Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041664.t1 | XP_024632961.1 | 90.9 | 230 | 21 | 0 | 1 | 230 | 81 | 310 | 4.30E-120 | 440.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041664.t1 | Q9SWG3 | 24.7 | 227 | 154 | 5 | 5 | 230 | 81 | 291 | 1.7e-11 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041664.t1 | A0A072TE13 | 89.6 | 230 | 24 | 0 | 1 | 230 | 66 | 295 | 3.8e-118 | 433.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene041664.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041664.t1 | MTR_0558s0020 | 89.565 | 230 | 24 | 0 | 1 | 230 | 66 | 295 | 9.86e-161 | 446 |
| MS.gene041664.t1 | MTR_6g090505 | 85.652 | 230 | 33 | 0 | 1 | 230 | 1 | 230 | 3.49e-150 | 422 |
| MS.gene041664.t1 | MTR_1g083640 | 90.686 | 204 | 19 | 0 | 1 | 204 | 81 | 284 | 1.28e-143 | 402 |
| MS.gene041664.t1 | MTR_8g446020 | 80.176 | 227 | 45 | 0 | 4 | 230 | 29 | 255 | 1.01e-138 | 389 |
| MS.gene041664.t1 | MTR_2g014100 | 87.209 | 172 | 22 | 0 | 1 | 172 | 74 | 245 | 6.48e-113 | 323 |
| MS.gene041664.t1 | MTR_0337s0040 | 77.720 | 193 | 43 | 0 | 4 | 196 | 30 | 222 | 9.01e-113 | 322 |
| MS.gene041664.t1 | MTR_1g055240 | 88.028 | 142 | 17 | 0 | 27 | 168 | 3 | 144 | 6.03e-93 | 270 |
| MS.gene041664.t1 | MTR_0019s0290 | 82.237 | 152 | 27 | 0 | 18 | 169 | 9 | 160 | 5.98e-92 | 267 |
| MS.gene041664.t1 | MTR_3g008240 | 86.957 | 138 | 18 | 0 | 1 | 138 | 66 | 203 | 5.19e-87 | 256 |
| MS.gene041664.t1 | MTR_4g040430 | 86.232 | 138 | 15 | 1 | 1 | 138 | 65 | 198 | 6.49e-85 | 251 |
| MS.gene041664.t1 | MTR_5g071590 | 83.168 | 101 | 17 | 0 | 1 | 101 | 78 | 178 | 9.90e-62 | 191 |
| MS.gene041664.t1 | MTR_1281s0010 | 82.540 | 63 | 11 | 0 | 1 | 63 | 47 | 109 | 5.22e-32 | 113 |
| MS.gene041664.t1 | MTR_6g051425 | 33.824 | 204 | 123 | 3 | 21 | 224 | 64 | 255 | 2.77e-30 | 115 |
| MS.gene041664.t1 | MTR_1g070355 | 27.053 | 207 | 137 | 2 | 21 | 227 | 119 | 311 | 8.05e-18 | 82.0 |
| MS.gene041664.t1 | MTR_0337s0050 | 75.000 | 36 | 9 | 0 | 195 | 230 | 14 | 49 | 2.05e-14 | 66.6 |
| MS.gene041664.t1 | MTR_0402s0040 | 26.000 | 150 | 96 | 3 | 20 | 169 | 71 | 205 | 5.53e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041664.t1 | AT4G38180 | 31.308 | 214 | 123 | 5 | 20 | 228 | 118 | 312 | 2.66e-26 | 107 |
| MS.gene041664.t1 | AT5G28530 | 23.377 | 231 | 152 | 5 | 5 | 223 | 84 | 301 | 1.34e-12 | 67.0 |
| MS.gene041664.t1 | AT5G28530 | 23.377 | 231 | 152 | 5 | 5 | 223 | 84 | 301 | 1.34e-12 | 67.0 |
| MS.gene041664.t1 | AT5G28530 | 23.377 | 231 | 152 | 5 | 5 | 223 | 84 | 301 | 1.42e-12 | 67.0 |
| MS.gene041664.t1 | AT4G15090 | 27.746 | 173 | 122 | 3 | 59 | 230 | 121 | 291 | 6.39e-12 | 65.1 |
| MS.gene041664.t1 | AT4G15090 | 27.746 | 173 | 122 | 3 | 59 | 230 | 121 | 291 | 6.39e-12 | 65.1 |
| MS.gene041664.t1 | AT4G15090 | 27.746 | 173 | 122 | 3 | 59 | 230 | 121 | 291 | 6.39e-12 | 65.1 |
| MS.gene041664.t1 | AT4G15090 | 27.746 | 173 | 122 | 3 | 59 | 230 | 121 | 291 | 6.39e-12 | 65.1 |
| MS.gene041664.t1 | AT4G15090 | 27.746 | 173 | 122 | 3 | 59 | 230 | 121 | 291 | 6.39e-12 | 65.1 |
| MS.gene041664.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 62 | 230 | 107 | 262 | 6.86e-12 | 65.1 |
| MS.gene041664.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 62 | 230 | 107 | 262 | 6.86e-12 | 65.1 |
| MS.gene041664.t1 | AT1G10240 | 23.721 | 215 | 143 | 6 | 21 | 230 | 95 | 293 | 2.70e-11 | 63.2 |
| MS.gene041664.t1 | AT1G10240 | 23.721 | 215 | 143 | 6 | 21 | 230 | 95 | 293 | 2.70e-11 | 63.2 |
| MS.gene041664.t1 | AT2G27110 | 34.146 | 82 | 54 | 0 | 149 | 230 | 36 | 117 | 5.88e-11 | 62.0 |
Find 33 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 6.3:-69513659 | MS.gene041664:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.341758 | 6.3:+69513609 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | 6.3:-69513602 | None:intergenic |
| ACCGAACAAACAATAAATAT+CGG | 0.389395 | 6.3:-69513610 | MS.gene041664:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 6.3:+69514139 | None:intergenic |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6.3:-69513962 | MS.gene041664:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 6.3:-69514080 | MS.gene041664:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 6.3:-69514097 | MS.gene041664:CDS |
| CGTTATGTTTGTAACAAAGA+AGG | 0.449439 | 6.3:-69514214 | MS.gene041664:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 6.3:-69513932 | MS.gene041664:CDS |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 6.3:-69514248 | MS.gene041664:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 6.3:-69513887 | MS.gene041664:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 6.3:+69514103 | None:intergenic |
| TCTAATGACAACTCGTCAAA+AGG | 0.488137 | 6.3:-69513819 | MS.gene041664:CDS |
| CTCAAGTAGCACAAATGCTC+TGG | 0.489977 | 6.3:+69513992 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 6.3:-69514254 | MS.gene041664:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | 6.3:-69513806 | MS.gene041664:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 6.3:-69513900 | MS.gene041664:CDS |
| TGTCTTCTGCAGCATCAACT+TGG | 0.502977 | 6.3:+69513714 | None:intergenic |
| AAATGCTCTGGTTTATGTAA+TGG | 0.504865 | 6.3:+69514004 | None:intergenic |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 6.3:+69513848 | None:intergenic |
| TGTCTATAAGATATATAAGT+GGG | 0.514682 | 6.3:-69514044 | MS.gene041664:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 6.3:-69514203 | MS.gene041664:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 6.3:-69514202 | MS.gene041664:CDS |
| AATGAAGCTCTCTCGAAACC+AGG | 0.565261 | 6.3:-69513752 | MS.gene041664:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 6.3:-69513884 | MS.gene041664:CDS |
| ATGTCTATAAGATATATAAG+TGG | 0.572287 | 6.3:-69514045 | MS.gene041664:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 6.3:-69513893 | MS.gene041664:CDS |
| GACAACTCGTCAAAAGGAGA+TGG | 0.599038 | 6.3:-69513813 | MS.gene041664:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 6.3:-69513899 | MS.gene041664:CDS |
| AACTCGTCAAAAGGAGATGG+TGG | 0.603142 | 6.3:-69513810 | MS.gene041664:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 6.3:+69514154 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 6.3:-69513879 | MS.gene041664:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTCTATAAGATATATAAG+TGG | - | chr6.3:69513829-69513848 | MS.gene041664:CDS | 20.0% |
| !! | CAATTCACAAAATAAGAAAT+CGG | - | chr6.3:69513620-69513639 | MS.gene041664:CDS | 20.0% |
| !! | TGTCTATAAGATATATAAGT+GGG | - | chr6.3:69513830-69513849 | MS.gene041664:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | chr6.3:69513626-69513645 | MS.gene041664:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | chr6.3:69514264-69514283 | MS.gene041664:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | chr6.3:69513616-69513635 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | chr6.3:69513942-69513961 | MS.gene041664:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | - | chr6.3:69513672-69513691 | MS.gene041664:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | - | chr6.3:69513975-69513994 | MS.gene041664:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | - | chr6.3:69514215-69514234 | MS.gene041664:CDS | 30.0% | |
| CGTTATGTTTGTAACAAAGA+AGG | - | chr6.3:69513660-69513679 | MS.gene041664:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | chr6.3:69514268-69514287 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | chr6.3:69513761-69513780 | MS.gene041664:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | chr6.3:69513671-69513690 | MS.gene041664:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | + | chr6.3:69514143-69514162 | None:intergenic | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | + | chr6.3:69514029-69514048 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr6.3:69513873-69513892 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | chr6.3:69513794-69513813 | MS.gene041664:CDS | 30.0% |
| ! | GAATCTTCCTTTTCGATTTT+CGG | - | chr6.3:69514019-69514038 | MS.gene041664:CDS | 30.0% |
| !!! | AAGACATAGCCAGTATTTTT+TGG | - | chr6.3:69514177-69514196 | MS.gene041664:CDS | 30.0% |
| !!! | AGACATAGCCAGTATTTTTT+GGG | - | chr6.3:69514178-69514197 | MS.gene041664:CDS | 30.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr6.3:69513912-69513931 | MS.gene041664:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | chr6.3:69513760-69513779 | MS.gene041664:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | chr6.3:69513755-69513774 | MS.gene041664:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | chr6.3:69513974-69513993 | MS.gene041664:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | chr6.3:69513697-69513716 | MS.gene041664:CDS | 35.0% | |
| TCTAATGACAACTCGTCAAA+AGG | - | chr6.3:69514055-69514074 | MS.gene041664:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | chr6.3:69513738-69513757 | None:intergenic | 35.0% | |
| TCCATCTGCCCAAAAAATAC+TGG | + | chr6.3:69514189-69514208 | None:intergenic | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | chr6.3:69513711-69513730 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | - | chr6.3:69513981-69514000 | MS.gene041664:CDS | 40.0% |
| AACTCGTCAAAAGGAGATGG+TGG | - | chr6.3:69514064-69514083 | MS.gene041664:CDS | 45.0% | |
| AATGAAGCTCTCTCGAAACC+AGG | - | chr6.3:69514122-69514141 | MS.gene041664:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | + | chr6.3:69513723-69513742 | None:intergenic | 45.0% | |
| CTCAAGTAGCACAAATGCTC+TGG | + | chr6.3:69513885-69513904 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | chr6.3:69513765-69513784 | MS.gene041664:CDS | 45.0% | |
| GACAACTCGTCAAAAGGAGA+TGG | - | chr6.3:69514061-69514080 | MS.gene041664:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | + | chr6.3:69514163-69514182 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | chr6.3:69513774-69513793 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr6.3:69513777-69513796 | MS.gene041664:CDS | 45.0% |
| !!! | GCCAGTATTTTTTGGGCAGA+TGG | - | chr6.3:69514185-69514204 | MS.gene041664:CDS | 45.0% |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | - | chr6.3:69513987-69514006 | MS.gene041664:CDS | 50.0% |
| ! | CGTCAAAAGGAGATGGTGGC+TGG | - | chr6.3:69514068-69514087 | MS.gene041664:CDS | 55.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | - | chr6.3:69513990-69514009 | MS.gene041664:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | chr6.3:69513995-69514014 | MS.gene041664:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 69513603 | 69514293 | 69513603 | ID=MS.gene041664 |
| chr6.3 | mRNA | 69513603 | 69514293 | 69513603 | ID=MS.gene041664.t1;Parent=MS.gene041664 |
| chr6.3 | exon | 69513603 | 69514293 | 69513603 | ID=MS.gene041664.t1.exon1;Parent=MS.gene041664.t1 |
| chr6.3 | CDS | 69513603 | 69514293 | 69513603 | ID=cds.MS.gene041664.t1;Parent=MS.gene041664.t1 |
| Gene Sequence |
| Protein sequence |
