AlfalfaGEDB | MS.gene040993

M. sativa cultivar XinJiangDaYe / MS.gene040993

KEGG: K04424
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF07714.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040993.t1	XP_003589007.2	69.9	103	26	1	1	98	828	930	2.90E-33	151

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040993.t1	Q9FPR3	55.2	96	38	1	1	91	834	929	9.5e-25	114.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040993.t1	G7IB74	69.9	103	26	1	1	98	828	930	2.1e-33	151.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040993.t1	MTR_1g016300	69.903	103	26	1	1	98	828	930	3.23e-45	155
MS.gene040993.t1	MTR_1g081790	56.250	96	37	1	1	91	854	949	3.83e-30	112
MS.gene040993.t1	MTR_2g034020	54.839	93	37	1	1	88	631	723	8.44e-28	105
MS.gene040993.t1	MTR_6g079540	45.833	96	47	1	1	91	825	920	1.24e-27	105
MS.gene040993.t1	MTR_6g079540	45.833	96	47	1	1	91	577	672	1.05e-26	102
MS.gene040993.t1	MTR_8g107000	47.525	101	45	2	1	96	894	991	5.24e-26	100
MS.gene040993.t1	MTR_4g122160	55.556	81	31	1	1	76	625	705	1.61e-25	99.0
MS.gene040993.t1	MTR_5g010730	48.864	88	40	1	1	83	588	675	3.61e-25	98.2
MS.gene040993.t1	MTR_5g010730	48.864	88	40	1	1	83	643	730	4.37e-25	97.8
MS.gene040993.t1	MTR_1g061190	48.864	88	40	1	1	83	722	809	2.79e-23	92.8
MS.gene040993.t1	MTR_1g081790	61.111	72	23	1	1	67	854	925	4.70e-23	92.0
MS.gene040993.t1	MTR_3g100090	40.952	105	55	2	1	98	820	924	1.62e-22	90.5
MS.gene040993.t1	MTR_1g050690	48.352	91	42	1	1	86	584	674	2.24e-21	87.4
MS.gene040993.t1	MTR_1g026160	38.947	95	53	1	1	90	307	401	1.50e-20	84.7
MS.gene040993.t1	MTR_5g066490	40.659	91	49	1	1	86	813	903	8.23e-20	82.8
MS.gene040993.t1	MTR_3g114900	36.842	95	55	1	1	90	432	526	1.02e-17	77.0
MS.gene040993.t1	MTR_4g132860	40.000	90	49	1	1	85	247	336	4.03e-17	74.7
MS.gene040993.t1	MTR_7g072710	40.845	71	42	0	14	84	237	307	4.64e-17	74.7
MS.gene040993.t1	MTR_7g072710	40.845	71	42	0	14	84	200	270	6.75e-17	73.9
MS.gene040993.t1	MTR_5g023150	35.789	95	56	1	1	90	360	454	2.89e-16	72.8
MS.gene040993.t1	MTR_5g023150	35.789	95	56	1	1	90	457	551	4.30e-16	72.4
MS.gene040993.t1	MTR_1g113960	35.417	96	57	1	1	91	463	558	8.81e-16	71.2
MS.gene040993.t1	MTR_1g113960	35.789	95	56	1	1	90	463	557	1.83e-15	70.5
MS.gene040993.t1	MTR_6g033130	38.462	91	47	2	1	83	1141	1230	4.45e-14	66.6
MS.gene040993.t1	MTR_1g099110	36.905	84	47	2	1	79	445	527	1.64e-13	64.7
MS.gene040993.t1	MTR_1g099110	36.905	84	47	2	1	79	428	510	1.86e-13	64.7
MS.gene040993.t1	MTR_8g100085	35.227	88	52	1	1	83	300	387	3.20e-13	63.9
MS.gene040993.t1	MTR_3g034100	35.227	88	52	1	1	83	249	336	4.16e-13	63.5
MS.gene040993.t1	MTR_3g078000	37.500	88	50	1	1	83	224	311	1.29e-12	62.0
MS.gene040993.t1	MTR_2g092980	40.909	88	43	3	1	83	686	769	1.64e-12	62.0
MS.gene040993.t1	MTR_2g092980	40.909	88	43	3	1	83	667	750	1.67e-12	62.0
MS.gene040993.t1	MTR_2g092980	40.909	88	43	3	1	83	703	786	1.71e-12	62.0
MS.gene040993.t1	MTR_2g092980	40.909	88	43	3	1	83	722	805	1.71e-12	62.0
MS.gene040993.t1	MTR_4g080030	42.045	88	42	3	1	83	712	795	4.95e-12	60.8
MS.gene040993.t1	MTR_4g088750	35.556	90	53	1	1	85	369	458	6.04e-12	60.5
MS.gene040993.t1	MTR_6g004440	38.889	72	43	1	14	85	180	250	1.52e-11	58.9
MS.gene040993.t1	MTR_4g105770	32.222	90	56	1	1	85	225	314	1.79e-11	58.9
MS.gene040993.t1	MTR_4g106980	32.967	91	56	1	1	86	276	366	2.90e-11	58.5
MS.gene040993.t1	MTR_2g461180	35.556	90	51	2	1	83	1301	1390	3.02e-11	58.5
MS.gene040993.t1	MTR_8g442400	36.264	91	49	2	1	83	1560	1649	3.22e-11	58.5
MS.gene040993.t1	MTR_4g088745	32.222	90	56	1	1	85	213	302	3.26e-11	58.2
MS.gene040993.t1	MTR_4g088755	32.222	90	56	1	1	85	208	297	3.82e-11	58.2
MS.gene040993.t1	MTR_4g128650	35.556	90	51	2	1	83	985	1074	6.97e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040993.t1	AT5G11850	50.980	102	45	1	1	97	774	875	3.04e-31	115
MS.gene040993.t1	AT1G08720	55.208	96	38	1	1	91	834	929	9.78e-30	111
MS.gene040993.t1	AT1G18160	49.462	93	42	1	1	88	880	972	4.78e-27	103
MS.gene040993.t1	AT1G67890	49.462	93	42	1	1	88	651	743	1.40e-26	102
MS.gene040993.t1	AT1G73660	47.525	101	45	2	1	96	913	1010	1.02e-25	99.8
MS.gene040993.t1	AT5G49470	48.387	93	43	1	1	88	717	809	6.29e-25	97.4
MS.gene040993.t1	AT5G49470	48.387	93	43	1	1	88	529	621	9.33e-25	97.1
MS.gene040993.t1	AT5G49470	48.387	93	43	1	1	88	656	748	1.16e-24	96.7
MS.gene040993.t1	AT5G49470	48.387	93	43	1	1	88	369	461	1.69e-24	95.9
MS.gene040993.t1	AT5G49470	48.387	93	43	1	1	88	630	722	1.86e-24	96.3
MS.gene040993.t1	AT1G08720	59.722	72	24	1	1	67	834	905	3.56e-24	95.5
MS.gene040993.t1	AT3G06640	47.312	93	44	1	1	88	611	703	7.73e-24	94.4
MS.gene040993.t1	AT3G06640	47.312	93	44	1	1	88	611	703	7.73e-24	94.4
MS.gene040993.t1	AT3G06640	47.312	93	44	1	1	88	611	703	8.87e-24	94.4
MS.gene040993.t1	AT4G23050	50.000	88	39	1	1	83	632	719	1.19e-23	94.0
MS.gene040993.t1	AT4G23050	50.000	88	39	1	1	83	633	720	1.22e-23	94.0
MS.gene040993.t1	AT3G06630	46.237	93	45	1	1	88	599	691	1.47e-22	90.9
MS.gene040993.t1	AT3G06620	44.086	93	47	1	1	88	659	751	3.48e-22	89.7
MS.gene040993.t1	AT5G03730	52.273	88	37	1	1	83	718	805	6.37e-22	89.0
MS.gene040993.t1	AT5G03730	52.273	88	37	1	1	83	718	805	6.37e-22	89.0
MS.gene040993.t1	AT1G18160	54.167	72	28	1	1	67	880	951	4.58e-21	86.7
MS.gene040993.t1	AT4G24480	40.659	91	49	1	1	86	836	926	1.93e-20	84.7
MS.gene040993.t1	AT4G24480	40.659	91	49	1	1	86	836	926	1.95e-20	84.7
MS.gene040993.t1	AT4G24480	40.659	91	49	1	1	86	841	931	1.99e-20	84.7
MS.gene040993.t1	AT1G67890	49.296	71	31	1	1	66	523	593	1.08e-17	77.0
MS.gene040993.t1	AT1G67890	49.296	71	31	1	1	66	651	721	1.24e-17	76.6
MS.gene040993.t1	AT1G67890	49.296	71	31	1	1	66	651	721	1.24e-17	76.6
MS.gene040993.t1	AT4G35780	35.789	95	56	1	1	90	454	548	3.62e-17	75.5
MS.gene040993.t1	AT3G06630	50.704	71	30	1	1	66	599	669	6.97e-17	74.7
MS.gene040993.t1	AT1G62400	37.778	90	51	1	1	85	253	342	1.62e-16	73.2
MS.gene040993.t1	AT5G01850	40.845	71	42	0	14	84	210	280	1.64e-16	72.8
MS.gene040993.t1	AT5G01850	40.845	71	42	0	14	84	234	304	2.06e-16	72.8
MS.gene040993.t1	AT3G58640	43.182	88	41	3	1	83	718	801	1.20e-15	70.9
MS.gene040993.t1	AT3G58640	43.182	88	41	3	1	83	718	801	1.20e-15	70.9
MS.gene040993.t1	AT4G38470	35.789	95	56	1	1	90	452	546	3.50e-15	69.7
MS.gene040993.t1	AT4G38470	36.264	91	53	1	1	86	452	542	3.87e-15	69.7
MS.gene040993.t1	AT2G17700	34.737	95	57	1	1	90	448	542	6.94e-14	65.9
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT4G31170	34.091	88	53	1	1	83	298	385	6.97e-13	63.2
MS.gene040993.t1	AT2G42640	43.182	88	41	3	1	83	690	773	8.13e-13	63.2
MS.gene040993.t1	AT2G42640	43.182	88	41	3	1	83	652	735	8.51e-13	62.8
MS.gene040993.t1	AT2G31010	40.909	88	43	3	1	83	684	767	1.61e-12	62.0
MS.gene040993.t1	AT2G31010	40.909	88	43	3	1	83	684	767	1.61e-12	62.0
MS.gene040993.t1	AT5G57610	36.667	90	50	2	1	83	955	1044	5.11e-12	60.8
MS.gene040993.t1	AT3G63260	36.264	91	53	1	1	86	275	365	1.48e-11	59.3
MS.gene040993.t1	AT5G58950	33.333	90	55	1	1	85	376	465	1.58e-11	59.3
MS.gene040993.t1	AT2G24360	32.955	88	54	1	1	83	297	384	3.10e-11	58.5
MS.gene040993.t1	AT1G04700	31.868	91	53	2	1	83	940	1029	6.12e-11	57.8
MS.gene040993.t1	AT1G04700	31.868	91	53	2	1	83	940	1029	6.12e-11	57.8

Find 27 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGCATACTTACTTCTTAT+TGG	0.164080	1.1:-38910363	None:intergenic
CTGAAATAATACGCGATTGC+TGG	0.325647	1.1:+38910591	MS.gene040993:CDS
ACTGCTGGATCCACTTCTTC+TGG	0.362239	1.1:-38910566	None:intergenic
GGCAAGTTCACCAACACTTC+TGG	0.381021	1.1:-38910338	None:intergenic
TCTTAACCGTGTTCGCCGAT+TGG	0.410547	1.1:+38910752	MS.gene040993:CDS
GAAAATCCCATGGCATGGTA+TGG	0.418133	1.1:+38910490	MS.gene040993:CDS
CGTATTATTTCAGCTACTGC+TGG	0.440994	1.1:-38910581	None:intergenic
CATACTTACTTCTTATTGGA+AGG	0.480954	1.1:-38910359	None:intergenic
GCATGGTATGGGCCCTATGC+AGG	0.496062	1.1:+38910502	MS.gene040993:CDS
CGATTGGTTGTCTGTAAGAC+GGG	0.505063	1.1:+38910768	MS.gene040993:CDS
CCGATTGGTTGTCTGTAAGA+CGG	0.518332	1.1:+38910767	MS.gene040993:CDS
TCTTGAAATTCCAGAAGAAG+TGG	0.521791	1.1:+38910556	MS.gene040993:CDS
ACATCGTTAATAACCTGCAT+AGG	0.527513	1.1:-38910515	None:intergenic
AAAATCCCATGGCATGGTAT+GGG	0.536448	1.1:+38910491	MS.gene040993:CDS
AGACAACCAATCGGCGAACA+CGG	0.541343	1.1:-38910758	None:intergenic
ACGAGATATCAGCTCTGAGA+AGG	0.568387	1.1:-38910730	None:intergenic
GAGAAGGATGGCCGACAGCA+TGG	0.580375	1.1:-38910714	None:intergenic
CATCGTTAATAACCTGCATA+GGG	0.594517	1.1:-38910514	None:intergenic
CCGTCTTACAGACAACCAAT+CGG	0.596277	1.1:-38910767	None:intergenic
TAGGGCCCATACCATGCCAT+GGG	0.599805	1.1:-38910496	None:intergenic
ACCAGGAAAATCCCATGGCA+TGG	0.616994	1.1:+38910485	MS.gene040993:CDS
GATATCAGCTCTGAGAAGGA+TGG	0.619267	1.1:-38910726	None:intergenic
ATAGGGCCCATACCATGCCA+TGG	0.619438	1.1:-38910497	None:intergenic
GATGTAGCGAGCCATGCTGT+CGG	0.629581	1.1:+38910703	MS.gene040993:intron
GTGGATGGCACCAGAAGTGT+TGG	0.642852	1.1:+38910328	None:intergenic
TCTTGTGGGAGTTAACTACC+AGG	0.662165	1.1:+38910468	MS.gene040993:CDS
TAACTACCAGGAAAATCCCA+TGG	0.665674	1.1:+38910480	MS.gene040993:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAACATTGAAAACATAAAT+GGG	-	chr1.1:38910636-38910655	None:intergenic	15.0%
!!	AAACATTGAAAACATAAATG+GGG	-	chr1.1:38910635-38910654	None:intergenic	20.0%
!!	GAAAACATTGAAAACATAAA+TGG	-	chr1.1:38910637-38910656	None:intergenic	20.0%
!	AAAAGCAGATATAAGAGTTT+AGG	+	chr1.1:38910390-38910409	MS.gene040993:intron	25.0%
!!	ATGTGTGATGTATATAGTTT+TGG	+	chr1.1:38910440-38910459	MS.gene040993:intron	25.0%
	CATACTTACTTCTTATTGGA+AGG	-	chr1.1:38910362-38910381	None:intergenic	30.0%
	CTTTAAATTGACTGTGCTAT+TGG	+	chr1.1:38910656-38910675	MS.gene040993:intron	30.0%
	GAAGCATACTTACTTCTTAT+TGG	-	chr1.1:38910366-38910385	None:intergenic	30.0%
	TAAGAAGTAAGTATGCTTCA+TGG	+	chr1.1:38910367-38910386	MS.gene040993:intron	30.0%
	ACATCGTTAATAACCTGCAT+AGG	-	chr1.1:38910518-38910537	None:intergenic	35.0%
	CATCGTTAATAACCTGCATA+GGG	-	chr1.1:38910517-38910536	None:intergenic	35.0%
	TCTTGAAATTCCAGAAGAAG+TGG	+	chr1.1:38910556-38910575	MS.gene040993:CDS	35.0%
!!!	ATAGTTTTGGTGTGATCTTG+TGG	+	chr1.1:38910453-38910472	MS.gene040993:intron	35.0%
!!!	TAGTTTTGGTGTGATCTTGT+GGG	+	chr1.1:38910454-38910473	MS.gene040993:intron	35.0%
	AAAATCCCATGGCATGGTAT+GGG	+	chr1.1:38910491-38910510	MS.gene040993:CDS	40.0%
	CGTATTATTTCAGCTACTGC+TGG	-	chr1.1:38910584-38910603	None:intergenic	40.0%
	CTGAAATAATACGCGATTGC+TGG	+	chr1.1:38910591-38910610	MS.gene040993:CDS	40.0%
	TAACTACCAGGAAAATCCCA+TGG	+	chr1.1:38910480-38910499	MS.gene040993:CDS	40.0%
	CCGTCTTACAGACAACCAAT+CGG	-	chr1.1:38910770-38910789	None:intergenic	45.0%
	GAAAATCCCATGGCATGGTA+TGG	+	chr1.1:38910490-38910509	MS.gene040993:CDS	45.0%
	TCTTGTGGGAGTTAACTACC+AGG	+	chr1.1:38910468-38910487	MS.gene040993:CDS	45.0%
!	CCGATTGGTTGTCTGTAAGA+CGG	+	chr1.1:38910767-38910786	MS.gene040993:CDS	45.0%
!	CGATTGGTTGTCTGTAAGAC+GGG	+	chr1.1:38910768-38910787	MS.gene040993:CDS	45.0%
!!	ACGAGATATCAGCTCTGAGA+AGG	-	chr1.1:38910733-38910752	None:intergenic	45.0%
!!	GATATCAGCTCTGAGAAGGA+TGG	-	chr1.1:38910729-38910748	None:intergenic	45.0%
	ACCAGGAAAATCCCATGGCA+TGG	+	chr1.1:38910485-38910504	MS.gene040993:CDS	50.0%
	ACTGCTGGATCCACTTCTTC+TGG	-	chr1.1:38910569-38910588	None:intergenic	50.0%
	AGACAACCAATCGGCGAACA+CGG	-	chr1.1:38910761-38910780	None:intergenic	50.0%
	GGCAAGTTCACCAACACTTC+TGG	-	chr1.1:38910341-38910360	None:intergenic	50.0%
	TCTTAACCGTGTTCGCCGAT+TGG	+	chr1.1:38910752-38910771	MS.gene040993:CDS	50.0%
!	ACCATGCCATGGGATTTTCC+TGG	-	chr1.1:38910489-38910508	None:intergenic	50.0%
	ATAGGGCCCATACCATGCCA+TGG	-	chr1.1:38910500-38910519	None:intergenic	55.0%
	GATGTAGCGAGCCATGCTGT+CGG	+	chr1.1:38910703-38910722	MS.gene040993:intron	55.0%
	TAGGGCCCATACCATGCCAT+GGG	-	chr1.1:38910499-38910518	None:intergenic	55.0%
	GAGAAGGATGGCCGACAGCA+TGG	-	chr1.1:38910717-38910736	None:intergenic	60.0%
	GCATGGTATGGGCCCTATGC+AGG	+	chr1.1:38910502-38910521	MS.gene040993:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	38910332	38910803	38910332	ID=MS.gene040993
chr1.1	mRNA	38910332	38910803	38910332	ID=MS.gene040993.t1;Parent=MS.gene040993
chr1.1	exon	38910332	38910372	38910332	ID=MS.gene040993.t1.exon1;Parent=MS.gene040993.t1
chr1.1	CDS	38910332	38910372	38910332	ID=cds.MS.gene040993.t1;Parent=MS.gene040993.t1
chr1.1	exon	38910457	38910618	38910457	ID=MS.gene040993.t1.exon2;Parent=MS.gene040993.t1
chr1.1	CDS	38910457	38910618	38910457	ID=cds.MS.gene040993.t1;Parent=MS.gene040993.t1
chr1.1	exon	38910710	38910803	38910710	ID=MS.gene040993.t1.exon3;Parent=MS.gene040993.t1
chr1.1	CDS	38910710	38910803	38910710	ID=cds.MS.gene040993.t1;Parent=MS.gene040993.t1

Gene Sequence

>MS.gene040993

ATGGCACCAGAAGTGTTGGTGAACTTGCCTTCCAATAAGAAGTAAGTATGCTTCATGGAAAAGCAGATATAAGAGTTTAGGATGTAATATTTAACGTTTGCATGATGCATGTGTGATGTATATAGTTTTGGTGTGATCTTGTGGGAGTTAACTACCAGGAAAATCCCATGGCATGGTATGGGCCCTATGCAGGTTATTAACGATGTTGTGTTCGAAAATAAACGTCTTGAAATTCCAGAAGAAGTGGATCCAGCAGTAGCTGAAATAATACGCGATTGCTGGAAAAAGTAAGTATTAATTCCCCATTTATGTTTTCAATGTTTTCTTTAAATTGACTGTGCTATTGGATTGATTTTAGTTGTGTTGTTTATGATGTAGCGAGCCATGCTGTCGGCCATCCTTCTCAGAGCTGATATCTCGTCTTAACCGTGTTCGCCGATTGGTTGTCTGTAAGACGGGCTCTACACATTAA

Protein sequence

>MS.gene040993.t1

MAPEVLVNLPSNKNFGVILWELTTRKIPWHGMGPMQVINDVVFENKRLEIPEEVDPAVAEIIRDCWKNEPCCRPSFSELISRLNRVRRLVVCKTGSTH