AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04250.t1 MTR_3g464290 78.761 452 62 4 583 1034 54 471 0.0 677
MS.gene04250.t1 MTR_3g464290 59.722 216 66 2 213 407 228 443 5.96e-65 227
MS.gene04250.t1 MTR_3g464270 66.832 404 74 7 1 400 1 348 2.16e-167 495
MS.gene04250.t1 MTR_3g464270 60.053 378 121 5 628 1004 8 356 1.85e-137 417
MS.gene04250.t1 MTR_1g075890 55.579 484 144 10 587 1016 187 653 2.02e-140 436
MS.gene04250.t1 MTR_1g075890 39.879 494 179 15 8 474 228 630 1.15e-78 271
MS.gene04250.t1 MTR_3g464260 48.889 450 134 9 585 1034 162 515 3.27e-110 352
MS.gene04250.t1 MTR_3g464260 37.155 471 135 12 39 502 213 529 1.70e-65 231
MS.gene04250.t1 MTR_3g464280 52.010 398 135 9 592 988 151 493 7.06e-102 329
MS.gene04250.t1 MTR_3g464280 39.951 408 126 11 8 397 187 493 1.23e-56 204
MS.gene04250.t1 MTR_5g071070 43.307 508 206 17 595 1034 115 608 1.95e-98 324
MS.gene04250.t1 MTR_5g071070 36.451 417 184 14 8 380 147 526 3.09e-55 203
MS.gene04250.t1 MTR_5g071070 43.307 508 206 17 595 1034 197 690 6.66e-97 322
MS.gene04250.t1 MTR_5g071070 36.451 417 184 14 8 380 229 608 5.56e-55 204
MS.gene04250.t1 MTR_5g082830 39.917 481 208 14 608 1025 196 658 2.66e-84 287
MS.gene04250.t1 MTR_5g082830 33.732 418 197 13 8 382 216 596 7.51e-46 176
MS.gene04250.t1 MTR_3g070880 39.220 436 171 16 593 1006 122 485 8.82e-71 245
MS.gene04250.t1 MTR_3g070880 61.017 118 43 2 8 123 162 278 4.77e-42 162
MS.gene04250.t1 MTR_7g092070 35.345 464 210 12 606 1006 241 677 1.46e-64 233
MS.gene04250.t1 MTR_7g092070 46.316 190 87 3 8 183 263 451 4.11e-44 172
MS.gene04250.t1 MTR_3g029590 43.110 283 130 7 606 863 241 517 2.70e-60 220
MS.gene04250.t1 MTR_3g029590 43.781 201 89 5 8 190 263 457 3.16e-43 169
MS.gene04250.t1 MTR_1g050438 38.989 277 141 6 606 863 205 472 1.16e-50 189
MS.gene04250.t1 MTR_1g050438 46.043 139 67 3 8 144 227 359 1.20e-32 135
MS.gene04250.t1 MTR_0728s0020 38.989 277 141 6 606 863 221 488 2.43e-50 189
MS.gene04250.t1 MTR_0728s0020 46.043 139 67 3 8 144 243 375 1.63e-32 135
MS.gene04250.t1 MTR_3g435680 49.718 177 83 4 606 781 130 301 7.12e-48 176
MS.gene04250.t1 MTR_3g435680 46.853 143 67 3 8 142 150 291 3.37e-37 145
MS.gene04250.t1 MTR_3g435590 52.353 170 73 2 607 776 216 377 2.33e-47 177
MS.gene04250.t1 MTR_3g435590 51.064 141 56 4 8 136 237 376 5.48e-36 143
MS.gene04250.t1 MTR_6g090380 56.881 109 46 1 625 733 99 206 5.19e-40 152
MS.gene04250.t1 MTR_6g090380 55.238 105 44 2 8 110 102 205 7.83e-34 134
MS.gene04250.t1 MTR_8g102100 52.344 128 60 1 625 752 77 203 5.40e-40 153
MS.gene04250.t1 MTR_8g102100 51.587 126 58 2 7 130 79 203 9.39e-37 143
MS.gene04250.t1 MTR_3g103960 51.908 131 60 3 619 747 43 172 3.86e-39 147
MS.gene04250.t1 MTR_3g103960 53.846 117 52 1 8 122 52 168 3.59e-37 141
MS.gene04250.t1 MTR_2g090605 55.357 112 49 1 629 740 77 187 5.21e-39 150
MS.gene04250.t1 MTR_2g090605 50.893 112 52 2 9 118 77 187 6.84e-31 127
MS.gene04250.t1 MTR_4g078660 55.046 109 48 1 625 733 56 163 4.83e-37 142
MS.gene04250.t1 MTR_4g078660 49.524 105 50 2 8 110 59 162 5.83e-29 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04250.t1 AT5G58620 41.346 416 212 13 606 1006 186 584 2.22e-80 275
MS.gene04250.t1 AT5G58620 45.918 196 87 6 8 190 208 397 2.39e-44 171
MS.gene04250.t1 AT2G40140 39.702 403 199 11 611 1009 196 558 4.15e-73 254
MS.gene04250.t1 AT2G40140 48.691 191 84 6 8 190 213 397 9.91e-44 169
MS.gene04250.t1 AT2G40140 39.702 403 199 11 611 1009 196 558 4.15e-73 254
MS.gene04250.t1 AT2G40140 48.691 191 84 6 8 190 213 397 9.91e-44 169
MS.gene04250.t1 AT3G55980 41.439 403 181 17 611 1006 193 547 1.44e-70 246
MS.gene04250.t1 AT3G55980 37.987 308 111 14 8 311 210 441 1.83e-41 162
MS.gene04250.t1 AT3G55980 41.439 403 181 17 611 1006 260 614 4.23e-70 247
MS.gene04250.t1 AT3G55980 37.987 308 111 14 8 311 277 508 4.22e-41 162
MS.gene04250.t1 AT2G41900 46.642 268 120 5 608 863 240 496 1.08e-64 233
MS.gene04250.t1 AT2G41900 37.979 287 113 8 8 286 260 489 6.95e-45 174
MS.gene04250.t1 AT5G12850 37.349 415 200 16 606 970 234 638 1.32e-63 229
MS.gene04250.t1 AT5G12850 37.374 297 126 11 8 293 256 503 6.33e-42 165
MS.gene04250.t1 AT5G07500 58.197 122 50 1 611 732 34 154 1.81e-45 164
MS.gene04250.t1 AT5G07500 52.381 105 47 2 8 110 51 154 4.81e-32 125
MS.gene04250.t1 AT2G25900 60.909 110 42 1 625 734 86 194 1.73e-42 158
MS.gene04250.t1 AT2G25900 49.606 127 61 2 8 132 89 214 2.33e-35 137
MS.gene04250.t1 AT2G25900 60.909 110 42 1 625 734 86 194 1.73e-42 158
MS.gene04250.t1 AT2G25900 49.606 127 61 2 8 132 89 214 2.33e-35 137
MS.gene04250.t1 AT2G19810 47.712 153 64 2 614 766 63 199 7.50e-42 157
MS.gene04250.t1 AT2G19810 52.336 107 48 2 8 112 77 182 1.29e-32 130
MS.gene04250.t1 AT4G29190 55.556 117 51 1 618 734 68 183 7.06e-41 155
MS.gene04250.t1 AT4G29190 44.595 148 79 2 8 153 78 224 2.41e-35 139
MS.gene04250.t1 AT1G03790 51.538 130 58 3 622 747 75 203 3.52e-38 148
MS.gene04250.t1 AT1G03790 51.667 120 53 2 8 122 81 200 6.24e-34 135
MS.gene04250.t1 AT5G44260 51.163 129 57 3 607 734 42 165 5.78e-38 147
MS.gene04250.t1 AT5G44260 54.206 107 47 1 8 112 59 165 1.01e-33 134

Find 243 sgRNAs with CRISPR-Local

Find 366 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAACCTTGAAGGCTATTT+TGG 0.068267 3.4:+59398891 MS.gene04250:CDS
GAATGTCCCTTTGCTCATTC+TGG 0.150818 3.4:+59403055 MS.gene04250:CDS
TATATATTACAGATGAATTT+AGG 0.242480 3.4:+59402975 MS.gene04250:CDS
ACGGAACATCGTCCGGATTA+TGG 0.252268 3.4:-59404182 None:intergenic
AAATAGCCTTCAAGGTTAAT+AGG 0.274059 3.4:-59398888 None:intergenic
CTTCTCCCTTGAGGCAATCT+TGG 0.284448 3.4:-59402853 None:intergenic
AGAACAAAGCTTTGCAATAA+TGG 0.287623 3.4:+59403223 MS.gene04250:CDS
AGAACAACGCTTTGCAATAA+TGG 0.289322 3.4:+59398306 MS.gene04250:CDS
TCATAGCTTGAATTCATAAA+TGG 0.296275 3.4:-59403796 None:intergenic
GAAGAGTCAAATAGGCTTGC+AGG 0.316240 3.4:+59403619 MS.gene04250:CDS
CTTCTACTGCCTTCTCTGAT+TGG 0.322323 3.4:+59403914 MS.gene04250:CDS
CATTATTAAGCCAGGAATCT+TGG 0.322794 3.4:+59404079 MS.gene04250:CDS
CTATTTGACTCTTCTATCAT+TGG 0.335001 3.4:-59403610 None:intergenic
CTATCATTGGCTTCGGCATA+TGG 0.338840 3.4:-59403597 None:intergenic
AAGGGTAGTCACACAGTTCT+TGG 0.351571 3.4:-59403723 None:intergenic
TAATCTTTCTTCTTAGTTTG+AGG 0.353033 3.4:-59402914 None:intergenic
TGGTTAGATCACAACAGTTC+AGG 0.360050 3.4:+59399006 MS.gene04250:CDS
CAATGATAGAAGAGTCAAAT+AGG 0.360308 3.4:+59403611 MS.gene04250:CDS
TTCAGCCAAAGGTTATACTA+TGG 0.366113 3.4:+59400792 MS.gene04250:CDS
GACTCTTCTATCATTGGCTT+CGG 0.369106 3.4:-59403604 None:intergenic
CTACTTGACCCTTCTATCAT+TGG 0.369851 3.4:-59398849 None:intergenic
ATGCCGAATCCAATGATAGA+AGG 0.372921 3.4:+59398840 MS.gene04250:CDS
TTCACCTAGTGTTTCTTCAA+TGG 0.380977 3.4:+59403354 MS.gene04250:CDS
TGATCCAATCACATTTGAAT+TGG 0.381273 3.4:-59403755 None:intergenic
GAAGGGTCAAGTAGGCTTGC+TGG 0.383335 3.4:+59398858 MS.gene04250:CDS
GTAAGGAATCATACAGTAAA+GGG 0.386561 3.4:-59400950 None:intergenic
AATATAGATCATGCAGTAAA+GGG 0.387652 3.4:+59398226 MS.gene04250:CDS
GTGAGCTAACCCAAGATTCC+TGG 0.390717 3.4:-59404089 None:intergenic
TGATCTAACCACATTAGAAT+TGG 0.391379 3.4:-59398994 None:intergenic
TTGGTGAAGGTTTAGAAACT+TGG 0.392413 3.4:-59402091 None:intergenic
GACCCTTCTATCATTGGATT+CGG 0.397498 3.4:-59398843 None:intergenic
GGCAAAGACGAAACCGCGTC+TGG 0.398787 3.4:-59401267 None:intergenic
TGCTGCTCTCGATAAAGTTC+TGG 0.399385 3.4:-59399076 None:intergenic
TACTACAAGGATATAGTATA+TGG 0.402818 3.4:+59401533 MS.gene04250:CDS
AAACTAAGAAGAAAGATTAT+CGG 0.403018 3.4:+59402918 MS.gene04250:CDS
CCGTGATAGAAAATTAGAAT+TGG 0.404340 3.4:+59399175 MS.gene04250:CDS
TCGATCCCTACCTGACATAA+AGG 0.406902 3.4:+59402946 MS.gene04250:CDS
TGACTAGTGCAGCAGCTTGG+TGG 0.409389 3.4:+59401989 MS.gene04250:CDS
CAATCACATTTGAATTGGTT+AGG 0.412888 3.4:-59403750 None:intergenic
ATGACCCGAAAATGTCTTCA+TGG 0.413038 3.4:-59403657 None:intergenic
TTCAACAGGCGAAGCTCCTC+GGG 0.413095 3.4:-59403286 None:intergenic
CGAGGAGCTTCGCCTGTTGA+AGG 0.414602 3.4:+59403288 MS.gene04250:CDS
AAATGTCCCTTTGCTCATCA+TGG 0.414986 3.4:+59398135 MS.gene04250:CDS
AATGTCCCTTTGCTCATTCT+GGG 0.417384 3.4:+59403056 MS.gene04250:CDS
AAAATGTCTTCATGGTTAGT+AGG 0.419191 3.4:-59403649 None:intergenic
GCACATGGTGTTCTTGAGTC+CGG 0.419484 3.4:+59398264 MS.gene04250:CDS
ATTGCGGTTATGTTCATGAT+TGG 0.421921 3.4:+59398109 MS.gene04250:CDS
TCTAAACCTTCACCAAGTTT+CGG 0.422466 3.4:+59402098 MS.gene04250:CDS
ATGACCCAAAATAGCCTTCA+AGG 0.426670 3.4:-59398896 None:intergenic
ATTATTAAGCCAGGAATCTT+GGG 0.427376 3.4:+59404080 MS.gene04250:CDS
GGCCACATGGTTCTTTCTGC+AGG 0.427795 3.4:-59398696 None:intergenic
TGCTCCATGTACAATTTCTC+TGG 0.430560 3.4:-59401288 None:intergenic
TGGCGTGGATCTTCAACATC+TGG 0.431502 3.4:-59401234 None:intergenic
CTCATTCTGGGGAAATTGCA+AGG 0.434253 3.4:+59403068 MS.gene04250:CDS
TGCCACATGGTTCCTCCTGC+AGG 0.436089 3.4:-59403457 None:intergenic
CTAACCAATTCAAATGTGAT+TGG 0.436311 3.4:+59403751 MS.gene04250:CDS
AATTATATTGCTTCGGATCA+CGG 0.437370 3.4:-59398173 None:intergenic
GGCTAGGACGGAACATCGTC+CGG 0.437872 3.4:-59404189 None:intergenic
GAGCCTTCAACTGCCTTCTC+CGG 0.438785 3.4:+59399143 MS.gene04250:CDS
AACTGGTATAAGCCTTCAAC+AGG 0.439127 3.4:-59403300 None:intergenic
TTCGACCATAGTATAACCTT+TGG 0.440917 3.4:-59400797 None:intergenic
TACATCAAATGTTGATGATC+AGG 0.442359 3.4:+59399229 MS.gene04250:CDS
TGCTAGAGATAATACTCATC+TGG 0.443782 3.4:+59398791 MS.gene04250:CDS
GTGTCGATAATACAAAAGTT+AGG 0.444425 3.4:+59401471 MS.gene04250:CDS
ATGACGACGTCTTATTCATT+CGG 0.444646 3.4:+59403991 MS.gene04250:CDS
GACTAGTGCAGCAGCTTGGT+GGG 0.446802 3.4:+59401990 MS.gene04250:CDS
CAATCAGAGAAGGCAGTAGA+AGG 0.448046 3.4:-59403913 None:intergenic
CAGCTGTAATAATATTTCTT+CGG 0.448097 3.4:-59403100 None:intergenic
TTTCTGAAATTCATTACAAT+GGG 0.449878 3.4:-59398205 None:intergenic
ACAATCTCCGACAGCAATGC+AGG 0.452955 3.4:+59398923 MS.gene04250:CDS
TTCAACGGGCGAAGTTCCTC+AGG 0.454486 3.4:-59398369 None:intergenic
TCTATCACGGGAGCGCCAAC+CGG 0.454780 3.4:-59399162 None:intergenic
CATCTGTAATTATATTGCTT+CGG 0.456547 3.4:-59398180 None:intergenic
TTGCTCGGCATCTGAGGTGT+AGG 0.459489 3.4:-59398567 None:intergenic
TTGCTCGGCATCTGAGGTGT+AGG 0.459489 3.4:-59398657 None:intergenic
TTGCTCGGCATCTGAGGTGT+AGG 0.459489 3.4:-59398747 None:intergenic
CGTGATAGAAAATTAGAATT+GGG 0.460547 3.4:+59399176 MS.gene04250:CDS
AGATTCTACCTCAGTTGCAT+TGG 0.462466 3.4:+59399121 MS.gene04250:CDS
TTGCTTGGCATCTGAAGTGT+GGG 0.465030 3.4:-59403508 None:intergenic
AACTGGTACTAGCATTCAAC+GGG 0.465209 3.4:-59398383 None:intergenic
ATGTGAAGGCAAAGCAGAAC+TGG 0.466124 3.4:-59398400 None:intergenic
CTTCAACAGGCGAAGCTCCT+CGG 0.466146 3.4:-59403287 None:intergenic
CTTCAACTGCCTTCTCCGGT+TGG 0.466199 3.4:+59399147 MS.gene04250:CDS
TGCTGTGCTCGATAAAGCTC+TGG 0.466382 3.4:-59403837 None:intergenic
AATGCAACTGAGGTAGAATC+TGG 0.470321 3.4:-59399119 None:intergenic
CAACCGGAGAAGGCAGTTGA+AGG 0.471582 3.4:-59399146 None:intergenic
TGTTGATCAGATTCCAATGT+TGG 0.474488 3.4:-59404129 None:intergenic
CAGAATGGATCATGCAGTAG+AGG 0.475417 3.4:+59403142 MS.gene04250:CDS
AGGTGAAGGAAAAGCAGAAC+TGG 0.477528 3.4:-59403317 None:intergenic
CGTAAGGAATCATACAGTAA+AGG 0.478110 3.4:-59400951 None:intergenic
AAATATAGATCATGCAGTAA+AGG 0.479750 3.4:+59398225 MS.gene04250:CDS
CATTTCATTGAGTTTATCAC+CGG 0.482092 3.4:-59403971 None:intergenic
CAAGATATGATGCCCTTTCG+AGG 0.483484 3.4:+59399048 MS.gene04250:CDS
GGCCACATGGTTCTTCCTGC+AGG 0.483695 3.4:-59398516 None:intergenic
GGCCACATGGTTCTTCCTGC+AGG 0.483695 3.4:-59398606 None:intergenic
TACTATGGTCGAAGCTCCTC+GGG 0.485890 3.4:+59400807 MS.gene04250:CDS
GTGAATCCTATTAACCTTGA+AGG 0.487609 3.4:+59398882 MS.gene04250:CDS
GAATTATTCATTATTAAGCC+AGG 0.489175 3.4:+59404071 MS.gene04250:CDS
TTTGAATTGGTTAGGTCAGA+AGG 0.489977 3.4:-59403742 None:intergenic
ATTTCTGAAATTCATTACAA+TGG 0.490595 3.4:-59398206 None:intergenic
CAAAGTGAAGCTTTGCTCAT+GGG 0.492005 3.4:+59403009 MS.gene04250:CDS
CCACTGATAGAAAGGTAGAT+TGG 0.492569 3.4:+59403941 MS.gene04250:CDS
GGTTAGATCACAACAGTTCA+GGG 0.493239 3.4:+59399007 MS.gene04250:CDS
AAGGAAACTGCACCGAAACT+TGG 0.493835 3.4:-59402110 None:intergenic
ACGGGAGCGCCAACCGGAGA+AGG 0.494199 3.4:-59399156 None:intergenic
ATACTATGGTCGAAGCTCCT+CGG 0.494840 3.4:+59400806 MS.gene04250:CDS
AATCTTCGTCGACACTGCCT+TGG 0.499906 3.4:+59403413 MS.gene04250:CDS
GCTCTGGCTCCGATTCGAAA+TGG 0.500616 3.4:-59403821 None:intergenic
GTCCAAGGCGGCATCGATGA+AGG 0.500707 3.4:-59398474 None:intergenic
GGTGAATTTGAATTTGATGT+AGG 0.501254 3.4:-59403337 None:intergenic
TCAAAGTGAAGCTTTGCTCA+TGG 0.502823 3.4:+59403008 MS.gene04250:CDS
AATCTGTTGCTCGGCATCTG+AGG 0.505565 3.4:-59398753 None:intergenic
AATAATATTTCTTCGGATCA+CGG 0.506985 3.4:-59403093 None:intergenic
TCAACTACAATGCCATAATC+CGG 0.507140 3.4:+59404170 MS.gene04250:CDS
AATGTCCCTTTGCTCATCAT+GGG 0.509346 3.4:+59398136 MS.gene04250:CDS
TGAGGTAGAATCTGGAAGCT+TGG 0.511466 3.4:-59399111 None:intergenic
TGCCTTCATCGATGCCGCCT+TGG 0.512659 3.4:+59398472 MS.gene04250:CDS
CCGCGTCTGGATTATAGCAT+TGG 0.513347 3.4:-59401254 None:intergenic
TATATCCTATCCTTTATGTC+AGG 0.513817 3.4:-59402956 None:intergenic
CAAGGGAGAAGGTTTCGAAA+TGG 0.520068 3.4:+59402865 MS.gene04250:CDS
TATTGTCATGAATTTCAGAA+TGG 0.523011 3.4:+59403127 MS.gene04250:CDS
CAAGATTGCCTCAAGGGAGA+AGG 0.523740 3.4:+59402854 MS.gene04250:CDS
GCTTTATTGAGAGCAATAGC+GGG 0.524886 3.4:+59401092 MS.gene04250:CDS
TATTGCTCTCAATAAAGCTC+TGG 0.525656 3.4:-59401087 None:intergenic
CAGAACCAAGATTGCCTCAA+GGG 0.525928 3.4:+59402848 MS.gene04250:intron
CACAATTGCTCTGGCTAGGA+CGG 0.526993 3.4:-59404201 None:intergenic
AATCTGTTGCTCGGCATCTG+AGG 0.528491 3.4:-59398573 None:intergenic
AATCTGTTGCTCGGCATCTG+AGG 0.528491 3.4:-59398663 None:intergenic
GTTGCTTGGCATCTGAAGTG+TGG 0.528652 3.4:-59403509 None:intergenic
CACATGGTGTTCTTGAGTCC+GGG 0.529152 3.4:+59398265 MS.gene04250:CDS
GATGGATTATTAAGATATGA+AGG 0.531625 3.4:+59399251 MS.gene04250:CDS
AAGGATATATCTGAAGTTGT+TGG 0.532786 3.4:-59398962 None:intergenic
CTTTCCCCATGATGAGCAAA+GGG 0.533381 3.4:-59398141 None:intergenic
TTGGTACTCCACTGATAGAA+AGG 0.535177 3.4:+59403933 MS.gene04250:CDS
TTGGGTTAGCTCACTTGTGA+AGG 0.535289 3.4:+59404098 MS.gene04250:CDS
TATGATATCAATCTGTTGCT+CGG 0.538210 3.4:-59398582 None:intergenic
TATGATATCAATCTGTTGCT+CGG 0.538210 3.4:-59398672 None:intergenic
AGTTCTGGCTCTGCCTCGAA+AGG 0.544056 3.4:-59399061 None:intergenic
GCTCTGGCTCCGTTTCGAAA+AGG 0.548920 3.4:-59401071 None:intergenic
TAACCACATTAGAATTGGTA+AGG 0.551193 3.4:-59398989 None:intergenic
TGCTCGGCATCTGAGGTGTA+GGG 0.552789 3.4:-59398566 None:intergenic
TGCTCGGCATCTGAGGTGTA+GGG 0.552789 3.4:-59398656 None:intergenic
TGCTCGGCATCTGAGGTGTA+GGG 0.552789 3.4:-59398746 None:intergenic
ACAATCTTCGAAAGACATGC+TGG 0.555471 3.4:+59403684 MS.gene04250:CDS
CCTATCCTTTATGTCAGGTA+GGG 0.557883 3.4:-59402951 None:intergenic
ATTTCCCCAGAATGAGCAAA+GGG 0.558937 3.4:-59403061 None:intergenic
TCTGCAACATCATCTCCTGC+AGG 0.561276 3.4:+59403442 MS.gene04250:CDS
AGCTTTATTGAGAGCAATAG+CGG 0.562255 3.4:+59401091 MS.gene04250:CDS
TTTGCCAGAGAAATTGTACA+TGG 0.563978 3.4:+59401284 MS.gene04250:CDS
GCTATGATGCCATTTCGAAT+CGG 0.565131 3.4:+59403812 MS.gene04250:CDS
AGAATGGATCATGCAGTAGA+GGG 0.565871 3.4:+59403143 MS.gene04250:CDS
GCAGTATTCAATCTGTTGCT+CGG 0.567984 3.4:-59398762 None:intergenic
TCACCTTACCAATTCTAATG+TGG 0.568314 3.4:+59398986 MS.gene04250:CDS
CACTAGCAACACTTCAACAA+TGG 0.568642 3.4:+59399202 MS.gene04250:CDS
GATGTTGCAGACGGAGTCCA+AGG 0.569189 3.4:-59403430 None:intergenic
AAACATTGCTAGTATCGTCT+AGG 0.570465 3.4:-59404017 None:intergenic
TTAGAATTGGTAAGGTGAGA+AGG 0.571244 3.4:-59398981 None:intergenic
GATATTGCAGACGGTGTCCA+AGG 0.573927 3.4:-59398489 None:intergenic
CCAATCTACCTTTCTATCAG+TGG 0.576349 3.4:-59403941 None:intergenic
TTTGAATTTGATGTAGGTGA+AGG 0.577407 3.4:-59403331 None:intergenic
AAACAATGCAAAGGAAGAAG+TGG 0.578510 3.4:-59402067 None:intergenic
GCTTTCCCCATGATGAGCAA+AGG 0.578685 3.4:-59398142 None:intergenic
CAATGATAGAAGGGTCAAGT+AGG 0.578738 3.4:+59398850 MS.gene04250:CDS
GTATACATTGCAGAAGCACT+TGG 0.582995 3.4:-59398441 None:intergenic
CTTCTTTGCTCACAAACCTG+AGG 0.585534 3.4:+59398353 MS.gene04250:CDS
TGCCGAATCCAATGATAGAA+GGG 0.587494 3.4:+59398841 MS.gene04250:CDS
TCCTATCCTTTATGTCAGGT+AGG 0.588316 3.4:-59402952 None:intergenic
GTACTAATCTGAGTCGGAGT+CGG 0.588992 3.4:-59398717 None:intergenic
TTTCGAAACCTTCTCCCTTG+AGG 0.589169 3.4:-59402862 None:intergenic
TTGAATTGGTTAGGTCAGAA+GGG 0.590246 3.4:-59403741 None:intergenic
GTTCTGGCTCTGCCTCGAAA+GGG 0.592388 3.4:-59399060 None:intergenic
CTGAGTCGGAGTCGGCCACA+TGG 0.594746 3.4:-59398529 None:intergenic
CTGAGTCGGAGTCGGCCACA+TGG 0.594746 3.4:-59398619 None:intergenic
CAATCTCCGACAGCAATGCA+GGG 0.598628 3.4:+59398924 MS.gene04250:CDS
GTACGATTCTGAGTCGGAGT+CGG 0.598804 3.4:-59398537 None:intergenic
GTACGATTCTGAGTCGGAGT+CGG 0.598804 3.4:-59398627 None:intergenic
GAACTGGTACTAGCATTCAA+CGG 0.603434 3.4:-59398384 None:intergenic
TCAAATGTTGATGATCAGGA+TGG 0.604566 3.4:+59399233 MS.gene04250:CDS
CTGAGTCGGAGTCGGCCACA+TGG 0.607724 3.4:-59398709 None:intergenic
AGATTGATATCATATCCTGC+AGG 0.608072 3.4:+59398591 MS.gene04250:CDS
AAACTTGGAAAACAATGCAA+AGG 0.609298 3.4:-59402076 None:intergenic
AAGGTAGATTGGTCTATCCC+CGG 0.610542 3.4:+59403952 MS.gene04250:CDS
TGATACCCCTGCATTGCTGT+CGG 0.611093 3.4:-59398930 None:intergenic
GGACATTCTGTCCACTCATG+AGG 0.613065 3.4:-59403040 None:intergenic
ATGTCCCTTTGCTCATTCTG+GGG 0.613840 3.4:+59403057 MS.gene04250:CDS
TCTGCAATATCATATCCTGC+AGG 0.614316 3.4:+59398501 MS.gene04250:CDS
CAGTGACTAGTGCAGCAGCT+TGG 0.614391 3.4:+59401986 MS.gene04250:intron
CTTCTTTGCTCACAAACCCG+AGG 0.614714 3.4:+59403270 MS.gene04250:CDS
AACAACTTGTTACTACTACA+AGG 0.615693 3.4:+59401520 MS.gene04250:CDS
TACTAGCAATGTTTCAACAA+TGG 0.618393 3.4:+59404026 MS.gene04250:CDS
ACTGTAGTACTAATCTGAGT+CGG 0.618849 3.4:-59398723 None:intergenic
AATGCAACTGAGGTAGCAGA+AGG 0.619250 3.4:-59403886 None:intergenic
ACTGTAGTATGAATCTGAGT+CGG 0.620110 3.4:-59403484 None:intergenic
GAAGATTGTATCAAAGAAGA+CGG 0.623170 3.4:-59403397 None:intergenic
CCCTACCTGACATAAAGGAT+AGG 0.627726 3.4:+59402951 MS.gene04250:CDS
GGGATAATACTCCTCATGAG+TGG 0.631269 3.4:+59403029 MS.gene04250:CDS
ACATTCAAAGTGCGGCATTG+CGG 0.631811 3.4:+59398093 MS.gene04250:CDS
ACAGAACCAAGATTGCCTCA+AGG 0.634336 3.4:+59402847 MS.gene04250:intron
TGATGTATACATTCAAAGTG+CGG 0.634386 3.4:+59398085 MS.gene04250:CDS
AATTTCCCCAGAATGAGCAA+AGG 0.634761 3.4:-59403062 None:intergenic
GCAGGAGATGATGTTGCAGA+CGG 0.635347 3.4:-59403439 None:intergenic
AGCTTTGTTCTGTATCGACT+AGG 0.635835 3.4:-59403211 None:intergenic
CTTTATCGAGAGCAGCAGCA+TGG 0.636540 3.4:+59399082 MS.gene04250:CDS
AGTGGAGTACCAATCAGAGA+AGG 0.636735 3.4:-59403923 None:intergenic
TTTGAATATAATGTATGTGA+AGG 0.637611 3.4:-59398414 None:intergenic
GAGAAGCAGAAACAACATGT+AGG 0.638851 3.4:+59402887 MS.gene04250:CDS
GTACATGGAGCATAAGCAGA+AGG 0.641793 3.4:+59401299 MS.gene04250:CDS
GCAGGATATGATATTGCAGA+CGG 0.643899 3.4:-59398498 None:intergenic
ATATAGATCATGCAGTAAAG+GGG 0.646866 3.4:+59398227 MS.gene04250:CDS
GAAGGATGATGATCCAACAT+TGG 0.647091 3.4:+59404116 MS.gene04250:CDS
CTGAGTCGGAGTCTGCCACA+TGG 0.648294 3.4:-59403470 None:intergenic
GAATCCATTGAAGAAACACT+AGG 0.648660 3.4:-59403358 None:intergenic
TGAAGGCTCCAATGCAACTG+AGG 0.649434 3.4:-59399129 None:intergenic
TAAGGAATCATACAGTAAAG+GGG 0.650413 3.4:-59400949 None:intergenic
ATGTCCCTTTGCTCATCATG+GGG 0.650802 3.4:+59398137 MS.gene04250:CDS
AAGCAGAAACAACATGTAGG+TGG 0.652560 3.4:+59402890 MS.gene04250:CDS
ACTGTAGTACGATTCTGAGT+CGG 0.653294 3.4:-59398543 None:intergenic
ACTGTAGTACGATTCTGAGT+CGG 0.653294 3.4:-59398633 None:intergenic
AGAAGGCTCTAATGCAACTG+AGG 0.653652 3.4:-59403896 None:intergenic
ACTGCACCGAAACTTGGTGA+AGG 0.655406 3.4:-59402104 None:intergenic
TCTGGATTATAGCATTGGCG+TGG 0.657723 3.4:-59401249 None:intergenic
GCAACATCATCTCCTGCAGG+AGG 0.663953 3.4:+59403445 MS.gene04250:CDS
GTATCGACTAGGATGCAGCC+CGG 0.668994 3.4:-59398283 None:intergenic
GTATCGACTAGGATGCAGCC+CGG 0.668994 3.4:-59403200 None:intergenic
GAATGGATCATGCAGTAGAG+GGG 0.673342 3.4:+59403144 MS.gene04250:CDS
ATTTCATTGAGTTTATCACC+GGG 0.674570 3.4:-59403970 None:intergenic
TGCTTGGCATCTGAAGTGTG+GGG 0.675981 3.4:-59403507 None:intergenic
AGCGTTGTTCTGTATCGACT+AGG 0.678911 3.4:-59398294 None:intergenic
CTTTATTGAGAGCAATAGCG+GGG 0.684685 3.4:+59401093 MS.gene04250:CDS
AATGGATCAACGCTGAGTTG+TGG 0.685904 3.4:-59403778 None:intergenic
AATCTCCGACAGCAATGCAG+GGG 0.691632 3.4:+59398925 MS.gene04250:CDS
CTTTATCGAGCACAGCAGCG+TGG 0.707372 3.4:+59403843 MS.gene04250:CDS
GTTAGATCACAACAGTTCAG+GGG 0.713618 3.4:+59399008 MS.gene04250:CDS
ATTGCAGACGGTGTCCAAGG+CGG 0.715505 3.4:-59398486 None:intergenic
GATACATGTCAATATTCACA+TGG 0.715825 3.4:+59403166 MS.gene04250:CDS
GATGCTTGTGAATATGCACA+CGG 0.727347 3.4:-59400927 None:intergenic
TTTCATTGAGTTTATCACCG+GGG 0.737072 3.4:-59403969 None:intergenic
CTCCTGCAGGAGGAACCATG+TGG 0.747414 3.4:+59403455 MS.gene04250:CDS
GATGAATGTCAATATGCACA+TGG 0.762142 3.4:+59398249 MS.gene04250:CDS
CCAATGCTATAATCCAGACG+CGG 0.765540 3.4:+59401254 MS.gene04250:CDS
ATCCTGCAGGAAGAACCATG+TGG 0.814597 3.4:+59398514 MS.gene04250:CDS
ATCCTGCAGGAAGAACCATG+TGG 0.814597 3.4:+59398604 MS.gene04250:CDS
ATCCTGCAGAAAGAACCATG+TGG 0.834161 3.4:+59398694 MS.gene04250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGATCTTAATTAAATAT+AGG + chr3.4:59401422-59401441 MS.gene04250:intron 10.0%
!! AATAACAAAACAAAGAATTA+AGG - chr3.4:59399496-59399515 None:intergenic 15.0%
!! ATAAAAATAATCACAAACAA+TGG - chr3.4:59402729-59402748 None:intergenic 15.0%
!! ATAACAAAACAAAGAATTAA+GGG - chr3.4:59399495-59399514 None:intergenic 15.0%
!! TAGAGATATTCATTATATTT+GGG + chr3.4:59399417-59399436 MS.gene04250:intron 15.0%
!! TATATATTACAGATGAATTT+AGG + chr3.4:59402975-59402994 MS.gene04250:CDS 15.0%
!! TCATATCTTGAATTAAAAAA+TGG - chr3.4:59399038-59399057 None:intergenic 15.0%
!! TTAGAGATATTCATTATATT+TGG + chr3.4:59399416-59399435 MS.gene04250:intron 15.0%
!! TTATATTGAAGAAAGAAATT+CGG - chr3.4:59401795-59401814 None:intergenic 15.0%
!! TTTAAACCTTATGAATAAAA+TGG - chr3.4:59400071-59400090 None:intergenic 15.0%
!! AAACTAAGAAGAAAGATTAT+CGG + chr3.4:59402918-59402937 MS.gene04250:CDS 20.0%
!! AAAGAAAAAAGAAAACAAAG+CGG - chr3.4:59400372-59400391 None:intergenic 20.0%
!! AAAGAAAAACTTACAGAATA+AGG - chr3.4:59402132-59402151 None:intergenic 20.0%
!! AAGAATTAAGGGAAAAAAAA+TGG - chr3.4:59399484-59399503 None:intergenic 20.0%
!! AAGATATAGAGAGAAAAAAA+AGG - chr3.4:59401561-59401580 None:intergenic 20.0%
!! AATTCTGTATAATAATAGAG+AGG + chr3.4:59400636-59400655 MS.gene04250:intron 20.0%
!! ATCTATGAAACAATAATTCA+AGG - chr3.4:59399633-59399652 None:intergenic 20.0%
!! ATTATTAAGATATGAAGGTA+TGG + chr3.4:59399256-59399275 MS.gene04250:intron 20.0%
!! CTAAAGTTAAAAAAAATCAC+AGG - chr3.4:59399400-59399419 None:intergenic 20.0%
!! TAATATGATACTAAACAGAA+AGG - chr3.4:59400589-59400608 None:intergenic 20.0%
!! TGAATAAAATGGTATAAGAT+TGG - chr3.4:59400060-59400079 None:intergenic 20.0%
!! TTTGAATATAATGTATGTGA+AGG - chr3.4:59398417-59398436 None:intergenic 20.0%
!!! ATTTCTGAAATTCATTACAA+TGG - chr3.4:59398209-59398228 None:intergenic 20.0%
!!! CTTATACCATTTTATTCATA+AGG + chr3.4:59400062-59400081 MS.gene04250:intron 20.0%
!!! GTAATATTTTGCAGAAAATA+TGG + chr3.4:59401444-59401463 MS.gene04250:intron 20.0%
!!! TTTCTGAAATTCATTACAAT+GGG - chr3.4:59398208-59398227 None:intergenic 20.0%
!!! TTTTTTCTTTTCTATTACGA+AGG + chr3.4:59400382-59400401 MS.gene04250:intron 20.0%
! AAACATTGTTGCAAATTTCT+TGG + chr3.4:59400507-59400526 MS.gene04250:intron 25.0%
! AAATATAGATCATGCAGTAA+AGG + chr3.4:59398225-59398244 MS.gene04250:CDS 25.0%
! AACATTGTTGCAAATTTCTT+GGG + chr3.4:59400508-59400527 MS.gene04250:intron 25.0%
! AAGACAATAGAAACAATTCT+GGG - chr3.4:59402673-59402692 None:intergenic 25.0%
! AATAACATGTATAACTGCTT+CGG - chr3.4:59399375-59399394 None:intergenic 25.0%
! AATAATATTTCTTCGGATCA+CGG - chr3.4:59403096-59403115 None:intergenic 25.0%
! AATAATTCAAGGCATATGTT+TGG - chr3.4:59399622-59399641 None:intergenic 25.0%
! AATATAGATCATGCAGTAAA+GGG + chr3.4:59398226-59398245 MS.gene04250:CDS 25.0%
! ACATAAGCTTATTAACCAAA+CGG - chr3.4:59400340-59400359 None:intergenic 25.0%
! ATCCTCAAAATGCAAAATTA+AGG + chr3.4:59401681-59401700 MS.gene04250:intron 25.0%
! ATGAGGAAATCTTATTCTTT+CGG + chr3.4:59401162-59401181 MS.gene04250:intron 25.0%
! ATGTCAATACAGATTTAAGA+AGG - chr3.4:59401596-59401615 None:intergenic 25.0%
! CAGCTGTAATAATATTTCTT+CGG - chr3.4:59403103-59403122 None:intergenic 25.0%
! CATCTGTAATTATATTGCTT+CGG - chr3.4:59398183-59398202 None:intergenic 25.0%
! CGTGATAGAAAATTAGAATT+GGG + chr3.4:59399176-59399195 MS.gene04250:CDS 25.0%
! CTAACAATATTAAATGCAGT+TGG + chr3.4:59402596-59402615 MS.gene04250:intron 25.0%
! CTAAGAAAGTTCCTTAAAAA+GGG + chr3.4:59402265-59402284 MS.gene04250:intron 25.0%
! GAATATGCATATGCTTATAA+TGG + chr3.4:59399547-59399566 MS.gene04250:intron 25.0%
! GAATTATTCATTATTAAGCC+AGG + chr3.4:59404071-59404090 MS.gene04250:CDS 25.0%
! GTATATATATATCTCGTATC+AGG - chr3.4:59401732-59401751 None:intergenic 25.0%
! TACTACAAGGATATAGTATA+TGG + chr3.4:59401533-59401552 MS.gene04250:CDS 25.0%
! TAGTTACAAACCATAATTCA+AGG - chr3.4:59399858-59399877 None:intergenic 25.0%
! TATTGTCATGAATTTCAGAA+TGG + chr3.4:59403127-59403146 MS.gene04250:CDS 25.0%
! TCATAGCTTGAATTCATAAA+TGG - chr3.4:59403799-59403818 None:intergenic 25.0%
! TCTAATGTTAACTTTGACAT+TGG + chr3.4:59401331-59401350 MS.gene04250:intron 25.0%
! TTAGATTAAGAACACAACAA+TGG - chr3.4:59402580-59402599 None:intergenic 25.0%
! TTGATTAGGGAAAATGAATA+GGG + chr3.4:59400727-59400746 MS.gene04250:intron 25.0%
! TTTGATTAGGGAAAATGAAT+AGG + chr3.4:59400726-59400745 MS.gene04250:intron 25.0%
!! ATATGTTTTATCTTTCACTC+TGG + chr3.4:59400293-59400312 MS.gene04250:intron 25.0%
!! ATCCTTAATTTTGCATTTTG+AGG - chr3.4:59401686-59401705 None:intergenic 25.0%
!! CAATTCTAATTTTCTATCAC+GGG - chr3.4:59399177-59399196 None:intergenic 25.0%
!! CCAATTCTAATTTTCTATCA+CGG - chr3.4:59399178-59399197 None:intergenic 25.0%
!! GAGGTGTATTTTGTATATAT+TGG - chr3.4:59401845-59401864 None:intergenic 25.0%
!! TAATCTTTCTTCTTAGTTTG+AGG - chr3.4:59402917-59402936 None:intergenic 25.0%
!! TGTACTTTTCTTATTCTTGA+TGG + chr3.4:59402324-59402343 MS.gene04250:intron 25.0%
!! TTAGAAAAGATCTCATCTTT+TGG - chr3.4:59402211-59402230 None:intergenic 25.0%
!! TTGTCAAACTAGTTTGATTA+GGG + chr3.4:59400714-59400733 MS.gene04250:intron 25.0%
!! TTTGTCAAACTAGTTTGATT+AGG + chr3.4:59400713-59400732 MS.gene04250:intron 25.0%
!!! ACATGTTTTAACCAAATGAA+GGG - chr3.4:59399597-59399616 None:intergenic 25.0%
!!! GATGGATTATTAAGATATGA+AGG + chr3.4:59399251-59399270 MS.gene04250:CDS 25.0%
!!! TACATGTTTTAACCAAATGA+AGG - chr3.4:59399598-59399617 None:intergenic 25.0%
!!! TTCATTTTCGCTTTTTATCA+AGG + chr3.4:59399894-59399913 MS.gene04250:intron 25.0%
AAAATGTCTTCATGGTTAGT+AGG - chr3.4:59403652-59403671 None:intergenic 30.0%
AAACAGAAAGGAATCAGATA+GGG - chr3.4:59400577-59400596 None:intergenic 30.0%
AAACTTGGAAAACAATGCAA+AGG - chr3.4:59402079-59402098 None:intergenic 30.0%
AAATAGCCTTCAAGGTTAAT+AGG - chr3.4:59398891-59398910 None:intergenic 30.0%
AATCAAAGTTACTCTACATG+AGG - chr3.4:59401864-59401883 None:intergenic 30.0%
AATTATATTGCTTCGGATCA+CGG - chr3.4:59398176-59398195 None:intergenic 30.0%
ACGCGAAAAGAAAATGATTT+AGG - chr3.4:59400263-59400282 None:intergenic 30.0%
AGAACAAAGCTTTGCAATAA+TGG + chr3.4:59403223-59403242 MS.gene04250:CDS 30.0%
ATATAGATCATGCAGTAAAG+GGG + chr3.4:59398227-59398246 MS.gene04250:CDS 30.0%
ATGCTTTGTCATATCAATGT+TGG + chr3.4:59402538-59402557 MS.gene04250:intron 30.0%
ATTATTAAGCCAGGAATCTT+GGG + chr3.4:59404080-59404099 MS.gene04250:CDS 30.0%
ATTTCATTGAGTTTATCACC+GGG - chr3.4:59403973-59403992 None:intergenic 30.0%
CAACTTCAACATTACAATGT+TGG - chr3.4:59399308-59399327 None:intergenic 30.0%
CAAGACAATAGAAACAATTC+TGG - chr3.4:59402674-59402693 None:intergenic 30.0%
CAATCACATTTGAATTGGTT+AGG - chr3.4:59403753-59403772 None:intergenic 30.0%
CAATGATAGAAGAGTCAAAT+AGG + chr3.4:59403611-59403630 MS.gene04250:CDS 30.0%
CATTTCATTGAGTTTATCAC+CGG - chr3.4:59403974-59403993 None:intergenic 30.0%
CCGTGATAGAAAATTAGAAT+TGG + chr3.4:59399175-59399194 MS.gene04250:CDS 30.0%
CTAACCAATTCAAATGTGAT+TGG + chr3.4:59403751-59403770 MS.gene04250:CDS 30.0%
CTATTTGACTCTTCTATCAT+TGG - chr3.4:59403613-59403632 None:intergenic 30.0%
GAAGATTGTATCAAAGAAGA+CGG - chr3.4:59403400-59403419 None:intergenic 30.0%
GATACATGTCAATATTCACA+TGG + chr3.4:59403166-59403185 MS.gene04250:CDS 30.0%
GCTAAGAAAGTTCCTTAAAA+AGG + chr3.4:59402264-59402283 MS.gene04250:intron 30.0%
GGTGAATTTGAATTTGATGT+AGG - chr3.4:59403340-59403359 None:intergenic 30.0%
GTAAGGAATCATACAGTAAA+GGG - chr3.4:59400953-59400972 None:intergenic 30.0%
GTACATTCAAAAAACGAGAA+TGG - chr3.4:59399970-59399989 None:intergenic 30.0%
GTGATGAACTCAATAAAATG+AGG + chr3.4:59401145-59401164 MS.gene04250:intron 30.0%
TAAACAGAAAGGAATCAGAT+AGG - chr3.4:59400578-59400597 None:intergenic 30.0%
TAACCACATTAGAATTGGTA+AGG - chr3.4:59398992-59399011 None:intergenic 30.0%
TAAGGAATCATACAGTAAAG+GGG - chr3.4:59400952-59400971 None:intergenic 30.0%
TACTAGCAATGTTTCAACAA+TGG + chr3.4:59404026-59404045 MS.gene04250:CDS 30.0%
TATATCCTATCCTTTATGTC+AGG - chr3.4:59402959-59402978 None:intergenic 30.0%
TATGATATCAATCTGTTGCT+CGG - chr3.4:59398585-59398604 None:intergenic 30.0%
TATGGTTAATGAACTTGTGA+AGG + chr3.4:59399274-59399293 MS.gene04250:intron 30.0%
TGATCAATTGTTGAATGAAG+TGG - chr3.4:59400150-59400169 None:intergenic 30.0%
TGATCCAATCACATTTGAAT+TGG - chr3.4:59403758-59403777 None:intergenic 30.0%
TGATCTAACCACATTAGAAT+TGG - chr3.4:59398997-59399016 None:intergenic 30.0%
TGATGTATACATTCAAAGTG+CGG + chr3.4:59398085-59398104 MS.gene04250:CDS 30.0%
TGTGTTTATTTCCAAAAGAG+TGG - chr3.4:59399941-59399960 None:intergenic 30.0%
TTACAAAATCATCAATCACC+AGG - chr3.4:59402819-59402838 None:intergenic 30.0%
TTTGAATTGGAAAGATCAGA+AGG - chr3.4:59401019-59401038 None:intergenic 30.0%
TTTGAATTTGATGTAGGTGA+AGG - chr3.4:59403334-59403353 None:intergenic 30.0%
! AAGGATATATCTGAAGTTGT+TGG - chr3.4:59398965-59398984 None:intergenic 30.0%
! AATATTTGTTGCCAATTTCC+TGG + chr3.4:59402798-59402817 MS.gene04250:intron 30.0%
! ACTAACCATGAAGACATTTT+CGG + chr3.4:59403652-59403671 MS.gene04250:CDS 30.0%
! ATTAACCTTGAAGGCTATTT+TGG + chr3.4:59398891-59398910 MS.gene04250:CDS 30.0%
! GAGATTACAATGTGTTTTCT+TGG + chr3.4:59402392-59402411 MS.gene04250:intron 30.0%
! GTGTCGATAATACAAAAGTT+AGG + chr3.4:59401471-59401490 MS.gene04250:CDS 30.0%
!! AACAACTTGTTACTACTACA+AGG + chr3.4:59401520-59401539 MS.gene04250:CDS 30.0%
!! GTCTTGCTATTTATGTACTA+AGG + chr3.4:59402688-59402707 MS.gene04250:intron 30.0%
!! TACATCAAATGTTGATGATC+AGG + chr3.4:59399229-59399248 MS.gene04250:CDS 30.0%
!! TTAACCTTGAAGGCTATTTT+GGG + chr3.4:59398892-59398911 MS.gene04250:CDS 30.0%
!!! CAGTGTCTTTTGTTGTTTTA+CGG - chr3.4:59402037-59402056 None:intergenic 30.0%
!!! TACGCTAGTTTACTGATTTT+GGG + chr3.4:59399987-59400006 MS.gene04250:intron 30.0%
!!! TTTGTTGTTTTACGGTTTTG+AGG - chr3.4:59402029-59402048 None:intergenic 30.0%
AAACAATGCAAAGGAAGAAG+TGG - chr3.4:59402070-59402089 None:intergenic 35.0%
AAACATTGCTAGTATCGTCT+AGG - chr3.4:59404020-59404039 None:intergenic 35.0%
AAACCAGAAGCTACTATTTG+CGG - chr3.4:59399774-59399793 None:intergenic 35.0%
AAAGGAATCAGATAGGGAAA+TGG - chr3.4:59400571-59400590 None:intergenic 35.0%
AATCAGATAGGGAAATGGAA+AGG - chr3.4:59400566-59400585 None:intergenic 35.0%
ACTGTAGTACTAATCTGAGT+CGG - chr3.4:59398726-59398745 None:intergenic 35.0%
ACTGTAGTATGAATCTGAGT+CGG - chr3.4:59403487-59403506 None:intergenic 35.0%
AGAACAACGCTTTGCAATAA+TGG + chr3.4:59398306-59398325 MS.gene04250:CDS 35.0%
AGATTGATATCATATCCTGC+AGG + chr3.4:59398591-59398610 MS.gene04250:CDS 35.0%
AGCAAAAAAGCAAACTCTTC+TGG + chr3.4:59400836-59400855 MS.gene04250:intron 35.0%
ATAGAGAGAGTTAGTTGAGT+TGG - chr3.4:59401900-59401919 None:intergenic 35.0%
ATCATCAATCACCAGGAAAT+TGG - chr3.4:59402812-59402831 None:intergenic 35.0%
ATCCTGGTCAATATAGAACA+AGG - chr3.4:59400887-59400906 None:intergenic 35.0%
ATTGCGGTTATGTTCATGAT+TGG + chr3.4:59398109-59398128 MS.gene04250:CDS 35.0%
CAAACATACGCCTTGAATTA+TGG + chr3.4:59399845-59399864 MS.gene04250:intron 35.0%
CATAATTCAAGGCGTATGTT+TGG - chr3.4:59399847-59399866 None:intergenic 35.0%
CATTATTAAGCCAGGAATCT+TGG + chr3.4:59404079-59404098 MS.gene04250:CDS 35.0%
CGTAAGGAATCATACAGTAA+AGG - chr3.4:59400954-59400973 None:intergenic 35.0%
CTAACTCTCTCTATGAAATC+TGG + chr3.4:59401906-59401925 MS.gene04250:intron 35.0%
CTTTCCAATTCAAATGTGAG+TGG + chr3.4:59401025-59401044 MS.gene04250:intron 35.0%
GAATCCATTGAAGAAACACT+AGG - chr3.4:59403361-59403380 None:intergenic 35.0%
GATGAATGTCAATATGCACA+TGG + chr3.4:59398249-59398268 MS.gene04250:CDS 35.0%
GTGAATCCTATTAACCTTGA+AGG + chr3.4:59398882-59398901 MS.gene04250:CDS 35.0%
TAAACTTCAGTGCCATAATC+CGG + chr3.4:59399332-59399351 MS.gene04250:intron 35.0%
TATGATTCCTTACGAAACTC+AGG + chr3.4:59400960-59400979 MS.gene04250:intron 35.0%
TATTGCTCTCAATAAAGCTC+TGG - chr3.4:59401090-59401109 None:intergenic 35.0%
TCAACTACAATGCCATAATC+CGG + chr3.4:59404170-59404189 MS.gene04250:CDS 35.0%
TCACCTTACCAATTCTAATG+TGG + chr3.4:59398986-59399005 MS.gene04250:CDS 35.0%
TCTAAACCTTCACCAAGTTT+CGG + chr3.4:59402098-59402117 MS.gene04250:CDS 35.0%
TGATCCACTCACATTTGAAT+TGG - chr3.4:59401032-59401051 None:intergenic 35.0%
TGCGTGTATGTTTGAATATG+CGG + chr3.4:59399703-59399722 MS.gene04250:intron 35.0%
TGCTAGAGATAATACTCATC+TGG + chr3.4:59398791-59398810 MS.gene04250:CDS 35.0%
TGTATGTTTGAATATGCGGT+GGG + chr3.4:59399707-59399726 MS.gene04250:intron 35.0%
TGTTGATCAGATTCCAATGT+TGG - chr3.4:59404132-59404151 None:intergenic 35.0%
TTAGAATTGGTAAGGTGAGA+AGG - chr3.4:59398984-59399003 None:intergenic 35.0%
TTCACCTAGTGTTTCTTCAA+TGG + chr3.4:59403354-59403373 MS.gene04250:CDS 35.0%
TTCAGCCAAAGGTTATACTA+TGG + chr3.4:59400792-59400811 MS.gene04250:CDS 35.0%
TTCGACCATAGTATAACCTT+TGG - chr3.4:59400800-59400819 None:intergenic 35.0%
TTGAATTGGTTAGGTCAGAA+GGG - chr3.4:59403744-59403763 None:intergenic 35.0%
TTGGTGAAGGTTTAGAAACT+TGG - chr3.4:59402094-59402113 None:intergenic 35.0%
TTTCATTGAGTTTATCACCG+GGG - chr3.4:59403972-59403991 None:intergenic 35.0%
TTTGAATTGGTTAGGTCAGA+AGG - chr3.4:59403745-59403764 None:intergenic 35.0%
TTTGCCAGAGAAATTGTACA+TGG + chr3.4:59401284-59401303 MS.gene04250:CDS 35.0%
! AGCATCTGACAAACTGATTT+AGG - chr3.4:59400206-59400225 None:intergenic 35.0%
! AGGGTGTGTTCTGAATTATT+TGG + chr3.4:59402284-59402303 MS.gene04250:intron 35.0%
! ATGACGACGTCTTATTCATT+CGG + chr3.4:59403991-59404010 MS.gene04250:CDS 35.0%
! CTAACCATGAAGACATTTTC+GGG + chr3.4:59403653-59403672 MS.gene04250:CDS 35.0%
! GAGATTGCAATGTGTTTTCT+TGG + chr3.4:59402239-59402258 MS.gene04250:intron 35.0%
! GGGTGTGTTCTGAATTATTT+GGG + chr3.4:59402285-59402304 MS.gene04250:intron 35.0%
! GTATCAAACTACCACTCTTT+TGG + chr3.4:59399927-59399946 MS.gene04250:intron 35.0%
! GTGATATTTCGCTGCAATTT+TGG - chr3.4:59399738-59399757 None:intergenic 35.0%
! TTCAGAACACACCCTTTTTA+AGG - chr3.4:59402279-59402298 None:intergenic 35.0%
!! AGCTTTATTGAGAGCAATAG+CGG + chr3.4:59401091-59401110 MS.gene04250:CDS 35.0%
!! TCAAATGTTGATGATCAGGA+TGG + chr3.4:59399233-59399252 MS.gene04250:CDS 35.0%
!!! ATAGTAGCTTCTGGTTTTCA+TGG + chr3.4:59399777-59399796 MS.gene04250:intron 35.0%
!!! CTCTCAAGAAACACTTTTTC+AGG - chr3.4:59402432-59402451 None:intergenic 35.0%
!!! GTACGCTAGTTTACTGATTT+TGG + chr3.4:59399986-59400005 MS.gene04250:intron 35.0%
AAATGTCCCTTTGCTCATCA+TGG + chr3.4:59398135-59398154 MS.gene04250:CDS 40.0%
AACTGGTACTAGCATTCAAC+GGG - chr3.4:59398386-59398405 None:intergenic 40.0%
AACTGGTATAAGCCTTCAAC+AGG - chr3.4:59403303-59403322 None:intergenic 40.0%
AAGCAGAAACAACATGTAGG+TGG + chr3.4:59402890-59402909 MS.gene04250:CDS 40.0%
AATCAGTTTGTCAGATGCTG+AGG + chr3.4:59400207-59400226 MS.gene04250:intron 40.0%
AATGCAACTGAGGTAGAATC+TGG - chr3.4:59399122-59399141 None:intergenic 40.0%
AATGTCCCTTTGCTCATCAT+GGG + chr3.4:59398136-59398155 MS.gene04250:CDS 40.0%
AATGTCCCTTTGCTCATTCT+GGG + chr3.4:59403056-59403075 MS.gene04250:CDS 40.0%
AATTTCCCCAGAATGAGCAA+AGG - chr3.4:59403065-59403084 None:intergenic 40.0%
ACAATCTTCGAAAGACATGC+TGG + chr3.4:59403684-59403703 MS.gene04250:CDS 40.0%
ACTGTAGTACGATTCTGAGT+CGG - chr3.4:59398546-59398565 None:intergenic 40.0%
AGAATGGATCATGCAGTAGA+GGG + chr3.4:59403143-59403162 MS.gene04250:CDS 40.0%
AGATTCTACCTCAGTTGCAT+TGG + chr3.4:59399121-59399140 MS.gene04250:CDS 40.0%
ATGAAAGCTCACCCTTCATT+TGG + chr3.4:59399583-59399602 MS.gene04250:intron 40.0%
ATGACCCAAAATAGCCTTCA+AGG - chr3.4:59398899-59398918 None:intergenic 40.0%
ATGACCCGAAAATGTCTTCA+TGG - chr3.4:59403660-59403679 None:intergenic 40.0%
ATGCCGAATCCAATGATAGA+AGG + chr3.4:59398840-59398859 MS.gene04250:CDS 40.0%
ATTTCCCCAGAATGAGCAAA+GGG - chr3.4:59403064-59403083 None:intergenic 40.0%
CAAAGTGAAGCTTTGCTCAT+GGG + chr3.4:59403009-59403028 MS.gene04250:CDS 40.0%
CAATGATAGAAGGGTCAAGT+AGG + chr3.4:59398850-59398869 MS.gene04250:CDS 40.0%
CAATTCTTTGCTTCCATCTG+CGG + chr3.4:59401123-59401142 MS.gene04250:intron 40.0%
CACTAGCAACACTTCAACAA+TGG + chr3.4:59399202-59399221 MS.gene04250:CDS 40.0%
CCAATCTACCTTTCTATCAG+TGG - chr3.4:59403944-59403963 None:intergenic 40.0%
CCACTGATAGAAAGGTAGAT+TGG + chr3.4:59403941-59403960 MS.gene04250:CDS 40.0%
CCTATCCTTTATGTCAGGTA+GGG - chr3.4:59402954-59402973 None:intergenic 40.0%
CTACTTGACCCTTCTATCAT+TGG - chr3.4:59398852-59398871 None:intergenic 40.0%
CTTTATTGAGAGCAATAGCG+GGG + chr3.4:59401093-59401112 MS.gene04250:CDS 40.0%
GAACTGGTACTAGCATTCAA+CGG - chr3.4:59398387-59398406 None:intergenic 40.0%
GAAGGATGATGATCCAACAT+TGG + chr3.4:59404116-59404135 MS.gene04250:CDS 40.0%
GACACAAATCAGCAACTTTG+GGG + chr3.4:59400989-59401008 MS.gene04250:intron 40.0%
GACCCTTCTATCATTGGATT+CGG - chr3.4:59398846-59398865 None:intergenic 40.0%
GAGAAGCAGAAACAACATGT+AGG + chr3.4:59402887-59402906 MS.gene04250:CDS 40.0%
GAGTGGATCACAACAATTCA+GGG + chr3.4:59401042-59401061 MS.gene04250:intron 40.0%
GATGCTTGTGAATATGCACA+CGG - chr3.4:59400930-59400949 None:intergenic 40.0%
GCAAGTAACTACTCACAAGT+TGG - chr3.4:59400116-59400135 None:intergenic 40.0%
GCAGGATATGATATTGCAGA+CGG - chr3.4:59398501-59398520 None:intergenic 40.0%
GCAGTATTCAATCTGTTGCT+CGG - chr3.4:59398765-59398784 None:intergenic 40.0%
GGACACAAATCAGCAACTTT+GGG + chr3.4:59400988-59401007 MS.gene04250:intron 40.0%
GGTTAGATCACAACAGTTCA+GGG + chr3.4:59399007-59399026 MS.gene04250:CDS 40.0%
GTGTATGTTTGAATATGCGG+TGG + chr3.4:59399706-59399725 MS.gene04250:intron 40.0%
GTTAGATCACAACAGTTCAG+GGG + chr3.4:59399008-59399027 MS.gene04250:CDS 40.0%
TATAACTGCTTCGGCTAAGA+TGG - chr3.4:59399366-59399385 None:intergenic 40.0%
TCAAAGTGAAGCTTTGCTCA+TGG + chr3.4:59403008-59403027 MS.gene04250:CDS 40.0%
TCCTATCCTTTATGTCAGGT+AGG - chr3.4:59402955-59402974 None:intergenic 40.0%
TCTGCAATATCATATCCTGC+AGG + chr3.4:59398501-59398520 MS.gene04250:CDS 40.0%
TGAGTGGATCACAACAATTC+AGG + chr3.4:59401041-59401060 MS.gene04250:intron 40.0%
TGCCGAATCCAATGATAGAA+GGG + chr3.4:59398841-59398860 MS.gene04250:CDS 40.0%
TGCTCCATGTACAATTTCTC+TGG - chr3.4:59401291-59401310 None:intergenic 40.0%
TGGTTAGATCACAACAGTTC+AGG + chr3.4:59399006-59399025 MS.gene04250:CDS 40.0%
TTGGTACTCCACTGATAGAA+AGG + chr3.4:59403933-59403952 MS.gene04250:CDS 40.0%
! CTCTCTAGAAGACAGAAAGA+AGG + chr3.4:59402492-59402511 MS.gene04250:intron 40.0%
! GCAGTTTTCAATCTGTTGCT+TGG - chr3.4:59403526-59403545 None:intergenic 40.0%
! GCTATGATGCCATTTCGAAT+CGG + chr3.4:59403812-59403831 MS.gene04250:CDS 40.0%
! GCTTTATTGAGAGCAATAGC+GGG + chr3.4:59401092-59401111 MS.gene04250:CDS 40.0%
! TTGCTTGAGTTTTCAGCCAA+AGG + chr3.4:59400781-59400800 MS.gene04250:CDS 40.0%
!! AGCCTTGTTCTATATTGACC+AGG + chr3.4:59400882-59400901 MS.gene04250:intron 40.0%
!! AGCTTTGTTCTGTATCGACT+AGG - chr3.4:59403214-59403233 None:intergenic 40.0%
!! GACTCTTCTATCATTGGCTT+CGG - chr3.4:59403607-59403626 None:intergenic 40.0%
!! GTATACATTGCAGAAGCACT+TGG - chr3.4:59398444-59398463 None:intergenic 40.0%
!!! TACAGCTTGTTTTTGCCGTT+TGG + chr3.4:59400322-59400341 MS.gene04250:intron 40.0%
!!! TCACATGGTGTTTTTGAGTC+CGG + chr3.4:59403181-59403200 MS.gene04250:CDS 40.0%
AAGCCGCAAATAGTAGCTTC+TGG + chr3.4:59399768-59399787 MS.gene04250:intron 45.0%
AAGGAAACTGCACCGAAACT+TGG - chr3.4:59402113-59402132 None:intergenic 45.0%
AAGGGTAGTCACACAGTTCT+TGG - chr3.4:59403726-59403745 None:intergenic 45.0%
AATGCAACTGAGGTAGCAGA+AGG - chr3.4:59403889-59403908 None:intergenic 45.0%
AATGGATCAACGCTGAGTTG+TGG - chr3.4:59403781-59403800 None:intergenic 45.0%
ACAGAACCAAGATTGCCTCA+AGG + chr3.4:59402847-59402866 MS.gene04250:intron 45.0%
ACATTCAAAGTGCGGCATTG+CGG + chr3.4:59398093-59398112 MS.gene04250:CDS 45.0%
AGGTGAAGGAAAAGCAGAAC+TGG - chr3.4:59403320-59403339 None:intergenic 45.0%
ATACTATGGTCGAAGCTCCT+CGG + chr3.4:59400806-59400825 MS.gene04250:CDS 45.0%
ATCCTGCAGAAAGAACCATG+TGG + chr3.4:59398694-59398713 MS.gene04250:CDS 45.0%
ATGGAACATCGTCCGGATTA+TGG - chr3.4:59399347-59399366 None:intergenic 45.0%
ATGTCCCTTTGCTCATCATG+GGG + chr3.4:59398137-59398156 MS.gene04250:CDS 45.0%
ATGTCCCTTTGCTCATTCTG+GGG + chr3.4:59403057-59403076 MS.gene04250:CDS 45.0%
ATGTGAAGGCAAAGCAGAAC+TGG - chr3.4:59398403-59398422 None:intergenic 45.0%
ATGTTTGAATATGCGGTGGG+AGG + chr3.4:59399710-59399729 MS.gene04250:intron 45.0%
ATTGAGTTCATCACCGCAGA+TGG - chr3.4:59401139-59401158 None:intergenic 45.0%
CAAGATATGATGCCCTTTCG+AGG + chr3.4:59399048-59399067 MS.gene04250:CDS 45.0%
CAAGGGAGAAGGTTTCGAAA+TGG + chr3.4:59402865-59402884 MS.gene04250:CDS 45.0%
CAATCAGAGAAGGCAGTAGA+AGG - chr3.4:59403916-59403935 None:intergenic 45.0%
CAGAACCAAGATTGCCTCAA+GGG + chr3.4:59402848-59402867 MS.gene04250:intron 45.0%
CAGAATGGATCATGCAGTAG+AGG + chr3.4:59403142-59403161 MS.gene04250:CDS 45.0%
CCAATGCTATAATCCAGACG+CGG + chr3.4:59401254-59401273 MS.gene04250:CDS 45.0%
CCCTACCTGACATAAAGGAT+AGG + chr3.4:59402951-59402970 MS.gene04250:CDS 45.0%
CCTTACGAAACTCAGGACAA+GGG + chr3.4:59400967-59400986 MS.gene04250:intron 45.0%
CTTCTACTGCCTTCTCTGAT+TGG + chr3.4:59403914-59403933 MS.gene04250:CDS 45.0%
CTTCTTTGCTCACAAACCTG+AGG + chr3.4:59398353-59398372 MS.gene04250:CDS 45.0%
CTTTCCCCATGATGAGCAAA+GGG - chr3.4:59398144-59398163 None:intergenic 45.0%
GAAGAGTCAAATAGGCTTGC+AGG + chr3.4:59403619-59403638 MS.gene04250:CDS 45.0%
GAATGGATCATGCAGTAGAG+GGG + chr3.4:59403144-59403163 MS.gene04250:CDS 45.0%
GAATGTCCCTTTGCTCATTC+TGG + chr3.4:59403055-59403074 MS.gene04250:CDS 45.0%
GCAAACTCTTCTGGTGCAAT+AGG + chr3.4:59400845-59400864 MS.gene04250:intron 45.0%
GGGACACAAATCAGCAACTT+TGG + chr3.4:59400987-59401006 MS.gene04250:intron 45.0%
GGGATAATACTCCTCATGAG+TGG + chr3.4:59403029-59403048 MS.gene04250:CDS 45.0%
GGGTGACACTGCAGAAAATA+TGG + chr3.4:59399437-59399456 MS.gene04250:intron 45.0%
GTACATGGAGCATAAGCAGA+AGG + chr3.4:59401299-59401318 MS.gene04250:CDS 45.0%
GTACTAATCTGAGTCGGAGT+CGG - chr3.4:59398720-59398739 None:intergenic 45.0%
GTCATGTGCAACGATACGAA+TGG + chr3.4:59400685-59400704 MS.gene04250:intron 45.0%
TCCTTACGAAACTCAGGACA+AGG + chr3.4:59400966-59400985 MS.gene04250:intron 45.0%
TCGATCCCTACCTGACATAA+AGG + chr3.4:59402946-59402965 MS.gene04250:CDS 45.0%
TGAGGTAGAATCTGGAAGCT+TGG - chr3.4:59399114-59399133 None:intergenic 45.0%
TGCTGCTCTCGATAAAGTTC+TGG - chr3.4:59399079-59399098 None:intergenic 45.0%
TGGATCCTTTGTGCATCTCT+AGG + chr3.4:59401351-59401370 MS.gene04250:intron 45.0%
TTTCGAAACCTTCTCCCTTG+AGG - chr3.4:59402865-59402884 None:intergenic 45.0%
! ACTAGCCTAGAGATGCACAA+AGG - chr3.4:59401359-59401378 None:intergenic 45.0%
! AGAAGGCTCTAATGCAACTG+AGG - chr3.4:59403899-59403918 None:intergenic 45.0%
! AGTGGAGTACCAATCAGAGA+AGG - chr3.4:59403926-59403945 None:intergenic 45.0%
! CTCATTCTGGGGAAATTGCA+AGG + chr3.4:59403068-59403087 MS.gene04250:CDS 45.0%
! TTGCTTGGCATCTGAAGTGT+GGG - chr3.4:59403511-59403530 None:intergenic 45.0%
!! AAGGTAGATTGGTCTATCCC+CGG + chr3.4:59403952-59403971 MS.gene04250:CDS 45.0%
!! AGCGTTGTTCTGTATCGACT+AGG - chr3.4:59398297-59398316 None:intergenic 45.0%
!! CTATCATTGGCTTCGGCATA+TGG - chr3.4:59403600-59403619 None:intergenic 45.0%
!! TCTGGATTATAGCATTGGCG+TGG - chr3.4:59401252-59401271 None:intergenic 45.0%
!! TTGGGTTAGCTCACTTGTGA+AGG + chr3.4:59404098-59404117 MS.gene04250:CDS 45.0%
!!! CACACGGTATTTTCGAGTTC+TGG - chr3.4:59400914-59400933 None:intergenic 45.0%
!!! CACATGGTGTTTTTGAGTCC+GGG + chr3.4:59403182-59403201 MS.gene04250:CDS 45.0%
!!! CTTTTTTGCTCACAAACCCG+AGG - chr3.4:59400826-59400845 None:intergenic 45.0%
!!! GGGTTGATGCCTTTTCGAAA+CGG + chr3.4:59401062-59401081 MS.gene04250:intron 45.0%
AATCTCCGACAGCAATGCAG+GGG + chr3.4:59398925-59398944 MS.gene04250:CDS 50.0%
AATCTGTTGCTCGGCATCTG+AGG - chr3.4:59398576-59398595 None:intergenic 50.0%
AATCTTCGTCGACACTGCCT+TGG + chr3.4:59403413-59403432 MS.gene04250:CDS 50.0%
ACAATCTCCGACAGCAATGC+AGG + chr3.4:59398923-59398942 MS.gene04250:CDS 50.0%
ACGGAACATCGTCCGGATTA+TGG - chr3.4:59404185-59404204 None:intergenic 50.0%
ATCCTGCAGGAAGAACCATG+TGG + chr3.4:59398514-59398533 MS.gene04250:CDS 50.0%
CAAGATTGCCTCAAGGGAGA+AGG + chr3.4:59402854-59402873 MS.gene04250:CDS 50.0%
CAATCTCCGACAGCAATGCA+GGG + chr3.4:59398924-59398943 MS.gene04250:CDS 50.0%
CACATGGTGTTCTTGAGTCC+GGG + chr3.4:59398265-59398284 MS.gene04250:CDS 50.0%
CATGGAGCATAAGCAGAAGG+TGG + chr3.4:59401302-59401321 MS.gene04250:intron 50.0%
CCCTTGTCCTGAGTTTCGTA+AGG - chr3.4:59400970-59400989 None:intergenic 50.0%
CCGCGTCTGGATTATAGCAT+TGG - chr3.4:59401257-59401276 None:intergenic 50.0%
CTTCTCCCTTGAGGCAATCT+TGG - chr3.4:59402856-59402875 None:intergenic 50.0%
CTTCTTTGCTCACAAACCCG+AGG + chr3.4:59403270-59403289 MS.gene04250:CDS 50.0%
CTTTATCGAGAGCAGCAGCA+TGG + chr3.4:59399082-59399101 MS.gene04250:CDS 50.0%
GATATTGCAGACGGTGTCCA+AGG - chr3.4:59398492-59398511 None:intergenic 50.0%
GCACATGGTGTTCTTGAGTC+CGG + chr3.4:59398264-59398283 MS.gene04250:CDS 50.0%
GCAGGAGATGATGTTGCAGA+CGG - chr3.4:59403442-59403461 None:intergenic 50.0%
GCTTTCCCCATGATGAGCAA+AGG - chr3.4:59398145-59398164 None:intergenic 50.0%
GGACATTCTGTCCACTCATG+AGG - chr3.4:59403043-59403062 None:intergenic 50.0%
GGCTAAGATGGAACATCGTC+CGG - chr3.4:59399354-59399373 None:intergenic 50.0%
GTACGATTCTGAGTCGGAGT+CGG - chr3.4:59398540-59398559 None:intergenic 50.0%
GTGAGCTAACCCAAGATTCC+TGG - chr3.4:59404092-59404111 None:intergenic 50.0%
TACTATGGTCGAAGCTCCTC+GGG + chr3.4:59400807-59400826 MS.gene04250:CDS 50.0%
TCTGCAACATCATCTCCTGC+AGG + chr3.4:59403442-59403461 MS.gene04250:CDS 50.0%
TGAAGGCTCCAATGCAACTG+AGG - chr3.4:59399132-59399151 None:intergenic 50.0%
TGATACCCCTGCATTGCTGT+CGG - chr3.4:59398933-59398952 None:intergenic 50.0%
TGCTGTGCTCGATAAAGCTC+TGG - chr3.4:59403840-59403859 None:intergenic 50.0%
TGGCGTGGATCTTCAACATC+TGG - chr3.4:59401237-59401256 None:intergenic 50.0%
! ACTGCACCGAAACTTGGTGA+AGG - chr3.4:59402107-59402126 None:intergenic 50.0%
! GTTGCTTGGCATCTGAAGTG+TGG - chr3.4:59403512-59403531 None:intergenic 50.0%
! TGCTTGGCATCTGAAGTGTG+GGG - chr3.4:59403510-59403529 None:intergenic 50.0%
!! TTCGAGTTCTGGCTTCATCC+TGG - chr3.4:59400903-59400922 None:intergenic 50.0%
ATTGCAGACGGTGTCCAAGG+CGG - chr3.4:59398489-59398508 None:intergenic 55.0%
CAACCGGAGAAGGCAGTTGA+AGG - chr3.4:59399149-59399168 None:intergenic 55.0%
CAGTGACTAGTGCAGCAGCT+TGG + chr3.4:59401986-59402005 MS.gene04250:intron 55.0%
CTTCAACAGGCGAAGCTCCT+CGG - chr3.4:59403290-59403309 None:intergenic 55.0%
CTTCAACTGCCTTCTCCGGT+TGG + chr3.4:59399147-59399166 MS.gene04250:CDS 55.0%
CTTTATCGAGCACAGCAGCG+TGG + chr3.4:59403843-59403862 MS.gene04250:CDS 55.0%
GAAGGGTCAAGTAGGCTTGC+TGG + chr3.4:59398858-59398877 MS.gene04250:CDS 55.0%
GACTAGTGCAGCAGCTTGGT+GGG + chr3.4:59401990-59402009 MS.gene04250:CDS 55.0%
GAGCCTTCAACTGCCTTCTC+CGG + chr3.4:59399143-59399162 MS.gene04250:CDS 55.0%
GATGTTGCAGACGGAGTCCA+AGG - chr3.4:59403433-59403452 None:intergenic 55.0%
GCAACATCATCTCCTGCAGG+AGG + chr3.4:59403445-59403464 MS.gene04250:CDS 55.0%
GGCCACATGGTTCTTTCTGC+AGG - chr3.4:59398699-59398718 None:intergenic 55.0%
TGACTAGTGCAGCAGCTTGG+TGG + chr3.4:59401989-59402008 MS.gene04250:CDS 55.0%
TTCAACAGGCGAAGCTCCTC+GGG - chr3.4:59403289-59403308 None:intergenic 55.0%
TTCAACGGGCGAAGTTCCTC+AGG - chr3.4:59398372-59398391 None:intergenic 55.0%
! GCTCTGGCTCCGATTCGAAA+TGG - chr3.4:59403824-59403843 None:intergenic 55.0%
! GCTCTGGCTCCGTTTCGAAA+AGG - chr3.4:59401074-59401093 None:intergenic 55.0%
! TGCTCGGCATCTGAGGTGTA+GGG - chr3.4:59398569-59398588 None:intergenic 55.0%
! TTGCTCGGCATCTGAGGTGT+AGG - chr3.4:59398570-59398589 None:intergenic 55.0%
!! AGTTCTGGCTCTGCCTCGAA+AGG - chr3.4:59399064-59399083 None:intergenic 55.0%
!! GTATCGACTAGGATGCAGCC+CGG - chr3.4:59398286-59398305 None:intergenic 55.0%
!! GTTCTGGCTCTGCCTCGAAA+GGG - chr3.4:59399063-59399082 None:intergenic 55.0%
CGAGGAGCTTCGCCTGTTGA+AGG + chr3.4:59403288-59403307 MS.gene04250:CDS 60.0%
CTCCTGCAGGAGGAACCATG+TGG + chr3.4:59403455-59403474 MS.gene04250:CDS 60.0%
CTGAGTCGGAGTCTGCCACA+TGG - chr3.4:59403473-59403492 None:intergenic 60.0%
GGCAAAGACGAAACCGCGTC+TGG - chr3.4:59401270-59401289 None:intergenic 60.0%
GGCCACATGGTTCTTCCTGC+AGG - chr3.4:59398519-59398538 None:intergenic 60.0%
TGCCACATGGTTCCTCCTGC+AGG - chr3.4:59403460-59403479 None:intergenic 60.0%
TGCCTTCATCGATGCCGCCT+TGG + chr3.4:59398472-59398491 MS.gene04250:CDS 60.0%
TGCTGCAGTCGCACGACTGA+TGG - chr3.4:59399811-59399830 None:intergenic 60.0%
!! GTCCAAGGCGGCATCGATGA+AGG - chr3.4:59398477-59398496 None:intergenic 60.0%
!! TCTATCACGGGAGCGCCAAC+CGG - chr3.4:59399165-59399184 None:intergenic 60.0%
CTGAGTCGGAGTCGGCCACA+TGG - chr3.4:59398532-59398551 None:intergenic 65.0%
!! ACGGGAGCGCCAACCGGAGA+AGG - chr3.4:59399159-59399178 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 59398054 59404209 59398054 ID=MS.gene04250
chr3.4 mRNA 59398054 59404209 59398054 ID=MS.gene04250.t1;Parent=MS.gene04250
chr3.4 exon 59398054 59399272 59398054 ID=MS.gene04250.t1.exon1;Parent=MS.gene04250.t1
chr3.4 CDS 59398054 59399272 59398054 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59400779 59400828 59400779 ID=MS.gene04250.t1.exon2;Parent=MS.gene04250.t1
chr3.4 CDS 59400779 59400828 59400779 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59400904 59400958 59400904 ID=MS.gene04250.t1.exon3;Parent=MS.gene04250.t1
chr3.4 CDS 59400904 59400958 59400904 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59401063 59401114 59401063 ID=MS.gene04250.t1.exon4;Parent=MS.gene04250.t1
chr3.4 CDS 59401063 59401114 59401063 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59401197 59401320 59401197 ID=MS.gene04250.t1.exon5;Parent=MS.gene04250.t1
chr3.4 CDS 59401197 59401320 59401197 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59401458 59401554 59401458 ID=MS.gene04250.t1.exon6;Parent=MS.gene04250.t1
chr3.4 CDS 59401458 59401554 59401458 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59401989 59402137 59401989 ID=MS.gene04250.t1.exon7;Parent=MS.gene04250.t1
chr3.4 CDS 59401989 59402137 59401989 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
chr3.4 exon 59402851 59404209 59402851 ID=MS.gene04250.t1.exon8;Parent=MS.gene04250.t1
chr3.4 CDS 59402851 59404209 59402851 ID=cds.MS.gene04250.t1;Parent=MS.gene04250.t1
Gene Sequence

>MS.gene04250

ATGGAGAAGCAGCAACAAGATGATGAATTTATGATGTATACATTCAAAGTGCGGCATTGCGGTTATGTTCATGATTGGAAAAAATGTCCCTTTGCTCATCATGGGGAAAGCATAAAACGCCGTGATCCGAAGCAATATAATTACAGATGTGCCCATTGTAATGAATTTCAGAAATATAGATCATGCAGTAAAGGGGATGAATGTCAATATGCACATGGTGTTCTTGAGTCCGGGCTGCATCCTAGTCGATACAGAACAACGCTTTGCAATAATGGAACTACATGCACAAGAGAAGTTTGCTTCTTTGCTCACAAACCTGAGGAACTTCGCCCGTTGAATGCTAGTACCAGTTCTGCTTTGCCTTCACATACATTATATTCAAATTCACCAAGTGCTTCTGCAATGTATACTTTCACATTGCCTTCATCGATGCCGCCTTGGACACCGTCTGCAATATCATATCCTGCAGGAAGAACCATGTGGCCGACTCCGACTCAGAATCGTACTACAGTCCCTACACCTCAGATGCCGAGCAACAGATTGATATCATATCCTGCAGGAAGAACCATGTGGCCGACTCCGACTCAGAATCGTACTACAGTCCCTACACCTCAGATGCCGAGCAACAGATTGATATCATATCCTGCAGAAAGAACCATGTGGCCGACTCCGACTCAGATTAGTACTACAGTCCCTACACCTCAGATGCCGAGCAACAGATTGAATACTGCATTGAATGCTAGAGATAATACTCATCTGGACATGAAATTTTTCGAATATCACAATATGCCGAATCCAATGATAGAAGGGTCAAGTAGGCTTGCTGGAGTGAATCCTATTAACCTTGAAGGCTATTTTGGGTCATCGATACAATCTCCGACAGCAATGCAGGGGTATCAGAATGTGAACCAACAACTTCAGATATATCCTTCTCACCTTACCAATTCTAATGTGGTTAGATCACAACAGTTCAGGGGTGATCCATTTTTTAATTCAAGATATGATGCCCTTTCGAGGCAGAGCCAGAACTTTATCGAGAGCAGCAGCATGGCAAGCTCCAAGCTTCCAGATTCTACCTCAGTTGCATTGGAGCCTTCAACTGCCTTCTCCGGTTGGCGCTCCCGTGATAGAAAATTAGAATTGGGTGACACTAGCAACACTTCAACAATGGCTGCTACATCAAATGTTGATGATCAGGATGGATTATTAAGATATGAAGGTATGGTTAATGAACTTGTGAAGGAAGACGATCCAACATTGTAATGTTGAAGTTGAAGATAAACTTCAGTGCCATAATCCGGACGATGTTCCATCTTAGCCGAAGCAGTTATACATGTTATTCACCTGTGATTTTTTTTAACTTTAGAGATATTCATTATATTTGGGTGACACTGCAGAAAATATGGATAGATTGCTAGTATATTGTTCCATTTTTTTTCCCTTAATTCTTTGTTTTGTTATTTGTTATGTTCAATAGTGATACAAAGATTCATGAATATGCATATGCTTATAATGGTATGAAGTTAAAAATGAAAGCTCACCCTTCATTTGGTTAAAACATGTATCCAAACATATGCCTTGAATTATTGTTTCATAGATACTGTCATCACAATAGTTTTGTGCTTGCTTGCGCGATGAAATACAATTGTTGCGTGTATGTTTGAATATGCGGTGGGAGGTTCCAAAATTGCAGCGAAATATCACGTTTACGTTGAAGCCGCAAATAGTAGCTTCTGGTTTTCATGGTGAGAAGACCATCAGTCGTGCGACTGCAGCAACAAAACATGTATCCAAACATACGCCTTGAATTATGGTTTGTAACTAAGAAGCACAGATGTTCTTCATTTTCGCTTTTTATCAAGGTAAAAAATATGTATCAAACTACCACTCTTTTGGAAATAAACACACTACTACCATTCTCGTTTTTTGAATGTACGCTAGTTTACTGATTTTGGGACACGTGATTTTTTAAATTGAAATATTGTCATAAAGTAAACTTGTGTCCAATCTTATACCATTTTATTCATAAGGTTTAAATTTTTTACAAATTTTGAAGTTACCAACTTGTGAGTAGTTACTTGCAAGTTTACTTACCACTTCATTCAACAATTGATCAAAAATTTCTGACTCAAATTCAGCACATATGCTTCCTAAATCAGTTTGTCAGATGCTGAGGTTCTATTTTTGAAAAAAAGTAATATGTGTGCCTAAATCATTTTCTTTTCGCGTGTATAAAATAATATGTTTTATCTTTCACTCTGGAAGATGTACAGCTTGTTTTTGCCGTTTGGTTAATAAGCTTATGTAACAGTTTACCGCTTTGTTTTCTTTTTTCTTTTCTATTACGAAGGTTTATGTTTCTTTTTAGATTAATTGTTGTGCACAAACTAAGATCTTAAATAATACTGTGATAAACTGTATTATTATTGCAAATTTTATTTTATTTTTTGTTTAAACATTGTTGCAAATTTCTTGGGATTGATGATTTTATAACAAATTTAACAGACTTCCTTTCCATTTCCCTATCTGATTCCTTTCTGTTTAGTATCATATTAAAACACGTTTTCTCGTTTGAGTTTGTAAATTCTGTATAATAATAGAGAGGAAGACAAATATACTGATAAAATAAAAGTCATGTGCAACGATACGAATGGAAAACTTTGTCAAACTAGTTTGATTAGGGAAAATGAATAGGGAACACAAGAACTTCATCATAAGATTTCAGCTTTGCTTGAGTTTTCAGCCAAAGGTTATACTATGGTCGAAGCTCCTCGGGTTTGTGAGCAAAAAAGCAAACTCTTCTGGTGCAATAGGTTTCATACTTGCTAAGCCTTGTTCTATATTGACCAGGATGAAGCCAGAACTCGAAAATACCGTGTGCATATTCACAAGCATCCCCTTTACTGTATGATTCCTTACGAAACTCAGGACAAGGGACACAAATCAGCAACTTTGGGGCTATCCTTCTGATCTTTCCAATTCAAATGTGAGTGGATCACAACAATTCAGGGTTGATGCCTTTTCGAAACGGAGCCAGAGCTTTATTGAGAGCAATAGCGGGGTGAGCTTCAATTCTTTGCTTCCATCTGCGGTGATGAACTCAATAAAATGAGGAAATCTTATTCTTTCGGCTTTCAAAACAGAAGTTTCACTTCAACTGCTGCATTTAATATCGATGATCCAGATGTTGAAGATCCACGCCAATGCTATAATCCAGACGCGGTTTCGTCTTTGCCAGAGAAATTGTACATGGAGCATAAGCAGAAGGTGGTGTGAATCTAATGTTAACTTTGACATTGGATCCTTTGTGCATCTCTAGGCTAGTTTATTTATTAAATGTTTTTATTTTATTTTATTTTATTTTATTTTATAGATCTTAATTAAATATAGGTAATATTTTGCAGAAAATATGGTATAGTGTCGATAATACAAAAGTTAGGACAGAATGCAAGTATTTTGCATCAGTAACAACTTGTTACTACTACAAGGATATAGTATATGGTTCCTTTTTTTTCTCTCTATATCTTTGCTTTGTTAATCCTTCTTAAATCTGTATTGACATTTTGTTCACTCAAAAGCACAAAGTTAAGATCAGAATTTTCTTAATTCTAATCAAGAAAGTTTGTTATCCTCAAAATGCAAAATTAAGGATTTTGTCTCTACACGATTAATTCACCTGATACGAGATATATATATACAGTTACAGAATGAATTTTCATAATAAAAAAAGACACTTGACCGAATTTCTTTCTTCAATATAACAAGCGTGAGACATTAGAAATGCGTGACCAATATATACAAAATACACCTCATGTAGAGTAACTTTGATTGTAAAACAAGAAACCAACTCAACTAACTCTCTCTATGAAATCTGGAACTTGATATAACAGCTTCTAACTGTTTTTCTAACTGTCTTGAACTGATCTATTTAGCAGTGACTAGTGCAGCAGCTTGGTGGGCTTTTCTTTTTGACCTCAAAACCGTAAAACAACAAAAGACACTGAAAGAGTCTACCACTTCTTCCTTTGCATTGTTTTCCAAGTTTCTAAACCTTCACCAAGTTTCGGTGCAGTTTCCTTATTCTGTAAGTTTTTCTTTTATAATTCTAGATTTATGTATTTTTCTTATTCTTCACGATGAATCAGTGAATTTCACCAAAAGATGAGATCTTTTCTAAAATTATATGAGATTGCAATGTGTTTTCTTGGATGCTAAGAAAGTTCCTTAAAAAGGGTGTGTTCTGAATTATTTGGGTTTGTCTTAGTAAGAGTGTACTTTTCTTATTCTTGATGGTTCGTTGAGTTTCGGCTAAAGATTTTATCTTTTCTTAAAGTATTTGAGATTACAATGTGTTTTCTTGGTTGCTAAGAAAGTTCCTGAAAAAGTGTTTCTTGAGAGTCTTGATGAATGTGTATGAAAGAACATGCTTTCTCTTTCACTCTCTAGAAGACAGAAAGAAGGTTCGATGATAGACAACTTTTTATATGCTTTGTCATATCAATGTTGGATGATACACAAAATAACCATTGTTGTGTTCTTAATCTAACAATATTAAATGCAGTTGGTCGATCGATAAAATTATCAAGCTGAACGTGTAGTTGTCATGAGTGTAGAATCCCAGAATTGTTTCTATTGTCTTGCTATTTATGTACTAAGGATGTGCAATAGTTTTCCATTGTTTGTGATTATTTTTATAGTTATTGTTGTGCATAAACTATGATATTAAACATTTAAAAATTAAAATAATATTTGTTGCCAATTTCCTGGTGATTGATGATTTTGTAATAAATTTAACAGAACCAAGATTGCCTCAAGGGAGAAGGTTTCGAAATGGAGAAGCAGAAACAACATGTAGGTGGACCTCAAACTAAGAAGAAAGATTATCGGATCGATCGATCCCTACCTGACATAAAGGATAGGATATATATTACAGATGAATTTAGGATGTATAAATTCAAAGTGAAGCTTTGCTCATGGGATAATACTCCTCATGAGTGGACAGAATGTCCCTTTGCTCATTCTGGGGAAATTGCAAGGCGCCGTGATCCGAAGAAATATTATTACAGCTGTGTCTATTGTCATGAATTTCAGAATGGATCATGCAGTAGAGGGGATACATGTCAATATTCACATGGTGTTTTTGAGTCCGGGCTGCATCCTAGTCGATACAGAACAAAGCTTTGCAATAATGGAACTAAATGCACAAGAGAAATTTGCTTCTTTGCTCACAAACCCGAGGAGCTTCGCCTGTTGAAGGCTTATACCAGTTCTGCTTTTCCTTCACCTACATCAAATTCAAATTCACCTAGTGTTTCTTCAATGGATTCTTTCACATTGATCTCTCCGTCTTCTTTGATACAATCTTCGTCGACACTGCCTTGGACTCCGTCTGCAACATCATCTCCTGCAGGAGGAACCATGTGGCAGACTCCGACTCAGATTCATACTACAGTCCCCACACTTCAGATGCCAAGCAACAGATTGAAAACTGCATTGAATGCTAGAGATAATACTGATCTAGACATGAAGTTTCTTGAAAATCACCATATGCCGAAGCCAATGATAGAAGAGTCAAATAGGCTTGCAGGAGTGAATCCTACTAACCATGAAGACATTTTCGGGTCATCGATACAATCTTCGAAAGACATGCTGGTGCATCAGAATGTGAACCAAGAACTGTGTGACTACCCTTCTGACCTAACCAATTCAAATGTGATTGGATCACCACAACTCAGCGTTGATCCATTTATGAATTCAAGCTATGATGCCATTTCGAATCGGAGCCAGAGCTTTATCGAGCACAGCAGCGTGGCAAGCTTTAGTTACAAGTTTCCTTCTGCTACCTCAGTTGCATTAGAGCCTTCTACTGCCTTCTCTGATTGGTACTCCACTGATAGAAAGGTAGATTGGTCTATCCCCGGTGATAAACTCAATGAAATGACGACGTCTTATTCATTCGGCTTCCTAGACGATACTAGCAATGTTTCAACAATGGCTGCTGCATCAAATGTTGATGAGAATTATTCATTATTAAGCCAGGAATCTTGGGTTAGCTCACTTGTGAAGGATGATGATCCAACATTGGAATCTGATCAACATTGTGTTGAAGTTGAATATCAACTACAATGCCATAATCCGGACGATGTTCCGTCCTAG
Protein sequence

>MS.gene04250.t1

MEKQQQDDEFMMYTFKVRHCGYVHDWKKCPFAHHGESIKRRDPKQYNYRCAHCNEFQKYRSCSKGDECQYAHGVLESGLHPSRYRTTLCNNGTTCTREVCFFAHKPEELRPLNASTSSALPSHTLYSNSPSASAMYTFTLPSSMPPWTPSAISYPAGRTMWPTPTQNRTTVPTPQMPSNRLISYPAGRTMWPTPTQNRTTVPTPQMPSNRLISYPAERTMWPTPTQISTTVPTPQMPSNRLNTALNARDNTHLDMKFFEYHNMPNPMIEGSSRLAGVNPINLEGYFGSSIQSPTAMQGYQNVNQQLQIYPSHLTNSNVVRSQQFRGDPFFNSRYDALSRQSQNFIESSSMASSKLPDSTSVALEPSTAFSGWRSRDRKLELGDTSNTSTMAATSNVDDQDGLLRYEALLEFSAKGYTMVEAPRDEARTRKYRVHIHKHPLYWLMPFRNGARALLRAIAGSFTSTAAFNIDDPDVEDPRQCYNPDAVSSLPEKLYMEHKQKKIWYSVDNTKVRTECKYFASVTTCYYYKDIVYVTSAAAWWAFLFDLKTVKQQKTLKESTTSSFALFSKFLNLHQVSVQFPYSNQDCLKGEGFEMEKQKQHVGGPQTKKKDYRIDRSLPDIKDRIYITDEFRMYKFKVKLCSWDNTPHEWTECPFAHSGEIARRRDPKKYYYSCVYCHEFQNGSCSRGDTCQYSHGVFESGLHPSRYRTKLCNNGTKCTREICFFAHKPEELRLLKAYTSSAFPSPTSNSNSPSVSSMDSFTLISPSSLIQSSSTLPWTPSATSSPAGGTMWQTPTQIHTTVPTLQMPSNRLKTALNARDNTDLDMKFLENHHMPKPMIEESNRLAGVNPTNHEDIFGSSIQSSKDMLVHQNVNQELCDYPSDLTNSNVIGSPQLSVDPFMNSSYDAISNRSQSFIEHSSVASFSYKFPSATSVALEPSTAFSDWYSTDRKVDWSIPGDKLNEMTTSYSFGFLDDTSNVSTMAAASNVDENYSLLSQESWVSSLVKDDDPTLESDQHCVEVEYQLQCHNPDDVPS