AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042416

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042416.t1 MTR_7g074790 51.786 168 61 3 6 172 10 158 3.36e-46 160
MS.gene042416.t1 MTR_0402s0040 42.529 174 97 2 1 172 21 193 2.74e-38 146
MS.gene042416.t1 MTR_5g039760 42.778 180 69 5 8 178 12 166 2.15e-36 133
MS.gene042416.t1 MTR_4g102720 40.462 173 69 2 6 178 7 145 6.35e-35 130
MS.gene042416.t1 MTR_7g061980 52.252 111 51 2 6 115 10 119 1.93e-31 117
MS.gene042416.t1 MTR_6g051425 31.273 275 150 6 8 245 19 291 4.86e-31 123
MS.gene042416.t1 MTR_5g015480 44.094 127 69 1 1 125 51 177 4.64e-26 107
MS.gene042416.t1 MTR_0558s0020 29.070 258 131 6 10 216 40 296 2.92e-23 100
MS.gene042416.t1 MTR_2g070590 40.909 110 63 1 1 108 12 121 1.82e-21 90.1
MS.gene042416.t1 MTR_2g099430 54.118 85 39 0 31 115 28 112 1.97e-20 87.0
MS.gene042416.t1 MTR_6g017200 42.574 101 56 1 1 99 53 153 1.93e-19 85.5
MS.gene042416.t1 MTR_1g057920 46.667 90 46 1 6 93 34 123 2.02e-19 84.3
MS.gene042416.t1 MTR_1g070355 27.572 243 137 7 11 217 79 318 6.19e-19 90.1
MS.gene042416.t1 MTR_8g067580 64.615 65 7 1 63 127 3 51 2.20e-18 79.7
MS.gene042416.t1 MTR_1g086270 36.585 82 52 0 270 351 1 82 2.51e-18 80.9
MS.gene042416.t1 MTR_4g067290 43.137 102 56 2 6 106 56 156 1.40e-17 82.4
MS.gene042416.t1 MTR_4g067290 43.137 102 56 2 6 106 56 156 1.43e-17 82.4
MS.gene042416.t1 MTR_4g067290 43.137 102 56 2 6 106 76 176 2.64e-17 82.0
MS.gene042416.t1 MTR_4g067290 43.137 102 56 2 6 106 76 176 2.81e-17 82.0
MS.gene042416.t1 MTR_4g067290 43.137 102 56 2 6 106 74 174 2.93e-17 82.0
MS.gene042416.t1 MTR_4g067290 43.137 102 56 2 6 106 116 216 4.30e-17 82.0
MS.gene042416.t1 MTR_7g446520 28.194 227 118 6 10 206 92 303 6.96e-17 82.0
MS.gene042416.t1 MTR_7g113010 39.370 127 61 2 6 130 457 569 2.19e-16 82.4
MS.gene042416.t1 MTR_5g076070 26.496 234 139 5 3 204 50 282 4.26e-16 79.0
MS.gene042416.t1 MTR_4g076000 37.097 124 42 4 76 199 4 91 4.70e-16 74.3
MS.gene042416.t1 MTR_2g098920 41.176 102 58 2 6 106 80 180 1.99e-15 76.6
MS.gene042416.t1 MTR_1g008290 36.364 110 67 2 64 173 9 115 3.08e-15 73.2
MS.gene042416.t1 MTR_1g019850 33.582 134 80 2 6 137 70 196 8.97e-15 74.3
MS.gene042416.t1 MTR_7g446510 28.161 174 95 2 81 224 156 329 1.35e-14 75.1
MS.gene042416.t1 MTR_3g006370 36.719 128 79 2 6 131 50 177 3.07e-14 72.4
MS.gene042416.t1 MTR_2g098880 31.655 139 85 3 3 131 58 196 1.67e-13 70.9
MS.gene042416.t1 MTR_2g098880 31.655 139 85 3 3 131 70 208 1.73e-13 70.9
MS.gene042416.t1 MTR_6g022080 31.293 147 96 3 18 160 97 242 2.77e-13 70.1
MS.gene042416.t1 MTR_6g022070 33.043 115 70 3 270 383 1 109 3.62e-13 70.9
MS.gene042416.t1 MTR_7g446480 27.586 174 96 2 81 224 141 314 6.13e-13 70.1
MS.gene042416.t1 MTR_2g014100 28.415 183 111 5 10 172 48 230 1.74e-12 67.8
MS.gene042416.t1 MTR_1g083640 24.464 233 124 6 10 191 55 286 4.21e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042416.t1 AT4G38180 30.769 468 278 7 6 428 73 539 3.55e-74 249
MS.gene042416.t1 AT4G15090 31.463 410 248 4 6 383 51 459 2.00e-67 231
MS.gene042416.t1 AT4G15090 31.463 410 248 4 6 383 51 459 2.00e-67 231
MS.gene042416.t1 AT4G15090 31.463 410 248 4 6 383 51 459 2.00e-67 231
MS.gene042416.t1 AT4G15090 31.463 410 248 4 6 383 51 459 2.00e-67 231
MS.gene042416.t1 AT4G15090 31.463 410 248 4 6 383 51 459 2.00e-67 231
MS.gene042416.t1 AT3G22170 31.963 438 254 13 6 407 70 499 7.89e-65 224
MS.gene042416.t1 AT3G22170 31.963 438 254 13 6 407 70 499 7.89e-65 224
MS.gene042416.t1 AT1G76320 32.512 406 232 7 10 383 1 396 7.41e-57 200
MS.gene042416.t1 AT1G76320 32.512 406 232 7 10 383 1 396 1.08e-56 201
MS.gene042416.t1 AT1G76320 32.512 406 232 7 10 383 1 396 1.33e-56 201
MS.gene042416.t1 AT1G76320 32.512 406 232 7 10 383 1 396 1.33e-56 201
MS.gene042416.t1 AT3G06250 29.600 375 231 7 4 367 188 540 7.01e-52 188
MS.gene042416.t1 AT3G06250 36.275 102 51 2 6 106 28 116 1.46e-12 70.5
MS.gene042416.t1 AT3G06250 29.600 375 231 7 4 367 188 540 7.01e-52 188
MS.gene042416.t1 AT3G06250 36.275 102 51 2 6 106 28 116 1.46e-12 70.5
MS.gene042416.t1 AT3G06250 29.600 375 231 7 4 367 188 540 7.01e-52 188
MS.gene042416.t1 AT3G06250 36.275 102 51 2 6 106 28 116 1.46e-12 70.5
MS.gene042416.t1 AT5G18960 28.608 388 227 9 1 367 206 564 1.50e-49 181
MS.gene042416.t1 AT5G18960 36.275 102 51 2 6 106 43 131 2.11e-12 70.1
MS.gene042416.t1 AT5G18960 28.608 388 227 9 1 367 206 564 1.50e-49 181
MS.gene042416.t1 AT5G18960 36.275 102 51 2 6 106 43 131 2.11e-12 70.1
MS.gene042416.t1 AT2G27110 30.130 385 235 9 6 371 49 418 1.81e-48 179
MS.gene042416.t1 AT2G27110 30.130 385 235 9 6 371 49 418 1.81e-48 179
MS.gene042416.t1 AT2G32250 28.042 378 254 4 9 383 41 403 8.28e-48 176
MS.gene042416.t1 AT2G32250 28.042 378 254 4 9 383 41 403 8.41e-48 177
MS.gene042416.t1 AT2G32250 28.042 378 254 4 9 383 41 403 8.41e-48 177
MS.gene042416.t1 AT2G32250 28.042 378 254 4 9 383 41 403 9.85e-48 176
MS.gene042416.t1 AT2G32250 28.042 378 254 4 9 383 41 403 9.85e-48 176
MS.gene042416.t1 AT2G32250 28.042 378 254 4 9 383 41 403 9.85e-48 176
MS.gene042416.t1 AT4G19990 27.665 394 256 7 9 383 24 407 2.04e-47 175
MS.gene042416.t1 AT4G19990 27.665 394 256 7 9 383 24 407 2.04e-47 175
MS.gene042416.t1 AT4G19990 27.665 394 256 7 9 383 24 407 2.28e-47 175
MS.gene042416.t1 AT4G19990 27.665 394 256 7 9 383 38 421 2.40e-47 175
MS.gene042416.t1 AT4G19990 27.665 394 256 7 9 383 24 407 2.49e-47 175
MS.gene042416.t1 AT4G19990 27.665 394 256 7 9 383 24 407 2.49e-47 175
MS.gene042416.t1 AT2G32250 27.851 377 254 4 10 383 1 362 3.93e-47 174
MS.gene042416.t1 AT2G32250 27.851 377 254 4 10 383 1 362 4.18e-47 174
MS.gene042416.t1 AT1G52520 29.484 407 246 13 6 383 85 479 4.77e-42 159
MS.gene042416.t1 AT4G19990 28.614 332 222 6 57 383 29 350 1.12e-41 158
MS.gene042416.t1 AT1G80010 26.355 406 253 8 6 372 95 493 3.00e-41 157
MS.gene042416.t1 AT1G10240 24.574 411 272 7 5 378 47 456 1.68e-39 152
MS.gene042416.t1 AT1G10240 24.574 411 272 7 5 378 47 456 1.68e-39 152
MS.gene042416.t1 AT2G27110 33.000 200 134 0 172 371 74 273 5.23e-39 150
MS.gene042416.t1 AT5G28530 23.776 429 273 10 2 381 51 474 4.43e-33 133
MS.gene042416.t1 AT5G28530 23.776 429 273 10 2 381 51 474 4.43e-33 133
MS.gene042416.t1 AT5G28530 23.776 429 273 10 2 381 51 474 4.80e-33 133
MS.gene042416.t1 AT4G38170 29.515 227 151 3 165 383 23 248 5.91e-33 132
MS.gene042416.t1 AT5G28530 24.103 390 257 11 2 381 51 411 5.12e-26 112
MS.gene042416.t1 AT5G28530 24.103 390 257 11 2 381 51 411 5.53e-26 112
MS.gene042416.t1 AT3G59470 43.137 102 56 2 6 106 70 170 1.12e-17 83.2
MS.gene042416.t1 AT3G59470 43.137 102 56 2 6 106 70 170 1.12e-17 83.2
MS.gene042416.t1 AT3G59470 43.137 102 56 2 6 106 82 182 1.39e-17 83.2
MS.gene042416.t1 AT3G07500 38.136 118 61 2 6 111 33 150 2.07e-13 69.7
MS.gene042416.t1 AT2G43280 34.375 128 82 2 6 131 22 149 7.03e-13 68.2
MS.gene042416.t1 AT4G12850 36.290 124 73 3 6 126 11 131 1.50e-12 65.5
MS.gene042416.t1 AT4G12850 38.462 104 60 2 6 108 11 111 1.51e-12 65.5
MS.gene042416.t1 AT4G12850 35.772 123 72 3 6 124 11 130 7.23e-12 64.7

Find 85 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAATCACAAATGTATTT+TGG 0.093989 6.2:+81358753 MS.gene042416:CDS
ATCTTAGATTATGGATATTT+TGG 0.112546 6.2:+81358791 MS.gene042416:CDS
TAGTTTCACTAGATTATTAA+TGG 0.236771 6.2:+81360299 MS.gene042416:CDS
TAGATGAAGCTTGGCAGTTT+TGG 0.257858 6.2:+81358178 MS.gene042416:CDS
CTTGATCTGTGAACAAAGTT+TGG 0.328870 6.2:-81359000 None:intergenic
TTGATCTGTGAACAAAGTTT+GGG 0.334950 6.2:-81358999 None:intergenic
CCCTTTGCTGTTGTCTCAAT+CGG 0.342411 6.2:-81360002 None:intergenic
GACAAAAGCGATTCAAAAGT+TGG 0.351490 6.2:+81360051 MS.gene042416:CDS
AAATGACAATGCCTCTATAA+TGG 0.355327 6.2:-81358889 None:intergenic
GAAGTCGATGTATGCTATTA+AGG 0.356692 6.2:+81359255 MS.gene042416:CDS
GTTGAGTATGGTAAAGAAAC+TGG 0.361914 6.2:+81358201 MS.gene042416:CDS
AACAAACCTGCATGATCTAA+TGG 0.380514 6.2:-81358271 None:intergenic
TTAATGTCAATGAAAACAAT+TGG 0.384682 6.2:+81359230 MS.gene042416:CDS
TCAGTCCTTGTTTCAGCTCT+AGG 0.400631 6.2:-81358348 None:intergenic
AACTGGCTTTGGTGTTAGAA+AGG 0.408770 6.2:+81358218 MS.gene042416:CDS
CAACGGTCTATTAGCATGAT+TGG 0.420431 6.2:-81358846 None:intergenic
CATAACCCATGTCGAGTCTC+TGG 0.431181 6.2:-81359058 None:intergenic
ATCTTTCAAATGGTTATTTG+AGG 0.432948 6.2:+81358946 MS.gene042416:CDS
ATTGCACTTAGTTCAAAGAA+TGG 0.438544 6.2:+81358384 MS.gene042416:CDS
CTTCGTCTTGTCATCAATCA+AGG 0.445012 6.2:+81360111 MS.gene042416:CDS
CTAGTGCTTTAGACATTGCC+TGG 0.451171 6.2:-81359024 None:intergenic
AGGAGAAGTTTGAGACAGCT+TGG 0.459277 6.2:+81359188 MS.gene042416:CDS
GGACTCTGGTTTGAGGCAAA+AGG 0.461228 6.2:+81358533 MS.gene042416:CDS
TCAGGCTTTAACCATTATAG+AGG 0.461587 6.2:+81358878 MS.gene042416:CDS
GCAGGTGGTAGAACTAATCT+TGG 0.464714 6.2:+81358582 MS.gene042416:CDS
CCGATTGAGACAACAGCAAA+GGG 0.468981 6.2:+81360002 MS.gene042416:CDS
CAGATCGTCTTTCAGACATC+AGG 0.469794 6.2:+81360234 MS.gene042416:intron
TAATGTCAATGAAAACAATT+GGG 0.472275 6.2:+81359231 MS.gene042416:CDS
AAACTGCTGAATCTTTCAAA+TGG 0.482492 6.2:+81358936 MS.gene042416:CDS
ATTGAAATGGCAGAGGACTC+TGG 0.482937 6.2:+81358519 MS.gene042416:CDS
ACTAAATAAGATCTCTTGTC+CGG 0.485435 6.2:-81358318 None:intergenic
CAAGACGAAGGTTGTACAAT+CGG 0.487830 6.2:-81360098 None:intergenic
CAGCAAAGGGGTATAAGAAT+CGG 0.494285 6.2:+81360015 MS.gene042416:CDS
TCAAGCATATGAGATTGAAA+TGG 0.499950 6.2:+81358506 MS.gene042416:CDS
TGGCAGAGGACTCTGGTTTG+AGG 0.506073 6.2:+81358526 MS.gene042416:CDS
GAAGCTGTAAATCGTGGTTA+TGG 0.510351 6.2:-81358437 None:intergenic
CATGCCAACACTAGGTATAG+TGG 0.514325 6.2:-81358142 None:intergenic
TTGATAGTGTAGATGAAGCT+TGG 0.514856 6.2:+81358169 MS.gene042416:CDS
CTCGACATGGGTTATGTACA+TGG 0.515437 6.2:+81359065 MS.gene042416:CDS
AGGTTTGTTTGTTGCAAGGA+GGG 0.516993 6.2:+81358285 MS.gene042416:CDS
CAGGTTTGTTTGTTGCAAGG+AGG 0.519394 6.2:+81358284 MS.gene042416:CDS
TATGGTCCTTCACAAACTCA+TGG 0.520238 6.2:-81358419 None:intergenic
CACTATACCTAGTGTTGGCA+TGG 0.524699 6.2:+81358143 MS.gene042416:CDS
ATGCAGGTTTGTTTGTTGCA+AGG 0.530015 6.2:+81358281 MS.gene042416:CDS
CAAGGAGGGTCATCGGATAC+CGG 0.531140 6.2:+81358299 MS.gene042416:CDS
GTTTGAGATAACTCATGTGT+TGG 0.539450 6.2:-81360267 None:intergenic
TTTGTTGCAAGGAGGGTCAT+CGG 0.544826 6.2:+81358292 MS.gene042416:CDS
TATGGTAAAGAAACTGGCTT+TGG 0.553868 6.2:+81358207 MS.gene042416:CDS
CATGTATGATTATGATCAAG+AGG 0.554480 6.2:+81359168 MS.gene042416:CDS
ATGAAGGAAGCATTCACTCT+AGG 0.565288 6.2:+81359298 MS.gene042416:CDS
AATGCAACGGTCAATGATGA+AGG 0.565367 6.2:+81359957 MS.gene042416:CDS
TGAGACAGCTTGGAGCAAGT+TGG 0.566720 6.2:+81359198 MS.gene042416:CDS
TGTAATCCATGAGTTTGTGA+AGG 0.578123 6.2:+81358413 MS.gene042416:CDS
TAATCTTGGATATACACGGT+TGG 0.580985 6.2:+81358596 MS.gene042416:CDS
CAACTTATGAGTGCACAAGC+AGG 0.586209 6.2:+81358564 MS.gene042416:CDS
ACAGCTCCATTAGATCATGC+AGG 0.587384 6.2:+81358265 MS.gene042416:CDS
TCAAACTCCATGCCAACACT+AGG 0.589517 6.2:-81358150 None:intergenic
ATGGGCAGCATGTCACATGA+AGG 0.590749 6.2:+81359282 MS.gene042416:CDS
CAGAATGGTATAAAACACCT+TGG 0.591052 6.2:+81359097 MS.gene042416:CDS
TGAAGGTGGTGTTTCGACAT+TGG 0.593814 6.2:+81359974 MS.gene042416:CDS
GCTAGCATGTGTGTTGTCTG+TGG 0.599379 6.2:-81358459 None:intergenic
CAATCATGCTAATAGACCGT+TGG 0.608792 6.2:+81358847 MS.gene042416:CDS
ATATGAGATTGAAATGGCAG+AGG 0.610938 6.2:+81358512 MS.gene042416:CDS
ACAACCACTATACCTAGTGT+TGG 0.612031 6.2:+81358138 MS.gene042416:CDS
TTCGTCTTGTCATCAATCAA+GGG 0.613908 6.2:+81360112 MS.gene042416:CDS
ACCGATTGAGACAACAGCAA+AGG 0.615299 6.2:+81360001 MS.gene042416:CDS
TGATCTGTGAACAAAGTTTG+GGG 0.616827 6.2:-81358998 None:intergenic
GAGTCAATTGAACATGAATG+AGG 0.618296 6.2:+81360404 MS.gene042416:CDS
GAGGTATTTCTTGAAGCACA+TGG 0.618467 6.2:+81358965 MS.gene042416:CDS
AGAATCCTAGAGCTGAAACA+AGG 0.627112 6.2:+81358343 MS.gene042416:CDS
AAGGGGTATAAGAATCGGCG+TGG 0.638130 6.2:+81360020 MS.gene042416:CDS
GTGATGCCAGAGACTCGACA+TGG 0.639006 6.2:+81359052 MS.gene042416:CDS
ACATGGCACTTGATGCAGAA+TGG 0.650022 6.2:+81359082 MS.gene042416:CDS
AAGCCTGAAAACAATGCCAA+CGG 0.656439 6.2:-81358863 None:intergenic
CGTCTTTCAGACATCAGGTG+TGG 0.666655 6.2:+81360239 MS.gene042416:intron
CTTATGAGTGCACAAGCAGG+TGG 0.669554 6.2:+81358567 MS.gene042416:CDS
AGGGGTATAAGAATCGGCGT+GGG 0.674899 6.2:+81360021 MS.gene042416:CDS
TAGACTAGAACAAAATGCAA+CGG 0.678964 6.2:+81359944 MS.gene042416:CDS
CTTGATTGATGACAAGACGA+AGG 0.695787 6.2:-81360110 None:intergenic
GAACTAATCTTGGATATACA+CGG 0.698243 6.2:+81358592 MS.gene042416:CDS
TTTGTTCACAGATCAAGACC+AGG 0.702976 6.2:+81359006 MS.gene042416:CDS
GCAACGGTCAATGATGAAGG+TGG 0.714492 6.2:+81359960 MS.gene042416:CDS
TGATGCCAGAGACTCGACAT+GGG 0.714570 6.2:+81359053 MS.gene042416:CDS
CGATTGAGACAACAGCAAAG+GGG 0.730503 6.2:+81360003 MS.gene042416:CDS
TCTGTGGAAGCTGTAAATCG+TGG 0.747227 6.2:-81358443 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTTAAAATCATTGA+AGG + chr6.2:81360197-81360216 MS.gene042416:intron 10.0%
!! AAAATAAGGAAAATTCATTA+TGG - chr6.2:81359561-81359580 None:intergenic 15.0%
!! AAATCAATAACATTGATGTT+TGG - chr6.2:81359382-81359401 None:intergenic 20.0%
!! ATTATGGAATTTGTCAAAAA+TGG - chr6.2:81359545-81359564 None:intergenic 20.0%
!! TAATGTCAATGAAAACAATT+GGG + chr6.2:81359231-81359250 MS.gene042416:CDS 20.0%
!! TACACAATATCATAAAAAGT+TGG - chr6.2:81360368-81360387 None:intergenic 20.0%
!! TAGTTTCACTAGATTATTAA+TGG + chr6.2:81360299-81360318 MS.gene042416:CDS 20.0%
!! TTAATGTCAATGAAAACAAT+TGG + chr6.2:81359230-81359249 MS.gene042416:CDS 20.0%
!!! AACAAATCACAAATGTATTT+TGG + chr6.2:81358753-81358772 MS.gene042416:CDS 20.0%
!!! ACAAATCACAAATGTATTTT+GGG + chr6.2:81358754-81358773 MS.gene042416:CDS 20.0%
!!! ATCTTAGATTATGGATATTT+TGG + chr6.2:81358791-81358810 MS.gene042416:CDS 20.0%
!!! TTTTTAAAATCATTGAAGGT+TGG + chr6.2:81360201-81360220 MS.gene042416:intron 20.0%
! ATCTTTCAAATGGTTATTTG+AGG + chr6.2:81358946-81358965 MS.gene042416:CDS 25.0%
! GCAAAAATGATCTTAGATTA+TGG + chr6.2:81358782-81358801 MS.gene042416:CDS 25.0%
! GTATGCTATTAAGGAAAAAT+GGG + chr6.2:81359264-81359283 MS.gene042416:CDS 25.0%
! TGATGTGCATAAAAAAATGA+AGG - chr6.2:81358719-81358738 None:intergenic 25.0%
! TGTATGCTATTAAGGAAAAA+TGG + chr6.2:81359263-81359282 MS.gene042416:CDS 25.0%
! TTATTAATGGTAAGATTCCT+TGG + chr6.2:81360312-81360331 MS.gene042416:intron 25.0%
! TTGATCAACAATGATTAGAT+TGG + chr6.2:81359839-81359858 MS.gene042416:intron 25.0%
!! CTTGAACTTCAGATATTTTA+CGG - chr6.2:81358491-81358510 None:intergenic 25.0%
!! GAGTTTGAATTCTGATTTTA+AGG + chr6.2:81359345-81359364 MS.gene042416:CDS 25.0%
!!! ATTTTTTTATGCACATCAGA+TGG + chr6.2:81358721-81358740 MS.gene042416:intron 25.0%
!!! TGTGTATGTGTTTTTAATGT+AGG + chr6.2:81360382-81360401 MS.gene042416:intron 25.0%
!!! TTTTTTTATGCACATCAGAT+GGG + chr6.2:81358722-81358741 MS.gene042416:intron 25.0%
AAACTGCTGAATCTTTCAAA+TGG + chr6.2:81358936-81358955 MS.gene042416:CDS 30.0%
AAATGACAATGCCTCTATAA+TGG - chr6.2:81358892-81358911 None:intergenic 30.0%
AAGACAAGTAACATGGTTTA+TGG + chr6.2:81358644-81358663 MS.gene042416:intron 30.0%
AAGGTGATGACATATTCAAA+TGG - chr6.2:81359621-81359640 None:intergenic 30.0%
ACTAAATAAGATCTCTTGTC+CGG - chr6.2:81358321-81358340 None:intergenic 30.0%
AGACAAGTAACATGGTTTAT+GGG + chr6.2:81358645-81358664 MS.gene042416:intron 30.0%
ATTGCACTTAGTTCAAAGAA+TGG + chr6.2:81358384-81358403 MS.gene042416:CDS 30.0%
ATTTGAATATGTCATCACCT+TGG + chr6.2:81359620-81359639 MS.gene042416:intron 30.0%
CATGTATGATTATGATCAAG+AGG + chr6.2:81359168-81359187 MS.gene042416:CDS 30.0%
CCTTGGTCTATAAAAAGTAA+TGG + chr6.2:81360329-81360348 MS.gene042416:intron 30.0%
CGTTGCATCATGTATAATTT+GGG - chr6.2:81359807-81359826 None:intergenic 30.0%
GAAAAAAGGAAGTCATTTCT+TGG + chr6.2:81359129-81359148 MS.gene042416:CDS 30.0%
GAACTAATCTTGGATATACA+CGG + chr6.2:81358592-81358611 MS.gene042416:CDS 30.0%
GACATATTCAAATGGAAGTT+GGG - chr6.2:81359613-81359632 None:intergenic 30.0%
TAGACTAGAACAAAATGCAA+CGG + chr6.2:81359944-81359963 MS.gene042416:CDS 30.0%
TATACATGATGCAACGATAT+AGG + chr6.2:81359811-81359830 MS.gene042416:intron 30.0%
TCAAGCATATGAGATTGAAA+TGG + chr6.2:81358506-81358525 MS.gene042416:CDS 30.0%
TCGTTGCATCATGTATAATT+TGG - chr6.2:81359808-81359827 None:intergenic 30.0%
TCTGCAAGTTCATATAATGT+AGG - chr6.2:81359885-81359904 None:intergenic 30.0%
TGACATATTCAAATGGAAGT+TGG - chr6.2:81359614-81359633 None:intergenic 30.0%
TTCATATAATGTAGGAGATG+TGG - chr6.2:81359877-81359896 None:intergenic 30.0%
TTGATCTGTGAACAAAGTTT+GGG - chr6.2:81359002-81359021 None:intergenic 30.0%
! CTTGGTAACTTGATGAAAAA+AGG + chr6.2:81359115-81359134 MS.gene042416:CDS 30.0%
! TGACTTGTGTAAAAAAGTGA+TGG + chr6.2:81359917-81359936 MS.gene042416:CDS 30.0%
!! TGTAGAAAGTTTGAGACTTT+TGG + chr6.2:81359720-81359739 MS.gene042416:intron 30.0%
!!! AGATTATGGATATTTTGGTG+AGG + chr6.2:81358796-81358815 MS.gene042416:CDS 30.0%
!!! ATAGAGGCATTGTCATTTTT+GGG + chr6.2:81358894-81358913 MS.gene042416:CDS 30.0%
!!! CCATTACTTTTTATAGACCA+AGG - chr6.2:81360332-81360351 None:intergenic 30.0%
!!! CTTTTTTCATCAAGTTACCA+AGG - chr6.2:81359117-81359136 None:intergenic 30.0%
!!! TATAGAGGCATTGTCATTTT+TGG + chr6.2:81358893-81358912 MS.gene042416:CDS 30.0%
AACAAACCTGCATGATCTAA+TGG - chr6.2:81358274-81358293 None:intergenic 35.0%
AAGCAAGAAGACAAGTAACA+TGG + chr6.2:81358637-81358656 MS.gene042416:intron 35.0%
ATATGAGATTGAAATGGCAG+AGG + chr6.2:81358512-81358531 MS.gene042416:CDS 35.0%
CAATAAGGAAGGAGAGTTTA+AGG + chr6.2:81359644-81359663 MS.gene042416:intron 35.0%
CAGAATGGTATAAAACACCT+TGG + chr6.2:81359097-81359116 MS.gene042416:CDS 35.0%
CATGATATAGATGATTGTGC+AGG - chr6.2:81359696-81359715 None:intergenic 35.0%
CTTGATCTGTGAACAAAGTT+TGG - chr6.2:81359003-81359022 None:intergenic 35.0%
CTTGTGTAAAAAAGTGATGG+AGG + chr6.2:81359920-81359939 MS.gene042416:CDS 35.0%
GAAGTCGATGTATGCTATTA+AGG + chr6.2:81359255-81359274 MS.gene042416:CDS 35.0%
GAATGGCTGTATATCCAATT+TGG - chr6.2:81359484-81359503 None:intergenic 35.0%
GACAAAAGCGATTCAAAAGT+TGG + chr6.2:81360051-81360070 MS.gene042416:CDS 35.0%
GACAAGTAACATGGTTTATG+GGG + chr6.2:81358646-81358665 MS.gene042416:intron 35.0%
GTTGAGTATGGTAAAGAAAC+TGG + chr6.2:81358201-81358220 MS.gene042416:CDS 35.0%
GTTGCACTAAAATTGGTGAA+TGG - chr6.2:81359501-81359520 None:intergenic 35.0%
TAATCTTGGATATACACGGT+TGG + chr6.2:81358596-81358615 MS.gene042416:CDS 35.0%
TATGCGAGCAGCTAAAAATA+AGG - chr6.2:81359575-81359594 None:intergenic 35.0%
TGATCTGTGAACAAAGTTTG+GGG - chr6.2:81359001-81359020 None:intergenic 35.0%
TGGAATTTGTCAAAAATGGC+AGG - chr6.2:81359541-81359560 None:intergenic 35.0%
TGTAATCCATGAGTTTGTGA+AGG + chr6.2:81358413-81358432 MS.gene042416:CDS 35.0%
TTCGTCTTGTCATCAATCAA+GGG + chr6.2:81360112-81360131 MS.gene042416:CDS 35.0%
! GTTTGAGATAACTCATGTGT+TGG - chr6.2:81360270-81360289 None:intergenic 35.0%
! TATGGTAAAGAAACTGGCTT+TGG + chr6.2:81358207-81358226 MS.gene042416:CDS 35.0%
! TCAGGCTTTAACCATTATAG+AGG + chr6.2:81358878-81358897 MS.gene042416:CDS 35.0%
!! CGAGTTAGTTTGTGAATATG+AGG + chr6.2:81359444-81359463 MS.gene042416:intron 35.0%
!! TTGATAGTGTAGATGAAGCT+TGG + chr6.2:81358169-81358188 MS.gene042416:CDS 35.0%
!!! TAGAGGCATTGTCATTTTTG+GGG + chr6.2:81358895-81358914 MS.gene042416:CDS 35.0%
!!! TTATGGATATTTTGGTGAGG+TGG + chr6.2:81358799-81358818 MS.gene042416:CDS 35.0%
AACATGGTTTATGGGGAAGT+TGG + chr6.2:81358653-81358672 MS.gene042416:intron 40.0%
AAGCCTGAAAACAATGCCAA+CGG - chr6.2:81358866-81358885 None:intergenic 40.0%
AAGGAAGGAGAGTTTAAGGA+AGG + chr6.2:81359648-81359667 MS.gene042416:intron 40.0%
AATGCAACGGTCAATGATGA+AGG + chr6.2:81359957-81359976 MS.gene042416:CDS 40.0%
ACAACCACTATACCTAGTGT+TGG + chr6.2:81358138-81358157 MS.gene042416:CDS 40.0%
AGAATCCTAGAGCTGAAACA+AGG + chr6.2:81358343-81358362 MS.gene042416:CDS 40.0%
ATCACCTTGGTCAATAAGGA+AGG + chr6.2:81359633-81359652 MS.gene042416:intron 40.0%
CAAACACATTGAGAGAGTTG+TGG + chr6.2:81359405-81359424 MS.gene042416:intron 40.0%
CAACGGTCTATTAGCATGAT+TGG - chr6.2:81358849-81358868 None:intergenic 40.0%
CAAGACGAAGGTTGTACAAT+CGG - chr6.2:81360101-81360120 None:intergenic 40.0%
CAATCATGCTAATAGACCGT+TGG + chr6.2:81358847-81358866 MS.gene042416:CDS 40.0%
CTCGACATAAACTGCCAAAT+TGG + chr6.2:81359467-81359486 MS.gene042416:intron 40.0%
CTTCGTCTTGTCATCAATCA+AGG + chr6.2:81360111-81360130 MS.gene042416:CDS 40.0%
GAAGCTGTAAATCGTGGTTA+TGG - chr6.2:81358440-81358459 None:intergenic 40.0%
TATGGTCCTTCACAAACTCA+TGG - chr6.2:81358422-81358441 None:intergenic 40.0%
TGTAGGAGATGTGGATACAT+CGG - chr6.2:81359868-81359887 None:intergenic 40.0%
TGTCATCACCTTGGTCAATA+AGG + chr6.2:81359629-81359648 MS.gene042416:intron 40.0%
TTTGTTCACAGATCAAGACC+AGG + chr6.2:81359006-81359025 MS.gene042416:CDS 40.0%
! AGATGAAGCTTGGCAGTTTT+GGG + chr6.2:81358179-81358198 MS.gene042416:CDS 40.0%
! AGGTTTGTTTGTTGCAAGGA+GGG + chr6.2:81358285-81358304 MS.gene042416:CDS 40.0%
! ATGCAGGTTTGTTTGTTGCA+AGG + chr6.2:81358281-81358300 MS.gene042416:CDS 40.0%
! CAGCAAAGGGGTATAAGAAT+CGG + chr6.2:81360015-81360034 MS.gene042416:CDS 40.0%
! CTTGATTGATGACAAGACGA+AGG - chr6.2:81360113-81360132 None:intergenic 40.0%
! GAGGTATTTCTTGAAGCACA+TGG + chr6.2:81358965-81358984 MS.gene042416:CDS 40.0%
! TAGATGAAGCTTGGCAGTTT+TGG + chr6.2:81358178-81358197 MS.gene042416:CDS 40.0%
! TGTACAATCGGAGCTCTTTT+AGG - chr6.2:81360089-81360108 None:intergenic 40.0%
!! AACTGGCTTTGGTGTTAGAA+AGG + chr6.2:81358218-81358237 MS.gene042416:CDS 40.0%
!! ACTCAAGATCTTTGAGGCAA+AGG + chr6.2:81359761-81359780 MS.gene042416:intron 40.0%
!! ATGAAGGAAGCATTCACTCT+AGG + chr6.2:81359298-81359317 MS.gene042416:CDS 40.0%
!! CTTTGAGGCAAAGGAAAATG+AGG + chr6.2:81359770-81359789 MS.gene042416:intron 40.0%
ACACATTGAGAGAGTTGTGG+AGG + chr6.2:81359408-81359427 MS.gene042416:intron 45.0%
ACAGCTCCATTAGATCATGC+AGG + chr6.2:81358265-81358284 MS.gene042416:CDS 45.0%
ACATGGCACTTGATGCAGAA+TGG + chr6.2:81359082-81359101 MS.gene042416:CDS 45.0%
ACCGATTGAGACAACAGCAA+AGG + chr6.2:81360001-81360020 MS.gene042416:CDS 45.0%
ATTGAAATGGCAGAGGACTC+TGG + chr6.2:81358519-81358538 MS.gene042416:CDS 45.0%
CAACTTATGAGTGCACAAGC+AGG + chr6.2:81358564-81358583 MS.gene042416:CDS 45.0%
CAGATCGTCTTTCAGACATC+AGG + chr6.2:81360234-81360253 MS.gene042416:intron 45.0%
CATGCCAACACTAGGTATAG+TGG - chr6.2:81358145-81358164 None:intergenic 45.0%
CCATGCACTCAAGATCTTTG+AGG + chr6.2:81359755-81359774 MS.gene042416:intron 45.0%
CCGATTGAGACAACAGCAAA+GGG + chr6.2:81360002-81360021 MS.gene042416:CDS 45.0%
CCTCAAAGATCTTGAGTGCA+TGG - chr6.2:81359758-81359777 None:intergenic 45.0%
CGATTGAGACAACAGCAAAG+GGG + chr6.2:81360003-81360022 MS.gene042416:CDS 45.0%
CTCTCCTTCCTTATTGACCA+AGG - chr6.2:81359640-81359659 None:intergenic 45.0%
GAGAGTTTAAGGAAGGAGAG+TGG + chr6.2:81359655-81359674 MS.gene042416:intron 45.0%
GCAGGTGGTAGAACTAATCT+TGG + chr6.2:81358582-81358601 MS.gene042416:CDS 45.0%
GGCAAAGGAAAATGAGGTTG+TGG + chr6.2:81359776-81359795 MS.gene042416:intron 45.0%
TCAAACTCCATGCCAACACT+AGG - chr6.2:81358153-81358172 None:intergenic 45.0%
TCTGTGGAAGCTGTAAATCG+TGG - chr6.2:81358446-81358465 None:intergenic 45.0%
TGAAGGTGGTGTTTCGACAT+TGG + chr6.2:81359974-81359993 MS.gene042416:CDS 45.0%
TGCGCGAGTTGCACTAAAAT+TGG - chr6.2:81359508-81359527 None:intergenic 45.0%
TTTGTTGCAAGGAGGGTCAT+CGG + chr6.2:81358292-81358311 MS.gene042416:CDS 45.0%
! AGACCGTTGGCATTGTTTTC+AGG + chr6.2:81358860-81358879 MS.gene042416:CDS 45.0%
! AGGAGAAGTTTGAGACAGCT+TGG + chr6.2:81359188-81359207 MS.gene042416:CDS 45.0%
! CAGGTTTGTTTGTTGCAAGG+AGG + chr6.2:81358284-81358303 MS.gene042416:CDS 45.0%
! CTAGTGCTTTAGACATTGCC+TGG - chr6.2:81359027-81359046 None:intergenic 45.0%
! CTCGACATGGGTTATGTACA+TGG + chr6.2:81359065-81359084 MS.gene042416:CDS 45.0%
! TCAGTCCTTGTTTCAGCTCT+AGG - chr6.2:81358351-81358370 None:intergenic 45.0%
!! CACTATACCTAGTGTTGGCA+TGG + chr6.2:81358143-81358162 MS.gene042416:CDS 45.0%
!! CCCTTTGCTGTTGTCTCAAT+CGG - chr6.2:81360005-81360024 None:intergenic 45.0%
!!! TGGCAGTTTTGGGTTGAGTA+TGG + chr6.2:81358189-81358208 MS.gene042416:CDS 45.0%
ATGGGCAGCATGTCACATGA+AGG + chr6.2:81359282-81359301 MS.gene042416:CDS 50.0%
CATAACCCATGTCGAGTCTC+TGG - chr6.2:81359061-81359080 None:intergenic 50.0%
CGTCTTTCAGACATCAGGTG+TGG + chr6.2:81360239-81360258 MS.gene042416:intron 50.0%
CTTATGAGTGCACAAGCAGG+TGG + chr6.2:81358567-81358586 MS.gene042416:CDS 50.0%
GCAACGGTCAATGATGAAGG+TGG + chr6.2:81359960-81359979 MS.gene042416:CDS 50.0%
GCTAGCATGTGTGTTGTCTG+TGG - chr6.2:81358462-81358481 None:intergenic 50.0%
TGAGACAGCTTGGAGCAAGT+TGG + chr6.2:81359198-81359217 MS.gene042416:CDS 50.0%
TGATGCCAGAGACTCGACAT+GGG + chr6.2:81359053-81359072 MS.gene042416:CDS 50.0%
! AAGGGGTATAAGAATCGGCG+TGG + chr6.2:81360020-81360039 MS.gene042416:CDS 50.0%
! AGGGGTATAAGAATCGGCGT+GGG + chr6.2:81360021-81360040 MS.gene042416:CDS 50.0%
! GGACTCTGGTTTGAGGCAAA+AGG + chr6.2:81358533-81358552 MS.gene042416:CDS 50.0%
CAAGGAGGGTCATCGGATAC+CGG + chr6.2:81358299-81358318 MS.gene042416:CDS 55.0%
GTGATGCCAGAGACTCGACA+TGG + chr6.2:81359052-81359071 MS.gene042416:CDS 55.0%
! TGGCAGAGGACTCTGGTTTG+AGG + chr6.2:81358526-81358545 MS.gene042416:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 81358135 81360424 81358135 ID=MS.gene042416
chr6.2 mRNA 81358135 81360424 81358135 ID=MS.gene042416.t1;Parent=MS.gene042416
chr6.2 exon 81358135 81358650 81358135 ID=MS.gene042416.t1.exon1;Parent=MS.gene042416.t1
chr6.2 CDS 81358135 81358650 81358135 ID=cds.MS.gene042416.t1;Parent=MS.gene042416.t1
chr6.2 exon 81358740 81359370 81358740 ID=MS.gene042416.t1.exon2;Parent=MS.gene042416.t1
chr6.2 CDS 81358740 81359370 81358740 ID=cds.MS.gene042416.t1;Parent=MS.gene042416.t1
chr6.2 exon 81359904 81360133 81359904 ID=MS.gene042416.t1.exon3;Parent=MS.gene042416.t1
chr6.2 CDS 81359904 81360133 81359904 ID=cds.MS.gene042416.t1;Parent=MS.gene042416.t1
chr6.2 exon 81360249 81360320 81360249 ID=MS.gene042416.t1.exon4;Parent=MS.gene042416.t1
chr6.2 CDS 81360249 81360320 81360249 ID=cds.MS.gene042416.t1;Parent=MS.gene042416.t1
chr6.2 exon 81360404 81360424 81360404 ID=MS.gene042416.t1.exon5;Parent=MS.gene042416.t1
chr6.2 CDS 81360404 81360424 81360404 ID=cds.MS.gene042416.t1;Parent=MS.gene042416.t1
Gene Sequence

>MS.gene042416

ATGACAACCACTATACCTAGTGTTGGCATGGAGTTTGATAGTGTAGATGAAGCTTGGCAGTTTTGGGTTGAGTATGGTAAAGAAACTGGCTTTGGTGTTAGAAAGGATTATTTCAACAAAAAAAAGATAGACAGCTCCATTAGATCATGCAGGTTTGTTTGTTGCAAGGAGGGTCATCGGATACCGGACAAGAGATCTTATTTAGTTAAGAATCCTAGAGCTGAAACAAGGACTGATTGCAAAGCAAAAATTGCACTTAGTTCAAAGAATGGAAAGTTTGTAATCCATGAGTTTGTGAAGGACCATAACCACGATTTACAGCTTCCACAGACAACACACATGCTAGCATCACACCGTAAAATATCTGAAGTTCAAGCATATGAGATTGAAATGGCAGAGGACTCTGGTTTGAGGCAAAAGGCTTCATTTCAACTTATGAGTGCACAAGCAGGTGGTAGAACTAATCTTGGATATACACGGTTGGATGCTAAGAACTATCTTAAAGCAAGAAGACAAGTAACATGGTTTATGGGGAAGTTGGTAGTCTTATGAAGTATTTTCAACATCAGTTATTGAAGAATCCTTCATTTTTTTATGCACATCAGATGGGTTGCGATGAACAAATCACAAATGTATTTTGGGCTGATGCAAAAATGATCTTAGATTATGGATATTTTGGTGAGGTGGTTTCTTTAGATACTACGTATTGCACCAATCATGCTAATAGACCGTTGGCATTGTTTTCAGGCTTTAACCATTATAGAGGCATTGTCATTTTTGGGGCTGCACTCTTATATGATGAAACTGCTGAATCTTTCAAATGGTTATTTGAGGTATTTCTTGAAGCACATGGAAATAAAAAACCCCAAACTTTGTTCACAGATCAAGACCAGGCAATGTCTAAAGCACTAGCTGAAGTGATGCCAGAGACTCGACATGGGTTATGTACATGGCACTTGATGCAGAATGGTATAAAACACCTTGGTAACTTGATGAAAAAAGGAAGTCATTTCTTGGCTGATTTTAAGAAGTGCATGTATGATTATGATCAAGAGGAGAAGTTTGAGACAGCTTGGAGCAAGTTGGTTTCAGAGTTTAATGTCAATGAAAACAATTGGGTGAAGTCGATGTATGCTATTAAGGAAAAATGGGCAGCATGTCACATGAAGGAAGCATTCACTCTAGGAATGCGAAGCACTCAACTTAGTGAGAGTTTGAATTCTGATTTTAAGGCATGTATGACACCAAACATCAATGTTATTGATTTTTTCAAACACATTGAGAGAGTTGTGGAGGAAAAGCGAAACAACGAGTTAGTTTGTGAATATGAGGCTCGACATAAACTGCCAAATTGGATATACAGCCATTCACCAATTTTAGTGCAACTCGCGCAAATATACACACCTGCCATTTTTGACAAATTCCATAATGAATTTTCCTTATTTTTAGCTGCTCGCATAGAGAAAAAAATCTGTCCCAACTTCCATTTGAATATGTCATCACCTTGGTCAATAAGGAAGGAGAGTTTAAGGAAGGAGAGTGGAGAGTTCTATTTGATCCTGCACAATCATCTATATCATGTAGTTGTAGAAAGTTTGAGACTTTTGGAATACTTTGTTGCCATGCACTCAAGATCTTTGAGGCAAAGGAAAATGAGGTTGTGGAAGATCCCAAATTATACATGATGCAACGATATAGGCGACTTTGATCAACAATGATTAGATTGGCAGCCGATGTATCCACATCTCCTACATTATATGAACTTGCAGACAATGTAATACATGACTTGTGTAAAAAAGTGATGGAGGTTAGACTAGAACAAAATGCAACGGTCAATGATGAAGGTGGTGTTTCGACATTGGCTGAACCGATTGAGACAACAGCAAAGGGGTATAAGAATCGGCGTGGGTCAAAGAGACAAAAGCGATTCAAAAGTTGGATTGAACGTCAGCCTAAAAGAGCTCCGATTGTACAACCTTCGTCTTGTCATCAATCAAGGGTATATAAGTTTATTGTTGTAACAATTTTTCTTTCAAAATAATTATTTTTCAATTATTGTGTATTAATTTTTAAAATCATTGAAGGTTGGATAATCAAGTCAGATCGTCTTTCAGACATCAGGTGTGGCACATCCAACACATGAGTTATCTCAAACAAATCAATTTAGTTTCACTAGATTATTAATGGTAAGATTCCTTGGTCTATAAAAAGTAATGGTTATGTTTTATTTCCAACTTTTTATGATATTGTGTATGTGTTTTTAATGTAGGAGTCAATTGAACATGAATGA
Protein sequence

>MS.gene042416.t1

MTTTIPSVGMEFDSVDEAWQFWVEYGKETGFGVRKDYFNKKKIDSSIRSCRFVCCKEGHRIPDKRSYLVKNPRAETRTDCKAKIALSSKNGKFVIHEFVKDHNHDLQLPQTTHMLASHRKISEVQAYEIEMAEDSGLRQKASFQLMSAQAGGRTNLGYTRLDAKNYLKARRQMGCDEQITNVFWADAKMILDYGYFGEVVSLDTTYCTNHANRPLALFSGFNHYRGIVIFGAALLYDETAESFKWLFEVFLEAHGNKKPQTLFTDQDQAMSKALAEVMPETRHGLCTWHLMQNGIKHLGNLMKKGSHFLADFKKCMYDYDQEEKFETAWSKLVSEFNVNENNWVKSMYAIKEKWAACHMKEAFTLGMRSTQLSESLNSDFKAYNVIHDLCKKVMEVRLEQNATVNDEGGVSTLAEPIETTAKGYKNRRGSKRQKRFKSWIERQPKRAPIVQPSSCHQSRTSGVAHPTHELSQTNQFSFTRLLMESIEHE