AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041729

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041729.t1 MTR_1g096030 98.698 461 5 1 1 460 1 461 0.0 936
MS.gene041729.t1 MTR_7g027190 33.981 412 221 14 70 458 290 673 2.72e-64 219
MS.gene041729.t1 MTR_7g074650 32.804 378 222 10 85 458 443 792 8.97e-53 190
MS.gene041729.t1 MTR_4g102790 32.506 403 232 13 74 460 286 664 3.07e-52 187
MS.gene041729.t1 MTR_3g065980 31.540 409 222 15 74 458 166 540 7.73e-51 181
MS.gene041729.t1 MTR_5g097480 31.279 438 253 15 38 458 138 544 1.17e-48 175
MS.gene041729.t1 MTR_3g089055 29.293 396 237 12 79 458 197 565 3.44e-46 168
MS.gene041729.t1 MTR_3g089055 29.293 396 237 12 79 458 202 570 4.00e-46 168
MS.gene041729.t1 MTR_1g029420 29.798 396 235 12 79 458 224 592 2.22e-45 167
MS.gene041729.t1 MTR_4g104020 29.854 412 234 15 72 459 141 521 7.96e-45 164
MS.gene041729.t1 MTR_5g094450 28.889 405 235 14 77 458 158 532 2.39e-44 163
MS.gene041729.t1 MTR_4g077760 32.242 397 225 13 76 458 189 555 1.28e-43 161
MS.gene041729.t1 MTR_3g056110 28.715 397 242 13 77 458 172 542 7.29e-43 159
MS.gene041729.t1 MTR_1g069725 30.653 398 232 15 70 457 99 462 1.87e-42 156
MS.gene041729.t1 MTR_4g133660 30.424 401 233 15 75 457 170 542 7.89e-42 156
MS.gene041729.t1 MTR_5g058860 30.676 414 241 12 70 460 109 499 4.41e-41 153
MS.gene041729.t1 MTR_4g064200 30.100 402 237 13 72 457 276 649 8.73e-41 154
MS.gene041729.t1 MTR_2g082090 28.879 464 257 17 22 458 162 579 8.98e-41 154
MS.gene041729.t1 MTR_3g072710 31.294 425 220 16 70 457 110 499 2.82e-40 151
MS.gene041729.t1 MTR_4g076020 29.457 387 216 11 85 459 89 430 4.28e-40 149
MS.gene041729.t1 MTR_2g026250 29.973 377 211 14 103 458 252 596 1.23e-37 145
MS.gene041729.t1 MTR_8g442410 29.369 412 229 17 74 458 149 525 4.64e-37 142
MS.gene041729.t1 MTR_4g064150 29.825 399 238 14 77 457 358 732 2.54e-36 142
MS.gene041729.t1 MTR_4g064120 29.262 393 236 13 77 451 251 619 3.43e-35 138
MS.gene041729.t1 MTR_4g064180 29.722 360 222 12 77 418 251 597 2.64e-34 136
MS.gene041729.t1 MTR_7g062120 27.525 396 243 14 80 458 372 740 5.97e-34 135
MS.gene041729.t1 MTR_2g097310 29.219 397 231 15 80 457 272 637 6.73e-34 135
MS.gene041729.t1 MTR_2g097467 26.150 413 259 13 61 457 271 653 6.81e-33 132
MS.gene041729.t1 MTR_2g097410 26.961 408 244 14 73 457 364 740 1.74e-32 131
MS.gene041729.t1 MTR_2g097463 26.329 395 250 11 77 457 287 654 2.05e-31 127
MS.gene041729.t1 MTR_2g097473 25.823 395 249 12 77 457 289 653 1.16e-30 125
MS.gene041729.t1 MTR_4g064160 27.735 393 244 14 80 457 316 683 5.16e-30 124
MS.gene041729.t1 MTR_2g034260 25.659 417 260 13 65 458 195 584 1.21e-29 122
MS.gene041729.t1 MTR_2g097390 27.226 393 244 13 80 457 275 640 1.77e-29 122
MS.gene041729.t1 MTR_2g097350 27.481 393 243 15 80 457 274 639 1.70e-28 119
MS.gene041729.t1 MTR_4g097080 25.558 403 246 14 79 458 132 503 4.69e-28 117
MS.gene041729.t1 MTR_2g034250 25.845 414 264 12 65 458 195 585 6.48e-28 117
MS.gene041729.t1 MTR_5g015490 27.764 407 238 15 79 458 113 490 1.48e-27 115
MS.gene041729.t1 MTR_8g093070 30.556 396 224 18 77 457 136 495 3.90e-27 114
MS.gene041729.t1 MTR_2g034280 25.581 430 242 16 65 458 188 575 5.50e-27 114
MS.gene041729.t1 MTR_3g022830 23.714 447 256 13 71 458 16 436 5.87e-27 112
MS.gene041729.t1 MTR_7g109580 26.150 413 254 14 67 460 176 556 6.05e-27 114
MS.gene041729.t1 MTR_2g097380 30.581 327 177 16 87 395 231 525 8.85e-27 113
MS.gene041729.t1 MTR_4g026485 27.047 403 221 17 81 458 270 624 2.18e-22 100
MS.gene041729.t1 MTR_0092s0100 26.434 401 235 16 77 458 369 728 1.75e-20 95.1
MS.gene041729.t1 MTR_0092s0100 26.434 401 235 16 77 458 372 731 1.80e-20 95.1
MS.gene041729.t1 MTR_6g047750 26.961 408 227 15 81 458 296 662 9.89e-20 92.4
MS.gene041729.t1 MTR_4g076140 29.018 224 150 4 71 292 39 255 2.88e-19 90.5
MS.gene041729.t1 MTR_4g076140 31.325 166 96 4 294 459 323 470 9.16e-18 85.9
MS.gene041729.t1 MTR_5g009080 27.574 272 160 9 71 335 47 288 5.08e-19 89.7
MS.gene041729.t1 MTR_5g009080 29.070 172 104 4 288 459 326 479 1.51e-18 88.2
MS.gene041729.t1 MTR_5g015950 23.385 449 252 14 76 457 22 445 1.47e-18 88.2
MS.gene041729.t1 MTR_3g053270 23.385 449 252 14 76 457 22 445 1.47e-18 88.2
MS.gene041729.t1 MTR_2g089100 21.531 418 261 12 80 457 52 442 1.59e-18 88.2
MS.gene041729.t1 MTR_7g057230 26.263 396 239 14 81 457 295 656 2.61e-18 88.2
MS.gene041729.t1 MTR_2g099110 24.932 365 239 11 111 458 105 451 8.61e-18 85.9
MS.gene041729.t1 MTR_8g020840 22.794 408 258 13 79 458 170 548 1.52e-13 73.2
MS.gene041729.t1 MTR_1g086970 23.138 376 229 16 111 460 139 480 6.98e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041729.t1 AT3G54220 34.974 386 204 12 85 457 298 649 3.25e-55 195
MS.gene041729.t1 AT1G14920 32.847 411 225 16 63 458 155 529 6.45e-54 189
MS.gene041729.t1 AT2G04890 31.915 423 252 12 45 458 18 413 7.82e-53 183
MS.gene041729.t1 AT3G03450 32.668 401 222 16 74 458 177 545 1.80e-50 180
MS.gene041729.t1 AT5G41920 32.187 407 229 12 66 459 29 401 3.69e-50 176
MS.gene041729.t1 AT2G01570 31.017 403 223 16 73 458 217 581 1.19e-49 178
MS.gene041729.t1 AT5G17490 32.117 411 224 18 64 458 145 516 1.07e-48 174
MS.gene041729.t1 AT1G21450 30.808 396 232 12 79 458 224 593 1.10e-48 176
MS.gene041729.t1 AT1G66350 31.407 398 220 16 74 458 149 506 6.69e-48 172
MS.gene041729.t1 AT5G48150 29.949 394 234 12 81 458 123 490 6.47e-47 169
MS.gene041729.t1 AT5G48150 29.949 394 234 12 81 458 123 490 6.47e-47 169
MS.gene041729.t1 AT5G48150 29.949 394 234 12 81 458 123 490 6.47e-47 169
MS.gene041729.t1 AT5G48150 29.949 394 234 12 81 458 123 490 6.47e-47 169
MS.gene041729.t1 AT1G55580 31.148 427 221 16 80 458 44 445 1.72e-46 167
MS.gene041729.t1 AT1G63100 29.457 387 227 11 99 459 264 630 1.87e-46 170
MS.gene041729.t1 AT1G63100 29.457 387 227 11 99 459 289 655 2.89e-46 170
MS.gene041729.t1 AT5G66770 32.995 394 228 14 76 458 216 584 1.90e-45 167
MS.gene041729.t1 AT4G08250 31.204 407 238 14 70 459 97 478 1.60e-42 157
MS.gene041729.t1 AT3G50650 33.244 373 214 12 98 458 193 542 8.46e-42 156
MS.gene041729.t1 AT1G50600 29.949 394 235 12 80 458 159 526 8.89e-42 155
MS.gene041729.t1 AT1G50600 29.949 394 235 12 80 458 230 597 3.17e-41 155
MS.gene041729.t1 AT1G50600 29.949 394 235 12 80 458 234 601 3.40e-41 155
MS.gene041729.t1 AT4G17230 29.902 408 227 13 75 458 153 525 2.01e-37 144
MS.gene041729.t1 AT4G17230 29.902 408 227 13 75 458 153 525 2.01e-37 144
MS.gene041729.t1 AT2G29060 27.525 396 250 13 80 460 284 657 9.29e-32 129
MS.gene041729.t1 AT1G50420 27.253 455 235 17 74 458 51 479 9.71e-32 127
MS.gene041729.t1 AT2G29060 27.525 396 250 13 80 460 321 694 1.08e-31 129
MS.gene041729.t1 AT2G37650 25.570 395 252 13 80 459 335 702 2.80e-30 124
MS.gene041729.t1 AT2G37650 25.570 395 252 13 80 459 347 714 2.82e-30 124
MS.gene041729.t1 AT2G29065 26.044 407 241 16 80 457 252 627 2.09e-29 121
MS.gene041729.t1 AT1G07530 26.463 393 250 14 80 457 396 764 2.25e-28 119
MS.gene041729.t1 AT5G59450 25.679 405 258 13 72 458 219 598 6.44e-28 117
MS.gene041729.t1 AT3G46600 26.263 396 251 13 79 459 3 372 6.57e-28 114
MS.gene041729.t1 AT3G46600 26.396 394 251 13 79 458 211 579 2.33e-27 115
MS.gene041729.t1 AT1G07520 25.250 400 252 15 80 457 154 528 3.04e-27 114
MS.gene041729.t1 AT1G07520 25.250 400 252 15 80 457 154 528 3.04e-27 114
MS.gene041729.t1 AT3G46600 26.396 394 251 13 79 458 179 547 3.55e-27 114
MS.gene041729.t1 AT1G07520 25.250 400 252 15 80 457 319 693 4.43e-27 115
MS.gene041729.t1 AT1G07520 25.250 400 252 15 80 457 318 692 4.63e-27 115
MS.gene041729.t1 AT3G60630 30.000 330 172 15 152 458 329 622 2.66e-26 112
MS.gene041729.t1 AT5G52510 25.743 404 242 14 81 458 269 640 5.45e-23 102
MS.gene041729.t1 AT4G37650 24.020 408 257 13 80 458 146 529 2.75e-22 100
MS.gene041729.t1 AT4G00150 27.520 367 208 17 105 458 233 554 3.61e-21 96.7
MS.gene041729.t1 AT4G36710 24.631 406 262 14 66 458 111 485 4.02e-20 93.2
MS.gene041729.t1 AT3G49950 23.114 411 252 13 79 457 29 407 5.65e-19 89.4

Find 59 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGGTGGTGGTGGTTTC+AGG 0.088549 1.1:+69228764 MS.gene041729:CDS
GATCAAGAGTAATTAATTTA+AGG 0.159758 1.1:+69228266 MS.gene041729:CDS
GTGGAAACAGAATCTCTTTC+AGG 0.211105 1.1:+69228131 MS.gene041729:CDS
TCTCTTTGGGATGGCAAAAT+AGG 0.239317 1.1:+69228815 MS.gene041729:CDS
AGAATCTCTTTCAGGTTAAC+TGG 0.299270 1.1:+69228139 MS.gene041729:CDS
TCTTTCAGGTTAACTGGTTT+CGG 0.304366 1.1:+69228145 MS.gene041729:CDS
GTTAGTTTCTCAAAAGGTTT+TGG 0.310158 1.1:+69228208 MS.gene041729:CDS
TGATGGAAGGGCTATCTCTT+TGG 0.326568 1.1:+69228801 MS.gene041729:CDS
CACAGCAAATCAAGCTATCT+TGG 0.326969 1.1:+69227994 MS.gene041729:CDS
GTGAGAAGTAAGTGAATTAA+TGG 0.332976 1.1:-69227716 None:intergenic
TCCATCAACATTTCATAGAA+TGG 0.366218 1.1:-69227893 None:intergenic
AGAAACTCTTCGTCGATTGT+TGG 0.372995 1.1:-69227920 None:intergenic
ATGGAGAATCATGGATTTGT+TGG 0.401767 1.1:+69228658 MS.gene041729:CDS
ACCATTCTATGAAATGTTGA+TGG 0.404027 1.1:+69227892 MS.gene041729:CDS
AGAAGGTGGTGGTGGTTTCA+GGG 0.404726 1.1:+69228765 MS.gene041729:CDS
GATGGAAGGGCTATCTCTTT+GGG 0.413184 1.1:+69228802 MS.gene041729:CDS
AGTTTCCCTCACCGGTGATT+CGG 0.424793 1.1:+69227808 MS.gene041729:CDS
GAGTTTCAAGGTTTGTTAAG+AGG 0.425739 1.1:+69228244 MS.gene041729:CDS
TTCTGTTGATGAAAACAACT+TGG 0.427192 1.1:+69227748 MS.gene041729:CDS
CTGTTTGGTAAAGATCTGTT+AGG 0.427840 1.1:-69227787 None:intergenic
CCACTTCAATTTGAGGAAGA+AGG 0.429497 1.1:+69228748 MS.gene041729:CDS
TTGAAAATAACTGACACATT+GGG 0.429946 1.1:+69228352 MS.gene041729:CDS
CACCGGTGAGGGAAACTGTT+TGG 0.439723 1.1:-69227802 None:intergenic
AAGGTTAAGAATTGAGAAGA+AGG 0.444053 1.1:+69228534 MS.gene041729:CDS
CTTGATAAGAAGGTTCTACT+AGG 0.446731 1.1:-69228436 None:intergenic
TTGCTGTGAAATGAGCAAAT+TGG 0.450939 1.1:-69227979 None:intergenic
AGGTTAGTTAGTTTCTCAAA+AGG 0.468291 1.1:+69228202 MS.gene041729:CDS
AACTCCTATGAAGGCTATGA+AGG 0.469990 1.1:+69227614 MS.gene041729:CDS
TTTGAGGAAGAAGGTGGTGG+TGG 0.477498 1.1:+69228757 MS.gene041729:CDS
AGGGTTTCAGAAAGAGATGA+TGG 0.479941 1.1:+69228784 MS.gene041729:CDS
TTCTTTCTCTATTAACTGAA+AGG 0.482856 1.1:-69227532 None:intergenic
ATTGAAAATAACTGACACAT+TGG 0.484927 1.1:+69228351 MS.gene041729:CDS
TTATTGTCCACTTCAATTTG+AGG 0.488195 1.1:+69228741 MS.gene041729:CDS
CTCAACATTGAAGATTCAAA+TGG 0.488668 1.1:+69227689 MS.gene041729:CDS
TACCAAACAGTTTCCCTCAC+CGG 0.497883 1.1:+69227800 MS.gene041729:CDS
GGAAACATGGAAAAGTAGAA+TGG 0.498264 1.1:+69228639 MS.gene041729:CDS
AGCTTCAAGAAACTGAATCT+AGG 0.507835 1.1:+69228182 MS.gene041729:CDS
AAAAGTAGAATGGAGAATCA+TGG 0.508498 1.1:+69228649 MS.gene041729:CDS
CCTTCTTCCTCAAATTGAAG+TGG 0.513172 1.1:-69228748 None:intergenic
ACTAATAGAAACTCCTATGA+AGG 0.514783 1.1:+69227605 MS.gene041729:CDS
AAATGAGCAAATTGGTAATA+CGG 0.517397 1.1:-69227971 None:intergenic
GACACGTTGAACCGAATCAC+CGG 0.538056 1.1:-69227819 None:intergenic
AACCTTTCAATACTCTCAAG+TGG 0.550308 1.1:-69228517 None:intergenic
GTCTGTGAATCTTGATAAGA+AGG 0.567154 1.1:-69228446 None:intergenic
CAATTTGAGGAAGAAGGTGG+TGG 0.571559 1.1:+69228754 MS.gene041729:CDS
GTTGTTGTTGTTGAACAAGA+AGG 0.574596 1.1:+69228403 MS.gene041729:CDS
TTGAACCGAATCACCGGTGA+GGG 0.580280 1.1:-69227813 None:intergenic
GTCGTCGCATATTTCACCGA+TGG 0.586765 1.1:+69227839 MS.gene041729:CDS
TTTCAGAAAGAGATGATGGA+AGG 0.599854 1.1:+69228788 MS.gene041729:CDS
GCTTAATAACTATGATGATG+TGG 0.611848 1.1:+69228582 MS.gene041729:CDS
AATCAATAACATGAATAGAA+CGG 0.614448 1.1:-69228045 None:intergenic
TACCACTTGAGAGTATTGAA+AGG 0.618047 1.1:+69228515 MS.gene041729:CDS
GAAAGTGATTGAATCAGTGA+AGG 0.619627 1.1:-69228094 None:intergenic
TTCAGAAAGAGATGATGGAA+GGG 0.626052 1.1:+69228789 MS.gene041729:CDS
CTTCAATTTGAGGAAGAAGG+TGG 0.626263 1.1:+69228751 MS.gene041729:CDS
GAAGGGCTATCTCTTTGGGA+TGG 0.632533 1.1:+69228806 MS.gene041729:CDS
AATAACTATGATGATGTGGA+TGG 0.639203 1.1:+69228586 MS.gene041729:CDS
AACTATGATGATGTGGATGG+TGG 0.649454 1.1:+69228589 MS.gene041729:CDS
GTTGAACCGAATCACCGGTG+AGG 0.722471 1.1:-69227814 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGAGAAAGAAAAAAGAA+GGG + chr1.1:69227542-69227561 MS.gene041729:CDS 20.0%
!! AATATGAAAAAATGGAAACA+TGG + chr1.1:69228626-69228645 MS.gene041729:CDS 20.0%
!! AATCAATAACATGAATAGAA+CGG - chr1.1:69228048-69228067 None:intergenic 20.0%
!! GATCAAGAGTAATTAATTTA+AGG + chr1.1:69228266-69228285 MS.gene041729:CDS 20.0%
!! TAATAGAGAAAGAAAAAAGA+AGG + chr1.1:69227541-69227560 MS.gene041729:CDS 20.0%
!! TTGTGCAAAATATGAAAAAA+TGG + chr1.1:69228618-69228637 MS.gene041729:CDS 20.0%
!!! ACAACTCATTTTGTTTAAAT+AGG - chr1.1:69228329-69228348 None:intergenic 20.0%
!!! ATTGATTTTGATGTTTCATA+TGG + chr1.1:69228061-69228080 MS.gene041729:CDS 20.0%
!!! ATTTTTCTCTTCTTTTTCAA+AGG - chr1.1:69228023-69228042 None:intergenic 20.0%
! ATTGAAAATAACTGACACAT+TGG + chr1.1:69228351-69228370 MS.gene041729:CDS 25.0%
! TTCTTTCTCTATTAACTGAA+AGG - chr1.1:69227535-69227554 None:intergenic 25.0%
! TTGAAAATAACTGACACATT+GGG + chr1.1:69228352-69228371 MS.gene041729:CDS 25.0%
!! AAATGAGCAAATTGGTAATA+CGG - chr1.1:69227974-69227993 None:intergenic 25.0%
!! ATGTTTCATATGGTTTTCAA+TGG + chr1.1:69228071-69228090 MS.gene041729:CDS 25.0%
!!! AACATTGTTTTTGAGTTTCA+AGG + chr1.1:69228232-69228251 MS.gene041729:CDS 25.0%
AAAAGTAGAATGGAGAATCA+TGG + chr1.1:69228649-69228668 MS.gene041729:CDS 30.0%
AAGGTTAAGAATTGAGAAGA+AGG + chr1.1:69228534-69228553 MS.gene041729:CDS 30.0%
ACCATTCTATGAAATGTTGA+TGG + chr1.1:69227892-69227911 MS.gene041729:CDS 30.0%
ACTAATAGAAACTCCTATGA+AGG + chr1.1:69227605-69227624 MS.gene041729:CDS 30.0%
CTCAACATTGAAGATTCAAA+TGG + chr1.1:69227689-69227708 MS.gene041729:CDS 30.0%
GAAGAAGAAAAATGAAACTG+TGG + chr1.1:69228288-69228307 MS.gene041729:CDS 30.0%
GTGAGAAGTAAGTGAATTAA+TGG - chr1.1:69227719-69227738 None:intergenic 30.0%
TCCATCAACATTTCATAGAA+TGG - chr1.1:69227896-69227915 None:intergenic 30.0%
TTATTGTCCACTTCAATTTG+AGG + chr1.1:69228741-69228760 MS.gene041729:CDS 30.0%
TTGTTCAACAACAACAACAA+TGG - chr1.1:69228401-69228420 None:intergenic 30.0%
! AATAACTATGATGATGTGGA+TGG + chr1.1:69228586-69228605 MS.gene041729:CDS 30.0%
! AGAATTGAGAAGAAGGTTTT+TGG + chr1.1:69228541-69228560 MS.gene041729:CDS 30.0%
! AGGTTAGTTAGTTTCTCAAA+AGG + chr1.1:69228202-69228221 MS.gene041729:CDS 30.0%
! GCTTAATAACTATGATGATG+TGG + chr1.1:69228582-69228601 MS.gene041729:CDS 30.0%
! TTCTGTTGATGAAAACAACT+TGG + chr1.1:69227748-69227767 MS.gene041729:CDS 30.0%
!! GTTAGTTTCTCAAAAGGTTT+TGG + chr1.1:69228208-69228227 MS.gene041729:CDS 30.0%
!!! AGAATGGTGATTTTTTTGTG+AGG - chr1.1:69227880-69227899 None:intergenic 30.0%
AACCTTTCAATACTCTCAAG+TGG - chr1.1:69228520-69228539 None:intergenic 35.0%
AGCTTCAAGAAACTGAATCT+AGG + chr1.1:69228182-69228201 MS.gene041729:CDS 35.0%
ATGGAGAATCATGGATTTGT+TGG + chr1.1:69228658-69228677 MS.gene041729:CDS 35.0%
CAACAACAACAACAATGGAA+GGG - chr1.1:69228396-69228415 None:intergenic 35.0%
CTGTTTGGTAAAGATCTGTT+AGG - chr1.1:69227790-69227809 None:intergenic 35.0%
CTTGATAAGAAGGTTCTACT+AGG - chr1.1:69228439-69228458 None:intergenic 35.0%
CTTTCAGAAAAAGCAACAAG+TGG + chr1.1:69228112-69228131 MS.gene041729:CDS 35.0%
GAAAGTGATTGAATCAGTGA+AGG - chr1.1:69228097-69228116 None:intergenic 35.0%
GAAGAAAAATGAAACTGTGG+CGG + chr1.1:69228291-69228310 MS.gene041729:CDS 35.0%
GGAAACATGGAAAAGTAGAA+TGG + chr1.1:69228639-69228658 MS.gene041729:CDS 35.0%
GTCTGTGAATCTTGATAAGA+AGG - chr1.1:69228449-69228468 None:intergenic 35.0%
TCAACAACAACAACAATGGA+AGG - chr1.1:69228397-69228416 None:intergenic 35.0%
TTGCTGTGAAATGAGCAAAT+TGG - chr1.1:69227982-69228001 None:intergenic 35.0%
! GAGTTTTGCAGTTAAACCAT+CGG - chr1.1:69227858-69227877 None:intergenic 35.0%
! GTTGTTGTTGTTGAACAAGA+AGG + chr1.1:69228403-69228422 MS.gene041729:CDS 35.0%
! TTTTCCTTCATAGCCTTCAT+AGG - chr1.1:69227621-69227640 None:intergenic 35.0%
!! AGAATCTCTTTCAGGTTAAC+TGG + chr1.1:69228139-69228158 MS.gene041729:CDS 35.0%
!! GAGTTTCAAGGTTTGTTAAG+AGG + chr1.1:69228244-69228263 MS.gene041729:CDS 35.0%
!! TACCACTTGAGAGTATTGAA+AGG + chr1.1:69228515-69228534 MS.gene041729:CDS 35.0%
!! TCTTTCAGGTTAACTGGTTT+CGG + chr1.1:69228145-69228164 MS.gene041729:CDS 35.0%
!! TTCAGAAAGAGATGATGGAA+GGG + chr1.1:69228789-69228808 MS.gene041729:CDS 35.0%
!! TTTCAGAAAGAGATGATGGA+AGG + chr1.1:69228788-69228807 MS.gene041729:CDS 35.0%
AACTCCTATGAAGGCTATGA+AGG + chr1.1:69227614-69227633 MS.gene041729:CDS 40.0%
AGGGTTTCAGAAAGAGATGA+TGG + chr1.1:69228784-69228803 MS.gene041729:CDS 40.0%
CACAGCAAATCAAGCTATCT+TGG + chr1.1:69227994-69228013 MS.gene041729:CDS 40.0%
CCACTTCAATTTGAGGAAGA+AGG + chr1.1:69228748-69228767 MS.gene041729:CDS 40.0%
CCTTCTTCCTCAAATTGAAG+TGG - chr1.1:69228751-69228770 None:intergenic 40.0%
CTTCAATTTGAGGAAGAAGG+TGG + chr1.1:69228751-69228770 MS.gene041729:CDS 40.0%
GTGGAAACAGAATCTCTTTC+AGG + chr1.1:69228131-69228150 MS.gene041729:CDS 40.0%
! AACTATGATGATGTGGATGG+TGG + chr1.1:69228589-69228608 MS.gene041729:CDS 40.0%
! AGAAACTCTTCGTCGATTGT+TGG - chr1.1:69227923-69227942 None:intergenic 40.0%
! TTTTGCTTACTGTGTCAGCA+TGG + chr1.1:69228839-69228858 MS.gene041729:CDS 40.0%
!! TCTCTTTGGGATGGCAAAAT+AGG + chr1.1:69228815-69228834 MS.gene041729:CDS 40.0%
CAATTTGAGGAAGAAGGTGG+TGG + chr1.1:69228754-69228773 MS.gene041729:CDS 45.0%
GATGGAAGGGCTATCTCTTT+GGG + chr1.1:69228802-69228821 MS.gene041729:CDS 45.0%
TACCAAACAGTTTCCCTCAC+CGG + chr1.1:69227800-69227819 MS.gene041729:CDS 45.0%
TGATGGAAGGGCTATCTCTT+TGG + chr1.1:69228801-69228820 MS.gene041729:CDS 45.0%
AGTTTCCCTCACCGGTGATT+CGG + chr1.1:69227808-69227827 MS.gene041729:CDS 50.0%
GACACGTTGAACCGAATCAC+CGG - chr1.1:69227822-69227841 None:intergenic 50.0%
GTCGTCGCATATTTCACCGA+TGG + chr1.1:69227839-69227858 MS.gene041729:CDS 50.0%
TTGAACCGAATCACCGGTGA+GGG - chr1.1:69227816-69227835 None:intergenic 50.0%
! GAAGGGCTATCTCTTTGGGA+TGG + chr1.1:69228806-69228825 MS.gene041729:CDS 50.0%
! TTTGAGGAAGAAGGTGGTGG+TGG + chr1.1:69228757-69228776 MS.gene041729:CDS 50.0%
!! AAGAAGGTGGTGGTGGTTTC+AGG + chr1.1:69228764-69228783 MS.gene041729:CDS 50.0%
!! AGAAGGTGGTGGTGGTTTCA+GGG + chr1.1:69228765-69228784 MS.gene041729:CDS 50.0%
CACCGGTGAGGGAAACTGTT+TGG - chr1.1:69227805-69227824 None:intergenic 55.0%
GTTGAACCGAATCACCGGTG+AGG - chr1.1:69227817-69227836 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 69227491 69228873 69227491 ID=MS.gene041729
chr1.1 mRNA 69227491 69228873 69227491 ID=MS.gene041729.t1;Parent=MS.gene041729
chr1.1 exon 69227491 69228873 69227491 ID=MS.gene041729.t1.exon1;Parent=MS.gene041729.t1
chr1.1 CDS 69227491 69228873 69227491 ID=cds.MS.gene041729.t1;Parent=MS.gene041729.t1
Gene Sequence

>MS.gene041729

ATGGAAGCTGATTCAGAAGAAGATGAGCTTCTGAATCTTAGCCTTTCAGTTAATAGAGAAAGAAAAAAGAAGGGAAAAATTATCATAAGTAGAGAAAATAACAACATGATGAGTACTAATAGAAACTCCTATGAAGGCTATGAAGGAAAAATCTTTCATCTTCTTCAAATGAGAGAACAAATGCTAAGAAAAAGCACTCTCAACATTGAAGATTCAAATGGTCTTCCATTAATTCACTTACTTCTCACAACAGCAACTTCTGTTGATGAAAACAACTTGGATTCATCTCTTGAAAACCTAACAGATCTTTACCAAACAGTTTCCCTCACCGGTGATTCGGTTCAACGTGTCGTCGCATATTTCACCGATGGTTTAACTGCAAAACTCCTCACAAAAAAATCACCATTCTATGAAATGTTGATGGAAGAACCAACAATCGACGAAGAGTTTCTCGCATTCACAGATCTCTACAGAGTTTCACCGTATTACCAATTTGCTCATTTCACAGCAAATCAAGCTATCTTGGAAGCCTTTGAAAAAGAAGAGAAAAATAACCGTTCTATTCATGTTATTGATTTTGATGTTTCATATGGTTTTCAATGGCCTTCACTGATTCAATCACTTTCAGAAAAAGCAACAAGTGGAAACAGAATCTCTTTCAGGTTAACTGGTTTCGGTAAGAATTTAAAAGAGCTTCAAGAAACTGAATCTAGGTTAGTTAGTTTCTCAAAAGGTTTTGGTAACATTGTTTTTGAGTTTCAAGGTTTGTTAAGAGGATCAAGAGTAATTAATTTAAGGAAGAAGAAAAATGAAACTGTGGCGGTTAATTTAGTTTCCTATTTAAACAAAATGAGTTGTTTATTGAAAATAACTGACACATTGGGATTTGTTCATTCTCTTAACCCTTCCATTGTTGTTGTTGTTGAACAAGAAGGTAGTAAAAATCCTAGTAGAACCTTCTTATCAAGATTCACAGACACATTGCATTATTTTGCAGCTATGTTTGATTCACTTGATGATTGTTTACCACTTGAGAGTATTGAAAGGTTAAGAATTGAGAAGAAGGTTTTTGGTAAAGAGATTAAAAGCATGCTTAATAACTATGATGATGTGGATGGTGGTGTTGATTGTGCAAAATATGAAAAAATGGAAACATGGAAAAGTAGAATGGAGAATCATGGATTTGTTGGTATGAAAATGAGTTCAAAATGTTTAATACAAGCAAAATTGTTGCTTAAGATGAGAACACATTATTGTCCACTTCAATTTGAGGAAGAAGGTGGTGGTGGTTTCAGGGTTTCAGAAAGAGATGATGGAAGGGCTATCTCTTTGGGATGGCAAAATAGGTTTTTGCTTACTGTGTCAGCATGGCAATCACTTTGA
Protein sequence

>MS.gene041729.t1

MEADSEEDELLNLSLSVNRERKKKGKIIISRENNNMMSTNRNSYEGYEGKIFHLLQMREQMLRKSTLNIEDSNGLPLIHLLLTTATSVDENNLDSSLENLTDLYQTVSLTGDSVQRVVAYFTDGLTAKLLTKKSPFYEMLMEEPTIDEEFLAFTDLYRVSPYYQFAHFTANQAILEAFEKEEKNNRSIHVIDFDVSYGFQWPSLIQSLSEKATSGNRISFRLTGFGKNLKELQETESRLVSFSKGFGNIVFEFQGLLRGSRVINLRKKKNETVAVNLVSYLNKMSCLLKITDTLGFVHSLNPSIVVVVEQEGSKNPSRTFLSRFTDTLHYFAAMFDSLDDCLPLESIERLRIEKKVFGKEIKSMLNNYDDVDGGVDCAKYEKMETWKSRMENHGFVGMKMSSKCLIQAKLLLKMRTHYCPLQFEEEGGGGFRVSERDDGRAISLGWQNRFLLTVSAWQSL