Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041858.t1 | GAU37470.1 | 89.4 | 142 | 12 | 3 | 1 | 140 | 1 | 141 | 3.40E-59 | 237.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041858.t1 | Q8LG10 | 57.9 | 140 | 41 | 6 | 7 | 140 | 22 | 149 | 1.2e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041858.t1 | A0A2Z6NLR0 | 89.4 | 142 | 12 | 3 | 1 | 140 | 1 | 141 | 2.4e-59 | 237.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene041858.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041858.t1 | MTR_4g121110 | 96.528 | 144 | 1 | 1 | 1 | 140 | 1 | 144 | 1.77e-97 | 276 |
| MS.gene041858.t1 | MTR_7g112330 | 51.754 | 114 | 48 | 4 | 29 | 140 | 21 | 129 | 8.36e-30 | 105 |
| MS.gene041858.t1 | MTR_1g016170 | 63.265 | 49 | 18 | 0 | 26 | 74 | 183 | 231 | 5.12e-17 | 75.9 |
| MS.gene041858.t1 | MTR_5g020230 | 58.000 | 50 | 18 | 1 | 20 | 66 | 109 | 158 | 4.33e-13 | 64.3 |
| MS.gene041858.t1 | MTR_4g126350 | 68.421 | 38 | 12 | 0 | 22 | 59 | 178 | 215 | 5.84e-13 | 64.7 |
| MS.gene041858.t1 | MTR_2g038720 | 76.667 | 30 | 7 | 0 | 30 | 59 | 164 | 193 | 4.15e-12 | 62.0 |
| MS.gene041858.t1 | MTR_8g077510 | 64.865 | 37 | 13 | 0 | 30 | 66 | 125 | 161 | 4.89e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041858.t1 | AT3G06740 | 57.857 | 140 | 41 | 6 | 7 | 140 | 22 | 149 | 8.25e-42 | 136 |
| MS.gene041858.t1 | AT5G49300 | 53.691 | 149 | 49 | 6 | 2 | 140 | 1 | 139 | 1.60e-40 | 132 |
| MS.gene041858.t1 | AT3G16870 | 42.949 | 156 | 43 | 4 | 27 | 140 | 39 | 190 | 2.47e-29 | 105 |
| MS.gene041858.t1 | AT4G16141 | 44.444 | 162 | 36 | 6 | 29 | 136 | 36 | 197 | 5.13e-27 | 100 |
| MS.gene041858.t1 | AT5G26930 | 67.442 | 43 | 13 | 1 | 30 | 71 | 26 | 68 | 1.66e-16 | 70.9 |
| MS.gene041858.t1 | AT5G56860 | 70.732 | 41 | 12 | 0 | 23 | 63 | 223 | 263 | 3.95e-15 | 71.2 |
| MS.gene041858.t1 | AT2G18380 | 67.568 | 37 | 12 | 0 | 30 | 66 | 92 | 128 | 5.18e-13 | 63.5 |
| MS.gene041858.t1 | AT4G26150 | 77.419 | 31 | 7 | 0 | 30 | 60 | 199 | 229 | 5.26e-13 | 65.1 |
| MS.gene041858.t1 | AT4G36620 | 67.568 | 37 | 12 | 0 | 30 | 66 | 75 | 111 | 6.92e-13 | 63.5 |
| MS.gene041858.t1 | AT3G50870 | 67.568 | 37 | 12 | 0 | 30 | 66 | 152 | 188 | 2.78e-12 | 62.8 |
Find 0 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGTTCATTTTTCATTTTC+AGG | + | 32472:11476-11495 | MS.gene041858:intron | 20.0% |
| ! | AAGCAGAGATTATTGAATTT+AGG | + | 32472:11856-11875 | MS.gene041858:CDS | 25.0% |
| ! | AGCAGAGATTATTGAATTTA+GGG | + | 32472:11857-11876 | MS.gene041858:CDS | 25.0% |
| ! | GATTAGTGATTAATTACCTT+TGG | - | 32472:11624-11643 | MS.gene041858:intergenic | 25.0% |
| !! | ATTGTTGTTATCTGATGAAA+CGG | - | 32472:11535-11554 | MS.gene041858:intergenic | 25.0% |
| TGTTTATGAATCGATCACAT+TGG | + | 32472:11887-11906 | MS.gene041858:CDS | 30.0% | |
| TTGTTGATGTCTCTTTCTTA+TGG | + | 32472:11943-11962 | MS.gene041858:CDS | 30.0% | |
| !!! | TTTCATTTTCAGGTATCAGA+AGG | + | 32472:11486-11505 | MS.gene041858:intron | 30.0% |
| AACGAAGAAGGAACAAGAAA+GGG | + | 32472:11784-11803 | MS.gene041858:CDS | 35.0% | |
| AAGAAGAAGAGAGCGATATT+AGG | + | 32472:11745-11764 | MS.gene041858:CDS | 35.0% | |
| AGAAGAAGAGAGCGATATTA+GGG | + | 32472:11746-11765 | MS.gene041858:CDS | 35.0% | |
| CGATCACATTGGAAGAAATT+GGG | + | 32472:11898-11917 | MS.gene041858:CDS | 35.0% | |
| TCGATCACATTGGAAGAAAT+TGG | + | 32472:11897-11916 | MS.gene041858:CDS | 35.0% | |
| TTGAAGTGAAGTGTGTGATT+AGG | - | 32472:11415-11434 | MS.gene041858:intergenic | 35.0% | |
| AAGGGGAAAAAGAGTAACAG+TGG | + | 32472:11802-11821 | MS.gene041858:CDS | 40.0% | |
| ACGAAGAAGGAACAAGAAAG+GGG | + | 32472:11785-11804 | MS.gene041858:CDS | 40.0% | |
| AGCAAAGGAAACAACGAAGA+AGG | + | 32472:11772-11791 | MS.gene041858:CDS | 40.0% | |
| CAACGAAGAAGGAACAAGAA+AGG | + | 32472:11783-11802 | MS.gene041858:CDS | 40.0% | |
| GAACTTCCAAAACTCCTCTA+TGG | + | 32472:11580-11599 | MS.gene041858:CDS | 40.0% | |
| GCGATATTAGGGATAAGCAA+AGG | + | 32472:11757-11776 | MS.gene041858:CDS | 40.0% | |
| GTTGTTATCTGATGAAACGG+CGG | - | 32472:11532-11551 | MS.gene041858:intergenic | 40.0% | |
| TTAATTACCTTTGGTCCTGC+AGG | - | 32472:11615-11634 | MS.gene041858:intergenic | 40.0% | |
| ! | CAATCAGCACAGGTTTTCTT+TGG | - | 32472:11558-11577 | MS.gene041858:intergenic | 40.0% |
| !! | AAGAAAACCTGTGCTGATTG+CGG | + | 32472:11558-11577 | MS.gene041858:CDS | 40.0% |
| GGGAAAAAGAGTAACAGTGG+CGG | + | 32472:11805-11824 | MS.gene041858:CDS | 45.0% | |
| GGTGGTTGATCACAGTGATA+AGG | + | 32472:11359-11378 | MS.gene041858:CDS | 45.0% | |
| AGTCACTGTGCAATGCTTGC+GGG | + | 32472:11710-11729 | MS.gene041858:intron | 50.0% | |
| CAGTCACTGTGCAATGCTTG+CGG | + | 32472:11709-11728 | MS.gene041858:intron | 50.0% | |
| TCCAAAACTCCTCTATGGCG+CGG | + | 32472:11585-11604 | MS.gene041858:CDS | 50.0% | |
| ! | AACAGTGGCGGAAGCAAAGT+TGG | + | 32472:11817-11836 | MS.gene041858:CDS | 50.0% |
| AAAACTCCTCTATGGCGCGG+TGG | + | 32472:11588-11607 | MS.gene041858:CDS | 55.0% | |
| CTGATGAAACGGCGGCGTTA+GGG | - | 32472:11524-11543 | MS.gene041858:intergenic | 55.0% | |
| GGAAGTTCCGCAATCAGCAC+AGG | - | 32472:11568-11587 | MS.gene041858:intergenic | 55.0% | |
| TCTGATGAAACGGCGGCGTT+AGG | - | 32472:11525-11544 | MS.gene041858:intergenic | 55.0% | |
| TGTGCAATGCTTGCGGGATC+AGG | + | 32472:11716-11735 | MS.gene041858:CDS | 55.0% | |
| ! | ACCGCGCCATAGAGGAGTTT+TGG | - | 32472:11589-11608 | MS.gene041858:intergenic | 55.0% |
| ATGCTTGCGGGATCAGGAGC+AGG | + | 32472:11722-11741 | MS.gene041858:CDS | 60.0% | |
| CGGTGGTCCTGCAGGACCAA+AGG | + | 32472:11605-11624 | MS.gene041858:CDS | 65.0% | |
| CTATGGCGCGGTGGTCCTGC+AGG | + | 32472:11597-11616 | MS.gene041858:CDS | 70.0% | |
| GCAGGACCACCGCGCCATAG+AGG | - | 32472:11597-11616 | MS.gene041858:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32472 | gene | 11357 | 11981 | 11357 | ID=MS.gene041858 |
| 32472 | mRNA | 11357 | 11981 | 11357 | ID=MS.gene041858.t1;Parent=MS.gene041858 |
| 32472 | exon | 11357 | 11380 | 11357 | ID=MS.gene041858.t1.exon1;Parent=MS.gene041858.t1 |
| 32472 | CDS | 11357 | 11380 | 11357 | ID=cds.MS.gene041858.t1;Parent=MS.gene041858.t1 |
| 32472 | exon | 11498 | 11626 | 11498 | ID=MS.gene041858.t1.exon2;Parent=MS.gene041858.t1 |
| 32472 | CDS | 11498 | 11626 | 11498 | ID=cds.MS.gene041858.t1;Parent=MS.gene041858.t1 |
| 32472 | exon | 11712 | 11981 | 11712 | ID=MS.gene041858.t1.exon3;Parent=MS.gene041858.t1 |
| 32472 | CDS | 11712 | 11981 | 11712 | ID=cds.MS.gene041858.t1;Parent=MS.gene041858.t1 |
| Gene Sequence |
| Protein sequence |
