Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040395.t1 | RHN49639.1 | 67.7 | 158 | 28 | 4 | 54 | 189 | 10 | 166 | 1.80E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040395.t1 | A0A396H8N5 | 67.7 | 158 | 28 | 4 | 54 | 189 | 10 | 166 | 1.3e-47 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene040395.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040395.t1 | MTR_7g117590 | 68.387 | 155 | 27 | 4 | 56 | 189 | 18 | 171 | 1.16e-63 | 194 |
| MS.gene040395.t1 | MTR_1g054470 | 48.108 | 185 | 36 | 6 | 1 | 179 | 3 | 133 | 1.33e-45 | 147 |
| MS.gene040395.t1 | MTR_2g012700 | 37.273 | 110 | 68 | 1 | 82 | 190 | 11 | 120 | 1.56e-27 | 100 |
| MS.gene040395.t1 | MTR_2g062330 | 39.316 | 117 | 66 | 3 | 75 | 190 | 27 | 139 | 3.55e-26 | 98.2 |
| MS.gene040395.t1 | MTR_0010s0310 | 33.333 | 90 | 57 | 1 | 94 | 180 | 151 | 240 | 3.84e-13 | 66.2 |
| MS.gene040395.t1 | MTR_5g071400 | 68.085 | 47 | 8 | 3 | 56 | 96 | 14 | 59 | 6.99e-12 | 59.7 |
| MS.gene040395.t1 | MTR_6g477950 | 36.275 | 102 | 61 | 3 | 89 | 189 | 46 | 144 | 8.77e-12 | 62.8 |
| MS.gene040395.t1 | MTR_3g008190 | 28.571 | 98 | 69 | 1 | 95 | 191 | 6 | 103 | 3.35e-11 | 58.2 |
| MS.gene040395.t1 | MTR_6g087720 | 32.039 | 103 | 66 | 3 | 79 | 177 | 196 | 298 | 5.02e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGATGGTTTGAGTTTGTT+AGG | 0.273672 | 7.2:-68533718 | MS.gene040395:CDS |
| TGAAGATCGGGTCATTTGAT+TGG | 0.369943 | 7.2:-68534399 | MS.gene040395:CDS |
| CGAATTGTTGATGATGAAAC+TGG | 0.373851 | 7.2:-68533800 | MS.gene040395:CDS |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 7.2:-68533767 | MS.gene040395:CDS |
| GGCTGCTGGACGGAGAGTTC+TGG | 0.386406 | 7.2:-68533985 | MS.gene040395:intron |
| TGGCCATCGACTTCTTCATT+TGG | 0.387700 | 7.2:+68534080 | None:intergenic |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 7.2:-68533760 | MS.gene040395:CDS |
| TTTGAAATTGTGCAAATACT+TGG | 0.402550 | 7.2:+68534060 | None:intergenic |
| ATTAGTTCTGCTACGGCTGC+TGG | 0.408831 | 7.2:-68533999 | MS.gene040395:CDS |
| GAAGATCGGGTCATTTGATT+GGG | 0.425740 | 7.2:-68534398 | MS.gene040395:CDS |
| CGAAGAAGAAGTTGAAGATC+GGG | 0.445939 | 7.2:-68534411 | MS.gene040395:CDS |
| ATGAAATGTGCGGCGGATGC+AGG | 0.449012 | 7.2:-68534462 | MS.gene040395:CDS |
| TGGATGGTTTGAGTTTGTTA+GGG | 0.484898 | 7.2:-68533717 | MS.gene040395:CDS |
| CTGCAGCGATGTAATTGATC+CGG | 0.509939 | 7.2:-68534135 | MS.gene040395:intron |
| CTTCTTCGTCCATCTCAAAA+CGG | 0.535091 | 7.2:+68534426 | None:intergenic |
| ACGAAGAAGAAGTTGAAGAT+CGG | 0.540825 | 7.2:-68534412 | MS.gene040395:CDS |
| CGATGTAATTGATCCGGAGT+TGG | 0.544205 | 7.2:-68534129 | MS.gene040395:CDS |
| CAATCTTATTGCCGTCAAAA+CGG | 0.544280 | 7.2:+68534366 | None:intergenic |
| TTATTGCCGTCAAAACGGTT+CGG | 0.545486 | 7.2:+68534371 | None:intergenic |
| CTTTGAATTGCAATCTTCCG+AGG | 0.557056 | 7.2:+68533848 | None:intergenic |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 7.2:-68533756 | MS.gene040395:CDS |
| GGTTTCAATTCGTCCAACTC+CGG | 0.567151 | 7.2:+68534116 | None:intergenic |
| AGAAATACGTTGGAAGTGGA+TGG | 0.575514 | 7.2:-68533733 | MS.gene040395:CDS |
| ATGGAGAAATACGTTGGAAG+TGG | 0.576439 | 7.2:-68533737 | MS.gene040395:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 7.2:-68534006 | MS.gene040395:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 7.2:-68533743 | MS.gene040395:CDS |
| CCATCTCAAAACGGCGACGA+TGG | 0.584489 | 7.2:+68534435 | None:intergenic |
| AGGGCAAAAGAACTCAAAGA+AGG | 0.584629 | 7.2:-68533698 | MS.gene040395:CDS |
| TTAATGTGTAGCGTATTCCT+CGG | 0.586314 | 7.2:-68533865 | MS.gene040395:intron |
| GCATCCGCCGCACATTTCAT+CGG | 0.590420 | 7.2:+68534465 | None:intergenic |
| GATACAAATAATTTGTGTGG+AGG | 0.593345 | 7.2:+68533647 | None:intergenic |
| ACTGATACAAATAATTTGTG+TGG | 0.607437 | 7.2:+68533644 | None:intergenic |
| GTTCTGCTACGGCTGCTGGA+CGG | 0.628877 | 7.2:-68533995 | MS.gene040395:CDS |
| AATCCAAATGAAGAAGTCGA+TGG | 0.671453 | 7.2:-68534083 | MS.gene040395:CDS |
| ATGGCGACCGATGAAATGTG+CGG | 0.726557 | 7.2:-68534472 | MS.gene040395:CDS |
| GCGACCGATGAAATGTGCGG+CGG | 0.753822 | 7.2:-68534469 | MS.gene040395:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTTCAAAATTTTCAAAA+CGG | - | chr7.2:68534056-68534075 | MS.gene040395:CDS | 15.0% |
| !!! | GTTGTTTTTTTTTAATCATT+TGG | - | chr7.2:68533922-68533941 | MS.gene040395:intron | 15.0% |
| !!! | GTTTTTTATTTTATTTTTGC+TGG | - | chr7.2:68534162-68534181 | MS.gene040395:intron | 15.0% |
| ! | CTTAAAATAGCCTTCTAAAT+GGG | + | chr7.2:68533805-68533824 | None:intergenic | 25.0% |
| !! | ACTGATACAAATAATTTGTG+TGG | + | chr7.2:68534462-68534481 | None:intergenic | 25.0% |
| !! | TTTGAAATTGTGCAAATACT+TGG | + | chr7.2:68534046-68534065 | None:intergenic | 25.0% |
| !!! | CATTTAGAAGGCTATTTTAA+GGG | - | chr7.2:68533804-68533823 | MS.gene040395:CDS | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | - | chr7.2:68534343-68534362 | MS.gene040395:CDS | 30.0% | |
| CCTTAAAATAGCCTTCTAAA+TGG | + | chr7.2:68533806-68533825 | None:intergenic | 30.0% | |
| ! | AAAACGGTGATCATACTTTT+TGG | - | chr7.2:68534072-68534091 | MS.gene040395:CDS | 30.0% |
| ! | GATACAAATAATTTGTGTGG+AGG | + | chr7.2:68534459-68534478 | None:intergenic | 30.0% |
| !! | CCATTTAGAAGGCTATTTTA+AGG | - | chr7.2:68533803-68533822 | MS.gene040395:CDS | 30.0% |
| !!! | AAGATTGGGCTTTTTAGTTA+TGG | - | chr7.2:68533753-68533772 | MS.gene040395:CDS | 30.0% |
| AATCCAAATGAAGAAGTCGA+TGG | - | chr7.2:68534020-68534039 | MS.gene040395:CDS | 35.0% | |
| AATTGGATGGAGAAATACGT+TGG | - | chr7.2:68534360-68534379 | MS.gene040395:CDS | 35.0% | |
| ACGAAGAAGAAGTTGAAGAT+CGG | - | chr7.2:68533691-68533710 | MS.gene040395:CDS | 35.0% | |
| ACGTCTGAGAAGAAAATTCT+AGG | - | chr7.2:68533851-68533870 | MS.gene040395:CDS | 35.0% | |
| CAATCTTATTGCCGTCAAAA+CGG | + | chr7.2:68533740-68533759 | None:intergenic | 35.0% | |
| CGTCTGAGAAGAAAATTCTA+GGG | - | chr7.2:68533852-68533871 | MS.gene040395:CDS | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | - | chr7.2:68534336-68534355 | MS.gene040395:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | - | chr7.2:68534347-68534366 | MS.gene040395:CDS | 35.0% | |
| TGAAAATGTCTTCTTCTAGC+AGG | - | chr7.2:68533883-68533902 | MS.gene040395:intron | 35.0% | |
| TTAAGGGTTGTTGTGAATCT+TGG | - | chr7.2:68533820-68533839 | MS.gene040395:CDS | 35.0% | |
| TTAATGTGTAGCGTATTCCT+CGG | - | chr7.2:68534238-68534257 | MS.gene040395:intron | 35.0% | |
| ! | TCATTTGGATTTTCGTCTAC+AGG | + | chr7.2:68534011-68534030 | None:intergenic | 35.0% |
| !! | CGAATTGTTGATGATGAAAC+TGG | - | chr7.2:68534303-68534322 | MS.gene040395:intron | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | - | chr7.2:68534097-68534116 | MS.gene040395:CDS | 35.0% |
| !! | GTTTTGACGGCAATAAGATT+GGG | - | chr7.2:68533739-68533758 | MS.gene040395:CDS | 35.0% |
| !! | TGGATGGTTTGAGTTTGTTA+GGG | - | chr7.2:68534386-68534405 | MS.gene040395:CDS | 35.0% |
| AGAAATACGTTGGAAGTGGA+TGG | - | chr7.2:68534370-68534389 | MS.gene040395:CDS | 40.0% | |
| AGGGCAAAAGAACTCAAAGA+AGG | - | chr7.2:68534405-68534424 | MS.gene040395:CDS | 40.0% | |
| ATGGAGAAATACGTTGGAAG+TGG | - | chr7.2:68534366-68534385 | MS.gene040395:CDS | 40.0% | |
| CGAAGAAGAAGTTGAAGATC+GGG | - | chr7.2:68533692-68533711 | MS.gene040395:CDS | 40.0% | |
| CTTCTTCGTCCATCTCAAAA+CGG | + | chr7.2:68533680-68533699 | None:intergenic | 40.0% | |
| CTTTGAATTGCAATCTTCCG+AGG | + | chr7.2:68534258-68534277 | None:intergenic | 40.0% | |
| GAAGATCGGGTCATTTGATT+GGG | - | chr7.2:68533705-68533724 | MS.gene040395:CDS | 40.0% | |
| TGAAGATCGGGTCATTTGAT+TGG | - | chr7.2:68533704-68533723 | MS.gene040395:CDS | 40.0% | |
| TTATTGCCGTCAAAACGGTT+CGG | + | chr7.2:68533735-68533754 | None:intergenic | 40.0% | |
| ! | GTGGATGGTTTGAGTTTGTT+AGG | - | chr7.2:68534385-68534404 | MS.gene040395:CDS | 40.0% |
| ! | TTGCTGGAGTATTTGCACAT+GGG | - | chr7.2:68534178-68534197 | MS.gene040395:intron | 40.0% |
| ! | TTTGCTGGAGTATTTGCACA+TGG | - | chr7.2:68534177-68534196 | MS.gene040395:intron | 40.0% |
| !! | CGTTTTGACGGCAATAAGAT+TGG | - | chr7.2:68533738-68533757 | MS.gene040395:CDS | 40.0% |
| !!! | TGGGCTTTTTAGTTATGGAC+CGG | - | chr7.2:68533758-68533777 | MS.gene040395:CDS | 40.0% |
| ATGGACCGGGCAAAAATGAA+TGG | - | chr7.2:68533772-68533791 | MS.gene040395:CDS | 45.0% | |
| CGATGTAATTGATCCGGAGT+TGG | - | chr7.2:68533974-68533993 | MS.gene040395:intron | 45.0% | |
| CTGCAGCGATGTAATTGATC+CGG | - | chr7.2:68533968-68533987 | MS.gene040395:intron | 45.0% | |
| GGGCAAAAATGAATGGGTCA+CGG | - | chr7.2:68533779-68533798 | MS.gene040395:CDS | 45.0% | |
| GGTTTCAATTCGTCCAACTC+CGG | + | chr7.2:68533990-68534009 | None:intergenic | 45.0% | |
| TGGACCGGGCAAAAATGAAT+GGG | - | chr7.2:68533773-68533792 | MS.gene040395:CDS | 45.0% | |
| ! | GGTTTTCCGAACCGTTTTGA+CGG | - | chr7.2:68533726-68533745 | MS.gene040395:CDS | 45.0% |
| !! | GTGACCCATTCATTTTTGCC+CGG | + | chr7.2:68533780-68533799 | None:intergenic | 45.0% |
| !! | TGGCCATCGACTTCTTCATT+TGG | + | chr7.2:68534026-68534045 | None:intergenic | 45.0% |
| !!! | GGGCTTTTTAGTTATGGACC+GGG | - | chr7.2:68533759-68533778 | MS.gene040395:CDS | 45.0% |
| ATGGCGACCGATGAAATGTG+CGG | - | chr7.2:68533631-68533650 | MS.gene040395:CDS | 50.0% | |
| GAGTATTTGCACATGGGTGG+TGG | - | chr7.2:68534184-68534203 | MS.gene040395:intron | 50.0% | |
| ! | CTGGAGTATTTGCACATGGG+TGG | - | chr7.2:68534181-68534200 | MS.gene040395:intron | 50.0% |
| !! | ATTAGTTCTGCTACGGCTGC+TGG | - | chr7.2:68534104-68534123 | MS.gene040395:CDS | 50.0% |
| ATGAAATGTGCGGCGGATGC+AGG | - | chr7.2:68533641-68533660 | MS.gene040395:CDS | 55.0% | |
| CCATCTCAAAACGGCGACGA+TGG | + | chr7.2:68533671-68533690 | None:intergenic | 55.0% | |
| GCATCCGCCGCACATTTCAT+CGG | + | chr7.2:68533641-68533660 | None:intergenic | 55.0% | |
| TGGGTCACGGCCCATTTAGA+AGG | - | chr7.2:68533792-68533811 | MS.gene040395:CDS | 55.0% | |
| !!! | CCATCGTCGCCGTTTTGAGA+TGG | - | chr7.2:68533668-68533687 | MS.gene040395:CDS | 55.0% |
| GCGACCGATGAAATGTGCGG+CGG | - | chr7.2:68533634-68533653 | MS.gene040395:CDS | 60.0% | |
| GTTCTGCTACGGCTGCTGGA+CGG | - | chr7.2:68534108-68534127 | MS.gene040395:CDS | 60.0% | |
| ! | GCACATGGGTGGTGGCTTTC+TGG | - | chr7.2:68534192-68534211 | MS.gene040395:intron | 60.0% |
| ! | GCTGGACGGAGAGTTCTGGT+TGG | - | chr7.2:68534122-68534141 | MS.gene040395:CDS | 60.0% |
| GGCTGCTGGACGGAGAGTTC+TGG | - | chr7.2:68534118-68534137 | MS.gene040395:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 68533631 | 68534494 | 68533631 | ID=MS.gene040395 |
| chr7.2 | mRNA | 68533631 | 68534494 | 68533631 | ID=MS.gene040395.t1;Parent=MS.gene040395 |
| chr7.2 | exon | 68534331 | 68534494 | 68534331 | ID=MS.gene040395.t1.exon1;Parent=MS.gene040395.t1 |
| chr7.2 | CDS | 68534331 | 68534494 | 68534331 | ID=cds.MS.gene040395.t1;Parent=MS.gene040395.t1 |
| chr7.2 | exon | 68533986 | 68534151 | 68533986 | ID=MS.gene040395.t1.exon2;Parent=MS.gene040395.t1 |
| chr7.2 | CDS | 68533986 | 68534151 | 68533986 | ID=cds.MS.gene040395.t1;Parent=MS.gene040395.t1 |
| chr7.2 | exon | 68533631 | 68533876 | 68533631 | ID=MS.gene040395.t1.exon3;Parent=MS.gene040395.t1 |
| chr7.2 | CDS | 68533631 | 68533876 | 68533631 | ID=cds.MS.gene040395.t1;Parent=MS.gene040395.t1 |
| Gene Sequence |
| Protein sequence |
