AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041128

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041128.t1 MTR_5g017980 96.296 324 12 0 2 325 3 326 0.0 640
MS.gene041128.t1 MTR_5g017980 95.988 324 12 1 2 325 3 325 0.0 633
MS.gene041128.t1 MTR_5g017980 95.988 324 11 2 2 325 3 324 0.0 629
MS.gene041128.t1 MTR_4g086835 40.887 406 148 14 5 324 8 407 1.49e-65 213
MS.gene041128.t1 MTR_4g086835 40.640 406 148 15 5 324 8 406 4.28e-63 206
MS.gene041128.t1 MTR_4g113140 35.179 307 140 8 9 263 6 305 3.21e-39 144
MS.gene041128.t1 MTR_1g093080 32.727 330 145 12 34 293 2 324 2.38e-37 137
MS.gene041128.t1 MTR_5g054300 34.866 261 106 7 39 253 17 259 2.74e-36 134
MS.gene041128.t1 MTR_1g093080 33.692 279 120 10 34 254 2 273 1.73e-30 117
MS.gene041128.t1 MTR_7g088070 51.190 84 31 2 163 246 31 104 1.44e-14 73.2
MS.gene041128.t1 MTR_7g088070 51.190 84 31 2 163 246 31 104 1.53e-14 73.2
MS.gene041128.t1 MTR_3g106220 39.200 125 64 5 191 306 197 318 2.22e-14 74.3
MS.gene041128.t1 MTR_1g093080 28.333 240 107 10 34 215 2 234 2.93e-14 72.0
MS.gene041128.t1 MTR_2g027860 55.714 70 30 1 192 261 49 117 3.15e-14 72.8
MS.gene041128.t1 MTR_4g121020 57.377 61 25 1 191 251 214 273 5.18e-14 73.2
MS.gene041128.t1 MTR_4g121020 57.377 61 25 1 191 251 214 273 5.42e-14 73.2
MS.gene041128.t1 MTR_5g041350 60.000 55 22 0 192 246 15 69 5.50e-14 72.4
MS.gene041128.t1 MTR_4g121020 57.377 61 25 1 191 251 110 169 5.64e-14 73.2
MS.gene041128.t1 MTR_1g090670 58.621 58 24 0 189 246 41 98 1.83e-13 70.1
MS.gene041128.t1 MTR_1g032570 50.000 70 34 1 189 258 128 196 3.23e-13 70.9
MS.gene041128.t1 MTR_4g081710 42.697 89 50 1 192 279 17 105 3.24e-13 70.1
MS.gene041128.t1 MTR_1g032570 48.649 74 37 1 189 262 197 269 3.58e-13 70.9
MS.gene041128.t1 MTR_2g034960 50.000 72 35 1 189 260 208 278 3.69e-13 70.5
MS.gene041128.t1 MTR_1g013170 50.769 65 31 1 187 251 186 249 3.71e-13 70.5
MS.gene041128.t1 MTR_1g053830 50.877 57 28 0 190 246 16 72 4.64e-13 64.7
MS.gene041128.t1 MTR_7g089010 51.667 60 28 1 191 250 90 148 5.94e-13 68.9
MS.gene041128.t1 MTR_2g027800 60.000 55 22 0 192 246 39 93 6.29e-13 68.6
MS.gene041128.t1 MTR_1g080330 53.731 67 30 1 189 255 172 237 6.69e-13 68.6
MS.gene041128.t1 MTR_1g080330 53.731 67 30 1 189 255 172 237 7.44e-13 69.3
MS.gene041128.t1 MTR_2g027800 60.000 55 22 0 192 246 39 93 7.95e-13 68.6
MS.gene041128.t1 MTR_5g014040 34.375 128 56 4 125 251 269 369 1.55e-12 68.6
MS.gene041128.t1 MTR_7g098250 52.239 67 31 1 189 255 259 324 1.65e-12 68.6
MS.gene041128.t1 MTR_6g444980 60.000 55 22 0 192 246 35 89 1.88e-12 67.8
MS.gene041128.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 1.88e-12 67.4
MS.gene041128.t1 MTR_6g444980 60.000 55 22 0 192 246 35 89 2.23e-12 67.4
MS.gene041128.t1 MTR_2g450070 50.000 62 30 1 189 250 210 270 2.64e-12 68.2
MS.gene041128.t1 MTR_2g450070 50.000 62 30 1 189 250 210 270 2.65e-12 68.2
MS.gene041128.t1 MTR_3g064840 46.988 83 40 1 163 245 249 327 2.74e-12 67.8
MS.gene041128.t1 MTR_5g027440 38.000 100 49 2 164 261 8 96 2.78e-12 66.6
MS.gene041128.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 3.13e-12 67.0
MS.gene041128.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 3.45e-12 67.0
MS.gene041128.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 4.87e-12 67.0
MS.gene041128.t1 MTR_4g064730 45.312 64 34 1 188 251 127 189 7.02e-12 65.9
MS.gene041128.t1 MTR_2g086450 38.679 106 50 2 141 246 9 99 8.41e-12 65.9
MS.gene041128.t1 MTR_1g053835 54.386 57 26 0 190 246 256 312 9.02e-12 65.9
MS.gene041128.t1 MTR_6g071625 37.815 119 57 3 158 270 175 282 1.40e-11 65.1
MS.gene041128.t1 MTR_1g112370 59.184 49 20 0 197 245 27 75 1.57e-11 64.7
MS.gene041128.t1 MTR_6g032990 52.727 55 26 0 192 246 42 96 1.65e-11 65.1
MS.gene041128.t1 MTR_5g029470 31.061 132 78 2 153 284 184 302 2.26e-11 64.7
MS.gene041128.t1 MTR_7g093030 53.571 56 26 0 190 245 210 265 2.36e-11 63.9
MS.gene041128.t1 MTR_4g131580 50.000 60 29 1 191 250 199 257 3.43e-11 64.7
MS.gene041128.t1 MTR_7g117705 45.714 70 37 1 191 260 190 258 4.09e-11 64.3
MS.gene041128.t1 MTR_7g093030 53.571 56 26 0 190 245 210 265 4.91e-11 63.5
MS.gene041128.t1 MTR_4g098870 43.939 66 36 1 186 251 304 368 5.13e-11 63.9
MS.gene041128.t1 MTR_4g098870 43.939 66 36 1 186 251 343 407 5.37e-11 63.9
MS.gene041128.t1 MTR_4g098870 43.939 66 36 1 186 251 324 388 5.37e-11 63.9
MS.gene041128.t1 MTR_8g066310 50.820 61 30 0 185 245 166 226 5.77e-11 63.2
MS.gene041128.t1 MTR_4g098870 43.939 66 36 1 186 251 304 368 6.75e-11 63.5
MS.gene041128.t1 MTR_4g098870 43.939 66 36 1 186 251 324 388 6.82e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041128.t1 AT4G37180 40.608 362 154 11 6 329 12 350 2.70e-61 199
MS.gene041128.t1 AT4G37180 39.837 369 154 12 6 329 12 357 1.22e-58 193
MS.gene041128.t1 AT4G37180 44.737 266 101 8 6 242 12 260 1.29e-52 175
MS.gene041128.t1 AT2G03500 38.408 289 128 10 9 250 6 291 5.00e-42 151
MS.gene041128.t1 AT1G68670 35.461 282 118 8 31 256 6 279 1.31e-40 145
MS.gene041128.t1 AT1G25550 35.897 273 118 8 31 256 10 272 8.22e-40 143
MS.gene041128.t1 AT1G49560 56.154 130 37 4 180 290 171 299 7.83e-35 130
MS.gene041128.t1 AT1G25550 34.749 259 113 7 31 242 10 259 1.92e-33 125
MS.gene041128.t1 AT1G13300 85.000 60 9 0 191 250 180 239 4.22e-29 115
MS.gene041128.t1 AT1G49560 70.833 72 12 1 180 242 171 242 4.64e-26 104
MS.gene041128.t1 AT3G25790 81.818 55 10 0 196 250 200 254 1.95e-25 105
MS.gene041128.t1 AT3G25790 80.851 47 9 0 196 242 200 246 2.62e-20 89.0
MS.gene041128.t1 AT2G01060 54.930 71 31 1 185 255 8 77 2.93e-14 72.4
MS.gene041128.t1 AT2G20400 44.828 87 47 1 192 278 231 316 3.39e-14 73.2
MS.gene041128.t1 AT2G20400 46.512 86 43 2 192 277 231 313 1.26e-13 71.6
MS.gene041128.t1 AT2G40970 43.478 92 44 3 159 250 79 162 1.58e-13 69.7
MS.gene041128.t1 AT2G06020 50.000 64 32 0 183 246 77 140 1.60e-13 70.5
MS.gene041128.t1 AT4G13640 61.818 55 21 0 192 246 37 91 1.69e-13 70.5
MS.gene041128.t1 AT4G13640 61.818 55 21 0 192 246 37 91 1.74e-13 70.5
MS.gene041128.t1 AT5G05090 51.667 60 28 1 191 250 80 138 2.05e-13 69.7
MS.gene041128.t1 AT3G10760 51.667 60 28 1 191 250 104 162 3.16e-13 70.1
MS.gene041128.t1 AT3G24120 60.000 55 22 0 192 246 41 95 3.41e-13 69.3
MS.gene041128.t1 AT3G24120 60.000 55 22 0 192 246 41 95 3.67e-13 69.3
MS.gene041128.t1 AT5G59570 46.875 64 33 1 188 251 137 199 4.00e-13 69.3
MS.gene041128.t1 AT5G59570 46.875 64 33 1 188 251 137 199 4.00e-13 69.3
MS.gene041128.t1 AT3G12730 58.182 55 23 0 192 246 23 77 9.02e-13 67.4
MS.gene041128.t1 AT3G16857 54.098 61 27 1 191 251 166 225 9.27e-13 69.3
MS.gene041128.t1 AT3G16857 54.098 61 27 1 191 251 236 295 1.06e-12 69.3
MS.gene041128.t1 AT3G16857 54.098 61 27 1 191 251 236 295 1.16e-12 69.3
MS.gene041128.t1 AT5G58080 49.206 63 31 1 189 251 191 252 1.28e-12 68.9
MS.gene041128.t1 AT3G04030 56.364 55 24 0 192 246 45 99 2.41e-12 67.8
MS.gene041128.t1 AT3G04030 56.364 55 24 0 192 246 45 99 2.88e-12 67.4
MS.gene041128.t1 AT5G06800 54.545 55 25 0 192 246 192 246 3.03e-12 66.6
MS.gene041128.t1 AT1G67710 50.746 67 33 0 185 251 121 187 3.40e-12 67.4
MS.gene041128.t1 AT1G67710 50.746 67 33 0 185 251 112 178 3.53e-12 67.4
MS.gene041128.t1 AT1G79430 60.000 55 22 0 192 246 34 88 3.75e-12 67.0
MS.gene041128.t1 AT1G67710 50.746 67 33 0 185 251 186 252 3.91e-12 67.4
MS.gene041128.t1 AT3G13040 50.000 64 32 0 183 246 232 295 5.15e-12 67.0
MS.gene041128.t1 AT3G13040 50.000 64 32 0 183 246 232 295 5.15e-12 67.0
MS.gene041128.t1 AT3G13040 50.000 64 32 0 183 246 232 295 5.15e-12 67.0
MS.gene041128.t1 AT5G18240 56.364 55 24 0 192 246 45 99 5.45e-12 66.6
MS.gene041128.t1 AT5G18240 56.364 55 24 0 192 246 45 99 5.45e-12 66.6
MS.gene041128.t1 AT3G46640 47.541 61 31 1 191 251 143 202 5.71e-12 66.2
MS.gene041128.t1 AT3G46640 47.541 61 31 1 191 251 143 202 5.71e-12 66.2
MS.gene041128.t1 AT3G04450 50.820 61 30 0 185 245 231 291 5.83e-12 65.9
MS.gene041128.t1 AT4G17695 41.237 97 47 2 177 272 148 235 6.00e-12 66.2
MS.gene041128.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.03e-12 66.6
MS.gene041128.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.03e-12 66.6
MS.gene041128.t1 AT5G18240 56.364 55 24 0 192 246 45 99 6.05e-12 66.6
MS.gene041128.t1 AT5G45580 56.364 55 24 0 192 246 7 61 7.04e-12 65.1
MS.gene041128.t1 AT5G45580 56.364 55 24 0 192 246 23 77 7.28e-12 65.5
MS.gene041128.t1 AT3G46640 47.541 61 31 1 191 251 143 202 7.59e-12 65.9
MS.gene041128.t1 AT5G06800 54.545 55 25 0 192 246 192 246 7.94e-12 66.2
MS.gene041128.t1 AT5G06800 54.545 55 25 0 192 246 192 246 8.54e-12 65.9
MS.gene041128.t1 AT1G32240 38.776 98 51 1 182 279 203 291 8.80e-12 65.9
MS.gene041128.t1 AT1G32240 38.776 98 51 1 182 279 203 291 1.02e-11 65.9
MS.gene041128.t1 AT3G04450 50.820 61 30 0 185 245 231 291 1.17e-11 65.9
MS.gene041128.t1 AT3G04450 50.820 61 30 0 185 245 191 251 1.33e-11 65.5
MS.gene041128.t1 AT3G04450 50.820 61 30 0 185 245 191 251 1.33e-11 65.5
MS.gene041128.t1 AT2G01760 50.000 62 30 1 190 251 133 193 1.46e-11 65.1
MS.gene041128.t1 AT2G01760 50.000 62 30 1 190 251 226 286 1.49e-11 65.5
MS.gene041128.t1 AT2G25180 46.377 69 36 1 191 259 194 261 1.52e-11 65.9
MS.gene041128.t1 AT2G01760 50.000 62 30 1 190 251 198 258 1.68e-11 65.1
MS.gene041128.t1 AT1G69580 50.909 55 27 0 192 246 34 88 1.82e-11 64.7
MS.gene041128.t1 AT1G69580 50.909 55 27 0 192 246 34 88 1.91e-11 64.7
MS.gene041128.t1 AT5G29000 52.632 57 27 0 189 245 228 284 2.03e-11 64.3
MS.gene041128.t1 AT4G31920 40.789 76 44 1 189 264 180 254 2.31e-11 65.1
MS.gene041128.t1 AT5G16560 51.562 64 31 0 182 245 209 272 2.42e-11 64.7
MS.gene041128.t1 AT4G28610 55.556 54 24 0 192 245 225 278 3.09e-11 64.3
MS.gene041128.t1 AT5G29000 52.632 57 27 0 189 245 185 241 4.77e-11 63.5
MS.gene041128.t1 AT5G29000 52.632 57 27 0 189 245 185 241 4.77e-11 63.5
MS.gene041128.t1 AT5G29000 52.632 57 27 0 189 245 228 284 4.96e-11 63.9
MS.gene041128.t1 AT5G42630 53.333 60 28 0 186 245 99 158 6.22e-11 62.0
MS.gene041128.t1 AT4G04605 53.571 56 26 0 190 245 12 67 9.82e-11 61.2
MS.gene041128.t1 AT2G02060 50.000 62 30 1 184 245 22 82 9.91e-11 61.6
MS.gene041128.t1 AT2G02060 50.000 62 30 1 184 245 22 82 9.91e-11 61.6

Find 76 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGGTGGAGGGGTATTTA+TGG 0.217770 5.2:-10095506 MS.gene041128:CDS
CAGCCACTCCTAAACAAATT+AGG 0.237655 5.2:-10094705 MS.gene041128:CDS
ATTCCCAGTTTCTTCAATTC+AGG 0.264265 5.2:-10094474 MS.gene041128:CDS
AAAATTAGTTACCATCATTA+AGG 0.265474 5.2:+10095896 None:intergenic
CCAGCACCTCCTTTGTTTCC+TGG 0.269810 5.2:+10095315 None:intergenic
GCCTCCTGAATTGAAGAAAC+TGG 0.272208 5.2:+10094470 None:intergenic
AGCAATCAGATTGCAGGAAT+TGG 0.302511 5.2:-10094280 MS.gene041128:CDS
AAAAGTTGGGTTGATGAAAA+TGG 0.310662 5.2:-10095756 MS.gene041128:CDS
CTCCTTGGTGGATCTGTTCA+AGG 0.312173 5.2:-10094344 MS.gene041128:CDS
AGCCACTCCTAAACAAATTA+GGG 0.340430 5.2:-10094704 MS.gene041128:CDS
AACCTTTGCAGCAGAGTTCC+AGG 0.347695 5.2:-10095333 MS.gene041128:CDS
AAGAGTGGTGGTGGAAGTTC+TGG 0.370480 5.2:-10095403 MS.gene041128:CDS
CATCCATACTGTTTCTTCCA+TGG 0.384254 5.2:+10094312 None:intergenic
CTCAGAGTCTGTTTCTCCAC+AGG 0.393005 5.2:-10094384 MS.gene041128:CDS
TGCAAAGGTTGCGGCGGCTG+AGG 0.403383 5.2:+10095346 None:intergenic
TTGTGATTATGCAGAAGTAT+AGG 0.413901 5.2:-10094511 MS.gene041128:intron
CCTCTTACTCCTCTTCTCCT+TGG 0.417068 5.2:-10094359 MS.gene041128:CDS
AGAACTTCCACCACCACTCT+TGG 0.423145 5.2:+10095405 None:intergenic
TAAGGAGGAGTATGGAAAGA+GGG 0.427844 5.2:+10095914 None:intergenic
AAGATGAGCAATCAGATTGC+AGG 0.427884 5.2:-10094286 MS.gene041128:CDS
ACCATCATTAAGGAGGAGTA+TGG 0.434982 5.2:+10095906 None:intergenic
ACAAGCTAAGAGCTTCAGTT+TGG 0.440984 5.2:-10095452 MS.gene041128:CDS
AATGGTGGAGGGGTATTTAT+GGG 0.443624 5.2:-10095505 MS.gene041128:CDS
CTTACTCCTCTTCTCCTTGG+TGG 0.445651 5.2:-10094356 MS.gene041128:CDS
ACAATGAAGGTGATGATGAT+AGG 0.445952 5.2:-10095546 MS.gene041128:CDS
ATGGACTAATCAAACAAAAT+CGG 0.449625 5.2:-10095678 MS.gene041128:intron
TTACAGAAGGGTAATGGTGG+AGG 0.452793 5.2:-10095517 MS.gene041128:CDS
AAGCCATGGAAGAAACAGTA+TGG 0.457520 5.2:-10094315 MS.gene041128:CDS
TATTGTCACTCACTTGCAAA+TGG 0.459590 5.2:+10094638 None:intergenic
TCCATACTCCTCCTTAATGA+TGG 0.469852 5.2:-10095907 MS.gene041128:intron
CCAAGGAGAAGAGGAGTAAG+AGG 0.473664 5.2:+10094359 None:intergenic
TTCCCTAATTTGTTTAGGAG+TGG 0.479597 5.2:+10094702 None:intergenic
TTAAGGAGGAGTATGGAAAG+AGG 0.491286 5.2:+10095913 None:intergenic
CAGGAATTGGAAAAGTGATC+AGG 0.500799 5.2:-10094267 MS.gene041128:CDS
ATTTAATGAAAATCTGTTGA+AGG 0.503957 5.2:-10095482 MS.gene041128:CDS
TTCCTGGAACTCTGCTGCAA+AGG 0.504800 5.2:+10095331 None:intergenic
GTTGAAGTGCATCAACAAAT+CGG 0.508453 5.2:+10095275 None:intergenic
TGATGACTTAGTTCATAGCT+TGG 0.508826 5.2:-10095977 MS.gene041128:CDS
ATTGTGTTACAGAAGGGTAA+TGG 0.520220 5.2:-10095523 MS.gene041128:CDS
GGAAATTCCAAGAGTGGTGG+TGG 0.524765 5.2:-10095412 MS.gene041128:CDS
CCTCCTGAATTGAAGAAACT+GGG 0.525229 5.2:+10094471 None:intergenic
CCAGGAAACAAAGGAGGTGC+TGG 0.526510 5.2:-10095315 MS.gene041128:CDS
CCCAGTTTCTTCAATTCAGG+AGG 0.530991 5.2:-10094471 MS.gene041128:CDS
GAACTCTGCTGCAAAGGTTG+CGG 0.536482 5.2:+10095337 None:intergenic
AGGGAAAAGATGCAAGTGGA+TGG 0.538779 5.2:-10094685 MS.gene041128:CDS
TCAGAGTCTGTTTCTCCACA+GGG 0.539092 5.2:-10094383 MS.gene041128:CDS
GGATGAGTTCAGCTCAGCTA+TGG 0.541177 5.2:-10095697 MS.gene041128:CDS
GTTGTTGAGGAAATGAGAAA+CGG 0.545414 5.2:+10096041 None:intergenic
TAATGAAAATCTGTTGAAGG+AGG 0.547757 5.2:-10095479 MS.gene041128:CDS
CTCTGCTGCAAAGGTTGCGG+CGG 0.547761 5.2:+10095340 None:intergenic
TGGTTTGAACAACGTTGTTG+AGG 0.552246 5.2:+10096028 None:intergenic
TGTGAAGAAGATACATCAAA+GGG 0.552624 5.2:-10094413 MS.gene041128:CDS
ACAAAGGAGGTGCTGGTCGT+CGG 0.559002 5.2:-10095308 MS.gene041128:CDS
GATAGGATTGTGTTACAGAA+GGG 0.560504 5.2:-10095529 MS.gene041128:CDS
CAACAACTTGGTGGAGCACA+TGG 0.561362 5.2:-10095256 MS.gene041128:intron
TGATAGGATTGTGTTACAGA+AGG 0.566498 5.2:-10095530 MS.gene041128:CDS
TACAGAAGGGTAATGGTGGA+GGG 0.569592 5.2:-10095516 MS.gene041128:CDS
ACAGATCCACCAAGGAGAAG+AGG 0.569987 5.2:+10094350 None:intergenic
GTTCAAGGTCTCTCAAGCCA+TGG 0.571312 5.2:-10094329 MS.gene041128:CDS
GTTGATGCACTTCAACAACT+TGG 0.579091 5.2:-10095268 MS.gene041128:CDS
ATGTGAAGAAGATACATCAA+AGG 0.582691 5.2:-10094414 MS.gene041128:CDS
ATTAGTTACCATCATTAAGG+AGG 0.585189 5.2:+10095899 None:intergenic
AGCAGAGTTCCAGGAAACAA+AGG 0.588997 5.2:-10095324 MS.gene041128:CDS
TTGATCTTGAGCCATGTACA+AGG 0.594888 5.2:+10094436 None:intergenic
GATGCACTTCAACAACTTGG+TGG 0.610978 5.2:-10095265 MS.gene041128:CDS
AATTAGGGAAAAGATGCAAG+TGG 0.615575 5.2:-10094689 MS.gene041128:CDS
GTGTTACAGAAGGGTAATGG+TGG 0.616384 5.2:-10095520 MS.gene041128:CDS
TAATAAGTTAGCCTTGTACA+TGG 0.624443 5.2:-10094447 MS.gene041128:CDS
TTGGTGTCTGAAGTGAGTCA+TGG 0.630429 5.2:-10095433 MS.gene041128:CDS
ACAGAAGGGTAATGGTGGAG+GGG 0.630851 5.2:-10095515 MS.gene041128:CDS
AGTCATGGAAATTCCAAGAG+TGG 0.640027 5.2:-10095418 MS.gene041128:CDS
AAGTTTCTGAGACATGTCGT+TGG 0.646359 5.2:+10096008 None:intergenic
CATGGAAATTCCAAGAGTGG+TGG 0.649869 5.2:-10095415 MS.gene041128:CDS
AGAGGAGTAAGAGGACCCTG+TGG 0.650364 5.2:+10094368 None:intergenic
AGAGTTCCAGGAAACAAAGG+AGG 0.672803 5.2:-10095321 MS.gene041128:CDS
GACCTTGAACAGATCCACCA+AGG 0.687479 5.2:+10094342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAACAAAAAAAGAAAA+AGG + chr5.2:10094507-10094526 None:intergenic 10.0%
!!! AATTTTCTAATTAATTTTTG+TGG - chr5.2:10094458-10094477 MS.gene041128:CDS 10.0%
!! AAAATCACAATTAAAAGAAA+AGG + chr5.2:10094717-10094736 None:intergenic 15.0%
!! AAATCACAATTAAAAGAAAA+GGG + chr5.2:10094716-10094735 None:intergenic 15.0%
!!! CTCTATTTTATTTTAGATTT+GGG - chr5.2:10095202-10095221 MS.gene041128:intron 15.0%
!!! TCTCTATTTTATTTTAGATT+TGG - chr5.2:10095201-10095220 MS.gene041128:intron 15.0%
!! AAAATTAGTTACCATCATTA+AGG + chr5.2:10094444-10094463 None:intergenic 20.0%
!! AATGATGAAAAAGAAAAAGT+TGG - chr5.2:10094567-10094586 MS.gene041128:intron 20.0%
!! ACTGTGATAATAAAAAGAAT+TGG - chr5.2:10094619-10094638 MS.gene041128:intron 20.0%
!! ATGATGAAAAAGAAAAAGTT+GGG - chr5.2:10094568-10094587 MS.gene041128:intron 20.0%
!! ATTTAATGAAAATCTGTTGA+AGG - chr5.2:10094855-10094874 MS.gene041128:intron 20.0%
!! TCATACAAAGAAACTAATAA+TGG + chr5.2:10095760-10095779 None:intergenic 20.0%
!!! TTATTTTAGATTTGGGAATA+TGG - chr5.2:10095209-10095228 MS.gene041128:intron 20.0%
! ACTAATAATGGTATTAACCA+TGG + chr5.2:10095748-10095767 None:intergenic 25.0%
! ATCAAGATTACATGTTGTTA+GGG - chr5.2:10095468-10095487 MS.gene041128:CDS 25.0%
! TACAAAAAAGAAAACCAGTT+AGG + chr5.2:10095611-10095630 None:intergenic 25.0%
! TGTTAATTAGTTGTGTTATG+CGG - chr5.2:10095140-10095159 MS.gene041128:intron 25.0%
!! ATGGACTAATCAAACAAAAT+CGG - chr5.2:10094659-10094678 MS.gene041128:CDS 25.0%
!!! ATCTTTTCCCTAATTTGTTT+AGG + chr5.2:10095643-10095662 None:intergenic 25.0%
!!! TTGTGGTATTTCTTGTTTTT+TGG - chr5.2:10094475-10094494 MS.gene041128:CDS 25.0%
!!! TTTTTTTGGTAGAACAATGA+AGG - chr5.2:10094778-10094797 MS.gene041128:intron 25.0%
ATAATGGTATTAACCATGGA+TGG + chr5.2:10095744-10095763 None:intergenic 30.0%
ATGTGAAGAAGATACATCAA+AGG - chr5.2:10095923-10095942 MS.gene041128:CDS 30.0%
ATTAGTTACCATCATTAAGG+AGG + chr5.2:10094441-10094460 None:intergenic 30.0%
CAGCAATTTCAATTCCAAAT+TGG + chr5.2:10095243-10095262 None:intergenic 30.0%
GATCAAGATTACATGTTGTT+AGG - chr5.2:10095467-10095486 MS.gene041128:CDS 30.0%
TAATGAAAATCTGTTGAAGG+AGG - chr5.2:10094858-10094877 MS.gene041128:intron 30.0%
TGTGAAGAAGATACATCAAA+GGG - chr5.2:10095924-10095943 MS.gene041128:CDS 30.0%
! ATATGGTTGATTCTCCAATT+TGG - chr5.2:10095226-10095245 MS.gene041128:intron 30.0%
! ATGTTGTTAGGGATTAGTAT+AGG - chr5.2:10095479-10095498 MS.gene041128:CDS 30.0%
! CAATTTGGAATTGAAATTGC+TGG - chr5.2:10095241-10095260 MS.gene041128:intron 30.0%
! TTGTGATTATGCAGAAGTAT+AGG - chr5.2:10095826-10095845 MS.gene041128:intron 30.0%
!! AAAAGTTGGGTTGATGAAAA+TGG - chr5.2:10094581-10094600 MS.gene041128:intron 30.0%
!! TAATAAGTTAGCCTTGTACA+TGG - chr5.2:10095890-10095909 MS.gene041128:intron 30.0%
!!! AAGAATTTTTTACCGTACAC+GGG - chr5.2:10095565-10095584 MS.gene041128:intron 30.0%
!!! CAAGAATTTTTTACCGTACA+CGG - chr5.2:10095564-10095583 MS.gene041128:intron 30.0%
AATCGATCTTAGTCGTTGTA+TGG - chr5.2:10095332-10095351 MS.gene041128:CDS 35.0%
AATTAGGGAAAAGATGCAAG+TGG - chr5.2:10095648-10095667 MS.gene041128:intron 35.0%
AGCCACTCCTAAACAAATTA+GGG - chr5.2:10095633-10095652 MS.gene041128:intron 35.0%
ATTCCCAGTTTCTTCAATTC+AGG - chr5.2:10095863-10095882 MS.gene041128:intron 35.0%
ATTGTGTTACAGAAGGGTAA+TGG - chr5.2:10094814-10094833 MS.gene041128:intron 35.0%
GATAGGATTGTGTTACAGAA+GGG - chr5.2:10094808-10094827 MS.gene041128:intron 35.0%
GTTGAAGTGCATCAACAAAT+CGG + chr5.2:10095065-10095084 None:intergenic 35.0%
GTTGTTGAGGAAATGAGAAA+CGG + chr5.2:10094299-10094318 None:intergenic 35.0%
TATTGTCACTCACTTGCAAA+TGG + chr5.2:10095702-10095721 None:intergenic 35.0%
TGATAGGATTGTGTTACAGA+AGG - chr5.2:10094807-10094826 MS.gene041128:intron 35.0%
TGATGACTTAGTTCATAGCT+TGG - chr5.2:10094360-10094379 MS.gene041128:CDS 35.0%
! ACAATGAAGGTGATGATGAT+AGG - chr5.2:10094791-10094810 MS.gene041128:intron 35.0%
! AGGAAATGAGAAACGGTTTT+TGG + chr5.2:10094292-10094311 None:intergenic 35.0%
! GGTGCTGTCTTGAATTATTA+TGG - chr5.2:10095161-10095180 MS.gene041128:intron 35.0%
!! GAAATTGCTGGTCTTGTATT+GGG - chr5.2:10095253-10095272 MS.gene041128:intron 35.0%
!! TGAAATTGCTGGTCTTGTAT+TGG - chr5.2:10095252-10095271 MS.gene041128:intron 35.0%
!! TTCCCTAATTTGTTTAGGAG+TGG + chr5.2:10095638-10095657 None:intergenic 35.0%
AAGATGAGCAATCAGATTGC+AGG - chr5.2:10096051-10096070 MS.gene041128:CDS 40.0%
AAGCCATGGAAGAAACAGTA+TGG - chr5.2:10096022-10096041 MS.gene041128:CDS 40.0%
AATCTGGATTACCTGCAGTT+AGG - chr5.2:10095286-10095305 MS.gene041128:CDS 40.0%
ACAACTGAGACAATGACTAC+AGG - chr5.2:10095423-10095442 MS.gene041128:CDS 40.0%
ACAAGCTAAGAGCTTCAGTT+TGG - chr5.2:10094885-10094904 MS.gene041128:intron 40.0%
ACCATCATTAAGGAGGAGTA+TGG + chr5.2:10094434-10094453 None:intergenic 40.0%
ACCTGCAGTTAGGAAATATG+TGG - chr5.2:10095296-10095315 MS.gene041128:CDS 40.0%
AGCAATCAGATTGCAGGAAT+TGG - chr5.2:10096057-10096076 MS.gene041128:CDS 40.0%
AGTCATGGAAATTCCAAGAG+TGG - chr5.2:10094919-10094938 MS.gene041128:intron 40.0%
CAGCCACTCCTAAACAAATT+AGG - chr5.2:10095632-10095651 MS.gene041128:intron 40.0%
CAGGAATTGGAAAAGTGATC+AGG - chr5.2:10096070-10096089 MS.gene041128:CDS 40.0%
CCTCCTGAATTGAAGAAACT+GGG + chr5.2:10095869-10095888 None:intergenic 40.0%
GAATCCCACTCTTGTGTATA+GGG - chr5.2:10095445-10095464 MS.gene041128:CDS 40.0%
GGTATTAACCATGGATGGTT+TGG + chr5.2:10095739-10095758 None:intergenic 40.0%
GTTGATGCACTTCAACAACT+TGG - chr5.2:10095069-10095088 MS.gene041128:intron 40.0%
GTTGTAAGATGCAGATCAGA+TGG + chr5.2:10095408-10095427 None:intergenic 40.0%
TCCACATATTTCCTAACTGC+AGG + chr5.2:10095300-10095319 None:intergenic 40.0%
TCCATACTCCTCCTTAATGA+TGG - chr5.2:10094430-10094449 MS.gene041128:CDS 40.0%
TGATCCCTATACACAAGAGT+GGG + chr5.2:10095452-10095471 None:intergenic 40.0%
TGGTTTGAACAACGTTGTTG+AGG + chr5.2:10094312-10094331 None:intergenic 40.0%
TTGATCCCTATACACAAGAG+TGG + chr5.2:10095453-10095472 None:intergenic 40.0%
TTGATCTTGAGCCATGTACA+AGG + chr5.2:10095904-10095923 None:intergenic 40.0%
! CATCCATACTGTTTCTTCCA+TGG + chr5.2:10096028-10096047 None:intergenic 40.0%
! TAAGGAGGAGTATGGAAAGA+GGG + chr5.2:10094426-10094445 None:intergenic 40.0%
! TTAAGGAGGAGTATGGAAAG+AGG + chr5.2:10094427-10094446 None:intergenic 40.0%
!! AAGTTTCTGAGACATGTCGT+TGG + chr5.2:10094332-10094351 None:intergenic 40.0%
!! AATGGTGGAGGGGTATTTAT+GGG - chr5.2:10094832-10094851 MS.gene041128:intron 40.0%
!! TAATGGTGGAGGGGTATTTA+TGG - chr5.2:10094831-10094850 MS.gene041128:intron 40.0%
!!! AGAAACGGTTTTTGGAACAG+AGG + chr5.2:10094284-10094303 None:intergenic 40.0%
AAGATGCAGATCAGATGGCT+TGG + chr5.2:10095403-10095422 None:intergenic 45.0%
AGAGTTCCAGGAAACAAAGG+AGG - chr5.2:10095016-10095035 MS.gene041128:intron 45.0%
AGCAGAGTTCCAGGAAACAA+AGG - chr5.2:10095013-10095032 MS.gene041128:intron 45.0%
AGGGAAAAGATGCAAGTGGA+TGG - chr5.2:10095652-10095671 MS.gene041128:intron 45.0%
CATGGAAATTCCAAGAGTGG+TGG - chr5.2:10094922-10094941 MS.gene041128:intron 45.0%
CCCAGTTTCTTCAATTCAGG+AGG - chr5.2:10095866-10095885 MS.gene041128:intron 45.0%
CTCAAACTCCAAACCATCCA+TGG - chr5.2:10095728-10095747 MS.gene041128:CDS 45.0%
GATGCACTTCAACAACTTGG+TGG - chr5.2:10095072-10095091 MS.gene041128:intron 45.0%
GCCTCCTGAATTGAAGAAAC+TGG + chr5.2:10095870-10095889 None:intergenic 45.0%
GGAATCCCACTCTTGTGTAT+AGG - chr5.2:10095444-10095463 MS.gene041128:CDS 45.0%
! GTGTTACAGAAGGGTAATGG+TGG - chr5.2:10094817-10094836 MS.gene041128:intron 45.0%
! TACAGAAGGGTAATGGTGGA+GGG - chr5.2:10094821-10094840 MS.gene041128:intron 45.0%
! TCAGAGTCTGTTTCTCCACA+GGG - chr5.2:10095954-10095973 MS.gene041128:CDS 45.0%
! TTACAGAAGGGTAATGGTGG+AGG - chr5.2:10094820-10094839 MS.gene041128:intron 45.0%
! TTGGTGTCTGAAGTGAGTCA+TGG - chr5.2:10094904-10094923 MS.gene041128:intron 45.0%
!! ATTGCTGGTCTTGTATTGGG+TGG - chr5.2:10095256-10095275 MS.gene041128:intron 45.0%
!! TTGCTGGTCTTGTATTGGGT+GGG - chr5.2:10095257-10095276 MS.gene041128:CDS 45.0%
!!! ATAAAATGTTTTTTTTTTTT+TGG - chr5.2:10094764-10094783 MS.gene041128:intron 5.0%
AACCTTTGCAGCAGAGTTCC+AGG - chr5.2:10095004-10095023 MS.gene041128:intron 50.0%
ACAGATCCACCAAGGAGAAG+AGG + chr5.2:10095990-10096009 None:intergenic 50.0%
AGAACTTCCACCACCACTCT+TGG + chr5.2:10094935-10094954 None:intergenic 50.0%
CCAAGGAGAAGAGGAGTAAG+AGG + chr5.2:10095981-10096000 None:intergenic 50.0%
CCTCTTACTCCTCTTCTCCT+TGG - chr5.2:10095978-10095997 MS.gene041128:CDS 50.0%
CTTACTCCTCTTCTCCTTGG+TGG - chr5.2:10095981-10096000 MS.gene041128:CDS 50.0%
GAACTCTGCTGCAAAGGTTG+CGG + chr5.2:10095003-10095022 None:intergenic 50.0%
GACCTTGAACAGATCCACCA+AGG + chr5.2:10095998-10096017 None:intergenic 50.0%
GGAAATTCCAAGAGTGGTGG+TGG - chr5.2:10094925-10094944 MS.gene041128:intron 50.0%
GGATGAGTTCAGCTCAGCTA+TGG - chr5.2:10094640-10094659 MS.gene041128:intron 50.0%
GTTCAAGGTCTCTCAAGCCA+TGG - chr5.2:10096008-10096027 MS.gene041128:CDS 50.0%
TTCCTGGAACTCTGCTGCAA+AGG + chr5.2:10095009-10095028 None:intergenic 50.0%
! ACAGAAGGGTAATGGTGGAG+GGG - chr5.2:10094822-10094841 MS.gene041128:intron 50.0%
! CAACAACTTGGTGGAGCACA+TGG - chr5.2:10095081-10095100 MS.gene041128:intron 50.0%
! CTCAGAGTCTGTTTCTCCAC+AGG - chr5.2:10095953-10095972 MS.gene041128:CDS 50.0%
! CTCCTTGGTGGATCTGTTCA+AGG - chr5.2:10095993-10096012 MS.gene041128:CDS 50.0%
! TACCGTACACGGGCTATGTA+GGG - chr5.2:10095575-10095594 MS.gene041128:intron 50.0%
! TTACCGTACACGGGCTATGT+AGG - chr5.2:10095574-10095593 MS.gene041128:intron 50.0%
!! AAGAGTGGTGGTGGAAGTTC+TGG - chr5.2:10094934-10094953 MS.gene041128:intron 50.0%
!! TGCTGGTCTTGTATTGGGTG+GGG - chr5.2:10095258-10095277 MS.gene041128:CDS 50.0%
AGAGGAGTAAGAGGACCCTG+TGG + chr5.2:10095972-10095991 None:intergenic 55.0%
AGGGTGCGTATGAGCCTAAC+TGG - chr5.2:10095594-10095613 MS.gene041128:intron 55.0%
CACCCTACATAGCCCGTGTA+CGG + chr5.2:10095580-10095599 None:intergenic 55.0%
! CCAGCACCTCCTTTGTTTCC+TGG + chr5.2:10095025-10095044 None:intergenic 55.0%
! CCAGGAAACAAAGGAGGTGC+TGG - chr5.2:10095022-10095041 MS.gene041128:intron 55.0%
!! ACAAAGGAGGTGCTGGTCGT+CGG - chr5.2:10095029-10095048 MS.gene041128:intron 55.0%
!! CTGGTCTTGTATTGGGTGGG+GGG - chr5.2:10095260-10095279 MS.gene041128:CDS 55.0%
!! GCTGGTCTTGTATTGGGTGG+GGG - chr5.2:10095259-10095278 MS.gene041128:CDS 55.0%
ATTGGGTGGGGGGAGCAATC+TGG - chr5.2:10095270-10095289 MS.gene041128:CDS 60.0%
CTCTGCTGCAAAGGTTGCGG+CGG + chr5.2:10095000-10095019 None:intergenic 60.0%
TGCAAAGGTTGCGGCGGCTG+AGG + chr5.2:10094994-10095013 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 10094244 10096115 10094244 ID=MS.gene041128
chr5.2 mRNA 10094244 10096115 10094244 ID=MS.gene041128.t1;Parent=MS.gene041128
chr5.2 exon 10095908 10096115 10095908 ID=MS.gene041128.t1.exon1;Parent=MS.gene041128.t1
chr5.2 CDS 10095908 10096115 10095908 ID=cds.MS.gene041128.t1;Parent=MS.gene041128.t1
chr5.2 exon 10095679 10095806 10095679 ID=MS.gene041128.t1.exon2;Parent=MS.gene041128.t1
chr5.2 CDS 10095679 10095806 10095679 ID=cds.MS.gene041128.t1;Parent=MS.gene041128.t1
chr5.2 exon 10095257 10095569 10095257 ID=MS.gene041128.t1.exon3;Parent=MS.gene041128.t1
chr5.2 CDS 10095257 10095569 10095257 ID=cds.MS.gene041128.t1;Parent=MS.gene041128.t1
chr5.2 exon 10094651 10094727 10094651 ID=MS.gene041128.t1.exon4;Parent=MS.gene041128.t1
chr5.2 CDS 10094651 10094727 10094651 ID=cds.MS.gene041128.t1;Parent=MS.gene041128.t1
chr5.2 exon 10094244 10094519 10094244 ID=MS.gene041128.t1.exon5;Parent=MS.gene041128.t1
chr5.2 CDS 10094244 10094519 10094244 ID=cds.MS.gene041128.t1;Parent=MS.gene041128.t1
Gene Sequence

>MS.gene041128

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCATTTCCTCAACAACGTTGTTCAAACCAACGACATGTCTCAGAAACTTTCAATGCTTGATGACTTAGTTCATAGCTTGGAAGAAGAAATGAAGAAAGTTCTTGCTTTCAAACGTGAACTCCCTCTTTCCATACTCCTCCTTAATGATGGTAACTAATTTTCTAATTAATTTTTGTGGTATTTCTTGTTTTTTGGATTTAACCTTTTTCTTTTTTTGTTAATTTTTTATTTTTGTTGTTAATTGATGTAGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGATGATTTGAAAGAAAACTGTGATAATAAAAAGAATTGGATGAGTTCAGCTCAGCTATGGACTAATCAAACAAAATCGGTAATTTTAATATTTTATAAATTATCAAATTACCCTTTTCTTTTAATTGTGATTTTTATTTATTGAAGTGAGATTTGAGATAGATAAAATGTTTTTTTTTTTTTGGTAGAACAATGAAGGTGATGATGATAGGATTGTGTTACAGAAGGGTAATGGTGGAGGGGTATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTCAGTTTGGTGTCTGAAGTGAGTCATGGAAATTCCAAGAGTGGTGGTGGAAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCGCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCGGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAACTTGGTGGAGCACATGGTAAATTGTTTGTGTTCAGACTTCAGATTGTTTTTATTGTTAATTAGTTGTGTTATGCGGTGCTGTCTTGAATTATTATGGTGTAAGAATTTTCTTAGTCTCTATTTTATTTTAGATTTGGGAATATGGTTGATTCTCCAATTTGGAATTGAAATTGCTGGTCTTGTATTGGGTGGGGGGAGCAATCTGGATTACCTGCAGTTAGGAAATATGTGGATTTAGTGTATTTAATCGATCTTAGTCGTTGTATGGATATCAAACGATTTTATAGTAGTGTAAAATAGTTCAGCTTGAAATCCAAGCCATCTGATCTGCATCTTACAACTGAGACAATGACTACAGGAATCCCACTCTTGTGTATAGGGATCAAGATTACATGTTGTTAGGGATTAGTATAGGCACATATTTGTTATAACTTAGAACTGTTCGATTAAGATTGAATGTTTTCATCTTGCTGATTGCAAGAATTTTTTACCGTACACGGGCTATGTAGGGTGCGTATGAGCCTAACTGGTTTTCTTTTTTGTAGCAGCCACTCCTAAACAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAGTGAGTGACAATATTTCATCTCAAACTCCAAACCATCCATGGTTAATACCATTATTAGTTTCTTTGTATGAATTTGATTCTTTTGTTATGTCATGTCAAGCAATTTATGTAGTACATTTGTGATTATGCAGAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCTTCAATTCAGGAGGCTAATAAGTTAGCCTTGTACATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTCTCAAGCCATGGAAGAAACAGTATGGATGCAGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA
Protein sequence

>MS.gene041128.t1

MEQQQLNLDLTLASVPKTVSHFLNNVVQTNDMSQKLSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMDDLKENCDNKKNWMSSAQLWTNQTKSNNEGDDDRIVLQKGNGGGVFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGGGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHAATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE