AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040652

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040652.t1 MTR_1g050502 79.911 224 29 2 1 224 1 208 4.49e-123 349
MS.gene040652.t1 MTR_1g050502 87.374 198 9 2 1 198 1 182 1.37e-119 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040652.t1 AT1G42990 56.250 64 27 1 136 198 155 218 4.22e-14 71.2

Find 74 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCCCTGCTATGGTTCC+TGG 0.181734 1.2:+33500066 MS.gene040652:CDS
GAGAGATTCGAAATCGATTT+GGG 0.252626 1.2:-33498010 None:intergenic
CTAAGAAGGGTGGCTCTAAA+AGG 0.288472 1.2:+33500172 MS.gene040652:CDS
AATACCTACTCTTCGTTTCA+AGG 0.332279 1.2:-33498661 None:intergenic
AGAGAGATTCGAAATCGATT+TGG 0.334822 1.2:-33498011 None:intergenic
GAAGGCTCTTGAGGAGTTAC+TGG 0.356888 1.2:-33498276 None:intergenic
TGTTGCAGAATCCCTGCTGC+TGG 0.361805 1.2:+33500045 MS.gene040652:intron
GGAGTCTGCTGTGCTCTTGT+TGG 0.367032 1.2:+33498799 MS.gene040652:CDS
TGAAGGGGAATGCAGAAGAT+TGG 0.381118 1.2:+33498685 MS.gene040652:CDS
GGAACCTCTTGAGGAGTTAC+TGG 0.381189 1.2:-33498321 None:intergenic
TCAGAGATATTATTACTGTT+TGG 0.385576 1.2:-33500196 None:intergenic
TTGCAATCGCGTGGTGGCTT+TGG 0.414223 1.2:+33498758 MS.gene040652:CDS
TTTCAGGCAGATGAGGAATA+GGG 0.426727 1.2:+33498592 MS.gene040652:intron
GGTTCCCTGCTATGGTTCCT+GGG 0.429306 1.2:+33500067 MS.gene040652:CDS
CCTCGACCCGATCCCTCAAT+CGG 0.430109 1.2:+33498212 MS.gene040652:CDS
TGCTGCTGGGTTCCCTGCTA+TGG 0.441437 1.2:+33500059 MS.gene040652:CDS
GAGTCTGCTGTGCTCTTGTT+GGG 0.447976 1.2:+33498800 MS.gene040652:CDS
TGATCATCCGATTGAGGGAT+CGG 0.459847 1.2:-33498219 None:intergenic
AGCCTTCTTCTGATGAAGCC+AGG 0.459858 1.2:+33498292 MS.gene040652:CDS
TGGTGCCCAGGAACCATAGC+AGG 0.462764 1.2:-33500072 None:intergenic
AGCAGACTCCTGCATGGTCA+TGG 0.499805 1.2:-33498786 None:intergenic
ATAGCAGGGAACCCAGCAGC+AGG 0.506596 1.2:-33500057 None:intergenic
TCTTTGAATGATAGCAACAT+TGG 0.506755 1.2:-33499945 None:intergenic
AACCTTGATCATCCGATTGA+GGG 0.507174 1.2:-33498224 None:intergenic
GGGAATTGCGTCGTCATCAT+CGG 0.507358 1.2:+33498155 MS.gene040652:CDS
ATGCTGAGAACCATGCTTTG+CGG 0.510527 1.2:+33498729 MS.gene040652:CDS
AAACCTTGATCATCCGATTG+AGG 0.512112 1.2:-33498225 None:intergenic
CCGATTGAGGGATCGGGTCG+AGG 0.513783 1.2:-33498212 None:intergenic
CTGTTGTTAGCCACAGTGGA+AGG 0.514860 1.2:-33500114 None:intergenic
TGGAACACAAAGGTCTAAGA+AGG 0.519321 1.2:+33500158 MS.gene040652:CDS
TGGTGCTCCCATGACCATGC+AGG 0.523079 1.2:+33498778 MS.gene040652:CDS
TCATCAGAAGAAGGCTCTTG+AGG 0.530695 1.2:-33498285 None:intergenic
GCCTTCTTCTGATGAAGCCA+GGG 0.533209 1.2:+33498293 MS.gene040652:CDS
GTTGCAGAATCCCTGCTGCT+GGG 0.537879 1.2:+33500046 MS.gene040652:intron
TCTTCTCATGTCTGATGCTG+AGG 0.542387 1.2:+33498134 MS.gene040652:CDS
AGGAGTTACTGGATCAACTC+TGG 0.544068 1.2:-33498265 None:intergenic
TGAGTTGGCACATGGTGCCC+AGG 0.544147 1.2:-33500084 None:intergenic
GATTCTCAATCTCTGAGAGA+GGG 0.544535 1.2:-33498113 None:intergenic
GTTGTTAGCCACAGTGGAAG+GGG 0.545341 1.2:-33500112 None:intergenic
ATCCCTCAATCGGATGATCA+AGG 0.545906 1.2:+33498222 MS.gene040652:CDS
TTCGAGAAACTTATGATAGT+CGG 0.546362 1.2:-33498181 None:intergenic
TCAACTCTGGCTTCATCAGA+AGG 0.547608 1.2:-33498252 None:intergenic
ACCCTGGCTTCATCAGAAGA+AGG 0.549174 1.2:-33498294 None:intergenic
CTTTGTTTGCAATCGCGTGG+TGG 0.550397 1.2:+33498752 MS.gene040652:CDS
TAAGAAGGGTGGCTCTAAAA+GGG 0.555483 1.2:+33500173 MS.gene040652:CDS
GCAGACTCCTGCATGGTCAT+GGG 0.561161 1.2:-33498785 None:intergenic
TCAATCTCTGAGAGAGGGGA+GGG 0.571872 1.2:-33498108 None:intergenic
GCAAACAAAGCCGCAAAGCA+TGG 0.573484 1.2:-33498739 None:intergenic
GATCATCCGATTGAGGGATC+GGG 0.580413 1.2:-33498218 None:intergenic
TGTTGTTAGCCACAGTGGAA+GGG 0.582265 1.2:-33500113 None:intergenic
CTCAATCTCTGAGAGAGGGG+AGG 0.584965 1.2:-33498109 None:intergenic
GGTTCAGGAGGAACCTCTTG+AGG 0.589392 1.2:-33498330 None:intergenic
GTTACCTCCAAGAGAAAACA+TGG 0.596124 1.2:+33500138 MS.gene040652:CDS
AGATTCTCAATCTCTGAGAG+AGG 0.602579 1.2:-33498114 None:intergenic
CTTCTCATGTCTGATGCTGA+GGG 0.604464 1.2:+33498135 MS.gene040652:CDS
GTGGAAGGGGTAGACTGAGT+TGG 0.606292 1.2:-33500099 None:intergenic
AGGAGTTACTGGATCAACCC+TGG 0.607536 1.2:-33498310 None:intergenic
CGGCTTTGTTTGCAATCGCG+TGG 0.608761 1.2:+33498749 MS.gene040652:CDS
GTGTCTGCGACGGAAACAGA+AGG 0.621465 1.2:-33498084 None:intergenic
ATTCTCAATCTCTGAGAGAG+GGG 0.625653 1.2:-33498112 None:intergenic
GGGTAGACTGAGTTGGCACA+TGG 0.627667 1.2:-33500092 None:intergenic
AACACTGTTGTTAGCCACAG+TGG 0.628137 1.2:-33500118 None:intergenic
AGGGGAGGGAGTGTCTGCGA+CGG 0.631896 1.2:-33498094 None:intergenic
TGATCCAGTAACTCCTCAAG+AGG 0.636811 1.2:+33498317 MS.gene040652:CDS
CTGCTGTGAAATCAAGAGAG+AGG 0.642211 1.2:+33498618 MS.gene040652:CDS
AGAGATTCGAAATCGATTTG+GGG 0.646761 1.2:-33498009 None:intergenic
AAAACCTTGAAACGAAGAGT+AGG 0.647793 1.2:+33498657 MS.gene040652:CDS
TAGCAGGGAACCCAGCAGCA+GGG 0.658619 1.2:-33500056 None:intergenic
GGAACACAAAGGTCTAAGAA+GGG 0.664648 1.2:+33500159 MS.gene040652:CDS
ACACAAAGGTCTAAGAAGGG+TGG 0.678689 1.2:+33500162 MS.gene040652:CDS
GAGCACAGCAGACTCCTGCA+TGG 0.688063 1.2:-33498792 None:intergenic
GGTGCCCAGGAACCATAGCA+GGG 0.695738 1.2:-33500071 None:intergenic
AGAGAAAACATGGAACACAA+AGG 0.721160 1.2:+33500148 MS.gene040652:CDS
TCAGTCTACCCCTTCCACTG+TGG 0.742334 1.2:+33500104 MS.gene040652:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAAATACGAAAAATAAAA+TGG - chr1.2:33498880-33498899 None:intergenic 15.0%
!! ATCTAATGATTTATGTTTCA+TGG - chr1.2:33499426-33499445 None:intergenic 20.0%
!! ATTCATTTAACATGCATAAA+GGG - chr1.2:33499900-33499919 None:intergenic 20.0%
!! TAAATGGAAAAAATTTGTGT+GGG + chr1.2:33499681-33499700 MS.gene040652:intron 20.0%
!! TACATAAAACATATACAACA+TGG + chr1.2:33499842-33499861 MS.gene040652:intron 20.0%
!! TATTCATTTAACATGCATAA+AGG - chr1.2:33499901-33499920 None:intergenic 20.0%
!! TCTAATGATTTATGTTTCAT+GGG - chr1.2:33499425-33499444 None:intergenic 20.0%
!!! AATCATTAGATCTTTTTAGT+TGG + chr1.2:33499435-33499454 MS.gene040652:intron 20.0%
!!! TAATTTGTAATTTGTATTCG+AGG + chr1.2:33498504-33498523 MS.gene040652:intron 20.0%
! ACGATAATCATTAGAGATTA+AGG - chr1.2:33499080-33499099 None:intergenic 25.0%
! AGAGATTAAGGTTAAAACTT+GGG - chr1.2:33499068-33499087 None:intergenic 25.0%
! CTAAATGGAAAAAATTTGTG+TGG + chr1.2:33499680-33499699 MS.gene040652:intron 25.0%
! TACATAAAGCTCAATAACTA+TGG - chr1.2:33499565-33499584 None:intergenic 25.0%
! TAGAGATTAAGGTTAAAACT+TGG - chr1.2:33499069-33499088 None:intergenic 25.0%
! TTACAGAATATGATCAACAT+TGG - chr1.2:33498404-33498423 None:intergenic 25.0%
!! GAAAGTAGGCAAATTATTTT+AGG - chr1.2:33498984-33499003 None:intergenic 25.0%
!!! CCTAATTAGCTTTTTTGAAA+CGG - chr1.2:33498355-33498374 None:intergenic 25.0%
!!! CTAATTAGCTTTTTTGAAAC+GGG - chr1.2:33498354-33498373 None:intergenic 25.0%
!!! TTTAGGTTTTTGCATCTAAA+TGG + chr1.2:33499665-33499684 MS.gene040652:intron 25.0%
!!! TTTTTTGGTATCTGATTTTC+AGG + chr1.2:33498576-33498595 MS.gene040652:intron 25.0%
AAATTACAAATTACTCCAGC+AGG - chr1.2:33498497-33498516 None:intergenic 30.0%
AATAAAATGGCAACGACAAT+AGG - chr1.2:33498867-33498886 None:intergenic 30.0%
ATAATATTTCACCTTCTCAC+CGG + chr1.2:33499532-33499551 MS.gene040652:intron 30.0%
ATCCATTCATTCAAGAAAGT+AGG - chr1.2:33498998-33499017 None:intergenic 30.0%
CCGTTTCAAAAAAGCTAATT+AGG + chr1.2:33498352-33498371 MS.gene040652:CDS 30.0%
TCTTTGAATGATAGCAACAT+TGG - chr1.2:33499948-33499967 None:intergenic 30.0%
TTCGAGAAACTTATGATAGT+CGG - chr1.2:33498184-33498203 None:intergenic 30.0%
! AAAAATTTGTGTGGGTTGTA+TGG + chr1.2:33499689-33499708 MS.gene040652:intron 30.0%
! ATACATTACCACTGTTGTTT+AGG + chr1.2:33499648-33499667 MS.gene040652:intron 30.0%
! ATTGAGCTTTATGTATGTAC+TGG + chr1.2:33499570-33499589 MS.gene040652:intron 30.0%
! CTAAAAGTATAACCATATGG+AGG + chr1.2:33498908-33498927 MS.gene040652:intron 30.0%
! CTGCTAAAAGTATAACCATA+TGG + chr1.2:33498905-33498924 MS.gene040652:intron 30.0%
!!! ACGTCTTTAGGTTTTTTGTT+GGG + chr1.2:33499199-33499218 MS.gene040652:intron 30.0%
!!! CTTTTTAGTTGGAGTATCTT+AGG + chr1.2:33499446-33499465 MS.gene040652:intron 30.0%
!!! GGTTCTAAAGAGAAGTTTTT+TGG + chr1.2:33498561-33498580 MS.gene040652:intron 30.0%
!!! TACTTTTTCTCTGAGAGTTT+TGG - chr1.2:33499873-33499892 None:intergenic 30.0%
AAAACCTTGAAACGAAGAGT+AGG + chr1.2:33498657-33498676 MS.gene040652:CDS 35.0%
AATACCTACTCTTCGTTTCA+AGG - chr1.2:33498664-33498683 None:intergenic 35.0%
AGAGAAAACATGGAACACAA+AGG + chr1.2:33500148-33500167 MS.gene040652:CDS 35.0%
AGAGAGATTCGAAATCGATT+TGG - chr1.2:33498014-33498033 None:intergenic 35.0%
AGCAACTATGCTATGTTATC+TGG + chr1.2:33499620-33499639 MS.gene040652:intron 35.0%
ATTTCAGATCTCATCTCATG+CGG - chr1.2:33499371-33499390 None:intergenic 35.0%
CAAATCTGGATTACAGCAAA+AGG - chr1.2:33499807-33499826 None:intergenic 35.0%
CAATAACTATGGTGATAGAC+CGG - chr1.2:33499554-33499573 None:intergenic 35.0%
CACCTTTGGACCAAAATAAA+AGG - chr1.2:33499779-33499798 None:intergenic 35.0%
CAGATGTTAAAGACGTCTTT+AGG + chr1.2:33499187-33499206 MS.gene040652:intron 35.0%
GACCTCAAAAACACTATAGT+GGG - chr1.2:33499600-33499619 None:intergenic 35.0%
GCAACTATGCTATGTTATCT+GGG + chr1.2:33499621-33499640 MS.gene040652:intron 35.0%
TGACCTCAAAAACACTATAG+TGG - chr1.2:33499601-33499620 None:intergenic 35.0%
TGCAAAAACCTAAACAACAG+TGG - chr1.2:33499659-33499678 None:intergenic 35.0%
TGCCTACTTTCTTGAATGAA+TGG + chr1.2:33498993-33499012 MS.gene040652:intron 35.0%
TGCTCTATAGTTTCATCTGT+CGG + chr1.2:33499028-33499047 MS.gene040652:intron 35.0%
TTTGCAGAACACAAAGAACT+AGG + chr1.2:33499223-33499242 MS.gene040652:intron 35.0%
TTTGCATAAGCACACAAATC+TGG - chr1.2:33499821-33499840 None:intergenic 35.0%
! GAGAGATTCGAAATCGATTT+GGG - chr1.2:33498013-33498032 None:intergenic 35.0%
! GTTTTATGAAGCTGCTCTAA+TGG + chr1.2:33499972-33499991 MS.gene040652:intron 35.0%
! TTGTGTTCCATGTTTTCTCT+TGG - chr1.2:33500148-33500167 None:intergenic 35.0%
!! ACGAAGAGTAGGTATTTTGA+AGG + chr1.2:33498668-33498687 MS.gene040652:CDS 35.0%
!! AGAGATTCGAAATCGATTTG+GGG - chr1.2:33498012-33498031 None:intergenic 35.0%
!! ATCCCACTATAGTGTTTTTG+AGG + chr1.2:33499595-33499614 MS.gene040652:intron 35.0%
!! CGAAGAGTAGGTATTTTGAA+GGG + chr1.2:33498669-33498688 MS.gene040652:CDS 35.0%
!! GAAGAGTAGGTATTTTGAAG+GGG + chr1.2:33498670-33498689 MS.gene040652:CDS 35.0%
!!! AGCCTTTTATTTTGGTCCAA+AGG + chr1.2:33499774-33499793 MS.gene040652:intron 35.0%
!!! GACGTCTTTAGGTTTTTTGT+TGG + chr1.2:33499198-33499217 MS.gene040652:intron 35.0%
AAAAACACCTGACAGTGTTC+AGG + chr1.2:33499484-33499503 MS.gene040652:intron 40.0%
AAACCTTGATCATCCGATTG+AGG - chr1.2:33498228-33498247 None:intergenic 40.0%
AACCTTGATCATCCGATTGA+GGG - chr1.2:33498227-33498246 None:intergenic 40.0%
ACAATCACAAAACCACCTGT+TGG + chr1.2:33499721-33499740 MS.gene040652:intron 40.0%
AGCAAAAGGATGTTCACCTT+TGG - chr1.2:33499793-33499812 None:intergenic 40.0%
AGTCACTGTAATCCTCCATA+TGG - chr1.2:33498923-33498942 None:intergenic 40.0%
CCAAAATAAAAGGCTACAGG+AGG - chr1.2:33499769-33499788 None:intergenic 40.0%
CTCATCCTCCTGCAAAAAAT+CGG - chr1.2:33498055-33498074 None:intergenic 40.0%
GGAACACAAAGGTCTAAGAA+GGG + chr1.2:33500159-33500178 MS.gene040652:CDS 40.0%
GGACCAAAATAAAAGGCTAC+AGG - chr1.2:33499772-33499791 None:intergenic 40.0%
GGTTTCATTATTGCGTCGTT+TGG + chr1.2:33498958-33498977 MS.gene040652:intron 40.0%
GTTACCTCCAAGAGAAAACA+TGG + chr1.2:33500138-33500157 MS.gene040652:CDS 40.0%
TCATTCTGACAAAGACCAAC+AGG - chr1.2:33499739-33499758 None:intergenic 40.0%
TCGAGGTTACCGAATTTGAT+GGG + chr1.2:33498521-33498540 MS.gene040652:intron 40.0%
TGGAACACAAAGGTCTAAGA+AGG + chr1.2:33500158-33500177 MS.gene040652:CDS 40.0%
TTCGAGGTTACCGAATTTGA+TGG + chr1.2:33498520-33498539 MS.gene040652:intron 40.0%
TTTCAGGCAGATGAGGAATA+GGG + chr1.2:33498592-33498611 MS.gene040652:intron 40.0%
! AGATTCTCAATCTCTGAGAG+AGG - chr1.2:33498117-33498136 None:intergenic 40.0%
! ATTCTCAATCTCTGAGAGAG+GGG - chr1.2:33498115-33498134 None:intergenic 40.0%
! CTTTCTTCTTTTCAACCTGC+TGG + chr1.2:33498479-33498498 MS.gene040652:intron 40.0%
! GATTCTCAATCTCTGAGAGA+GGG - chr1.2:33498116-33498135 None:intergenic 40.0%
! GTGACTTTGCACAGATGTTT+TGG + chr1.2:33498937-33498956 MS.gene040652:intron 40.0%
! TGTTCCATGTTTTCTCTTGG+AGG - chr1.2:33500145-33500164 None:intergenic 40.0%
! TTCAGCAGTGTTGAGAATAG+TGG + chr1.2:33499261-33499280 MS.gene040652:intron 40.0%
!! ATCTGATTTTCAGGCAGATG+AGG + chr1.2:33498585-33498604 MS.gene040652:intron 40.0%
!! CCTCCTGTAGCCTTTTATTT+TGG + chr1.2:33499766-33499785 MS.gene040652:intron 40.0%
!! TAAGAAGGGTGGCTCTAAAA+GGG + chr1.2:33500173-33500192 MS.gene040652:CDS 40.0%
!! TTTTCAGGCAGATGAGGAAT+AGG + chr1.2:33498591-33498610 MS.gene040652:intron 40.0%
!!! AGCTTTTTTGAAACGGGTTC+AGG - chr1.2:33498348-33498367 None:intergenic 40.0%
!!! TTTTTTGAAACGGGTTCAGG+AGG - chr1.2:33498345-33498364 None:intergenic 40.0%
AACACTGTTGTTAGCCACAG+TGG - chr1.2:33500121-33500140 None:intergenic 45.0%
ACACAAAGGTCTAAGAAGGG+TGG + chr1.2:33500162-33500181 MS.gene040652:CDS 45.0%
AGGAGTTACTGGATCAACTC+TGG - chr1.2:33498268-33498287 None:intergenic 45.0%
ATCCCTCAATCGGATGATCA+AGG + chr1.2:33498222-33498241 MS.gene040652:CDS 45.0%
ATGCTGAGAACCATGCTTTG+CGG + chr1.2:33498729-33498748 MS.gene040652:CDS 45.0%
CCATCAGACCCCATCAAATT+CGG - chr1.2:33498533-33498552 None:intergenic 45.0%
CGAGGTTACCGAATTTGATG+GGG + chr1.2:33498522-33498541 MS.gene040652:intron 45.0%
CTGCTGTGAAATCAAGAGAG+AGG + chr1.2:33498618-33498637 MS.gene040652:CDS 45.0%
CTTCTCATGTCTGATGCTGA+GGG + chr1.2:33498135-33498154 MS.gene040652:CDS 45.0%
GAGAATAGTGGTGTAGCAAG+TGG + chr1.2:33499273-33499292 MS.gene040652:intron 45.0%
GGCCAATATAGCTCATGTTG+CGG + chr1.2:33499334-33499353 MS.gene040652:intron 45.0%
TATATTGGCCGCTATAGCAG+CGG - chr1.2:33499324-33499343 None:intergenic 45.0%
TCATCAGAAGAAGGCTCTTG+AGG - chr1.2:33498288-33498307 None:intergenic 45.0%
TCTTCTCATGTCTGATGCTG+AGG + chr1.2:33498134-33498153 MS.gene040652:CDS 45.0%
TGAAGGGGAATGCAGAAGAT+TGG + chr1.2:33498685-33498704 MS.gene040652:CDS 45.0%
TGATCATCCGATTGAGGGAT+CGG - chr1.2:33498222-33498241 None:intergenic 45.0%
TGATCCAGTAACTCCTCAAG+AGG + chr1.2:33498272-33498291 MS.gene040652:CDS 45.0%
TGTTGTTAGCCACAGTGGAA+GGG - chr1.2:33500116-33500135 None:intergenic 45.0%
TTCTGACAAAGACCAACAGG+TGG - chr1.2:33499736-33499755 None:intergenic 45.0%
! TCAACTCTGGCTTCATCAGA+AGG - chr1.2:33498255-33498274 None:intergenic 45.0%
! TGGGGTCTGATGGATGATTA+TGG + chr1.2:33498540-33498559 MS.gene040652:intron 45.0%
!! CTAAGAAGGGTGGCTCTAAA+AGG + chr1.2:33500172-33500191 MS.gene040652:CDS 45.0%
!!! CGATTTTTTGCAGGAGGATG+AGG + chr1.2:33498053-33498072 MS.gene040652:CDS 45.0%
ACCCTGGCTTCATCAGAAGA+AGG - chr1.2:33498297-33498316 None:intergenic 50.0%
AGCCTTCTTCTGATGAAGCC+AGG + chr1.2:33498292-33498311 MS.gene040652:CDS 50.0%
AGGAGTTACTGGATCAACCC+TGG - chr1.2:33498313-33498332 None:intergenic 50.0%
ATGGTGATAGACCGGTGAGA+AGG - chr1.2:33499546-33499565 None:intergenic 50.0%
CATCTCATGCGGCACTTTAG+AGG - chr1.2:33499360-33499379 None:intergenic 50.0%
CCGAATTTGATGGGGTCTGA+TGG + chr1.2:33498530-33498549 MS.gene040652:intron 50.0%
CTGTTGTTAGCCACAGTGGA+AGG - chr1.2:33500117-33500136 None:intergenic 50.0%
GAAGGCTCTTGAGGAGTTAC+TGG - chr1.2:33498279-33498298 None:intergenic 50.0%
GAGTCTGCTGTGCTCTTGTT+GGG + chr1.2:33498800-33498819 MS.gene040652:CDS 50.0%
GATCATCCGATTGAGGGATC+GGG - chr1.2:33498221-33498240 None:intergenic 50.0%
GCAAACAAAGCCGCAAAGCA+TGG - chr1.2:33498742-33498761 None:intergenic 50.0%
GCCTTCTTCTGATGAAGCCA+GGG + chr1.2:33498293-33498312 MS.gene040652:CDS 50.0%
GGAACCTCTTGAGGAGTTAC+TGG - chr1.2:33498324-33498343 None:intergenic 50.0%
GGCCGCAACATGAGCTATAT+TGG - chr1.2:33499339-33499358 None:intergenic 50.0%
GGGAATTGCGTCGTCATCAT+CGG + chr1.2:33498155-33498174 MS.gene040652:CDS 50.0%
GTTGTTAGCCACAGTGGAAG+GGG - chr1.2:33500115-33500134 None:intergenic 50.0%
TGAGATGCCTGAACACTGTC+AGG - chr1.2:33499494-33499513 None:intergenic 50.0%
TGCTGTGCTCTTGTTGGGTA+AGG + chr1.2:33498805-33498824 MS.gene040652:intron 50.0%
! CTTTGTTTGCAATCGCGTGG+TGG + chr1.2:33498752-33498771 MS.gene040652:CDS 50.0%
! TCAATCTCTGAGAGAGGGGA+GGG - chr1.2:33498111-33498130 None:intergenic 50.0%
!!! AGTCGCCGATTTTTTGCAGG+AGG + chr1.2:33498047-33498066 MS.gene040652:CDS 50.0%
!!! CGAAGTCGCCGATTTTTTGC+AGG + chr1.2:33498044-33498063 MS.gene040652:CDS 50.0%
AGCAGACTCCTGCATGGTCA+TGG - chr1.2:33498789-33498808 None:intergenic 55.0%
GCAGACTCCTGCATGGTCAT+GGG - chr1.2:33498788-33498807 None:intergenic 55.0%
GGAGTCTGCTGTGCTCTTGT+TGG + chr1.2:33498799-33498818 MS.gene040652:CDS 55.0%
GGTTCAGGAGGAACCTCTTG+AGG - chr1.2:33498333-33498352 None:intergenic 55.0%
GGTTCCCTGCTATGGTTCCT+GGG + chr1.2:33500067-33500086 MS.gene040652:CDS 55.0%
GTGGAAGGGGTAGACTGAGT+TGG - chr1.2:33500102-33500121 None:intergenic 55.0%
GTGTCTGCGACGGAAACAGA+AGG - chr1.2:33498087-33498106 None:intergenic 55.0%
GTTGCAGAATCCCTGCTGCT+GGG + chr1.2:33500046-33500065 MS.gene040652:intron 55.0%
TAGTGCAGCCGCTGCTATAG+CGG + chr1.2:33499313-33499332 MS.gene040652:intron 55.0%
TCAGTCTACCCCTTCCACTG+TGG + chr1.2:33500104-33500123 MS.gene040652:CDS 55.0%
TGTTGCAGAATCCCTGCTGC+TGG + chr1.2:33500045-33500064 MS.gene040652:intron 55.0%
TTGCAATCGCGTGGTGGCTT+TGG + chr1.2:33498758-33498777 MS.gene040652:CDS 55.0%
! CTCAATCTCTGAGAGAGGGG+AGG - chr1.2:33498112-33498131 None:intergenic 55.0%
! GGGTAGACTGAGTTGGCACA+TGG - chr1.2:33500095-33500114 None:intergenic 55.0%
!! CGGCTTTGTTTGCAATCGCG+TGG + chr1.2:33498749-33498768 MS.gene040652:CDS 55.0%
ATAGCAGGGAACCCAGCAGC+AGG - chr1.2:33500060-33500079 None:intergenic 60.0%
CCTCGACCCGATCCCTCAAT+CGG + chr1.2:33498212-33498231 MS.gene040652:CDS 60.0%
GAGCACAGCAGACTCCTGCA+TGG - chr1.2:33498795-33498814 None:intergenic 60.0%
GGGTTCCCTGCTATGGTTCC+TGG + chr1.2:33500066-33500085 MS.gene040652:CDS 60.0%
GGTGCCCAGGAACCATAGCA+GGG - chr1.2:33500074-33500093 None:intergenic 60.0%
TAGCAGGGAACCCAGCAGCA+GGG - chr1.2:33500059-33500078 None:intergenic 60.0%
TGCTGCTGGGTTCCCTGCTA+TGG + chr1.2:33500059-33500078 MS.gene040652:CDS 60.0%
TGGTGCCCAGGAACCATAGC+AGG - chr1.2:33500075-33500094 None:intergenic 60.0%
TGGTGCTCCCATGACCATGC+AGG + chr1.2:33498778-33498797 MS.gene040652:CDS 60.0%
! TGAGTTGGCACATGGTGCCC+AGG - chr1.2:33500087-33500106 None:intergenic 60.0%
CCGATTGAGGGATCGGGTCG+AGG - chr1.2:33498215-33498234 None:intergenic 65.0%
!! AGGGGAGGGAGTGTCTGCGA+CGG - chr1.2:33498097-33498116 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 33497994 33500206 33497994 ID=MS.gene040652
chr1.2 mRNA 33497994 33500206 33497994 ID=MS.gene040652.t1;Parent=MS.gene040652
chr1.2 exon 33497994 33498373 33497994 ID=MS.gene040652.t1.exon1;Parent=MS.gene040652.t1
chr1.2 CDS 33497994 33498373 33497994 ID=cds.MS.gene040652.t1;Parent=MS.gene040652.t1
chr1.2 exon 33498598 33498821 33498598 ID=MS.gene040652.t1.exon2;Parent=MS.gene040652.t1
chr1.2 CDS 33498598 33498821 33498598 ID=cds.MS.gene040652.t1;Parent=MS.gene040652.t1
chr1.2 exon 33499922 33499967 33499922 ID=MS.gene040652.t1.exon3;Parent=MS.gene040652.t1
chr1.2 CDS 33499922 33499967 33499922 ID=cds.MS.gene040652.t1;Parent=MS.gene040652.t1
chr1.2 exon 33500053 33500206 33500053 ID=MS.gene040652.t1.exon4;Parent=MS.gene040652.t1
chr1.2 CDS 33500053 33500206 33500053 ID=cds.MS.gene040652.t1;Parent=MS.gene040652.t1
Gene Sequence

>MS.gene040652

ATGGACGATTTAGAACCCCAAATCGATTTCGAATCTCTCTTCGCAGAATACGAAGTCGCCGATTTTTTGCAGGAGGATGAGGATATCAATCCTTCTGTTTCCGTCGCAGACACTCCCTCCCCTCTCTCAGAGATTGAGAATCTTCTCATGTCTGATGCTGAGGGAATTGCGTCGTCATCATCGGATTCCGACTATCATAAGTTTCTCGAAGATATTCTCCTCGACCCGATCCCTCAATCGGATGATCAAGGTTTCGTTCCTTCTGATGAAGCCAGAGTTGATCCAGTAACTCCTCAAGAGCCTTCTTCTGATGAAGCCAGGGTTGATCCAGTAACTCCTCAAGAGGTTCCTCCTGAACCCGTTTCAAAAAAGCTAATTAGGTAAAACAAAATAATTTATAAGAATTGCCAATGTTGATCATATTCTGTAATTAAGACACATAAGTATTAATTAGTCTATAAGAATCATGATTTGTTTTTTTTTTTCTTTCTTCTTTTCAACCTGCTGGAGTAATTTGTAATTTGTATTCGAGGTTACCGAATTTGATGGGGTCTGATGGATGATTATGGTTCTAAAGAGAAGTTTTTTGGTATCTGATTTTCAGGCAGATGAGGAATAGGGATGCTGCTGTGAAATCAAGAGAGAGGAAGAAGATGTATGTGAAAAACCTTGAAACGAAGAGTAGGTATTTTGAAGGGGAATGCAGAAGATTGGAGCATTTGCTTCAATGCTGCTATGCTGAGAACCATGCTTTGCGGCTTTGTTTGCAATCGCGTGGTGGCTTTGGTGCTCCCATGACCATGCAGGAGTCTGCTGTGCTCTTGTTGGGTAAGGCTTGCAATTGCAAATCATATTTCATTTGCTTCTTTTCCTATTGTCGTTGCCATTTTATTTTTCGTATTTTAGTTTAACTGCTAAAAGTATAACCATATGGAGGATTACAGTGACTTTGCACAGATGTTTTGGTTTCATTATTGCGTCGTTTGGCCTAAAATAATTTGCCTACTTTCTTGAATGAATGGATCTTGTGCTAATGCTCTATAGTTTCATCTGTCGGCTTTATCATAGTTACCCAAGTTTTAACCTTAATCTCTAATGATTATCGTTACAAATATATTTATGCTAACTTGTTAGTTGATATGAATAATATGAATAATATGCTTCTTTGTCAGTAGAATAACTGTCATGTAGATCAGATGTTAAAGACGTCTTTAGGTTTTTTGTTGGGTTTTGCAGAACACAAAGAACTAGGTGAATTTATTTTATGTTCAGCAGTGTTGAGAATAGTGGTGTAGCAAGTGGCTGCTACAAACAAGCAGTAGTGCAGCCGCTGCTATAGCGGCCAATATAGCTCATGTTGCGGCCTCTAAAGTGCCGCATGAGATGAGATCTGAAATTCAAATGAAAAAAATTTTGACAATAAAAAATCCCATGAAACATAAATCATTAGATCTTTTTAGTTGGAGTATCTTAGGAATTTTTCAGTTTTGAAAAACACCTGACAGTGTTCAGGCATCTCATTCTGTCTCATTCAAGCTATAATATTTCACCTTCTCACCGGTCTATCACCATAGTTATTGAGCTTTATGTATGTACTGGTCATCCCACTATAGTGTTTTTGAGGTCAGCAACTATGCTATGTTATCTGGGATTGATACATTACCACTGTTGTTTAGGTTTTTGCATCTAAATGGAAAAAATTTGTGTGGGTTGTATGGATAATAATAACAATCACAAAACCACCTGTTGGTCTTTGTCAGAATGAAAGTCTACCTCCTGTAGCCTTTTATTTTGGTCCAAAGGTGAACATCCTTTTGCTGTAATCCAGATTTGTGTGCTTATGCAAAATACATAAAACATATACAACATGGAAATTCCAAAACTCTCAGAGAAAAAGTAGATTCCCTTTATGCATGTTAAATGAATAGAAAGTGATTTTGATATGTCAATTCCAATGTTGCTATCATTCAAAGAGTGAGTTTTATGAAGCTGCTCTAATGGAGTTCATGTTAATTAGATTATCATTTATTTACAAATATTAATCATATTCATGTTGCAGAATCCCTGCTGCTGGGTTCCCTGCTATGGTTCCTGGGCACCATGTGCCAACTCAGTCTACCCCTTCCACTGTGGCTAACAACAGTGTTACCTCCAAGAGAAAACATGGAACACAAAGGTCTAAGAAGGGTGGCTCTAAAAGGGCCAAACAGTAA
Protein sequence

>MS.gene040652.t1

MDDLEPQIDFESLFAEYEVADFLQEDEDINPSVSVADTPSPLSEIENLLMSDAEGIASSSSDSDYHKFLEDILLDPIPQSDDQGFVPSDEARVDPVTPQEPSSDEARVDPVTPQEVPPEPVSKKLIRQMRNRDAAVKSRERKKMYVKNLETKSRYFEGECRRLEHLLQCCYAENHALRLCLQSRGGFGAPMTMQESAVLLLESDFDMSIPMLLSFKEIPAAGFPAMVPGHHVPTQSTPSTVANNSVTSKRKHGTQRSKKGGSKRAKQ