AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040735.t1 MTR_1g038370 87.759 580 49 3 1 579 1 559 0.0 1043
MS.gene040735.t1 MTR_1g038400 76.748 572 105 3 2 571 22 567 0.0 874
MS.gene040735.t1 MTR_5g029370 52.209 498 232 2 2 494 83 579 0.0 549
MS.gene040735.t1 MTR_5g029370 52.209 498 232 2 2 494 83 579 0.0 549
MS.gene040735.t1 MTR_7g407000 81.250 144 9 2 181 323 28 154 4.34e-77 241
MS.gene040735.t1 MTR_3g075210 38.567 363 179 8 9 346 19 362 1.52e-66 236
MS.gene040735.t1 MTR_8g089260 36.504 389 170 9 4 325 29 407 8.92e-58 209
MS.gene040735.t1 MTR_1g078070 30.540 537 280 16 6 463 148 670 3.31e-54 199
MS.gene040735.t1 MTR_1g078070 29.981 537 283 16 6 463 148 670 3.55e-54 199
MS.gene040735.t1 MTR_5g010300 35.864 382 200 11 109 464 323 685 9.69e-54 198
MS.gene040735.t1 MTR_5g010300 35.864 382 200 11 109 464 323 685 1.04e-53 198
MS.gene040735.t1 MTR_5g010300 35.864 382 200 11 109 464 323 685 1.22e-53 197
MS.gene040735.t1 MTR_1g094740 45.745 188 98 1 152 339 291 474 6.01e-53 196
MS.gene040735.t1 MTR_5g010300 42.264 265 124 6 109 354 323 577 1.04e-52 195
MS.gene040735.t1 MTR_2g047130 33.007 409 195 11 4 344 48 445 1.40e-51 191
MS.gene040735.t1 MTR_2g047130 33.007 409 195 11 4 344 45 442 1.66e-51 191
MS.gene040735.t1 MTR_7g097070 32.508 323 179 11 156 456 338 643 8.97e-30 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040735.t1 AT5G46910 54.506 466 211 1 7 472 102 566 0.0 539
MS.gene040735.t1 AT5G46910 54.506 466 211 1 7 472 102 566 0.0 538
MS.gene040735.t1 AT2G34880 31.343 536 281 16 7 471 60 579 1.03e-57 207
MS.gene040735.t1 AT1G63490 42.143 280 135 7 79 340 37 307 2.38e-57 208
MS.gene040735.t1 AT3G48430 34.783 368 198 6 9 339 21 383 2.96e-57 208
MS.gene040735.t1 AT1G30810 29.870 539 290 13 4 467 55 580 3.25e-57 206
MS.gene040735.t1 AT1G30810 29.870 539 290 13 4 467 55 580 3.25e-57 206
MS.gene040735.t1 AT1G30810 29.870 539 290 13 4 467 55 580 3.25e-57 206
MS.gene040735.t1 AT1G63490 36.061 391 180 12 79 431 290 648 4.65e-57 207
MS.gene040735.t1 AT1G63490 42.143 280 135 7 79 340 290 560 4.79e-57 207
MS.gene040735.t1 AT4G20400 27.642 615 326 20 4 529 52 636 1.16e-54 200
MS.gene040735.t1 AT1G08620 32.195 410 198 8 6 343 158 559 5.95e-54 198
MS.gene040735.t1 AT1G08620 32.195 410 198 8 6 343 144 545 7.17e-54 198
MS.gene040735.t1 AT1G08620 32.195 410 198 8 6 343 144 545 7.17e-54 198
MS.gene040735.t1 AT1G08620 32.195 410 198 8 6 343 144 545 7.17e-54 198
MS.gene040735.t1 AT5G04240 46.277 188 97 1 152 339 259 442 5.29e-52 192
MS.gene040735.t1 AT4G20400 28.239 602 315 20 16 529 7 579 1.10e-51 191
MS.gene040735.t1 AT2G38950 24.723 631 368 20 2 550 102 707 7.94e-35 140
MS.gene040735.t1 AT1G63490 52.874 87 41 0 254 340 15 101 3.99e-24 108

Find 86 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATTGTACAGCCTCTT+AGG 0.195974 1.2:+35149611 MS.gene040735:CDS
TGTCTTAATGGAAGAGAAAA+AGG 0.204332 1.2:+35149576 MS.gene040735:CDS
GATGCATTCATTTATGTTAT+AGG 0.215836 1.2:-35151861 None:intergenic
TAGGAGAATATATTTGTTAA+AGG 0.243804 1.2:-35151842 None:intergenic
AATCCAGCATGATATGAATT+TGG 0.254423 1.2:-35150708 None:intergenic
TTCTTTACCACGCAATCTAT+TGG 0.264442 1.2:-35149820 None:intergenic
TCCTTCAATATCTTCCCTTT+TGG 0.303945 1.2:+35152297 MS.gene040735:CDS
ACTGAAAACAGAATAATAAT+TGG 0.315714 1.2:+35152059 MS.gene040735:intron
AGTTCCTCATATGGAATTAT+AGG 0.316333 1.2:-35151320 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1.2:+35149897 MS.gene040735:CDS
ATAAAGGACTCGTTGTCTTT+TGG 0.343992 1.2:-35152236 None:intergenic
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1.2:-35151329 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1.2:-35151605 None:intergenic
AGCTCTTCAAATACATCATT+AGG 0.370354 1.2:+35151509 MS.gene040735:CDS
CAAAATCAAACTTGAAGATA+TGG 0.374066 1.2:+35151394 MS.gene040735:CDS
TGACATGCAGCCTCTGCAAT+AGG 0.375602 1.2:+35151537 MS.gene040735:CDS
AACAAGCAATTTCAATTTGA+AGG 0.380018 1.2:+35149971 MS.gene040735:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1.2:+35151246 MS.gene040735:CDS
CTCCATTGCGTTTAGTTGAC+AGG 0.390855 1.2:+35150178 MS.gene040735:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1.2:-35149557 None:intergenic
GGAAGATATTGAAGGAACTT+TGG 0.410410 1.2:-35152290 None:intergenic
CTGATCCTATGCTTTACATT+GGG 0.424948 1.2:+35150302 MS.gene040735:CDS
TGCTGCCAAAAGTCTTCAAC+AGG 0.428587 1.2:+35151796 MS.gene040735:CDS
CACAATTGTACAGCCTCTTA+GGG 0.435355 1.2:+35149612 MS.gene040735:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1.2:-35149954 None:intergenic
TAAGACATCGCTATCGCGGT+TGG 0.443672 1.2:+35151466 MS.gene040735:CDS
TTGGTGATTGGTTTCCACTC+GGG 0.449709 1.2:+35151258 MS.gene040735:CDS
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1.2:+35151239 MS.gene040735:CDS
TTTGATTCGAAAACCCTAAG+AGG 0.457545 1.2:-35149625 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1.2:+35151316 MS.gene040735:CDS
TAACTTGAGAAAACATGAAT+CGG 0.462602 1.2:+35152258 MS.gene040735:CDS
AAGACATCGCTATCGCGGTT+GGG 0.473169 1.2:+35151467 MS.gene040735:CDS
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1.2:-35149052 None:intergenic
ATACCAAATTTAGAAGCCTC+GGG 0.480285 1.2:-35149103 None:intergenic
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1.2:+35150666 MS.gene040735:CDS
TTAAAAGAACATGGAGAGAA+TGG 0.487266 1.2:+35150570 MS.gene040735:CDS
ACATAATGTGCCGGTTTACA+AGG 0.487938 1.2:+35150650 MS.gene040735:CDS
TTGAAGATCACTATTTGTAC+AGG 0.488775 1.2:+35150353 MS.gene040735:CDS
ACAAGGTCTTACCATGGAAA+AGG 0.491516 1.2:-35151613 None:intergenic
ATTTGAAGCTCTTGCAAACA+AGG 0.496352 1.2:+35149791 MS.gene040735:CDS
TTTCCAAATTCATATCATGC+TGG 0.496493 1.2:+35150705 MS.gene040735:CDS
TGCCACAAAATTGTTTATGC+GGG 0.497644 1.2:+35151726 MS.gene040735:CDS
GACATGCAGCCTCTGCAATA+GGG 0.498995 1.2:+35151538 MS.gene040735:CDS
CATACCAAATTTAGAAGCCT+CGG 0.500972 1.2:-35149104 None:intergenic
ATCATCAGGATCACTTGAAA+AGG 0.501772 1.2:-35149940 None:intergenic
GGTGCAAATAAAACTTGGTA+TGG 0.503293 1.2:+35150480 MS.gene040735:CDS
ATTGGTGATTGGTTTCCACT+CGG 0.503752 1.2:+35151257 MS.gene040735:CDS
GAAATAGTACATGGAGAGAA+AGG 0.511259 1.2:+35149882 MS.gene040735:CDS
TCCATTAAGACAAAAGAAGC+AGG 0.512314 1.2:-35149565 None:intergenic
ACTGATCCTATGCTTTACAT+TGG 0.515442 1.2:+35150301 MS.gene040735:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1.2:+35149948 MS.gene040735:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1.2:+35150326 MS.gene040735:CDS
TGGTGATTGGTTTCCACTCG+GGG 0.519820 1.2:+35151259 MS.gene040735:CDS
GAGAACATACTGTATTTAAG+AGG 0.523058 1.2:+35151750 MS.gene040735:CDS
AATTGAGAGGATGCAGAACT+AGG 0.531709 1.2:-35150507 None:intergenic
ACTCAGGTGCAAATAAAACT+TGG 0.537060 1.2:+35150475 MS.gene040735:CDS
TTATGTTCTAACAAGACATC+AGG 0.538881 1.2:-35150633 None:intergenic
CTTGTTAGAACATAATGTGC+CGG 0.547471 1.2:+35150641 MS.gene040735:CDS
AATGGAGACTGTGGCAGCCG+CGG 0.554838 1.2:-35150162 None:intergenic
GCAGGATTAGAGGCAGCGAT+TGG 0.560174 1.2:-35149547 None:intergenic
TTTATAAGACATCGCTATCG+CGG 0.560344 1.2:+35151462 MS.gene040735:CDS
AAGGTGACGACAAACTCTCC+TGG 0.561754 1.2:-35150684 None:intergenic
CAAGACATCAGGAGAGAACA+TGG 0.563327 1.2:-35150622 None:intergenic
TGTTCTGATGGATAATACGT+TGG 0.567602 1.2:-35149040 None:intergenic
GCCAAAAGGGAAGATATTGA+AGG 0.571210 1.2:-35152298 None:intergenic
TTCCTGTCAACTAAACGCAA+TGG 0.577093 1.2:-35150180 None:intergenic
AGAAGAACTGTTAACTCACT+AGG 0.579133 1.2:+35152100 MS.gene040735:CDS
GTGCCACAAAATTGTTTATG+CGG 0.580719 1.2:+35151725 MS.gene040735:CDS
ATTTAAGAGGAATGACATGT+TGG 0.584034 1.2:+35151763 MS.gene040735:CDS
TTCTGGCACGAAATAGTACA+TGG 0.584729 1.2:+35149873 MS.gene040735:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1.2:+35150717 MS.gene040735:CDS
ATTTGTTAAAGGTAACGCGT+TGG 0.609530 1.2:-35151831 None:intergenic
TTGCTAGCTGCAGCCCCGAG+TGG 0.613167 1.2:-35151272 None:intergenic
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1.2:-35150307 None:intergenic
TACAACTCCTCAAAAGCTAG+AGG 0.624733 1.2:+35151368 MS.gene040735:CDS
AGCATAAATTACCATCACTC+AGG 0.628951 1.2:+35150459 MS.gene040735:intron
ACAGAATAATAATTGGCATG+AGG 0.629929 1.2:+35152066 MS.gene040735:intron
AGCATGATATGAATTTGGAA+AGG 0.636839 1.2:-35150703 None:intergenic
AACAAAGTCTTAAAAGAACA+TGG 0.646276 1.2:+35150561 MS.gene040735:CDS
CATAACAATCCCTATTGCAG+AGG 0.648673 1.2:-35151547 None:intergenic
TGAATGCATCAAAGAACACA+AGG 0.658928 1.2:+35151874 MS.gene040735:CDS
ACCAAATTTAGAAGCCTCGG+GGG 0.680089 1.2:-35149101 None:intergenic
TACCAAATTTAGAAGCCTCG+GGG 0.686654 1.2:-35149102 None:intergenic
ACTAAACGCAATGGAGACTG+TGG 0.698121 1.2:-35150171 None:intergenic
TAATGTGCCGGTTTACAAGG+CGG 0.701604 1.2:+35150653 MS.gene040735:CDS
TGAGTATGGAGTCAATGTTG+AGG 0.703101 1.2:+35149911 MS.gene040735:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTATTCAAAATTTCA+GGG + chr1.2:35150274-35150293 MS.gene040735:intron 10.0%
!!! TTAATTTTATTCAAAATTTC+AGG + chr1.2:35150273-35150292 MS.gene040735:intron 10.0%
!! ATATTTGATCTTAATATTTC+AGG + chr1.2:35149505-35149524 MS.gene040735:intron 15.0%
!!! AACAACTTTTAATTCATATT+AGG - chr1.2:35150234-35150253 None:intergenic 15.0%
!! ACTATTATAATGAAGTTTAG+AGG - chr1.2:35151943-35151962 None:intergenic 20.0%
!! ACTGAAAACAGAATAATAAT+TGG + chr1.2:35152059-35152078 MS.gene040735:intron 20.0%
!! AGAACGATATAATCTAATAT+AGG + chr1.2:35149414-35149433 MS.gene040735:intron 20.0%
!! TGATGAAAAATTAACTAACT+TGG + chr1.2:35149728-35149747 MS.gene040735:intron 20.0%
!!! AATCTGTTTATTTTTGTACA+AGG + chr1.2:35150995-35151014 MS.gene040735:intron 20.0%
!!! ACATAAGTATTTAAAATAGC+AGG - chr1.2:35151114-35151133 None:intergenic 20.0%
!!! ATAACTTTTTCTAAGAGAAA+AGG + chr1.2:35149661-35149680 MS.gene040735:CDS 20.0%
!!! ATCTGATTGATTTTAACTTT+TGG + chr1.2:35152029-35152048 MS.gene040735:intron 20.0%
!!! CTTATATTTTACGAATAAGT+AGG + chr1.2:35150823-35150842 MS.gene040735:intron 20.0%
!!! TAACTTTTTCTAAGAGAAAA+GGG + chr1.2:35149662-35149681 MS.gene040735:CDS 20.0%
!!! TAGGAGAATATATTTGTTAA+AGG - chr1.2:35151845-35151864 None:intergenic 20.0%
!!! TTTTTTTCCATAATAATACC+TGG + chr1.2:35151075-35151094 MS.gene040735:intron 20.0%
! AACAAAGTCTTAAAAGAACA+TGG + chr1.2:35150561-35150580 MS.gene040735:CDS 25.0%
! AACAAGCAATTTCAATTTGA+AGG + chr1.2:35149971-35149990 MS.gene040735:CDS 25.0%
! AATACTCAAAGAGAATAACA+AGG - chr1.2:35151633-35151652 None:intergenic 25.0%
! AGTAGAATTCTTACTATACT+TGG - chr1.2:35149228-35149247 None:intergenic 25.0%
! ATAAAAAAACCCTAGAAACA+TGG - chr1.2:35149164-35149183 None:intergenic 25.0%
! ATGGATATACTTGCTTATTA+TGG + chr1.2:35151687-35151706 MS.gene040735:intron 25.0%
! CAAAATCAAACTTGAAGATA+TGG + chr1.2:35151394-35151413 MS.gene040735:CDS 25.0%
! CTTTCTATTATTCAAGCTTA+GGG - chr1.2:35150902-35150921 None:intergenic 25.0%
! GATGCATTCATTTATGTTAT+AGG - chr1.2:35151864-35151883 None:intergenic 25.0%
! TAACTTGAGAAAACATGAAT+CGG + chr1.2:35152258-35152277 MS.gene040735:CDS 25.0%
!! AGATTTTACTAAAATCACAG+TGG + chr1.2:35149375-35149394 MS.gene040735:intron 25.0%
!! AGGTTGATTGAATAATAGAA+AGG + chr1.2:35150847-35150866 MS.gene040735:intron 25.0%
!! ATCATTTTCTTGTTGAGTTT+AGG + chr1.2:35151203-35151222 MS.gene040735:intron 25.0%
!! ATTTTCTGCAGATAAACTAA+AGG - chr1.2:35149079-35149098 None:intergenic 25.0%
!! CATGTTTTCTCAAGTTATAA+AGG - chr1.2:35152255-35152274 None:intergenic 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG - chr1.2:35150602-35150621 None:intergenic 25.0%
AAAGAAAGTTGAGGGATAAA+AGG - chr1.2:35149262-35149281 None:intergenic 30.0%
AATCCAGCATGATATGAATT+TGG - chr1.2:35150711-35150730 None:intergenic 30.0%
ACAGAATAATAATTGGCATG+AGG + chr1.2:35152066-35152085 MS.gene040735:intron 30.0%
AGCATGATATGAATTTGGAA+AGG - chr1.2:35150706-35150725 None:intergenic 30.0%
AGCTCTTCAAATACATCATT+AGG + chr1.2:35151509-35151528 MS.gene040735:CDS 30.0%
AGTTCCTCATATGGAATTAT+AGG - chr1.2:35151323-35151342 None:intergenic 30.0%
ATTTAAGAGGAATGACATGT+TGG + chr1.2:35151763-35151782 MS.gene040735:CDS 30.0%
CCTAAGCTTGAATAATAGAA+AGG + chr1.2:35150900-35150919 MS.gene040735:intron 30.0%
CCTTTCTATTATTCAAGCTT+AGG - chr1.2:35150903-35150922 None:intergenic 30.0%
GAGAACATACTGTATTTAAG+AGG + chr1.2:35151750-35151769 MS.gene040735:CDS 30.0%
TAACACATTGCTTTATCTTC+AGG + chr1.2:35151990-35152009 MS.gene040735:intron 30.0%
TACAAAAATTGCTTCCTTAC+TGG - chr1.2:35150210-35150229 None:intergenic 30.0%
TACGTGCATGTTTAGATTAA+CGG + chr1.2:35149309-35149328 MS.gene040735:intron 30.0%
TGTCTTAATGGAAGAGAAAA+AGG + chr1.2:35149576-35149595 MS.gene040735:CDS 30.0%
TTAAAAGAACATGGAGAGAA+TGG + chr1.2:35150570-35150589 MS.gene040735:CDS 30.0%
TTACAAAGAAGTTCCTCATA+TGG - chr1.2:35151332-35151351 None:intergenic 30.0%
TTATGTTCTAACAAGACATC+AGG - chr1.2:35150636-35150655 None:intergenic 30.0%
TTGAAGATCACTATTTGTAC+AGG + chr1.2:35150353-35150372 MS.gene040735:CDS 30.0%
TTTCCAAATTCATATCATGC+TGG + chr1.2:35150705-35150724 MS.gene040735:CDS 30.0%
! TATTTTACGAATAAGTAGGC+AGG + chr1.2:35150827-35150846 MS.gene040735:intron 30.0%
! TGAATAATAGAAAGGTACGT+AGG + chr1.2:35150855-35150874 MS.gene040735:intron 30.0%
! TTTTCCATAATAATACCTGG+CGG + chr1.2:35151078-35151097 MS.gene040735:intron 30.0%
! TTTTTCCTGTTGAAGACTTT+TGG - chr1.2:35151804-35151823 None:intergenic 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + chr1.2:35151246-35151265 MS.gene040735:CDS 30.0%
!! TTTTCATCACCATGTTTCTA+GGG + chr1.2:35149152-35149171 MS.gene040735:intron 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG + chr1.2:35151215-35151234 MS.gene040735:intron 30.0%
!!! TTTGATTTTAGAATTTGCCG+CGG + chr1.2:35150145-35150164 MS.gene040735:intron 30.0%
AATTCCGCCAGGTATTATTA+TGG - chr1.2:35151085-35151104 None:intergenic 35.0%
ACTCAGGTGCAAATAAAACT+TGG + chr1.2:35150475-35150494 MS.gene040735:CDS 35.0%
ACTGATCCTATGCTTTACAT+TGG + chr1.2:35150301-35150320 MS.gene040735:CDS 35.0%
AGAAGAACTGTTAACTCACT+AGG + chr1.2:35152100-35152119 MS.gene040735:CDS 35.0%
AGAATAACAAGGTCTTACCA+TGG - chr1.2:35151622-35151641 None:intergenic 35.0%
AGCATAAATTACCATCACTC+AGG + chr1.2:35150459-35150478 MS.gene040735:intron 35.0%
ATACCAAATTTAGAAGCCTC+GGG - chr1.2:35149106-35149125 None:intergenic 35.0%
ATCATCAGGATCACTTGAAA+AGG - chr1.2:35149943-35149962 None:intergenic 35.0%
ATGGATGCAAAAGAAAGTTG+AGG - chr1.2:35149271-35149290 None:intergenic 35.0%
CATACCAAATTTAGAAGCCT+CGG - chr1.2:35149107-35149126 None:intergenic 35.0%
CGAGATTTGATTATGGAGTT+CGG - chr1.2:35150963-35150982 None:intergenic 35.0%
CTGATCCTATGCTTTACATT+GGG + chr1.2:35150302-35150321 MS.gene040735:CDS 35.0%
CTTGTTAGAACATAATGTGC+CGG + chr1.2:35150641-35150660 MS.gene040735:CDS 35.0%
GAAATAGTACATGGAGAGAA+AGG + chr1.2:35149882-35149901 MS.gene040735:CDS 35.0%
GCTACCTATAATTCCATATG+AGG + chr1.2:35151316-35151335 MS.gene040735:CDS 35.0%
GGAAGATATTGAAGGAACTT+TGG - chr1.2:35152293-35152312 None:intergenic 35.0%
GGTGCAAATAAAACTTGGTA+TGG + chr1.2:35150480-35150499 MS.gene040735:CDS 35.0%
GTGCCACAAAATTGTTTATG+CGG + chr1.2:35151725-35151744 MS.gene040735:CDS 35.0%
TATCATGCTGGATTTAGTCA+TGG + chr1.2:35150717-35150736 MS.gene040735:CDS 35.0%
TCCATTAAGACAAAAGAAGC+AGG - chr1.2:35149568-35149587 None:intergenic 35.0%
TGAATGCATCAAAGAACACA+AGG + chr1.2:35151874-35151893 MS.gene040735:CDS 35.0%
TGCCACAAAATTGTTTATGC+GGG + chr1.2:35151726-35151745 MS.gene040735:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + chr1.2:35150326-35150345 MS.gene040735:CDS 35.0%
TGGATGCAAAAGAAAGTTGA+GGG - chr1.2:35149270-35149289 None:intergenic 35.0%
TTACCATGGAAAAGGCAAAT+TGG - chr1.2:35151608-35151627 None:intergenic 35.0%
TTCTTTACCACGCAATCTAT+TGG - chr1.2:35149823-35149842 None:intergenic 35.0%
TTGACAGGAAAATTCCAGTA+AGG + chr1.2:35150193-35150212 MS.gene040735:intron 35.0%
TTTATAAGACATCGCTATCG+CGG + chr1.2:35151462-35151481 MS.gene040735:CDS 35.0%
! AGAAAGGTACGTAGGTTTAA+AGG + chr1.2:35150863-35150882 MS.gene040735:intron 35.0%
! ATAAAGGACTCGTTGTCTTT+TGG - chr1.2:35152239-35152258 None:intergenic 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG + chr1.2:35149791-35149810 MS.gene040735:CDS 35.0%
! ATTTGTTAAAGGTAACGCGT+TGG - chr1.2:35151834-35151853 None:intergenic 35.0%
! CTTTTCATCACCATGTTTCT+AGG + chr1.2:35149151-35149170 MS.gene040735:intron 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + chr1.2:35151239-35151258 MS.gene040735:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - chr1.2:35150523-35150542 None:intergenic 35.0%
! GCAACAGATTCTGCATTTTT+AGG + chr1.2:35149449-35149468 MS.gene040735:intron 35.0%
! GGTTTTCGAATCAAATGAGA+TGG + chr1.2:35149633-35149652 MS.gene040735:CDS 35.0%
! TAAGCTGCTTGTGTTATTGA+TGG + chr1.2:35151668-35151687 MS.gene040735:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+TGG + chr1.2:35149564-35149583 MS.gene040735:CDS 35.0%
! TCCTTCAATATCTTCCCTTT+TGG + chr1.2:35152297-35152316 MS.gene040735:CDS 35.0%
! TGTTCTGATGGATAATACGT+TGG - chr1.2:35149043-35149062 None:intergenic 35.0%
! TTTGATTCGAAAACCCTAAG+AGG - chr1.2:35149628-35149647 None:intergenic 35.0%
!! ATTTTTCAGATGTCGCCAAA+AGG - chr1.2:35152315-35152334 None:intergenic 35.0%
!! TGGTGTTATTACGTTTCTGA+TGG + chr1.2:35150794-35150813 MS.gene040735:intron 35.0%
!! TTTTATTTGCACCTGAGTGA+TGG - chr1.2:35150473-35150492 None:intergenic 35.0%
!! TTTTTCAGATGTCGCCAAAA+GGG - chr1.2:35152314-35152333 None:intergenic 35.0%
AATTGAGAGGATGCAGAACT+AGG - chr1.2:35150510-35150529 None:intergenic 40.0%
ACAAGGTCTTACCATGGAAA+AGG - chr1.2:35151616-35151635 None:intergenic 40.0%
ACACAATTGTACAGCCTCTT+AGG + chr1.2:35149611-35149630 MS.gene040735:CDS 40.0%
AGATCTCTCTCTACATGTGT+TGG + chr1.2:35150774-35150793 MS.gene040735:intron 40.0%
AGCATCCCAATGTAAAGCAT+AGG - chr1.2:35150310-35150329 None:intergenic 40.0%
AGTGATCCTGATGATAAGCT+TGG + chr1.2:35149948-35149967 MS.gene040735:CDS 40.0%
CACAATTGTACAGCCTCTTA+GGG + chr1.2:35149612-35149631 MS.gene040735:CDS 40.0%
CATAACAATCCCTATTGCAG+AGG - chr1.2:35151550-35151569 None:intergenic 40.0%
CTTGTTCCAAGCTTATCATC+AGG - chr1.2:35149957-35149976 None:intergenic 40.0%
GAACACGCGAGATTTGATTA+TGG - chr1.2:35150970-35150989 None:intergenic 40.0%
GACAAAAGAAGCAGGATTAG+AGG - chr1.2:35149560-35149579 None:intergenic 40.0%
GAGAAAGGAACAGTTGAGTA+TGG + chr1.2:35149897-35149916 MS.gene040735:CDS 40.0%
GCCAAAAGGGAAGATATTGA+AGG - chr1.2:35152301-35152320 None:intergenic 40.0%
TACAACTCCTCAAAAGCTAG+AGG + chr1.2:35151368-35151387 MS.gene040735:CDS 40.0%
TACCAAATTTAGAAGCCTCG+GGG - chr1.2:35149105-35149124 None:intergenic 40.0%
TGAGTATGGAGTCAATGTTG+AGG + chr1.2:35149911-35149930 MS.gene040735:CDS 40.0%
TGCTCAAACTCTTGTTCTGA+TGG - chr1.2:35149055-35149074 None:intergenic 40.0%
TTCCTGTCAACTAAACGCAA+TGG - chr1.2:35150183-35150202 None:intergenic 40.0%
TTCTGGCACGAAATAGTACA+TGG + chr1.2:35149873-35149892 MS.gene040735:CDS 40.0%
! CTCCCGCATAAACAATTTTG+TGG - chr1.2:35151731-35151750 None:intergenic 40.0%
! CTTTTGGTACTCATCACTGA+TGG - chr1.2:35152223-35152242 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG + chr1.2:35151602-35151621 MS.gene040735:CDS 40.0%
!! ACATAATGTGCCGGTTTACA+AGG + chr1.2:35150650-35150669 MS.gene040735:CDS 40.0%
!! ATTGGTGATTGGTTTCCACT+CGG + chr1.2:35151257-35151276 MS.gene040735:CDS 40.0%
!! CAGATATCGAAAAAGCGTTC+TGG + chr1.2:35149856-35149875 MS.gene040735:CDS 40.0%
!!! TTTGTCACCTCTAGCTTTTG+AGG - chr1.2:35151378-35151397 None:intergenic 40.0%
AAACTCGAGCACTTCAGAGA+TGG - chr1.2:35149290-35149309 None:intergenic 45.0%
ACCAAATTTAGAAGCCTCGG+GGG - chr1.2:35149104-35149123 None:intergenic 45.0%
ACTAAACGCAATGGAGACTG+TGG - chr1.2:35150174-35150193 None:intergenic 45.0%
CAAGACATCAGGAGAGAACA+TGG - chr1.2:35150625-35150644 None:intergenic 45.0%
CTCCATTGCGTTTAGTTGAC+AGG + chr1.2:35150178-35150197 MS.gene040735:CDS 45.0%
TGCTGCCAAAAGTCTTCAAC+AGG + chr1.2:35151796-35151815 MS.gene040735:CDS 45.0%
! GCGTTTTCCAATAGATTGCG+TGG + chr1.2:35149813-35149832 MS.gene040735:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+CGG - chr1.2:35150663-35150682 None:intergenic 45.0%
!! TAATGTGCCGGTTTACAAGG+CGG + chr1.2:35150653-35150672 MS.gene040735:CDS 45.0%
!! TTGGTGATTGGTTTCCACTC+GGG + chr1.2:35151258-35151277 MS.gene040735:CDS 45.0%
AAGACATCGCTATCGCGGTT+GGG + chr1.2:35151467-35151486 MS.gene040735:CDS 50.0%
AAGGTGACGACAAACTCTCC+TGG - chr1.2:35150687-35150706 None:intergenic 50.0%
GACATGCAGCCTCTGCAATA+GGG + chr1.2:35151538-35151557 MS.gene040735:CDS 50.0%
TAAGACATCGCTATCGCGGT+TGG + chr1.2:35151466-35151485 MS.gene040735:CDS 50.0%
TGACATGCAGCCTCTGCAAT+AGG + chr1.2:35151537-35151556 MS.gene040735:CDS 50.0%
TGCAGCAGCAGAGGAACAAT+AGG + chr1.2:35150937-35150956 MS.gene040735:intron 50.0%
!! TGGTGATTGGTTTCCACTCG+GGG + chr1.2:35151259-35151278 MS.gene040735:CDS 50.0%
GCAGGAAGTGAAATTCCGCC+AGG - chr1.2:35151096-35151115 None:intergenic 55.0%
GCAGGATTAGAGGCAGCGAT+TGG - chr1.2:35149550-35149569 None:intergenic 55.0%
GCCCCCGAGGCTTCTAAATT+TGG + chr1.2:35149100-35149119 MS.gene040735:CDS 55.0%
GTTCCTCTGCTGCTGCATAG+TGG - chr1.2:35150934-35150953 None:intergenic 55.0%
TACAAGGCGGTGCAAAAGCC+AGG + chr1.2:35150666-35150685 MS.gene040735:CDS 55.0%
TCTGCAGAAAATTGCCCCCG+AGG + chr1.2:35149087-35149106 MS.gene040735:CDS 55.0%
AATGGAGACTGTGGCAGCCG+CGG - chr1.2:35150165-35150184 None:intergenic 60.0%
CCTCTGCTGCTGCATAGTGG+CGG - chr1.2:35150931-35150950 None:intergenic 60.0%
CCGCCACTATGCAGCAGCAG+AGG + chr1.2:35150928-35150947 MS.gene040735:intron 65.0%
TTGCTAGCTGCAGCCCCGAG+TGG - chr1.2:35151275-35151294 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 35149016 35152342 35149016 ID=MS.gene040735
chr1.2 mRNA 35149016 35152342 35149016 ID=MS.gene040735.t1;Parent=MS.gene040735
chr1.2 exon 35149016 35149121 35149016 ID=MS.gene040735.t1.exon1;Parent=MS.gene040735.t1
chr1.2 CDS 35149016 35149121 35149016 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35149527 35149683 35149527 ID=MS.gene040735.t1.exon2;Parent=MS.gene040735.t1
chr1.2 CDS 35149527 35149683 35149527 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35149773 35149992 35149773 ID=MS.gene040735.t1.exon3;Parent=MS.gene040735.t1
chr1.2 CDS 35149773 35149992 35149773 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35150156 35150209 35150156 ID=MS.gene040735.t1.exon4;Parent=MS.gene040735.t1
chr1.2 CDS 35150156 35150209 35150156 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35150295 35150374 35150295 ID=MS.gene040735.t1.exon5;Parent=MS.gene040735.t1
chr1.2 CDS 35150295 35150374 35150295 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35150461 35150738 35150461 ID=MS.gene040735.t1.exon6;Parent=MS.gene040735.t1
chr1.2 CDS 35150461 35150738 35150461 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35151225 35151623 35151225 ID=MS.gene040735.t1.exon7;Parent=MS.gene040735.t1
chr1.2 CDS 35151225 35151623 35151225 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35151720 35151895 35151720 ID=MS.gene040735.t1.exon8;Parent=MS.gene040735.t1
chr1.2 CDS 35151720 35151895 35151720 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
chr1.2 exon 35152070 35152342 35152070 ID=MS.gene040735.t1.exon9;Parent=MS.gene040735.t1
chr1.2 CDS 35152070 35152342 35152070 ID=cds.MS.gene040735.t1;Parent=MS.gene040735.t1
Gene Sequence

>MS.gene040735

ATGACGAACATTACTCAAGAATGTCCAACGTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCCCCCGAGGCTTCTAAATTTGGTATGTAATAACAGATTATGTATTGTTTTCTTTTCATCACCATGTTTCTAGGGTTTTTTTATCTGTCATTAAACAAAGTTATGTAACATTGCTGTTTGTCTTTCCAAGTATAGTAAGAATTCTACTTAATGTAACTTCCTTTTATCCCTCAACTTTCTTTTGCATCCATCTCTGAAGTGCTCGAGTTTACGTGCATGTTTAGATTAACGGTGAATCTGCAAAATTACTGTAACAAAGTGATACTTGAAAATATAGATTTTACTAAAATCACAGTGGCACGCTATGATTTGTCAGAACGATATAATCTAATATAGGACAAACTTGTCTGCAACAGATTCTGCATTTTTAGGTCTTTTTTTCTTTTCTTTTCTGTTTGTATGCTTATATTTGATCTTAATATTTCAGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAATGGAAGAGAAAAAGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCGAATCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAAAGGGTAAGAAAGAACAAAGTTTTCTTAAGTTTTTTAAACAACTTGAATGATGAAAAATTAACTAACTTGGTTTTTGATATTGTTTGTACTAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCGTTTTCCAATAGATTGCGTGGTAAAGAAGATCTCTCTTGTTCAGATATCGAAAAAGCGTTCTGGCACGAAATAGTACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATGTTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGGTTTCTTTTCAAAATTTATGTTTGCTAAATTCATTGTTAGTTTTGTCTTGACATCTTTAACTATTGAGTTTATATTTGAATTCAGTCTATTAATCTTTACTTGTAGCACTATTCTTCTGTAAGTGTAATTATTGTTTGATTAACACTTTTCATTTGATTTTAGAATTTGCCGCGGCTGCCACAGTCTCCATTGCGTTTAGTTGACAGGAAAATTCCAGTAAGGAAGCAATTTTTGTAACCTAATATGAATTAAAAGTTGTTAATTATTATTATTATTATTTTAATTTTATTCAAAATTTCAGGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGGTATATTATCTTAACATTGCTTCAAATATTGTTTAACATGCTGATATATCATCATGTCTTAATATATAGTACTGAATTTTTTGCAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCATCCTCTCAATTTGAAAAGACTGTCTTGAATCATGTGTATTGCAACAAAGTCTTAAAAGAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCGTCACCTTTCCAAATTCATATCATGCTGGATTTAGTCATGGTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGATCTCTCTCTACATGTGTTGGTGTTATTACGTTTCTGATGGATATTGCTTATATTTTACGAATAAGTAGGCAGGTTGATTGAATAATAGAAAGGTACGTAGGTTTAAAGGTTAATCTTGTAAACCCTAAGCTTGAATAATAGAAAGGTACTTCCGCCACTATGCAGCAGCAGAGGAACAATAGGCCGAACTCCATAATCAAATCTCGCGTGTTCTCTGTAATCTGTTTATTTTTGTACAAGGCTTGAAGTTCTAATAGTTACTACATATAGAGACATCTATTTATTGTTCTCATTGAATTTTTTTTCCATAATAATACCTGGCGGAATTTCACTTCCTGCTATTTTAAATACTTATGTATCTTACTTGATGTGCAAGTTAGTAATCTAGTACAAAAGCTAAAGATAGTATTAGTAACTAATTATTGAATCATTTTCTTGTTGAGTTTAGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTCGGGGCTGCAGCTAGCAAACGTTATTCACATCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTGACAAAATCAAACTTGAAGATATGGCAACTTATCGAGCTATTGCACTATCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCGGTTGGGCAGTTCGAGAAAGTTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCAATAGGGATTGTTATGTAGCTTTCTTCTTGTGCAAGAAATGCTATTCAGATCCAATTTGCCTTTTCCATGGTAAGACCTTGTTATTCTCTTTGAGTATTTCAGAAATTGTTATTTAAGCTGCTTGTGTTATTGATGGATATACTTGCTTATTATGGTTATATTCAGACAATGTGCCACAAAATTGTTTATGCGGGAGAGAACATACTGTATTTAAGAGGAATGACATGTTGGAACTCGAAGATGCTGCCAAAAGTCTTCAACAGGAAAAAGAATACACCAACGCGTTACCTTTAACAAATATATTCTCCTATAACATAAATGAATGCATCAAAGAACACAAGGTATGTGAAAGTCTAAACTATGATATTGATTTACAATTTAGAATCCTCTAAACTTCATTATAATAGTCGCATTTGATTGATTACAATACAAGTATAACACATTGCTTTATCTTCAGGTTGCTTATTTTATTGTATCTGATTGATTTTAACTTTTGGAATTAAAACTGAAAACAGAATAATAATTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCACTAGGTGCAACATCTAAACTGAAAACAAGAGCTGCGAACAACATCAAGCTCACTGTTGAAAATAATCAAAAGACAAAGAATCGTTGTAGAAACAATGTGTCTCCATCAGTGATGAGTACCAAAAGACAACGAGTCCTTTATAACTTGAGAAAACATGAATCGGAACTGATTACCAAAGTTCCTTCAATATCTTCCCTTTTGGCGACATCTGAAAAATCAAATTAA
Protein sequence

>MS.gene040735.t1

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKFGICKIVSPIAASNPASFVLMEEKKDFKFNTIVQPLRVFESNEMDKITFSKRKGKYTYHEFEALANKAFSNRLRGKEDLSCSDIEKAFWHEIVHGEKGTVEYGVNVEESAFSSDPDDKLGTSNFNLKNLPRLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSASSQFEKTVLNHVYCNKVLKEHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKAVQKPGEFVVTFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASKRYSHLKMLPIIPYEELLCKEALLIYNSSKARGDKIKLEDMATYRAIALSFLHLIQFYKTSLSRLGSSRKLSSSSNTSLGSVTCSLCNRDCYVAFFLCKKCYSDPICLFHDNVPQNCLCGREHTVFKRNDMLELEDAAKSLQQEKEYTNALPLTNIFSYNINECIKEHKNNNWHEEKSARRTVNSLGATSKLKTRAANNIKLTVENNQKTKNRCRNNVSPSVMSTKRQRVLYNLRKHESELITKVPSISSLLATSEKSN