Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040473.t1 | XP_013447966.1 | 89.1 | 220 | 24 | 0 | 1 | 220 | 1 | 220 | 1.20E-98 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040473.t1 | Q39261 | 42.1 | 76 | 37 | 3 | 83 | 151 | 52 | 127 | 1.4e-07 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040473.t1 | A0A072TYR6 | 89.1 | 220 | 24 | 0 | 1 | 220 | 1 | 220 | 8.4e-99 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene040473.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040473.t1 | MTR_7g028890 | 89.091 | 220 | 24 | 0 | 1 | 220 | 1 | 220 | 1.44e-143 | 399 |
| MS.gene040473.t1 | MTR_7g022250 | 74.477 | 239 | 42 | 2 | 1 | 220 | 1 | 239 | 7.46e-126 | 355 |
| MS.gene040473.t1 | MTR_7g028915 | 71.368 | 234 | 31 | 2 | 1 | 220 | 1 | 212 | 8.21e-112 | 319 |
| MS.gene040473.t1 | MTR_7g022270 | 58.791 | 182 | 53 | 4 | 20 | 200 | 1 | 161 | 2.33e-69 | 209 |
| MS.gene040473.t1 | MTR_4g128340 | 30.714 | 140 | 78 | 4 | 83 | 220 | 47 | 169 | 1.20e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040473.t1 | AT5G57520 | 34.579 | 107 | 54 | 5 | 82 | 179 | 51 | 150 | 3.06e-11 | 59.7 |
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTGGATATCAATATTA+TGG | 0.285744 | 7.3:+71529576 | MS.gene040473:CDS |
| AAGAAATGGAGAAATTTCTT+TGG | 0.293893 | 7.3:-71529370 | None:intergenic |
| CTTAAGGGTCAAATCAATAT+TGG | 0.328703 | 7.3:-71529826 | None:intergenic |
| CTCCTTCCTCTTGTTGCACA+TGG | 0.338528 | 7.3:-71529190 | None:intergenic |
| TATATATAAATCAAAGCTTA+AGG | 0.356106 | 7.3:-71529842 | None:intergenic |
| TGGTGCATTGCAACATATTC+CGG | 0.362580 | 7.3:+71529656 | MS.gene040473:CDS |
| CACCATCACTCCATTTCATA+TGG | 0.364608 | 7.3:+71529554 | MS.gene040473:CDS |
| AACAATATGATTCAACATGC+AGG | 0.449556 | 7.3:+71529603 | MS.gene040473:CDS |
| TATCCACACCCCATATGAAA+TGG | 0.452870 | 7.3:-71529564 | None:intergenic |
| AGTAGACAATTTGAAGATGA+TGG | 0.466141 | 7.3:+71529230 | MS.gene040473:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7.3:+71529465 | MS.gene040473:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7.3:+71529397 | MS.gene040473:CDS |
| TGAAGTTGGTAAAGTTTCAG+AGG | 0.484639 | 7.3:+71529743 | MS.gene040473:CDS |
| ACTATGTCAAAGATTGAAGT+TGG | 0.488225 | 7.3:+71529729 | MS.gene040473:CDS |
| ACCATCACTCCATTTCATAT+GGG | 0.489513 | 7.3:+71529555 | MS.gene040473:CDS |
| TGGTGTTTAGACTTATCCCT+TGG | 0.491881 | 7.3:-71529536 | None:intergenic |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7.3:+71529773 | MS.gene040473:CDS |
| CAAGCGAAAGGCAATTGTGC+TGG | 0.510108 | 7.3:+71529272 | MS.gene040473:CDS |
| GAAGTTGGTAAAGTTTCAGA+GGG | 0.513957 | 7.3:+71529744 | MS.gene040473:CDS |
| ATATATAAATCAAAGCTTAA+GGG | 0.520066 | 7.3:-71529841 | None:intergenic |
| ACTCCATTTCATATGGGGTG+TGG | 0.533278 | 7.3:+71529561 | MS.gene040473:CDS |
| TCATTCGTTTCATCTATCTC+CGG | 0.537068 | 7.3:-71529675 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7.3:+71529458 | MS.gene040473:CDS |
| ACAATATGATTCAACATGCA+GGG | 0.581032 | 7.3:+71529604 | MS.gene040473:CDS |
| CTGATGCCATGTGCAACAAG+AGG | 0.598560 | 7.3:+71529184 | None:intergenic |
| TAGAATTCTGAGTTTCAACA+TGG | 0.602831 | 7.3:-71529302 | None:intergenic |
| TGCCATGTGCAACAAGAGGA+AGG | 0.602996 | 7.3:+71529188 | None:intergenic |
| GGAGATAGATGAAACGAATG+AGG | 0.622863 | 7.3:+71529677 | MS.gene040473:CDS |
| GTTGGTAAAGTTTCAGAGGG+AGG | 0.624522 | 7.3:+71529747 | MS.gene040473:CDS |
| CCATCACTCCATTTCATATG+GGG | 0.630153 | 7.3:+71529556 | MS.gene040473:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7.3:+71529794 | MS.gene040473:CDS |
| GTGCAACAAGAGGAAGGAGA+AGG | 0.635140 | 7.3:+71529194 | MS.gene040473:CDS |
| CCCCATATGAAATGGAGTGA+TGG | 0.640487 | 7.3:-71529556 | None:intergenic |
| GAACAATGAGAACAAGCGAA+AGG | 0.645636 | 7.3:+71529260 | MS.gene040473:CDS |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7.3:+71529468 | MS.gene040473:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.664402 | 7.3:-71529474 | None:intergenic |
| AGAATTCTGAGTTTCAACAT+GGG | 0.673130 | 7.3:-71529301 | None:intergenic |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7.3:+71529797 | MS.gene040473:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | - | chr7.3:71529373-71529392 | None:intergenic | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.3:71529387-71529406 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | + | chr7.3:71529378-71529397 | MS.gene040473:CDS | 25.0% |
| !! | TTTTATGCAGGAAATTTGTA+TGG | + | chr7.3:71529636-71529655 | MS.gene040473:CDS | 25.0% |
| AACAATATGATTCAACATGC+AGG | + | chr7.3:71529603-71529622 | MS.gene040473:CDS | 30.0% | |
| ACAATATGATTCAACATGCA+GGG | + | chr7.3:71529604-71529623 | MS.gene040473:CDS | 30.0% | |
| ACTATGTCAAAGATTGAAGT+TGG | + | chr7.3:71529729-71529748 | MS.gene040473:CDS | 30.0% | |
| AGAATTCTGAGTTTCAACAT+GGG | - | chr7.3:71529304-71529323 | None:intergenic | 30.0% | |
| AGTAGACAATTTGAAGATGA+TGG | + | chr7.3:71529230-71529249 | MS.gene040473:CDS | 30.0% | |
| AGTCTCAATAAAAAGATCCA+AGG | + | chr7.3:71529519-71529538 | MS.gene040473:CDS | 30.0% | |
| CTTAAGGGTCAAATCAATAT+TGG | - | chr7.3:71529829-71529848 | None:intergenic | 30.0% | |
| GTCTCAATAAAAAGATCCAA+GGG | + | chr7.3:71529520-71529539 | MS.gene040473:CDS | 30.0% | |
| TAGAATTCTGAGTTTCAACA+TGG | - | chr7.3:71529305-71529324 | None:intergenic | 30.0% | |
| ACCATCACTCCATTTCATAT+GGG | + | chr7.3:71529555-71529574 | MS.gene040473:CDS | 35.0% | |
| GGGTGTGGATATCAATATTA+TGG | + | chr7.3:71529576-71529595 | MS.gene040473:CDS | 35.0% | |
| TCATTCGTTTCATCTATCTC+CGG | - | chr7.3:71529678-71529697 | None:intergenic | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | + | chr7.3:71529397-71529416 | MS.gene040473:CDS | 35.0% | |
| ! | CTTCTTCAGCAACATCTTTT+TGG | - | chr7.3:71529335-71529354 | None:intergenic | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | + | chr7.3:71529773-71529792 | MS.gene040473:CDS | 35.0% |
| !! | GAAGTTGGTAAAGTTTCAGA+GGG | + | chr7.3:71529744-71529763 | MS.gene040473:CDS | 35.0% |
| !! | TGAAGTTGGTAAAGTTTCAG+AGG | + | chr7.3:71529743-71529762 | MS.gene040473:CDS | 35.0% |
| CACCATCACTCCATTTCATA+TGG | + | chr7.3:71529554-71529573 | MS.gene040473:CDS | 40.0% | |
| CCATCACTCCATTTCATATG+GGG | + | chr7.3:71529556-71529575 | MS.gene040473:CDS | 40.0% | |
| GAACAATGAGAACAAGCGAA+AGG | + | chr7.3:71529260-71529279 | MS.gene040473:CDS | 40.0% | |
| GGAGATAGATGAAACGAATG+AGG | + | chr7.3:71529677-71529696 | MS.gene040473:CDS | 40.0% | |
| TATCCACACCCCATATGAAA+TGG | - | chr7.3:71529567-71529586 | None:intergenic | 40.0% | |
| TGGTGCATTGCAACATATTC+CGG | + | chr7.3:71529656-71529675 | MS.gene040473:CDS | 40.0% | |
| TGGTGTTTAGACTTATCCCT+TGG | - | chr7.3:71529539-71529558 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7.3:71529624-71529643 | MS.gene040473:CDS | 40.0% |
| ACTCCATTTCATATGGGGTG+TGG | + | chr7.3:71529561-71529580 | MS.gene040473:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | + | chr7.3:71529797-71529816 | MS.gene040473:CDS | 45.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | + | chr7.3:71529458-71529477 | MS.gene040473:CDS | 45.0% | |
| CCCCATATGAAATGGAGTGA+TGG | - | chr7.3:71529559-71529578 | None:intergenic | 45.0% | |
| GGAAGACAAAGAGACTCAAG+AGG | + | chr7.3:71529794-71529813 | MS.gene040473:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | + | chr7.3:71529465-71529484 | MS.gene040473:CDS | 45.0% | |
| ! | GTTGGTAAAGTTTCAGAGGG+AGG | + | chr7.3:71529747-71529766 | MS.gene040473:CDS | 45.0% |
| ACTAGTCCACAGGCATTAGG+TGG | + | chr7.3:71529468-71529487 | MS.gene040473:CDS | 50.0% | |
| CAAGCGAAAGGCAATTGTGC+TGG | + | chr7.3:71529272-71529291 | MS.gene040473:CDS | 50.0% | |
| GTGCAACAAGAGGAAGGAGA+AGG | + | chr7.3:71529194-71529213 | MS.gene040473:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | - | chr7.3:71529477-71529496 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 71529192 | 71529854 | 71529192 | ID=MS.gene040473 |
| chr7.3 | mRNA | 71529192 | 71529854 | 71529192 | ID=MS.gene040473.t1;Parent=MS.gene040473 |
| chr7.3 | exon | 71529192 | 71529854 | 71529192 | ID=MS.gene040473.t1.exon1;Parent=MS.gene040473.t1 |
| chr7.3 | CDS | 71529192 | 71529854 | 71529192 | ID=cds.MS.gene040473.t1;Parent=MS.gene040473.t1 |
| Gene Sequence |
| Protein sequence |
