Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04092.t1 | XP_003590850.2 | 81.3 | 578 | 91 | 3 | 1 | 570 | 1 | 569 | 6.70E-263 | 916.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04092.t1 | Q9LUZ4 | 48.6 | 588 | 258 | 18 | 21 | 578 | 2 | 575 | 8.7e-134 | 478.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04092.t1 | G7I4W5 | 81.3 | 578 | 91 | 3 | 1 | 570 | 1 | 569 | 4.9e-263 | 916.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene04092.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04092.t1 | MTR_1g075890 | 80.623 | 578 | 95 | 2 | 1 | 570 | 1 | 569 | 0.0 | 905 |
| MS.gene04092.t1 | MTR_3g464280 | 66.899 | 574 | 109 | 7 | 1 | 574 | 1 | 493 | 0.0 | 697 |
| MS.gene04092.t1 | MTR_3g464260 | 68.315 | 546 | 73 | 7 | 37 | 579 | 29 | 477 | 0.0 | 682 |
| MS.gene04092.t1 | MTR_5g071070 | 54.103 | 658 | 214 | 16 | 1 | 578 | 1 | 650 | 0.0 | 612 |
| MS.gene04092.t1 | MTR_5g071070 | 54.340 | 576 | 177 | 15 | 81 | 578 | 1 | 568 | 0.0 | 528 |
| MS.gene04092.t1 | MTR_5g082830 | 47.200 | 625 | 238 | 15 | 28 | 578 | 25 | 631 | 1.53e-174 | 510 |
| MS.gene04092.t1 | MTR_7g092070 | 41.252 | 623 | 269 | 13 | 30 | 560 | 44 | 661 | 3.55e-139 | 421 |
| MS.gene04092.t1 | MTR_3g070880 | 42.226 | 566 | 212 | 14 | 31 | 578 | 8 | 476 | 3.14e-132 | 396 |
| MS.gene04092.t1 | MTR_3g464290 | 56.553 | 412 | 133 | 9 | 177 | 575 | 56 | 434 | 1.59e-126 | 380 |
| MS.gene04092.t1 | MTR_3g029590 | 39.200 | 625 | 279 | 14 | 30 | 556 | 42 | 663 | 3.87e-126 | 387 |
| MS.gene04092.t1 | MTR_3g464270 | 54.472 | 369 | 131 | 7 | 220 | 578 | 8 | 349 | 5.27e-116 | 349 |
| MS.gene04092.t1 | MTR_1g050438 | 40.426 | 470 | 225 | 11 | 32 | 458 | 14 | 471 | 1.73e-99 | 314 |
| MS.gene04092.t1 | MTR_0728s0020 | 40.426 | 470 | 225 | 11 | 32 | 458 | 30 | 487 | 4.69e-99 | 314 |
| MS.gene04092.t1 | MTR_3g435590 | 46.260 | 361 | 175 | 4 | 30 | 372 | 29 | 388 | 6.18e-98 | 306 |
| MS.gene04092.t1 | MTR_3g435680 | 43.860 | 285 | 121 | 8 | 118 | 372 | 26 | 301 | 2.97e-67 | 224 |
| MS.gene04092.t1 | MTR_8g102100 | 53.659 | 164 | 70 | 1 | 217 | 374 | 77 | 240 | 9.36e-55 | 190 |
| MS.gene04092.t1 | MTR_6g090380 | 59.848 | 132 | 44 | 1 | 192 | 323 | 83 | 205 | 4.57e-53 | 186 |
| MS.gene04092.t1 | MTR_3g103960 | 58.088 | 136 | 50 | 2 | 211 | 345 | 43 | 172 | 5.25e-51 | 177 |
| MS.gene04092.t1 | MTR_2g090605 | 60.360 | 111 | 44 | 0 | 221 | 331 | 77 | 187 | 3.53e-47 | 171 |
| MS.gene04092.t1 | MTR_4g078660 | 57.009 | 107 | 46 | 0 | 217 | 323 | 56 | 162 | 1.16e-44 | 161 |
| MS.gene04092.t1 | MTR_1g081420 | 83.333 | 48 | 8 | 0 | 492 | 539 | 181 | 228 | 1.72e-18 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04092.t1 | AT5G58620 | 49.735 | 565 | 241 | 16 | 21 | 556 | 2 | 552 | 4.97e-169 | 494 |
| MS.gene04092.t1 | AT2G40140 | 46.141 | 596 | 253 | 18 | 1 | 578 | 1 | 546 | 1.61e-156 | 461 |
| MS.gene04092.t1 | AT2G40140 | 46.141 | 596 | 253 | 18 | 1 | 578 | 1 | 546 | 1.61e-156 | 461 |
| MS.gene04092.t1 | AT3G55980 | 44.706 | 595 | 255 | 17 | 1 | 578 | 1 | 538 | 1.97e-152 | 450 |
| MS.gene04092.t1 | AT3G55980 | 44.706 | 595 | 255 | 17 | 1 | 578 | 68 | 605 | 1.46e-151 | 450 |
| MS.gene04092.t1 | AT5G12850 | 41.120 | 625 | 265 | 19 | 25 | 561 | 34 | 643 | 6.44e-138 | 417 |
| MS.gene04092.t1 | AT2G41900 | 47.109 | 467 | 195 | 10 | 25 | 454 | 40 | 491 | 6.60e-131 | 399 |
| MS.gene04092.t1 | AT4G29190 | 49.438 | 178 | 68 | 2 | 210 | 380 | 68 | 230 | 1.23e-53 | 186 |
| MS.gene04092.t1 | AT5G07500 | 61.983 | 121 | 46 | 0 | 203 | 323 | 34 | 154 | 2.13e-53 | 182 |
| MS.gene04092.t1 | AT2G25900 | 58.333 | 132 | 55 | 0 | 214 | 345 | 83 | 214 | 6.57e-52 | 181 |
| MS.gene04092.t1 | AT2G25900 | 58.333 | 132 | 55 | 0 | 214 | 345 | 83 | 214 | 6.57e-52 | 181 |
| MS.gene04092.t1 | AT2G19810 | 62.295 | 122 | 44 | 1 | 206 | 325 | 61 | 182 | 6.67e-52 | 182 |
| MS.gene04092.t1 | AT1G03790 | 52.229 | 157 | 67 | 3 | 186 | 338 | 51 | 203 | 9.04e-49 | 174 |
| MS.gene04092.t1 | AT5G44260 | 57.812 | 128 | 49 | 2 | 199 | 325 | 42 | 165 | 4.82e-46 | 167 |
Find 133 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCTTCATTGTGCTGTTTC+TGG | 0.098846 | 3.2:-53015254 | MS.gene04092:CDS |
| CTTATGGTTGCTGCCTTATT+TGG | 0.182687 | 3.2:-53015356 | MS.gene04092:CDS |
| GCCTTCTACCTTCTCTGGTT+GGG | 0.241460 | 3.2:-53014022 | MS.gene04092:CDS |
| TTGTCAAACTAGTTTGATTA+AGG | 0.245776 | 3.2:-53015567 | MS.gene04092:CDS |
| GGTGTGTTGTCTTATATTCT+TGG | 0.275343 | 3.2:-53015326 | MS.gene04092:CDS |
| TTGATTAAGGAAAATGAATA+TGG | 0.284568 | 3.2:-53015554 | MS.gene04092:CDS |
| CACACCCTTGCTTCCAAATA+AGG | 0.291991 | 3.2:+53015343 | None:intergenic |
| ATGAGGAAATCTTATTCTTT+CGG | 0.306520 | 3.2:-53013946 | MS.gene04092:CDS |
| TTCTCGGCATTTGAAGGGTT+GGG | 0.308891 | 3.2:+53014418 | None:intergenic |
| GAACGAAAACCAACAACTTT+GGG | 0.309040 | 3.2:-53014196 | MS.gene04092:CDS |
| TATATAGTACAGATGAATTT+AGG | 0.309340 | 3.2:-53014947 | MS.gene04092:CDS |
| CAAGGGCTTATTCTCATGAT+TGG | 0.318225 | 3.2:-53014896 | MS.gene04092:CDS |
| GAAAGCCATTACATCATCTT+TGG | 0.329332 | 3.2:+53015481 | None:intergenic |
| ACTCCTGCAAGCTTATTTGA+TGG | 0.329612 | 3.2:+53014288 | None:intergenic |
| CGAGTGTCCATTTGTTCACC+TGG | 0.330093 | 3.2:-53014871 | MS.gene04092:CDS |
| TTCTGCTACCTTGGTTGCCA+TGG | 0.342806 | 3.2:-53014046 | MS.gene04092:CDS |
| TTTGAAGTGGTAAGATTAGA+AGG | 0.343131 | 3.2:+53014168 | None:intergenic |
| CTTGTTGCTGCTCCACCGTT+TGG | 0.345377 | 3.2:+53015042 | None:intergenic |
| GTGTACCTTGCCCCGAGTTT+CGG | 0.361454 | 3.2:-53014803 | MS.gene04092:CDS |
| ATGGAGCCTTCTACCTTCTC+TGG | 0.362092 | 3.2:-53014027 | MS.gene04092:CDS |
| TCTCCATCAAATAAGCTTGC+AGG | 0.370221 | 3.2:-53014291 | MS.gene04092:CDS |
| ATCTGTTTCTCGGCATTTGA+AGG | 0.372092 | 3.2:+53014412 | None:intergenic |
| AGCAAAGAAGCAAACCCTTC+TGG | 0.372700 | 3.2:+53014668 | None:intergenic |
| CGAGCTTCCTTCTGCTACCT+TGG | 0.377977 | 3.2:-53014055 | MS.gene04092:CDS |
| CTAATAGTATCTTCGGTTCA+AGG | 0.385082 | 3.2:-53015121 | MS.gene04092:CDS |
| TTCACCTAGCTCTTCTTCAA+TGG | 0.396489 | 3.2:-53014571 | MS.gene04092:CDS |
| TGAAGAGAGGACACCTCTTA+TGG | 0.399251 | 3.2:-53015372 | MS.gene04092:CDS |
| CTTCCTTGCAAAGCCTTGTT+CGG | 0.403908 | 3.2:+53014703 | None:intergenic |
| GTTGAAGTGCTACTTCTGTT+TGG | 0.418213 | 3.2:+53013919 | None:intergenic |
| TTTCTCGGCATTTGAAGGGT+TGG | 0.421787 | 3.2:+53014417 | None:intergenic |
| TCAAAGTGAAGCCTTGTTCA+AGG | 0.431149 | 3.2:-53014914 | MS.gene04092:CDS |
| TCGATTAACATCGACACAAC+CGG | 0.437571 | 3.2:+53015301 | None:intergenic |
| AAGGATAACCCCAAAGTTGT+TGG | 0.439414 | 3.2:+53014187 | None:intergenic |
| AGAACGAAAACCAACAACTT+TGG | 0.439453 | 3.2:-53014197 | MS.gene04092:CDS |
| CCCCAACCAGAGAAGGTAGA+AGG | 0.441981 | 3.2:+53014021 | None:intergenic |
| CCTTCTACCTTCTCTGGTTG+GGG | 0.443126 | 3.2:-53014021 | MS.gene04092:CDS |
| CCATCATTAGCTTACACATA+AGG | 0.444911 | 3.2:+53014334 | None:intergenic |
| TTGTCTTATATTCTTGGAAC+CGG | 0.445771 | 3.2:-53015320 | MS.gene04092:CDS |
| TTCAGCCAAAGATGATGTAA+TGG | 0.447315 | 3.2:-53015486 | MS.gene04092:CDS |
| AAAACTGCACAAAATACTAT+AGG | 0.447954 | 3.2:-53014387 | MS.gene04092:CDS |
| GCAAACCCTTCTGGTGCATC+CGG | 0.454052 | 3.2:+53014677 | None:intergenic |
| CAGTTGATATGAACGGTAAC+CGG | 0.454347 | 3.2:-53015169 | MS.gene04092:CDS |
| GCACGACCCTTTCCGAAACT+CGG | 0.454722 | 3.2:+53014791 | None:intergenic |
| TAATCTTTCTTCTCAGTTCG+AGG | 0.455693 | 3.2:+53015008 | None:intergenic |
| AGCCTTCTACCTTCTCTGGT+TGG | 0.455905 | 3.2:-53014023 | MS.gene04092:CDS |
| TCGGCTTCTAATAGTATCTT+CGG | 0.460320 | 3.2:-53015128 | MS.gene04092:CDS |
| GCAGCATCATCGCATGCAGC+TGG | 0.462576 | 3.2:-53014480 | MS.gene04092:CDS |
| TGCTGCCTTATTTGGAAGCA+AGG | 0.473427 | 3.2:-53015348 | MS.gene04092:CDS |
| CACGACCCTTTCCGAAACTC+GGG | 0.473623 | 3.2:+53014792 | None:intergenic |
| TCTGGTTGGGGATCCCCTGA+TGG | 0.473856 | 3.2:-53014009 | MS.gene04092:CDS |
| ATTGAGTTCATCGCCGCGAA+TGG | 0.474643 | 3.2:+53013972 | None:intergenic |
| AATAAGGCAGCAACCATAAG+AGG | 0.478339 | 3.2:+53015359 | None:intergenic |
| CTTACCACTTCAAATATGAA+TGG | 0.481357 | 3.2:-53014159 | MS.gene04092:CDS |
| TACAACGGTCGAAGCTCCTC+TGG | 0.485266 | 3.2:+53014639 | None:intergenic |
| AGGCTTTGCAAGGAAGGAAC+CGG | 0.488722 | 3.2:-53014696 | MS.gene04092:CDS |
| CGACCGTTGTATGCTTCTAC+CGG | 0.490079 | 3.2:-53014627 | MS.gene04092:CDS |
| GAGGGTTGACTCAAAGGAAA+TGG | 0.493728 | 3.2:-53015399 | MS.gene04092:CDS |
| AGCCTTGTTCGGTATTGAGC+AGG | 0.501073 | 3.2:+53014714 | None:intergenic |
| AGGTGAAGGCAAAGCAGAAC+CGG | 0.502979 | 3.2:+53014608 | None:intergenic |
| TTCTGCGCTCAATAAAGCTC+TGG | 0.504121 | 3.2:+53014097 | None:intergenic |
| CGAACAAGGCTTTGCAAGGA+AGG | 0.512498 | 3.2:-53014702 | MS.gene04092:CDS |
| CCTTATGTGTAAGCTAATGA+TGG | 0.517096 | 3.2:-53014334 | MS.gene04092:CDS |
| CAAAGTGAAGCCTTGTTCAA+GGG | 0.517860 | 3.2:-53014913 | MS.gene04092:CDS |
| TCGAGTGTGCGGCTCAGATA+AGG | 0.525383 | 3.2:-53015282 | MS.gene04092:CDS |
| TCAGGTAGGGATGGATCAAT+CGG | 0.525749 | 3.2:+53014984 | None:intergenic |
| TAGGGTTATGGTATGGAAGG+AGG | 0.528404 | 3.2:-53015418 | MS.gene04092:CDS |
| GTCATGTGCAACGGTGCCAA+TGG | 0.528443 | 3.2:-53015596 | MS.gene04092:CDS |
| GTATCTTCGGTTCAAGGAAG+AGG | 0.528667 | 3.2:-53015115 | MS.gene04092:CDS |
| CTTGCCCCGAGTTTCGGAAA+GGG | 0.535224 | 3.2:-53014797 | MS.gene04092:CDS |
| TGGAAGGAGGGTTGACTCAA+AGG | 0.535765 | 3.2:-53015405 | MS.gene04092:CDS |
| AGGGTTATGGTATGGAAGGA+GGG | 0.536148 | 3.2:-53015417 | MS.gene04092:CDS |
| TCTGTTTCTCGGCATTTGAA+GGG | 0.538648 | 3.2:+53014413 | None:intergenic |
| CCTTGCCCCGAGTTTCGGAA+AGG | 0.539238 | 3.2:-53014798 | MS.gene04092:CDS |
| AAATTAGACTGGTCCATTCG+CGG | 0.540183 | 3.2:-53013985 | MS.gene04092:CDS |
| GAAATTAGGTTTCCAAACGG+TGG | 0.540685 | 3.2:-53015054 | MS.gene04092:CDS |
| GCTGCCTTATTTGGAAGCAA+GGG | 0.541989 | 3.2:-53015347 | MS.gene04092:CDS |
| TAAGAAATTAGGTTTCCAAA+CGG | 0.542543 | 3.2:-53015057 | MS.gene04092:CDS |
| ATGCAAGGCGTCGTGATCCG+AGG | 0.550851 | 3.2:-53014842 | MS.gene04092:CDS |
| CAGAAGTAGCACTTCAACAA+TGG | 0.554783 | 3.2:-53013914 | MS.gene04092:CDS |
| ATGTTGATGAGGTAGGGTTA+TGG | 0.554961 | 3.2:-53015430 | MS.gene04092:CDS |
| CCCCTGATGGAAAATTAGAC+TGG | 0.563187 | 3.2:-53013996 | MS.gene04092:CDS |
| GGTGAATTTGAATATGATGT+AGG | 0.568172 | 3.2:+53014588 | None:intergenic |
| GAATCCATTGAAGAAGAGCT+AGG | 0.570326 | 3.2:+53014567 | None:intergenic |
| TTGTTCTGCTGCTTCTTCTG+AGG | 0.570736 | 3.2:-53015231 | MS.gene04092:CDS |
| GATCATGATGTTGATGAGGT+AGG | 0.571630 | 3.2:-53015437 | MS.gene04092:CDS |
| ATCATGATGTTGATGAGGTA+GGG | 0.573336 | 3.2:-53015436 | MS.gene04092:CDS |
| GAAGAGGATACTACTAGAAG+AGG | 0.573450 | 3.2:-53015099 | MS.gene04092:CDS |
| CATGTGTAATGATATTTCCT+CGG | 0.575514 | 3.2:+53014825 | None:intergenic |
| GAGTGTCCATTTGTTCACCT+GGG | 0.577241 | 3.2:-53014870 | MS.gene04092:CDS |
| ATCCTGCTCAATACCGAACA+AGG | 0.577844 | 3.2:-53014716 | MS.gene04092:CDS |
| CTTCTTTGCTCACAAACCAG+AGG | 0.579205 | 3.2:-53014655 | MS.gene04092:CDS |
| AGGGGATCCCCAACCAGAGA+AGG | 0.582505 | 3.2:+53014014 | None:intergenic |
| TGATCCATTCATATTTGAAG+TGG | 0.582834 | 3.2:+53014155 | None:intergenic |
| CCTTTCCGAAACTCGGGGCA+AGG | 0.585775 | 3.2:+53014798 | None:intergenic |
| CAAGGTAGCAGAAGGAAGCT+CGG | 0.588994 | 3.2:+53014056 | None:intergenic |
| CAGGTGAACAAATGGACACT+CGG | 0.589724 | 3.2:+53014872 | None:intergenic |
| GTTAGTTCAGTTGATATGAA+CGG | 0.593158 | 3.2:-53015176 | MS.gene04092:CDS |
| ATACCGAACAAGGCTTTGCA+AGG | 0.594223 | 3.2:-53014706 | MS.gene04092:CDS |
| GCATGCGATGATGCTGCAGA+TGG | 0.598498 | 3.2:+53014486 | None:intergenic |
| AGGTAGGGTTATGGTATGGA+AGG | 0.598568 | 3.2:-53015421 | MS.gene04092:CDS |
| AGACAATCAAGTCGCTACAC+CGG | 0.604044 | 3.2:+53015150 | None:intergenic |
| GATGAGGTAGGGTTATGGTA+TGG | 0.604921 | 3.2:-53015425 | MS.gene04092:CDS |
| AAGGAACCGGATGCACCAGA+AGG | 0.605474 | 3.2:-53014683 | MS.gene04092:CDS |
| AGAAGGCTCCATGGCAACCA+AGG | 0.607516 | 3.2:+53014038 | None:intergenic |
| GCGATGAACTCAATAAAATG+AGG | 0.608002 | 3.2:-53013963 | MS.gene04092:CDS |
| GTGGAGCAGCAACAAGATGT+AGG | 0.608133 | 3.2:-53015035 | MS.gene04092:CDS |
| AACGAAAACCAACAACTTTG+GGG | 0.608292 | 3.2:-53014195 | MS.gene04092:CDS |
| TGATACAACTGCATTGATGT+CGG | 0.609284 | 3.2:+53014219 | None:intergenic |
| AGAGAAGGTAGAAGGCTCCA+TGG | 0.618438 | 3.2:+53014029 | None:intergenic |
| ATGGCAACCAAGGTAGCAGA+AGG | 0.618719 | 3.2:+53014048 | None:intergenic |
| GTGTAGCGACTTGATTGTCT+CGG | 0.619917 | 3.2:-53015147 | MS.gene04092:CDS |
| AGGAAATGGCATATGAAGAG+AGG | 0.622410 | 3.2:-53015385 | MS.gene04092:CDS |
| AGTGTCCATTTGTTCACCTG+GGG | 0.622949 | 3.2:-53014869 | MS.gene04092:CDS |
| GCAGATTGTATCAAAGAAGA+CGG | 0.625127 | 3.2:+53014528 | None:intergenic |
| GATGGAGTCAAAGGTGGTGT+CGG | 0.626172 | 3.2:+53014504 | None:intergenic |
| TGAGAATAAGCCCTTGAACA+AGG | 0.627841 | 3.2:+53014903 | None:intergenic |
| CTAATGATGGAAGAGAAGAC+AGG | 0.628766 | 3.2:-53014321 | MS.gene04092:CDS |
| AAAATCAAAGTCATGTGCAA+CGG | 0.635454 | 3.2:-53015605 | None:intergenic |
| CTGAATCTGAGTCTGCCACA+TGG | 0.636502 | 3.2:+53014455 | None:intergenic |
| TTTGAATATGATGTAGGTGA+AGG | 0.636602 | 3.2:+53014594 | None:intergenic |
| AGGAACCGGATGCACCAGAA+GGG | 0.647933 | 3.2:-53014682 | MS.gene04092:CDS |
| TTAATCAAACTAGTTTGACA+AGG | 0.649059 | 3.2:+53015569 | None:intergenic |
| GATGCTGCAGATGGAGTCAA+AGG | 0.650089 | 3.2:+53014495 | None:intergenic |
| AGATGATCATGATGTTGATG+AGG | 0.655942 | 3.2:-53015441 | MS.gene04092:CDS |
| ACGACCCTTTCCGAAACTCG+GGG | 0.660326 | 3.2:+53014793 | None:intergenic |
| AATGCATCAACTCTAAATTG+TGG | 0.663405 | 3.2:+53014132 | None:intergenic |
| ATACTACTAGAAGAGGTAGA+CGG | 0.679430 | 3.2:-53015092 | MS.gene04092:CDS |
| GTCGATGTTAATCGAGTGTG+CGG | 0.681504 | 3.2:-53015293 | MS.gene04092:CDS |
| GTGTCCATTTGTTCACCTGG+GGG | 0.681863 | 3.2:-53014868 | MS.gene04092:CDS |
| GCTGCAGATGGAGTCAAAGG+TGG | 0.704806 | 3.2:+53014498 | None:intergenic |
| CGCATGCAGCTGGAACCATG+TGG | 0.722861 | 3.2:-53014470 | MS.gene04092:CDS |
| GATGCTTGTGAATATGCACA+TGG | 0.753418 | 3.2:-53014759 | MS.gene04092:CDS |
| GAACCGGTAGAAGCATACAA+CGG | 0.780700 | 3.2:+53014624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATAGTACAGATGAATTT+AGG | - | chr3.2:53014522-53014541 | MS.gene04092:CDS | 20.0% |
| !! | TTGATTAAGGAAAATGAATA+TGG | - | chr3.2:53013915-53013934 | MS.gene04092:CDS | 20.0% |
| ! | AAAACTGCACAAAATACTAT+AGG | - | chr3.2:53015082-53015101 | MS.gene04092:CDS | 25.0% |
| ! | ATGAGGAAATCTTATTCTTT+CGG | - | chr3.2:53015523-53015542 | MS.gene04092:CDS | 25.0% |
| ! | TAAGAAATTAGGTTTCCAAA+CGG | - | chr3.2:53014412-53014431 | MS.gene04092:CDS | 25.0% |
| !! | ACTAACCTTAAAAACACTTT+TGG | - | chr3.2:53015211-53015230 | MS.gene04092:CDS | 25.0% |
| !! | CTAACCTTAAAAACACTTTT+GGG | - | chr3.2:53015212-53015231 | MS.gene04092:CDS | 25.0% |
| !! | GACGATTTTCTTAAGAAATT+AGG | - | chr3.2:53014401-53014420 | MS.gene04092:CDS | 25.0% |
| !! | TTAATCAAACTAGTTTGACA+AGG | + | chr3.2:53013903-53013922 | None:intergenic | 25.0% |
| !! | TTGTCAAACTAGTTTGATTA+AGG | - | chr3.2:53013902-53013921 | MS.gene04092:CDS | 25.0% |
| !!! | AAAGTGTTTTTAAGGTTAGT+AGG | + | chr3.2:53015211-53015230 | None:intergenic | 25.0% |
| !!! | ATTTTCAAGTTCGATACATT+CGG | + | chr3.2:53015113-53015132 | None:intergenic | 25.0% |
| AATGCATCAACTCTAAATTG+TGG | + | chr3.2:53015340-53015359 | None:intergenic | 30.0% | |
| CATGTGTAATGATATTTCCT+CGG | + | chr3.2:53014647-53014666 | None:intergenic | 30.0% | |
| CTTACCACTTCAAATATGAA+TGG | - | chr3.2:53015310-53015329 | MS.gene04092:CDS | 30.0% | |
| GGTGAATTTGAATATGATGT+AGG | + | chr3.2:53014884-53014903 | None:intergenic | 30.0% | |
| TGATCCATTCATATTTGAAG+TGG | + | chr3.2:53015317-53015336 | None:intergenic | 30.0% | |
| TTGTCTTATATTCTTGGAAC+CGG | - | chr3.2:53014149-53014168 | MS.gene04092:CDS | 30.0% | |
| TTTGAATATGATGTAGGTGA+AGG | + | chr3.2:53014878-53014897 | None:intergenic | 30.0% | |
| ! | GTTAGTTCAGTTGATATGAA+CGG | - | chr3.2:53014293-53014312 | MS.gene04092:CDS | 30.0% |
| ! | TATATCCCAGTTTTTATGTC+AGG | + | chr3.2:53014506-53014525 | None:intergenic | 30.0% |
| ! | TTTGAAGTGGTAAGATTAGA+AGG | + | chr3.2:53015304-53015323 | None:intergenic | 30.0% |
| !! | CACATGGTATTTTTGAATGT+TGG | - | chr3.2:53014726-53014745 | MS.gene04092:CDS | 30.0% |
| !!! | ATGACCCAAAAGTGTTTTTA+AGG | + | chr3.2:53015219-53015238 | None:intergenic | 30.0% |
| AACGAAAACCAACAACTTTG+GGG | - | chr3.2:53015274-53015293 | MS.gene04092:CDS | 35.0% | |
| AGAACGAAAACCAACAACTT+TGG | - | chr3.2:53015272-53015291 | MS.gene04092:CDS | 35.0% | |
| AGATGATCATGATGTTGATG+AGG | - | chr3.2:53014028-53014047 | MS.gene04092:CDS | 35.0% | |
| ATACTACTAGAAGAGGTAGA+CGG | - | chr3.2:53014377-53014396 | MS.gene04092:CDS | 35.0% | |
| CCTTATGTGTAAGCTAATGA+TGG | - | chr3.2:53015135-53015154 | MS.gene04092:CDS | 35.0% | |
| CTAATAGTATCTTCGGTTCA+AGG | - | chr3.2:53014348-53014367 | MS.gene04092:CDS | 35.0% | |
| GAAAGCCATTACATCATCTT+TGG | + | chr3.2:53013991-53014010 | None:intergenic | 35.0% | |
| GAACGAAAACCAACAACTTT+GGG | - | chr3.2:53015273-53015292 | MS.gene04092:CDS | 35.0% | |
| GCAGATTGTATCAAAGAAGA+CGG | + | chr3.2:53014944-53014963 | None:intergenic | 35.0% | |
| GCGATGAACTCAATAAAATG+AGG | - | chr3.2:53015506-53015525 | MS.gene04092:CDS | 35.0% | |
| GGTGTGTTGTCTTATATTCT+TGG | - | chr3.2:53014143-53014162 | MS.gene04092:CDS | 35.0% | |
| TAATCTTTCTTCTCAGTTCG+AGG | + | chr3.2:53014464-53014483 | None:intergenic | 35.0% | |
| TCGGCTTCTAATAGTATCTT+CGG | - | chr3.2:53014341-53014360 | MS.gene04092:CDS | 35.0% | |
| TTCAGCCAAAGATGATGTAA+TGG | - | chr3.2:53013983-53014002 | MS.gene04092:CDS | 35.0% | |
| ! | ACCAGTCTAATTTTCCATCA+GGG | + | chr3.2:53015477-53015496 | None:intergenic | 35.0% |
| ! | AGTTTGACAAGGTTTTCCAT+TGG | + | chr3.2:53013892-53013911 | None:intergenic | 35.0% |
| ! | ATCATGATGTTGATGAGGTA+GGG | - | chr3.2:53014033-53014052 | MS.gene04092:CDS | 35.0% |
| ! | CCATCATTAGCTTACACATA+AGG | + | chr3.2:53015138-53015157 | None:intergenic | 35.0% |
| ! | TGATACAACTGCATTGATGT+CGG | + | chr3.2:53015253-53015272 | None:intergenic | 35.0% |
| !! | GCAGTTTTCAATCTGTTTCT+CGG | + | chr3.2:53015070-53015089 | None:intergenic | 35.0% |
| AAGGATAACCCCAAAGTTGT+TGG | + | chr3.2:53015285-53015304 | None:intergenic | 40.0% | |
| AATAAGGCAGCAACCATAAG+AGG | + | chr3.2:53014113-53014132 | None:intergenic | 40.0% | |
| ACTCCTGCAAGCTTATTTGA+TGG | + | chr3.2:53015184-53015203 | None:intergenic | 40.0% | |
| AGGAAATGGCATATGAAGAG+AGG | - | chr3.2:53014084-53014103 | MS.gene04092:CDS | 40.0% | |
| CAAGGGCTTATTCTCATGAT+TGG | - | chr3.2:53014573-53014592 | MS.gene04092:CDS | 40.0% | |
| CAGAAGTAGCACTTCAACAA+TGG | - | chr3.2:53015555-53015574 | MS.gene04092:CDS | 40.0% | |
| CCCTACCTGACATAAAAACT+GGG | - | chr3.2:53014498-53014517 | MS.gene04092:CDS | 40.0% | |
| GAAATTAGGTTTCCAAACGG+TGG | - | chr3.2:53014415-53014434 | MS.gene04092:CDS | 40.0% | |
| GAAGAGGATACTACTAGAAG+AGG | - | chr3.2:53014370-53014389 | MS.gene04092:CDS | 40.0% | |
| GAATCCATTGAAGAAGAGCT+AGG | + | chr3.2:53014905-53014924 | None:intergenic | 40.0% | |
| GATGCTTGTGAATATGCACA+TGG | - | chr3.2:53014710-53014729 | MS.gene04092:CDS | 40.0% | |
| TCCCTACCTGACATAAAAAC+TGG | - | chr3.2:53014497-53014516 | MS.gene04092:CDS | 40.0% | |
| TCGATTAACATCGACACAAC+CGG | + | chr3.2:53014171-53014190 | None:intergenic | 40.0% | |
| TCTCCATCAAATAAGCTTGC+AGG | - | chr3.2:53015178-53015197 | MS.gene04092:CDS | 40.0% | |
| TGAGAATAAGCCCTTGAACA+AGG | + | chr3.2:53014569-53014588 | None:intergenic | 40.0% | |
| ! | AAATTAGACTGGTCCATTCG+CGG | - | chr3.2:53015484-53015503 | MS.gene04092:CDS | 40.0% |
| ! | ATCTGTTTCTCGGCATTTGA+AGG | + | chr3.2:53015060-53015079 | None:intergenic | 40.0% |
| ! | ATGTTGATGAGGTAGGGTTA+TGG | - | chr3.2:53014039-53014058 | MS.gene04092:CDS | 40.0% |
| ! | CAGTTGATATGAACGGTAAC+CGG | - | chr3.2:53014300-53014319 | MS.gene04092:CDS | 40.0% |
| ! | CCAGTCTAATTTTCCATCAG+GGG | + | chr3.2:53015476-53015495 | None:intergenic | 40.0% |
| ! | CCCAGTTTTTATGTCAGGTA+GGG | + | chr3.2:53014501-53014520 | None:intergenic | 40.0% |
| ! | CTAATGATGGAAGAGAAGAC+AGG | - | chr3.2:53015148-53015167 | MS.gene04092:CDS | 40.0% |
| ! | CTTATGGTTGCTGCCTTATT+TGG | - | chr3.2:53014113-53014132 | MS.gene04092:CDS | 40.0% |
| ! | GACCAGTCTAATTTTCCATC+AGG | + | chr3.2:53015478-53015497 | None:intergenic | 40.0% |
| ! | GATCATGATGTTGATGAGGT+AGG | - | chr3.2:53014032-53014051 | MS.gene04092:CDS | 40.0% |
| ! | GTTTTTATGTCAGGTAGGGA+TGG | + | chr3.2:53014497-53014516 | None:intergenic | 40.0% |
| ! | TCCCAGTTTTTATGTCAGGT+AGG | + | chr3.2:53014502-53014521 | None:intergenic | 40.0% |
| ! | TCTGTTTCTCGGCATTTGAA+GGG | + | chr3.2:53015059-53015078 | None:intergenic | 40.0% |
| ! | TTCACCTAGCTCTTCTTCAA+TGG | - | chr3.2:53014898-53014917 | MS.gene04092:CDS | 40.0% |
| !! | CAAAGTGAAGCCTTGTTCAA+GGG | - | chr3.2:53014556-53014575 | MS.gene04092:CDS | 40.0% |
| !! | GTTGAAGTGCTACTTCTGTT+TGG | + | chr3.2:53015553-53015572 | None:intergenic | 40.0% |
| !! | TCAAAGTGAAGCCTTGTTCA+AGG | - | chr3.2:53014555-53014574 | MS.gene04092:CDS | 40.0% |
| AGACAATCAAGTCGCTACAC+CGG | + | chr3.2:53014322-53014341 | None:intergenic | 45.0% | |
| AGCAAAGAAGCAAACCCTTC+TGG | + | chr3.2:53014804-53014823 | None:intergenic | 45.0% | |
| AGGGTTATGGTATGGAAGGA+GGG | - | chr3.2:53014052-53014071 | MS.gene04092:CDS | 45.0% | |
| AGGTAGGGTTATGGTATGGA+AGG | - | chr3.2:53014048-53014067 | MS.gene04092:CDS | 45.0% | |
| AGTGTCCATTTGTTCACCTG+GGG | - | chr3.2:53014600-53014619 | MS.gene04092:CDS | 45.0% | |
| ATACCGAACAAGGCTTTGCA+AGG | - | chr3.2:53014763-53014782 | MS.gene04092:CDS | 45.0% | |
| ATCCTGCTCAATACCGAACA+AGG | - | chr3.2:53014753-53014772 | MS.gene04092:CDS | 45.0% | |
| CACACCCTTGCTTCCAAATA+AGG | + | chr3.2:53014129-53014148 | None:intergenic | 45.0% | |
| CAGGTGAACAAATGGACACT+CGG | + | chr3.2:53014600-53014619 | None:intergenic | 45.0% | |
| CCCCTGATGGAAAATTAGAC+TGG | - | chr3.2:53015473-53015492 | MS.gene04092:CDS | 45.0% | |
| CTTCTTTGCTCACAAACCAG+AGG | - | chr3.2:53014814-53014833 | MS.gene04092:CDS | 45.0% | |
| GAGTGTCCATTTGTTCACCT+GGG | - | chr3.2:53014599-53014618 | MS.gene04092:CDS | 45.0% | |
| GATGAGGTAGGGTTATGGTA+TGG | - | chr3.2:53014044-53014063 | MS.gene04092:CDS | 45.0% | |
| GTATCTTCGGTTCAAGGAAG+AGG | - | chr3.2:53014354-53014373 | MS.gene04092:CDS | 45.0% | |
| GTCGATGTTAATCGAGTGTG+CGG | - | chr3.2:53014176-53014195 | MS.gene04092:CDS | 45.0% | |
| GTGTAGCGACTTGATTGTCT+CGG | - | chr3.2:53014322-53014341 | MS.gene04092:CDS | 45.0% | |
| TAGGGTTATGGTATGGAAGG+AGG | - | chr3.2:53014051-53014070 | MS.gene04092:CDS | 45.0% | |
| TCAGGTAGGGATGGATCAAT+CGG | + | chr3.2:53014488-53014507 | None:intergenic | 45.0% | |
| TGAAGAGAGGACACCTCTTA+TGG | - | chr3.2:53014097-53014116 | MS.gene04092:CDS | 45.0% | |
| TTCTCGGCATTTGAAGGGTT+GGG | + | chr3.2:53015054-53015073 | None:intergenic | 45.0% | |
| TTCTGCGCTCAATAAAGCTC+TGG | + | chr3.2:53015375-53015394 | None:intergenic | 45.0% | |
| TTTCTCGGCATTTGAAGGGT+TGG | + | chr3.2:53015055-53015074 | None:intergenic | 45.0% | |
| ! | GAACCGGTAGAAGCATACAA+CGG | + | chr3.2:53014848-53014867 | None:intergenic | 45.0% |
| ! | GAGGGTTGACTCAAAGGAAA+TGG | - | chr3.2:53014070-53014089 | MS.gene04092:CDS | 45.0% |
| ! | TTGTTCTGCTGCTTCTTCTG+AGG | - | chr3.2:53014238-53014257 | MS.gene04092:CDS | 45.0% |
| ! | TTTTCCCCCAGGTGAACAAA+TGG | + | chr3.2:53014608-53014627 | None:intergenic | 45.0% |
| !! | CTTCCTTGCAAAGCCTTGTT+CGG | + | chr3.2:53014769-53014788 | None:intergenic | 45.0% |
| !! | GCTCTTCATTGTGCTGTTTC+TGG | - | chr3.2:53014215-53014234 | MS.gene04092:CDS | 45.0% |
| !! | GCTGCCTTATTTGGAAGCAA+GGG | - | chr3.2:53014122-53014141 | MS.gene04092:CDS | 45.0% |
| !! | TGCTGCCTTATTTGGAAGCA+AGG | - | chr3.2:53014121-53014140 | MS.gene04092:CDS | 45.0% |
| AGAGAAGGTAGAAGGCTCCA+TGG | + | chr3.2:53015443-53015462 | None:intergenic | 50.0% | |
| AGCCTTCTACCTTCTCTGGT+TGG | - | chr3.2:53015446-53015465 | MS.gene04092:CDS | 50.0% | |
| AGGCTTTGCAAGGAAGGAAC+CGG | - | chr3.2:53014773-53014792 | MS.gene04092:CDS | 50.0% | |
| AGGTGAAGGCAAAGCAGAAC+CGG | + | chr3.2:53014864-53014883 | None:intergenic | 50.0% | |
| ATGGAGCCTTCTACCTTCTC+TGG | - | chr3.2:53015442-53015461 | MS.gene04092:CDS | 50.0% | |
| ATGGCAACCAAGGTAGCAGA+AGG | + | chr3.2:53015424-53015443 | None:intergenic | 50.0% | |
| ATTGAGTTCATCGCCGCGAA+TGG | + | chr3.2:53015500-53015519 | None:intergenic | 50.0% | |
| CAAGGTAGCAGAAGGAAGCT+CGG | + | chr3.2:53015416-53015435 | None:intergenic | 50.0% | |
| CCTTCTACCTTCTCTGGTTG+GGG | - | chr3.2:53015448-53015467 | MS.gene04092:CDS | 50.0% | |
| CGAACAAGGCTTTGCAAGGA+AGG | - | chr3.2:53014767-53014786 | MS.gene04092:CDS | 50.0% | |
| CGACCGTTGTATGCTTCTAC+CGG | - | chr3.2:53014842-53014861 | MS.gene04092:CDS | 50.0% | |
| CGAGTGTCCATTTGTTCACC+TGG | - | chr3.2:53014598-53014617 | MS.gene04092:CDS | 50.0% | |
| CTGAATCTGAGTCTGCCACA+TGG | + | chr3.2:53015017-53015036 | None:intergenic | 50.0% | |
| GATGCTGCAGATGGAGTCAA+AGG | + | chr3.2:53014977-53014996 | None:intergenic | 50.0% | |
| GATGGAGTCAAAGGTGGTGT+CGG | + | chr3.2:53014968-53014987 | None:intergenic | 50.0% | |
| GCCTTCTACCTTCTCTGGTT+GGG | - | chr3.2:53015447-53015466 | MS.gene04092:CDS | 50.0% | |
| GTGGAGCAGCAACAAGATGT+AGG | - | chr3.2:53014434-53014453 | MS.gene04092:CDS | 50.0% | |
| GTGTCCATTTGTTCACCTGG+GGG | - | chr3.2:53014601-53014620 | MS.gene04092:CDS | 50.0% | |
| TTCACCTGGGGGAAAATGCA+AGG | - | chr3.2:53014612-53014631 | MS.gene04092:CDS | 50.0% | |
| TTCTGCTACCTTGGTTGCCA+TGG | - | chr3.2:53015423-53015442 | MS.gene04092:CDS | 50.0% | |
| ! | TGGAAGGAGGGTTGACTCAA+AGG | - | chr3.2:53014064-53014083 | MS.gene04092:CDS | 50.0% |
| !! | AGCCTTGTTCGGTATTGAGC+AGG | + | chr3.2:53014758-53014777 | None:intergenic | 50.0% |
| AAGGAACCGGATGCACCAGA+AGG | - | chr3.2:53014786-53014805 | MS.gene04092:CDS | 55.0% | |
| ACGACCCTTTCCGAAACTCG+GGG | + | chr3.2:53014679-53014698 | None:intergenic | 55.0% | |
| AGAAGGCTCCATGGCAACCA+AGG | + | chr3.2:53015434-53015453 | None:intergenic | 55.0% | |
| AGGAACCGGATGCACCAGAA+GGG | - | chr3.2:53014787-53014806 | MS.gene04092:CDS | 55.0% | |
| CACGACCCTTTCCGAAACTC+GGG | + | chr3.2:53014680-53014699 | None:intergenic | 55.0% | |
| CCCCAACCAGAGAAGGTAGA+AGG | + | chr3.2:53015451-53015470 | None:intergenic | 55.0% | |
| CGAGCTTCCTTCTGCTACCT+TGG | - | chr3.2:53015414-53015433 | MS.gene04092:CDS | 55.0% | |
| CTTGCCCCGAGTTTCGGAAA+GGG | - | chr3.2:53014672-53014691 | MS.gene04092:CDS | 55.0% | |
| CTTGTTGCTGCTCCACCGTT+TGG | + | chr3.2:53014430-53014449 | None:intergenic | 55.0% | |
| GCAAACCCTTCTGGTGCATC+CGG | + | chr3.2:53014795-53014814 | None:intergenic | 55.0% | |
| GCACGACCCTTTCCGAAACT+CGG | + | chr3.2:53014681-53014700 | None:intergenic | 55.0% | |
| GCATGCGATGATGCTGCAGA+TGG | + | chr3.2:53014986-53015005 | None:intergenic | 55.0% | |
| GCTGCAGATGGAGTCAAAGG+TGG | + | chr3.2:53014974-53014993 | None:intergenic | 55.0% | |
| GTGTACCTTGCCCCGAGTTT+CGG | - | chr3.2:53014666-53014685 | MS.gene04092:CDS | 55.0% | |
| TACAACGGTCGAAGCTCCTC+TGG | + | chr3.2:53014833-53014852 | None:intergenic | 55.0% | |
| TCGAGTGTGCGGCTCAGATA+AGG | - | chr3.2:53014187-53014206 | MS.gene04092:CDS | 55.0% | |
| AGGGGATCCCCAACCAGAGA+AGG | + | chr3.2:53015458-53015477 | None:intergenic | 60.0% | |
| CCTTGCCCCGAGTTTCGGAA+AGG | - | chr3.2:53014671-53014690 | MS.gene04092:CDS | 60.0% | |
| CCTTTCCGAAACTCGGGGCA+AGG | + | chr3.2:53014674-53014693 | None:intergenic | 60.0% | |
| CGCATGCAGCTGGAACCATG+TGG | - | chr3.2:53014999-53015018 | MS.gene04092:CDS | 60.0% | |
| GCAGCATCATCGCATGCAGC+TGG | - | chr3.2:53014989-53015008 | MS.gene04092:CDS | 60.0% | |
| TCTGGTTGGGGATCCCCTGA+TGG | - | chr3.2:53015460-53015479 | MS.gene04092:CDS | 60.0% | |
| ! | GACGCCTTGCATTTTCCCCC+AGG | + | chr3.2:53014619-53014638 | None:intergenic | 60.0% |
| !! | ATGCAAGGCGTCGTGATCCG+AGG | - | chr3.2:53014627-53014646 | MS.gene04092:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 53013876 | 53015615 | 53013876 | ID=MS.gene04092 |
| chr3.2 | mRNA | 53013876 | 53015615 | 53013876 | ID=MS.gene04092.t1;Parent=MS.gene04092 |
| chr3.2 | exon | 53013876 | 53015615 | 53013876 | ID=MS.gene04092.t1.exon1;Parent=MS.gene04092.t1 |
| chr3.2 | CDS | 53013876 | 53015615 | 53013876 | ID=cds.MS.gene04092.t1;Parent=MS.gene04092.t1 |
| Gene Sequence |
| Protein sequence |
