AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041096

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041096.t1 MTR_5g017500 84.861 251 16 4 1 251 1 229 1.02e-144 404
MS.gene041096.t1 MTR_4g117990 45.872 109 57 1 15 123 36 142 4.06e-27 107
MS.gene041096.t1 MTR_7g081190 49.020 102 49 2 20 119 43 143 9.74e-22 93.2
MS.gene041096.t1 MTR_1g061600 31.111 135 74 4 23 144 133 261 6.89e-16 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041096.t1 AT3G24860 33.829 269 135 6 12 250 54 309 2.36e-43 149
MS.gene041096.t1 AT2G44730 44.348 115 54 2 10 120 51 159 1.22e-22 95.5
MS.gene041096.t1 AT3G10030 36.634 101 58 2 23 123 161 255 2.91e-15 75.1
MS.gene041096.t1 AT3G54390 28.692 237 130 8 23 235 54 275 3.24e-15 74.3
MS.gene041096.t1 AT3G54390 28.814 236 129 8 23 234 38 258 3.25e-15 73.9
MS.gene041096.t1 AT3G10030 36.634 101 58 2 23 123 161 255 3.29e-15 75.1
MS.gene041096.t1 AT3G58630 31.624 117 67 2 23 129 26 139 3.87e-14 71.2
MS.gene041096.t1 AT3G58630 31.624 117 67 2 23 129 26 139 4.09e-14 71.2

Find 80 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTGTTACTGTTGGTTTC+TGG 0.153076 5.2:-9600912 None:intergenic
TGATGAGGTTAATGGTTTCT+TGG 0.248878 5.2:-9600501 None:intergenic
GTAGGCTCTGATGAGGTTAA+TGG 0.265129 5.2:-9600509 None:intergenic
GATTCTATTGGAAAGGCATT+TGG 0.282980 5.2:+9601150 MS.gene041096:CDS
TGGAGGATTGTTGATTCTAT+TGG 0.285128 5.2:+9601138 MS.gene041096:CDS
ACGACATTGAAGAGCGGTTT+TGG 0.311108 5.2:-9600638 None:intergenic
TCTTGGTGGGTCCATGGAAT+GGG 0.347722 5.2:-9600484 None:intergenic
TTCTTGGTGGGTCCATGGAA+TGG 0.347924 5.2:-9600485 None:intergenic
CCGTCGCTATCGTCGGAGAT+AGG 0.363885 5.2:-9600823 None:intergenic
GATTAGAGATGGAGAATAAG+AGG 0.371813 5.2:+9601091 MS.gene041096:CDS
ATTCTATTGGAAAGGCATTT+GGG 0.373738 5.2:+9601151 MS.gene041096:CDS
TTTCAGATCGGTGGCGTTGA+CGG 0.386482 5.2:-9600678 None:intergenic
ATGGGTTGAGGTTTCTTTGT+GGG 0.390802 5.2:-9600466 None:intergenic
GAAATGGTATTCACTCAAAC+GGG 0.393548 5.2:+9600537 MS.gene041096:CDS
GCCCACTCCGCGGCAGTCAA+TGG 0.394607 5.2:+9600560 MS.gene041096:CDS
GAAAGAGGTCGGACGGAGAT+AGG 0.403361 5.2:-9600790 None:intergenic
CCGCCGCTTCTTCTCGATCC+TGG 0.407620 5.2:+9600725 MS.gene041096:CDS
CCCATTGACTGCCGCGGAGT+GGG 0.424106 5.2:-9600561 None:intergenic
TGCAGAGAGGATTGTAGGGT+TGG 0.427539 5.2:+9601026 MS.gene041096:CDS
ATGGTTTCTTGGTGGGTCCA+TGG 0.435098 5.2:-9600490 None:intergenic
ATAGTGCTGCTAGGTCTGTT+AGG 0.441997 5.2:+9600863 MS.gene041096:CDS
GGAGGAGGAAGACGAAGAGG+AGG 0.442580 5.2:+9600963 MS.gene041096:CDS
TGGGTCCATGGAATGGGTTG+AGG 0.452120 5.2:-9600478 None:intergenic
CCCACTCCGCGGCAGTCAAT+GGG 0.457508 5.2:+9600561 MS.gene041096:CDS
AACTGATGATGAAGAGGAGG+AGG 0.459365 5.2:+9600948 MS.gene041096:CDS
CGTTGTTGTCTTGTTACTGT+TGG 0.464913 5.2:-9600919 None:intergenic
GGATCGAGAAGAAGCGGCGG+AGG 0.465439 5.2:-9600722 None:intergenic
AGAGAGGTTTAGATTAGAGA+TGG 0.466781 5.2:+9601080 MS.gene041096:CDS
TTGAGGTTTCTTTGTGGGAA+GGG 0.468718 5.2:-9600461 None:intergenic
TCAGAGCCTACCAAGAGAAA+TGG 0.468909 5.2:+9600521 MS.gene041096:CDS
TCCCATTGACTGCCGCGGAG+TGG 0.479922 5.2:-9600562 None:intergenic
AGAAATGGTATTCACTCAAA+CGG 0.486979 5.2:+9600536 MS.gene041096:CDS
GTATGATACTTGAATCTCAA+TGG 0.493726 5.2:+9601118 MS.gene041096:CDS
GAGGAGGAAGACGAAGAGGA+GGG 0.496326 5.2:+9600964 MS.gene041096:CDS
GAGGTTAATGGTTTCTTGGT+GGG 0.497980 5.2:-9600497 None:intergenic
AGGGAAAGAGTGAGTTCTGC+TGG 0.504699 5.2:-9600442 None:intergenic
CGTCGCTATCGTCGGAGATA+GGG 0.505081 5.2:-9600822 None:intergenic
TGAATACCATTTCTCTTGGT+AGG 0.515604 5.2:-9600527 None:intergenic
TGAGTGAATACCATTTCTCT+TGG 0.519977 5.2:-9600531 None:intergenic
GATTGTTGATTCTATTGGAA+AGG 0.528242 5.2:+9601143 MS.gene041096:CDS
AGACGAAGAGGAGGGAGTGA+TGG 0.530323 5.2:+9600972 MS.gene041096:CDS
TGAGGTTAATGGTTTCTTGG+TGG 0.535347 5.2:-9600498 None:intergenic
TCTTCAATGTCGTCATAAGA+TGG 0.539576 5.2:+9600648 MS.gene041096:CDS
AATGGGTTGAGGTTTCTTTG+TGG 0.544865 5.2:-9600467 None:intergenic
AGAAACCTCAACCCATTCCA+TGG 0.546082 5.2:+9600473 MS.gene041096:CDS
TCTCTTGGTAGGCTCTGATG+AGG 0.553443 5.2:-9600516 None:intergenic
TAAGAAGATGGAGATGATGA+AGG 0.556347 5.2:+9601053 MS.gene041096:CDS
AGAGGAGGAGGAAGACGAAG+AGG 0.557060 5.2:+9600960 MS.gene041096:CDS
CGGCAGTCAATGGGAAGAAG+TGG 0.558439 5.2:+9600570 MS.gene041096:CDS
ATAGGGTGCTTCTGAGAAAG+AGG 0.564002 5.2:-9600805 None:intergenic
GTTGAGGTTTCTTTGTGGGA+AGG 0.565690 5.2:-9600462 None:intergenic
GGTCGGACGGAGATAGGTAA+AGG 0.569635 5.2:-9600784 None:intergenic
AGGGTTGGAAAATAAGAAGA+TGG 0.571203 5.2:+9601041 MS.gene041096:CDS
CTTCTTCCCATTGACTGCCG+CGG 0.577676 5.2:-9600567 None:intergenic
AGAAGCGGCGGAGGAAGTAG+CGG 0.578291 5.2:-9600713 None:intergenic
CTGCTAGGTCTGTTAGGTCA+AGG 0.578720 5.2:+9600869 MS.gene041096:CDS
CCGACGATAGCGACGGTGAG+AGG 0.582725 5.2:+9600830 MS.gene041096:CDS
CCTCTCACCGTCGCTATCGT+CGG 0.588877 5.2:-9600830 None:intergenic
CGGAGGAAGTAGCGGTGAGG+CGG 0.592434 5.2:-9600705 None:intergenic
CGACGATAGCGACGGTGAGA+GGG 0.604292 5.2:+9600831 MS.gene041096:CDS
GAGTTCTGCTGGTGAAGCGG+TGG 0.606218 5.2:-9600431 None:intergenic
CATGAGACGAAAATACTGCC+AGG 0.607104 5.2:-9600743 None:intergenic
CTTCTGAGAAAGAGGTCGGA+CGG 0.613702 5.2:-9600797 None:intergenic
CTGCCAGGATCGAGAAGAAG+CGG 0.618066 5.2:-9600728 None:intergenic
GGTGCTTCTGAGAAAGAGGT+CGG 0.636676 5.2:-9600801 None:intergenic
AGATGATGAAGGAAACAGAG+AGG 0.638850 5.2:+9601064 MS.gene041096:CDS
CTCAAACGGGGCCCACTCCG+CGG 0.643190 5.2:+9600550 MS.gene041096:CDS
TGATGAAACTGATGATGAAG+AGG 0.652723 5.2:+9600942 MS.gene041096:CDS
TGATACTTGAATCTCAATGG+AGG 0.662965 5.2:+9601121 MS.gene041096:CDS
CTCATGGATGATCTTGAACG+CGG 0.669974 5.2:+9600760 MS.gene041096:CDS
CCAGGATCGAGAAGAAGCGG+CGG 0.670197 5.2:-9600725 None:intergenic
AGTGAGTTCTGCTGGTGAAG+CGG 0.672238 5.2:-9600434 None:intergenic
TGAAACTGATGATGAAGAGG+AGG 0.676552 5.2:+9600945 MS.gene041096:CDS
CTTATGACGACATTGAAGAG+CGG 0.678566 5.2:-9600644 None:intergenic
CCTATCTCCGACGATAGCGA+CGG 0.701116 5.2:+9600823 MS.gene041096:CDS
GTAATCGTAGCCGCAACGAG+CGG 0.705311 5.2:-9600605 None:intergenic
CGGCGGAGGAAGTAGCGGTG+AGG 0.707049 5.2:-9600708 None:intergenic
GATGGCGAAGAGTTTGACAG+CGG 0.738106 5.2:+9600990 MS.gene041096:CDS
AAATGGTATTCACTCAAACG+GGG 0.751020 5.2:+9600538 MS.gene041096:CDS
GTAGTCGTAGCCGCTCGTTG+CGG 0.770618 5.2:+9600595 MS.gene041096:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATGGTATTCACTCAAA+CGG + chr5.2:9600536-9600555 MS.gene041096:CDS 30.0%
GTATGATACTTGAATCTCAA+TGG + chr5.2:9601118-9601137 MS.gene041096:CDS 30.0%
!! ATTCTATTGGAAAGGCATTT+GGG + chr5.2:9601151-9601170 MS.gene041096:CDS 30.0%
!! GATTGTTGATTCTATTGGAA+AGG + chr5.2:9601143-9601162 MS.gene041096:CDS 30.0%
AAATGGTATTCACTCAAACG+GGG + chr5.2:9600538-9600557 MS.gene041096:CDS 35.0%
AGAGAGGTTTAGATTAGAGA+TGG + chr5.2:9601080-9601099 MS.gene041096:CDS 35.0%
AGGGTTGGAAAATAAGAAGA+TGG + chr5.2:9601041-9601060 MS.gene041096:CDS 35.0%
GAAATGGTATTCACTCAAAC+GGG + chr5.2:9600537-9600556 MS.gene041096:CDS 35.0%
GATTAGAGATGGAGAATAAG+AGG + chr5.2:9601091-9601110 MS.gene041096:CDS 35.0%
TAAGAAGATGGAGATGATGA+AGG + chr5.2:9601053-9601072 MS.gene041096:CDS 35.0%
TCTTCAATGTCGTCATAAGA+TGG + chr5.2:9600648-9600667 MS.gene041096:CDS 35.0%
TGAATACCATTTCTCTTGGT+AGG - chr5.2:9600530-9600549 None:intergenic 35.0%
TGAGTGAATACCATTTCTCT+TGG - chr5.2:9600534-9600553 None:intergenic 35.0%
TGATACTTGAATCTCAATGG+AGG + chr5.2:9601121-9601140 MS.gene041096:CDS 35.0%
TGATGAGGTTAATGGTTTCT+TGG - chr5.2:9600504-9600523 None:intergenic 35.0%
! TGATGAAACTGATGATGAAG+AGG + chr5.2:9600942-9600961 MS.gene041096:CDS 35.0%
!! GATTCTATTGGAAAGGCATT+TGG + chr5.2:9601150-9601169 MS.gene041096:CDS 35.0%
!! TGGAGGATTGTTGATTCTAT+TGG + chr5.2:9601138-9601157 MS.gene041096:CDS 35.0%
AGATGATGAAGGAAACAGAG+AGG + chr5.2:9601064-9601083 MS.gene041096:CDS 40.0%
CTTATGACGACATTGAAGAG+CGG - chr5.2:9600647-9600666 None:intergenic 40.0%
GAGGTTAATGGTTTCTTGGT+GGG - chr5.2:9600500-9600519 None:intergenic 40.0%
TGAGGTTAATGGTTTCTTGG+TGG - chr5.2:9600501-9600520 None:intergenic 40.0%
TTGAGGTTTCTTTGTGGGAA+GGG - chr5.2:9600464-9600483 None:intergenic 40.0%
! AGTTGAGAAGTTTTGCAGAG+AGG + chr5.2:9601013-9601032 MS.gene041096:CDS 40.0%
! AGTTTTGCAGAGAGGATTGT+AGG + chr5.2:9601021-9601040 MS.gene041096:CDS 40.0%
! ATGGGTTGAGGTTTCTTTGT+GGG - chr5.2:9600469-9600488 None:intergenic 40.0%
! CGTTGTTGTCTTGTTACTGT+TGG - chr5.2:9600922-9600941 None:intergenic 40.0%
! GTTTTGCAGAGAGGATTGTA+GGG + chr5.2:9601022-9601041 MS.gene041096:CDS 40.0%
! TGAAAAAGCATAGTGCTGCT+AGG + chr5.2:9600854-9600873 MS.gene041096:CDS 40.0%
! TGAAACTGATGATGAAGAGG+AGG + chr5.2:9600945-9600964 MS.gene041096:CDS 40.0%
!! AATGGGTTGAGGTTTCTTTG+TGG - chr5.2:9600470-9600489 None:intergenic 40.0%
!! GTCTTGTTACTGTTGGTTTC+TGG - chr5.2:9600915-9600934 None:intergenic 40.0%
!!! ATTGAAGAGCGGTTTTGGAA+GGG - chr5.2:9600636-9600655 None:intergenic 40.0%
AGAAACCTCAACCCATTCCA+TGG + chr5.2:9600473-9600492 MS.gene041096:CDS 45.0%
CATGAGACGAAAATACTGCC+AGG - chr5.2:9600746-9600765 None:intergenic 45.0%
CTCATGGATGATCTTGAACG+CGG + chr5.2:9600760-9600779 MS.gene041096:CDS 45.0%
GTTGAGGTTTCTTTGTGGGA+AGG - chr5.2:9600465-9600484 None:intergenic 45.0%
TCAGAGCCTACCAAGAGAAA+TGG + chr5.2:9600521-9600540 MS.gene041096:CDS 45.0%
! AACTGATGATGAAGAGGAGG+AGG + chr5.2:9600948-9600967 MS.gene041096:CDS 45.0%
! ACGACATTGAAGAGCGGTTT+TGG - chr5.2:9600641-9600660 None:intergenic 45.0%
! ATAGGGTGCTTCTGAGAAAG+AGG - chr5.2:9600808-9600827 None:intergenic 45.0%
! ATAGTGCTGCTAGGTCTGTT+AGG + chr5.2:9600863-9600882 MS.gene041096:CDS 45.0%
! CTGGCAGTATTTTCGTCTCA+TGG + chr5.2:9600744-9600763 MS.gene041096:CDS 45.0%
! GTAGGCTCTGATGAGGTTAA+TGG - chr5.2:9600512-9600531 None:intergenic 45.0%
!! TGAGGCGGTGTTTTTCAGAT+CGG - chr5.2:9600693-9600712 None:intergenic 45.0%
!!! CATTGAAGAGCGGTTTTGGA+AGG - chr5.2:9600637-9600656 None:intergenic 45.0%
AGGGAAAGAGTGAGTTCTGC+TGG - chr5.2:9600445-9600464 None:intergenic 50.0%
AGTGAGTTCTGCTGGTGAAG+CGG - chr5.2:9600437-9600456 None:intergenic 50.0%
CTGCTAGGTCTGTTAGGTCA+AGG + chr5.2:9600869-9600888 MS.gene041096:CDS 50.0%
GATGGCGAAGAGTTTGACAG+CGG + chr5.2:9600990-9601009 MS.gene041096:CDS 50.0%
TGCAGAGAGGATTGTAGGGT+TGG + chr5.2:9601026-9601045 MS.gene041096:CDS 50.0%
! ATGGTTTCTTGGTGGGTCCA+TGG - chr5.2:9600493-9600512 None:intergenic 50.0%
! CTTCTGAGAAAGAGGTCGGA+CGG - chr5.2:9600800-9600819 None:intergenic 50.0%
! GGTGCTTCTGAGAAAGAGGT+CGG - chr5.2:9600804-9600823 None:intergenic 50.0%
! TCTCTTGGTAGGCTCTGATG+AGG - chr5.2:9600519-9600538 None:intergenic 50.0%
! TCTTGGTGGGTCCATGGAAT+GGG - chr5.2:9600487-9600506 None:intergenic 50.0%
! TTCTTGGTGGGTCCATGGAA+TGG - chr5.2:9600488-9600507 None:intergenic 50.0%
! TTTCAGATCGGTGGCGTTGA+CGG - chr5.2:9600681-9600700 None:intergenic 50.0%
AGACGAAGAGGAGGGAGTGA+TGG + chr5.2:9600972-9600991 MS.gene041096:CDS 55.0%
AGAGGAGGAGGAAGACGAAG+AGG + chr5.2:9600960-9600979 MS.gene041096:CDS 55.0%
CCTATCTCCGACGATAGCGA+CGG + chr5.2:9600823-9600842 MS.gene041096:CDS 55.0%
CGGCAGTCAATGGGAAGAAG+TGG + chr5.2:9600570-9600589 MS.gene041096:CDS 55.0%
CGTCGCTATCGTCGGAGATA+GGG - chr5.2:9600825-9600844 None:intergenic 55.0%
CTGCCAGGATCGAGAAGAAG+CGG - chr5.2:9600731-9600750 None:intergenic 55.0%
GAAAGAGGTCGGACGGAGAT+AGG - chr5.2:9600793-9600812 None:intergenic 55.0%
GAGGAGGAAGACGAAGAGGA+GGG + chr5.2:9600964-9600983 MS.gene041096:CDS 55.0%
GGTCGGACGGAGATAGGTAA+AGG - chr5.2:9600787-9600806 None:intergenic 55.0%
GTAATCGTAGCCGCAACGAG+CGG - chr5.2:9600608-9600627 None:intergenic 55.0%
! CTTCTTCCCATTGACTGCCG+CGG - chr5.2:9600570-9600589 None:intergenic 55.0%
!! GGCGGTGTTTTTCAGATCGG+TGG - chr5.2:9600690-9600709 None:intergenic 55.0%
!! TGGGTCCATGGAATGGGTTG+AGG - chr5.2:9600481-9600500 None:intergenic 55.0%
!!! GAAGAGCGGTTTTGGAAGGG+TGG - chr5.2:9600633-9600652 None:intergenic 55.0%
AGAAGCGGCGGAGGAAGTAG+CGG - chr5.2:9600716-9600735 None:intergenic 60.0%
CCAGGATCGAGAAGAAGCGG+CGG - chr5.2:9600728-9600747 None:intergenic 60.0%
CCGTCGCTATCGTCGGAGAT+AGG - chr5.2:9600826-9600845 None:intergenic 60.0%
CCTCTCACCGTCGCTATCGT+CGG - chr5.2:9600833-9600852 None:intergenic 60.0%
CGACGATAGCGACGGTGAGA+GGG + chr5.2:9600831-9600850 MS.gene041096:CDS 60.0%
GAGTTCTGCTGGTGAAGCGG+TGG - chr5.2:9600434-9600453 None:intergenic 60.0%
GGAGGAGGAAGACGAAGAGG+AGG + chr5.2:9600963-9600982 MS.gene041096:CDS 60.0%
GTAGTCGTAGCCGCTCGTTG+CGG + chr5.2:9600595-9600614 MS.gene041096:CDS 60.0%
CCCACTCCGCGGCAGTCAAT+GGG + chr5.2:9600561-9600580 MS.gene041096:CDS 65.0%
CCGACGATAGCGACGGTGAG+AGG + chr5.2:9600830-9600849 MS.gene041096:CDS 65.0%
CCGCCGCTTCTTCTCGATCC+TGG + chr5.2:9600725-9600744 MS.gene041096:CDS 65.0%
CGGAGGAAGTAGCGGTGAGG+CGG - chr5.2:9600708-9600727 None:intergenic 65.0%
GGATCGAGAAGAAGCGGCGG+AGG - chr5.2:9600725-9600744 None:intergenic 65.0%
! CCCATTGACTGCCGCGGAGT+GGG - chr5.2:9600564-9600583 None:intergenic 65.0%
! TCCCATTGACTGCCGCGGAG+TGG - chr5.2:9600565-9600584 None:intergenic 65.0%
CGGCGGAGGAAGTAGCGGTG+AGG - chr5.2:9600711-9600730 None:intergenic 70.0%
CTCAAACGGGGCCCACTCCG+CGG + chr5.2:9600550-9600569 MS.gene041096:CDS 70.0%
GCCCACTCCGCGGCAGTCAA+TGG + chr5.2:9600560-9600579 MS.gene041096:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 9600427 9601182 9600427 ID=MS.gene041096
chr5.2 mRNA 9600427 9601182 9600427 ID=MS.gene041096.t1;Parent=MS.gene041096
chr5.2 exon 9600427 9601182 9600427 ID=MS.gene041096.t1.exon1;Parent=MS.gene041096.t1
chr5.2 CDS 9600427 9601182 9600427 ID=cds.MS.gene041096.t1;Parent=MS.gene041096.t1
Gene Sequence

>MS.gene041096

ATGGCCACCGCTTCACCAGCAGAACTCACTCTTTCCCTTCCCACAAAGAAACCTCAACCCATTCCATGGACCCACCAAGAAACCATTAACCTCATCAGAGCCTACCAAGAGAAATGGTATTCACTCAAACGGGGCCCACTCCGCGGCAGTCAATGGGAAGAAGTGGCTGTAGTCGTAGCCGCTCGTTGCGGCTACGATTACAACCACCCTTCCAAAACCGCTCTTCAATGTCGTCATAAGATGGAAAAACTCCGTCAACGCCACCGATCTGAAAAACACCGCCTCACCGCTACTTCCTCCGCCGCTTCTTCTCGATCCTGGCAGTATTTTCGTCTCATGGATGATCTTGAACGCGGTCCTTTACCTATCTCCGTCCGACCTCTTTCTCAGAAGCACCCTATCTCCGACGATAGCGACGGTGAGAGGGTGAAAAAGCATAGTGCTGCTAGGTCTGTTAGGTCAAGGAGTATTCATACTGTTCTTAACCAGAAACCAACAGTAACAAGACAACAACGTGATGAAACTGATGATGAAGAGGAGGAGGAAGACGAAGAGGAGGGAGTGATGGCGAAGAGTTTGACAGCGGAGTTGAGAAGTTTTGCAGAGAGGATTGTAGGGTTGGAAAATAAGAAGATGGAGATGATGAAGGAAACAGAGAGGTTTAGATTAGAGATGGAGAATAAGAGGATTCGTATGATACTTGAATCTCAATGGAGGATTGTTGATTCTATTGGAAAGGCATTTGGGTCTTCTTAG
Protein sequence

>MS.gene041096.t1

MATASPAELTLSLPTKKPQPIPWTHQETINLIRAYQEKWYSLKRGPLRGSQWEEVAVVVAARCGYDYNHPSKTALQCRHKMEKLRQRHRSEKHRLTATSSAASSRSWQYFRLMDDLERGPLPISVRPLSQKHPISDDSDGERVKKHSAARSVRSRSIHTVLNQKPTVTRQQRDETDDEEEEEDEEEGVMAKSLTAELRSFAERIVGLENKKMEMMKETERFRLEMENKRIRMILESQWRIVDSIGKAFGSS