AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040802

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040802.t1 MTR_1g047060 48.454 97 43 2 112 208 3 92 1.71e-22 89.0
MS.gene040802.t1 MTR_6g477900 32.738 168 90 8 4 157 6 164 2.97e-18 81.6
MS.gene040802.t1 MTR_4g094302 31.551 187 103 9 4 176 11 186 6.12e-18 79.7
MS.gene040802.t1 MTR_8g076110 27.948 229 143 8 4 216 6 228 4.71e-17 79.3
MS.gene040802.t1 MTR_4g101680 32.704 159 84 7 4 148 7 156 1.20e-16 79.0
MS.gene040802.t1 MTR_5g021710 31.210 157 91 5 4 149 8 158 1.41e-16 78.6
MS.gene040802.t1 MTR_5g012080 32.184 174 93 9 4 159 7 173 2.20e-16 78.2
MS.gene040802.t1 MTR_1g008740 32.716 162 91 6 2 151 25 180 4.30e-16 77.4
MS.gene040802.t1 MTR_1g008740 32.716 162 91 6 2 151 11 166 5.45e-16 77.0
MS.gene040802.t1 MTR_8g023900 30.625 160 90 7 2 148 10 161 1.84e-15 75.9
MS.gene040802.t1 MTR_8g023900 28.272 191 112 8 7 184 196 374 1.29e-13 70.5
MS.gene040802.t1 MTR_4g094302 33.333 129 77 6 4 132 11 130 1.84e-15 72.4
MS.gene040802.t1 MTR_4g036030 32.051 156 89 6 4 148 7 156 2.01e-15 75.1
MS.gene040802.t1 MTR_3g093050 30.952 168 84 9 1 149 19 173 2.46e-15 75.9
MS.gene040802.t1 MTR_2g093810 32.143 168 88 8 4 154 14 172 2.71e-15 74.7
MS.gene040802.t1 MTR_3g093050 30.952 168 84 9 1 149 19 173 2.83e-15 75.5
MS.gene040802.t1 MTR_3g093040 30.909 165 82 9 4 149 108 259 3.03e-15 74.3
MS.gene040802.t1 MTR_5g041940 29.630 162 88 6 4 148 3 155 3.30e-15 73.6
MS.gene040802.t1 MTR_5g069030 31.548 168 83 9 1 149 22 176 6.50e-15 74.3
MS.gene040802.t1 MTR_2g080010 29.762 168 102 6 4 164 15 173 7.97e-15 73.6
MS.gene040802.t1 MTR_8g023930 28.814 177 107 7 2 167 22 190 1.76e-14 73.2
MS.gene040802.t1 MTR_4g089135 32.353 136 79 4 4 133 15 143 5.28e-14 71.2
MS.gene040802.t1 MTR_7g100990 34.815 135 75 6 4 132 11 138 5.75e-14 70.9
MS.gene040802.t1 MTR_3g109340 30.189 159 92 6 2 148 20 171 1.06e-13 70.5
MS.gene040802.t1 MTR_8g059170 30.247 162 87 7 4 149 14 165 2.00e-13 69.3
MS.gene040802.t1 MTR_3g096140 30.435 161 88 7 3 148 6 157 2.42e-13 68.6
MS.gene040802.t1 MTR_2g079990 29.375 160 89 8 4 149 16 165 3.37e-13 68.9
MS.gene040802.t1 MTR_3g096140 30.435 161 88 7 3 148 6 157 5.91e-13 68.2
MS.gene040802.t1 MTR_3g088110 29.630 162 86 7 4 148 7 157 6.81e-13 67.4
MS.gene040802.t1 MTR_6g084430 31.410 156 87 7 4 148 5 151 8.42e-13 67.4
MS.gene040802.t1 MTR_1g090720 32.121 165 86 6 4 149 42 199 1.16e-12 67.8
MS.gene040802.t1 MTR_7g085220 26.816 179 98 7 4 157 8 178 1.24e-12 67.0
MS.gene040802.t1 MTR_8g467490 27.647 170 92 7 2 148 12 173 2.06e-12 66.6
MS.gene040802.t1 MTR_4g098630 30.435 161 91 9 4 149 6 160 2.57e-12 65.9
MS.gene040802.t1 MTR_7g005280 30.128 156 92 6 4 150 15 162 3.09e-12 65.1
MS.gene040802.t1 MTR_4g108760 30.625 160 87 8 4 148 17 167 3.98e-12 65.9
MS.gene040802.t1 MTR_7g097090 32.026 153 86 7 4 146 10 154 6.90e-12 64.7
MS.gene040802.t1 MTR_1g097300 34.074 135 72 5 6 132 21 146 6.93e-12 65.5
MS.gene040802.t1 MTR_2g079990 31.655 139 78 7 4 135 16 144 8.02e-12 65.1
MS.gene040802.t1 MTR_3g435150 31.677 161 86 9 3 148 15 166 3.18e-11 62.8
MS.gene040802.t1 MTR_4g075980 28.947 152 92 6 6 148 17 161 4.21e-11 62.4
MS.gene040802.t1 MTR_8g093580 28.750 160 88 7 6 149 8 157 6.49e-11 62.4
MS.gene040802.t1 MTR_3g098810 28.144 167 92 7 4 150 14 172 6.91e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040802.t1 AT4G36160 31.977 172 98 6 4 162 10 175 3.01e-19 86.3
MS.gene040802.t1 AT4G36160 31.977 172 98 6 4 162 10 175 3.01e-19 86.3
MS.gene040802.t1 AT4G36160 31.977 172 98 6 4 162 14 179 3.36e-19 86.3
MS.gene040802.t1 AT2G18060 32.164 171 98 6 4 162 9 173 7.62e-19 85.1
MS.gene040802.t1 AT2G18060 32.164 171 98 6 4 162 9 173 7.62e-19 85.1
MS.gene040802.t1 AT3G17730 32.500 160 86 8 4 149 6 157 3.79e-18 81.6
MS.gene040802.t1 AT5G62380 30.542 203 111 8 4 192 7 193 6.41e-18 82.4
MS.gene040802.t1 AT5G62380 30.542 203 111 8 4 192 7 193 6.41e-18 82.4
MS.gene040802.t1 AT2G24430 33.962 159 86 6 2 148 14 165 6.57e-17 79.3
MS.gene040802.t1 AT2G24430 33.962 159 86 6 2 148 14 165 6.57e-17 79.3
MS.gene040802.t1 AT1G71930 30.769 156 91 5 4 148 9 158 1.82e-16 78.2
MS.gene040802.t1 AT1G71930 30.769 156 91 5 4 148 9 158 1.82e-16 78.2
MS.gene040802.t1 AT1G12260 33.125 160 84 8 4 149 7 157 3.78e-16 77.4
MS.gene040802.t1 AT3G10490 31.875 160 88 6 4 149 27 179 4.93e-16 75.5
MS.gene040802.t1 AT1G76420 32.919 161 81 8 4 148 22 171 5.80e-16 76.6
MS.gene040802.t1 AT1G12260 33.333 159 83 8 4 148 7 156 7.45e-16 77.0
MS.gene040802.t1 AT1G62700 32.075 159 85 7 4 148 7 156 1.62e-15 75.9
MS.gene040802.t1 AT1G62700 32.075 159 85 7 4 148 7 156 1.62e-15 75.9
MS.gene040802.t1 AT1G54330 30.128 156 92 6 4 149 6 154 2.85e-15 74.3
MS.gene040802.t1 AT3G10490 31.875 160 88 6 4 149 27 179 4.63e-15 74.7
MS.gene040802.t1 AT5G64530 29.897 194 102 6 4 176 3 183 4.85e-15 71.6
MS.gene040802.t1 AT3G10480 31.875 160 88 6 4 149 27 179 1.28e-14 73.6
MS.gene040802.t1 AT4G27410 28.652 178 88 8 4 151 14 182 2.77e-14 71.6
MS.gene040802.t1 AT3G49530 28.144 167 91 9 1 149 10 165 3.18e-14 72.0
MS.gene040802.t1 AT3G49530 28.144 167 91 9 1 149 10 165 3.50e-14 72.0
MS.gene040802.t1 AT5G39610 31.677 161 86 8 4 149 20 171 4.34e-14 70.9
MS.gene040802.t1 AT3G10480 31.447 159 89 6 4 149 27 178 4.34e-14 72.0
MS.gene040802.t1 AT5G46590 31.210 157 86 7 4 145 6 155 5.42e-14 70.9
MS.gene040802.t1 AT1G32510 28.743 167 93 7 3 149 5 165 6.80e-14 70.5
MS.gene040802.t1 AT5G24590 29.268 164 87 8 4 149 13 165 1.77e-13 70.1
MS.gene040802.t1 AT5G64530 34.884 129 71 5 4 132 3 118 2.44e-13 66.6
MS.gene040802.t1 AT5G04395 31.544 149 81 5 4 135 28 172 2.52e-13 67.0
MS.gene040802.t1 AT3G15500 28.931 159 91 7 4 149 14 163 3.76e-13 68.6
MS.gene040802.t1 AT3G18400 31.847 157 86 9 4 148 5 152 4.01e-13 68.6
MS.gene040802.t1 AT1G26870 29.348 184 95 8 2 162 14 185 4.25e-13 68.9
MS.gene040802.t1 AT5G22290 30.405 148 86 7 6 145 23 161 4.73e-13 68.6
MS.gene040802.t1 AT4G35580 27.869 183 102 8 1 163 6 178 6.45e-13 68.6
MS.gene040802.t1 AT4G35580 27.869 183 102 8 1 163 6 178 6.82e-13 68.6
MS.gene040802.t1 AT4G35580 27.869 183 102 8 1 163 6 178 6.83e-13 68.6
MS.gene040802.t1 AT5G61430 31.288 163 88 8 4 151 16 169 6.85e-13 67.8
MS.gene040802.t1 AT1G77450 28.571 182 97 7 5 164 11 181 7.32e-13 67.0
MS.gene040802.t1 AT1G33060 28.313 166 85 8 1 146 21 172 8.19e-13 68.2
MS.gene040802.t1 AT1G33060 28.313 166 85 8 1 146 21 172 8.29e-13 68.2
MS.gene040802.t1 AT3G10500 31.013 158 89 6 4 148 9 159 8.57e-13 68.2
MS.gene040802.t1 AT1G52890 29.448 163 85 7 4 149 14 163 1.08e-12 67.0
MS.gene040802.t1 AT3G29035 33.333 135 75 6 4 132 24 149 1.19e-12 67.0
MS.gene040802.t1 AT4G10350 31.410 156 88 7 4 146 9 158 1.35e-12 67.0
MS.gene040802.t1 AT5G04410 29.114 158 92 6 4 148 9 159 2.40e-12 67.0
MS.gene040802.t1 AT5G46590 30.935 139 82 6 4 135 6 137 5.36e-12 64.7
MS.gene040802.t1 AT5G07680 31.288 163 88 8 4 151 17 170 6.90e-12 65.1
MS.gene040802.t1 AT5G50820 33.088 136 76 5 4 135 17 141 7.63e-12 63.2
MS.gene040802.t1 AT5G07680 31.288 163 88 8 4 151 3 156 7.75e-12 64.7
MS.gene040802.t1 AT3G04060 29.508 183 104 8 4 170 20 193 8.62e-12 64.7
MS.gene040802.t1 AT3G15510 27.778 162 101 6 10 164 23 175 1.20e-11 64.3
MS.gene040802.t1 AT3G12977 27.072 181 98 8 4 164 18 184 4.84e-11 62.0

Find 50 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTTAAGCTTGCAATTA+TGG 0.141545 1.2:+19275240 MS.gene040802:CDS
CCATGGTTCTGACTTGATTA+AGG 0.237815 1.2:+19273791 MS.gene040802:CDS
GGCATTGTAAGACTGGATTT+TGG 0.240614 1.2:+19273925 MS.gene040802:CDS
CCTCCTTAGCTCATCACTTT+TGG 0.317816 1.2:-19273904 None:intergenic
CCTTGTGTGAGGGAAGAAAA+AGG 0.370691 1.2:-19273862 None:intergenic
TGAAGGAACTCAACAACTTA+AGG 0.384542 1.2:+19274336 MS.gene040802:CDS
GCAATTCAAACGGATCATAT+TGG 0.403266 1.2:-19273827 None:intergenic
AGGTGATGCATTGCATTTCA+TGG 0.410069 1.2:+19274399 MS.gene040802:CDS
CGTTTGAATTGCATCTTGCT+CGG 0.448065 1.2:+19273838 MS.gene040802:CDS
AAAACACGGCAAACAACAAA+AGG 0.449123 1.2:-19274214 None:intergenic
TGTCCAGATCCATCCCTCTA+TGG 0.461584 1.2:-19274243 None:intergenic
CTAAGGAGGCATTGTAAGAC+TGG 0.467155 1.2:+19273918 MS.gene040802:CDS
GAAGGAACTCAACAACTTAA+GGG 0.473282 1.2:+19274337 MS.gene040802:CDS
CCGCCATAGAGGGATGGATC+TGG 0.481918 1.2:+19274240 MS.gene040802:CDS
GAAGAGGTTCAAAATTTCCA+TGG 0.507513 1.2:+19273774 MS.gene040802:CDS
GGATCTGGACACTGAATTGT+GGG 0.513521 1.2:+19274255 MS.gene040802:CDS
TGGATCTGGACACTGAATTG+TGG 0.515403 1.2:+19274254 MS.gene040802:CDS
TCTGATGAGAAGGCGAATGA+AGG 0.523662 1.2:+19274319 MS.gene040802:CDS
ATTGCAAGCTTAAATTGCTC+CGG 0.527174 1.2:-19275235 None:intergenic
GATTTGCTTCCACCTGGTGT+TGG 0.532322 1.2:+19273696 MS.gene040802:CDS
ACCTTCATCGACATCAGATA+AGG 0.533255 1.2:-19274278 None:intergenic
AGAACGCAATCAAAACTAGC+TGG 0.537621 1.2:+19274045 MS.gene040802:CDS
TGGAGCTGATGATGTGTTGG+TGG 0.544087 1.2:+19273980 MS.gene040802:CDS
CGAGCAAGATGCAATTCAAA+CGG 0.545354 1.2:-19273837 None:intergenic
CACTCTTGTTAACAACCCTC+AGG 0.546751 1.2:+19274082 MS.gene040802:CDS
CCACGTGGAAGCTATGACGC+CGG 0.547299 1.2:+19275216 MS.gene040802:CDS
GTCGATGAAGGTGATGTTGT+TGG 0.547764 1.2:+19274289 MS.gene040802:CDS
TGTTGGAGCTGATGATGTGT+TGG 0.549550 1.2:+19273977 MS.gene040802:CDS
GCTGTCGAAGACATTACTGT+TGG 0.561036 1.2:+19273960 MS.gene040802:CDS
TAGCAAGGTAGTAGCTAACA+AGG 0.569555 1.2:-19273740 None:intergenic
GCAATGGATTTGCTTCCACC+TGG 0.569568 1.2:+19273690 None:intergenic
CCAAAAGTGATGAGCTAAGG+AGG 0.576383 1.2:+19273904 MS.gene040802:CDS
CAGTAAAACTCCCTTGTGTG+AGG 0.577220 1.2:-19273873 None:intergenic
CTGCCAAAAGTGATGAGCTA+AGG 0.578064 1.2:+19273901 MS.gene040802:CDS
AAGGAACTCAACAACTTAAG+GGG 0.586043 1.2:+19274338 MS.gene040802:CDS
GCCTTATCTGATGTCGATGA+AGG 0.592468 1.2:+19274277 MS.gene040802:CDS
AGTAAAACTCCCTTGTGTGA+GGG 0.603789 1.2:-19273872 None:intergenic
GGAGCTGATGATGTGTTGGT+GGG 0.610668 1.2:+19273981 MS.gene040802:CDS
TAACAAGGAGTTCATCAGAA+GGG 0.633655 1.2:-19273725 None:intergenic
CCGGCGTCATAGCTTCCACG+TGG 0.637025 1.2:-19275216 None:intergenic
CCTTAATCAAGTCAGAACCA+TGG 0.642289 1.2:-19273791 None:intergenic
GGGACGAAACCAACACCAGG+TGG 0.656589 1.2:-19273705 None:intergenic
GCTGATGCTAGTGCTGAGCA+AGG 0.658942 1.2:+19274379 MS.gene040802:CDS
TGGTGACGACTCTGATGAGA+AGG 0.667599 1.2:+19274309 MS.gene040802:CDS
CTAACAAGGAGTTCATCAGA+AGG 0.675712 1.2:-19273726 None:intergenic
CCAGATCCATCCCTCTATGG+CGG 0.685304 1.2:-19274240 None:intergenic
GAAGGGACGAAACCAACACC+AGG 0.689839 1.2:-19273708 None:intergenic
AGGAACTCAACAACTTAAGG+GGG 0.701895 1.2:+19274339 MS.gene040802:CDS
TGATTGTGTTGTGTCCCACG+TGG 0.721065 1.2:+19275201 MS.gene040802:CDS
CGGCGTCATAGCTTCCACGT+GGG 0.736879 1.2:-19275215 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAAAGAAAAATAAAA+AGG - chr1.2:19275023-19275042 None:intergenic 10.0%
!! TCAAAAATTATGTTTAATTA+CGG + chr1.2:19274747-19274766 MS.gene040802:intron 10.0%
!! TTTAATATAAATAATTGTGT+AGG + chr1.2:19274189-19274208 MS.gene040802:intron 10.0%
!!! TTAATTTTTAACATGTTTTA+AGG - chr1.2:19274956-19274975 None:intergenic 10.0%
!! CATAAAAATCATTAATATTG+TGG - chr1.2:19274561-19274580 None:intergenic 15.0%
!!! TATTTTTCTTTAAGTTTTTG+TGG + chr1.2:19275026-19275045 MS.gene040802:intron 15.0%
!! GTTTGATATTGATATTTATC+AGG + chr1.2:19275159-19275178 MS.gene040802:intron 20.0%
!! TCGATGATTAATTTATGTAT+TGG + chr1.2:19274149-19274168 MS.gene040802:intron 20.0%
!!! AATATATTTTACCTGAAAAG+TGG - chr1.2:19274434-19274453 None:intergenic 20.0%
!!! CATAATTTTTGATACAATTG+TGG - chr1.2:19274739-19274758 None:intergenic 20.0%
! CATAAATAATCCAAACACAA+TGG - chr1.2:19274685-19274704 None:intergenic 25.0%
! TATTCAAAACCTTTATGTGT+TGG + chr1.2:19274624-19274643 MS.gene040802:intron 25.0%
! TTAATTACGGAAGTCAATAT+TGG + chr1.2:19274760-19274779 MS.gene040802:intron 25.0%
!! AATAAAAAGGACATGTTTTG+AGG - chr1.2:19275010-19275029 None:intergenic 25.0%
!! CATCTTTTCGTGTAATTTTA+TGG + chr1.2:19275097-19275116 MS.gene040802:intron 25.0%
!! GGATGTAGAATTTTGTAATT+GGG - chr1.2:19274718-19274737 None:intergenic 25.0%
!! TATGACCAACTTTTAAATCA+TGG + chr1.2:19274585-19274604 MS.gene040802:intron 25.0%
!! TCTCTCTTCTTTTTCTATAT+AGG + chr1.2:19274011-19274030 MS.gene040802:CDS 25.0%
!! TGGATGTAGAATTTTGTAAT+TGG - chr1.2:19274719-19274738 None:intergenic 25.0%
!!! CTCGATATTTATTTTGTCAT+CGG + chr1.2:19274466-19274485 MS.gene040802:intron 25.0%
CAAAAACTACCAACACATAA+AGG - chr1.2:19274636-19274655 None:intergenic 30.0%
GCAATTTAAGCTTGCAATTA+TGG + chr1.2:19275240-19275259 MS.gene040802:CDS 30.0%
TGCTAACAAAAACAATGAAG+AGG + chr1.2:19273758-19273777 MS.gene040802:CDS 30.0%
TGTAAAATTCAGTTGCAAAG+AGG - chr1.2:19274811-19274830 None:intergenic 30.0%
TGTGACCATGATTTAAAAGT+TGG - chr1.2:19274593-19274612 None:intergenic 30.0%
TTAAATCATGGTCACACTAT+AGG + chr1.2:19274597-19274616 MS.gene040802:intron 30.0%
! CTTTTCGTGTAATTTTATGG+TGG + chr1.2:19275100-19275119 MS.gene040802:intron 30.0%
!!! CTTCATTGTTTTTGTTAGCA+AGG - chr1.2:19273758-19273777 None:intergenic 30.0%
!!! TTATGTGTTGGTAGTTTTTG+TGG + chr1.2:19274636-19274655 MS.gene040802:intron 30.0%
AAAACACGGCAAACAACAAA+AGG - chr1.2:19274217-19274236 None:intergenic 35.0%
AAAATTCCTCCATCATATGC+GGG + chr1.2:19274535-19274554 MS.gene040802:intron 35.0%
AAGGAACTCAACAACTTAAG+GGG + chr1.2:19274338-19274357 MS.gene040802:CDS 35.0%
AGAGGATCAACAACATTAAC+TGG - chr1.2:19274793-19274812 None:intergenic 35.0%
ATTGCAAGCTTAAATTGCTC+CGG - chr1.2:19275238-19275257 None:intergenic 35.0%
CAAAATTCCTCCATCATATG+CGG + chr1.2:19274534-19274553 MS.gene040802:intron 35.0%
GAAGAGGTTCAAAATTTCCA+TGG + chr1.2:19273774-19273793 MS.gene040802:CDS 35.0%
GAAGGAACTCAACAACTTAA+GGG + chr1.2:19274337-19274356 MS.gene040802:CDS 35.0%
GCAATTCAAACGGATCATAT+TGG - chr1.2:19273830-19273849 None:intergenic 35.0%
TAACAAGGAGTTCATCAGAA+GGG - chr1.2:19273728-19273747 None:intergenic 35.0%
TGAAGGAACTCAACAACTTA+AGG + chr1.2:19274336-19274355 MS.gene040802:CDS 35.0%
!!! TAGTTTTGATTGCGTTCTGA+GGG - chr1.2:19274043-19274062 None:intergenic 35.0%
AATCATGGTCACACTATAGG+TGG + chr1.2:19274600-19274619 MS.gene040802:intron 40.0%
ACCTTCATCGACATCAGATA+AGG - chr1.2:19274281-19274300 None:intergenic 40.0%
AGAACGCAATCAAAACTAGC+TGG + chr1.2:19274045-19274064 MS.gene040802:CDS 40.0%
AGGAACTCAACAACTTAAGG+GGG + chr1.2:19274339-19274358 MS.gene040802:CDS 40.0%
AGGTGATGCATTGCATTTCA+TGG + chr1.2:19274399-19274418 MS.gene040802:CDS 40.0%
AGTAAAACTCCCTTGTGTGA+GGG - chr1.2:19273875-19273894 None:intergenic 40.0%
CCAAACACAATGGAGCTATT+TGG - chr1.2:19274675-19274694 None:intergenic 40.0%
CCAAATAGCTCCATTGTGTT+TGG + chr1.2:19274672-19274691 MS.gene040802:intron 40.0%
CCTTAATCAAGTCAGAACCA+TGG - chr1.2:19273794-19273813 None:intergenic 40.0%
CGAGCAAGATGCAATTCAAA+CGG - chr1.2:19273840-19273859 None:intergenic 40.0%
CGTTTGAATTGCATCTTGCT+CGG + chr1.2:19273838-19273857 MS.gene040802:CDS 40.0%
CTAACAAGGAGTTCATCAGA+AGG - chr1.2:19273729-19273748 None:intergenic 40.0%
TAGCAAGGTAGTAGCTAACA+AGG - chr1.2:19273743-19273762 None:intergenic 40.0%
TTCCCAAATGACATACCTGA+GGG - chr1.2:19274100-19274119 None:intergenic 40.0%
TTTCCCAAATGACATACCTG+AGG - chr1.2:19274101-19274120 None:intergenic 40.0%
! AACCCTCAGGTATGTCATTT+GGG + chr1.2:19274095-19274114 MS.gene040802:intron 40.0%
! ACTGGATTTTGGATGCAGAT+GGG + chr1.2:19273936-19273955 MS.gene040802:CDS 40.0%
! CTTTTTCTTCCCTCACACAA+GGG + chr1.2:19273863-19273882 MS.gene040802:CDS 40.0%
! GGCATTGTAAGACTGGATTT+TGG + chr1.2:19273925-19273944 MS.gene040802:CDS 40.0%
!! CCATGGTTCTGACTTGATTA+AGG + chr1.2:19273791-19273810 MS.gene040802:CDS 40.0%
!!! AGGACATGTTTTGAGGAATC+AGG - chr1.2:19275003-19275022 None:intergenic 40.0%
!!! AGTTTTGATTGCGTTCTGAG+GGG - chr1.2:19274042-19274061 None:intergenic 40.0%
!!! CTAGTTTTGATTGCGTTCTG+AGG - chr1.2:19274044-19274063 None:intergenic 40.0%
ACTCAACAACTTAAGGGGGT+TGG + chr1.2:19274343-19274362 MS.gene040802:CDS 45.0%
ATATTGTGGCCCGCATATGA+TGG - chr1.2:19274547-19274566 None:intergenic 45.0%
CAACCCTCAGGTATGTCATT+TGG + chr1.2:19274094-19274113 MS.gene040802:intron 45.0%
CACTCTTGTTAACAACCCTC+AGG + chr1.2:19274082-19274101 MS.gene040802:CDS 45.0%
CAGTAAAACTCCCTTGTGTG+AGG - chr1.2:19273876-19273895 None:intergenic 45.0%
CCAAAAGTGATGAGCTAAGG+AGG + chr1.2:19273904-19273923 MS.gene040802:CDS 45.0%
CCTTGTGTGAGGGAAGAAAA+AGG - chr1.2:19273865-19273884 None:intergenic 45.0%
CTCAACAACTTAAGGGGGTT+GGG + chr1.2:19274344-19274363 MS.gene040802:CDS 45.0%
CTCTATGGCGGTCAAAAACA+CGG - chr1.2:19274231-19274250 None:intergenic 45.0%
CTGCCAAAAGTGATGAGCTA+AGG + chr1.2:19273901-19273920 MS.gene040802:CDS 45.0%
GCCTTATCTGATGTCGATGA+AGG + chr1.2:19274277-19274296 MS.gene040802:CDS 45.0%
GCTGTCGAAGACATTACTGT+TGG + chr1.2:19273960-19273979 MS.gene040802:CDS 45.0%
GGATCTGGACACTGAATTGT+GGG + chr1.2:19274255-19274274 MS.gene040802:CDS 45.0%
GTCGATGAAGGTGATGTTGT+TGG + chr1.2:19274289-19274308 MS.gene040802:CDS 45.0%
TCTGATGAGAAGGCGAATGA+AGG + chr1.2:19274319-19274338 MS.gene040802:CDS 45.0%
TGGATCTGGACACTGAATTG+TGG + chr1.2:19274254-19274273 MS.gene040802:CDS 45.0%
! CCTCCTTAGCTCATCACTTT+TGG - chr1.2:19273907-19273926 None:intergenic 45.0%
! CCTTTTTCTTCCCTCACACA+AGG + chr1.2:19273862-19273881 MS.gene040802:CDS 45.0%
! GACTGGATTTTGGATGCAGA+TGG + chr1.2:19273935-19273954 MS.gene040802:CDS 45.0%
! TGTTGGAGCTGATGATGTGT+TGG + chr1.2:19273977-19273996 MS.gene040802:CDS 45.0%
!! CTAAGGAGGCATTGTAAGAC+TGG + chr1.2:19273918-19273937 MS.gene040802:CDS 45.0%
!! GTGTTTTTGACCGCCATAGA+GGG + chr1.2:19274230-19274249 MS.gene040802:CDS 45.0%
TGATTGTGTTGTGTCCCACG+TGG + chr1.2:19275201-19275220 MS.gene040802:CDS 50.0%
TGTCCAGATCCATCCCTCTA+TGG - chr1.2:19274246-19274265 None:intergenic 50.0%
! GATTTGCTTCCACCTGGTGT+TGG + chr1.2:19273696-19273715 MS.gene040802:CDS 50.0%
! GGAGCTGATGATGTGTTGGT+GGG + chr1.2:19273981-19274000 MS.gene040802:CDS 50.0%
! TGGAGCTGATGATGTGTTGG+TGG + chr1.2:19273980-19273999 MS.gene040802:CDS 50.0%
! TGGTGACGACTCTGATGAGA+AGG + chr1.2:19274309-19274328 MS.gene040802:CDS 50.0%
! TTTTGACCGCCATAGAGGGA+TGG + chr1.2:19274234-19274253 MS.gene040802:CDS 50.0%
!! CGTGTTTTTGACCGCCATAG+AGG + chr1.2:19274229-19274248 MS.gene040802:CDS 50.0%
!!! GGCTACTCAAGCCACTTTTC+AGG + chr1.2:19274420-19274439 MS.gene040802:CDS 50.0%
CCAGATCCATCCCTCTATGG+CGG - chr1.2:19274243-19274262 None:intergenic 55.0%
GAAGGGACGAAACCAACACC+AGG - chr1.2:19273711-19273730 None:intergenic 55.0%
TTGTGGCCCGCATATGATGG+AGG - chr1.2:19274544-19274563 None:intergenic 55.0%
! GCTGATGCTAGTGCTGAGCA+AGG + chr1.2:19274379-19274398 MS.gene040802:CDS 55.0%
CCACGTGGAAGCTATGACGC+CGG + chr1.2:19275216-19275235 MS.gene040802:CDS 60.0%
CCGCCATAGAGGGATGGATC+TGG + chr1.2:19274240-19274259 MS.gene040802:CDS 60.0%
CGGCGTCATAGCTTCCACGT+GGG - chr1.2:19275218-19275237 None:intergenic 60.0%
GGGACGAAACCAACACCAGG+TGG - chr1.2:19273708-19273727 None:intergenic 60.0%
CCGGCGTCATAGCTTCCACG+TGG - chr1.2:19275219-19275238 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 19273693 19275279 19273693 ID=MS.gene040802
chr1.2 mRNA 19273693 19275279 19273693 ID=MS.gene040802.t1;Parent=MS.gene040802
chr1.2 exon 19273693 19274103 19273693 ID=MS.gene040802.t1.exon1;Parent=MS.gene040802.t1
chr1.2 CDS 19273693 19274103 19273693 ID=cds.MS.gene040802.t1;Parent=MS.gene040802.t1
chr1.2 exon 19274211 19274441 19274211 ID=MS.gene040802.t1.exon2;Parent=MS.gene040802.t1
chr1.2 CDS 19274211 19274441 19274211 ID=cds.MS.gene040802.t1;Parent=MS.gene040802.t1
chr1.2 exon 19274713 19274757 19274713 ID=MS.gene040802.t1.exon3;Parent=MS.gene040802.t1
chr1.2 CDS 19274713 19274757 19274713 ID=cds.MS.gene040802.t1;Parent=MS.gene040802.t1
chr1.2 exon 19275181 19275279 19275181 ID=MS.gene040802.t1.exon4;Parent=MS.gene040802.t1
chr1.2 CDS 19275181 19275279 19275181 ID=cds.MS.gene040802.t1;Parent=MS.gene040802.t1
Gene Sequence

>MS.gene040802

ATGGATTTGCTTCCACCTGGTGTTGGTTTCGTCCCTTCTGATGAACTCCTTGTTAGCTACTACCTTGCTAACAAAAACAATGAAGAGGTTCAAAATTTCCATGGTTCTGACTTGATTAAGGAGCTAAACATGTACCAATATGATCCGTTTGAATTGCATCTTGCTCGGTCCTTTTTCTTCCCTCACACAAGGGAGTTTTACTGCTTCACTGCCAAAAGTGATGAGCTAAGGAGGCATTGTAAGACTGGATTTTGGATGCAGATGGGAGCTGTCGAAGACATTACTGTTGGAGCTGATGATGTGTTGGTGGGAAAGAAGTCTCTCTTCTTTTTCTATATAGGAAATTCCCCTCAGAACGCAATCAAAACTAGCTGGACTATGTACGAGTACACTCTTGTTAACAACCCTCAGGTATGTCATTTGGGAAAACTAACTTTCACTATGTTCGTTATTTCATCGATGATTAATTTATGTATTGGTAACGACTAACTATTTTTTTAATATAAATAATTGTGTAGGCTCCTTTTGTTGTTTGCCGTGTTTTTGACCGCCATAGAGGGATGGATCTGGACACTGAATTGTGGGCCTTATCTGATGTCGATGAAGGTGATGTTGTTGGTGACGACTCTGATGAGAAGGCGAATGAAGGAACTCAACAACTTAAGGGGGTTGGGACTAGTTATAGTGCTGATGCTAGTGCTGAGCAAGGTGATGCATTGCATTTCATGGCTACTCAAGCCACTTTTCAGGTAAAATATATTTCATTTTGATCTCTCGATATTTATTTTGTCATCGGCAACAATTTTACAATGCTTCACTTTCAATTAGACGTGAGTTTGAACAAAATTCCTCCATCATATGCGGGCCACAATATTAATGATTTTTATGTATGTATGACCAACTTTTAAATCATGGTCACACTATAGGTGGCTATTCAAAACCTTTATGTGTTGGTAGTTTTTGTGGTTGATGTTTGAATCCAAATAGCTCCATTGTGTTTGGATTATTTATGTTTTGCAGTTCCCAATTACAAAATTCTACATCCACAATTGTATCAAAAATTATGTTTAATTACGGAAGTCAATATTGGCAGTTAACCAGTTAATGTTGTTGATCCTCTTTGCAACTGAATTTTACAACTCAATTCATTCATGTGATGTTATTTTTTTTTTATTTTGATCAATTTTATTGAATGTATCACTCTGTACATGTTTTTTTCTTTCTTTTTTTCTTTTTTTTATGTAGAAATTAAGTGATGATCCTTAAAACATGTTAAAAATTAATACTAGTTAACATAGCTATTCTAACCTGATTCCTCAAAACATGTCCTTTTTATTTTTCTTTAAGTTTTTGTGGTATTAAATTCATTATTATTACTAATATAATCATCACTTTTTGACAAAGCATCTTTTCGTGTAATTTTATGGTGGAGAAAAGAAAGTGTTGTTGAAATATTGAATTTCTTTGTTTGATATTGATATTTATCAGGCGAGTTATGATGCATTTCTTGATTGTGTTGTGTCCCACGTGGAAGCTATGACGCCGGAGCAATTTAAGCTTGCAATTATGGACGAAGATTTTATTTAA
Protein sequence

>MS.gene040802.t1

MDLLPPGVGFVPSDELLVSYYLANKNNEEVQNFHGSDLIKELNMYQYDPFELHLARSFFFPHTREFYCFTAKSDELRRHCKTGFWMQMGAVEDITVGADDVLVGKKSLFFFYIGNSPQNAIKTSWTMYEYTLVNNPQAPFVVCRVFDRHRGMDLDTELWALSDVDEGDVVGDDSDEKANEGTQQLKGVGTSYSADASAEQGDALHFMATQATFQFPITKFYIHNCIKNYASYDAFLDCVVSHVEAMTPEQFKLAIMDEDFI