AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041645

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041645.t1 MTR_8g017210 65.385 156 53 1 29 184 62 216 2.26e-74 243
MS.gene041645.t1 MTR_1g016010 61.404 171 59 3 21 185 28 197 7.41e-73 237
MS.gene041645.t1 MTR_2g099990 63.462 156 56 1 29 184 46 200 1.61e-72 236
MS.gene041645.t1 MTR_1g094115 62.179 156 58 1 29 184 47 201 1.43e-71 236
MS.gene041645.t1 MTR_2g093960 62.179 156 57 2 29 183 32 186 3.80e-70 229
MS.gene041645.t1 MTR_1g112270 61.438 153 58 1 32 184 47 198 7.96e-70 232
MS.gene041645.t1 MTR_4g059870 61.783 157 58 2 29 184 38 193 2.05e-69 229
MS.gene041645.t1 MTR_1g112270 62.069 145 54 1 40 184 66 209 1.11e-66 224
MS.gene041645.t1 MTR_2g090745 63.514 148 53 1 37 184 15 161 8.66e-66 218
MS.gene041645.t1 MTR_2g099990 63.636 132 47 1 53 184 1 131 3.30e-60 202
MS.gene041645.t1 MTR_1g094115 54.487 156 53 2 29 184 47 184 4.89e-57 197
MS.gene041645.t1 MTR_2g017720 60.870 115 39 1 39 153 323 431 2.86e-40 150
MS.gene041645.t1 MTR_3g102000 47.015 134 60 4 25 151 2 131 6.88e-28 111
MS.gene041645.t1 MTR_5g030560 64.444 45 16 0 39 83 16 60 2.87e-16 73.9
MS.gene041645.t1 MTR_4g103250 39.241 79 48 0 360 438 62 140 4.56e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041645.t1 AT1G68130 48.238 454 140 10 22 457 34 410 1.20e-127 377
MS.gene041645.t1 AT2G01940 81.643 207 29 4 21 218 32 238 2.04e-122 365
MS.gene041645.t1 AT2G01940 79.904 209 30 6 21 218 32 239 4.65e-117 351
MS.gene041645.t1 AT1G25250 81.026 195 32 2 22 211 26 220 8.70e-114 340
MS.gene041645.t1 AT1G25250 43.704 135 69 2 329 457 249 382 1.09e-22 100
MS.gene041645.t1 AT1G25250 81.461 178 28 2 39 211 20 197 9.65e-103 311
MS.gene041645.t1 AT1G25250 43.704 135 69 2 329 457 226 359 7.39e-23 100
MS.gene041645.t1 AT1G68130 42.893 401 134 10 75 457 1 324 1.21e-88 274
MS.gene041645.t1 AT2G01940 80.000 150 25 3 75 219 1 150 1.86e-83 261
MS.gene041645.t1 AT1G25250 77.465 142 27 2 75 211 1 142 7.95e-74 235
MS.gene041645.t1 AT1G25250 43.704 135 69 2 329 457 171 304 2.99e-23 100
MS.gene041645.t1 AT5G66730 64.151 159 56 1 26 184 29 186 1.40e-72 238
MS.gene041645.t1 AT3G50700 59.195 174 65 2 26 194 31 203 3.22e-72 235
MS.gene041645.t1 AT1G55110 63.125 160 57 2 26 184 60 218 6.18e-71 232
MS.gene041645.t1 AT1G55110 63.125 160 57 2 26 184 60 218 6.18e-71 232
MS.gene041645.t1 AT1G55110 63.125 160 57 2 26 184 60 218 6.18e-71 232
MS.gene041645.t1 AT2G02080 58.896 163 66 1 22 184 47 208 9.38e-71 233
MS.gene041645.t1 AT2G02080 58.896 163 66 1 22 184 47 208 9.38e-71 233
MS.gene041645.t1 AT2G02080 58.896 163 66 1 22 184 47 208 9.38e-71 233
MS.gene041645.t1 AT2G02080 58.896 163 66 1 22 184 47 208 9.38e-71 233
MS.gene041645.t1 AT5G03150 62.112 161 59 2 25 184 49 208 3.21e-70 232
MS.gene041645.t1 AT1G03840 63.462 156 56 1 29 184 41 195 3.60e-70 231
MS.gene041645.t1 AT4G02670 58.757 177 66 3 29 199 55 230 1.83e-69 227
MS.gene041645.t1 AT4G02670 58.757 177 66 3 29 199 53 228 1.93e-69 227
MS.gene041645.t1 AT3G13810 62.500 160 58 2 25 183 47 205 7.13e-69 228
MS.gene041645.t1 AT1G14580 58.333 156 64 1 29 184 53 207 7.55e-69 227
MS.gene041645.t1 AT1G14580 58.333 156 64 1 29 184 53 207 7.55e-69 227
MS.gene041645.t1 AT3G13810 62.500 160 58 2 25 183 66 224 8.15e-69 228
MS.gene041645.t1 AT3G13810 62.500 160 58 2 25 183 66 224 8.15e-69 228
MS.gene041645.t1 AT3G13810 62.500 160 58 2 25 183 61 219 8.67e-69 228
MS.gene041645.t1 AT1G14580 58.333 156 64 1 29 184 67 221 1.36e-68 227
MS.gene041645.t1 AT5G44160 62.179 156 58 1 29 184 37 191 3.49e-68 225
MS.gene041645.t1 AT5G60470 50.490 204 94 3 6 208 22 219 4.40e-68 225
MS.gene041645.t1 AT5G60470 50.490 204 94 3 6 208 26 223 4.97e-68 224
MS.gene041645.t1 AT2G02070 59.615 156 62 1 29 184 52 206 2.71e-67 226
MS.gene041645.t1 AT2G02070 59.615 156 62 1 29 184 52 206 2.71e-67 226
MS.gene041645.t1 AT3G45260 59.873 157 61 2 29 184 39 194 3.23e-67 222
MS.gene041645.t1 AT3G45260 59.873 157 61 2 29 184 39 194 3.23e-67 222
MS.gene041645.t1 AT1G03840 62.179 156 56 2 29 184 41 193 1.65e-66 222
MS.gene041645.t1 AT5G44160 63.014 146 53 1 39 184 8 152 1.13e-64 215
MS.gene041645.t1 AT3G13810 64.138 145 50 2 40 183 68 211 9.82e-64 214
MS.gene041645.t1 AT3G13810 64.138 145 50 2 40 183 82 225 1.36e-63 214
MS.gene041645.t1 AT5G60470 53.529 170 77 2 40 208 46 214 1.86e-61 207
MS.gene041645.t1 AT2G02080 59.091 132 53 1 53 184 1 131 5.00e-58 198
MS.gene041645.t1 AT2G02080 59.091 132 53 1 53 184 1 131 5.00e-58 198
MS.gene041645.t1 AT5G22890 31.737 167 94 7 28 179 190 351 2.19e-17 84.0
MS.gene041645.t1 AT1G34370 31.210 157 91 5 38 179 14 168 1.16e-16 80.9
MS.gene041645.t1 AT1G34370 31.210 157 91 5 38 179 224 378 1.95e-16 82.0
MS.gene041645.t1 AT1G34370 31.210 157 91 5 38 179 224 378 1.95e-16 82.0
MS.gene041645.t1 AT3G48550 41.772 79 46 0 360 438 79 157 8.77e-16 75.1
MS.gene041645.t1 AT3G20880 29.252 147 94 4 50 188 248 392 6.85e-12 67.8

Find 82 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTATGCATCTGTAAATTT+TGG 0.191998 6.3:-69399004 None:intergenic
ATAGTAGTAATAGGAGTAAT+AGG 0.205036 6.3:-69400814 None:intergenic
GCTCTTCTTCCACTAATAAA+AGG 0.263568 6.3:+69398517 MS.gene041645:CDS
TTGAAATTTGTTTCACTTGA+TGG 0.265033 6.3:-69400721 None:intergenic
AGTGAACTTGATAAAGCTTA+TGG 0.272526 6.3:+69401300 MS.gene041645:CDS
CATGATCCTTGTCATGCTTT+AGG 0.279693 6.3:+69399119 MS.gene041645:CDS
GAAGGTGTAATAGTAGTAAT+AGG 0.296600 6.3:-69400823 None:intergenic
ACAAGCAACGGAACAATTAA+AGG 0.306652 6.3:+69401164 MS.gene041645:CDS
GAGTAGAAGTTTATTGTTCT+TGG 0.315722 6.3:-69400860 None:intergenic
TCAACATGAAGATAACTCTT+TGG 0.326169 6.3:+69401398 MS.gene041645:CDS
ACTCAGTTGCATCTCTCAAT+AGG 0.329653 6.3:+69401003 MS.gene041645:CDS
GAGAGTTGAAGTTCCAAGTT+TGG 0.344241 6.3:-69400886 None:intergenic
TTGCTATTGCTACTCTCCTT+TGG 0.344778 6.3:-69401087 None:intergenic
TTTGGTCCCTTTGGAATCCT+TGG 0.356941 6.3:-69398986 None:intergenic
TTGGAGAAAGTTTGAGAAGA+AGG 0.371907 6.3:-69400841 None:intergenic
TTCACCACCTTCAGTAGTTA+TGG 0.411636 6.3:-69401442 None:intergenic
GTGGTTGAGATTCTGAATTA+TGG 0.414564 6.3:-69400651 None:intergenic
AGAGACACCACTTCTGCATC+TGG 0.419684 6.3:-69398912 None:intergenic
TCTCTCTTCAATAGCTTCCA+TGG 0.428102 6.3:-69399041 None:intergenic
AGATTGCACTGCATAACCTT+TGG 0.429152 6.3:-69399216 None:intergenic
AGCAAGGAAACAAATTGAAA+TGG 0.432424 6.3:+69401230 MS.gene041645:CDS
TGGTGCAAACAAGTTGGTTC+AGG 0.440705 6.3:-69399098 None:intergenic
AGAAGACAAACCTGGTGTTC+CGG 0.442410 6.3:-69398553 None:intergenic
GTAGAAAATGTTGATGGAAA+AGG 0.444254 6.3:+69401465 MS.gene041645:CDS
CTCAGTTGCATCTCTCAATA+GGG 0.455582 6.3:+69401004 MS.gene041645:CDS
CGTGTTGGTGTTGTTTGTGG+TGG 0.457194 6.3:-69400908 None:intergenic
CATGCTTTAGGTGATCTTGT+TGG 0.466169 6.3:+69399131 MS.gene041645:CDS
TTTGTTTCACTTGATGGACT+TGG 0.468237 6.3:-69400715 None:intergenic
ACTTCTACTCCATCCAAACT+TGG 0.469640 6.3:+69400873 MS.gene041645:CDS
GTACCAACACTTCTGATTGA+AGG 0.475892 6.3:-69400949 None:intergenic
TCAGAAGATGGAAGTGAAGA+TGG 0.478640 6.3:-69398471 None:intergenic
AAACAACATGGCTTTGTTGA+GGG 0.481856 6.3:+69401134 MS.gene041645:CDS
AGTCGTGTTGGTGTTGTTTG+TGG 0.482657 6.3:-69400911 None:intergenic
TAGTAGTAATAGGAGTAATA+GGG 0.486179 6.3:-69400813 None:intergenic
AGGCTATTCAGAAGGTTAAC+CGG 0.501979 6.3:+69400977 MS.gene041645:CDS
ACATAGTAATCATAAACAAT+GGG 0.505280 6.3:+69399178 MS.gene041645:CDS
AGAAGACCGGCCGGAACACC+AGG 0.510779 6.3:+69398543 MS.gene041645:CDS
CATCAAGATGCTTGCAACAT+GGG 0.511025 6.3:+69400622 MS.gene041645:CDS
GTTGAAGTTCCAAGTTTGGA+TGG 0.511669 6.3:-69400882 None:intergenic
GGTGAAGTAGAAAATGTTGA+TGG 0.512722 6.3:+69401459 MS.gene041645:CDS
GTATGTGAGATCTGCAACCA+AGG 0.515942 6.3:+69398969 MS.gene041645:CDS
CAAACAACATGGCTTTGTTG+AGG 0.518950 6.3:+69401133 MS.gene041645:CDS
GGAACAATTAAAGGTAGCTA+TGG 0.527664 6.3:+69401173 MS.gene041645:CDS
AACATAGTAATCATAAACAA+TGG 0.531879 6.3:+69399177 MS.gene041645:CDS
CTAAAGCATGACAAGGATCA+TGG 0.532248 6.3:-69399118 None:intergenic
ATTTACAGATGCATAGAAGA+AGG 0.537823 6.3:+69399009 MS.gene041645:CDS
GTCTGTTGTCAGAGTCGTGT+TGG 0.543599 6.3:-69400923 None:intergenic
GATGCATAGAAGAAGGCATA+AGG 0.546337 6.3:+69399016 MS.gene041645:CDS
TCTTCTGCCATAACTACTGA+AGG 0.549896 6.3:+69401435 MS.gene041645:CDS
GCTTGAAGCCTTGTTTGCCA+TGG 0.551999 6.3:-69400751 None:intergenic
GCATCAAGATGCTTGCAACA+TGG 0.560086 6.3:+69400621 MS.gene041645:CDS
GACAAACCTGGTGTTCCGGC+CGG 0.564975 6.3:-69398549 None:intergenic
AGAGATGCAACTGAGTTGAC+CGG 0.567581 6.3:-69400996 None:intergenic
AAGATGCTTGCAACATGGGA+AGG 0.571020 6.3:+69400626 MS.gene041645:CDS
CTCAAAACTTGTGGTACTAG+AGG 0.574559 6.3:+69399254 MS.gene041645:CDS
ATACATGTCCATGGCAAACA+AGG 0.574678 6.3:+69400743 MS.gene041645:CDS
AGATAAGCTATTAGCTAACA+TGG 0.580180 6.3:-69398448 None:intergenic
TACTGTGGAGGCTATTCAGA+AGG 0.581212 6.3:+69400969 MS.gene041645:CDS
CATGCAGTTGGTGTTTGAAG+TGG 0.583027 6.3:-69400670 None:intergenic
CAAATTTCAATACATGTCCA+TGG 0.586561 6.3:+69400734 MS.gene041645:CDS
AAAGCTCATCTCAAAACTTG+TGG 0.590538 6.3:+69399245 MS.gene041645:CDS
AGAGGTCATTCATGTGATTG+TGG 0.593681 6.3:+69399272 MS.gene041645:CDS
AAGCTTATGCTGAAGAAGCA+AGG 0.595645 6.3:+69401202 MS.gene041645:CDS
AATCAGAAGTGTTGGTACTG+TGG 0.596959 6.3:+69400954 MS.gene041645:CDS
TAATAAAAGGAAAAGAAGAC+CGG 0.599740 6.3:+69398530 MS.gene041645:CDS
GTTTGTGAAAGATGTTCCAA+AGG 0.605307 6.3:+69399200 MS.gene041645:CDS
GTTCTTGACAAGCATGCAGT+TGG 0.605757 6.3:-69400682 None:intergenic
CTGCAACCAAGGATTCCAAA+GGG 0.607708 6.3:+69398980 MS.gene041645:CDS
GGATCATGGTGCAAACAAGT+TGG 0.608116 6.3:-69399104 None:intergenic
AAAAGGAAAAGAAGACCGGC+CGG 0.608705 6.3:+69398534 MS.gene041645:CDS
ACGCCTTCAATCAGAAGTGT+TGG 0.611782 6.3:+69400946 MS.gene041645:CDS
GAAGAAGGCATAAGGTACCA+TGG 0.614434 6.3:+69399024 MS.gene041645:CDS
AAGAAGCAAGGAAACAAGCA+AGG 0.615543 6.3:+69401214 MS.gene041645:CDS
AATAGATCCAGATGCAGAAG+TGG 0.615906 6.3:+69398905 MS.gene041645:intron
ATTGTGGTCGTGTCTTCTCA+AGG 0.631038 6.3:+69399288 MS.gene041645:CDS
CAGAAGTGTTGGTACTGTGG+AGG 0.634164 6.3:+69400957 MS.gene041645:CDS
AAACTCAAACTCATCACCAA+AGG 0.637316 6.3:+69401071 MS.gene041645:CDS
TCTGCAACCAAGGATTCCAA+AGG 0.641443 6.3:+69398979 MS.gene041645:CDS
GAGGGTTCAAGAACAAGCAA+CGG 0.651961 6.3:+69401152 MS.gene041645:CDS
TCTGCCATAACTACTGAAGG+TGG 0.661155 6.3:+69401438 MS.gene041645:CDS
ATCAACAACAACAAACAACA+TGG 0.678900 6.3:+69401122 MS.gene041645:CDS
AGATCACCTAAAGCATGACA+AGG 0.685132 6.3:-69399125 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATAATATTAA+TGG - chr6.3:69399973-69399992 None:intergenic 0.0%
!!! TTATTTATTTTTTTTAAATA+TGG + chr6.3:69398766-69398785 MS.gene041645:intron 0.0%
!! AATATAAAAGTAAAGAAAAT+GGG + chr6.3:69399663-69399682 MS.gene041645:intron 10.0%
!! CTACAAAAAATATATATAAA+TGG - chr6.3:69400583-69400602 None:intergenic 10.0%
!! TATAAAAAAATACTTGAAAA+AGG - chr6.3:69399378-69399397 None:intergenic 10.0%
!!! AATTTTTATATATACTTCAT+GGG + chr6.3:69399495-69399514 MS.gene041645:intron 10.0%
!!! CATTTATATATATTTTTTGT+AGG + chr6.3:69400581-69400600 MS.gene041645:intron 10.0%
!!! TTTGAAACAAAAATATAAAA+AGG - chr6.3:69400251-69400270 None:intergenic 10.0%
!! AAAACATAAAGAAATGTTTA+AGG - chr6.3:69399924-69399943 None:intergenic 15.0%
!! GAATATAAAAGTAAAGAAAA+TGG + chr6.3:69399662-69399681 MS.gene041645:intron 15.0%
!! GTCAATTATTATTGTTAATT+AGG + chr6.3:69400394-69400413 MS.gene041645:intron 15.0%
!! TAAATTTACATATACTAGTA+GGG + chr6.3:69400532-69400551 MS.gene041645:intron 15.0%
!! TATTACTATTTAAAGAATTG+AGG - chr6.3:69400484-69400503 None:intergenic 15.0%
!!! AAATTGTTCTTTATTTTGTT+TGG + chr6.3:69398877-69398896 MS.gene041645:intron 15.0%
!!! GAATTTTTATATATACTTCA+TGG + chr6.3:69399494-69399513 MS.gene041645:intron 15.0%
!! AAAAAACATGATTCAAGAAT+TGG - chr6.3:69398666-69398685 None:intergenic 20.0%
!! AACATAGTAATCATAAACAA+TGG + chr6.3:69399177-69399196 MS.gene041645:CDS 20.0%
!! ACATAGTAATCATAAACAAT+GGG + chr6.3:69399178-69399197 MS.gene041645:CDS 20.0%
!! ATGATGATTATAATGAATTG+AGG + chr6.3:69399577-69399596 MS.gene041645:intron 20.0%
!! GTAAATTTACATATACTAGT+AGG + chr6.3:69400531-69400550 MS.gene041645:intron 20.0%
!! TAATAATAAAATCACACACA+TGG - chr6.3:69400342-69400361 None:intergenic 20.0%
!! TCAAAAATATATTTGACTGA+TGG + chr6.3:69399727-69399746 MS.gene041645:intron 20.0%
!! TTCATTAGATATAAAGAGTT+AGG - chr6.3:69400226-69400245 None:intergenic 20.0%
!! TTGATTCTTGATTCATAAAA+TGG + chr6.3:69399427-69399446 MS.gene041645:intron 20.0%
!!! AAAAAGGAAGATATGTTTTT+TGG + chr6.3:69399635-69399654 MS.gene041645:intron 20.0%
!!! TCATGTTTTTTTTGTTGTAT+TGG + chr6.3:69398675-69398694 MS.gene041645:intron 20.0%
!!! TTTTGTCCTTTTTATTTTAG+TGG + chr6.3:69400069-69400088 MS.gene041645:intron 20.0%
!!! TTTTTGAAGAAAACACAAAA+CGG - chr6.3:69399862-69399881 None:intergenic 20.0%
! AAAAATGTACAAAGGAAGAA+AGG - chr6.3:69400202-69400221 None:intergenic 25.0%
! AAAAGTAAAGAAAATGGGAA+TGG + chr6.3:69399668-69399687 MS.gene041645:intron 25.0%
! AATGAAAAAGTGGATGTAAA+AGG - chr6.3:69400161-69400180 None:intergenic 25.0%
! AGTTAGGAAAAAATGTACAA+AGG - chr6.3:69400210-69400229 None:intergenic 25.0%
! ATAGTAGTAATAGGAGTAAT+AGG - chr6.3:69400817-69400836 None:intergenic 25.0%
! ATGAATCAAGAATCAATCAT+AGG - chr6.3:69399423-69399442 None:intergenic 25.0%
! CAGTTTCCACTAAAATAAAA+AGG - chr6.3:69400078-69400097 None:intergenic 25.0%
! TAATAAAAGGAAAAGAAGAC+CGG + chr6.3:69398530-69398549 MS.gene041645:CDS 25.0%
! TAGTAGTAATAGGAGTAATA+GGG - chr6.3:69400816-69400835 None:intergenic 25.0%
! TTATATATACTTCATGGGAT+GGG + chr6.3:69399500-69399519 MS.gene041645:intron 25.0%
! TTTATATATACTTCATGGGA+TGG + chr6.3:69399499-69399518 MS.gene041645:intron 25.0%
! TTTGTGTAGAAAGAATGAAA+TGG - chr6.3:69399893-69399912 None:intergenic 25.0%
!! AAAACTCTTTTTCTAACAAC+TGG - chr6.3:69399071-69399090 None:intergenic 25.0%
!! TCTTCTTTTCCTTTTATTAG+TGG - chr6.3:69398529-69398548 None:intergenic 25.0%
!! TTCTATGCATCTGTAAATTT+TGG - chr6.3:69399007-69399026 None:intergenic 25.0%
!! TTGAAATTTGTTTCACTTGA+TGG - chr6.3:69400724-69400743 None:intergenic 25.0%
!! TTGGGATTTGACTTTAAAAA+AGG + chr6.3:69399619-69399638 MS.gene041645:intron 25.0%
!!! AAAACTTGAGTTGTTTTACT+TGG + chr6.3:69399600-69399619 MS.gene041645:intron 25.0%
!!! AAACTTGAGTTGTTTTACTT+GGG + chr6.3:69399601-69399620 MS.gene041645:intron 25.0%
!!! CTTTTTATTTTAGTGGAAAC+TGG + chr6.3:69400076-69400095 MS.gene041645:intron 25.0%
!!! GTTCTTTATTTTGTTTGGTT+TGG + chr6.3:69398882-69398901 MS.gene041645:intron 25.0%
!!! GTTGATTCTTTTGTTGTTTT+TGG - chr6.3:69400781-69400800 None:intergenic 25.0%
AAGAGAGACAAATGAAAAAG+TGG - chr6.3:69400171-69400190 None:intergenic 30.0%
AATGGGAATGGAAAAACAAA+AGG + chr6.3:69399680-69399699 MS.gene041645:intron 30.0%
AGCAAGGAAACAAATTGAAA+TGG + chr6.3:69401230-69401249 MS.gene041645:CDS 30.0%
AGTGAACTTGATAAAGCTTA+TGG + chr6.3:69401300-69401319 MS.gene041645:CDS 30.0%
ATCAACAACAACAAACAACA+TGG + chr6.3:69401122-69401141 MS.gene041645:CDS 30.0%
ATTTACAGATGCATAGAAGA+AGG + chr6.3:69399009-69399028 MS.gene041645:CDS 30.0%
CAAATTTCAATACATGTCCA+TGG + chr6.3:69400734-69400753 MS.gene041645:CDS 30.0%
GAGTAGAAGTTTATTGTTCT+TGG - chr6.3:69400863-69400882 None:intergenic 30.0%
TCAACATGAAGATAACTCTT+TGG + chr6.3:69401398-69401417 MS.gene041645:CDS 30.0%
! AAAAAGAAAGAGGATTTTGG+AGG - chr6.3:69398582-69398601 None:intergenic 30.0%
! CAATTCAGCTTTATGTTTCT+GGG + chr6.3:69400455-69400474 MS.gene041645:intron 30.0%
! CCATTTTCATTGCAAGAAAA+AGG - chr6.3:69398498-69398517 None:intergenic 30.0%
! CCTTTTTCTTGCAATGAAAA+TGG + chr6.3:69398495-69398514 MS.gene041645:CDS 30.0%
! GAAGGTGTAATAGTAGTAAT+AGG - chr6.3:69400826-69400845 None:intergenic 30.0%
! GTAGAAAATGTTGATGGAAA+AGG + chr6.3:69401465-69401484 MS.gene041645:CDS 30.0%
! TCTGATTCCAACAATGTTTT+TGG - chr6.3:69398942-69398961 None:intergenic 30.0%
!!! AGGAAGATATGTTTTTTGGA+AGG + chr6.3:69399639-69399658 MS.gene041645:intron 30.0%
!!! GGAAGATATGTTTTTTGGAA+GGG + chr6.3:69399640-69399659 MS.gene041645:intron 30.0%
AAACTCAAACTCATCACCAA+AGG + chr6.3:69401071-69401090 MS.gene041645:CDS 35.0%
AAAGCTCATCTCAAAACTTG+TGG + chr6.3:69399245-69399264 MS.gene041645:CDS 35.0%
ACAAGCAACGGAACAATTAA+AGG + chr6.3:69401164-69401183 MS.gene041645:CDS 35.0%
CAAAACGGTTATAAGAGCAA+CGG - chr6.3:69399847-69399866 None:intergenic 35.0%
GCTCTTCTTCCACTAATAAA+AGG + chr6.3:69398517-69398536 MS.gene041645:CDS 35.0%
GGAACAATTAAAGGTAGCTA+TGG + chr6.3:69401173-69401192 MS.gene041645:CDS 35.0%
GGTGAAGTAGAAAATGTTGA+TGG + chr6.3:69401459-69401478 MS.gene041645:CDS 35.0%
GTGGTTGAGATTCTGAATTA+TGG - chr6.3:69400654-69400673 None:intergenic 35.0%
GTTATAAGAGCAACGGAAAA+CGG - chr6.3:69399840-69399859 None:intergenic 35.0%
GTTTGTGAAAGATGTTCCAA+AGG + chr6.3:69399200-69399219 MS.gene041645:CDS 35.0%
TCTCTCACCAAAAACATTGT+TGG + chr6.3:69398932-69398951 MS.gene041645:CDS 35.0%
TTGGAGAAAGTTTGAGAAGA+AGG - chr6.3:69400844-69400863 None:intergenic 35.0%
! AAAGTGGATGTAAAAGGTGT+TGG - chr6.3:69400155-69400174 None:intergenic 35.0%
! GCAATTCAGCTTTATGTTTC+TGG + chr6.3:69400454-69400473 MS.gene041645:intron 35.0%
! GCGAAAAAGAAAGAGGATTT+TGG - chr6.3:69398585-69398604 None:intergenic 35.0%
! GTTAGAGTTTAGAAGTACGA+AGG - chr6.3:69398622-69398641 None:intergenic 35.0%
! TATCCACTGGATTTTCCAAA+TGG - chr6.3:69400122-69400141 None:intergenic 35.0%
!! AAACAACATGGCTTTGTTGA+GGG + chr6.3:69401134-69401153 MS.gene041645:CDS 35.0%
!! GATGTAAAAGGTGTTGGAAA+AGG - chr6.3:69400149-69400168 None:intergenic 35.0%
!! TCTGTAAATTTTGGTCCCTT+TGG - chr6.3:69398998-69399017 None:intergenic 35.0%
!! TTTGTTTCACTTGATGGACT+TGG - chr6.3:69400718-69400737 None:intergenic 35.0%
AAGAAGCAAGGAAACAAGCA+AGG + chr6.3:69401214-69401233 MS.gene041645:CDS 40.0%
AAGCTTATGCTGAAGAAGCA+AGG + chr6.3:69401202-69401221 MS.gene041645:CDS 40.0%
AATAGATCCAGATGCAGAAG+TGG + chr6.3:69398905-69398924 MS.gene041645:intron 40.0%
AATCACACACATGGATGAAG+AGG - chr6.3:69400333-69400352 None:intergenic 40.0%
ACTCAGTTGCATCTCTCAAT+AGG + chr6.3:69401003-69401022 MS.gene041645:CDS 40.0%
ACTTCTACTCCATCCAAACT+TGG + chr6.3:69400873-69400892 MS.gene041645:CDS 40.0%
AGAGGTCATTCATGTGATTG+TGG + chr6.3:69399272-69399291 MS.gene041645:CDS 40.0%
AGATCACCTAAAGCATGACA+AGG - chr6.3:69399128-69399147 None:intergenic 40.0%
AGATTGCACTGCATAACCTT+TGG - chr6.3:69399219-69399238 None:intergenic 40.0%
AGGCTATTCAGAAGGTTAAC+CGG + chr6.3:69400977-69400996 MS.gene041645:CDS 40.0%
ATACATGTCCATGGCAAACA+AGG + chr6.3:69400743-69400762 MS.gene041645:CDS 40.0%
CAAGAAAAAGGCTCAGAAGA+TGG - chr6.3:69398486-69398505 None:intergenic 40.0%
CATCAAGATGCTTGCAACAT+GGG + chr6.3:69400622-69400641 MS.gene041645:CDS 40.0%
CATGATCCTTGTCATGCTTT+AGG + chr6.3:69399119-69399138 MS.gene041645:CDS 40.0%
CTAAAGCATGACAAGGATCA+TGG - chr6.3:69399121-69399140 None:intergenic 40.0%
CTCAAAACTTGTGGTACTAG+AGG + chr6.3:69399254-69399273 MS.gene041645:CDS 40.0%
CTCAGTTGCATCTCTCAATA+GGG + chr6.3:69401004-69401023 MS.gene041645:CDS 40.0%
GAGAGTTGAAGTTCCAAGTT+TGG - chr6.3:69400889-69400908 None:intergenic 40.0%
GTACCAACACTTCTGATTGA+AGG - chr6.3:69400952-69400971 None:intergenic 40.0%
GTTGAAGTTCCAAGTTTGGA+TGG - chr6.3:69400885-69400904 None:intergenic 40.0%
TCACACTGTTCATTGACAGT+TGG + chr6.3:69399794-69399813 MS.gene041645:intron 40.0%
TCAGAAGATGGAAGTGAAGA+TGG - chr6.3:69398474-69398493 None:intergenic 40.0%
TCTCTCTTCAATAGCTTCCA+TGG - chr6.3:69399044-69399063 None:intergenic 40.0%
TCTTCTGCCATAACTACTGA+AGG + chr6.3:69401435-69401454 MS.gene041645:CDS 40.0%
TTCACCACCTTCAGTAGTTA+TGG - chr6.3:69401445-69401464 None:intergenic 40.0%
! ACTGGATTTTCCAAATGGTG+GGG - chr6.3:69400117-69400136 None:intergenic 40.0%
! CACTGGATTTTCCAAATGGT+GGG - chr6.3:69400118-69400137 None:intergenic 40.0%
! CATGCTTTAGGTGATCTTGT+TGG + chr6.3:69399131-69399150 MS.gene041645:CDS 40.0%
! GATGCATAGAAGAAGGCATA+AGG + chr6.3:69399016-69399035 MS.gene041645:CDS 40.0%
! TTGCTATTGCTACTCTCCTT+TGG - chr6.3:69401090-69401109 None:intergenic 40.0%
!! AATCAGAAGTGTTGGTACTG+TGG + chr6.3:69400954-69400973 MS.gene041645:CDS 40.0%
!! ATCCTCTTTCTTTTTCGCAC+TGG + chr6.3:69398587-69398606 MS.gene041645:intron 40.0%
!! CAAACAACATGGCTTTGTTG+AGG + chr6.3:69401133-69401152 MS.gene041645:CDS 40.0%
!! TCCTCTTTCTTTTTCGCACT+GGG + chr6.3:69398588-69398607 MS.gene041645:intron 40.0%
!! TGGAAAAGGTATCTATCCAC+TGG - chr6.3:69400135-69400154 None:intergenic 40.0%
AAAAGGAAAAGAAGACCGGC+CGG + chr6.3:69398534-69398553 MS.gene041645:CDS 45.0%
AAGATGCTTGCAACATGGGA+AGG + chr6.3:69400626-69400645 MS.gene041645:CDS 45.0%
ACCCAGTGCGAAAAAGAAAG+AGG - chr6.3:69398592-69398611 None:intergenic 45.0%
AGAAGACAAACCTGGTGTTC+CGG - chr6.3:69398556-69398575 None:intergenic 45.0%
ATTGTGGTCGTGTCTTCTCA+AGG + chr6.3:69399288-69399307 MS.gene041645:CDS 45.0%
CCACCATTTGGAAAATCCAG+TGG + chr6.3:69400116-69400135 MS.gene041645:intron 45.0%
CTGCAACCAAGGATTCCAAA+GGG + chr6.3:69398980-69398999 MS.gene041645:CDS 45.0%
GAGGGTTCAAGAACAAGCAA+CGG + chr6.3:69401152-69401171 MS.gene041645:CDS 45.0%
GCATCAAGATGCTTGCAACA+TGG + chr6.3:69400621-69400640 MS.gene041645:CDS 45.0%
GGATCATGGTGCAAACAAGT+TGG - chr6.3:69399107-69399126 None:intergenic 45.0%
GTATGTGAGATCTGCAACCA+AGG + chr6.3:69398969-69398988 MS.gene041645:CDS 45.0%
GTTCTTGACAAGCATGCAGT+TGG - chr6.3:69400685-69400704 None:intergenic 45.0%
TACTGTGGAGGCTATTCAGA+AGG + chr6.3:69400969-69400988 MS.gene041645:CDS 45.0%
TCTGCAACCAAGGATTCCAA+AGG + chr6.3:69398979-69398998 MS.gene041645:CDS 45.0%
TCTGCCATAACTACTGAAGG+TGG + chr6.3:69401438-69401457 MS.gene041645:CDS 45.0%
! ACGCCTTCAATCAGAAGTGT+TGG + chr6.3:69400946-69400965 MS.gene041645:CDS 45.0%
! AGAGATGCAACTGAGTTGAC+CGG - chr6.3:69400999-69401018 None:intergenic 45.0%
! CATGCAGTTGGTGTTTGAAG+TGG - chr6.3:69400673-69400692 None:intergenic 45.0%
! CCACTGGATTTTCCAAATGG+TGG - chr6.3:69400119-69400138 None:intergenic 45.0%
! GAAGAAGGCATAAGGTACCA+TGG + chr6.3:69399024-69399043 MS.gene041645:CDS 45.0%
!! AGTCGTGTTGGTGTTGTTTG+TGG - chr6.3:69400914-69400933 None:intergenic 45.0%
!! GATTTTCCAAATGGTGGGGT+GGG - chr6.3:69400113-69400132 None:intergenic 45.0%
!! TGGTGCAAACAAGTTGGTTC+AGG - chr6.3:69399101-69399120 None:intergenic 45.0%
!! TTTGGTCCCTTTGGAATCCT+TGG - chr6.3:69398989-69399008 None:intergenic 45.0%
!!! ATACATTTTTTTTTATATTA+AGG - chr6.3:69399755-69399774 None:intergenic 5.0%
!!! ATTTATATATATTTTTTGTA+GGG + chr6.3:69400582-69400601 MS.gene041645:intron 5.0%
AGAGACACCACTTCTGCATC+TGG - chr6.3:69398915-69398934 None:intergenic 50.0%
AGCAACGGAAAACGGAGCAT+TGG - chr6.3:69399832-69399851 None:intergenic 50.0%
GGAGGAGAAGAAGACAAACC+TGG - chr6.3:69398564-69398583 None:intergenic 50.0%
GTCTGTTGTCAGAGTCGTGT+TGG - chr6.3:69400926-69400945 None:intergenic 50.0%
!! CAGAAGTGTTGGTACTGTGG+AGG + chr6.3:69400957-69400976 MS.gene041645:CDS 50.0%
!! CGTGTTGGTGTTGTTTGTGG+TGG - chr6.3:69400911-69400930 None:intergenic 50.0%
!! GCTTGAAGCCTTGTTTGCCA+TGG - chr6.3:69400754-69400773 None:intergenic 50.0%
!! GGATTTTCCAAATGGTGGGG+TGG - chr6.3:69400114-69400133 None:intergenic 50.0%
CTTCTTCCCACCCCACCATT+TGG + chr6.3:69400104-69400123 MS.gene041645:intron 55.0%
! AATGGTGGGGTGGGAAGAAG+AGG - chr6.3:69400104-69400123 None:intergenic 55.0%
GACAAACCTGGTGTTCCGGC+CGG - chr6.3:69398552-69398571 None:intergenic 60.0%
AGAAGACCGGCCGGAACACC+AGG + chr6.3:69398543-69398562 MS.gene041645:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 69398450 69401503 69398450 ID=MS.gene041645
chr6.3 mRNA 69398450 69401503 69398450 ID=MS.gene041645.t1;Parent=MS.gene041645
chr6.3 exon 69398450 69398564 69398450 ID=MS.gene041645.t1.exon1;Parent=MS.gene041645.t1
chr6.3 CDS 69398450 69398564 69398450 ID=cds.MS.gene041645.t1;Parent=MS.gene041645.t1
chr6.3 exon 69398910 69399309 69398910 ID=MS.gene041645.t1.exon2;Parent=MS.gene041645.t1
chr6.3 CDS 69398910 69399309 69398910 ID=cds.MS.gene041645.t1;Parent=MS.gene041645.t1
chr6.3 exon 69400603 69401503 69400603 ID=MS.gene041645.t1.exon3;Parent=MS.gene041645.t1
chr6.3 CDS 69400603 69401503 69400603 ID=cds.MS.gene041645.t1;Parent=MS.gene041645.t1
Gene Sequence

>MS.gene041645

ATGTTAGCTAATAGCTTATCTCCATCTTCACTTCCATCTTCTGAGCCTTTTTCTTGCAATGAAAATGGCTCTTCTTCCACTAATAAAAGGAAAAGAAGACCGGCCGGAACACCAGGTTTGTCTTCTTCTCCTCCAAAATCCTCTTTCTTTTTCGCACTGGGTTGAGTATCCTTCGTACTTCTAAACTCTAACTAGTGTTAGAGAAAATCTCAACCAATTCTTGAATCATGTTTTTTTTGTTGTATTGGATTCGACTATCGTTCTCAAGTCTATAAAAAAAACATTCTTTTTGACATGTTTTTTTCATTCTCTTGATTTATTTATTTTTTTTAAATATGGTTGTTACTTATATGTTTGTATGTTAATATTGTTTATTTAATTCAATATGTATACTTTTTTATAATTGTTTGATTTGATACTAATAAATAAATTGTTCTTTATTTTGTTTGGTTTGGAATAGATCCAGATGCAGAAGTGGTGTCTCTCTCACCAAAAACATTGTTGGAATCAGATCGTTATGTATGTGAGATCTGCAACCAAGGATTCCAAAGGGACCAAAATTTACAGATGCATAGAAGAAGGCATAAGGTACCATGGAAGCTATTGAAGAGAGAAACACCAGTTGTTAGAAAAAGAGTTTTTGTTTGTCCTGAACCAACTTGTTTGCACCATGATCCTTGTCATGCTTTAGGTGATCTTGTTGGTATCAAAAAACATTTTAGAAGAAAACATAGTAATCATAAACAATGGGTTTGTGAAAGATGTTCCAAAGGTTATGCAGTGCAATCTGATTATAAAGCTCATCTCAAAACTTGTGGTACTAGAGGTCATTCATGTGATTGTGGTCGTGTCTTCTCAAGGTAATTAATTAAATTATTTATTTTTCTTTCATTCATAATTTCATATATCTAAATATATAATTATACCTTTTTCAAGTATTTTTTTATAAATATATATTATCAACATTTATCCTATGATTGATTCTTGATTCATAAAATGGTTTTGAGCAAATAGATCTAGCTATATATAGAATTTTTATTTTGAGAATTTTTATATATACTTCATGGGATGGGATACTATAGTTTTTTTTTTTTCTTTTTAAATGAAACATTTTTAAACATTGTCTTATGATGATTATAATGAATTGAGGAAAACTTGAGTTGTTTTACTTGGGATTTGACTTTAAAAAAGGAAGATATGTTTTTTGGAAGGGAATATAAAAGTAAAGAAAATGGGAATGGAAAAACAAAAGGAGAGATAATTAAAATAAAAGCTTTTCAAAAATATATTTGACTGATGGAACCTTAATATAAAAAAAAATGTATTTTATTTTTCTTAACAAGATCACACTGTTCATTGACAGTTGGAGAACACAGTTTCCAATGCTCCGTTTTCCGTTGCTCTTATAACCGTTTTGTGTTTTCTTCAAAAATCATTCTTCCATTTCATTCTTTCTACACAAACAACAATACCTTAAACATTTCTTTATGTTTTTTTCATGTATATGTTTGTGTGAGAAACCATTAATATTATTTTTTTTTTTATAACATTGCAATTTAATTTCTTCTTAACAGTGAATCACATGAAAATGTGATTACTCATGAGAGTTCTTGACTATGTTTTGTCCTTTTTATTTTAGTGGAAACTGGAACCTCTTCTTCCCACCCCACCATTTGGAAAATCCAGTGGATAGATACCTTTTCCAACACCTTTTACATCCACTTTTTCATTTGTCTCTCTTTCTTTCTACCTTTCTTCCTTTGTACATTTTTTCCTAACTCTTTATATCTAATGAATTCCTTTTTATATTTTTGTTTCAAATCTCAATACTAGTAGCTTGTACTAAATTACAAATACTAACAAATGTTTCTTTTTCTCATCCTCTTCATCCATGTGTGTGATTTTATTATTATATATATAAAACACTATTATTAAATATTTATAGTCAATTATTATTGTTAATTAGGATTATGATGATGATGATAATAACACACATAGTATTTTGCAATTCAGCTTTATGTTTCTGGGTTTCCTCAATTCTTTAAATAGTAATATTTTGTTGTTCAAAGATATTTGTATGAGTAAATTTACATATACTAGTAGGGTTTTTTTTTCTCATCTTGTTTTCTTCCATTTATATATATTTTTTGTAGGGTTGAGAGCTTTATTGAGCATCAAGATGCTTGCAACATGGGAAGGATCCATAATTCAGAATCTCAACCACTTCAAACACCAACTGCATGCTTGTCAAGAACAGCTTCAAGTCCAAGTCCATCAAGTGAAACAAATTTCAATACATGTCCATGGCAAACAAGGCTTCAAGCAATGCCAAAAACAACAAAAGAATCAACAATATTCATGAACCCTATTACTCCTATTACTACTATTACACCTTCTTCTCAAACTTTCTCCAAGAACAATAAACTTCTACTCCATCCAAACTTGGAACTTCAACTCTCCACCACAAACAACACCAACACGACTCTGACAACAGACACGCCTTCAATCAGAAGTGTTGGTACTGTGGAGGCTATTCAGAAGGTTAACCGGTCAACTCAGTTGCATCTCTCAATAGGGTCTTCAGAAATGAGTCATGAGAAAAATGAATCAAACAATATTAGAAACTCAAACTCATCACCAAAGGAGAGTAGCAATAGCAATGAGAAAGTTCAATCAACAACAACAAACAACATGGCTTTGTTGAGGGTTCAAGAACAAGCAACGGAACAATTAAAGGTAGCTATGGCTGAAAAAGCTTATGCTGAAGAAGCAAGGAAACAAGCAAGGAAACAAATTGAAATGGCTGAACAAGAGTTCAACAATGCAAAGAGAATTAGACAACAAGCACAAAGTGAACTTGATAAAGCTTATGGATTGAAACAACATGCAATAAAGCAAATCAATTCAACTATGCTTCAAATCACTTGTCAAGCTTGCAAACAACAATTTCAACATGAAGATAACTCTTTGGTGTTGAGCTATGTTTCTTCTGCCATAACTACTGAAGGTGGTGAAGTAGAAAATGTTGATGGAAAAGGCAAAACAACTAATTAG
Protein sequence

>MS.gene041645.t1

MLANSLSPSSLPSSEPFSCNENGSSSTNKRKRRPAGTPDPDAEVVSLSPKTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRETPVVRKRVFVCPEPTCLHHDPCHALGDLVGIKKHFRRKHSNHKQWVCERCSKGYAVQSDYKAHLKTCGTRGHSCDCGRVFSRVESFIEHQDACNMGRIHNSESQPLQTPTACLSRTASSPSPSSETNFNTCPWQTRLQAMPKTTKESTIFMNPITPITTITPSSQTFSKNNKLLLHPNLELQLSTTNNTNTTLTTDTPSIRSVGTVEAIQKVNRSTQLHLSIGSSEMSHEKNESNNIRNSNSSPKESSNSNEKVQSTTTNNMALLRVQEQATEQLKVAMAEKAYAEEARKQARKQIEMAEQEFNNAKRIRQQAQSELDKAYGLKQHAIKQINSTMLQITCQACKQQFQHEDNSLVLSYVSSAITTEGGEVENVDGKGKTTN