AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042263

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042263.t1 MTR_4g131600 90.986 588 42 1 1 577 3 590 0.0 1063
MS.gene042263.t1 MTR_4g131570 70.451 599 151 4 2 577 1 596 0.0 822
MS.gene042263.t1 MTR_4g131580 69.831 590 159 4 2 572 1 590 0.0 799
MS.gene042263.t1 MTR_2g450070 40.545 661 289 19 1 568 1 650 1.71e-123 379
MS.gene042263.t1 MTR_2g450070 40.545 661 289 19 1 568 1 650 4.56e-123 378
MS.gene042263.t1 MTR_3g106220 52.147 326 140 6 15 324 5 330 1.17e-92 298
MS.gene042263.t1 MTR_1g013170 48.378 339 144 6 14 324 1 336 6.48e-92 291
MS.gene042263.t1 MTR_1g032570 55.200 250 102 5 18 258 11 259 8.02e-84 274
MS.gene042263.t1 MTR_4g121020 44.959 367 166 10 15 351 25 385 4.02e-81 268
MS.gene042263.t1 MTR_4g121020 44.959 367 166 10 15 351 25 385 4.19e-81 268
MS.gene042263.t1 MTR_1g013180 48.000 325 141 6 14 313 152 473 1.06e-80 267
MS.gene042263.t1 MTR_3g086100 54.582 251 101 5 18 259 13 259 2.50e-80 263
MS.gene042263.t1 MTR_8g079940 49.254 268 120 5 16 278 23 279 1.05e-77 255
MS.gene042263.t1 MTR_7g117705 46.254 307 158 4 14 313 1 307 2.51e-77 252
MS.gene042263.t1 MTR_2g034960 43.353 346 184 4 12 351 23 362 7.48e-77 255
MS.gene042263.t1 MTR_3g102590 50.000 236 70 3 18 252 8 196 2.59e-60 200
MS.gene042263.t1 MTR_1g032570 54.974 191 76 5 77 258 1 190 6.18e-58 203
MS.gene042263.t1 MTR_3g102590 50.000 232 68 3 18 248 8 192 1.19e-57 192
MS.gene042263.t1 MTR_3g086100 51.042 192 81 5 77 259 1 188 9.99e-51 182
MS.gene042263.t1 MTR_4g021760 37.872 235 123 4 17 251 10 221 3.75e-49 173
MS.gene042263.t1 MTR_4g021790 37.500 256 131 4 18 273 10 236 2.75e-47 167
MS.gene042263.t1 MTR_3g102600 60.769 130 51 0 12 141 2 131 1.43e-46 162
MS.gene042263.t1 MTR_3g102600 60.377 53 21 0 198 250 131 183 4.97e-15 74.3
MS.gene042263.t1 MTR_3g102600 60.769 130 51 0 12 141 2 131 2.05e-46 163
MS.gene042263.t1 MTR_3g102600 42.056 107 45 2 198 304 131 220 7.49e-18 83.2
MS.gene042263.t1 MTR_4g021855 38.235 238 124 3 18 255 10 224 1.57e-45 163
MS.gene042263.t1 MTR_4g021845 38.627 233 118 5 17 248 9 217 1.33e-44 158
MS.gene042263.t1 MTR_6g045327 35.000 260 136 6 12 262 8 243 6.57e-42 155
MS.gene042263.t1 MTR_4g121020 37.943 282 142 9 97 351 6 281 1.63e-39 152
MS.gene042263.t1 MTR_0450s0040 36.364 242 136 6 22 263 8 231 2.51e-39 147
MS.gene042263.t1 MTR_1g013160 48.344 151 73 2 80 225 1 151 4.57e-36 132
MS.gene042263.t1 MTR_8g105600 35.417 240 150 3 14 252 5 240 8.70e-34 136
MS.gene042263.t1 MTR_5g055260 34.043 235 103 8 18 251 10 193 1.87e-31 123
MS.gene042263.t1 MTR_3g102600 63.415 82 30 0 60 141 1 82 1.40e-30 117
MS.gene042263.t1 MTR_3g102600 60.377 53 21 0 198 250 82 134 2.78e-15 73.6
MS.gene042263.t1 MTR_3g102600 63.415 82 30 0 60 141 1 82 1.56e-30 118
MS.gene042263.t1 MTR_7g026400 35.096 208 124 3 48 255 17 213 2.89e-30 125
MS.gene042263.t1 MTR_8g032710 30.502 259 153 7 17 251 16 271 4.11e-28 118
MS.gene042263.t1 MTR_3g092780 32.979 188 105 5 22 195 56 236 4.94e-24 107
MS.gene042263.t1 MTR_3g092780 32.979 188 105 5 22 195 56 236 5.13e-24 107
MS.gene042263.t1 MTR_8g093040 28.926 242 134 3 14 255 10 213 1.70e-23 100
MS.gene042263.t1 MTR_3g092780 32.240 183 112 4 22 195 56 235 3.05e-23 104
MS.gene042263.t1 MTR_3g092780 32.240 183 112 4 22 195 56 235 3.29e-23 104
MS.gene042263.t1 MTR_4g023980 31.875 160 85 3 64 222 35 171 2.53e-22 94.7
MS.gene042263.t1 MTR_7g118260 36.090 133 76 2 19 145 11 140 1.29e-21 99.4
MS.gene042263.t1 MTR_6g016850 33.121 157 102 2 14 168 5 160 1.34e-21 92.4
MS.gene042263.t1 MTR_7g118260 36.719 128 72 2 19 140 11 135 2.66e-21 98.2
MS.gene042263.t1 MTR_4g061360 37.705 122 73 1 22 140 89 210 1.84e-19 93.2
MS.gene042263.t1 MTR_4g064730 52.381 84 37 2 175 255 104 187 1.43e-18 87.4
MS.gene042263.t1 MTR_1g067110 34.426 122 77 1 22 140 85 206 2.65e-18 89.4
MS.gene042263.t1 MTR_1g067110 34.426 122 77 1 22 140 85 206 2.85e-18 89.4
MS.gene042263.t1 MTR_3g037390 31.579 133 86 2 14 141 14 146 3.65e-18 88.6
MS.gene042263.t1 MTR_8g024260 33.600 125 80 1 24 145 38 162 1.20e-17 87.0
MS.gene042263.t1 MTR_4g108880 30.303 132 87 2 15 141 24 155 6.21e-17 84.7
MS.gene042263.t1 MTR_7g089010 58.621 58 24 0 198 255 90 147 2.51e-16 80.5
MS.gene042263.t1 MTR_5g014040 33.553 152 84 3 152 294 264 407 1.65e-15 80.1
MS.gene042263.t1 MTR_5g014040 38.400 125 71 3 18 140 14 134 2.43e-15 79.3
MS.gene042263.t1 MTR_4g098870 48.750 80 31 1 192 261 303 382 2.08e-15 79.7
MS.gene042263.t1 MTR_4g098870 34.677 124 77 2 18 140 14 134 5.87e-14 75.1
MS.gene042263.t1 MTR_4g098870 48.750 80 31 1 192 261 323 402 2.45e-15 79.7
MS.gene042263.t1 MTR_4g098870 34.677 124 77 2 18 140 34 154 7.72e-14 74.7
MS.gene042263.t1 MTR_4g098870 48.750 80 31 1 192 261 303 382 2.58e-15 79.3
MS.gene042263.t1 MTR_4g098870 34.677 124 77 2 18 140 14 134 4.06e-14 75.5
MS.gene042263.t1 MTR_5g014040 38.400 125 71 3 18 140 14 134 2.59e-15 79.3
MS.gene042263.t1 MTR_5g014040 32.484 157 84 4 152 294 264 412 1.50e-13 73.9
MS.gene042263.t1 MTR_4g098870 48.750 80 31 1 192 261 323 402 2.77e-15 79.3
MS.gene042263.t1 MTR_4g098870 34.677 124 77 2 18 140 34 154 5.54e-14 75.1
MS.gene042263.t1 MTR_4g098870 48.750 80 31 1 192 261 342 421 2.93e-15 79.3
MS.gene042263.t1 MTR_4g098870 34.677 124 77 2 18 140 53 173 5.98e-14 75.1
MS.gene042263.t1 MTR_7g115530 62.500 56 20 1 197 251 216 271 7.67e-14 72.8
MS.gene042263.t1 MTR_5g014040 32.484 157 84 4 152 294 224 372 1.13e-13 73.9
MS.gene042263.t1 MTR_7g115530 62.500 56 20 1 197 251 216 271 1.34e-13 72.4
MS.gene042263.t1 MTR_7g115530 62.500 56 20 1 197 251 216 271 1.45e-13 72.8
MS.gene042263.t1 MTR_7g115530 62.500 56 20 1 197 251 216 271 2.43e-13 72.8
MS.gene042263.t1 MTR_4g078810 42.857 84 46 1 197 280 156 237 2.80e-13 72.4
MS.gene042263.t1 MTR_2g090675 36.585 123 52 4 135 257 123 219 3.68e-13 72.0
MS.gene042263.t1 MTR_1g080330 60.714 56 21 1 197 251 173 228 4.33e-13 70.5
MS.gene042263.t1 MTR_1g080330 60.714 56 21 1 197 251 173 228 6.40e-13 71.2
MS.gene042263.t1 MTR_6g088950 30.827 133 82 3 48 176 34 160 2.25e-12 67.0
MS.gene042263.t1 MTR_7g115530 61.111 54 20 1 197 249 216 269 4.65e-12 67.0
MS.gene042263.t1 MTR_7g098250 48.000 75 37 2 199 271 262 336 5.00e-12 68.9
MS.gene042263.t1 MTR_7g490310 28.662 157 89 3 14 149 1 155 5.04e-12 66.6
MS.gene042263.t1 MTR_4g081710 49.153 59 29 1 195 252 13 71 5.70e-12 67.8
MS.gene042263.t1 MTR_2g027860 55.000 60 25 2 199 257 49 107 6.50e-12 67.4
MS.gene042263.t1 MTR_6g032990 54.237 59 26 1 195 252 38 96 8.39e-12 67.8
MS.gene042263.t1 MTR_7g115530 60.000 55 21 1 197 250 216 270 1.11e-11 66.2
MS.gene042263.t1 MTR_2g023580 60.000 55 21 1 198 251 54 108 1.62e-11 65.1
MS.gene042263.t1 MTR_5g041350 50.847 59 28 1 195 252 11 69 1.65e-11 66.6
MS.gene042263.t1 MTR_1g093080 44.928 69 36 2 188 255 201 268 2.00e-11 66.2
MS.gene042263.t1 MTR_4g086835 48.276 58 30 0 198 255 273 330 2.25e-11 66.6
MS.gene042263.t1 MTR_2g086450 50.847 59 28 1 195 252 41 99 4.43e-11 65.5
MS.gene042263.t1 MTR_3g450310 49.296 71 29 2 188 251 2 72 5.51e-11 62.8
MS.gene042263.t1 MTR_4g113140 31.250 112 70 3 145 255 187 292 5.59e-11 65.5
MS.gene042263.t1 MTR_0223s0040 54.545 66 29 1 198 262 17 82 7.86e-11 63.5
MS.gene042263.t1 MTR_5g017980 46.552 58 31 0 198 255 191 248 8.44e-11 63.9
MS.gene042263.t1 MTR_5g017980 46.552 58 31 0 198 255 192 249 8.82e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042263.t1 AT2G25180 46.486 370 170 7 18 362 13 379 1.41e-89 288
MS.gene042263.t1 AT4G31920 50.000 318 122 7 18 311 13 317 8.49e-81 264
MS.gene042263.t1 AT1G67710 55.078 256 105 5 17 265 5 257 5.43e-80 261
MS.gene042263.t1 AT5G58080 48.466 326 144 9 8 314 3 323 6.16e-80 264
MS.gene042263.t1 AT3G16857 47.039 304 136 3 9 288 24 326 6.81e-80 265
MS.gene042263.t1 AT3G16857 44.311 334 157 4 9 314 24 356 1.30e-79 265
MS.gene042263.t1 AT2G01760 50.195 257 112 4 18 260 7 261 1.30e-69 229
MS.gene042263.t1 AT2G01760 45.263 285 112 5 18 260 7 289 1.68e-63 214
MS.gene042263.t1 AT3G16857 43.706 286 132 4 57 314 2 286 2.93e-63 219
MS.gene042263.t1 AT5G49240 41.633 245 131 5 12 248 32 272 8.56e-53 182
MS.gene042263.t1 AT1G67710 52.308 195 84 4 77 265 1 192 6.83e-52 184
MS.gene042263.t1 AT2G01760 48.990 198 85 4 77 260 1 196 2.20e-46 166
MS.gene042263.t1 AT3G62670 39.662 237 125 6 23 251 42 268 2.13e-39 149
MS.gene042263.t1 AT1G49190 30.667 300 168 8 18 291 30 315 3.29e-39 148
MS.gene042263.t1 AT1G67710 47.701 174 82 4 98 265 13 183 1.38e-37 145
MS.gene042263.t1 AT5G07210 29.965 287 155 5 22 267 16 297 8.06e-32 130
MS.gene042263.t1 AT5G07210 29.153 295 155 6 22 267 16 305 2.08e-29 123
MS.gene042263.t1 AT2G27070 29.965 287 158 8 22 267 16 300 1.74e-28 120
MS.gene042263.t1 AT1G49190 36.800 125 79 0 18 142 30 154 8.23e-22 100
MS.gene042263.t1 AT2G46790 38.211 123 71 2 22 140 37 158 1.27e-21 98.2
MS.gene042263.t1 AT2G46790 38.211 123 71 2 22 140 37 158 1.43e-21 98.6
MS.gene042263.t1 AT5G24470 36.000 125 75 2 21 141 49 172 1.54e-21 99.0
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 2.34e-19 91.3
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 2.34e-19 91.3
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 2.34e-19 91.3
MS.gene042263.t1 AT1G68210 24.444 270 170 4 22 258 13 281 2.41e-19 92.8
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 5.11e-19 90.9
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 5.11e-19 90.9
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 5.11e-19 90.9
MS.gene042263.t1 AT5G60100 38.525 122 72 1 22 140 64 185 7.23e-19 90.5
MS.gene042263.t1 AT5G05090 50.667 75 37 0 181 255 63 137 1.21e-17 83.6
MS.gene042263.t1 AT5G59570 39.623 106 62 1 152 257 96 199 1.47e-17 84.0
MS.gene042263.t1 AT5G59570 39.623 106 62 1 152 257 96 199 1.47e-17 84.0
MS.gene042263.t1 AT3G46640 58.333 60 25 0 198 257 143 202 2.29e-17 84.0
MS.gene042263.t1 AT3G46640 58.333 60 25 0 198 257 143 202 2.29e-17 84.0
MS.gene042263.t1 AT3G46640 60.345 58 23 0 198 255 143 200 3.52e-17 83.2
MS.gene042263.t1 AT5G02810 34.426 122 77 1 22 140 78 199 3.84e-17 85.5
MS.gene042263.t1 AT2G40970 58.621 58 24 0 198 255 104 161 4.77e-17 81.6
MS.gene042263.t1 AT3G10760 55.385 65 29 0 191 255 97 161 5.75e-17 82.8
MS.gene042263.t1 AT5G61380 31.818 132 85 2 15 141 10 141 8.51e-17 84.3
MS.gene042263.t1 AT5G44190 61.818 55 21 0 197 251 145 199 1.67e-15 78.6
MS.gene042263.t1 AT5G44190 61.818 55 21 0 197 251 145 199 1.76e-15 79.0
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 1.53e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 1.53e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 1.53e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 1.53e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 1.53e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 1.53e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 2.03e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 2.03e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 2.03e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 2.03e-13 73.6
MS.gene042263.t1 AT4G18020 34.266 143 85 3 16 151 12 152 2.03e-13 73.6
MS.gene042263.t1 AT2G20570 54.098 61 28 0 197 257 151 211 7.52e-13 71.2
MS.gene042263.t1 AT5G45580 50.000 70 32 2 194 262 4 71 1.21e-12 68.9
MS.gene042263.t1 AT5G45580 50.000 70 32 2 194 262 20 87 1.25e-12 68.9
MS.gene042263.t1 AT2G20570 59.259 54 22 0 204 257 174 227 3.03e-12 69.3
MS.gene042263.t1 AT2G01060 55.000 60 25 2 199 257 15 73 5.23e-12 67.4
MS.gene042263.t1 AT4G37180 51.667 60 28 1 197 255 208 267 3.04e-11 65.9
MS.gene042263.t1 AT3G04030 52.727 55 25 1 199 252 45 99 3.18e-11 65.9
MS.gene042263.t1 AT3G04030 52.727 55 25 1 199 252 45 99 3.70e-11 65.9
MS.gene042263.t1 AT4G37180 51.667 60 28 1 197 255 215 274 3.72e-11 65.5
MS.gene042263.t1 AT5G06800 50.000 66 31 2 199 263 192 256 4.86e-11 64.3
MS.gene042263.t1 AT5G18240 54.545 55 24 1 199 252 45 99 5.47e-11 65.1
MS.gene042263.t1 AT5G18240 54.545 55 24 1 199 252 45 99 5.47e-11 65.1
MS.gene042263.t1 AT5G18240 54.545 55 24 1 199 252 45 99 5.81e-11 65.1
MS.gene042263.t1 AT5G18240 54.545 55 24 1 199 252 45 99 5.81e-11 65.1
MS.gene042263.t1 AT5G18240 54.545 55 24 1 199 252 45 99 6.18e-11 65.1

Find 113 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATTGGTGTCTCGAAAT+TGG 0.196977 8.4:-1943897 None:intergenic
GATTATCTTGCTTCTTCTAA+TGG 0.232806 8.4:+1944454 MS.gene042263:CDS
CCACTTCTGACAGGGTTAAA+AGG 0.242635 8.4:-1944196 None:intergenic
GGACTTAAATTAATGTTATC+AGG 0.249835 8.4:-1944001 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.252390 8.4:+1943746 MS.gene042263:CDS
AAAAGAAGCCTCGTTTAGTT+TGG 0.279239 8.4:+1943244 MS.gene042263:CDS
CAATGTCAAGATGGCTTACT+TGG 0.323761 8.4:+1944364 MS.gene042263:CDS
TGATTAATGAACTATTAGTT+TGG 0.331944 8.4:-1944507 None:intergenic
GAGCAATTGCTTAATGTAGA+TGG 0.338130 8.4:+1944601 MS.gene042263:CDS
CCCGGCGGGTATGCGTGTTC+TGG 0.339385 8.4:+1940849 MS.gene042263:CDS
CTCACTCAATGGAACAGAAT+TGG 0.343212 8.4:-1944236 None:intergenic
ATCATTAGTGATGGGGTATA+TGG 0.343848 8.4:+1943105 MS.gene042263:CDS
AAAGAAGCCTCGTTTAGTTT+GGG 0.355798 8.4:+1943245 MS.gene042263:CDS
AGACATCGTTGCTGTTCCTT+TGG 0.355803 8.4:+1944330 MS.gene042263:CDS
CATTCCTTCCAAGTTCAAAC+TGG 0.363583 8.4:-1943831 None:intergenic
GATAAATATTCTGCTGAAAC+TGG 0.368030 8.4:-1943976 None:intergenic
CACACCATCCAGTTTGAACT+TGG 0.369083 8.4:+1943823 MS.gene042263:CDS
CTTCTCACATGCAACTATAA+TGG 0.375217 8.4:+1940916 MS.gene042263:CDS
GTTTCTGCAGTTAATCTTGT+CGG 0.381222 8.4:+1943291 MS.gene042263:CDS
TATCAAATGGCTATCTAAAC+AGG 0.381598 8.4:+1943688 MS.gene042263:CDS
CATCGTTGCTGTTCCTTTGG+AGG 0.383670 8.4:+1944333 MS.gene042263:CDS
AACATTGGTGTCTCGAAATT+GGG 0.390536 8.4:-1943896 None:intergenic
ATAAATCACTCTCCTGTTGA+AGG 0.396905 8.4:+1944154 MS.gene042263:CDS
GATTAATGAACTATTAGTTT+GGG 0.399970 8.4:-1944506 None:intergenic
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8.4:+1942819 MS.gene042263:CDS
TCCAGCACTTTGAGATAAGT+TGG 0.404451 8.4:-1940886 None:intergenic
AAATGTATCCAGCCATTTGC+AGG 0.408981 8.4:+1943488 MS.gene042263:CDS
ACTGCATTTGAATCATCAAT+TGG 0.413674 8.4:-1944022 None:intergenic
TCAAGTTGAGAAGTTCTATT+CGG 0.435476 8.4:+1944540 MS.gene042263:CDS
ATATAATTTGTACCTGCAAA+TGG 0.440246 8.4:-1943500 None:intergenic
TTCACAGCAGAACCTTCAAC+AGG 0.443727 8.4:-1944166 None:intergenic
TGAATTGTTGAGAGAAAAGA+AGG 0.450895 8.4:+1942735 MS.gene042263:CDS
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8.4:-1942793 None:intergenic
GAGAAGTTCTATTCGGATCA+AGG 0.456955 8.4:+1944547 MS.gene042263:CDS
ATTCTAGTTCATGATGGTTG+TGG 0.457111 8.4:+1940786 None:intergenic
ACATGGCATTCAAACGTCAA+TGG 0.462461 8.4:+1943946 MS.gene042263:CDS
AGAATTGGAAGCAAAGTTAG+AGG 0.465879 8.4:-1944221 None:intergenic
AGGGTTCAGTAGGTGGATAT+GGG 0.469935 8.4:+1943747 MS.gene042263:CDS
CATTGCATCGTTTCCTCCAA+AGG 0.471428 8.4:-1944346 None:intergenic
TACTACAACTACGAAGTCAT+TGG 0.471545 8.4:+1942705 MS.gene042263:CDS
GATACAGAATTGATATCAAA+TGG 0.472061 8.4:+1943675 MS.gene042263:CDS
TGACAGTGGCTCTGATGTCT+TGG 0.472341 8.4:-1944063 None:intergenic
CTTGACTTGATGAATGTTGA+AGG 0.474751 8.4:+1943453 MS.gene042263:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8.4:-1943024 None:intergenic
CAAGGAATCATGGAATCAAA+TGG 0.482889 8.4:+1944565 MS.gene042263:CDS
TTCAGAATAGTCGTGTGCCT+TGG 0.483059 8.4:+1944629 MS.gene042263:CDS
TTCCTCCTTCAACAATACCT+TGG 0.487555 8.4:+1944432 MS.gene042263:CDS
AATTTGTACCTGCAAATGGC+TGG 0.488638 8.4:-1943496 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8.4:+1943034 MS.gene042263:CDS
GTTGGATCCCTACCAGCAAA+AGG 0.495630 8.4:+1943727 MS.gene042263:CDS
CAGCCGACAAAACATGTCAT+TGG 0.496819 8.4:-1943798 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+1943463 MS.gene042263:CDS
TTGTTAAAATCCTCACTCAA+TGG 0.497939 8.4:-1944247 None:intergenic
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8.4:+1943068 MS.gene042263:CDS
TTAGAGGAACCACTTCTGAC+AGG 0.501081 8.4:-1944205 None:intergenic
ATAATCCAAGGTATTGTTGA+AGG 0.508220 8.4:-1944437 None:intergenic
ATCAAGGTATCATTAGTGAT+GGG 0.510346 8.4:+1943097 MS.gene042263:CDS
AATCAAGGTATCATTAGTGA+TGG 0.511362 8.4:+1943096 MS.gene042263:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8.4:+1942936 MS.gene042263:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.4:+1943081 MS.gene042263:CDS
CCATCCAGTTTGAACTTGGA+AGG 0.517311 8.4:+1943827 MS.gene042263:CDS
AATGCAGTTACAGATTCCTC+TGG 0.518088 8.4:+1944037 MS.gene042263:CDS
CTTTCACTTTCAGAAAACAT+TGG 0.525109 8.4:-1943911 None:intergenic
TCCAACTTATCTCAAAGTGC+TGG 0.528445 8.4:+1940885 MS.gene042263:CDS
TGGCTATCTAAACAGGATAG+AGG 0.531559 8.4:+1943695 MS.gene042263:CDS
CAATTCTGTTCCATTGAGTG+AGG 0.541688 8.4:+1944237 MS.gene042263:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8.4:-1942768 None:intergenic
TTCACTTATTGTGTTGTCCA+AGG 0.543954 8.4:-1944646 None:intergenic
CTATTCGGATCAAGGAATCA+TGG 0.544673 8.4:+1944555 MS.gene042263:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.548262 8.4:+1943740 MS.gene042263:CDS
GCCAGAACACGCATACCCGC+CGG 0.553676 8.4:-1940850 None:intergenic
ATGGTTGTGGAGGATAGTTT+CGG 0.554649 8.4:+1940799 MS.gene042263:CDS
CCTTCCAAGTTCAAACTGGA+TGG 0.554980 8.4:-1943827 None:intergenic
TTAGGAGAAGGTTTCCCGGC+GGG 0.561505 8.4:+1940835 MS.gene042263:CDS
ATGTCATTGGATGCATATGC+TGG 0.565239 8.4:-1943785 None:intergenic
TAATGGAGACACTAGTTCCA+TGG 0.565315 8.4:+1944471 MS.gene042263:CDS
CCAGAACACGCATACCCGCC+GGG 0.571213 8.4:-1940849 None:intergenic
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8.4:-1942816 None:intergenic
TTGGATCCCTACCAGCAAAA+GGG 0.576563 8.4:+1943728 MS.gene042263:CDS
TTGACTTGATGAATGTTGAA+GGG 0.580486 8.4:+1943454 MS.gene042263:CDS
GAAACGATGCAATGTCAAGA+TGG 0.582127 8.4:+1944355 MS.gene042263:CDS
GGATAGAGGAGATGATGCGT+TGG 0.582696 8.4:+1943709 MS.gene042263:CDS
TTGGGCTAAACTTTGTACCA+TGG 0.585108 8.4:-1944488 None:intergenic
AAGAAAGGTACAAAGTGAGG+AGG 0.585901 8.4:+1943182 MS.gene042263:CDS
GAAAGTTCGAGTTCTTTACA+TGG 0.585974 8.4:+1943929 MS.gene042263:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8.4:+1942808 MS.gene042263:CDS
AACATTTGCATTATTGACAG+TGG 0.591551 8.4:-1944077 None:intergenic
GGGTTCAGTAGGTGGATATG+GGG 0.592030 8.4:+1943748 MS.gene042263:CDS
CTAGTTCATGATGGTTGTGG+AGG 0.595087 8.4:+1940789 None:intergenic
AGAAGAAGCAAGATAATCCA+AGG 0.597269 8.4:-1944449 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8.4:+1942780 MS.gene042263:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8.4:+1943014 MS.gene042263:CDS
ATGTTATGAGACTGAAGTGG+AGG 0.606395 8.4:-1943872 None:intergenic
GAATAGAACTTCTCAACTTG+AGG 0.607973 8.4:-1944538 None:intergenic
GCTAACAAGAATAAAACGCA+TGG 0.616521 8.4:+1943153 MS.gene042263:CDS
GGTAAATGATGATATAGAGA+AGG 0.617327 8.4:+1942957 MS.gene042263:CDS
AATGTCAAGATGGCTTACTT+GGG 0.618703 8.4:+1944365 MS.gene042263:CDS
TACCAGCAAAAGGGTTCAGT+AGG 0.620378 8.4:+1943737 MS.gene042263:CDS
TAGAGGAACCACTTCTGACA+GGG 0.620617 8.4:-1944204 None:intergenic
ATCCAAGGTATTGTTGAAGG+AGG 0.626426 8.4:-1944434 None:intergenic
GTCACATAAACTCACTGTCA+AGG 0.629956 8.4:-1943314 None:intergenic
TCAAGGTATCATTAGTGATG+GGG 0.639460 8.4:+1943098 MS.gene042263:CDS
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8.4:-1942847 None:intergenic
TCTGATGTCTTGGAAGCCAG+AGG 0.643398 8.4:-1944053 None:intergenic
CTTAAATTAATGTTATCAGG+CGG 0.647690 8.4:-1943998 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8.4:+1942784 MS.gene042263:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8.4:-1942843 None:intergenic
GTGATGAAGAGTGTGATACA+TGG 0.658673 8.4:+1942979 MS.gene042263:CDS
TGTTATGAGACTGAAGTGGA+GGG 0.664363 8.4:-1943871 None:intergenic
GGAATGTTATGAGACTGAAG+TGG 0.672395 8.4:-1943875 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+1943464 MS.gene042263:CDS
TCATGATCCCAAACTAAACG+AGG 0.706682 8.4:-1943252 None:intergenic
TTTAGGAGAAGGTTTCCCGG+CGG 0.790759 8.4:+1940834 MS.gene042263:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAAATCTAAAACAAAA+GGG + chr8.4:1942413-1942432 MS.gene042263:intron 10.0%
!!! TTAAAAAAATCTAAAACAAA+AGG + chr8.4:1942412-1942431 MS.gene042263:intron 10.0%
!!! TTTTTATGATAGTAAATAAA+TGG - chr8.4:1942181-1942200 None:intergenic 10.0%
!!! TTTTTTTCTTTTTTTTTTTG+AGG - chr8.4:1941970-1941989 None:intergenic 10.0%
!! AAATAAATGGAAATTATCTT+CGG - chr8.4:1942168-1942187 None:intergenic 15.0%
!! CAATTTACTAAAAAAAACTA+AGG - chr8.4:1941098-1941117 None:intergenic 15.0%
!!! CTTAGTTTTTTTTAGTAAAT+TGG + chr8.4:1941096-1941115 MS.gene042263:intron 15.0%
!! AAATATCCGAATATAAGAAA+TGG - chr8.4:1942912-1942931 None:intergenic 20.0%
!! AATGATTATTACTGCTTTAT+TGG + chr8.4:1942479-1942498 MS.gene042263:intron 20.0%
!! ATAAGAAATGGATCTTAATT+AGG - chr8.4:1942900-1942919 None:intergenic 20.0%
!! GAAGAGACATTTAAATAAAA+AGG - chr8.4:1942645-1942664 None:intergenic 20.0%
!! TAAGATCCATTTCTTATATT+CGG + chr8.4:1942903-1942922 MS.gene042263:intron 20.0%
!! TGATGAATGATTCTTAAATA+TGG + chr8.4:1943366-1943385 MS.gene042263:intron 20.0%
!! TGATTAATGAACTATTAGTT+TGG - chr8.4:1944510-1944529 None:intergenic 20.0%
!!! AAACCTTTTTTTTTTCTTTG+AGG + chr8.4:1941057-1941076 MS.gene042263:intron 20.0%
!!! AGATGTGTTACAATTTTTTA+GGG - chr8.4:1941610-1941629 None:intergenic 20.0%
!!! ATGATTATTACTGCTTTATT+GGG + chr8.4:1942480-1942499 MS.gene042263:intron 20.0%
!!! ATTCTTGTTAGCAATATTTT+CGG - chr8.4:1943145-1943164 None:intergenic 20.0%
!!! GATTAATGAACTATTAGTTT+GGG - chr8.4:1944509-1944528 None:intergenic 20.0%
!!! TTTGAGATTTTTTGACAAAA+GGG - chr8.4:1943534-1943553 None:intergenic 20.0%
!!! TTTTTAGTAAATTGGTGATT+AGG + chr8.4:1941104-1941123 MS.gene042263:intron 20.0%
! AAAAAAATCACTAACATGTC+AGG - chr8.4:1942506-1942525 None:intergenic 25.0%
! AACTACAACCTATTTATCAT+AGG + chr8.4:1941819-1941838 MS.gene042263:intron 25.0%
! AAGTTACTCAATAGTTATAG+TGG - chr8.4:1941347-1941366 None:intergenic 25.0%
! ACACCAATAAAACTTATTTG+TGG + chr8.4:1941170-1941189 MS.gene042263:intron 25.0%
! AGTGTATATTTATGCTTCAT+AGG + chr8.4:1941909-1941928 MS.gene042263:intron 25.0%
! AGTTGAAGAAACTACATATT+AGG - chr8.4:1943403-1943422 None:intergenic 25.0%
! ATTGAATGAACGAAAAAGAA+AGG - chr8.4:1940949-1940968 None:intergenic 25.0%
! CTTAAATTAATGTTATCAGG+CGG - chr8.4:1944001-1944020 None:intergenic 25.0%
! GATACAGAATTGATATCAAA+TGG + chr8.4:1943675-1943694 MS.gene042263:CDS 25.0%
! GGACTTAAATTAATGTTATC+AGG - chr8.4:1944004-1944023 None:intergenic 25.0%
! GTTCTAGTATGATTTAAACA+TGG - chr8.4:1941796-1941815 None:intergenic 25.0%
! TTACATAGCCTATGATAAAT+AGG - chr8.4:1941830-1941849 None:intergenic 25.0%
!! AAAAATTTGTTTGTTCCGTT+CGG + chr8.4:1941279-1941298 MS.gene042263:intron 25.0%
!! AATTTTCATATGAGAATTGC+TGG + chr8.4:1942595-1942614 MS.gene042263:intron 25.0%
!! ATATAATTTGTACCTGCAAA+TGG - chr8.4:1943503-1943522 None:intergenic 25.0%
!! ATCAAGTCAAGTATTTTCTT+AGG - chr8.4:1943444-1943463 None:intergenic 25.0%
!! GGTCTTTTATATAATAATGC+TGG + chr8.4:1942616-1942635 MS.gene042263:intron 25.0%
!! TTTTTCCTTCTTTTTCTGAA+TGG + chr8.4:1942525-1942544 MS.gene042263:intron 25.0%
!!! CAAAACAGATTTTAAGAGAA+AGG - chr8.4:1942873-1942892 None:intergenic 25.0%
!!! GAGATGTGTTACAATTTTTT+AGG - chr8.4:1941611-1941630 None:intergenic 25.0%
!!! GTTTGAGATTTTTTGACAAA+AGG - chr8.4:1943535-1943554 None:intergenic 25.0%
!!! TTGAATAACTTTTCGATTTG+AGG + chr8.4:1942278-1942297 MS.gene042263:intron 25.0%
AACATTTGCATTATTGACAG+TGG - chr8.4:1944080-1944099 None:intergenic 30.0%
ACGCCTCAAAGAAAAAAAAA+AGG - chr8.4:1941063-1941082 None:intergenic 30.0%
ACTCAATAGTTATAGTGGTA+CGG - chr8.4:1941342-1941361 None:intergenic 30.0%
ACTGCATTTGAATCATCAAT+TGG - chr8.4:1944025-1944044 None:intergenic 30.0%
ATAGGCTATGTAAATGTATC+CGG + chr8.4:1941837-1941856 MS.gene042263:intron 30.0%
ATCCTAATAGTGTTTCTCTA+AGG + chr8.4:1941428-1941447 MS.gene042263:intron 30.0%
ATGTAGAGAGTAGAAAAACA+AGG - chr8.4:1941540-1941559 None:intergenic 30.0%
CTTTCACTTTCAGAAAACAT+TGG - chr8.4:1943914-1943933 None:intergenic 30.0%
CTTTGGCAAAAGAATGAATT+AGG - chr8.4:1941376-1941395 None:intergenic 30.0%
GATAAATATTCTGCTGAAAC+TGG - chr8.4:1943979-1943998 None:intergenic 30.0%
GATTATCTTGCTTCTTCTAA+TGG + chr8.4:1944454-1944473 MS.gene042263:CDS 30.0%
GGTAAATGATGATATAGAGA+AGG + chr8.4:1942957-1942976 MS.gene042263:CDS 30.0%
TAAACCTTGTATCAGATGAA+AGG - chr8.4:1941569-1941588 None:intergenic 30.0%
TAGAAACCACAACAAAACTA+GGG - chr8.4:1941514-1941533 None:intergenic 30.0%
TAGAAAGAAGATTGAAAGCA+AGG + chr8.4:1943068-1943087 MS.gene042263:CDS 30.0%
TATCAAATGGCTATCTAAAC+AGG + chr8.4:1943688-1943707 MS.gene042263:CDS 30.0%
TCAAAACCACACCTAAAATT+GGG + chr8.4:1941723-1941742 MS.gene042263:intron 30.0%
TGAATTGTTGAGAGAAAAGA+AGG + chr8.4:1942735-1942754 MS.gene042263:CDS 30.0%
TGATTTAAACATGGTGTTTC+TGG - chr8.4:1941787-1941806 None:intergenic 30.0%
TGCAATCGAAAATGAAAATG+AGG - chr8.4:1943653-1943672 None:intergenic 30.0%
TGCTAACATCACTAATTACA+AGG - chr8.4:1942771-1942790 None:intergenic 30.0%
TGTAGAGAGTAGAAAAACAA+GGG - chr8.4:1941539-1941558 None:intergenic 30.0%
TGTGTGATTTATAGTGTTGT+CGG + chr8.4:1942936-1942955 MS.gene042263:intron 30.0%
TTAGAAACCACAACAAAACT+AGG - chr8.4:1941515-1941534 None:intergenic 30.0%
TTCTTCAACTATTATTGCAG+AGG + chr8.4:1943413-1943432 MS.gene042263:intron 30.0%
TTGTTAAAATCCTCACTCAA+TGG - chr8.4:1944250-1944269 None:intergenic 30.0%
TTTCAACCGTATTTGCAAAA+AGG - chr8.4:1943583-1943602 None:intergenic 30.0%
! AAAAAGAAAGGTACAAAGTG+AGG + chr8.4:1943179-1943198 MS.gene042263:CDS 30.0%
! AATCAAGGTATCATTAGTGA+TGG + chr8.4:1943096-1943115 MS.gene042263:CDS 30.0%
! ATCAAGGTATCATTAGTGAT+GGG + chr8.4:1943097-1943116 MS.gene042263:CDS 30.0%
! TAAATCACTAGCTTGATTCT+TGG + chr8.4:1942667-1942686 MS.gene042263:intron 30.0%
! TTGACTTGATGAATGTTGAA+GGG + chr8.4:1943454-1943473 MS.gene042263:CDS 30.0%
! TTTTGATGTTGTGAGTAACA+TGG - chr8.4:1944406-1944425 None:intergenic 30.0%
! TTTTGCCATTCAGAAAAAGA+AGG - chr8.4:1942533-1942552 None:intergenic 30.0%
!! ATAATCCAAGGTATTGTTGA+AGG - chr8.4:1944440-1944459 None:intergenic 30.0%
!! GCTCCACAAATAAGTTTTAT+TGG - chr8.4:1941176-1941195 None:intergenic 30.0%
!! TCAAGTTGAGAAGTTCTATT+CGG + chr8.4:1944540-1944559 MS.gene042263:CDS 30.0%
!! TTGGAAGTAGCTTTGATATA+AGG + chr8.4:1941034-1941053 MS.gene042263:intron 30.0%
AAAAGAAGCCTCGTTTAGTT+TGG + chr8.4:1943244-1943263 MS.gene042263:CDS 35.0%
AACGCATGGACAAAAAAGAA+AGG + chr8.4:1943167-1943186 MS.gene042263:CDS 35.0%
AATGTCAAGATGGCTTACTT+GGG + chr8.4:1944365-1944384 MS.gene042263:CDS 35.0%
ACTATTAGGATTGAGTCTCT+TGG - chr8.4:1941419-1941438 None:intergenic 35.0%
AGAAGAAGCAAGATAATCCA+AGG - chr8.4:1944452-1944471 None:intergenic 35.0%
AGAATTGGAAGCAAAGTTAG+AGG - chr8.4:1944224-1944243 None:intergenic 35.0%
ATAAATCACTCTCCTGTTGA+AGG + chr8.4:1944154-1944173 MS.gene042263:CDS 35.0%
CAAGGAATCATGGAATCAAA+TGG + chr8.4:1944565-1944584 MS.gene042263:CDS 35.0%
CACCTTAGAGAAACACTATT+AGG - chr8.4:1941433-1941452 None:intergenic 35.0%
CACTAATACATATCAGACTC+CGG - chr8.4:1941859-1941878 None:intergenic 35.0%
CTCAAAACCACACCTAAAAT+TGG + chr8.4:1941722-1941741 MS.gene042263:intron 35.0%
CTTCTCACATGCAACTATAA+TGG + chr8.4:1940916-1940935 MS.gene042263:CDS 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.4:1943081-1943100 MS.gene042263:CDS 35.0%
GAATAGAACTTCTCAACTTG+AGG - chr8.4:1944541-1944560 None:intergenic 35.0%
GAGCAATTGCTTAATGTAGA+TGG + chr8.4:1944601-1944620 MS.gene042263:CDS 35.0%
GCAACGGTGTAAACATATAT+TGG + chr8.4:1941015-1941034 MS.gene042263:intron 35.0%
GCTAACAAGAATAAAACGCA+TGG + chr8.4:1943153-1943172 MS.gene042263:CDS 35.0%
GTCGAAGAAAAATGAGAACT+GGG - chr8.4:1944310-1944329 None:intergenic 35.0%
GTTTCTGCAGTTAATCTTGT+CGG + chr8.4:1943291-1943310 MS.gene042263:CDS 35.0%
TACTACAACTACGAAGTCAT+TGG + chr8.4:1942705-1942724 MS.gene042263:CDS 35.0%
TCTCTTTGCAACAAACACTA+GGG - chr8.4:1941479-1941498 None:intergenic 35.0%
TGTCGAAGAAAAATGAGAAC+TGG - chr8.4:1944311-1944330 None:intergenic 35.0%
TTAAGCTCTTCCATTCGAAT+CGG - chr8.4:1943027-1943046 None:intergenic 35.0%
TTCTCTTTGCAACAAACACT+AGG - chr8.4:1941480-1941499 None:intergenic 35.0%
! AAACATTGGTGTCTCGAAAT+TGG - chr8.4:1943900-1943919 None:intergenic 35.0%
! AAAGAAGCCTCGTTTAGTTT+GGG + chr8.4:1943245-1943264 MS.gene042263:CDS 35.0%
! AACATTGGTGTCTCGAAATT+GGG - chr8.4:1943899-1943918 None:intergenic 35.0%
! ATCATTAGTGATGGGGTATA+TGG + chr8.4:1943105-1943124 MS.gene042263:CDS 35.0%
! CATCCAATGACATGTTTTGT+CGG + chr8.4:1943795-1943814 MS.gene042263:CDS 35.0%
! CTTGACTTGATGAATGTTGA+AGG + chr8.4:1943453-1943472 MS.gene042263:CDS 35.0%
! GAAAGTTCGAGTTCTTTACA+TGG + chr8.4:1943929-1943948 MS.gene042263:CDS 35.0%
! GTTAGCAATATTTTCGGATG+AGG - chr8.4:1943139-1943158 None:intergenic 35.0%
! TCAAGGTATCATTAGTGATG+GGG + chr8.4:1943098-1943117 MS.gene042263:CDS 35.0%
! TCACCTCCTTTTTGCAAATA+CGG + chr8.4:1943574-1943593 MS.gene042263:intron 35.0%
! TTTGAGGTAGCTTAGACATT+CGG - chr8.4:1941954-1941973 None:intergenic 35.0%
!!! GGTGATTAGGTTAGTTTTCA+CGG + chr8.4:1941117-1941136 MS.gene042263:intron 35.0%
!!! GTTAGTTTTCACGGTTTGAA+CGG + chr8.4:1941126-1941145 MS.gene042263:intron 35.0%
!!! TACAAACCCTAGTTTTGTTG+TGG + chr8.4:1941505-1941524 MS.gene042263:intron 35.0%
AAATGTATCCAGCCATTTGC+AGG + chr8.4:1943488-1943507 MS.gene042263:CDS 40.0%
AATGCAGTTACAGATTCCTC+TGG + chr8.4:1944037-1944056 MS.gene042263:CDS 40.0%
AATTTGTACCTGCAAATGGC+TGG - chr8.4:1943499-1943518 None:intergenic 40.0%
ACATGGCATTCAAACGTCAA+TGG + chr8.4:1943946-1943965 MS.gene042263:CDS 40.0%
ACTATTCTCCTCTCATGCAA+CGG + chr8.4:1940999-1941018 MS.gene042263:intron 40.0%
ATCAAGCAACCAAAACAACG+TGG + chr8.4:1941202-1941221 MS.gene042263:intron 40.0%
ATGGTTGTGGAGGATAGTTT+CGG + chr8.4:1940799-1940818 MS.gene042263:CDS 40.0%
ATGTCATTGGATGCATATGC+TGG - chr8.4:1943788-1943807 None:intergenic 40.0%
ATGTTATGAGACTGAAGTGG+AGG - chr8.4:1943875-1943894 None:intergenic 40.0%
CAACCGTATTTGCAAAAAGG+AGG - chr8.4:1943580-1943599 None:intergenic 40.0%
CAACTGATATGTGTGTGAGT+AGG - chr8.4:1942008-1942027 None:intergenic 40.0%
CAATGTCAAGATGGCTTACT+TGG + chr8.4:1944364-1944383 MS.gene042263:CDS 40.0%
CAATTCTGTTCCATTGAGTG+AGG + chr8.4:1944237-1944256 MS.gene042263:CDS 40.0%
CATTCCTTCCAAGTTCAAAC+TGG - chr8.4:1943834-1943853 None:intergenic 40.0%
CTATTCGGATCAAGGAATCA+TGG + chr8.4:1944555-1944574 MS.gene042263:CDS 40.0%
CTCACCTTTCATCTGATACA+AGG + chr8.4:1941562-1941581 MS.gene042263:intron 40.0%
CTCACTCAATGGAACAGAAT+TGG - chr8.4:1944239-1944258 None:intergenic 40.0%
GAAACGATGCAATGTCAAGA+TGG + chr8.4:1944355-1944374 MS.gene042263:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:1943464-1943483 MS.gene042263:CDS 40.0%
GAGAAGTTCTATTCGGATCA+AGG + chr8.4:1944547-1944566 MS.gene042263:CDS 40.0%
GGAATGTTATGAGACTGAAG+TGG - chr8.4:1943878-1943897 None:intergenic 40.0%
GTCACATAAACTCACTGTCA+AGG - chr8.4:1943317-1943336 None:intergenic 40.0%
TAATGGAGACACTAGTTCCA+TGG + chr8.4:1944471-1944490 MS.gene042263:CDS 40.0%
TCAAGCAACCAAAACAACGT+GGG + chr8.4:1941203-1941222 MS.gene042263:intron 40.0%
TCATGATCCCAAACTAAACG+AGG - chr8.4:1943255-1943274 None:intergenic 40.0%
TCCAACTTATCTCAAAGTGC+TGG + chr8.4:1940885-1940904 MS.gene042263:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:1943463-1943482 MS.gene042263:CDS 40.0%
TGAGCTTACTGATGAAAGGT+AGG - chr8.4:1942846-1942865 None:intergenic 40.0%
TGGCTATCTAAACAGGATAG+AGG + chr8.4:1943695-1943714 MS.gene042263:CDS 40.0%
TGTGTGAGCTTACTGATGAA+AGG - chr8.4:1942850-1942869 None:intergenic 40.0%
TGTTATGAGACTGAAGTGGA+GGG - chr8.4:1943874-1943893 None:intergenic 40.0%
TTCCTCCTTCAACAATACCT+TGG + chr8.4:1944432-1944451 MS.gene042263:CDS 40.0%
TTGAGGTAGCTTAGACATTC+GGG - chr8.4:1941953-1941972 None:intergenic 40.0%
TTGCAACAAACACTAGGGTT+GGG - chr8.4:1941474-1941493 None:intergenic 40.0%
TTGGGCTAAACTTTGTACCA+TGG - chr8.4:1944491-1944510 None:intergenic 40.0%
TTTGCAACAAACACTAGGGT+TGG - chr8.4:1941475-1941494 None:intergenic 40.0%
! AAGAAAGGTACAAAGTGAGG+AGG + chr8.4:1943182-1943201 MS.gene042263:CDS 40.0%
! ACTAAACGAGGCTTCTTTTG+AGG - chr8.4:1943243-1943262 None:intergenic 40.0%
! AGACTCCGATTTTCTCACTT+CGG + chr8.4:1941674-1941693 MS.gene042263:intron 40.0%
! GTGATGAAGAGTGTGATACA+TGG + chr8.4:1942979-1942998 MS.gene042263:CDS 40.0%
! TCTTTTGCCAAGACTGTTGT+GGG + chr8.4:1942061-1942080 MS.gene042263:intron 40.0%
! TGGAAGAGCTTAAGAGCATA+TGG + chr8.4:1943034-1943053 MS.gene042263:CDS 40.0%
! TGTTGTGGGTGTGTTTGTTT+TGG + chr8.4:1942075-1942094 MS.gene042263:intron 40.0%
! TTCTTTTGCCAAGACTGTTG+TGG + chr8.4:1942060-1942079 MS.gene042263:intron 40.0%
!! ATCCAAGGTATTGTTGAAGG+AGG - chr8.4:1944437-1944456 None:intergenic 40.0%
!! TCCAGCACTTTGAGATAAGT+TGG - chr8.4:1940889-1940908 None:intergenic 40.0%
!! TCTAGTGAAACCGATTCGAA+TGG + chr8.4:1943014-1943033 MS.gene042263:CDS 40.0%
!!! GTTGACGATGTTTTAGGAGA+AGG + chr8.4:1940823-1940842 MS.gene042263:CDS 40.0%
!!! TGTTTTAGGAGAAGGTTTCC+CGG + chr8.4:1940831-1940850 MS.gene042263:CDS 40.0%
!!! TTCGGTGTTGACGATGTTTT+AGG + chr8.4:1940817-1940836 MS.gene042263:CDS 40.0%
AAACACACCCACAACAGTCT+TGG - chr8.4:1942071-1942090 None:intergenic 45.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8.4:1943746-1943765 MS.gene042263:CDS 45.0%
AGGATTGAGTCTCTTGGTCA+CGG - chr8.4:1941413-1941432 None:intergenic 45.0%
AGGGTTCAGTAGGTGGATAT+GGG + chr8.4:1943747-1943766 MS.gene042263:CDS 45.0%
CACACCATCCAGTTTGAACT+TGG + chr8.4:1943823-1943842 MS.gene042263:CDS 45.0%
CAGCCGACAAAACATGTCAT+TGG - chr8.4:1943801-1943820 None:intergenic 45.0%
CATTGCATCGTTTCCTCCAA+AGG - chr8.4:1944349-1944368 None:intergenic 45.0%
CCAATACTGACCTACTTTGG+AGG + chr8.4:1941303-1941322 MS.gene042263:intron 45.0%
CCACTTCTGACAGGGTTAAA+AGG - chr8.4:1944199-1944218 None:intergenic 45.0%
CCATCCAGTTTGAACTTGGA+AGG + chr8.4:1943827-1943846 MS.gene042263:CDS 45.0%
CCTCCAAAGTAGGTCAGTAT+TGG - chr8.4:1941306-1941325 None:intergenic 45.0%
CCTTCCAAGTTCAAACTGGA+TGG - chr8.4:1943830-1943849 None:intergenic 45.0%
CGGTTTGAACGGTTGTGAAT+CGG + chr8.4:1941137-1941156 MS.gene042263:intron 45.0%
CTTGGTCACGGAAAGAGATT+CGG - chr8.4:1941401-1941420 None:intergenic 45.0%
GAAGTGAGAAAATCGGAGTC+TGG - chr8.4:1941675-1941694 None:intergenic 45.0%
GGTCCAATACTGACCTACTT+TGG + chr8.4:1941300-1941319 MS.gene042263:intron 45.0%
TACCAGCAAAAGGGTTCAGT+AGG + chr8.4:1943737-1943756 MS.gene042263:CDS 45.0%
TAGAGGAACCACTTCTGACA+GGG - chr8.4:1944207-1944226 None:intergenic 45.0%
TCAGCAAGTCGGACTTGAAA+TGG + chr8.4:1942819-1942838 MS.gene042263:CDS 45.0%
TGATGTTAGCATGCCAGACA+TGG + chr8.4:1942780-1942799 MS.gene042263:CDS 45.0%
TGTTTACACCGTTGCATGAG+AGG - chr8.4:1941010-1941029 None:intergenic 45.0%
TTAGAGGAACCACTTCTGAC+AGG - chr8.4:1944208-1944227 None:intergenic 45.0%
TTCAAGCTCCTTCAGCAAGT+CGG + chr8.4:1942808-1942827 MS.gene042263:CDS 45.0%
TTCACAGCAGAACCTTCAAC+AGG - chr8.4:1944169-1944188 None:intergenic 45.0%
TTCAGAATAGTCGTGTGCCT+TGG + chr8.4:1944629-1944648 MS.gene042263:CDS 45.0%
TTGGATCCCTACCAGCAAAA+GGG + chr8.4:1943728-1943747 MS.gene042263:CDS 45.0%
TTGGTCACGGAAAGAGATTC+GGG - chr8.4:1941400-1941419 None:intergenic 45.0%
! ACTGAACCCTTTTGCTGGTA+GGG - chr8.4:1943737-1943756 None:intergenic 45.0%
! AGACATCGTTGCTGTTCCTT+TGG + chr8.4:1944330-1944349 MS.gene042263:CDS 45.0%
! AGCAATCGAGGCCCAATTTT+AGG - chr8.4:1941737-1941756 None:intergenic 45.0%
! CCTTTTAACCCTGTCAGAAG+TGG + chr8.4:1944196-1944215 MS.gene042263:CDS 45.0%
! GACTCCGATTTTCTCACTTC+GGG + chr8.4:1941675-1941694 MS.gene042263:intron 45.0%
! GTGTGAGTAGGTAGCTTTAC+TGG - chr8.4:1941996-1942015 None:intergenic 45.0%
! TACTGAACCCTTTTGCTGGT+AGG - chr8.4:1943738-1943757 None:intergenic 45.0%
! TAGGTCAGTATTGGACCGAA+CGG - chr8.4:1941297-1941316 None:intergenic 45.0%
! TCTGGAACTGCCAAAATCGA+CGG - chr8.4:1941769-1941788 None:intergenic 45.0%
!! TTTCAAGTCCGACTTGCTGA+AGG - chr8.4:1942819-1942838 None:intergenic 45.0%
!!! AAAATATTTTGTTAAAAAAA+TGG - chr8.4:1942316-1942335 None:intergenic 5.0%
!!! AAATTATATAAGTATTTTTT+TGG + chr8.4:1942363-1942382 MS.gene042263:intron 5.0%
AGCAACCAAAACAACGTGGG+TGG + chr8.4:1941206-1941225 MS.gene042263:intron 50.0%
AGCTTGAAGCCATCCATGTC+TGG - chr8.4:1942796-1942815 None:intergenic 50.0%
CAGCAAAAGGGTTCAGTAGG+TGG + chr8.4:1943740-1943759 MS.gene042263:CDS 50.0%
CGGAAAGAGATTCGGGACTT+TGG - chr8.4:1941393-1941412 None:intergenic 50.0%
GGATAGAGGAGATGATGCGT+TGG + chr8.4:1943709-1943728 MS.gene042263:CDS 50.0%
GGGTTCAGTAGGTGGATATG+GGG + chr8.4:1943748-1943767 MS.gene042263:CDS 50.0%
GTTAGCATGCCAGACATGGA+TGG + chr8.4:1942784-1942803 MS.gene042263:CDS 50.0%
GTTGGATCCCTACCAGCAAA+AGG + chr8.4:1943727-1943746 MS.gene042263:CDS 50.0%
TCTGATGTCTTGGAAGCCAG+AGG - chr8.4:1944056-1944075 None:intergenic 50.0%
TGCGCCCGAAGTGAGAAAAT+CGG - chr8.4:1941682-1941701 None:intergenic 50.0%
TTTAGGAGAAGGTTTCCCGG+CGG + chr8.4:1940834-1940853 MS.gene042263:CDS 50.0%
! CACCTACTGAACCCTTTTGC+TGG - chr8.4:1943742-1943761 None:intergenic 50.0%
! CATCGTTGCTGTTCCTTTGG+AGG + chr8.4:1944333-1944352 MS.gene042263:CDS 50.0%
!! TCGAGGCCCAATTTTAGGTG+TGG - chr8.4:1941732-1941751 None:intergenic 50.0%
!! TGACAGTGGCTCTGATGTCT+TGG - chr8.4:1944066-1944085 None:intergenic 50.0%
!!! TCCAAATCGGCCGTCGATTT+TGG + chr8.4:1941756-1941775 MS.gene042263:intron 50.0%
GCCAAAATCGACGGCCGATT+TGG - chr8.4:1941760-1941779 None:intergenic 55.0%
GCTGCTCTCTCCTCCAAAGT+AGG - chr8.4:1941316-1941335 None:intergenic 55.0%
GGGCCTCGATTGCTCCAAAT+CGG + chr8.4:1941743-1941762 MS.gene042263:intron 55.0%
TTAGGAGAAGGTTTCCCGGC+GGG + chr8.4:1940835-1940854 MS.gene042263:CDS 55.0%
! AGCTGCCACCCACGTTGTTT+TGG - chr8.4:1941214-1941233 None:intergenic 55.0%
!! CGGCCGATTTGGAGCAATCG+AGG - chr8.4:1941749-1941768 None:intergenic 60.0%
CCAGAACACGCATACCCGCC+GGG - chr8.4:1940852-1940871 None:intergenic 65.0%
GCCAGAACACGCATACCCGC+CGG - chr8.4:1940853-1940872 None:intergenic 65.0%
CCCGGCGGGTATGCGTGTTC+TGG + chr8.4:1940849-1940868 MS.gene042263:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1940796 1944663 1940796 ID=MS.gene042263
chr8.4 mRNA 1940796 1944663 1940796 ID=MS.gene042263.t1;Parent=MS.gene042263
chr8.4 exon 1940796 1940937 1940796 ID=MS.gene042263.t1.exon1;Parent=MS.gene042263.t1
chr8.4 CDS 1940796 1940937 1940796 ID=cds.MS.gene042263.t1;Parent=MS.gene042263.t1
chr8.4 exon 1942704 1942856 1942704 ID=MS.gene042263.t1.exon2;Parent=MS.gene042263.t1
chr8.4 CDS 1942704 1942856 1942704 ID=cds.MS.gene042263.t1;Parent=MS.gene042263.t1
chr8.4 exon 1942950 1943321 1942950 ID=MS.gene042263.t1.exon3;Parent=MS.gene042263.t1
chr8.4 CDS 1942950 1943321 1942950 ID=cds.MS.gene042263.t1;Parent=MS.gene042263.t1
chr8.4 exon 1943433 1943509 1943433 ID=MS.gene042263.t1.exon4;Parent=MS.gene042263.t1
chr8.4 CDS 1943433 1943509 1943433 ID=cds.MS.gene042263.t1;Parent=MS.gene042263.t1
chr8.4 exon 1943674 1944663 1943674 ID=MS.gene042263.t1.exon5;Parent=MS.gene042263.t1
chr8.4 CDS 1943674 1944663 1943674 ID=cds.MS.gene042263.t1;Parent=MS.gene042263.t1
Gene Sequence

>MS.gene042263

ATGATGGTTGTGGAGGATAGTTTCGGTGTTGACGATGTTTTAGGAGAAGGTTTCCCGGCGGGTATGCGTGTTCTGGCAGTTGATGACGATCCAACTTATCTCAAAGTGCTGGAGAAGCAACTTCTCACATGCAACTATAATGGTTCGTTGCCTTTCTTTTTCGTTCATTCAATATGATTACGCATTGATTATTCTTTATGTTTACTATTCTCCTCTCATGCAACGGTGTAAACATATATTGGAAGTAGCTTTGATATAAGGAAACCTTTTTTTTTTCTTTGAGGCGTTGATATAGTAAACCTTAGTTTTTTTTAGTAAATTGGTGATTAGGTTAGTTTTCACGGTTTGAACGGTTGTGAATCGGTTAAAAAATCACACCAATAAAACTTATTTGTGGAGCAAATGAATCAAGCAACCAAAACAACGTGGGTGGCAGCTCTAAACACAATCAAAAGATAGAACAACAAAAGAGAAAGAACACACAAAAATTTGTTTGTTCCGTTCGGTCCAATACTGACCTACTTTGGAGGAGAGAGCAGCTCTCCGTACCACTATAACTATTGAGTAACTTTACAAACCTAATTCATTCTTTTGCCAAAGTCCCGAATCTCTTTCCGTGACCAAGAGACTCAATCCTAATAGTGTTTCTCTAAGGTGAATCAATGATGACTCAATCCCAACCCTAGTGTTTGTTGCAAAGAGAAAACTCTACAAACCCTAGTTTTGTTGTGGTTTCTAAACCCTTGTTTTTCTACTCTCTACATCTCTCACCTTTCATCTGATACAAGGTTTATATTTATAGTAAAAGCATCCCTAAAAAATTGTAACACATCTCATGCTGAAAATACGTTTCTGTTTTTTCTGCATCATTAAAAACCAGACTCCGATTTTCTCACTTCGGGCGCAGATTTTTACGAGACAGAAGTCTCAAAACCACACCTAAAATTGGGCCTCGATTGCTCCAAATCGGCCGTCGATTTTGGCAGTTCCAGAAACACCATGTTTAAATCATACTAGAACATGAACTACAACCTATTTATCATAGGCTATGTAAATGTATCCGGAGTCTGATATGTATTAGTGTCTGATACAATTTGACGCATGTCATGTGTTAGTGTATATTTATGCTTCATAGGTTATAAGTCTTCTAGTGTCCCGAATGTCTAAGCTACCTCAAAAAAAAAAAGAAAAAAACTACCAGTAAAGCTACCTACTCACACACATATCAGTTGACAATTTTCTTGATTTTAAATACATAAATTCATTCTTTTGCCAAGACTGTTGTGGGTGTGTTTGTTTTGGCTTAAAATAAAATTATTTTGTTTTTATAAAATATAAATATTATTTTTGAGATCTATAAAAAAAAAAGCCGAAGATAATTTCCATTTATTTACTATCATAAAAATAATTTTTTTAAGATTTAAAACACTTACACTTGAGTTTCTTGTTTTTTAAAAGAAAAATAGATTTTGAAAAAGCTATTTGAATAACTTTTCGATTTGAGGCTAAAAATTACTCCATTTTTTTAACAAAATATTTTTAAAAAAAATCTGAAACAAACACTATAAAATTATATAAGTATTTTTTTGGTTTAAAAAATTGATTTTTTGTTTCTTTTAAAAAAATCTAAAACAAAAGGGTCTGTGTTTTCTCTACTTCTAAAGTTGAAATTTTAAACTTTTTAATGATTATTACTGCTTTATTGGGCCTGACATGTTAGTGATTTTTTTTTTTCCTTCTTTTTCTGAATGGCAAAAGAAAATTTTATAAAATAAGATGATACTAATCTTATTTAATAAAATTTTCATATGAGAATTGCTGGTCTTTTATATAATAATGCTGGAAGCCTTTTTATTTAAATGTCTCTTCACTAAATCACTAGCTTGATTCTTGGTTGCATTGTTGCAGTTACTACAACTACGAAGTCATTGGAAGCGCTTGAATTGTTGAGAGAAAAGAAGGATATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCAGTAAGCTCACACACCTTTCTCTTAAAATCTGTTTTGTTTACCTAATTAAGATCCATTTCTTATATTCGGATATTTTGTGTGTGTGATTTATAGTGTTGTCGGTAAATGATGATATAGAGAAGGTGATGAAGAGTGTGATACATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGCCTTGACAGTGAGTTTATGTGACAATGAAATTATTTTTTTACTTTGCTAATTTGATGAATGATTCTTAAATATGGATATTATTGTTCCTAATATGTAGTTTCTTCAACTATTATTGCAGAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAGGTACAAATTATATATAACTTTCCCTTTTGTCAAAAAATCTCAAACTAGTTTATCATTAAAATATTCACCTCCTTTTTGCAAATACGGTTGAAACTTCAACAAGCAAGTTTATGAAACGTAAGTATAAAAACTGTGTGTAACCTCATTTTCATTTTCGATTGCAGAGATACAGAATTGATATCAAATGGCTATCTAAACAGGATAGAGGAGATGATGCGTTGGATCCCTACCAGCAAAAGGGTTCAGTAGGTGGATATGGGGATTTTTGCACACTGCCAGCATATGCATCCAATGACATGTTTTGTCGGCTGAACACACCATCCAGTTTGAACTTGGAAGGAATGAGTTCTTCTGCACTAGTCCCTCCACTTCAGTCTCATAACATTCCCAATTTCGAGACACCAATGTTTTCTGAAAGTGAAAGTTCGAGTTCTTTACATGGCATTCAAACGTCAATGGAAATTAACCAGTTTCAGCAGAATATTTATCCGCCTGATAACATTAATTTAAGTCCAATTGATGATTCAAATGCAGTTACAGATTCCTCTGGCTTCCAAGACATCAGAGCCACTGTCAATAATGCAAATGTTTCTCTTTCTTGCATCGCAAGCAATCACATACAAACACATAAATCAGAAGCATTTATAAATCACTCTCCTGTTGAAGGTTCTGCTGTGAAAACACAACCTTTTAACCCTGTCAGAAGTGGTTCCTCTAACTTTGCTTCCAATTCTGTTCCATTGAGTGAGGATTTTAACAATGATCAAATGTTGCACAATAACTACAAGTTTACTTCTCCCAGTTCTCATTTTTCTTCGACAGACATCGTTGCTGTTCCTTTGGAGGAAACGATGCAATGTCAAGATGGCTTACTTGGGAATGTTATAAATGCTCCATGTTACTCACAACATCAAAATGCGAGTTCCTCCTTCAACAATACCTTGGATTATCTTGCTTCTTCTAATGGAGACACTAGTTCCATGGTACAAAGTTTAGCCCAAACTAATAGTTCATTAATCACTCAGATGCCTCAAGTTGAGAAGTTCTATTCGGATCAAGGAATCATGGAATCAAATGGTGATTGCTTTATTGAGCAATTGCTTAATGTAGATGGATTCATTCAGAATAGTCGTGTGCCTTGGACAACACAATAA
Protein sequence

>MS.gene042263.t1

MMVVEDSFGVDDVLGEGFPAGMRVLAVDDDPTYLKVLEKQLLTCNYNVTTTTKSLEALELLREKKDMFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVIHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQGIISDGVYGQDTSSENIANKNKTHGQKRKVQSEEEEAEQDNDEKRSPQKKPRLVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQRYRIDIKWLSKQDRGDDALDPYQQKGSVGGYGDFCTLPAYASNDMFCRLNTPSSLNLEGMSSSALVPPLQSHNIPNFETPMFSESESSSSLHGIQTSMEINQFQQNIYPPDNINLSPIDDSNAVTDSSGFQDIRATVNNANVSLSCIASNHIQTHKSEAFINHSPVEGSAVKTQPFNPVRSGSSNFASNSVPLSEDFNNDQMLHNNYKFTSPSSHFSSTDIVAVPLEETMQCQDGLLGNVINAPCYSQHQNASSSFNNTLDYLASSNGDTSSMVQSLAQTNSSLITQMPQVEKFYSDQGIMESNGDCFIEQLLNVDGFIQNSRVPWTTQ