Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040369.t1 | TKY49529.1 | 30 | 160 | 99 | 1 | 148 | 307 | 59 | 205 | 8.40E-10 | 74.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040369.t1 | B9S8R1 | 26.0 | 173 | 121 | 3 | 123 | 294 | 311 | 477 | 2.8e-07 | 65.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene040369.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 57 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCAGCATGATCATAATTT+TGG | 0.162734 | 7.4:-71661278 | None:intergenic |
| CCATAAGAGTTGCAATTAAA+AGG | 0.192720 | 7.4:+71661449 | MS.gene040369:CDS |
| GTAAATAAATCCATTCATTT+TGG | 0.238833 | 7.4:-71660873 | None:intergenic |
| CCTGGAATGGGGTATATTAT+TGG | 0.269750 | 7.4:+71660406 | MS.gene040369:CDS |
| AGCATTATTCTGTCTGAAAT+TGG | 0.307236 | 7.4:-71660623 | None:intergenic |
| ATTGGAGCAATAAACCCTTT+TGG | 0.316443 | 7.4:-71660605 | None:intergenic |
| TTTCATCCCATCCTTTGTTT+AGG | 0.324262 | 7.4:-71660895 | None:intergenic |
| CCATTTCTTTCAGATTGTTT+CGG | 0.339635 | 7.4:-71661390 | None:intergenic |
| TGAATATCCAACAAGTTATC+TGG | 0.341722 | 7.4:+71660577 | MS.gene040369:CDS |
| TTATTATGTCCCCATTTGTT+AGG | 0.355324 | 7.4:-71660364 | None:intergenic |
| CATTCTTCCACATCCCCATT+TGG | 0.364629 | 7.4:-71661298 | None:intergenic |
| GATGCAGAAGTAATTATATC+AGG | 0.367565 | 7.4:+71661010 | MS.gene040369:CDS |
| TCTGAAAGAAATGGATGAAC+TGG | 0.387878 | 7.4:+71661399 | MS.gene040369:CDS |
| ATGAAACACAACCTAACAAA+TGG | 0.402473 | 7.4:+71660353 | MS.gene040369:CDS |
| TCCATCCAACACCAAATGAT+AGG | 0.403265 | 7.4:-71660445 | None:intergenic |
| CCGAAACAATCTGAAAGAAA+TGG | 0.409508 | 7.4:+71661390 | MS.gene040369:CDS |
| CCAATAATATACCCCATTCC+AGG | 0.420327 | 7.4:-71660406 | None:intergenic |
| ATTATGATCATGCTGCCAAA+TGG | 0.431314 | 7.4:+71661283 | MS.gene040369:CDS |
| GATAGAGGAACCCTATCATT+TGG | 0.440970 | 7.4:+71660434 | MS.gene040369:CDS |
| TGAAACACAACCTAACAAAT+GGG | 0.442644 | 7.4:+71660354 | MS.gene040369:CDS |
| TTATGATCATGCTGCCAAAT+GGG | 0.444203 | 7.4:+71661284 | MS.gene040369:CDS |
| AACATGGGATAACCTGGAAT+GGG | 0.457967 | 7.4:+71660394 | MS.gene040369:CDS |
| CCCTATCATTTGGTGTTGGA+TGG | 0.460015 | 7.4:+71660444 | MS.gene040369:CDS |
| CATATATCATTTAGATGAGC+TGG | 0.461622 | 7.4:-71660782 | None:intergenic |
| AGTTATCTGGAATGTCCAAA+AGG | 0.467450 | 7.4:+71660590 | MS.gene040369:CDS |
| CATAAGAGTTGCAATTAAAA+GGG | 0.481340 | 7.4:+71661450 | MS.gene040369:CDS |
| ACATACCAGAGGTTGTCTTT+CGG | 0.481848 | 7.4:-71661125 | None:intergenic |
| GGACATTCCAGATAACTTGT+TGG | 0.497580 | 7.4:-71660584 | None:intergenic |
| TTATTTACCTAAACAAAGGA+TGG | 0.508173 | 7.4:+71660888 | MS.gene040369:CDS |
| TTCCACCGAAAGACAACCTC+TGG | 0.516844 | 7.4:+71661120 | MS.gene040369:CDS |
| GAACATGGGATAACCTGGAA+TGG | 0.517331 | 7.4:+71660393 | MS.gene040369:CDS |
| TATCAGAACATGGGATAACC+TGG | 0.522187 | 7.4:+71660388 | MS.gene040369:CDS |
| AAAATCAAATTGAAATTATG+GGG | 0.522382 | 7.4:+71660819 | MS.gene040369:CDS |
| GTTGAGTGGACCAAAATGAA+TGG | 0.523563 | 7.4:+71660863 | MS.gene040369:CDS |
| GTTATCTGGAATGTCCAAAA+GGG | 0.527940 | 7.4:+71660591 | MS.gene040369:CDS |
| GGAACCCTATCATTTGGTGT+TGG | 0.543877 | 7.4:+71660440 | MS.gene040369:CDS |
| TATTTACCTAAACAAAGGAT+GGG | 0.547487 | 7.4:+71660889 | MS.gene040369:CDS |
| GCTATCGTGCTTGAACAATA+CGG | 0.548281 | 7.4:+71660506 | MS.gene040369:CDS |
| GATTTATCTGGATCGTTGAG+TGG | 0.560786 | 7.4:+71660849 | MS.gene040369:CDS |
| TACCAGAGGTTGTCTTTCGG+TGG | 0.564724 | 7.4:-71661122 | None:intergenic |
| CATGCTGCCAAATGGGGATG+TGG | 0.565724 | 7.4:+71661291 | MS.gene040369:CDS |
| CCATCCAACACCAAATGATA+GGG | 0.567379 | 7.4:-71660444 | None:intergenic |
| ATATTATTGGAAGAAGATAG+AGG | 0.568423 | 7.4:+71660419 | MS.gene040369:CDS |
| GGATTTATTTACCTAAACAA+AGG | 0.571285 | 7.4:+71660884 | MS.gene040369:CDS |
| CAAATGGGGATGTGGAAGAA+TGG | 0.573013 | 7.4:+71661299 | MS.gene040369:CDS |
| AAATGTTACGAATGTCGCCT+AGG | 0.574831 | 7.4:+71661343 | MS.gene040369:CDS |
| TCAGAATATTCCAGTTCAGG+TGG | 0.575638 | 7.4:+71661228 | MS.gene040369:intron |
| CAGAATAATGCTACACACAC+TGG | 0.585878 | 7.4:+71660634 | MS.gene040369:CDS |
| ACATAATAACTATCAGAACA+TGG | 0.590614 | 7.4:+71660378 | MS.gene040369:CDS |
| TGGATATTCAGTGTACATCA+TGG | 0.607172 | 7.4:-71660564 | None:intergenic |
| ATCGCATAGTTCTATTGACA+AGG | 0.612868 | 7.4:-71660927 | None:intergenic |
| CATAATAACTATCAGAACAT+GGG | 0.626665 | 7.4:+71660379 | MS.gene040369:CDS |
| TATGATCATGCTGCCAAATG+GGG | 0.628423 | 7.4:+71661285 | MS.gene040369:CDS |
| GTTGATGTAAATGGTTCACA+AGG | 0.652594 | 7.4:-71661476 | None:intergenic |
| AAATCAAATTGAAATTATGG+GGG | 0.660572 | 7.4:+71660820 | MS.gene040369:CDS |
| GAAACACAACCTAACAAATG+GGG | 0.672406 | 7.4:+71660355 | MS.gene040369:CDS |
| ACATGGGATAACCTGGAATG+GGG | 0.681669 | 7.4:+71660395 | MS.gene040369:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATCAAATTGAAATTAT+GGG | + | chr7.4:71660818-71660837 | MS.gene040369:CDS | 10.0% |
| !! | AAAATCAAATTGAAATTATG+GGG | + | chr7.4:71660819-71660838 | MS.gene040369:CDS | 15.0% |
| !! | CAAAAATCAAATTGAAATTA+TGG | + | chr7.4:71660817-71660836 | MS.gene040369:CDS | 15.0% |
| !! | AAATCAAATTGAAATTATGG+GGG | + | chr7.4:71660820-71660839 | MS.gene040369:CDS | 20.0% |
| !! | AAATTTAATATACATACCAG+AGG | - | chr7.4:71661139-71661158 | None:intergenic | 20.0% |
| !! | TCTGGTATGTATATTAAATT+TGG | + | chr7.4:71661138-71661157 | MS.gene040369:intron | 20.0% |
| !!! | ATTTAAAGTAGACAATTAAC+AGG | - | chr7.4:71660749-71660768 | None:intergenic | 20.0% |
| !!! | GTAAATAAATCCATTCATTT+TGG | - | chr7.4:71660876-71660895 | None:intergenic | 20.0% |
| ! | ACATAATAACTATCAGAACA+TGG | + | chr7.4:71660378-71660397 | MS.gene040369:CDS | 25.0% |
| ! | ATATTATTGGAAGAAGATAG+AGG | + | chr7.4:71660419-71660438 | MS.gene040369:CDS | 25.0% |
| ! | CATAAGAGTTGCAATTAAAA+GGG | + | chr7.4:71661450-71661469 | MS.gene040369:CDS | 25.0% |
| ! | CATAATAACTATCAGAACAT+GGG | + | chr7.4:71660379-71660398 | MS.gene040369:CDS | 25.0% |
| ! | GGATTTATTTACCTAAACAA+AGG | + | chr7.4:71660884-71660903 | MS.gene040369:CDS | 25.0% |
| ! | GTATGTATATTAAATTTGGC+AGG | + | chr7.4:71661142-71661161 | MS.gene040369:intron | 25.0% |
| ! | TATTTACCTAAACAAAGGAT+GGG | + | chr7.4:71660889-71660908 | MS.gene040369:CDS | 25.0% |
| ! | TTATTTACCTAAACAAAGGA+TGG | + | chr7.4:71660888-71660907 | MS.gene040369:CDS | 25.0% |
| !! | AATAATTTTCGCAATGATAC+TGG | + | chr7.4:71661037-71661056 | MS.gene040369:CDS | 25.0% |
| !! | ATAATTTTCGCAATGATACT+GGG | + | chr7.4:71661038-71661057 | MS.gene040369:CDS | 25.0% |
| !!! | CAACTTTTTTTCATGTGATT+CGG | - | chr7.4:71661074-71661093 | None:intergenic | 25.0% |
| ATGAAACACAACCTAACAAA+TGG | + | chr7.4:71660353-71660372 | MS.gene040369:CDS | 30.0% | |
| ATTGAAATTATGGGGGAAAA+TGG | + | chr7.4:71660827-71660846 | MS.gene040369:CDS | 30.0% | |
| CATATATCATTTAGATGAGC+TGG | - | chr7.4:71660785-71660804 | None:intergenic | 30.0% | |
| CCATAAGAGTTGCAATTAAA+AGG | + | chr7.4:71661449-71661468 | MS.gene040369:CDS | 30.0% | |
| CCATTTCTTTCAGATTGTTT+CGG | - | chr7.4:71661393-71661412 | None:intergenic | 30.0% | |
| GATGCAGAAGTAATTATATC+AGG | + | chr7.4:71661010-71661029 | MS.gene040369:CDS | 30.0% | |
| GGAAAAAGATGGTATAAGTT+TGG | + | chr7.4:71661364-71661383 | MS.gene040369:CDS | 30.0% | |
| TGAAACACAACCTAACAAAT+GGG | + | chr7.4:71660354-71660373 | MS.gene040369:CDS | 30.0% | |
| TGAATATCCAACAAGTTATC+TGG | + | chr7.4:71660577-71660596 | MS.gene040369:CDS | 30.0% | |
| TTATTATGTCCCCATTTGTT+AGG | - | chr7.4:71660367-71660386 | None:intergenic | 30.0% | |
| ! | AACTGGAGTTTTACAAAACA+GGG | + | chr7.4:71661416-71661435 | MS.gene040369:CDS | 30.0% |
| ! | ACTTATACCATCTTTTTCCT+AGG | - | chr7.4:71661363-71661382 | None:intergenic | 30.0% |
| ! | AGCATTATTCTGTCTGAAAT+TGG | - | chr7.4:71660626-71660645 | None:intergenic | 30.0% |
| ! | TTTTCAGAATATTCCAGTTC+AGG | + | chr7.4:71661225-71661244 | MS.gene040369:intron | 30.0% |
| !! | CCTTTTAATTGCAACTCTTA+TGG | - | chr7.4:71661452-71661471 | None:intergenic | 30.0% |
| !!! | TTTTTTTCATGTGATTCGGT+TGG | - | chr7.4:71661070-71661089 | None:intergenic | 30.0% |
| ATTATGATCATGCTGCCAAA+TGG | + | chr7.4:71661283-71661302 | MS.gene040369:CDS | 35.0% | |
| CCGAAACAATCTGAAAGAAA+TGG | + | chr7.4:71661390-71661409 | MS.gene040369:CDS | 35.0% | |
| GAAACACAACCTAACAAATG+GGG | + | chr7.4:71660355-71660374 | MS.gene040369:CDS | 35.0% | |
| GACAATAATAAGTTGTGCTG+CGG | - | chr7.4:71660666-71660685 | None:intergenic | 35.0% | |
| TCTGAAAGAAATGGATGAAC+TGG | + | chr7.4:71661399-71661418 | MS.gene040369:CDS | 35.0% | |
| TGGATATTCAGTGTACATCA+TGG | - | chr7.4:71660567-71660586 | None:intergenic | 35.0% | |
| TTATGATCATGCTGCCAAAT+GGG | + | chr7.4:71661284-71661303 | MS.gene040369:CDS | 35.0% | |
| ! | ACTTGATGTTGTGAGTTTTG+TGG | - | chr7.4:71661101-71661120 | None:intergenic | 35.0% |
| ! | AGTTATCTGGAATGTCCAAA+AGG | + | chr7.4:71660590-71660609 | MS.gene040369:CDS | 35.0% |
| ! | ATCGCATAGTTCTATTGACA+AGG | - | chr7.4:71660930-71660949 | None:intergenic | 35.0% |
| ! | ATTGGAGCAATAAACCCTTT+TGG | - | chr7.4:71660608-71660627 | None:intergenic | 35.0% |
| ! | GAACTGGAGTTTTACAAAAC+AGG | + | chr7.4:71661415-71661434 | MS.gene040369:CDS | 35.0% |
| ! | GTTATCTGGAATGTCCAAAA+GGG | + | chr7.4:71660591-71660610 | MS.gene040369:CDS | 35.0% |
| ! | TGGCAGCATGATCATAATTT+TGG | - | chr7.4:71661281-71661300 | None:intergenic | 35.0% |
| ! | TTTCATCCCATCCTTTGTTT+AGG | - | chr7.4:71660898-71660917 | None:intergenic | 35.0% |
| !! | AGCATGATCATAATTTTGGC+TGG | - | chr7.4:71661277-71661296 | None:intergenic | 35.0% |
| !!! | CATAATTTTGGCTGGTTTGT+CGG | - | chr7.4:71661269-71661288 | None:intergenic | 35.0% |
| AAATGTTACGAATGTCGCCT+AGG | + | chr7.4:71661343-71661362 | MS.gene040369:CDS | 40.0% | |
| AACATGGGATAACCTGGAAT+GGG | + | chr7.4:71660394-71660413 | MS.gene040369:CDS | 40.0% | |
| AATGTCGCCTAGGAAAAAGA+TGG | + | chr7.4:71661353-71661372 | MS.gene040369:CDS | 40.0% | |
| ACATACCAGAGGTTGTCTTT+CGG | - | chr7.4:71661128-71661147 | None:intergenic | 40.0% | |
| CAGAATAATGCTACACACAC+TGG | + | chr7.4:71660634-71660653 | MS.gene040369:CDS | 40.0% | |
| CCAATAATATACCCCATTCC+AGG | - | chr7.4:71660409-71660428 | None:intergenic | 40.0% | |
| CCATCCAACACCAAATGATA+GGG | - | chr7.4:71660447-71660466 | None:intergenic | 40.0% | |
| CCTGGAATGGGGTATATTAT+TGG | + | chr7.4:71660406-71660425 | MS.gene040369:CDS | 40.0% | |
| GATAGAGGAACCCTATCATT+TGG | + | chr7.4:71660434-71660453 | MS.gene040369:CDS | 40.0% | |
| GATTTATCTGGATCGTTGAG+TGG | + | chr7.4:71660849-71660868 | MS.gene040369:CDS | 40.0% | |
| GCTATCGTGCTTGAACAATA+CGG | + | chr7.4:71660506-71660525 | MS.gene040369:CDS | 40.0% | |
| GTTGAGTGGACCAAAATGAA+TGG | + | chr7.4:71660863-71660882 | MS.gene040369:CDS | 40.0% | |
| TATCAGAACATGGGATAACC+TGG | + | chr7.4:71660388-71660407 | MS.gene040369:CDS | 40.0% | |
| TATGATCATGCTGCCAAATG+GGG | + | chr7.4:71661285-71661304 | MS.gene040369:CDS | 40.0% | |
| TCAGAATATTCCAGTTCAGG+TGG | + | chr7.4:71661228-71661247 | MS.gene040369:intron | 40.0% | |
| TCCATCCAACACCAAATGAT+AGG | - | chr7.4:71660448-71660467 | None:intergenic | 40.0% | |
| ! | GGACATTCCAGATAACTTGT+TGG | - | chr7.4:71660587-71660606 | None:intergenic | 40.0% |
| ! | TGGGGGAAAATGGATTTATC+TGG | + | chr7.4:71660837-71660856 | MS.gene040369:CDS | 40.0% |
| ACATGGGATAACCTGGAATG+GGG | + | chr7.4:71660395-71660414 | MS.gene040369:CDS | 45.0% | |
| CAAATGGGGATGTGGAAGAA+TGG | + | chr7.4:71661299-71661318 | MS.gene040369:CDS | 45.0% | |
| CATTCTTCCACATCCCCATT+TGG | - | chr7.4:71661301-71661320 | None:intergenic | 45.0% | |
| GAACATGGGATAACCTGGAA+TGG | + | chr7.4:71660393-71660412 | MS.gene040369:CDS | 45.0% | |
| ! | AAACGTTTTGCCACCTGAAC+TGG | - | chr7.4:71661241-71661260 | None:intergenic | 45.0% |
| !! | CCCTATCATTTGGTGTTGGA+TGG | + | chr7.4:71660444-71660463 | MS.gene040369:CDS | 45.0% |
| !! | GGAACCCTATCATTTGGTGT+TGG | + | chr7.4:71660440-71660459 | MS.gene040369:CDS | 45.0% |
| TACCAGAGGTTGTCTTTCGG+TGG | - | chr7.4:71661125-71661144 | None:intergenic | 50.0% | |
| TTCCACCGAAAGACAACCTC+TGG | + | chr7.4:71661120-71661139 | MS.gene040369:CDS | 50.0% | |
| CATGCTGCCAAATGGGGATG+TGG | + | chr7.4:71661291-71661310 | MS.gene040369:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 71660335 | 71661483 | 71660335 | ID=MS.gene040369 |
| chr7.4 | mRNA | 71660335 | 71661483 | 71660335 | ID=MS.gene040369.t1;Parent=MS.gene040369 |
| chr7.4 | exon | 71660335 | 71660655 | 71660335 | ID=MS.gene040369.t1.exon1;Parent=MS.gene040369.t1 |
| chr7.4 | CDS | 71660335 | 71660655 | 71660335 | ID=cds.MS.gene040369.t1;Parent=MS.gene040369.t1 |
| chr7.4 | exon | 71660776 | 71661141 | 71660776 | ID=MS.gene040369.t1.exon2;Parent=MS.gene040369.t1 |
| chr7.4 | CDS | 71660776 | 71661141 | 71660776 | ID=cds.MS.gene040369.t1;Parent=MS.gene040369.t1 |
| chr7.4 | exon | 71661232 | 71661483 | 71661232 | ID=MS.gene040369.t1.exon3;Parent=MS.gene040369.t1 |
| chr7.4 | CDS | 71661232 | 71661483 | 71661232 | ID=cds.MS.gene040369.t1;Parent=MS.gene040369.t1 |
| Gene Sequence |
| Protein sequence |
