AlfalfaGEDB | MS.gene041359

M. sativa cultivar XinJiangDaYe / MS.gene041359

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041359.t1	XP_003594864.1	87.3	158	20	0	1	158	1	158	9.70E-71	276.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041359.t1	Q9FJK3	39.6	154	93	0	1	154	1	154	6.9e-25	115.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041359.t1	G7IND2	87.3	158	20	0	1	158	1	158	7.0e-71	276.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene041359.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041359.t1	MTR_2g035580	91.139	158	14	0	1	158	1	158	2.63e-93	267
MS.gene041359.t1	MTR_3g466980	46.203	158	85	0	1	158	1	158	2.70e-46	150
MS.gene041359.t1	MTR_3g467080	49.351	154	77	1	1	153	1	154	5.89e-46	147
MS.gene041359.t1	MTR_4g063790	46.753	154	81	1	1	153	1	154	5.33e-43	140
MS.gene041359.t1	MTR_3g065100	44.156	154	86	0	1	154	1	154	5.25e-38	130
MS.gene041359.t1	MTR_3g031100	42.138	159	91	1	1	158	1	159	7.59e-37	127
MS.gene041359.t1	MTR_4g032620	37.662	154	95	1	1	153	1	154	8.58e-35	121
MS.gene041359.t1	MTR_3g465410	47.541	122	63	1	30	150	2	123	1.36e-33	115
MS.gene041359.t1	MTR_2g016210	38.750	160	93	2	1	157	1	158	6.85e-33	117
MS.gene041359.t1	MTR_3g466830	39.597	149	90	0	4	152	3	151	8.01e-33	114
MS.gene041359.t1	MTR_3g031240	38.462	156	95	1	1	155	1	156	5.24e-31	110
MS.gene041359.t1	MTR_5g075380	33.333	153	102	0	1	153	1	153	1.46e-30	108
MS.gene041359.t1	MTR_4g031910	37.179	156	97	1	1	155	1	156	2.69e-29	107
MS.gene041359.t1	MTR_8g036130	37.975	158	95	2	1	155	1	158	3.05e-29	107
MS.gene041359.t1	MTR_1g090783	33.333	153	101	1	6	158	5	156	1.79e-28	103
MS.gene041359.t1	MTR_5g047580	34.194	155	101	1	1	155	1	154	4.95e-28	102
MS.gene041359.t1	MTR_4g032260	36.943	157	98	1	1	156	1	157	2.64e-27	100
MS.gene041359.t1	MTR_1g077390	33.987	153	100	1	1	153	1	152	5.48e-27	99.4
MS.gene041359.t1	MTR_3g466890	35.417	144	93	0	6	149	5	148	2.82e-26	97.8
MS.gene041359.t1	MTR_1g090697	30.968	155	106	1	1	155	1	154	1.18e-23	90.9
MS.gene041359.t1	MTR_2g035610	36.752	117	74	0	1	117	1	117	2.34e-23	89.0
MS.gene041359.t1	MTR_4g032290	35.032	157	101	1	1	156	1	157	1.08e-22	90.1
MS.gene041359.t1	MTR_4g028720	35.897	156	97	3	1	155	1	154	5.85e-22	86.7
MS.gene041359.t1	MTR_3g466930	36.885	122	77	0	32	153	2	123	1.05e-21	85.1
MS.gene041359.t1	MTR_1g090710	31.210	157	104	3	1	155	1	155	1.18e-21	85.9
MS.gene041359.t1	MTR_7g011950	32.903	155	103	1	1	155	1	154	1.60e-21	85.5
MS.gene041359.t1	MTR_1g084950	34.194	155	101	1	1	155	1	154	2.90e-21	84.7
MS.gene041359.t1	MTR_3g466900	35.246	122	79	0	32	153	2	123	1.01e-20	82.8
MS.gene041359.t1	MTR_1g077320	30.323	155	107	1	1	155	1	154	6.20e-20	81.3
MS.gene041359.t1	MTR_1g077300	36.129	155	92	4	1	153	1	150	5.37e-18	76.3
MS.gene041359.t1	MTR_4g028800	41.096	73	41	1	1	73	1	71	7.90e-18	73.6
MS.gene041359.t1	MTR_5g047560	25.806	155	98	2	1	155	1	138	1.13e-13	64.7
MS.gene041359.t1	MTR_4g019670	28.814	118	80	1	11	128	14	127	5.26e-11	60.1
MS.gene041359.t1	MTR_7g106510	27.273	121	78	2	11	128	14	127	6.76e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041359.t1	AT5G48670	41.667	156	87	2	1	154	1	154	6.84e-32	116
MS.gene041359.t1	AT3G05860	36.913	149	94	0	1	149	1	149	2.48e-29	107
MS.gene041359.t1	AT1G65300	37.086	151	95	0	4	154	3	153	3.03e-29	108
MS.gene041359.t1	AT1G65330	39.333	150	89	2	6	154	5	153	3.43e-29	108
MS.gene041359.t1	AT3G05860	39.103	156	81	3	1	149	1	149	1.32e-28	106
MS.gene041359.t1	AT3G05860	39.103	156	81	3	1	149	1	149	1.47e-28	106
MS.gene041359.t1	AT1G31630	33.333	153	91	3	6	153	5	151	6.79e-24	95.5
MS.gene041359.t1	AT1G31640	35.915	142	90	1	11	152	10	150	1.98e-23	95.5
MS.gene041359.t1	AT5G27810	43.182	88	50	0	30	117	2	89	7.43e-23	87.8
MS.gene041359.t1	AT5G26650	32.237	152	103	0	4	155	2	153	1.68e-22	92.0
MS.gene041359.t1	AT5G27960	31.579	152	104	0	4	155	2	153	1.35e-21	89.0
MS.gene041359.t1	AT1G22590	36.111	108	69	0	11	118	11	118	5.07e-21	84.3
MS.gene041359.t1	AT2G28700	32.680	153	95	3	1	149	1	149	5.85e-20	84.7
MS.gene041359.t1	AT2G40210	32.479	117	79	0	1	117	1	117	9.34e-15	70.5
MS.gene041359.t1	AT5G26580	28.571	154	96	3	1	154	1	140	2.89e-14	69.3
MS.gene041359.t1	AT5G26630	26.623	154	113	0	1	154	1	154	3.26e-14	67.8
MS.gene041359.t1	AT5G06500	28.182	110	75	1	1	110	1	106	4.28e-13	65.1

Find 27 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGATCTATCATTGCTTAT+TGG	0.201999	2.1:-55373676	MS.gene041359:CDS
CTTCTCTTTCGATTTCATTA+TGG	0.221424	2.1:+55373804	None:intergenic
ATTCATACGGGTGAATTTGA+TGG	0.333961	2.1:-55373721	MS.gene041359:CDS
AGATGGTGTTGGACAAGTTT+AGG	0.350542	2.1:-55373888	MS.gene041359:CDS
GTTTAGGAGCTTTCCTGAAT+CGG	0.417309	2.1:-55373872	MS.gene041359:CDS
TAAACTTGTCCAACACCATC+TGG	0.418271	2.1:+55373890	None:intergenic
AGTGAAATCACCACACTTTG+TGG	0.451943	2.1:-55373985	MS.gene041359:CDS
GGAAGACAACATTCAACAAA+AGG	0.464060	2.1:-55374417	MS.gene041359:CDS
ATCTATGATGAAAACAATGA+TGG	0.489972	2.1:-55373943	MS.gene041359:CDS
ACTTGGTGAATTCATTCATA+CGG	0.496749	2.1:-55373734	MS.gene041359:CDS
AGAGAAGTTGAAGAAACAAA+AGG	0.500916	2.1:-55373788	MS.gene041359:CDS
CCTGAATCGGAACAAAGAAA+AGG	0.516491	2.1:-55373859	MS.gene041359:CDS
GGAACAAAGAAAAGGAAAGC+TGG	0.520124	2.1:-55373851	MS.gene041359:CDS
AGTTGAAGAAACAAAAGGAC+AGG	0.532305	2.1:-55373783	MS.gene041359:CDS
AAAAGGAAAGCTGGATCATG+AGG	0.532487	2.1:-55373842	MS.gene041359:CDS
CTTGGTGAATTCATTCATAC+GGG	0.535817	2.1:-55373733	MS.gene041359:CDS
AAGAAAATGAATGATCTACT+TGG	0.551967	2.1:-55373751	MS.gene041359:CDS
ACGGTTTATCCATCAAAAGA+AGG	0.566324	2.1:-55373916	MS.gene041359:CDS
ACATAACTGATGACTTGAAG+AGG	0.573872	2.1:-55374438	MS.gene041359:CDS
AGAAGGAGTCCAGATGGTGT+TGG	0.590630	2.1:-55373899	MS.gene041359:CDS
ACCGAAAAGAAGTCGATGAG+AGG	0.615793	2.1:-55373648	MS.gene041359:CDS
ATCAAAAGAAGGAGTCCAGA+TGG	0.615817	2.1:-55373905	MS.gene041359:CDS
TGCATCAATTCCACAAAGTG+TGG	0.616904	2.1:+55373975	None:intergenic
TGAAAACAATGATGGAGCAA+CGG	0.627126	2.1:-55373935	MS.gene041359:CDS
ACATTCAACAAAAGGAAGCA+TGG	0.630577	2.1:-55374409	MS.gene041359:intron
AAAGGAAAGCTGGATCATGA+GGG	0.661580	2.1:-55373841	MS.gene041359:CDS
CTTTGTGGAATTGATGCATG+TGG	0.684212	2.1:-55373970	MS.gene041359:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAAATAACAATAATTTCAA+AGG	+	chr2.1:55373977-55373996	None:intergenic	15.0%
!!	ACATAACTGATATGTTATTA+TGG	+	chr2.1:55373951-55373970	None:intergenic	20.0%
!!	AGTGTTCTTTATAGAAATAA+AGG	+	chr2.1:55373727-55373746	None:intergenic	20.0%
!!	TCATATTGATAAATTCCTAA+TGG	+	chr2.1:55373851-55373870	None:intergenic	20.0%
!!!	ACAATATTTTAAGATCATGT+CGG	-	chr2.1:55373997-55374016	MS.gene041359:CDS	20.0%
!!!	TTTCATATTTTTAGTATCCA+TGG	+	chr2.1:55373815-55373834	None:intergenic	20.0%
!	AAGAAAATGAATGATCTACT+TGG	-	chr2.1:55374342-55374361	MS.gene041359:intron	25.0%
!	ATCTATGATGAAAACAATGA+TGG	-	chr2.1:55374150-55374169	MS.gene041359:intron	25.0%
!!	ATCATTCATTTTCTTCTTCT+TGG	+	chr2.1:55374337-55374356	None:intergenic	25.0%
!!!	GTTTTTTTTTATCACTACCA+TGG	-	chr2.1:55373795-55373814	MS.gene041359:CDS	25.0%
	AGAGAAGTTGAAGAAACAAA+AGG	-	chr2.1:55374305-55374324	MS.gene041359:intron	30.0%
	CTTCTCTTTCGATTTCATTA+TGG	+	chr2.1:55374292-55374311	None:intergenic	30.0%
	GAAGATCTATCATTGCTTAT+TGG	-	chr2.1:55374417-55374436	MS.gene041359:CDS	30.0%
!	ACTTGGTGAATTCATTCATA+CGG	-	chr2.1:55374359-55374378	MS.gene041359:intron	30.0%
!!	GTTTTGTTGTGTTTTTCAAC+AGG	-	chr2.1:55374069-55374088	MS.gene041359:intron	30.0%
	AAGTTGACTGCATAACCATT+AGG	-	chr2.1:55373833-55373852	MS.gene041359:CDS	35.0%
	ACATAACTGATGACTTGAAG+AGG	-	chr2.1:55373655-55373674	MS.gene041359:CDS	35.0%
	ACATTCAACAAAAGGAAGCA+TGG	-	chr2.1:55373684-55373703	MS.gene041359:CDS	35.0%
	ACGGTTTATCCATCAAAAGA+AGG	-	chr2.1:55374177-55374196	MS.gene041359:intron	35.0%
	AGTTGAAGAAACAAAAGGAC+AGG	-	chr2.1:55374310-55374329	MS.gene041359:intron	35.0%
	ATTCATACGGGTGAATTTGA+TGG	-	chr2.1:55374372-55374391	MS.gene041359:intron	35.0%
	CTTGGTGAATTCATTCATAC+GGG	-	chr2.1:55374360-55374379	MS.gene041359:intron	35.0%
	GGAAGACAACATTCAACAAA+AGG	-	chr2.1:55373676-55373695	MS.gene041359:CDS	35.0%
!	TGAAAACAATGATGGAGCAA+CGG	-	chr2.1:55374158-55374177	MS.gene041359:intron	35.0%
	AAAAGGAAAGCTGGATCATG+AGG	-	chr2.1:55374251-55374270	MS.gene041359:intron	40.0%
	AAAGGAAAGCTGGATCATGA+GGG	-	chr2.1:55374252-55374271	MS.gene041359:intron	40.0%
	AGTGAAATCACCACACTTTG+TGG	-	chr2.1:55374108-55374127	MS.gene041359:intron	40.0%
	ATCAAAAGAAGGAGTCCAGA+TGG	-	chr2.1:55374188-55374207	MS.gene041359:intron	40.0%
	ATTTCTCTCAACTCTCTCAC+GGG	-	chr2.1:55374047-55374066	MS.gene041359:intron	40.0%
	CATTTCTCTCAACTCTCTCA+CGG	-	chr2.1:55374046-55374065	MS.gene041359:intron	40.0%
	CCTGAATCGGAACAAAGAAA+AGG	-	chr2.1:55374234-55374253	MS.gene041359:intron	40.0%
	CTTTGTGGAATTGATGCATG+TGG	-	chr2.1:55374123-55374142	MS.gene041359:intron	40.0%
	GGAACAAAGAAAAGGAAAGC+TGG	-	chr2.1:55374242-55374261	MS.gene041359:intron	40.0%
	GTTTAGGAGCTTTCCTGAAT+CGG	-	chr2.1:55374221-55374240	MS.gene041359:intron	40.0%
	TAAACTTGTCCAACACCATC+TGG	+	chr2.1:55374206-55374225	None:intergenic	40.0%
	TGCATCAATTCCACAAAGTG+TGG	+	chr2.1:55374121-55374140	None:intergenic	40.0%
!	AGATGGTGTTGGACAAGTTT+AGG	-	chr2.1:55374205-55374224	MS.gene041359:intron	40.0%
!	ATCTGGACTCCTTCTTTTGA+TGG	+	chr2.1:55374189-55374208	None:intergenic	40.0%
!	CCTTTTCTTTGTTCCGATTC+AGG	+	chr2.1:55374237-55374256	None:intergenic	40.0%
!!!	TCCTCTCATCGACTTCTTTT+CGG	+	chr2.1:55374449-55374468	None:intergenic	40.0%
	ACCGAAAAGAAGTCGATGAG+AGG	-	chr2.1:55374445-55374464	MS.gene041359:CDS	45.0%
	AGAAGGAGTCCAGATGGTGT+TGG	-	chr2.1:55374194-55374213	MS.gene041359:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	55373627	55374488	55373627	ID=MS.gene041359
chr2.1	mRNA	55373627	55374488	55373627	ID=MS.gene041359.t1;Parent=MS.gene041359
chr2.1	exon	55374410	55374488	55374410	ID=MS.gene041359.t1.exon1;Parent=MS.gene041359.t1
chr2.1	CDS	55374410	55374488	55374410	ID=cds.MS.gene041359.t1;Parent=MS.gene041359.t1
chr2.1	exon	55373627	55374024	55373627	ID=MS.gene041359.t1.exon2;Parent=MS.gene041359.t1
chr2.1	CDS	55373627	55374024	55373627	ID=cds.MS.gene041359.t1;Parent=MS.gene041359.t1

Gene Sequence

>MS.gene041359

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTTAGATACTAAATTTCTCCTTTATTTCTATAAAGAACACTCATATCATACAAATTTATTATGAATTTTTTTCTTGTTCATTTCTTTCAGTTTTTTTTTATCACTACCATGGATACTAAAAATATGAAAGTTGACTGCATAACCATTAGGAATTTATCAATATGAATATAGTTATTTGATGTTTGTTATTTTTTTTGTTGTTGTTGATATCATGAAAAAATTGTAAGCAAATGTTGTTGCAACCATAATAACATATCAGTTATGTAGACCTTTGAAATTATTGTTATTTTCACAATATTTTAAGATCATGTCGGTTATAGTATACATTTTTTCAGTTTCACATTTCTCTCAACTCTCTCACGGGTTTTGTTGTGTTTTTCAACAGGTATAACTAAGAAGATTAGTGAAATCACCACACTTTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACGGGTGAATTTGATGGAAATGTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>MS.gene041359.t1

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNVSENDLEDLSLLIGDYRKEVDERIESSQT