Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040941.t1 | AFK46965.1 | 98.3 | 230 | 4 | 0 | 1 | 230 | 1 | 230 | 5.70E-122 | 447.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040941.t1 | Q8VZ42 | 61.9 | 239 | 81 | 3 | 1 | 238 | 1 | 230 | 1.7e-82 | 307.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040941.t1 | I3T373 | 98.3 | 230 | 4 | 0 | 1 | 230 | 1 | 230 | 4.1e-122 | 447.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene040941.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040941.t1 | MTR_0100s0160 | 97.902 | 286 | 6 | 0 | 1 | 286 | 1 | 286 | 0.0 | 584 |
| MS.gene040941.t1 | MTR_7g091810 | 65.157 | 287 | 79 | 2 | 1 | 285 | 1 | 268 | 6.17e-129 | 367 |
| MS.gene040941.t1 | MTR_6g008210 | 49.640 | 139 | 63 | 2 | 2 | 137 | 5 | 139 | 1.81e-38 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040941.t1 | AT2G41835 | 62.718 | 287 | 97 | 3 | 1 | 286 | 1 | 278 | 4.35e-128 | 365 |
| MS.gene040941.t1 | AT3G57480 | 53.310 | 287 | 96 | 2 | 1 | 287 | 1 | 249 | 1.37e-106 | 310 |
| MS.gene040941.t1 | AT3G57480 | 53.310 | 287 | 96 | 2 | 1 | 287 | 1 | 249 | 1.37e-106 | 310 |
| MS.gene040941.t1 | AT3G28210 | 41.622 | 185 | 78 | 4 | 2 | 183 | 5 | 162 | 2.39e-38 | 134 |
| MS.gene040941.t1 | AT3G28210 | 41.622 | 185 | 78 | 4 | 2 | 183 | 5 | 162 | 2.39e-38 | 134 |
| MS.gene040941.t1 | AT5G48205 | 40.260 | 154 | 33 | 4 | 1 | 151 | 1 | 98 | 2.84e-25 | 99.8 |
Find 0 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTCATTAGATTCATAAAA+GGG | + | 6949:184027-184046 | None:intergenic | 15.0% |
| !!! | AATCATATAAGCTATTTTTT+GGG | + | 6949:182848-182867 | None:intergenic | 15.0% |
| !!! | ACATTGTATTTTTATTTTTG+AGG | - | 6949:183490-183509 | MS.gene040941:intron | 15.0% |
| !!! | TAATCATATAAGCTATTTTT+TGG | + | 6949:182849-182868 | None:intergenic | 15.0% |
| !!! | TGATGATATTTTTATTTTGT+GGG | + | 6949:183221-183240 | None:intergenic | 15.0% |
| !!! | TTGATGATATTTTTATTTTG+TGG | + | 6949:183222-183241 | None:intergenic | 15.0% |
| !! | AAATTATTAAGAATGCATAG+TGG | + | 6949:182669-182688 | None:intergenic | 20.0% |
| !! | AAGAAAAAGAAAAGAGAAAT+TGG | + | 6949:182601-182620 | None:intergenic | 20.0% |
| !! | AATTATTAAGAATGCATAGT+GGG | + | 6949:182668-182687 | None:intergenic | 20.0% |
| !! | ATTGAAGTTTATCCTAATAT+AGG | + | 6949:182881-182900 | None:intergenic | 20.0% |
| !! | CAAATGGTTTATCAAAATTA+CGG | - | 6949:183355-183374 | MS.gene040941:intron | 20.0% |
| !! | CTATTCATTAGATTCATAAA+AGG | + | 6949:184028-184047 | None:intergenic | 20.0% |
| !! | TAACTTTCTCATAATTTGAT+GGG | + | 6949:183850-183869 | None:intergenic | 20.0% |
| !!! | AAATTTGTTTCCACTTTTTT+AGG | + | 6949:184004-184023 | None:intergenic | 20.0% |
| !!! | AGTTTTATTATAGCCTTTAT+AGG | - | 6949:183104-183123 | MS.gene040941:CDS | 20.0% |
| !!! | ATCATATAAGCTATTTTTTG+GGG | + | 6949:182847-182866 | None:intergenic | 20.0% |
| !!! | ATCTAATTGAAAAGTACTTT+TGG | - | 6949:182698-182717 | MS.gene040941:CDS | 20.0% |
| !!! | CTTTATTTAGATTTTATCTC+AGG | - | 6949:182785-182804 | MS.gene040941:CDS | 20.0% |
| !!! | CTTTTATGAATCTAATGAAT+AGG | - | 6949:184026-184045 | MS.gene040941:intron | 20.0% |
| ! | AAACTATCATGTGAACAAAT+TGG | - | 6949:183240-183259 | MS.gene040941:intron | 25.0% |
| ! | AATGAATAGGAGTAGAAAAA+AGG | - | 6949:184039-184058 | MS.gene040941:intron | 25.0% |
| ! | ACCAAAAATGAAAATCGTTT+AGG | + | 6949:183460-183479 | None:intergenic | 25.0% |
| ! | GTAACTTTCTCATAATTTGA+TGG | + | 6949:183851-183870 | None:intergenic | 25.0% |
| ! | TAATTAATACAAACTAGCGA+AGG | - | 6949:183044-183063 | MS.gene040941:CDS | 25.0% |
| ! | TATTATAGCCTTTATAGGAT+AGG | - | 6949:183109-183128 | MS.gene040941:CDS | 25.0% |
| ! | TTAATTAAACAGAAACAACG+TGG | + | 6949:182642-182661 | None:intergenic | 25.0% |
| ! | TTATTCGATAAGCTGTTATA+GGG | + | 6949:183019-183038 | None:intergenic | 25.0% |
| ! | TTTATTCGATAAGCTGTTAT+AGG | + | 6949:183020-183039 | None:intergenic | 25.0% |
| !! | CTAGATTTTATTAGGGTTTT+GGG | - | 6949:182722-182741 | MS.gene040941:CDS | 25.0% |
| !! | TCCTAAACGATTTTCATTTT+TGG | - | 6949:183456-183475 | MS.gene040941:intron | 25.0% |
| !! | TTTTGGCCTAGATTTTATTA+GGG | - | 6949:182715-182734 | MS.gene040941:CDS | 25.0% |
| !!! | ATTTTGTTTGATGACATTCT+TGG | - | 6949:183562-183581 | MS.gene040941:intron | 25.0% |
| !!! | TATAACTTGTTGCTTTGAAT+TGG | - | 6949:182940-182959 | MS.gene040941:CDS | 25.0% |
| !!! | TTTTGTTTGATGACATTCTT+GGG | - | 6949:183563-183582 | MS.gene040941:intron | 25.0% |
| AAATCGAAACTACCCTTAAA+TGG | + | 6949:182758-182777 | None:intergenic | 30.0% | |
| ATTACGGTATTGCAGAAAAT+AGG | - | 6949:183371-183390 | MS.gene040941:intron | 30.0% | |
| CAAAAAGTTCATGAAAGAAG+TGG | - | 6949:184304-184323 | MS.gene040941:intron | 30.0% | |
| CCAAAACCCTAATAAAATCT+AGG | + | 6949:182724-182743 | None:intergenic | 30.0% | |
| CCAAACAATACACCTAAAAT+GGG | + | 6949:183676-183695 | None:intergenic | 30.0% | |
| CTCAAACACAATTAAATGCA+AGG | - | 6949:183919-183938 | MS.gene040941:intron | 30.0% | |
| TATCATGTGAACAAATTGGA+AGG | - | 6949:183244-183263 | MS.gene040941:intron | 30.0% | |
| TCCAAACAATACACCTAAAA+TGG | + | 6949:183677-183696 | None:intergenic | 30.0% | |
| TTAAGTGCTTAGCCTATATT+AGG | - | 6949:182866-182885 | MS.gene040941:CDS | 30.0% | |
| TTACGGTATTGCAGAAAATA+GGG | - | 6949:183372-183391 | MS.gene040941:intron | 30.0% | |
| TTATAGCTTCTCAAAAGTCA+CGG | + | 6949:182925-182944 | None:intergenic | 30.0% | |
| ! | CCTAGATTTTATTAGGGTTT+TGG | - | 6949:182721-182740 | MS.gene040941:CDS | 30.0% |
| ! | CTTTTGGCCTAGATTTTATT+AGG | - | 6949:182714-182733 | MS.gene040941:CDS | 30.0% |
| ! | TGCATGAACTTTAGAAGTTA+AGG | + | 6949:183520-183539 | None:intergenic | 30.0% |
| ! | TTGAACAAACCTCTATTTTC+TGG | + | 6949:183293-183312 | None:intergenic | 30.0% |
| !!! | TTATTAGGGTTTTGGGTTTA+CGG | - | 6949:182729-182748 | MS.gene040941:CDS | 30.0% |
| AATAAACGCGAAGCTTCAAT+TGG | + | 6949:183177-183196 | None:intergenic | 35.0% | |
| ACCGATGTTTGATATAACTC+CGG | + | 6949:183703-183722 | None:intergenic | 35.0% | |
| ACCGGAGTTATATCAAACAT+CGG | - | 6949:183699-183718 | MS.gene040941:intron | 35.0% | |
| AGCAAGATCCAAACATAACA+TGG | - | 6949:183798-183817 | MS.gene040941:intron | 35.0% | |
| ATCGATTAAAGTAGTGATCG+AGG | + | 6949:184278-184297 | None:intergenic | 35.0% | |
| CAAAAAACAAACAGGCTCTT+AGG | - | 6949:182987-183006 | MS.gene040941:CDS | 35.0% | |
| CAAACCTATGCTTCAAACAA+TGG | + | 6949:183958-183977 | None:intergenic | 35.0% | |
| CTACAGCTACAATGCTATAA+CGG | - | 6949:183324-183343 | MS.gene040941:intron | 35.0% | |
| GCAAGATCCAAACATAACAT+GGG | - | 6949:183799-183818 | MS.gene040941:intron | 35.0% | |
| GGTTTACGGTATCCATTTAA+GGG | - | 6949:182743-182762 | MS.gene040941:CDS | 35.0% | |
| GTGAATCCTTTACTACACTT+AGG | + | 6949:184367-184386 | None:intergenic | 35.0% | |
| TAGCCTTTATAGGATAGGAT+AGG | - | 6949:183114-183133 | MS.gene040941:CDS | 35.0% | |
| TCTCCTATCCTATCCTATAA+AGG | + | 6949:183120-183139 | None:intergenic | 35.0% | |
| TGATTCTCCCATGTTATGTT+TGG | + | 6949:183809-183828 | None:intergenic | 35.0% | |
| TGGTGAAATTAACCAAGCAT+GGG | - | 6949:184174-184193 | MS.gene040941:intron | 35.0% | |
| TGTAGTCCATTTAGATGAAG+AGG | + | 6949:184092-184111 | None:intergenic | 35.0% | |
| TTTCCTAGATCTGGAAATTC+TGG | + | 6949:182500-182519 | None:intergenic | 35.0% | |
| ! | CAATACACCTAAAATGGGTT+TGG | + | 6949:183671-183690 | None:intergenic | 35.0% |
| ! | CATTGTTTGAAGCATAGGTT+TGG | - | 6949:183956-183975 | MS.gene040941:intron | 35.0% |
| ! | GTATAAGCCAAACCCATTTT+AGG | - | 6949:183661-183680 | MS.gene040941:intron | 35.0% |
| ! | TAGACCATTGTTTGAAGCAT+AGG | - | 6949:183951-183970 | MS.gene040941:intron | 35.0% |
| !! | CCCATTTTAGGTGTATTGTT+TGG | - | 6949:183673-183692 | MS.gene040941:intron | 35.0% |
| !!! | AGCCTGTTTGTTTTTTGAGT+GGG | + | 6949:182984-183003 | None:intergenic | 35.0% |
| AACTAGCGAAGGATGAGAAT+TGG | - | 6949:183055-183074 | MS.gene040941:CDS | 40.0% | |
| ACTCCAGAATTTCCAGATCT+AGG | - | 6949:182494-182513 | MS.gene040941:CDS | 40.0% | |
| AGTAGTGATCGAGGAAAACT+TGG | + | 6949:184269-184288 | None:intergenic | 40.0% | |
| AGTCCATTTAGATGAAGAGG+AGG | + | 6949:184089-184108 | None:intergenic | 40.0% | |
| ATACCACTTCCAATGCAAGT+TGG | - | 6949:182899-182918 | MS.gene040941:CDS | 40.0% | |
| ATATGTCCACTTTGCGCTAA+AGG | - | 6949:183758-183777 | MS.gene040941:intron | 40.0% | |
| ATGTCCAGGACCTAAAAAAG+TGG | - | 6949:183991-184010 | MS.gene040941:intron | 40.0% | |
| CAACCTCCTCTTCATCTAAA+TGG | - | 6949:184083-184102 | MS.gene040941:intron | 40.0% | |
| CACCCACTCAAAAAACAAAC+AGG | - | 6949:182979-182998 | MS.gene040941:CDS | 40.0% | |
| CGAAGCTCGAATATTAGAAG+CGG | + | 6949:184128-184147 | None:intergenic | 40.0% | |
| CGCTTCTAATATTCGAGCTT+CGG | - | 6949:184126-184145 | MS.gene040941:intron | 40.0% | |
| CGTGGACACATAAAAGTTGA+AGG | + | 6949:182624-182643 | None:intergenic | 40.0% | |
| GAAATCAACGAGCTTGCAAT+CGG | + | 6949:182537-182556 | None:intergenic | 40.0% | |
| GAAATTGGTTAGTACGAACC+TGG | + | 6949:182586-182605 | None:intergenic | 40.0% | |
| GGGTTTACGGTATCCATTTA+AGG | - | 6949:182742-182761 | MS.gene040941:CDS | 40.0% | |
| GGTGAAATTAACCAAGCATG+GGG | - | 6949:184175-184194 | MS.gene040941:intron | 40.0% | |
| GTGAAATTAACCAAGCATGG+GGG | - | 6949:184176-184195 | MS.gene040941:intron | 40.0% | |
| GTGGTGAAATTAACCAAGCA+TGG | - | 6949:184173-184192 | MS.gene040941:intron | 40.0% | |
| GTTATGTTTGGATCTTGCTC+AGG | + | 6949:183797-183816 | None:intergenic | 40.0% | |
| TATAACGGTGCAGAGTCAAA+TGG | - | 6949:183339-183358 | MS.gene040941:intron | 40.0% | |
| TGGTAACATTCGCAACAGAT+CGG | + | 6949:184333-184352 | None:intergenic | 40.0% | |
| ! | AGGTGTATTGTTTGGAACAC+CGG | - | 6949:183681-183700 | MS.gene040941:intron | 40.0% |
| ! | ATAATTTGATGGGTCGCAGT+CGG | + | 6949:183840-183859 | None:intergenic | 40.0% |
| ! | TCTTGGGAATTTGCGTTTTG+TGG | - | 6949:183579-183598 | MS.gene040941:intron | 40.0% |
| ! | TGGGTTTGGCTTATACAGTT+AGG | + | 6949:183657-183676 | None:intergenic | 40.0% |
| !!! | GAGCCTGTTTGTTTTTTGAG+TGG | + | 6949:182985-183004 | None:intergenic | 40.0% |
| !!! | GTTTCCACTTTTTTAGGTCC+TGG | + | 6949:183998-184017 | None:intergenic | 40.0% |
| AAAACTTGGCACCGCATTGA+GGG | + | 6949:184255-184274 | None:intergenic | 45.0% | |
| ACACTTAGGACACGCATCAA+TGG | + | 6949:184353-184372 | None:intergenic | 45.0% | |
| AGAGGAGGTTGAACTTGAGT+TGG | + | 6949:184074-184093 | None:intergenic | 45.0% | |
| AGGAGAGTGATGGTTATGAG+TGG | - | 6949:183134-183153 | MS.gene040941:CDS | 45.0% | |
| AGGATAGGATAGGAGAGTGA+TGG | - | 6949:183124-183143 | MS.gene040941:CDS | 45.0% | |
| ATCGCGGATCCAGAAAATAG+AGG | - | 6949:183281-183300 | MS.gene040941:intron | 45.0% | |
| GATGGTTATGAGTGGGAACT+GGG | - | 6949:183142-183161 | MS.gene040941:CDS | 45.0% | |
| GCGTGTCCTAAGTGTAGTAA+AGG | - | 6949:184358-184377 | MS.gene040941:CDS | 45.0% | |
| GCTGGAATGTCAAAGTTGAG+TGG | - | 6949:184154-184173 | MS.gene040941:intron | 45.0% | |
| GGAATCAGTGTTGTCAATCG+CGG | - | 6949:183265-183284 | MS.gene040941:intron | 45.0% | |
| GGAGAGTGATGGTTATGAGT+GGG | - | 6949:183135-183154 | MS.gene040941:CDS | 45.0% | |
| TAATATTCGAGCTTCGGCTG+AGG | - | 6949:184132-184151 | MS.gene040941:intron | 45.0% | |
| TGATGGTTATGAGTGGGAAC+TGG | - | 6949:183141-183160 | MS.gene040941:CDS | 45.0% | |
| TTGGATCTTGCTCAGGAACT+AGG | + | 6949:183790-183809 | None:intergenic | 45.0% | |
| ! | AATGGTGGGAGAAAGAGCAA+CGG | - | 6949:184208-184227 | MS.gene040941:intron | 45.0% |
| ! | GAGCAGTGTTTTCCTAGATC+TGG | + | 6949:182509-182528 | None:intergenic | 45.0% |
| AACATGCTCCACAAGGGACA+CGG | + | 6949:184395-184414 | None:intergenic | 50.0% | |
| AAGTCACGGCCAACTTGCAT+TGG | + | 6949:182911-182930 | None:intergenic | 50.0% | |
| ATCTCTTTGCATCCAGCTGC+AGG | + | 6949:183890-183909 | None:intergenic | 50.0% | |
| CATGTTGAGAGGGAACATGG+TGG | - | 6949:184409-184428 | MS.gene040941:CDS | 50.0% | |
| CCTCTCAACATGCTCCACAA+GGG | + | 6949:184401-184420 | None:intergenic | 50.0% | |
| CCTTGTGGAGCATGTTGAGA+GGG | - | 6949:184399-184418 | MS.gene040941:CDS | 50.0% | |
| GAAAACTTGGCACCGCATTG+AGG | + | 6949:184256-184275 | None:intergenic | 50.0% | |
| GAGCATGTTGAGAGGGAACA+TGG | - | 6949:184406-184425 | MS.gene040941:CDS | 50.0% | |
| GATCTTGCTCAGGAACTAGG+CGG | + | 6949:183787-183806 | None:intergenic | 50.0% | |
| GGTCCTGATCACAAATGTCC+AGG | - | 6949:183977-183996 | MS.gene040941:intron | 50.0% | |
| GGTCCTGGACATTTGTGATC+AGG | + | 6949:183983-184002 | None:intergenic | 50.0% | |
| TAGCAGCGATCGCAAGTGAA+AGG | + | 6949:182563-182582 | None:intergenic | 50.0% | |
| TCTCTTTGCATCCAGCTGCA+GGG | + | 6949:183889-183908 | None:intergenic | 50.0% | |
| !! | AAGAAAAAGTGCCCTGCAGC+TGG | - | 6949:183875-183894 | MS.gene040941:intron | 50.0% |
| ACATGCTCCACAAGGGACAC+GGG | + | 6949:184394-184413 | None:intergenic | 55.0% | |
| CCCTCTCAACATGCTCCACA+AGG | + | 6949:184402-184421 | None:intergenic | 55.0% | |
| CCCTTGTGGAGCATGTTGAG+AGG | - | 6949:184398-184417 | MS.gene040941:CDS | 55.0% | |
| CGGACTCCTTTAGCGCAAAG+TGG | + | 6949:183767-183786 | None:intergenic | 55.0% | |
| CGGCCAACTTGCATTGGAAG+TGG | + | 6949:182905-182924 | None:intergenic | 55.0% | |
| GCATGGGGGACATCAAGCAA+TGG | - | 6949:184190-184209 | MS.gene040941:intron | 55.0% | |
| GGGGGACATCAAGCAATGGT+GGG | - | 6949:184194-184213 | MS.gene040941:intron | 55.0% | |
| GTGGAGCAATGCCCTCAATG+CGG | - | 6949:184241-184260 | MS.gene040941:intron | 55.0% | |
| TGCTTGATGTCCCCCATGCT+TGG | + | 6949:184189-184208 | None:intergenic | 55.0% | |
| TGGGGGACATCAAGCAATGG+TGG | - | 6949:184193-184212 | MS.gene040941:intron | 55.0% | |
| ATTCGAGCTTCGGCTGAGGC+TGG | - | 6949:184136-184155 | MS.gene040941:intron | 60.0% | |
| CACAGATCCCGTGTCCCTTG+TGG | - | 6949:184384-184403 | MS.gene040941:CDS | 60.0% | |
| CACTTGCGATCGCTGCTACC+AGG | - | 6949:182565-182584 | MS.gene040941:CDS | 60.0% | |
| GAGCAACGGCAGTGGTCAAG+TGG | - | 6949:184222-184241 | MS.gene040941:intron | 60.0% | |
| GGGAGAAAGAGCAACGGCAG+TGG | - | 6949:184214-184233 | MS.gene040941:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6949 | gene | 182488 | 184447 | 182488 | ID=MS.gene040941 |
| 6949 | mRNA | 182488 | 184447 | 182488 | ID=MS.gene040941.t1;Parent=MS.gene040941 |
| 6949 | exon | 184349 | 184447 | 184349 | ID=MS.gene040941.t1.exon1;Parent=MS.gene040941.t1 |
| 6949 | CDS | 184349 | 184447 | 184349 | ID=cds.MS.gene040941.t1;Parent=MS.gene040941.t1 |
| 6949 | exon | 182488 | 183252 | 182488 | ID=MS.gene040941.t1.exon2;Parent=MS.gene040941.t1 |
| 6949 | CDS | 182488 | 183252 | 182488 | ID=cds.MS.gene040941.t1;Parent=MS.gene040941.t1 |
| Gene Sequence |
| Protein sequence |
