Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041513.t1 | XP_003609586.1 | 91.2 | 170 | 15 | 0 | 1 | 170 | 12 | 181 | 8.50E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041513.t1 | G7JJ33 | 91.2 | 170 | 15 | 0 | 1 | 170 | 12 | 181 | 6.1e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene041513.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041513.t1 | MTR_4g118830 | 91.176 | 170 | 15 | 0 | 1 | 170 | 12 | 181 | 2.34e-108 | 307 |
| MS.gene041513.t1 | MTR_4g118430 | 45.946 | 111 | 41 | 3 | 66 | 170 | 1 | 98 | 3.31e-21 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGCTCTGTTACGGTTAC+TGG | 0.180827 | 8.2:-11329370 | MS.gene041513:CDS |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8.2:-11329346 | MS.gene041513:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8.2:-11329399 | MS.gene041513:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8.2:-11329161 | None:intergenic |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8.2:-11329345 | MS.gene041513:CDS |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8.2:-11329205 | MS.gene041513:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8.2:-11329215 | MS.gene041513:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8.2:+11329680 | None:intergenic |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8.2:-11329230 | MS.gene041513:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8.2:-11329461 | MS.gene041513:CDS |
| GAATCTCAATTGCTCTGTTA+CGG | 0.448731 | 8.2:-11329377 | MS.gene041513:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8.2:+11329679 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8.2:-11329431 | MS.gene041513:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8.2:-11329473 | MS.gene041513:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8.2:-11329244 | MS.gene041513:CDS |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8.2:-11329332 | MS.gene041513:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8.2:+11329695 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8.2:-11329322 | MS.gene041513:CDS |
| TTCAACGTTTAGAACCTCTT+CGG | 0.524218 | 8.2:+11329756 | None:intergenic |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8.2:-11329433 | MS.gene041513:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8.2:-11329470 | MS.gene041513:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8.2:-11329432 | MS.gene041513:CDS |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8.2:-11329744 | MS.gene041513:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8.2:-11329430 | MS.gene041513:CDS |
| CACTGCTACTAAAACCGAAG+AGG | 0.637311 | 8.2:-11329770 | MS.gene041513:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8.2:+11329304 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8.2:-11329356 | MS.gene041513:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8.2:+11329687 | None:intergenic |
| TACACAGGAAGTGATTGTGA+AGG | 0.673700 | 8.2:-11329521 | MS.gene041513:CDS |
| TTGAGATTCCTATTACGACA+CGG | 0.693765 | 8.2:+11329391 | None:intergenic |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8.2:-11329793 | MS.gene041513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | + | chr8.2:11329313-11329332 | None:intergenic | 15.0% |
| !! | ATAACAAATCAAACTATAAT+GGG | + | chr8.2:11329386-11329405 | None:intergenic | 15.0% |
| !!! | TTTATTTTCATTCTTCATTT+GGG | - | chr8.2:11329321-11329340 | MS.gene041513:CDS | 15.0% |
| !!! | TTTTATTTTCATTCTTCATT+TGG | - | chr8.2:11329320-11329339 | MS.gene041513:CDS | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | + | chr8.2:11329309-11329328 | None:intergenic | 20.0% |
| !! | GATAACAAATCAAACTATAA+TGG | + | chr8.2:11329387-11329406 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | - | chr8.2:11329393-11329412 | MS.gene041513:CDS | 20.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | - | chr8.2:11329722-11329741 | MS.gene041513:CDS | 25.0% |
| ! | TGAAGAAAAGAAGAAAATGT+TGG | - | chr8.2:11329479-11329498 | MS.gene041513:CDS | 25.0% |
| !! | TTTGATTACTGTGATTTTGA+AGG | - | chr8.2:11329653-11329672 | MS.gene041513:intron | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | - | chr8.2:11329392-11329411 | MS.gene041513:CDS | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | - | chr8.2:11329407-11329426 | MS.gene041513:CDS | 25.0% |
| AAAATCACAGTAATCAAACC+AGG | + | chr8.2:11329651-11329670 | None:intergenic | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | - | chr8.2:11329519-11329538 | MS.gene041513:CDS | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | - | chr8.2:11329520-11329539 | MS.gene041513:CDS | 30.0% | |
| AGAAAAGAAGAAAATGTTGG+AGG | - | chr8.2:11329482-11329501 | MS.gene041513:CDS | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | + | chr8.2:11329289-11329308 | None:intergenic | 30.0% | |
| GAATGATGTTAGTTACATTG+GGG | + | chr8.2:11329287-11329306 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | + | chr8.2:11329288-11329307 | None:intergenic | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | - | chr8.2:11329708-11329727 | MS.gene041513:CDS | 30.0% |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | - | chr8.2:11329416-11329435 | MS.gene041513:CDS | 30.0% |
| AAATTAAAACCCCCTTCAAC+AGG | + | chr8.2:11329534-11329553 | None:intergenic | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | - | chr8.2:11329521-11329540 | MS.gene041513:CDS | 35.0% | |
| GAATCTCAATTGCTCTGTTA+CGG | - | chr8.2:11329575-11329594 | MS.gene041513:intron | 35.0% | |
| TAAACGTTGAACGTGAAAGA+AGG | - | chr8.2:11329208-11329227 | MS.gene041513:CDS | 35.0% | |
| TTGAGATTCCTATTACGACA+CGG | + | chr8.2:11329564-11329583 | None:intergenic | 35.0% | |
| ! | ATCACTGCTTTGGTTCAAAA+TGG | - | chr8.2:11329747-11329766 | MS.gene041513:CDS | 35.0% |
| ! | TTCAACGTTTAGAACCTCTT+CGG | + | chr8.2:11329199-11329218 | None:intergenic | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | - | chr8.2:11329677-11329696 | MS.gene041513:intron | 35.0% |
| !! | TTTCTTTGGGATTGAATCTG+TGG | - | chr8.2:11329620-11329639 | MS.gene041513:intron | 35.0% |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | - | chr8.2:11329536-11329555 | MS.gene041513:intron | 35.0% |
| AATTGCTCTGTTACGGTTAC+TGG | - | chr8.2:11329582-11329601 | MS.gene041513:intron | 40.0% | |
| ATTGAATCTGTGGCTAAACC+TGG | - | chr8.2:11329630-11329649 | MS.gene041513:intron | 40.0% | |
| CAAGCAATGTGGCTTTCTTT+GGG | - | chr8.2:11329607-11329626 | MS.gene041513:intron | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | - | chr8.2:11329491-11329510 | MS.gene041513:CDS | 40.0% | |
| TACACAGGAAGTGATTGTGA+AGG | - | chr8.2:11329431-11329450 | MS.gene041513:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | - | chr8.2:11329522-11329541 | MS.gene041513:CDS | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | - | chr8.2:11329606-11329625 | MS.gene041513:intron | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | + | chr8.2:11329275-11329294 | None:intergenic | 40.0% | |
| CACTGCTACTAAAACCGAAG+AGG | - | chr8.2:11329182-11329201 | MS.gene041513:CDS | 45.0% | |
| GTTACATTGGGGTAGAGAGT+TGG | + | chr8.2:11329276-11329295 | None:intergenic | 45.0% | |
| !! | GGTTACTGGTTCAAGCAATG+TGG | - | chr8.2:11329596-11329615 | MS.gene041513:intron | 45.0% |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | - | chr8.2:11329553-11329572 | MS.gene041513:intron | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | - | chr8.2:11329737-11329756 | MS.gene041513:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | + | chr8.2:11329260-11329279 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | + | chr8.2:11329268-11329287 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 11329162 | 11329812 | 11329162 | ID=MS.gene041513 |
| chr8.2 | mRNA | 11329162 | 11329812 | 11329162 | ID=MS.gene041513.t1;Parent=MS.gene041513 |
| chr8.2 | exon | 11329684 | 11329812 | 11329684 | ID=MS.gene041513.t1.exon1;Parent=MS.gene041513.t1 |
| chr8.2 | CDS | 11329684 | 11329812 | 11329684 | ID=cds.MS.gene041513.t1;Parent=MS.gene041513.t1 |
| chr8.2 | exon | 11329162 | 11329545 | 11329162 | ID=MS.gene041513.t1.exon2;Parent=MS.gene041513.t1 |
| chr8.2 | CDS | 11329162 | 11329545 | 11329162 | ID=cds.MS.gene041513.t1;Parent=MS.gene041513.t1 |
| Gene Sequence |
| Protein sequence |
