAGGAAGA+AGG | 0.402857 | 7.4:-71615360 | MS.gene040366:CDS |
| GAAAACAATGAATGGCTTCA+AGG | 0.415447 | 7.4:-71614655 | MS.gene040366:CDS |
| AAAACCGAAAAGAGCTATCT+TGG | 0.417824 | 7.4:-71615640 | MS.gene040366:CDS |
| TTCGAGAATCTTCATCGCAT+TGG | 0.421036 | 7.4:+71615157 | None:intergenic |
| GAACAGCCAGAACTCACTAT+TGG | 0.421931 | 7.4:-71613791 | MS.gene040366:CDS |
| TAAATAGTGAACCTTGTAAT+GGG | 0.426162 | 7.4:-71615749 | MS.gene040366:CDS |
| ATCTCATGAAAATATTTCAA+TGG | 0.427085 | 7.4:-71615796 | MS.gene040366:CDS |
| TTCTCCTTTGGAATCTAGCC+AGG | 0.428333 | 7.4:-71613849 | MS.gene040366:CDS |
| GGGTTTCCAATAGTGAGTTC+TGG | 0.429369 | 7.4:+71613785 | None:intergenic |
| AAATGACATCTCCAAATTGT+TGG | 0.437011 | 7.4:+71615091 | None:intergenic |
| AATTCACTTCTATTATAAAC+CGG | 0.443437 | 7.4:+71614604 | None:intergenic |
| CTTTGGTTTGTGCTTGGCGT+GGG | 0.443719 | 7.4:+71613765 | None:intergenic |
| GATTATTCAATCCAACGAAC+GGG | 0.446022 | 7.4:+71615022 | None:intergenic |
| TCTTGCAAGTTCTGCTGTTG+TGG | 0.446841 | 7.4:+71613624 | None:intergenic |
| AAAGGGCATACTGTACTTGT+TGG | 0.452825 | 7.4:+71615047 | None:intergenic |
| AAGCTTCACTTACTGTTACA+AGG | 0.461078 | 7.4:-71615509 | MS.gene040366:CDS |
| AAGAGTTCATCTTCTCGAAC+CGG | 0.461559 | 7.4:-71615537 | MS.gene040366:CDS |
| AATCCAAGATGACACAAATC+AGG | 0.464191 | 7.4:-71615568 | MS.gene040366:CDS |
| TTTAATTCCTCGCCAACATT+CGG | 0.464350 | 7.4:+71615768 | None:intergenic |
| CACAACCAAATAAAATTGAT+TGG | 0.468390 | 7.4:+71614995 | None:intergenic |
| ATTTGTGTTAAAATTTGAAA+AGG | 0.469070 | 7.4:-71614494 | MS.gene040366:CDS |
| TCTCATGAAAATATTTCAAT+GGG | 0.469484 | 7.4:-71615795 | MS.gene040366:CDS |
| TACAATGTTTCGAAGATGAA+TGG | 0.470517 | 7.4:-71614705 | MS.gene040366:CDS |
| AGTAAGTGAAGCTTTACAAC+CGG | 0.474121 | 7.4:+71615518 | None:intergenic |
| TTGGTAATATTGCAAGCAAA+AGG | 0.478299 | 7.4:-71614145 | MS.gene040366:CDS |
| TTAAATAGTGAACCTTGTAA+TGG | 0.482835 | 7.4:-71615750 | MS.gene040366:CDS |
| TAATTATTGCAAACAGAGAC+AGG | 0.484710 | 7.4:-71615208 | MS.gene040366:CDS |
| GGAGAAATTCCATCACCAAC+AGG | 0.484890 | 7.4:+71613866 | None:intergenic |
| TTGAATTCCATCCCATTACA+AGG | 0.486817 | 7.4:+71615738 | None:intergenic |
| TCAAATTTACCTGTTGGTGA+TGG | 0.490672 | 7.4:-71613875 | MS.gene040366:CDS |
| AATCCAACGAACGGGGCAAA+GGG | 0.491248 | 7.4:+71615030 | None:intergenic |
| CGAAGTAGGAATTTAGATGT+TGG | 0.494361 | 7.4:-71615249 | MS.gene040366:CDS |
| CAATCCAACGAACGGGGCAA+AGG | 0.499412 | 7.4:+71615029 | None:intergenic |
| CCTTTGGTTTGTGCTTGGCG+TGG | 0.499884 | 7.4:+71613764 | None:intergenic |
| ATAATCTGGAAAACAATGAA+TGG | 0.501263 | 7.4:-71614663 | MS.gene040366:CDS |
| AAGAAATCAAATTTACCTGT+TGG | 0.505747 | 7.4:-71613881 | MS.gene040366:CDS |
| TTGAACAGTTTAAGGAAGAA+GGG | 0.512879 | 7.4:-71615359 | MS.gene040366:CDS |
| GGCGCTTGATAGCCGCTTAG+CGG | 0.513168 | 7.4:-71614473 | MS.gene040366:CDS |
| TGCTTTATTACAAGATGAGT+CGG | 0.514921 | 7.4:-71614974 | MS.gene040366:CDS |
| AGCTTCACTTACTGTTACAA+GGG | 0.522791 | 7.4:-71615508 | MS.gene040366:CDS |
| CCAAAGGAAGAAAGAAGGAT+GGG | 0.526798 | 7.4:-71613748 | MS.gene040366:CDS |
| ATCTTGGTATGTTCTAATGA+AGG | 0.531078 | 7.4:-71615624 | MS.gene040366:CDS |
| TCCGAATATGCCACAAGCAG+AGG | 0.532192 | 7.4:+71614818 | None:intergenic |
| AGAAAGAAGATTAAAAGAGA+AGG | 0.533498 | 7.4:-71614304 | MS.gene040366:CDS |
| TCAATGGGATCTCCGAATGT+TGG | 0.534987 | 7.4:-71615780 | MS.gene040366:CDS |
| GACACAAAGATTGCTAAATG+TGG | 0.536676 | 7.4:-71614211 | MS.gene040366:CDS |
| TAGTGAACCTTGTAATGGGA+TGG | 0.536929 | 7.4:-71615745 | MS.gene040366:CDS |
| CTAGACATCGCCTCTGCTTG+TGG | 0.538620 | 7.4:-71614828 | MS.gene040366:CDS |
| TCTTCGAAACATTGTATACT+CGG | 0.550021 | 7.4:+71614712 | None:intergenic |
| ACAAACCAAAGGAAGAAAGA+AGG | 0.551427 | 7.4:-71613753 | MS.gene040366:CDS |
| ACCAAAGGAAGAAAGAAGGA+TGG | 0.552890 | 7.4:-71613749 | MS.gene040366:CDS |
| AAAACAATGAATGGCTTCAA+GGG | 0.554540 | 7.4:-71614654 | MS.gene040366:CDS |
| GTCAACTGTTTGAATATTTG+GGG | 0.555701 | 7.4:-71614186 | MS.gene040366:CDS |
| CGCTGCAAAATGAAATGACT+AGG | 0.557513 | 7.4:+71614109 | None:intergenic |
| TCAAAACTTGAGGTGCATGC+TGG | 0.558360 | 7.4:-71614394 | MS.gene040366:CDS |
| AAGGAAGAAGGGATACCGAC+AGG | 0.558573 | 7.4:-71615348 | MS.gene040366:CDS |
| GATCAAGATTTAGCTATGAA+AGG | 0.561620 | 7.4:-71614877 | MS.gene040366:CDS |
| TATCAGAGCATCATGGATAC+CGG | 0.562826 | 7.4:-71614623 | MS.gene040366:CDS |
| AGTTGATCTTGCTGAAGACT+CGG | 0.563370 | 7.4:-71613966 | MS.gene040366:CDS |
| CAATGATCATAACCATGCTA+TGG | 0.573210 | 7.4:-71615448 | MS.gene040366:CDS |
| CAATCAAATTTATTTGTCAG+AGG | 0.576100 | 7.4:-71613822 | MS.gene040366:CDS |
| TACAACATGGTCAAAACTTG+AGG | 0.581192 | 7.4:-71614404 | MS.gene040366:CDS |
| CTTCCTTAAACTGTTCAACA+AGG | 0.583874 | 7.4:+71615364 | None:intergenic |
| CCACGCCAAGCACAAACCAA+AGG | 0.586137 | 7.4:-71613764 | MS.gene040366:CDS |
| TGATTATTCAATCCAACGAA+CGG | 0.587419 | 7.4:+71615021 | None:intergenic |
| TATATAGTATCAGAGCATCA+TGG | 0.587463 | 7.4:-71614630 | MS.gene040366:CDS |
| CAAAGGAAGAAAGAAGGATG+GGG | 0.594217 | 7.4:-71613747 | MS.gene040366:CDS |
| TACTGTTACAAGGGGTACAA+CGG | 0.611755 | 7.4:-71615499 | MS.gene040366:CDS |
| GCTTCACTTACTGTTACAAG+GGG | 0.617495 | 7.4:-71615507 | MS.gene040366:CDS |
| CAAAATTGCAGATTCAAGAG+TGG | 0.619428 | 7.4:-71613713 | MS.gene040366:CDS |
| TGTCATGAGTGTGGTGCACA+TGG | 0.635601 | 7.4:-71613647 | MS.gene040366:CDS |
| AGTAATGGATGCTAATGATG+TGG | 0.639172 | 7.4:-71615823 | None:intergenic |
| GGGATCTCCGAATGTTGGCG+AGG | 0.653176 | 7.4:-71615775 | MS.gene040366:CDS |
| TGTCAAAAGTTCTAACAACA+AGG | 0.663183 | 7.4:-71615592 | MS.gene040366:CDS |
| ACATCCTGGCTAGATTCCAA+AGG | 0.665285 | 7.4:+71613845 | None:intergenic |
| GTTACAAGGGGTACAACGGA+AGG | 0.671387 | 7.4:-71615495 | MS.gene040366:CDS |
| AAACTCGGAGGTTTACAACA+TGG | 0.673994 | 7.4:-71614417 | MS.gene040366:CDS |
| ATGAAAGTAAGCATAAAACT+CGG | 0.678069 | 7.4:-71614432 | MS.gene040366:CDS |
| TGGACTAAGGATGCTAACAA+AGG | 0.678940 | 7.4:-71614085 | MS.gene040366:CDS |
| ATTATTCAATCCAACGAACG+GGG | 0.683345 | 7.4:+71615023 | None:intergenic |
| AAAGTAAGCATAAAACTCGG+AGG | 0.714539 | 7.4:-71614429 | MS.gene040366:CDS |
| AGAAAAGCATGTCATGAGTG+TGG | 0.715894 | 7.4:-71613656 | MS.gene040366:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGTGTTAAAATTTGAAA+AGG | - | chr7.4:71614929-71614948 | MS.gene040366:CDS | 15.0% |
| !!! | AATTTATTTGCTAGAAAAAT+TGG | - | chr7.4:71613736-71613755 | MS.gene040366:CDS | 15.0% |
| !! | AATTCACTTCTATTATAAAC+CGG | + | chr7.4:71614822-71614841 | None:intergenic | 20.0% |
| !! | ATCTCATGAAAATATTTCAA+TGG | - | chr7.4:71613627-71613646 | MS.gene040366:CDS | 20.0% |
| !! | TCTCATGAAAATATTTCAAT+GGG | - | chr7.4:71613628-71613647 | MS.gene040366:CDS | 20.0% |
| !!! | CATTACCAATCAATTTTATT+TGG | - | chr7.4:71614423-71614442 | MS.gene040366:CDS | 20.0% |
| !!! | TCGAATGATAAATTTTTTCT+GGG | - | chr7.4:71614284-71614303 | MS.gene040366:CDS | 20.0% |
| !!! | TTGCTACAATTTTCAATAAT+GGG | - | chr7.4:71614103-71614122 | MS.gene040366:CDS | 20.0% |
| ! | AAGAAATCAAATTTACCTGT+TGG | - | chr7.4:71615542-71615561 | MS.gene040366:CDS | 25.0% |
| ! | AATAAATTTGATTGACATCC+TGG | + | chr7.4:71615595-71615614 | None:intergenic | 25.0% |
| ! | AGAAAGAAGATTAAAAGAGA+AGG | - | chr7.4:71615119-71615138 | MS.gene040366:CDS | 25.0% |
| ! | ATAATCTGGAAAACAATGAA+TGG | - | chr7.4:71614760-71614779 | MS.gene040366:CDS | 25.0% |
| ! | ATATGAAAAAACTTCGAAGT+AGG | - | chr7.4:71614160-71614179 | MS.gene040366:CDS | 25.0% |
| ! | ATGAAAGTAAGCATAAAACT+CGG | - | chr7.4:71614991-71615010 | MS.gene040366:CDS | 25.0% |
| ! | ATGAGATGTCATAAAAAGAT+GGG | - | chr7.4:71614021-71614040 | MS.gene040366:CDS | 25.0% |
| ! | ATGTCAACTGTTTGAATATT+TGG | - | chr7.4:71615235-71615254 | MS.gene040366:CDS | 25.0% |
| ! | CAATCAAATTTATTTGTCAG+AGG | - | chr7.4:71615601-71615620 | MS.gene040366:CDS | 25.0% |
| ! | CACAACCAAATAAAATTGAT+TGG | + | chr7.4:71614431-71614450 | None:intergenic | 25.0% |
| ! | GATATTGTGTAGACATATAT+TGG | - | chr7.4:71615259-71615278 | MS.gene040366:CDS | 25.0% |
| ! | TAAATAGTGAACCTTGTAAT+GGG | - | chr7.4:71613674-71613693 | MS.gene040366:CDS | 25.0% |
| ! | TATGAGATGTCATAAAAAGA+TGG | - | chr7.4:71614020-71614039 | MS.gene040366:CDS | 25.0% |
| ! | TGTCAACTGTTTGAATATTT+GGG | - | chr7.4:71615236-71615255 | MS.gene040366:CDS | 25.0% |
| ! | TTAAATAGTGAACCTTGTAA+TGG | - | chr7.4:71613673-71613692 | MS.gene040366:CDS | 25.0% |
| ! | TTTAATGGTTGAGTATAATC+TGG | - | chr7.4:71614746-71614765 | MS.gene040366:CDS | 25.0% |
| !! | GTTGCTACAATTTTCAATAA+TGG | - | chr7.4:71614102-71614121 | MS.gene040366:CDS | 25.0% |
| !! | TCATATAAGACACACTTTTA+GGG | + | chr7.4:71614005-71614024 | None:intergenic | 25.0% |
| !! | TGCTACAATTTTCAATAATG+GGG | - | chr7.4:71614104-71614123 | MS.gene040366:CDS | 25.0% |
| !!! | AATAGAAGTGAATTTTTTGC+TGG | - | chr7.4:71614828-71614847 | MS.gene040366:CDS | 25.0% |
| !!! | AGTAATTTTGATGATCAAAC+TGG | - | chr7.4:71615383-71615402 | MS.gene040366:CDS | 25.0% |
| !!! | ATAGAAGTGAATTTTTTGCT+GGG | - | chr7.4:71614829-71614848 | MS.gene040366:CDS | 25.0% |
| !!! | CTCGAATGATAAATTTTTTC+TGG | - | chr7.4:71614283-71614302 | MS.gene040366:CDS | 25.0% |
| !!! | GCACTTCAAAAATTTTAAGA+AGG | - | chr7.4:71615405-71615424 | MS.gene040366:CDS | 25.0% |
| !!! | TTTGCTAGAAAAATTGGTTT+TGG | - | chr7.4:71613742-71613761 | MS.gene040366:CDS | 25.0% |
| AAAACAATGAATGGCTTCAA+GGG | - | chr7.4:71614769-71614788 | MS.gene040366:CDS | 30.0% | |
| AAATGACATCTCCAAATTGT+TGG | + | chr7.4:71614335-71614354 | None:intergenic | 30.0% | |
| AGATTAGCATACCAACAATT+TGG | - | chr7.4:71614321-71614340 | MS.gene040366:CDS | 30.0% | |
| ATCTTGGTATGTTCTAATGA+AGG | - | chr7.4:71613799-71613818 | MS.gene040366:CDS | 30.0% | |
| GAACTTAAAAGTTGCATTCA+TGG | - | chr7.4:71614684-71614703 | MS.gene040366:CDS | 30.0% | |
| GATCAAGATTTAGCTATGAA+AGG | - | chr7.4:71614546-71614565 | MS.gene040366:CDS | 30.0% | |
| GTCAACTGTTTGAATATTTG+GGG | - | chr7.4:71615237-71615256 | MS.gene040366:CDS | 30.0% | |
| TAATTATTGCAAACAGAGAC+AGG | - | chr7.4:71614215-71614234 | MS.gene040366:CDS | 30.0% | |
| TACAATGTTTCGAAGATGAA+TGG | - | chr7.4:71614718-71614737 | MS.gene040366:CDS | 30.0% | |
| TCTTCGAAACATTGTATACT+CGG | + | chr7.4:71614714-71614733 | None:intergenic | 30.0% | |
| TGATCAGTTATGACAGATTT+GGG | + | chr7.4:71614531-71614550 | None:intergenic | 30.0% | |
| TGATTATTCAATCCAACGAA+CGG | + | chr7.4:71614405-71614424 | None:intergenic | 30.0% | |
| TTGAACAGTTTAAGGAAGAA+GGG | - | chr7.4:71614064-71614083 | MS.gene040366:CDS | 30.0% | |
| TTGATCAGTTATGACAGATT+TGG | + | chr7.4:71614532-71614551 | None:intergenic | 30.0% | |
| ! | CTCATATAAGACACACTTTT+AGG | + | chr7.4:71614006-71614025 | None:intergenic | 30.0% |
| ! | TAGAACTTTTGACAAGATGT+TGG | + | chr7.4:71613825-71613844 | None:intergenic | 30.0% |
| ! | TATATAGTATCAGAGCATCA+TGG | - | chr7.4:71614793-71614812 | MS.gene040366:CDS | 30.0% |
| ! | TGCTTTATTACAAGATGAGT+CGG | - | chr7.4:71614449-71614468 | MS.gene040366:CDS | 30.0% |
| ! | TGTCAAAAGTTCTAACAACA+AGG | - | chr7.4:71613831-71613850 | MS.gene040366:CDS | 30.0% |
| ! | TTGGTAATATTGCAAGCAAA+AGG | - | chr7.4:71615278-71615297 | MS.gene040366:CDS | 30.0% |
| ! | TTTAAGTTCTCGCTTGAAAA+TGG | + | chr7.4:71614673-71614692 | None:intergenic | 30.0% |
| !! | GTTGTTTGAAACTTTTCTTG+AGG | - | chr7.4:71614488-71614507 | MS.gene040366:CDS | 30.0% |
| !!! | GTACTTGTTGGTTTTATATG+TGG | + | chr7.4:71614367-71614386 | None:intergenic | 30.0% |
| !!! | TAAATATGAGCTAGCTTTTC+AGG | + | chr7.4:71614639-71614658 | None:intergenic | 30.0% |
| AAAACCGAAAAGAGCTATCT+TGG | - | chr7.4:71613783-71613802 | MS.gene040366:CDS | 35.0% | |
| AAAGTAAGCATAAAACTCGG+AGG | - | chr7.4:71614994-71615013 | MS.gene040366:CDS | 35.0% | |
| AAGCTTCACTTACTGTTACA+AGG | - | chr7.4:71613914-71613933 | MS.gene040366:CDS | 35.0% | |
| AATCCAAGATGACACAAATC+AGG | - | chr7.4:71613855-71613874 | MS.gene040366:CDS | 35.0% | |
| ACAAACCAAAGGAAGAAAGA+AGG | - | chr7.4:71615670-71615689 | MS.gene040366:CDS | 35.0% | |
| AGATGTCATAAAAAGATGGG+TGG | - | chr7.4:71614024-71614043 | MS.gene040366:CDS | 35.0% | |
| AGCTTCACTTACTGTTACAA+GGG | - | chr7.4:71613915-71613934 | MS.gene040366:CDS | 35.0% | |
| AGTAAGTGAAGCTTTACAAC+CGG | + | chr7.4:71613908-71613927 | None:intergenic | 35.0% | |
| ATTATTCAATCCAACGAACG+GGG | + | chr7.4:71614403-71614422 | None:intergenic | 35.0% | |
| CAAAATTGCAGATTCAAGAG+TGG | - | chr7.4:71615710-71615729 | MS.gene040366:CDS | 35.0% | |
| CAATGATCATAACCATGCTA+TGG | - | chr7.4:71613975-71613994 | MS.gene040366:CDS | 35.0% | |
| CGAAGTAGGAATTTAGATGT+TGG | - | chr7.4:71614174-71614193 | MS.gene040366:CDS | 35.0% | |
| CTTCCTTAAACTGTTCAACA+AGG | + | chr7.4:71614062-71614081 | None:intergenic | 35.0% | |
| CTTTCTCTAATAGGGATTGT+TGG | - | chr7.4:71614133-71614152 | MS.gene040366:CDS | 35.0% | |
| GAAAACAATGAATGGCTTCA+AGG | - | chr7.4:71614768-71614787 | MS.gene040366:CDS | 35.0% | |
| GACACAAAGATTGCTAAATG+TGG | - | chr7.4:71615212-71615231 | MS.gene040366:CDS | 35.0% | |
| GATTATTCAATCCAACGAAC+GGG | + | chr7.4:71614404-71614423 | None:intergenic | 35.0% | |
| GGATTCAGCTTTCTCTAATA+GGG | - | chr7.4:71614125-71614144 | MS.gene040366:CDS | 35.0% | |
| GTTGAACAGTTTAAGGAAGA+AGG | - | chr7.4:71614063-71614082 | MS.gene040366:CDS | 35.0% | |
| TACAACATGGTCAAAACTTG+AGG | - | chr7.4:71615019-71615038 | MS.gene040366:CDS | 35.0% | |
| TTGAATTCCATCCCATTACA+AGG | + | chr7.4:71613688-71613707 | None:intergenic | 35.0% | |
| TTTAATTCCTCGCCAACATT+CGG | + | chr7.4:71613658-71613677 | None:intergenic | 35.0% | |
| ! | ACTTTTCTTGAGGCAATGAA+TGG | - | chr7.4:71614498-71614517 | MS.gene040366:CDS | 35.0% |
| ! | AGTCGGAACGTTCTTTTATA+TGG | - | chr7.4:71614466-71614485 | MS.gene040366:CDS | 35.0% |
| ! | ATTGTAGCAACTTTTCCTGT+CGG | + | chr7.4:71614093-71614112 | None:intergenic | 35.0% |
| ! | CATACCAAGATAGCTCTTTT+CGG | + | chr7.4:71613790-71613809 | None:intergenic | 35.0% |
| ! | TCAAATTTACCTGTTGGTGA+TGG | - | chr7.4:71615548-71615567 | MS.gene040366:CDS | 35.0% |
| !! | AAGCCTTGTTGAACAGTTTA+AGG | - | chr7.4:71614056-71614075 | MS.gene040366:CDS | 35.0% |
| !! | AGATGAATGGAAGCGTTTAA+TGG | - | chr7.4:71614731-71614750 | MS.gene040366:CDS | 35.0% |
| AAACTCGGAGGTTTACAACA+TGG | - | chr7.4:71615006-71615025 | MS.gene040366:CDS | 40.0% | |
| AAGAAGGATGCATGTTCAAC+AGG | - | chr7.4:71615421-71615440 | MS.gene040366:CDS | 40.0% | |
| AAGAGTTCATCTTCTCGAAC+CGG | - | chr7.4:71613886-71613905 | MS.gene040366:CDS | 40.0% | |
| ACCAAAGGAAGAAAGAAGGA+TGG | - | chr7.4:71615674-71615693 | MS.gene040366:CDS | 40.0% | |
| AGTTGATCTTGCTGAAGACT+CGG | - | chr7.4:71615457-71615476 | MS.gene040366:CDS | 40.0% | |
| CAAAGGAAGAAAGAAGGATG+GGG | - | chr7.4:71615676-71615695 | MS.gene040366:CDS | 40.0% | |
| CCAAAGGAAGAAAGAAGGAT+GGG | - | chr7.4:71615675-71615694 | MS.gene040366:CDS | 40.0% | |
| CGCTGCAAAATGAAATGACT+AGG | + | chr7.4:71615317-71615336 | None:intergenic | 40.0% | |
| CTTAGCGGTAGAAAAAAGAG+AGG | - | chr7.4:71614965-71614984 | MS.gene040366:CDS | 40.0% | |
| GCTTCACTTACTGTTACAAG+GGG | - | chr7.4:71613916-71613935 | MS.gene040366:CDS | 40.0% | |
| GGGATTCAGCTTTCTCTAAT+AGG | - | chr7.4:71614124-71614143 | MS.gene040366:CDS | 40.0% | |
| TGGACTAAGGATGCTAACAA+AGG | - | chr7.4:71615338-71615357 | MS.gene040366:CDS | 40.0% | |
| TTCGAGAATCTTCATCGCAT+TGG | + | chr7.4:71614269-71614288 | None:intergenic | 40.0% | |
| ! | AAAGGGCATACTGTACTTGT+TGG | + | chr7.4:71614379-71614398 | None:intergenic | 40.0% |
| ! | AGAAAAGCATGTCATGAGTG+TGG | - | chr7.4:71615767-71615786 | MS.gene040366:CDS | 40.0% |
| ! | GTCATTTCATTTTGCAGCGT+TGG | - | chr7.4:71615318-71615337 | MS.gene040366:CDS | 40.0% |
| ! | TACTGTTACAAGGGGTACAA+CGG | - | chr7.4:71613924-71613943 | MS.gene040366:CDS | 40.0% |
| ! | TAGCCTGATTTGTGTCATCT+TGG | + | chr7.4:71613861-71613880 | None:intergenic | 40.0% |
| ! | TAGTGAACCTTGTAATGGGA+TGG | - | chr7.4:71613678-71613697 | MS.gene040366:CDS | 40.0% |
| ! | TATCAGAGCATCATGGATAC+CGG | - | chr7.4:71614800-71614819 | MS.gene040366:CDS | 40.0% |
| !! | TGGTGATGGAATTTCTCCTT+TGG | - | chr7.4:71615562-71615581 | MS.gene040366:CDS | 40.0% |
| !! | TTCTTCCTTTGGTTTGTGCT+TGG | + | chr7.4:71615667-71615686 | None:intergenic | 40.0% |
| !!! | CTCTTTTTTCTACCGCTAAG+CGG | + | chr7.4:71614965-71614984 | None:intergenic | 40.0% |
| !!! | TTTTAGGGCTAACCATAGCA+TGG | + | chr7.4:71613990-71614009 | None:intergenic | 40.0% |
| ACATCCTGGCTAGATTCCAA+AGG | + | chr7.4:71615581-71615600 | None:intergenic | 45.0% | |
| CCCATCCTTCTTTCTTCCTT+TGG | + | chr7.4:71615678-71615697 | None:intergenic | 45.0% | |
| GAACAGCCAGAACTCACTAT+TGG | - | chr7.4:71615632-71615651 | MS.gene040366:CDS | 45.0% | |
| GGAGAAATTCCATCACCAAC+AGG | + | chr7.4:71615560-71615579 | None:intergenic | 45.0% | |
| GGGTTTCCAATAGTGAGTTC+TGG | + | chr7.4:71615641-71615660 | None:intergenic | 45.0% | |
| TCAAAACTTGAGGTGCATGC+TGG | - | chr7.4:71615029-71615048 | MS.gene040366:CDS | 45.0% | |
| TCAATGGGATCTCCGAATGT+TGG | - | chr7.4:71613643-71613662 | MS.gene040366:CDS | 45.0% | |
| TCTTGCAAGTTCTGCTGTTG+TGG | + | chr7.4:71615802-71615821 | None:intergenic | 45.0% | |
| !! | CATTTTGCAGCGTTGGACTA+AGG | - | chr7.4:71615325-71615344 | MS.gene040366:CDS | 45.0% |
| !! | TTCTCCTTTGGAATCTAGCC+AGG | - | chr7.4:71615574-71615593 | MS.gene040366:CDS | 45.0% |
| AAGGAAGAAGGGATACCGAC+AGG | - | chr7.4:71614075-71614094 | MS.gene040366:CDS | 50.0% | |
| AATCCAACGAACGGGGCAAA+GGG | + | chr7.4:71614396-71614415 | None:intergenic | 50.0% | |
| GCCTCTGCTTGTGGCATATT+CGG | - | chr7.4:71614604-71614623 | MS.gene040366:CDS | 50.0% | |
| GGGGTACAACGGAAGGTAAT+TGG | - | chr7.4:71613935-71613954 | MS.gene040366:CDS | 50.0% | |
| TCCGAATATGCCACAAGCAG+AGG | + | chr7.4:71614608-71614627 | None:intergenic | 50.0% | |
| TGTCATGAGTGTGGTGCACA+TGG | - | chr7.4:71615776-71615795 | MS.gene040366:CDS | 50.0% | |
| ! | CAGAGGCGATGTCTAGTTTC+TGG | + | chr7.4:71614591-71614610 | None:intergenic | 50.0% |
| ! | GTTACAAGGGGTACAACGGA+AGG | - | chr7.4:71613928-71613947 | MS.gene040366:CDS | 50.0% |
| !! | CTTTGGTTTGTGCTTGGCGT+GGG | + | chr7.4:71615661-71615680 | None:intergenic | 50.0% |
| CAATCCAACGAACGGGGCAA+AGG | + | chr7.4:71614397-71614416 | None:intergenic | 55.0% | |
| CCACGCCAAGCACAAACCAA+AGG | - | chr7.4:71615659-71615678 | MS.gene040366:CDS | 55.0% | |
| CTAGACATCGCCTCTGCTTG+TGG | - | chr7.4:71614595-71614614 | MS.gene040366:CDS | 55.0% | |
| !! | CCTTTGGTTTGTGCTTGGCG+TGG | + | chr7.4:71615662-71615681 | None:intergenic | 55.0% |
| ATGCCCTTTGCCCCGTTCGT+TGG | - | chr7.4:71614390-71614409 | MS.gene040366:CDS | 60.0% | |
| GGGATCTCCGAATGTTGGCG+AGG | - | chr7.4:71613648-71613667 | MS.gene040366:CDS | 60.0% | |
| ! | GGCGCTTGATAGCCGCTTAG+CGG | - | chr7.4:71614950-71614969 | MS.gene040366:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 71613604 | 71615841 | 71613604 | ID=MS.gene040366 |
| chr7.4 | mRNA | 71613604 | 71615841 | 71613604 | ID=MS.gene040366.t1;Parent=MS.gene040366 |
| chr7.4 | exon | 71613604 | 71615841 | 71613604 | ID=MS.gene040366.t1.exon1;Parent=MS.gene040366.t1 |
| chr7.4 | CDS | 71613604 | 71615841 | 71613604 | ID=cds.MS.gene040366.t1;Parent=MS.gene040366.t1 |
| Gene Sequence |
| Protein sequence |
