AlfalfaGEDB | MS.gene041358

M. sativa cultivar XinJiangDaYe / MS.gene041358

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041358.t1	XP_019428473.1	74.5	165	42	0	1	165	1	165	2.80E-63	251.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041358.t1	Q9FJK3	54.6	163	69	2	1	161	1	160	1.6e-43	177.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041358.t1	A0A1S2Y776	78.7	155	33	0	1	155	1	155	1.3e-62	248.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene041358.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041358.t1	MTR_8g036130	73.410	173	43	2	1	171	1	172	6.21e-85	250
MS.gene041358.t1	MTR_2g035610	94.262	122	7	0	1	122	1	122	9.31e-76	222
MS.gene041358.t1	MTR_4g031910	68.421	171	53	1	1	171	1	170	7.18e-74	221
MS.gene041358.t1	MTR_3g065100	65.625	160	54	1	1	160	1	159	1.07e-70	214
MS.gene041358.t1	MTR_4g032620	64.198	162	58	0	1	162	1	162	1.29e-70	213
MS.gene041358.t1	MTR_3g466980	62.821	156	57	1	1	156	1	155	1.78e-70	213
MS.gene041358.t1	MTR_4g032260	70.323	155	46	0	1	155	1	155	1.08e-64	196
MS.gene041358.t1	MTR_4g032290	64.912	171	59	1	1	171	1	170	1.28e-64	198
MS.gene041358.t1	MTR_3g466830	50.314	159	78	1	3	161	2	159	3.21e-55	172
MS.gene041358.t1	MTR_4g063790	45.223	157	86	0	1	157	1	157	2.81e-49	157
MS.gene041358.t1	MTR_3g466890	44.937	158	86	1	3	160	2	158	7.45e-47	150
MS.gene041358.t1	MTR_3g467080	42.581	155	89	0	1	155	1	155	8.23e-44	142
MS.gene041358.t1	MTR_5g075380	41.875	160	92	1	1	160	1	159	8.62e-43	140
MS.gene041358.t1	MTR_3g031100	42.675	157	90	0	1	157	1	157	2.62e-42	141
MS.gene041358.t1	MTR_2g016210	41.772	158	92	0	1	158	1	158	3.93e-42	141
MS.gene041358.t1	MTR_2g035590	80.612	98	19	0	74	171	1	98	6.16e-42	136
MS.gene041358.t1	MTR_5g047580	42.581	155	87	2	1	155	1	153	3.38e-40	133
MS.gene041358.t1	MTR_1g090697	39.241	158	94	2	1	158	1	156	4.14e-38	128
MS.gene041358.t1	MTR_7g011950	43.506	154	85	2	1	154	1	152	9.66e-36	122
MS.gene041358.t1	MTR_3g466900	41.085	129	75	1	32	160	2	129	2.02e-35	120
MS.gene041358.t1	MTR_1g077390	39.610	154	91	2	1	154	1	152	5.77e-35	120
MS.gene041358.t1	MTR_3g031240	34.177	158	104	0	1	158	1	158	6.60e-35	120
MS.gene041358.t1	MTR_1g090710	35.443	158	101	1	1	158	1	157	2.05e-34	119
MS.gene041358.t1	MTR_1g090783	37.179	156	96	2	3	158	2	155	2.23e-34	119
MS.gene041358.t1	MTR_3g465410	41.860	129	75	0	29	157	1	129	2.37e-34	118
MS.gene041358.t1	MTR_3g466930	41.860	129	74	1	32	160	2	129	2.46e-34	117
MS.gene041358.t1	MTR_1g077320	42.857	154	86	2	1	154	1	152	3.54e-34	118
MS.gene041358.t1	MTR_1g084950	40.645	155	90	2	1	155	1	153	4.69e-33	115
MS.gene041358.t1	MTR_7g055800	53.782	119	27	2	1	119	1	91	4.79e-33	113
MS.gene041358.t1	MTR_1g077300	42.857	154	84	2	1	154	1	150	5.32e-33	115
MS.gene041358.t1	MTR_4g028720	40.909	154	89	2	1	154	1	152	7.14e-33	115
MS.gene041358.t1	MTR_7g055790	58.559	111	28	1	1	111	59	151	2.38e-30	108
MS.gene041358.t1	MTR_4g028800	70.833	72	19	1	1	72	1	70	1.22e-29	103
MS.gene041358.t1	MTR_2g035580	36.774	155	97	1	1	155	1	154	3.42e-27	100
MS.gene041358.t1	MTR_7g055940	51.887	106	23	2	1	106	1	78	1.89e-25	93.6
MS.gene041358.t1	MTR_5g047560	32.903	155	86	3	1	155	1	137	2.00e-22	87.8
MS.gene041358.t1	MTR_4g019670	37.121	132	80	1	1	129	1	132	2.57e-22	92.8
MS.gene041358.t1	MTR_3g093900	32.168	143	97	0	3	145	6	148	1.44e-21	90.9
MS.gene041358.t1	MTR_7g106510	36.364	132	81	1	1	129	1	132	5.37e-21	89.4
MS.gene041358.t1	MTR_4g084780	61.538	52	20	0	74	125	1	52	6.57e-15	68.6
MS.gene041358.t1	MTR_6g018920	31.250	112	70	3	1	106	1	111	8.85e-13	65.5
MS.gene041358.t1	MTR_1g114730	26.619	139	85	5	1	127	1	134	7.11e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041358.t1	AT5G48670	54.321	162	71	2	1	161	1	160	1.03e-55	178
MS.gene041358.t1	AT5G26630	47.904	167	86	1	1	167	1	166	4.66e-46	150
MS.gene041358.t1	AT1G65300	42.424	165	94	1	3	167	2	165	7.11e-41	139
MS.gene041358.t1	AT1G65330	42.593	162	92	1	3	164	2	162	1.57e-38	133
MS.gene041358.t1	AT5G26650	41.772	158	91	1	4	161	2	158	5.29e-38	134
MS.gene041358.t1	AT5G27960	41.772	158	91	1	4	161	2	158	7.04e-38	132
MS.gene041358.t1	AT1G31630	35.849	159	100	2	3	161	2	158	2.00e-34	124
MS.gene041358.t1	AT3G05860	39.024	164	95	2	1	162	1	161	2.70e-33	117
MS.gene041358.t1	AT5G27810	52.688	93	44	0	29	121	1	93	5.78e-33	114
MS.gene041358.t1	AT3G05860	39.024	164	95	2	1	162	1	161	1.61e-32	116
MS.gene041358.t1	AT3G05860	39.024	164	95	2	1	162	1	161	2.38e-32	116
MS.gene041358.t1	AT1G31640	38.411	151	91	2	3	153	2	150	7.31e-31	116
MS.gene041358.t1	AT2G28700	36.184	152	92	2	1	150	1	149	1.54e-27	105
MS.gene041358.t1	AT5G26580	34.783	161	90	2	1	161	1	146	3.30e-26	102
MS.gene041358.t1	AT1G22590	30.061	163	109	2	1	163	1	158	2.67e-21	85.5
MS.gene041358.t1	AT5G06500	31.818	154	103	1	1	154	1	152	5.14e-20	84.0
MS.gene041358.t1	AT2G40210	37.795	127	79	0	1	127	1	127	3.67e-19	83.6
MS.gene041358.t1	AT5G55690	39.362	94	50	2	1	87	1	94	2.68e-13	66.2
MS.gene041358.t1	AT5G55690	39.362	94	50	2	1	87	1	94	2.68e-13	66.2
MS.gene041358.t1	AT5G58890	40.179	112	58	4	1	105	1	110	1.57e-12	64.3
MS.gene041358.t1	AT2G15660	24.516	155	99	3	11	163	18	156	7.28e-12	62.0

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATCAATATCCTTCAAATTT+TGG	0.165838	2.1:+55381961	None:intergenic
TTCTTGTTCTTCTCTAATTC+AGG	0.242401	2.1:+55382170	None:intergenic
ATGAACCTCAACCTCATATT+TGG	0.258532	2.1:-55382241	MS.gene041358:CDS
CACAATCATGACTAGGAAAA+AGG	0.298378	2.1:-55382399	None:intergenic
CATCACCATGGGGAGTTCAT+AGG	0.334299	2.1:-55382217	MS.gene041358:CDS
TGAAGCATGTGCTATAGTTC+AGG	0.354873	2.1:-55382270	MS.gene041358:CDS
GAAGGATATTGATAGAAGAT+TGG	0.427774	2.1:-55381952	MS.gene041358:CDS
GTTGATTGACCAAAATTTGA+AGG	0.429205	2.1:-55381970	MS.gene041358:CDS
ATAGGGTGCTCTCAAAATTC+AGG	0.434987	2.1:-55382199	MS.gene041358:CDS
TTTGCGTCATGAATGTCTCT+TGG	0.452946	2.1:+55382138	None:intergenic
TTGGAAGAAGTGGATAATAA+TGG	0.455688	2.1:-55381933	MS.gene041358:CDS
GGAAATCAACCTATAAGAAA+AGG	0.476350	2.1:-55382343	MS.gene041358:CDS
GATGAACTAAGCACGCTTTG+TGG	0.477096	2.1:-55382296	MS.gene041358:CDS
CTCATATTTGGCCATCACCA+TGG	0.499740	2.1:-55382229	MS.gene041358:CDS
TTGATTTGAATGATCTTGCT+TGG	0.502629	2.1:-55381992	MS.gene041358:CDS
TCATATTTGGCCATCACCAT+GGG	0.503042	2.1:-55382228	MS.gene041358:CDS
ATCACCATGGGGAGTTCATA+GGG	0.514521	2.1:-55382216	MS.gene041358:CDS
GAAGCATGTGCTATAGTTCA+GGG	0.519428	2.1:-55382269	MS.gene041358:CDS
TGATAGAAGATTGGAAGAAG+TGG	0.523326	2.1:-55381943	MS.gene041358:CDS
CATGGTGATGGCCAAATATG+AGG	0.531321	2.1:+55382230	None:intergenic
AGAGACATTCATGACGCAAA+GGG	0.534224	2.1:-55382135	MS.gene041358:CDS
TGAGCAGTCATGACTTGACT+TGG	0.542884	2.1:+55381906	None:intergenic
TCATTGTCAATGACGCTGCT+CGG	0.556837	2.1:-55382364	MS.gene041358:CDS
CTATGAACTCCCCATGGTGA+TGG	0.559546	2.1:+55382218	None:intergenic
AGCAGCGTCATTGACAATGA+AGG	0.575999	2.1:+55382367	None:intergenic
CACAACTCACAATCATGACT+AGG	0.590575	2.1:-55382406	None:intergenic
AAGAGACATTCATGACGCAA+AGG	0.614539	2.1:-55382136	MS.gene041358:CDS
GATGGCCAAATATGAGGTTG+AGG	0.620009	2.1:+55382236	None:intergenic
AAAACTAAGGAAGGGAAACA+GGG	0.623030	2.1:-55382087	MS.gene041358:CDS
ATATGAGGTTGAGGTTCATG+AGG	0.635051	2.1:+55382245	None:intergenic
AGCACCCTATGAACTCCCCA+TGG	0.654106	2.1:+55382212	None:intergenic
CATATTTGGCCATCACCATG+GGG	0.723900	2.1:-55382227	MS.gene041358:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTATTCATCCTTTTCTTAT+AGG	+	chr2.1:55381960-55381979	None:intergenic	20.0%
!!!	TATCAATATCCTTCAAATTT+TGG	+	chr2.1:55382333-55382352	None:intergenic	20.0%
!	AAAGTGAAAAAACTAAGGAA+GGG	-	chr2.1:55382196-55382215	MS.gene041358:CDS	25.0%
	AGGAGAAAGTGAAAAAACTA+AGG	-	chr2.1:55382191-55382210	MS.gene041358:CDS	30.0%
	GAAAGTGAAAAAACTAAGGA+AGG	-	chr2.1:55382195-55382214	MS.gene041358:CDS	30.0%
	GAAGGATATTGATAGAAGAT+TGG	-	chr2.1:55382339-55382358	MS.gene041358:CDS	30.0%
	GGAAATCAACCTATAAGAAA+AGG	-	chr2.1:55381948-55381967	MS.gene041358:CDS	30.0%
	GTTGATTGACCAAAATTTGA+AGG	-	chr2.1:55382321-55382340	MS.gene041358:CDS	30.0%
	TTCTTGTTCTTCTCTAATTC+AGG	+	chr2.1:55382124-55382143	None:intergenic	30.0%
	TTGGAAGAAGTGGATAATAA+TGG	-	chr2.1:55382358-55382377	MS.gene041358:CDS	30.0%
!	TTGATTTGAATGATCTTGCT+TGG	-	chr2.1:55382299-55382318	MS.gene041358:CDS	30.0%
	AAAAACTAAGGAAGGGAAAC+AGG	-	chr2.1:55382203-55382222	MS.gene041358:CDS	35.0%
	AAAACTAAGGAAGGGAAACA+GGG	-	chr2.1:55382204-55382223	MS.gene041358:CDS	35.0%
	ATGAACCTCAACCTCATATT+TGG	-	chr2.1:55382050-55382069	MS.gene041358:CDS	35.0%
	TGATAGAAGATTGGAAGAAG+TGG	-	chr2.1:55382348-55382367	MS.gene041358:CDS	35.0%
	AAGAGACATTCATGACGCAA+AGG	-	chr2.1:55382155-55382174	MS.gene041358:CDS	40.0%
	AGAGACATTCATGACGCAAA+GGG	-	chr2.1:55382156-55382175	MS.gene041358:CDS	40.0%
	ATATGAGGTTGAGGTTCATG+AGG	+	chr2.1:55382049-55382068	None:intergenic	40.0%
	GAAGCATGTGCTATAGTTCA+GGG	-	chr2.1:55382022-55382041	MS.gene041358:CDS	40.0%
	TCATATTTGGCCATCACCAT+GGG	-	chr2.1:55382063-55382082	MS.gene041358:CDS	40.0%
	TGAAGCATGTGCTATAGTTC+AGG	-	chr2.1:55382021-55382040	MS.gene041358:CDS	40.0%
	TTTGCGTCATGAATGTCTCT+TGG	+	chr2.1:55382156-55382175	None:intergenic	40.0%
!	ATAGGGTGCTCTCAAAATTC+AGG	-	chr2.1:55382092-55382111	MS.gene041358:CDS	40.0%
	AGCAGCGTCATTGACAATGA+AGG	+	chr2.1:55381927-55381946	None:intergenic	45.0%
	ATCACCATGGGGAGTTCATA+GGG	-	chr2.1:55382075-55382094	MS.gene041358:CDS	45.0%
	CATATTTGGCCATCACCATG+GGG	-	chr2.1:55382064-55382083	MS.gene041358:CDS	45.0%
	CTCATATTTGGCCATCACCA+TGG	-	chr2.1:55382062-55382081	MS.gene041358:CDS	45.0%
	GATGAACTAAGCACGCTTTG+TGG	-	chr2.1:55381995-55382014	MS.gene041358:CDS	45.0%
	GATGGCCAAATATGAGGTTG+AGG	+	chr2.1:55382058-55382077	None:intergenic	45.0%
	TCATTGTCAATGACGCTGCT+CGG	-	chr2.1:55381927-55381946	MS.gene041358:CDS	45.0%
	TGAGCAGTCATGACTTGACT+TGG	+	chr2.1:55382388-55382407	None:intergenic	45.0%
!!	CATGACGCAAAGGGTTTTGA+AGG	-	chr2.1:55382165-55382184	MS.gene041358:CDS	45.0%
!!	CATGGTGATGGCCAAATATG+AGG	+	chr2.1:55382064-55382083	None:intergenic	45.0%
!!!	GCAAAGGGTTTTGAAGGCTA+AGG	-	chr2.1:55382171-55382190	MS.gene041358:CDS	45.0%
	CATCACCATGGGGAGTTCAT+AGG	-	chr2.1:55382074-55382093	MS.gene041358:CDS	50.0%
	CTATGAACTCCCCATGGTGA+TGG	+	chr2.1:55382076-55382095	None:intergenic	50.0%
	AGCACCCTATGAACTCCCCA+TGG	+	chr2.1:55382082-55382101	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	55381899	55382414	55381899	ID=MS.gene041358
chr2.1	mRNA	55381899	55382414	55381899	ID=MS.gene041358.t1;Parent=MS.gene041358
chr2.1	exon	55381899	55382414	55381899	ID=MS.gene041358.t1.exon1;Parent=MS.gene041358.t1
chr2.1	CDS	55381899	55382414	55381899	ID=cds.MS.gene041358.t1;Parent=MS.gene041358.t1

Gene Sequence

>MS.gene041358

ATGACTAGGAAAAAGGTGAAACTCGCCTTCATTGTCAATGACGCTGCTCGGAAATCAACCTATAAGAAAAGGATGAATAATTTATTGAAGAAAGTCGATGAACTAAGCACGCTTTGTGGTATTGAAGCATGTGCTATAGTTCAGGGTCCTCATGAACCTCAACCTCATATTTGGCCATCACCATGGGGAGTTCATAGGGTGCTCTCAAAATTCAGGACTATGCCTGAATTAGAGAAGAACAAGAAAATGATGAACCAAGAGACATTCATGACGCAAAGGGTTTTGAAGGCTAAGGAGAAAGTGAAAAAACTAAGGAAGGGAAACAGGGAGCAAGAGATGACAATGATCATGTTTCAATGTCTTAATGCTGAAAAAATTGTGCACGATGATATGTCAATGATTGATTTGAATGATCTTGCTTGGTTGATTGACCAAAATTTGAAGGATATTGATAGAAGATTGGAAGAAGTGGATAATAATGGTCATCCAAGTCAAGTCATGACTGCTCAAAGTTAA

Protein sequence

>MS.gene041358.t1

MTRKKVKLAFIVNDAARKSTYKKRMNNLLKKVDELSTLCGIEACAIVQGPHEPQPHIWPSPWGVHRVLSKFRTMPELEKNKKMMNQETFMTQRVLKAKEKVKKLRKGNREQEMTMIMFQCLNAEKIVHDDMSMIDLNDLAWLIDQNLKDIDRRLEEVDNNGHPSQVMTAQS