AlfalfaGEDB | MS.gene041548

M. sativa cultivar XinJiangDaYe / MS.gene041548

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041548.t1	AFK36846.1	99.2	129	1	0	1	129	1	129	2.60E-66	261.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041548.t1	O82248	78.9	95	20	0	22	116	51	145	5.2e-39	161.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041548.t1	G7JKC3	99.2	129	1	0	1	129	1	129	1.9e-66	261.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene041548.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041548.t1	MTR_4g119500	99.225	129	1	0	1	129	1	129	9.30e-95	268
MS.gene041548.t1	MTR_0392s0020	86.614	127	12	4	2	128	1	122	4.61e-72	212
MS.gene041548.t1	MTR_1g088860	74.107	112	29	0	5	116	19	130	2.75e-61	186
MS.gene041548.t1	MTR_4g133938	67.273	110	31	1	12	116	4	113	5.53e-51	158
MS.gene041548.t1	MTR_2g026710	61.157	121	44	1	12	129	4	124	1.77e-50	158
MS.gene041548.t1	MTR_1g072790	66.990	103	34	0	21	123	28	130	3.84e-50	157
MS.gene041548.t1	MTR_7g061270	62.162	111	40	1	7	115	9	119	7.17e-49	154
MS.gene041548.t1	MTR_7g100650	62.162	111	40	1	7	115	9	119	9.75e-49	154
MS.gene041548.t1	MTR_3g058980	59.322	118	45	1	3	117	5	122	3.75e-48	153
MS.gene041548.t1	MTR_8g091720	62.617	107	40	0	21	127	27	133	4.76e-48	153
MS.gene041548.t1	MTR_5g095740	67.010	97	32	0	21	117	22	118	7.84e-48	152
MS.gene041548.t1	MTR_1g083070	72.632	95	26	0	22	116	4	98	1.28e-47	149
MS.gene041548.t1	MTR_8g093920	69.892	93	28	0	21	113	39	131	2.57e-47	152
MS.gene041548.t1	MTR_4g133952	60.000	110	44	0	19	128	55	164	2.74e-47	152
MS.gene041548.t1	MTR_1g039040	63.830	94	34	0	20	113	4	97	2.94e-42	138
MS.gene041548.t1	MTR_1g029070	47.872	94	49	0	21	114	51	144	1.64e-26	97.4
MS.gene041548.t1	MTR_5g095900	46.237	93	50	0	19	111	13	105	4.88e-25	94.0
MS.gene041548.t1	MTR_1g028480	48.529	68	35	0	47	114	14	81	1.18e-18	75.9
MS.gene041548.t1	MTR_1g029100	36.250	80	51	0	27	106	7	86	4.45e-17	71.2
MS.gene041548.t1	MTR_2g056000	37.179	78	49	0	23	100	42	119	4.58e-16	70.9
MS.gene041548.t1	MTR_4g112380	30.682	88	61	0	23	110	12	99	4.24e-15	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041548.t1	AT2G47810	77.551	98	22	0	19	116	48	145	7.59e-56	171
MS.gene041548.t1	AT2G38880	69.307	101	31	0	15	115	14	114	1.90e-51	159
MS.gene041548.t1	AT2G38880	69.307	101	31	0	15	115	14	114	2.29e-51	159
MS.gene041548.t1	AT2G38880	69.307	101	31	0	15	115	14	114	2.29e-51	159
MS.gene041548.t1	AT2G38880	69.307	101	31	0	15	115	14	114	2.29e-51	159
MS.gene041548.t1	AT4G14540	60.504	119	44	1	3	121	5	120	1.02e-50	159
MS.gene041548.t1	AT1G09030	71.429	98	28	0	20	117	1	98	6.33e-50	155
MS.gene041548.t1	AT5G47640	66.667	108	32	1	21	124	26	133	7.32e-50	157
MS.gene041548.t1	AT2G38880	68.367	98	31	0	15	112	14	111	1.10e-49	154
MS.gene041548.t1	AT2G38880	69.072	97	30	0	15	111	14	110	1.75e-49	154
MS.gene041548.t1	AT2G38880	68.367	98	31	0	15	112	14	111	3.40e-49	155
MS.gene041548.t1	AT2G38880	68.367	98	31	0	15	112	14	111	3.40e-49	155
MS.gene041548.t1	AT2G38880	68.367	98	31	0	15	112	14	111	4.62e-49	154
MS.gene041548.t1	AT2G38880	68.367	98	31	0	15	112	14	111	4.62e-49	154
MS.gene041548.t1	AT2G38880	68.367	98	31	0	15	112	14	111	4.62e-49	154
MS.gene041548.t1	AT3G53340	66.337	101	34	0	20	120	27	127	4.69e-49	154
MS.gene041548.t1	AT3G53340	66.337	101	34	0	20	120	27	127	4.69e-49	154
MS.gene041548.t1	AT3G53340	68.750	96	30	0	20	115	27	122	1.30e-48	154
MS.gene041548.t1	AT3G53340	68.750	96	30	0	20	115	27	122	1.30e-48	154
MS.gene041548.t1	AT2G37060	67.708	96	31	0	20	115	28	123	8.09e-48	151
MS.gene041548.t1	AT2G37060	67.708	96	31	0	20	115	28	123	8.09e-48	151
MS.gene041548.t1	AT2G37060	67.708	96	31	0	20	115	28	123	8.09e-48	151
MS.gene041548.t1	AT3G53340	68.817	93	29	0	20	112	27	119	1.95e-47	149
MS.gene041548.t1	AT2G13570	60.345	116	46	0	1	116	15	130	2.13e-47	152
MS.gene041548.t1	AT5G47670	61.905	105	36	1	16	116	19	123	4.75e-45	145
MS.gene041548.t1	AT5G47670	61.905	105	36	1	16	116	48	152	7.43e-45	146
MS.gene041548.t1	AT5G47670	61.905	105	36	1	16	116	48	152	7.43e-45	146
MS.gene041548.t1	AT1G21970	52.632	114	45	1	12	116	40	153	1.07e-40	135
MS.gene041548.t1	AT5G08190	30.612	98	66	1	22	117	11	108	1.11e-15	69.3
MS.gene041548.t1	AT5G08190	30.851	94	63	1	22	113	11	104	3.24e-15	68.2
MS.gene041548.t1	AT5G23090	30.928	97	65	1	23	117	12	108	4.25e-15	67.8
MS.gene041548.t1	AT5G23090	31.183	93	62	1	23	113	12	104	2.59e-14	65.9
MS.gene041548.t1	AT5G23090	31.183	93	62	1	23	113	12	104	2.59e-14	65.9
MS.gene041548.t1	AT5G23090	31.183	93	62	1	23	113	12	104	2.59e-14	65.9

Find 25 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGATATCTGTTGGGCTTT+GGG	0.254012	8.2:+10928893	MS.gene041548:CDS
TGATGATATCTGTTGGGCTT+TGG	0.268785	8.2:+10928892	MS.gene041548:CDS
GCATAGATATAGAGAATTAG+AGG	0.332962	8.2:+10928961	MS.gene041548:CDS
ATCCTCCCAACATTTGCTAT+TGG	0.373158	8.2:-10928725	None:intergenic
TGAATATTATTAGCAGTAGT+TGG	0.384741	8.2:-10928995	None:intergenic
TGTTGGGCTTTGGGGACACT+TGG	0.405725	8.2:+10928902	MS.gene041548:CDS
CTATGCAAATACCTTCTCAT+TGG	0.433135	8.2:-10928944	None:intergenic
GCTAATAATATTCAAGATAG+AGG	0.434183	8.2:+10929004	MS.gene041548:CDS
TGAAAACGGAGGCATAATAA+AGG	0.463124	8.2:+10928685	MS.gene041548:CDS
GCATAATAAAGGAACAAGAT+AGG	0.472463	8.2:+10928696	MS.gene041548:CDS
TTGCTACCAATAGCAAATGT+TGG	0.481402	8.2:+10928719	MS.gene041548:CDS
TGCTACCAATAGCAAATGTT+GGG	0.486912	8.2:+10928720	MS.gene041548:CDS
GTGAAGCATCAGAGAAATGC+AGG	0.487879	8.2:+10928843	MS.gene041548:CDS
GAAAGGAGAAAGACAGTGAA+TGG	0.488041	8.2:+10928869	MS.gene041548:CDS
ATGATATCTGTTGGGCTTTG+GGG	0.489965	8.2:+10928894	MS.gene041548:CDS
TCCTTTAGTAATGATGAAAA+CGG	0.512602	8.2:+10928671	MS.gene041548:CDS
CAGAGAAATGCAGGAAGGAA+AGG	0.518431	8.2:+10928852	MS.gene041548:CDS
GAATGGTGATGATATCTGTT+GGG	0.569420	8.2:+10928886	MS.gene041548:CDS
ATGATGGATAACAACGTTGG+AGG	0.607529	8.2:+10928644	MS.gene041548:CDS
TGAATGGTGATGATATCTGT+TGG	0.622578	8.2:+10928885	MS.gene041548:CDS
GAAATGATGGATAACAACGT+TGG	0.629287	8.2:+10928641	None:intergenic
TTTAGTAATGATGAAAACGG+AGG	0.646824	8.2:+10928674	MS.gene041548:CDS
ATTACGCAGAACCAATGAGA+AGG	0.655098	8.2:+10928933	MS.gene041548:CDS
TACCAATAGCAAATGTTGGG+AGG	0.664077	8.2:+10928723	MS.gene041548:CDS
AGCATCAGAGAAATGCAGGA+AGG	0.676487	8.2:+10928847	MS.gene041548:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAATCTCAAAGGAATCAA+AGG	+	chr8.2:10928775-10928794	MS.gene041548:CDS	25.0%
!	GCTAATAATATTCAAGATAG+AGG	+	chr8.2:10929004-10929023	MS.gene041548:CDS	25.0%
!	TCCTTTAGTAATGATGAAAA+CGG	+	chr8.2:10928671-10928690	MS.gene041548:CDS	25.0%
!	TGAATATTATTAGCAGTAGT+TGG	-	chr8.2:10928998-10929017	None:intergenic	25.0%
	ACAGAATGCAAAAATCTCAA+AGG	+	chr8.2:10928766-10928785	MS.gene041548:CDS	30.0%
	GCATAATAAAGGAACAAGAT+AGG	+	chr8.2:10928696-10928715	MS.gene041548:CDS	30.0%
	GCATAGATATAGAGAATTAG+AGG	+	chr8.2:10928961-10928980	MS.gene041548:CDS	30.0%
	TTTAGTAATGATGAAAACGG+AGG	+	chr8.2:10928674-10928693	MS.gene041548:CDS	30.0%
!	TCCGTTTTCATCATTACTAA+AGG	-	chr8.2:10928675-10928694	None:intergenic	30.0%
!!	TTTGAGATTTTTGCATTCTG+TGG	-	chr8.2:10928767-10928786	None:intergenic	30.0%
	CTATGCAAATACCTTCTCAT+TGG	-	chr8.2:10928947-10928966	None:intergenic	35.0%
	TGCTACCAATAGCAAATGTT+GGG	+	chr8.2:10928720-10928739	MS.gene041548:CDS	35.0%
	TTGCTACCAATAGCAAATGT+TGG	+	chr8.2:10928719-10928738	MS.gene041548:CDS	35.0%
!	GAATGGTGATGATATCTGTT+GGG	+	chr8.2:10928886-10928905	MS.gene041548:CDS	35.0%
!	TGAAAACGGAGGCATAATAA+AGG	+	chr8.2:10928685-10928704	MS.gene041548:CDS	35.0%
!	TGAATGGTGATGATATCTGT+TGG	+	chr8.2:10928885-10928904	MS.gene041548:CDS	35.0%
	ATGATATCTGTTGGGCTTTG+GGG	+	chr8.2:10928894-10928913	MS.gene041548:CDS	40.0%
	ATTACGCAGAACCAATGAGA+AGG	+	chr8.2:10928933-10928952	MS.gene041548:CDS	40.0%
	GAAAGGAGAAAGACAGTGAA+TGG	+	chr8.2:10928869-10928888	MS.gene041548:CDS	40.0%
	GATGATATCTGTTGGGCTTT+GGG	+	chr8.2:10928893-10928912	MS.gene041548:CDS	40.0%
	TACCAATAGCAAATGTTGGG+AGG	+	chr8.2:10928723-10928742	MS.gene041548:CDS	40.0%
	TGATGATATCTGTTGGGCTT+TGG	+	chr8.2:10928892-10928911	MS.gene041548:CDS	40.0%
!!	ATCCTCCCAACATTTGCTAT+TGG	-	chr8.2:10928728-10928747	None:intergenic	40.0%
!!	ATGATGGATAACAACGTTGG+AGG	+	chr8.2:10928644-10928663	MS.gene041548:CDS	40.0%
	AGCATCAGAGAAATGCAGGA+AGG	+	chr8.2:10928847-10928866	MS.gene041548:CDS	45.0%
	CAGAGAAATGCAGGAAGGAA+AGG	+	chr8.2:10928852-10928871	MS.gene041548:CDS	45.0%
!	GTGAAGCATCAGAGAAATGC+AGG	+	chr8.2:10928843-10928862	MS.gene041548:CDS	45.0%
!!	TGTTGGGCTTTGGGGACACT+TGG	+	chr8.2:10928902-10928921	MS.gene041548:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	10928644	10929033	10928644	ID=MS.gene041548
chr8.2	mRNA	10928644	10929033	10928644	ID=MS.gene041548.t1;Parent=MS.gene041548
chr8.2	exon	10928644	10929033	10928644	ID=MS.gene041548.t1.exon1;Parent=MS.gene041548.t1
chr8.2	CDS	10928644	10929033	10928644	ID=cds.MS.gene041548.t1;Parent=MS.gene041548.t1

Gene Sequence

>MS.gene041548

ATGATGGATAACAACGTTGGAGGAAGTTCCTTTAGTAATGATGAAAACGGAGGCATAATAAAGGAACAAGATAGGTTGCTACCAATAGCAAATGTTGGGAGGATAATGAAGCAGATATTACCACAGAATGCAAAAATCTCAAAGGAATCAAAGGAAACAATGCAAGAGTGTGTTTCAGAGTTTATAAGCTTTGTGACAAGTGAAGCATCAGAGAAATGCAGGAAGGAAAGGAGAAAGACAGTGAATGGTGATGATATCTGTTGGGCTTTGGGGACACTTGGTTTTGATGATTACGCAGAACCAATGAGAAGGTATTTGCATAGATATAGAGAATTAGAGGTAGATAGAACACCAACTACTGCTAATAATATTCAAGATAGAGGAAATTAA

Protein sequence

>MS.gene041548.t1

MMDNNVGGSSFSNDENGGIIKEQDRLLPIANVGRIMKQILPQNAKISKESKETMQECVSEFISFVTSEASEKCRKERRKTVNGDDICWALGTLGFDDYAEPMRRYLHRYRELEVDRTPTTANNIQDRGN