AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042268

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042268.t1 MTR_4g131570 91.060 604 36 4 1 594 1 596 0.0 1082
MS.gene042268.t1 MTR_4g131580 77.167 600 116 5 1 589 1 590 0.0 887
MS.gene042268.t1 MTR_4g131600 69.371 604 158 4 1 594 4 590 0.0 813
MS.gene042268.t1 MTR_2g450070 42.447 662 289 17 2 585 3 650 5.27e-141 424
MS.gene042268.t1 MTR_2g450070 42.447 662 289 17 2 585 3 650 2.20e-140 423
MS.gene042268.t1 MTR_3g106220 48.358 335 145 5 14 331 5 328 1.00e-92 298
MS.gene042268.t1 MTR_1g013170 46.988 332 161 3 15 331 3 334 6.70e-89 284
MS.gene042268.t1 MTR_4g121020 43.878 392 167 12 15 372 29 401 2.65e-83 275
MS.gene042268.t1 MTR_4g121020 43.878 392 167 12 15 372 29 401 2.76e-83 275
MS.gene042268.t1 MTR_2g034960 43.785 354 185 4 6 347 18 369 6.42e-81 266
MS.gene042268.t1 MTR_1g032570 46.078 306 125 4 16 294 10 302 1.15e-79 263
MS.gene042268.t1 MTR_1g013180 45.808 334 168 6 2 322 140 473 2.02e-79 264
MS.gene042268.t1 MTR_7g117705 52.653 245 110 3 15 253 3 247 9.34e-77 251
MS.gene042268.t1 MTR_3g086100 52.800 250 108 4 16 257 12 259 1.07e-74 249
MS.gene042268.t1 MTR_8g079940 51.220 246 116 1 17 258 25 270 1.49e-73 245
MS.gene042268.t1 MTR_3g102590 47.660 235 76 2 17 250 8 196 8.73e-57 191
MS.gene042268.t1 MTR_1g032570 45.122 246 95 4 76 294 1 233 2.03e-55 197
MS.gene042268.t1 MTR_3g102590 47.186 231 75 2 17 246 8 192 6.95e-54 182
MS.gene042268.t1 MTR_3g086100 51.309 191 81 5 76 257 1 188 4.44e-49 178
MS.gene042268.t1 MTR_4g121020 38.907 311 137 12 96 372 6 297 9.06e-45 167
MS.gene042268.t1 MTR_3g102600 60.000 130 52 0 11 140 2 131 3.99e-44 155
MS.gene042268.t1 MTR_3g102600 62.264 53 20 0 196 248 131 183 3.98e-15 74.3
MS.gene042268.t1 MTR_3g102600 60.000 130 52 0 11 140 2 131 6.67e-44 156
MS.gene042268.t1 MTR_3g102600 40.678 118 42 2 196 313 131 220 8.93e-20 89.0
MS.gene042268.t1 MTR_4g021790 35.656 244 135 4 17 260 10 231 2.68e-43 156
MS.gene042268.t1 MTR_4g021760 33.607 244 140 4 17 260 11 232 6.15e-43 156
MS.gene042268.t1 MTR_4g021855 34.426 244 138 4 17 260 10 231 2.92e-41 151
MS.gene042268.t1 MTR_0450s0040 36.441 236 129 5 21 253 8 225 6.09e-40 149
MS.gene042268.t1 MTR_4g021845 35.498 231 125 5 17 246 10 217 1.38e-38 142
MS.gene042268.t1 MTR_6g045327 32.677 254 144 4 1 253 1 228 1.20e-37 144
MS.gene042268.t1 MTR_1g013160 47.973 148 71 2 79 220 1 148 1.58e-35 131
MS.gene042268.t1 MTR_8g105600 34.109 258 136 4 13 254 5 244 1.29e-33 135
MS.gene042268.t1 MTR_7g026400 33.333 225 124 3 47 263 17 223 1.64e-30 126
MS.gene042268.t1 MTR_3g102600 62.195 82 31 0 59 140 1 82 2.26e-29 113
MS.gene042268.t1 MTR_3g102600 62.264 53 20 0 196 248 82 134 8.24e-16 75.1
MS.gene042268.t1 MTR_3g102600 62.195 82 31 0 59 140 1 82 4.90e-29 114
MS.gene042268.t1 MTR_3g102600 40.678 118 42 2 196 313 82 171 3.60e-20 88.6
MS.gene042268.t1 MTR_5g055260 31.513 238 114 6 17 254 10 198 6.07e-29 116
MS.gene042268.t1 MTR_8g032710 27.519 258 159 5 20 252 20 274 5.48e-25 108
MS.gene042268.t1 MTR_7g118260 31.892 185 114 3 18 196 11 189 1.12e-22 102
MS.gene042268.t1 MTR_4g064730 56.818 88 35 1 167 254 104 188 2.19e-22 98.6
MS.gene042268.t1 MTR_3g092780 29.778 225 128 8 21 227 56 268 4.09e-22 101
MS.gene042268.t1 MTR_3g092780 30.357 224 127 8 21 227 56 267 4.74e-22 100
MS.gene042268.t1 MTR_3g092780 29.778 225 128 8 21 227 56 268 4.87e-22 100
MS.gene042268.t1 MTR_8g093040 27.907 258 146 5 1 258 1 218 5.45e-22 96.7
MS.gene042268.t1 MTR_3g092780 30.357 224 127 8 21 227 56 267 5.65e-22 100
MS.gene042268.t1 MTR_8g024260 37.594 133 79 3 23 152 38 169 1.71e-21 99.4
MS.gene042268.t1 MTR_7g089010 55.000 80 36 0 175 254 69 148 4.28e-21 94.4
MS.gene042268.t1 MTR_7g118260 35.938 128 73 2 18 139 11 135 7.33e-21 97.1
MS.gene042268.t1 MTR_6g016850 34.058 138 88 2 13 148 5 141 1.42e-20 89.7
MS.gene042268.t1 MTR_4g023980 30.000 160 87 4 63 220 35 171 1.00e-19 87.4
MS.gene042268.t1 MTR_4g061360 36.691 139 81 4 5 139 75 210 1.78e-19 93.2
MS.gene042268.t1 MTR_1g067110 36.885 122 74 2 21 139 85 206 6.19e-19 91.3
MS.gene042268.t1 MTR_1g067110 36.885 122 74 2 21 139 85 206 7.09e-19 91.3
MS.gene042268.t1 MTR_3g037390 31.707 123 81 1 21 140 24 146 2.10e-17 86.3
MS.gene042268.t1 MTR_4g108880 31.707 123 81 1 21 140 33 155 3.92e-17 85.5
MS.gene042268.t1 MTR_4g098870 60.656 61 24 0 190 250 303 363 4.92e-16 81.6
MS.gene042268.t1 MTR_4g098870 31.361 169 109 3 15 182 12 174 3.51e-15 79.0
MS.gene042268.t1 MTR_4g098870 60.656 61 24 0 190 250 323 383 5.62e-16 81.6
MS.gene042268.t1 MTR_4g098870 31.361 169 109 3 15 182 32 194 5.44e-15 78.6
MS.gene042268.t1 MTR_4g098870 60.656 61 24 0 190 250 303 363 5.68e-16 81.6
MS.gene042268.t1 MTR_4g098870 31.361 169 109 3 15 182 12 174 6.06e-15 78.2
MS.gene042268.t1 MTR_4g098870 60.656 61 24 0 190 250 342 402 5.95e-16 81.6
MS.gene042268.t1 MTR_4g098870 31.361 169 109 3 15 182 51 213 4.70e-15 79.0
MS.gene042268.t1 MTR_4g098870 60.656 61 24 0 190 250 323 383 6.26e-16 81.6
MS.gene042268.t1 MTR_4g098870 31.361 169 109 3 15 182 32 194 7.66e-15 78.2
MS.gene042268.t1 MTR_5g014040 59.016 61 25 0 190 250 304 364 6.84e-16 81.3
MS.gene042268.t1 MTR_5g014040 37.302 126 75 2 15 139 12 134 9.37e-15 77.8
MS.gene042268.t1 MTR_5g014040 37.302 126 75 2 15 139 12 134 1.08e-14 77.4
MS.gene042268.t1 MTR_5g014040 54.545 66 25 1 190 250 304 369 6.29e-14 75.1
MS.gene042268.t1 MTR_1g093080 51.389 72 33 2 186 256 201 271 4.38e-14 74.7
MS.gene042268.t1 MTR_5g014040 54.545 66 25 1 190 250 264 329 5.32e-14 75.1
MS.gene042268.t1 MTR_7g490310 30.667 150 81 3 22 150 10 157 5.57e-13 69.3
MS.gene042268.t1 MTR_4g078810 50.909 55 27 0 195 249 156 210 6.16e-13 71.6
MS.gene042268.t1 MTR_2g090675 50.909 55 27 0 195 249 159 213 1.44e-12 70.5
MS.gene042268.t1 MTR_7g115530 53.226 62 28 1 195 255 216 277 1.47e-12 69.3
MS.gene042268.t1 MTR_8g038620 26.994 163 97 4 22 162 10 172 1.70e-12 66.6
MS.gene042268.t1 MTR_7g115530 53.226 62 28 1 195 255 216 277 2.35e-12 68.9
MS.gene042268.t1 MTR_1g049100 28.889 135 82 3 21 141 8 142 3.51e-12 66.2
MS.gene042268.t1 MTR_7g115530 53.226 62 28 1 195 255 216 277 3.82e-12 68.6
MS.gene042268.t1 MTR_1g080330 50.000 64 31 1 193 255 171 234 4.10e-12 67.8
MS.gene042268.t1 MTR_7g115530 53.226 62 28 1 195 255 216 277 4.82e-12 68.6
MS.gene042268.t1 MTR_1g080330 50.000 64 31 1 193 255 171 234 5.16e-12 68.2
MS.gene042268.t1 MTR_4g113140 46.479 71 37 1 194 263 232 302 5.87e-12 68.6
MS.gene042268.t1 MTR_7g098250 51.562 64 30 1 193 255 258 321 8.92e-12 68.2
MS.gene042268.t1 MTR_4g086835 49.180 61 31 0 194 254 271 331 1.22e-11 67.4
MS.gene042268.t1 MTR_6g444980 52.239 67 30 2 193 258 31 96 1.49e-11 67.0
MS.gene042268.t1 MTR_6g444980 52.239 67 30 2 193 258 31 96 1.50e-11 66.6
MS.gene042268.t1 MTR_4g106590 27.778 144 88 4 15 142 17 160 1.51e-11 64.7
MS.gene042268.t1 MTR_2g086450 53.333 60 27 1 193 251 41 100 2.07e-11 66.6
MS.gene042268.t1 MTR_6g088950 27.559 127 84 1 47 165 34 160 2.79e-11 63.9
MS.gene042268.t1 MTR_5g036480 26.994 163 102 4 21 166 16 178 3.02e-11 64.3
MS.gene042268.t1 MTR_6g032990 51.667 60 28 1 193 251 38 97 4.17e-11 65.5
MS.gene042268.t1 MTR_5g017980 49.153 59 30 0 196 254 191 249 4.47e-11 65.1
MS.gene042268.t1 MTR_5g017980 49.153 59 30 0 196 254 192 250 4.67e-11 64.7
MS.gene042268.t1 MTR_5g041350 52.542 59 27 1 193 250 11 69 4.98e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042268.t1 AT2G25180 44.862 399 172 10 1 371 1 379 4.42e-91 293
MS.gene042268.t1 AT4G31920 49.699 332 140 9 1 320 1 317 1.95e-84 274
MS.gene042268.t1 AT5G58080 45.753 365 182 7 9 366 6 361 2.14e-83 274
MS.gene042268.t1 AT3G16857 43.235 340 159 5 15 323 31 367 2.89e-79 263
MS.gene042268.t1 AT3G16857 43.235 340 159 5 15 323 31 367 4.66e-79 263
MS.gene042268.t1 AT1G67710 53.333 255 96 4 18 257 8 254 5.15e-70 235
MS.gene042268.t1 AT2G01760 47.148 263 120 3 13 259 3 262 2.15e-68 227
MS.gene042268.t1 AT3G16857 42.475 299 138 5 56 323 2 297 8.62e-65 224
MS.gene042268.t1 AT2G01760 42.907 289 118 4 15 259 5 290 1.34e-62 212
MS.gene042268.t1 AT5G49240 39.834 241 140 3 11 246 32 272 2.82e-48 170
MS.gene042268.t1 AT2G01760 48.000 200 85 4 76 259 1 197 1.16e-45 164
MS.gene042268.t1 AT1G67710 50.761 197 74 4 76 257 1 189 9.04e-45 165
MS.gene042268.t1 AT1G49190 32.468 308 179 10 17 312 30 320 1.73e-41 155
MS.gene042268.t1 AT3G62670 35.211 284 143 8 36 301 54 314 2.49e-40 152
MS.gene042268.t1 AT1G67710 46.591 176 71 4 97 257 13 180 5.32e-32 129
MS.gene042268.t1 AT5G07210 30.627 271 154 5 21 259 16 284 1.19e-30 127
MS.gene042268.t1 AT5G07210 29.749 279 154 6 21 259 16 292 2.76e-28 120
MS.gene042268.t1 AT2G27070 27.645 293 175 7 9 266 4 294 3.80e-26 113
MS.gene042268.t1 AT5G59570 54.945 91 41 0 164 254 108 198 3.96e-24 103
MS.gene042268.t1 AT5G59570 54.945 91 41 0 164 254 108 198 3.96e-24 103
MS.gene042268.t1 AT1G49190 38.400 125 77 0 17 141 30 154 5.88e-24 107
MS.gene042268.t1 AT1G49190 34.532 139 73 8 184 312 405 535 9.08e-11 65.1
MS.gene042268.t1 AT2G46790 37.209 129 78 2 21 146 37 165 1.38e-22 101
MS.gene042268.t1 AT3G46640 61.333 75 29 0 180 254 127 201 1.81e-22 99.0
MS.gene042268.t1 AT3G46640 61.333 75 29 0 180 254 127 201 1.81e-22 99.0
MS.gene042268.t1 AT2G46790 37.209 129 78 2 21 146 37 165 1.84e-22 101
MS.gene042268.t1 AT3G46640 61.333 75 29 0 180 254 127 201 2.37e-22 99.0
MS.gene042268.t1 AT5G24470 36.000 125 77 2 19 140 48 172 3.38e-22 101
MS.gene042268.t1 AT3G10760 61.429 70 27 0 185 254 93 162 2.39e-21 95.9
MS.gene042268.t1 AT5G05090 57.895 76 32 0 179 254 63 138 4.05e-21 94.0
MS.gene042268.t1 AT2G40970 54.545 77 35 0 178 254 86 162 2.97e-20 90.9
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 8.03e-20 92.4
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 8.03e-20 92.4
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 8.03e-20 92.4
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 1.41e-19 92.4
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 1.41e-19 92.4
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 1.41e-19 92.4
MS.gene042268.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.25e-19 92.0
MS.gene042268.t1 AT5G02810 36.885 122 74 2 21 139 78 199 1.10e-17 87.4
MS.gene042268.t1 AT5G61380 33.333 123 79 1 21 140 19 141 7.31e-17 84.7
MS.gene042268.t1 AT5G44190 58.333 60 25 0 190 249 140 199 3.17e-16 80.9
MS.gene042268.t1 AT5G44190 58.333 60 25 0 190 249 140 199 3.56e-16 81.3
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.15e-14 75.1
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 5.41e-13 72.0
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.15e-14 75.1
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 5.41e-13 72.0
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.15e-14 75.1
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 5.41e-13 72.0
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.15e-14 75.1
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 5.41e-13 72.0
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.15e-14 75.1
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 5.41e-13 72.0
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.15e-14 75.1
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 5.41e-13 72.0
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 7.76e-14 74.7
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 6.99e-13 71.6
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 7.76e-14 74.7
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 6.99e-13 71.6
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 7.76e-14 74.7
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 6.99e-13 71.6
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 7.76e-14 74.7
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 6.99e-13 71.6
MS.gene042268.t1 AT4G18020 39.394 132 71 3 15 139 12 141 7.76e-14 74.7
MS.gene042268.t1 AT4G18020 48.052 77 39 1 173 249 273 348 6.99e-13 71.6
MS.gene042268.t1 AT2G20570 45.000 80 42 1 170 249 128 205 3.49e-13 72.4
MS.gene042268.t1 AT2G03500 44.000 75 41 1 196 269 232 306 7.29e-13 71.2
MS.gene042268.t1 AT2G20570 62.500 48 18 0 202 249 174 221 2.05e-12 70.1
MS.gene042268.t1 AT1G79430 43.750 96 34 3 193 287 30 106 2.74e-12 68.9
MS.gene042268.t1 AT1G25550 45.333 75 38 2 185 256 195 269 3.98e-12 68.6
MS.gene042268.t1 AT5G45580 47.761 67 34 1 197 262 23 89 5.20e-12 67.4
MS.gene042268.t1 AT5G45580 47.761 67 34 1 197 262 7 73 5.46e-12 67.0
MS.gene042268.t1 AT2G01060 50.000 72 32 2 181 251 2 70 1.21e-11 66.2
MS.gene042268.t1 AT1G68670 42.169 83 42 2 175 256 198 275 1.45e-11 66.6
MS.gene042268.t1 AT3G04030 53.333 60 27 1 193 251 41 100 1.82e-11 66.6
MS.gene042268.t1 AT5G18240 55.000 60 26 1 193 251 41 100 1.87e-11 66.6
MS.gene042268.t1 AT5G18240 55.000 60 26 1 193 251 41 100 1.87e-11 66.6
MS.gene042268.t1 AT1G19050 27.389 157 99 3 1 142 1 157 1.88e-11 64.3
MS.gene042268.t1 AT3G04030 53.333 60 27 1 193 251 41 100 1.99e-11 66.6
MS.gene042268.t1 AT5G18240 55.000 60 26 1 193 251 41 100 1.99e-11 66.6
MS.gene042268.t1 AT5G18240 55.000 60 26 1 193 251 41 100 2.01e-11 66.6
MS.gene042268.t1 AT5G18240 55.000 60 26 1 193 251 41 100 2.01e-11 66.6
MS.gene042268.t1 AT1G10470 30.000 130 79 3 21 138 20 149 3.91e-11 63.9
MS.gene042268.t1 AT1G10470 30.000 130 79 3 21 138 34 163 4.42e-11 64.3
MS.gene042268.t1 AT3G57040 25.949 158 95 2 22 157 10 167 5.26e-11 63.5
MS.gene042268.t1 AT3G57040 25.949 158 95 2 22 157 10 167 5.26e-11 63.5

Find 116 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCGCTCAGTTCTCAATTT+AGG 0.173828 8.2:+3660710 MS.gene042268:CDS
AGCTTGAATCCATCCATTTC+TGG 0.203555 8.2:-3659001 None:intergenic
TTATAGTTTGTACCTGTAAA+TGG 0.224166 8.2:-3659793 None:intergenic
TGAAAACTCGAGTGCTTTAA+AGG 0.227005 8.2:+3660336 MS.gene042268:CDS
AAACAATGGCTGCTTGTAAC+TGG 0.250042 8.2:-3660305 None:intergenic
CGTCATTGATGTTCCTTTAG+AGG 0.255703 8.2:+3660762 MS.gene042268:CDS
TATTAGCTTGGGGTACATTA+TGG 0.266193 8.2:-3660924 None:intergenic
GGAAGAAGCCTCGTTTAGTT+TGG 0.283283 8.2:+3659498 MS.gene042268:CDS
TCCTGTGGGAATGCATGTTC+TGG 0.298859 8.2:+3657866 MS.gene042268:CDS
CTTCTCATGTGCAAGTATAA+TGG 0.318350 8.2:+3657933 MS.gene042268:CDS
CAATGTCAAGATGGATTACT+TGG 0.334290 8.2:+3660793 MS.gene042268:CDS
GAGAAAGAAGATTGAAAGTA+AGG 0.340472 8.2:+3659295 MS.gene042268:CDS
TGAGGCATCTGAGTGATTAA+TGG 0.341513 8.2:-3660949 None:intergenic
CATTGCATTGTTTCCTCTAA+AGG 0.359272 8.2:-3660775 None:intergenic
AAGAATTCAGTAGCTGGATA+TGG 0.366836 8.2:+3660121 MS.gene042268:CDS
AGTGCATTTACAGATTCTTC+TGG 0.369412 8.2:+3660445 MS.gene042268:CDS
GAAGAAGCCTCGTTTAGTTT+GGG 0.371171 8.2:+3659499 MS.gene042268:CDS
TTATTCAGATGAAAGAACTA+TGG 0.373116 8.2:+3660984 MS.gene042268:CDS
AGGACTGGAAGCAAAGTTAT+AGG 0.378746 8.2:-3660629 None:intergenic
GATGGCTTTGGAGAATCCTT+TGG 0.383986 8.2:+3657815 None:intergenic
TCTCAAAAGGCCATCTAAAC+AGG 0.387755 8.2:+3660063 MS.gene042268:CDS
GATAAATGTTCCGGTGAAAC+TGG 0.391840 8.2:-3660384 None:intergenic
TCAGTAATGATACCTTTATT+CGG 0.392934 8.2:-3659320 None:intergenic
AAATGTGGCTAGCCATTTAC+AGG 0.398143 8.2:+3659781 MS.gene042268:CDS
CTCTTGAATTATGTGTTTGT+AGG 0.401274 8.2:-3660534 None:intergenic
TCTACTCTAGCCTGTTTAGA+TGG 0.401513 8.2:-3660073 None:intergenic
ATTCTGGTTCATGATGGCTT+TGG 0.405441 8.2:+3657803 None:intergenic
GAAAGTAAGGATCCGAATAA+AGG 0.416092 8.2:+3659308 MS.gene042268:CDS
TTCAGAATAGTTGTTTGCCT+TGG 0.419816 8.2:+3661061 MS.gene042268:CDS
CTTAAGATTCTTCTTGATCC+AGG 0.420041 8.2:-3660160 None:intergenic
TGTCGGCTGAACGCACCATC+TGG 0.422911 8.2:+3660220 MS.gene042268:CDS
TGAGCAAGTTGGACATGAAA+TGG 0.425491 8.2:+3659027 MS.gene042268:CDS
GAGCAATTGCTTGATGTAGA+TGG 0.429100 8.2:+3661033 MS.gene042268:CDS
TTTATAAATCACCCATCTGT+TGG 0.434200 8.2:+3660559 MS.gene042268:CDS
AATGCACTTGAATCATCAAT+TGG 0.435178 8.2:-3660430 None:intergenic
GATTAATGGACTATTAGCTT+GGG 0.435758 8.2:-3660935 None:intergenic
GAAAACGCAGAAGAAGATAA+TGG 0.437835 8.2:+3659446 MS.gene042268:CDS
TACCAGGTGGATAAATGTTC+CGG 0.439267 8.2:-3660393 None:intergenic
CATCTAGTAACATGTATTGT+CGG 0.449068 8.2:+3660203 MS.gene042268:CDS
TCTCTTGCTTCTTCTAATGC+AGG 0.449172 8.2:+3660886 MS.gene042268:CDS
TAACATTAGCTCTGATGTCT+TGG 0.449900 8.2:-3660471 None:intergenic
CACCGGAACATTTATCCACC+TGG 0.455890 8.2:+3660391 MS.gene042268:CDS
TTGGAGGTTGAAGATGTCTT+CGG 0.457510 8.2:+3657834 MS.gene042268:CDS
TTATAGGAACCACTTGTAGC+AGG 0.464041 8.2:-3660613 None:intergenic
AATACAAACTTAGTCTCAAA+AGG 0.473606 8.2:+3660050 MS.gene042268:CDS
TGGAAGAGCTTAAGAACATA+TGG 0.475204 8.2:+3659261 MS.gene042268:CDS
GGAGATTTGTGTACCTTACC+TGG 0.477382 8.2:+3660142 MS.gene042268:CDS
TGATTAATGGACTATTAGCT+TGG 0.477988 8.2:-3660936 None:intergenic
TGATGTAAACATGCCAGAAA+TGG 0.478571 8.2:+3658988 MS.gene042268:CDS
TTCTTCCTTCAACAATACCT+TGG 0.480791 8.2:+3660861 MS.gene042268:CDS
TTAAGCTCTTCCAATCGGAT+TGG 0.487989 8.2:-3659251 None:intergenic
GATAGAGAGAGGGTGATGAA+AGG 0.489158 8.2:+3659194 MS.gene042268:CDS
TCAAACTTGTCTCAAACTGT+TGG 0.489304 8.2:+3657902 MS.gene042268:CDS
TGTTCTTAAGCTCTTCCAAT+CGG 0.493421 8.2:-3659256 None:intergenic
GGCTTTGGAGAATCCTTTGG+AGG 0.494937 8.2:+3657818 MS.gene042268:CDS
CTCACTCAATGGAGAAGGAC+TGG 0.495705 8.2:-3660644 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.2:+3659756 MS.gene042268:CDS
GAAAGAACTATGGAATCAAA+TGG 0.497837 8.2:+3660994 MS.gene042268:CDS
CAGTCCTTCTCCATTGAGTG+AGG 0.499289 8.2:+3660645 MS.gene042268:CDS
CTACAAAAGAATTCAGTAGC+TGG 0.500934 8.2:+3660115 MS.gene042268:CDS
ATCTGTTGGAGGTCCTGCTA+TGG 0.503540 8.2:+3660573 MS.gene042268:CDS
GAAAACTCGAGTGCTTTAAA+GGG 0.504861 8.2:+3660337 MS.gene042268:CDS
AGAATCCAAGGTATTGTTGA+AGG 0.506294 8.2:-3660866 None:intergenic
TTATTAAAATCCTCACTCAA+TGG 0.507785 8.2:-3660655 None:intergenic
CAGGAAATGATGATAGAGAG+AGG 0.513398 8.2:+3659183 MS.gene042268:CDS
TGTGATCATATAGTGTTGTC+AGG 0.513792 8.2:+3659164 MS.gene042268:intron
AGAAGAAGCAAGAGAATCCA+AGG 0.514940 8.2:-3660878 None:intergenic
GGACTTAAGTTCATGTTACC+AGG 0.516544 8.2:-3660409 None:intergenic
TTCAAGCTCCTTGAGCAAGT+TGG 0.519096 8.2:+3659016 MS.gene042268:CDS
GATTCTTCTTGATCCAGGTA+AGG 0.519260 8.2:-3660155 None:intergenic
AACAATGGCTGCTTGTAACT+GGG 0.528436 8.2:-3660304 None:intergenic
GAATAAAATTTCTCAACTTG+AGG 0.529905 8.2:-3660967 None:intergenic
GTCACATAAACTCACTATCA+AGG 0.536123 8.2:-3659568 None:intergenic
TTCACTTATTGTATCATCCA+AGG 0.536142 8.2:-3661078 None:intergenic
GAAACAATGCAATGTCAAGA+TGG 0.544924 8.2:+3660784 MS.gene042268:CDS
TCTTGTGAAACCAATCCGAT+TGG 0.545613 8.2:+3659241 MS.gene042268:CDS
TGTGACTTGATGAATGTTGA+AGG 0.547972 8.2:+3659746 MS.gene042268:CDS
ATAAATCACCCATCTGTTGG+AGG 0.552382 8.2:+3660562 MS.gene042268:CDS
TTGAAGCGCTAGAATTGTTG+AGG 0.553092 8.2:+3658933 MS.gene042268:CDS
GTGTGTGAGCTTACTGATGA+AGG 0.556710 8.2:-3659056 None:intergenic
TAATGCAGGCACTAGTTCCA+TGG 0.559067 8.2:+3660900 MS.gene042268:CDS
TGAAGCGCTAGAATTGTTGA+GGG 0.561694 8.2:+3658934 MS.gene042268:CDS
ATGATGAGGAACGCTCGACT+CGG 0.562718 8.2:+3659477 MS.gene042268:CDS
AGGAAATGATGATAGAGAGA+GGG 0.563967 8.2:+3659184 MS.gene042268:CDS
TTTCATGTCCAACTTGCTCA+AGG 0.564395 8.2:-3659024 None:intergenic
TGGAAATTAACCAGTTTCAC+CGG 0.564960 8.2:+3660374 MS.gene042268:CDS
ATGTTACTAGATGCATATGT+TGG 0.572114 8.2:-3660193 None:intergenic
AAATCCTCACTCAATGGAGA+AGG 0.576468 8.2:-3660649 None:intergenic
ATAGCAGGACCTCCAACAGA+TGG 0.581262 8.2:-3660571 None:intergenic
CCATCTGGATTGAACTTGAG+AGG 0.585944 8.2:+3660235 MS.gene042268:CDS
TATAGGAACCACTTGTAGCA+GGG 0.588786 8.2:-3660612 None:intergenic
AAAGGGTGTTCGAACGTCAG+TGG 0.592037 8.2:+3660354 MS.gene042268:CDS
CCTCTCAAGTTCAATCCAGA+TGG 0.601365 8.2:-3660235 None:intergenic
GTAAACATGCCAGAAATGGA+TGG 0.605525 8.2:+3658992 MS.gene042268:CDS
GTGATGAAAGGAGTGATGAA+AGG 0.606414 8.2:+3659206 MS.gene042268:CDS
TAGCAGGACCTCCAACAGAT+GGG 0.607030 8.2:-3660570 None:intergenic
AAGACATCTTCAACCTCCAA+AGG 0.613959 8.2:-3657831 None:intergenic
CCTAAATTGAGAACTGAGCG+AGG 0.617995 8.2:-3660710 None:intergenic
GCCAGAACATGCATTCCCAC+AGG 0.618067 8.2:-3657867 None:intergenic
AGGGCTTACAAGGGAAAATG+TGG 0.634196 8.2:+3659766 MS.gene042268:CDS
ATTAATGGACTATTAGCTTG+GGG 0.634710 8.2:-3660934 None:intergenic
GTATCTATAGTTAGTCAACT+CGG 0.636343 8.2:+3659545 MS.gene042268:CDS
TAATGGTAGAGAGAATGATG+AGG 0.638904 8.2:+3659463 MS.gene042268:CDS
ATTACTTGGGAATGTTGTAG+CGG 0.641596 8.2:+3660807 MS.gene042268:CDS
TGAGCTTACTGATGAAGGGT+AGG 0.644624 8.2:-3659051 None:intergenic
GTGACTTGATGAATGTTGAA+GGG 0.645884 8.2:+3659747 MS.gene042268:CDS
AATGTCAAGATGGATTACTT+GGG 0.659008 8.2:+3660794 MS.gene042268:CDS
TGTGTGAGCTTACTGATGAA+GGG 0.665662 8.2:-3659055 None:intergenic
CTTAAGTTCATGTTACCAGG+TGG 0.668465 8.2:-3660406 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.2:+3659757 MS.gene042268:CDS
TTACACGCAGCAGCAAAACG+TGG 0.681302 8.2:+3660837 MS.gene042268:CDS
TCATCATCCCAAACTAAACG+AGG 0.691444 8.2:-3659506 None:intergenic
TTGGGGTACATTATGGACCA+TGG 0.694419 8.2:-3660917 None:intergenic
GCTAACAAGAATAAAACCCG+TGG 0.697003 8.2:+3659380 MS.gene042268:CDS
TACACGCAGCAGCAAAACGT+GGG 0.705438 8.2:+3660838 MS.gene042268:CDS
AGAACTATGGAATCAAATGG+TGG 0.707711 8.2:+3660997 MS.gene042268:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGAAATATTTTGACAAAA+GGG - chr8.2:3659823-3659842 None:intergenic 15.0%
!!! TTATGTTTCTACTAATTTTT+TGG - chr8.2:3658389-3658408 None:intergenic 15.0%
!! AAAATGAGAAAACAGTATTT+AGG - chr8.2:3658657-3658676 None:intergenic 20.0%
!! AAAGTCGAAAATAAAAATAG+AGG + chr8.2:3658519-3658538 MS.gene042268:intron 20.0%
!! ATTCACTTCTTATATTCATA+TGG + chr8.2:3659115-3659134 MS.gene042268:intron 20.0%
!! TAGGTCAACATATATTATTA+GGG - chr8.2:3658347-3658366 None:intergenic 20.0%
!!! AAATATTTTCGATGTAATCT+TGG + chr8.2:3658264-3658283 MS.gene042268:intron 20.0%
!!! AATAATTCAGTTGTTTTGAT+TGG + chr8.2:3658556-3658575 MS.gene042268:intron 20.0%
!!! ATAAGATCACTACTTTTATT+GGG + chr8.2:3658758-3658777 MS.gene042268:intron 20.0%
!!! ATGAAATATTTTGACAAAAG+GGG - chr8.2:3659822-3659841 None:intergenic 20.0%
!!! GAGAGACATTTAAAATAAAA+AGG - chr8.2:3658858-3658877 None:intergenic 20.0%
!!! GTATGAAATATTTTGACAAA+AGG - chr8.2:3659824-3659843 None:intergenic 20.0%
!!! TAGTAAACCCTTTTTTTAAT+CGG + chr8.2:3658082-3658101 MS.gene042268:intron 20.0%
!!! TGTTACTTTACTAATTTGAT+TGG + chr8.2:3659605-3659624 MS.gene042268:intron 20.0%
! AAAAATAAAGGAACAAAGTG+AGG + chr8.2:3659406-3659425 MS.gene042268:CDS 25.0%
! AATACAAACTTAGTCTCAAA+AGG + chr8.2:3660050-3660069 MS.gene042268:CDS 25.0%
! ACCCGAAATAAACAAAAAAT+TGG - chr8.2:3658295-3658314 None:intergenic 25.0%
! CCCGAAATAAACAAAAAATT+GGG - chr8.2:3658294-3658313 None:intergenic 25.0%
! CCGAAATAAACAAAAAATTG+GGG - chr8.2:3658293-3658312 None:intergenic 25.0%
! CTGAAAGAAAGAAGAAAAAA+TGG - chr8.2:3657966-3657985 None:intergenic 25.0%
! GAATAAAATTTCTCAACTTG+AGG - chr8.2:3660970-3660989 None:intergenic 25.0%
! GTAGGTCAACATATATTATT+AGG - chr8.2:3658348-3658367 None:intergenic 25.0%
! GTTGAAATTTCAACGAATAA+TGG - chr8.2:3659965-3659984 None:intergenic 25.0%
! TAATACACATGCTAAAACAA+AGG - chr8.2:3659081-3659100 None:intergenic 25.0%
! TCAGTAATGATACCTTTATT+CGG - chr8.2:3659323-3659342 None:intergenic 25.0%
! TGATGATTGAAACTTACATA+TGG + chr8.2:3659659-3659678 MS.gene042268:intron 25.0%
! TGTAAGTTTCAATCATCAAA+TGG - chr8.2:3659657-3659676 None:intergenic 25.0%
! TTATTAAAATCCTCACTCAA+TGG - chr8.2:3660658-3660677 None:intergenic 25.0%
! TTATTCAGATGAAAGAACTA+TGG + chr8.2:3660984-3661003 MS.gene042268:CDS 25.0%
! TTTGGCAAATATACTTCTTT+GGG - chr8.2:3658371-3658390 None:intergenic 25.0%
!! ATCAAGTCACATATTTTCTT+AGG - chr8.2:3659737-3659756 None:intergenic 25.0%
!! CTCTTTTATGAAACATAAGT+GGG + chr8.2:3659988-3660007 MS.gene042268:intron 25.0%
!! TAACTTATAAGCTTTGTAGT+CGG + chr8.2:3658180-3658199 MS.gene042268:intron 25.0%
!! TCTCTTTTATGAAACATAAG+TGG + chr8.2:3659987-3660006 MS.gene042268:intron 25.0%
!! TTATAGTTTGTACCTGTAAA+TGG - chr8.2:3659796-3659815 None:intergenic 25.0%
!! TTTTCACTTGCAAAAAACAA+TGG - chr8.2:3660322-3660341 None:intergenic 25.0%
!! TTTTGGCAAATATACTTCTT+TGG - chr8.2:3658372-3658391 None:intergenic 25.0%
!!! AATAAAGGTATCATTACTGA+TGG + chr8.2:3659323-3659342 MS.gene042268:intron 25.0%
!!! AGTAAACCCTTTTTTTAATC+GGG + chr8.2:3658083-3658102 MS.gene042268:intron 25.0%
!!! ATAAAGGTATCATTACTGAT+GGG + chr8.2:3659324-3659343 MS.gene042268:intron 25.0%
!!! ATTTGATTGGCTTTAAAACA+TGG + chr8.2:3659618-3659637 MS.gene042268:intron 25.0%
!!! CATAAGATCACTACTTTTAT+TGG + chr8.2:3658757-3658776 MS.gene042268:intron 25.0%
!!! CCCAATTTTTTGTTTATTTC+GGG + chr8.2:3658291-3658310 MS.gene042268:intron 25.0%
!!! CCCCAATTTTTTGTTTATTT+CGG + chr8.2:3658290-3658309 MS.gene042268:intron 25.0%
AAATATCGTCGAAGTTAAAG+GGG + chr8.2:3659882-3659901 MS.gene042268:intron 30.0%
AACTTTGCTAGCAATGAAAA+TGG + chr8.2:3658489-3658508 MS.gene042268:intron 30.0%
AATGCACTTGAATCATCAAT+TGG - chr8.2:3660433-3660452 None:intergenic 30.0%
ATGTTACTAGATGCATATGT+TGG - chr8.2:3660196-3660215 None:intergenic 30.0%
ATTTGCCCCGATTAAAAAAA+GGG - chr8.2:3658092-3658111 None:intergenic 30.0%
CAAATATCGTCGAAGTTAAA+GGG + chr8.2:3659881-3659900 MS.gene042268:intron 30.0%
CAGTGGTGTAAACTTATAAT+TGG + chr8.2:3658045-3658064 MS.gene042268:intron 30.0%
CATATGCTTGTATGCAAATA+TGG + chr8.2:3659921-3659940 MS.gene042268:intron 30.0%
CATCTAGTAACATGTATTGT+CGG + chr8.2:3660203-3660222 MS.gene042268:CDS 30.0%
CTCTTGAATTATGTGTTTGT+AGG - chr8.2:3660537-3660556 None:intergenic 30.0%
GAAAGAACTATGGAATCAAA+TGG + chr8.2:3660994-3661013 MS.gene042268:CDS 30.0%
GAGAAAGAAGATTGAAAGTA+AGG + chr8.2:3659295-3659314 MS.gene042268:CDS 30.0%
GTATCTATAGTTAGTCAACT+CGG + chr8.2:3659545-3659564 MS.gene042268:CDS 30.0%
GTCAACATATATTATTAGGG+TGG - chr8.2:3658344-3658363 None:intergenic 30.0%
TTGGCAAATATACTTCTTTG+GGG - chr8.2:3658370-3658389 None:intergenic 30.0%
TTTATAAATCACCCATCTGT+TGG + chr8.2:3660559-3660578 MS.gene042268:CDS 30.0%
! AAAGATTTTTGTTCCATAGC+AGG - chr8.2:3660589-3660608 None:intergenic 30.0%
! AGTTGAAGAAAGTACATGTT+AGG - chr8.2:3659696-3659715 None:intergenic 30.0%
! GTTAGCAATATTTTCAGATG+AGG - chr8.2:3659366-3659385 None:intergenic 30.0%
!! AATGTCAAGATGGATTACTT+GGG + chr8.2:3660794-3660813 MS.gene042268:CDS 30.0%
!! ATCATCAAATGGTCTTGTTA+AGG - chr8.2:3659646-3659665 None:intergenic 30.0%
!! ATTAATGGACTATTAGCTTG+GGG - chr8.2:3660937-3660956 None:intergenic 30.0%
!! GATTAATGGACTATTAGCTT+GGG - chr8.2:3660938-3660957 None:intergenic 30.0%
!! TGATTAATGGACTATTAGCT+TGG - chr8.2:3660939-3660958 None:intergenic 30.0%
!!! GTAAACCCTTTTTTTAATCG+GGG + chr8.2:3658084-3658103 MS.gene042268:intron 30.0%
!!! GTTCCTTTATTTTTTGTCCA+CGG - chr8.2:3659400-3659419 None:intergenic 30.0%
!!! TTCCTTTATTTTTTGTCCAC+GGG - chr8.2:3659399-3659418 None:intergenic 30.0%
AACCCGTGGACAAAAAATAA+AGG + chr8.2:3659394-3659413 MS.gene042268:CDS 35.0%
AAGAATTCAGTAGCTGGATA+TGG + chr8.2:3660121-3660140 MS.gene042268:CDS 35.0%
AGAACTATGGAATCAAATGG+TGG + chr8.2:3660997-3661016 MS.gene042268:CDS 35.0%
AGGAAATGATGATAGAGAGA+GGG + chr8.2:3659184-3659203 MS.gene042268:CDS 35.0%
AGTGCATTTACAGATTCTTC+TGG + chr8.2:3660445-3660464 MS.gene042268:CDS 35.0%
ATTACTTGGGAATGTTGTAG+CGG + chr8.2:3660807-3660826 MS.gene042268:CDS 35.0%
CATTGCATTGTTTCCTCTAA+AGG - chr8.2:3660778-3660797 None:intergenic 35.0%
CATTTGCCCCGATTAAAAAA+AGG - chr8.2:3658093-3658112 None:intergenic 35.0%
CGACGATATTTGCAAAAATG+TGG - chr8.2:3659873-3659892 None:intergenic 35.0%
CTACAAAAGAATTCAGTAGC+TGG + chr8.2:3660115-3660134 MS.gene042268:CDS 35.0%
CTTAAGATTCTTCTTGATCC+AGG - chr8.2:3660163-3660182 None:intergenic 35.0%
CTTCTCATGTGCAAGTATAA+TGG + chr8.2:3657933-3657952 MS.gene042268:CDS 35.0%
GAAAACGCAGAAGAAGATAA+TGG + chr8.2:3659446-3659465 MS.gene042268:CDS 35.0%
GAAACAATGCAATGTCAAGA+TGG + chr8.2:3660784-3660803 MS.gene042268:CDS 35.0%
GAAAGTAAGGATCCGAATAA+AGG + chr8.2:3659308-3659327 MS.gene042268:CDS 35.0%
GCAAATATCGTCGAAGTTAA+AGG + chr8.2:3659880-3659899 MS.gene042268:intron 35.0%
GTCACATAAACTCACTATCA+AGG - chr8.2:3659571-3659590 None:intergenic 35.0%
GTGACTTGATGAATGTTGAA+GGG + chr8.2:3659747-3659766 MS.gene042268:CDS 35.0%
GTGTCGAAGAAAAATCAGTT+GGG - chr8.2:3660741-3660760 None:intergenic 35.0%
TAATGGTAGAGAGAATGATG+AGG + chr8.2:3659463-3659482 MS.gene042268:CDS 35.0%
TCAAACTTGTCTCAAACTGT+TGG + chr8.2:3657902-3657921 MS.gene042268:CDS 35.0%
TGAAAACTCGAGTGCTTTAA+AGG + chr8.2:3660336-3660355 MS.gene042268:CDS 35.0%
TGAATCACTACTTTGATGCT+TGG + chr8.2:3658880-3658899 MS.gene042268:intron 35.0%
TGATGTAAACATGCCAGAAA+TGG + chr8.2:3658988-3659007 MS.gene042268:CDS 35.0%
TGATTGAATGTGACATGTGT+CGG - chr8.2:3658232-3658251 None:intergenic 35.0%
TGCTAGCAATGAAAATGGTA+AGG + chr8.2:3658494-3658513 MS.gene042268:intron 35.0%
TGGAAATTAACCAGTTTCAC+CGG + chr8.2:3660374-3660393 MS.gene042268:CDS 35.0%
TGGAAGAGCTTAAGAACATA+TGG + chr8.2:3659261-3659280 MS.gene042268:CDS 35.0%
TGTGACTTGATGAATGTTGA+AGG + chr8.2:3659746-3659765 MS.gene042268:CDS 35.0%
TGTGATCATATAGTGTTGTC+AGG + chr8.2:3659164-3659183 MS.gene042268:intron 35.0%
TGTTCTTAAGCTCTTCCAAT+CGG - chr8.2:3659259-3659278 None:intergenic 35.0%
TTCAGAATAGTTGTTTGCCT+TGG + chr8.2:3661061-3661080 MS.gene042268:CDS 35.0%
TTCTTCAACTGTTATTGCAG+AGG + chr8.2:3659706-3659725 MS.gene042268:intron 35.0%
TTCTTCCTTCAACAATACCT+TGG + chr8.2:3660861-3660880 MS.gene042268:CDS 35.0%
! ACTGAATTCTTTTGTAGGTG+AGG - chr8.2:3660112-3660131 None:intergenic 35.0%
! CAGCTACTGAATTCTTTTGT+AGG - chr8.2:3660117-3660136 None:intergenic 35.0%
! GAAAACTCGAGTGCTTTAAA+GGG + chr8.2:3660337-3660356 MS.gene042268:CDS 35.0%
! TATTAGCTTGGGGTACATTA+TGG - chr8.2:3660927-3660946 None:intergenic 35.0%
!! AGAATCCAAGGTATTGTTGA+AGG - chr8.2:3660869-3660888 None:intergenic 35.0%
!! CAAATATACTTCTTTGGGGT+AGG - chr8.2:3658366-3658385 None:intergenic 35.0%
!! CAATGTCAAGATGGATTACT+TGG + chr8.2:3660793-3660812 MS.gene042268:CDS 35.0%
!! TAACATTAGCTCTGATGTCT+TGG - chr8.2:3660474-3660493 None:intergenic 35.0%
!!! TCGTCTTGTTTTGACATTTC+TGG + chr8.2:3658444-3658463 MS.gene042268:intron 35.0%
AAACAATGGCTGCTTGTAAC+TGG - chr8.2:3660308-3660327 None:intergenic 40.0%
AAAGGCTTCCTGCGTTATAA+AGG - chr8.2:3658840-3658859 None:intergenic 40.0%
AAATCCTCACTCAATGGAGA+AGG - chr8.2:3660652-3660671 None:intergenic 40.0%
AACAATGGCTGCTTGTAACT+GGG - chr8.2:3660307-3660326 None:intergenic 40.0%
AAGACATCTTCAACCTCCAA+AGG - chr8.2:3657834-3657853 None:intergenic 40.0%
AAGTTATAACCAAGACGTCG+TGG - chr8.2:3658166-3658185 None:intergenic 40.0%
AGAAGAAGCAAGAGAATCCA+AGG - chr8.2:3660881-3660900 None:intergenic 40.0%
AGCTTGAATCCATCCATTTC+TGG - chr8.2:3659004-3659023 None:intergenic 40.0%
AGGACTGGAAGCAAAGTTAT+AGG - chr8.2:3660632-3660651 None:intergenic 40.0%
ATAAATCACCCATCTGTTGG+AGG + chr8.2:3660562-3660581 MS.gene042268:CDS 40.0%
CAGGAAATGATGATAGAGAG+AGG + chr8.2:3659183-3659202 MS.gene042268:CDS 40.0%
CGTCATTGATGTTCCTTTAG+AGG + chr8.2:3660762-3660781 MS.gene042268:CDS 40.0%
CGTGTCGAAGAAAAATCAGT+TGG - chr8.2:3660742-3660761 None:intergenic 40.0%
CTTAAGTTCATGTTACCAGG+TGG - chr8.2:3660409-3660428 None:intergenic 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.2:3659757-3659776 MS.gene042268:CDS 40.0%
GAGCAATTGCTTGATGTAGA+TGG + chr8.2:3661033-3661052 MS.gene042268:CDS 40.0%
GATAAATGTTCCGGTGAAAC+TGG - chr8.2:3660387-3660406 None:intergenic 40.0%
GATTCTTCTTGATCCAGGTA+AGG - chr8.2:3660158-3660177 None:intergenic 40.0%
GCTAACAAGAATAAAACCCG+TGG + chr8.2:3659380-3659399 MS.gene042268:CDS 40.0%
GGACTTAAGTTCATGTTACC+AGG - chr8.2:3660412-3660431 None:intergenic 40.0%
GTAAACATGCCAGAAATGGA+TGG + chr8.2:3658992-3659011 MS.gene042268:CDS 40.0%
GTGATGAAAGGAGTGATGAA+AGG + chr8.2:3659206-3659225 MS.gene042268:CDS 40.0%
TACCAGGTGGATAAATGTTC+CGG - chr8.2:3660396-3660415 None:intergenic 40.0%
TATAGGAACCACTTGTAGCA+GGG - chr8.2:3660615-3660634 None:intergenic 40.0%
TCATCATCCCAAACTAAACG+AGG - chr8.2:3659509-3659528 None:intergenic 40.0%
TCTACTCTAGCCTGTTTAGA+TGG - chr8.2:3660076-3660095 None:intergenic 40.0%
TCTCAAAAGGCCATCTAAAC+AGG + chr8.2:3660063-3660082 MS.gene042268:CDS 40.0%
TCTCTTGCTTCTTCTAATGC+AGG + chr8.2:3660886-3660905 MS.gene042268:CDS 40.0%
TCTTGTGAAACCAATCCGAT+TGG + chr8.2:3659241-3659260 MS.gene042268:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.2:3659756-3659775 MS.gene042268:CDS 40.0%
TGCATGAGAGGAGAATAGTA+CGG - chr8.2:3658027-3658046 None:intergenic 40.0%
TGGGAACAATAGACCTCATT+GGG + chr8.2:3658802-3658821 MS.gene042268:intron 40.0%
TGTGTGAGCTTACTGATGAA+GGG - chr8.2:3659058-3659077 None:intergenic 40.0%
TTAAGCTCTTCCAATCGGAT+TGG - chr8.2:3659254-3659273 None:intergenic 40.0%
TTATAGGAACCACTTGTAGC+AGG - chr8.2:3660616-3660635 None:intergenic 40.0%
TTGACTGTCCTTTATAACGC+AGG + chr8.2:3658829-3658848 MS.gene042268:intron 40.0%
TTGGAGGTTGAAGATGTCTT+CGG + chr8.2:3657834-3657853 MS.gene042268:CDS 40.0%
! AAAGACACTGCTAGTCTGTT+TGG + chr8.2:3658580-3658599 MS.gene042268:intron 40.0%
! AAATGTGGCTAGCCATTTAC+AGG + chr8.2:3659781-3659800 MS.gene042268:CDS 40.0%
! GAAGAAGCCTCGTTTAGTTT+GGG + chr8.2:3659499-3659518 MS.gene042268:CDS 40.0%
! GACACATTAGTGTTGGAAGT+GGG + chr8.2:3658783-3658802 MS.gene042268:intron 40.0%
! TCTTTTAACCCTGCTACAAG+TGG + chr8.2:3660604-3660623 MS.gene042268:CDS 40.0%
! TGAAGCGCTAGAATTGTTGA+GGG + chr8.2:3658934-3658953 MS.gene042268:CDS 40.0%
! TGACACATTAGTGTTGGAAG+TGG + chr8.2:3658782-3658801 MS.gene042268:intron 40.0%
! TGAGCAAGTTGGACATGAAA+TGG + chr8.2:3659027-3659046 MS.gene042268:CDS 40.0%
! TGAGGCATCTGAGTGATTAA+TGG - chr8.2:3660952-3660971 None:intergenic 40.0%
! TTCGGAGAAGATTTTCCTGT+GGG + chr8.2:3657852-3657871 MS.gene042268:CDS 40.0%
! TTGAAGCGCTAGAATTGTTG+AGG + chr8.2:3658933-3658952 MS.gene042268:CDS 40.0%
! TTTCATGTCCAACTTGCTCA+AGG - chr8.2:3659027-3659046 None:intergenic 40.0%
!!! ATGTTTTGAGACTGAAGTGG+AGG - chr8.2:3660283-3660302 None:intergenic 40.0%
!!! GGAATGTTTTGAGACTGAAG+TGG - chr8.2:3660286-3660305 None:intergenic 40.0%
!!! TGTTTTGAGACTGAAGTGGA+GGG - chr8.2:3660282-3660301 None:intergenic 40.0%
ACTATTCTCCTCTCATGCAG+TGG + chr8.2:3658028-3658047 MS.gene042268:intron 45.0%
AGGGCTTACAAGGGAAAATG+TGG + chr8.2:3659766-3659785 MS.gene042268:CDS 45.0%
AGTTTACACCACTGCATGAG+AGG - chr8.2:3658039-3658058 None:intergenic 45.0%
CCATCTGGATTGAACTTGAG+AGG + chr8.2:3660235-3660254 MS.gene042268:CDS 45.0%
CCTAAATTGAGAACTGAGCG+AGG - chr8.2:3660713-3660732 None:intergenic 45.0%
CCTCGCTCAGTTCTCAATTT+AGG + chr8.2:3660710-3660729 MS.gene042268:CDS 45.0%
CCTCTCAAGTTCAATCCAGA+TGG - chr8.2:3660238-3660257 None:intergenic 45.0%
GACAGTCAATTCACCCAATG+AGG - chr8.2:3658818-3658837 None:intergenic 45.0%
GATAGAGAGAGGGTGATGAA+AGG + chr8.2:3659194-3659213 MS.gene042268:CDS 45.0%
GCATGAGAGGAGAATAGTAC+GGG - chr8.2:3658026-3658045 None:intergenic 45.0%
GGAAGAAGCCTCGTTTAGTT+TGG + chr8.2:3659498-3659517 MS.gene042268:CDS 45.0%
GGAGATTTGTGTACCTTACC+TGG + chr8.2:3660142-3660161 MS.gene042268:CDS 45.0%
GTGGGAACAATAGACCTCAT+TGG + chr8.2:3658801-3658820 MS.gene042268:intron 45.0%
GTGTGTGAGCTTACTGATGA+AGG - chr8.2:3659059-3659078 None:intergenic 45.0%
TAATGCAGGCACTAGTTCCA+TGG + chr8.2:3660900-3660919 MS.gene042268:CDS 45.0%
TGAGCTTACTGATGAAGGGT+AGG - chr8.2:3659054-3659073 None:intergenic 45.0%
TTCAAGCTCCTTGAGCAAGT+TGG + chr8.2:3659016-3659035 MS.gene042268:CDS 45.0%
TTGGGCTGACACATTAGTGT+TGG + chr8.2:3658776-3658795 MS.gene042268:intron 45.0%
TTGGGGTACATTATGGACCA+TGG - chr8.2:3660920-3660939 None:intergenic 45.0%
! AATGTGACATGTGTCGGTGT+TGG - chr8.2:3658226-3658245 None:intergenic 45.0%
! CTTCGGAGAAGATTTTCCTG+TGG + chr8.2:3657851-3657870 MS.gene042268:CDS 45.0%
ATAGCAGGACCTCCAACAGA+TGG - chr8.2:3660574-3660593 None:intergenic 50.0%
ATCTGTTGGAGGTCCTGCTA+TGG + chr8.2:3660573-3660592 MS.gene042268:CDS 50.0%
ATGATGAGGAACGCTCGACT+CGG + chr8.2:3659477-3659496 MS.gene042268:CDS 50.0%
CACCGGAACATTTATCCACC+TGG + chr8.2:3660391-3660410 MS.gene042268:CDS 50.0%
CAGTCCTTCTCCATTGAGTG+AGG + chr8.2:3660645-3660664 MS.gene042268:CDS 50.0%
CTCACTCAATGGAGAAGGAC+TGG - chr8.2:3660647-3660666 None:intergenic 50.0%
GAGTTTGAACCACGACGTCT+TGG + chr8.2:3658154-3658173 MS.gene042268:intron 50.0%
TACACGCAGCAGCAAAACGT+GGG + chr8.2:3660838-3660857 MS.gene042268:CDS 50.0%
TAGCAGGACCTCCAACAGAT+GGG - chr8.2:3660573-3660592 None:intergenic 50.0%
TCCTGTGGGAATGCATGTTC+TGG + chr8.2:3657866-3657885 MS.gene042268:CDS 50.0%
TTACACGCAGCAGCAAAACG+TGG + chr8.2:3660837-3660856 MS.gene042268:CDS 50.0%
!! AAAGGGTGTTCGAACGTCAG+TGG + chr8.2:3660354-3660373 MS.gene042268:CDS 50.0%
!! GGCTTTGGAGAATCCTTTGG+AGG + chr8.2:3657818-3657837 MS.gene042268:CDS 50.0%
GCCAGAACATGCATTCCCAC+AGG - chr8.2:3657870-3657889 None:intergenic 55.0%
! TGTCGGCTGAACGCACCATC+TGG + chr8.2:3660220-3660239 MS.gene042268:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 3657816 3661095 3657816 ID=MS.gene042268
chr8.2 mRNA 3657816 3661095 3657816 ID=MS.gene042268.t1;Parent=MS.gene042268
chr8.2 exon 3657816 3657954 3657816 ID=MS.gene042268.t1.exon1;Parent=MS.gene042268.t1
chr8.2 CDS 3657816 3657954 3657816 ID=cds.MS.gene042268.t1;Parent=MS.gene042268.t1
chr8.2 exon 3658912 3659064 3658912 ID=MS.gene042268.t1.exon2;Parent=MS.gene042268.t1
chr8.2 CDS 3658912 3659064 3658912 ID=cds.MS.gene042268.t1;Parent=MS.gene042268.t1
chr8.2 exon 3659177 3659329 3659177 ID=MS.gene042268.t1.exon3;Parent=MS.gene042268.t1
chr8.2 CDS 3659177 3659329 3659177 ID=cds.MS.gene042268.t1;Parent=MS.gene042268.t1
chr8.2 exon 3659360 3659575 3659360 ID=MS.gene042268.t1.exon4;Parent=MS.gene042268.t1
chr8.2 CDS 3659360 3659575 3659360 ID=cds.MS.gene042268.t1;Parent=MS.gene042268.t1
chr8.2 exon 3659726 3659802 3659726 ID=MS.gene042268.t1.exon5;Parent=MS.gene042268.t1
chr8.2 CDS 3659726 3659802 3659726 ID=cds.MS.gene042268.t1;Parent=MS.gene042268.t1
chr8.2 exon 3660049 3661095 3660049 ID=MS.gene042268.t1.exon6;Parent=MS.gene042268.t1
chr8.2 CDS 3660049 3661095 3660049 ID=cds.MS.gene042268.t1;Parent=MS.gene042268.t1
Gene Sequence

>MS.gene042268

ATGGCTTTGGAGAATCCTTTGGAGGTTGAAGATGTCTTCGGAGAAGATTTTCCTGTGGGAATGCATGTTCTGGCTGTTGACGACGATCAAACTTGTCTCAAACTGTTGGAAAAGTTTCTTCTCATGTGCAAGTATAATGGTTCGTTTCCATTTTTTCTTCTTTCTTTCAGTCAATTCATTTCTGCATTGATGATTCTTTGATGTTTTCCCGTACTATTCTCCTCTCATGCAGTGGTGTAAACTTATAATTGGAACTTTCATTGATATAGTAAACCCTTTTTTTAATCGGGGCAAATGTTACTTGTTAGTAATTTAGTGATGATTATATTAGTTTTTCAGAGTTTGAACCACGACGTCTTGGTTATAACTTATAAGCTTTGTAGTCGGTGCTGAGTGTGTGTTAGTATCCAACACCGACACATGTCACATTCAATCACTTGTTTTCTTAAAATATTTTCGATGTAATCTTGGCTACCCCAATTTTTTGTTTATTTCGGGTATAAATAAGTATACTTTTATGTTCAGCCACCCTAATAATATATGTTGACCTACCCCAAAGAAGTATATTTGCCAAAAAATTAGTAGAAACATAAAAGTTAAAATATTATTATTTAATTGATAATATTATTCGTCTTGTTTTGACATTTCTGGTCTTTACAACAAATATAGAGAGAACTTTGCTAGCAATGAAAATGGTAAGGTAAAAGTCGAAAATAAAAATAGAGGATGAGTTTCTTGCAAATAATTCAGTTGTTTTGATTGGAAAAGACACTGCTAGTCTGTTTGGTACAAATTATGAATGAATATGATTTTTTGAAGTGTTGCTGAATTCAACTTTCCTAAATACTGTTTTCTCATTTTCTATTATTCTGTGCTGCTCTAATTTACATTTAAAATAAGTAAAAATTATTGCAAATTATTGTAATGTAATTGTATATGTACATAAGATCACTACTTTTATTGGGCTGACACATTAGTGTTGGAAGTGGGAACAATAGACCTCATTGGGTGAATTGACTGTCCTTTATAACGCAGGAAGCCTTTTTATTTTAAATGTCTCTCACTGAATCACTACTTTGATGCTTGGTTGTTGCAGTTACTACAACTACGAAGTCAGTTGAAGCGCTAGAATTGTTGAGGGAAAAGAGAGAGATGTTTGACATTGTAATTAGTGATGTAAACATGCCAGAAATGGATGGATTCAAGCTCCTTGAGCAAGTTGGACATGAAATGGACCTACCCTTCATCAGTAAGCTCACACACCTTTGTTTTAGCATGTGTATTATTCAGATAACTAAGATTCACTTCTTATATTCATATGGTATCTTTAAGTGATGATTTCTTTTTGTGTGATCATATAGTGTTGTCAGGAAATGATGATAGAGAGAGGGTGATGAAAGGAGTGATGAAAGGCGCTTGCGACTATCTTGTGAAACCAATCCGATTGGAAGAGCTTAAGAACATATGGCAACATGTAGTGAGAAAGAAGATTGAAAGTAAGGATCCGAATAAAGGTATCATTACTGATGGGATATGTAATCAAGACACCTCATCTGAAAATATTGCTAACAAGAATAAAACCCGTGGACAAAAAATAAAGGAACAAAGTGAGGATGAAGAAGACGAAGAAGAAAACGCAGAAGAAGATAATGGTAGAGAGAATGATGAGGAACGCTCGACTCGGAAGAAGCCTCGTTTAGTTTGGGATGATGAATTGCATAAAAAATTTGTATCTATAGTTAGTCAACTCGGCCTTGATAGTGAGTTTATGTGACAATCATTTTTTTTTTGTTACTTTACTAATTTGATTGGCTTTAAAACATGGAACCTTAACAAGACCATTTGATGATTGAAACTTACATATGGATATTATTGTTCCTAACATGTACTTTCTTCAACTGTTATTGCAGAGGCTTATCCTAAGAAAATATGTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTGGCTAGCCATTTACAGGTACAAACTATAATTTCCCCTTTTGTCAAAATATTTCATACTAGTTTATCATTAAAATATTTGATCACCACATTTTTGCAAATATCGTCGAAGTTAAAGGGGATAATTTACTTTTAAACATATGCTTGTATGCAAATATGGTGTGAAAGTTTTTTATATCCATTATTCGTTGAAATTTCAACAGTCTCTTTTATGAAACATAAGTGGGAAAACTGCATGTAAGCTCGTTCTTGTTTTTTATTGCAGAAATACAAACTTAGTCTCAAAAGGCCATCTAAACAGGCTAGAGTAGATGCTGCATTAGATCCTCACCTACAAAAGAATTCAGTAGCTGGATATGGAGATTTGTGTACCTTACCTGGATCAAGAAGAATCTTAAGCAGCACATTGCCAACATATGCATCTAGTAACATGTATTGTCGGCTGAACGCACCATCTGGATTGAACTTGAGAGGAATGAGTTCTTCTGCATTAGTCCCTCCACTTCAGTCTCAAAACATTCCCAGTTACAAGCAGCCATTGTTTTTTGCAAGTGAAAACTCGAGTGCTTTAAAGGGTGTTCGAACGTCAGTGGAAATTAACCAGTTTCACCGGAACATTTATCCACCTGGTAACATGAACTTAAGTCCAATTGATGATTCAAGTGCATTTACAGATTCTTCTGGCTTCCAAGACATCAGAGCTAATGTTAATAATGCAAATAGTTTTCTTTCTTGCATCTCAAGCAATCACCTACAAACACATAATTCAAGAGCATTTATAAATCACCCATCTGTTGGAGGTCCTGCTATGGAACAAAAATCTTTTAACCCTGCTACAAGTGGTTCCTATAACTTTGCTTCCAGTCCTTCTCCATTGAGTGAGGATTTTAATAATGATCAAATATCTCACAATAGCTTAAAGTTTGCCTCGCTCAGTTCTCAATTTAGGAAAAGCCCAACTGATTTTTCTTCGACACGCGTCATTGATGTTCCTTTAGAGGAAACAATGCAATGTCAAGATGGATTACTTGGGAATGTTGTAGCGGCTTCATGTTACACGCAGCAGCAAAACGTGGGTTCTTCCTTCAACAATACCTTGGATTCTCTTGCTTCTTCTAATGCAGGCACTAGTTCCATGGTCCATAATGTACCCCAAGCTAATAGTCCATTAATCACTCAGATGCCTCAAGTTGAGAAATTTTATTCAGATGAAAGAACTATGGAATCAAATGGTGGTGATTGCTTTTTTGAGCAATTGCTTGATGTAGATGGATTCGTTCAGAATAGTTGTTTGCCTTGGATGATACAATAA
Protein sequence

>MS.gene042268.t1

MALENPLEVEDVFGEDFPVGMHVLAVDDDQTCLKLLEKFLLMCKYNVTTTTKSVEALELLREKREMFDIVISDVNMPEMDGFKLLEQVGHEMDLPFIMLSGNDDRERVMKGVMKGACDYLVKPIRLEELKNIWQHVVRKKIESKDPNKDTSSENIANKNKTRGQKIKEQSEDEEDEEENAEEDNGRENDEERSTRKKPRLVWDDELHKKFVSIVSQLGLDKAYPKKICDLMNVEGLTRENVASHLQKYKLSLKRPSKQARVDAALDPHLQKNSVAGYGDLCTLPGSRRILSSTLPTYASSNMYCRLNAPSGLNLRGMSSSALVPPLQSQNIPSYKQPLFFASENSSALKGVRTSVEINQFHRNIYPPGNMNLSPIDDSSAFTDSSGFQDIRANVNNANSFLSCISSNHLQTHNSRAFINHPSVGGPAMEQKSFNPATSGSYNFASSPSPLSEDFNNDQISHNSLKFASLSSQFRKSPTDFSSTRVIDVPLEETMQCQDGLLGNVVAASCYTQQQNVGSSFNNTLDSLASSNAGTSSMVHNVPQANSPLITQMPQVEKFYSDERTMESNGGDCFFEQLLDVDGFVQNSCLPWMIQ