AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042618

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042618.t1 MTR_5g055260 76.103 272 37 4 1 272 1 244 2.50e-138 392
MS.gene042618.t1 MTR_4g021790 73.384 263 69 1 1 263 1 262 9.99e-136 386
MS.gene042618.t1 MTR_4g021855 63.245 302 101 2 1 293 1 301 3.52e-129 370
MS.gene042618.t1 MTR_4g021760 61.258 302 114 3 1 300 1 301 1.13e-125 362
MS.gene042618.t1 MTR_4g021845 70.642 218 63 1 1 218 1 217 1.21e-107 312
MS.gene042618.t1 MTR_1g032570 46.371 248 101 4 10 226 11 257 2.88e-67 221
MS.gene042618.t1 MTR_4g023980 64.493 138 48 1 55 192 35 171 6.96e-61 192
MS.gene042618.t1 MTR_2g034960 42.205 263 124 4 10 248 29 287 1.13e-60 204
MS.gene042618.t1 MTR_4g121020 44.262 244 107 4 10 226 31 272 1.94e-58 199
MS.gene042618.t1 MTR_4g121020 44.262 244 107 4 10 226 31 272 2.04e-58 199
MS.gene042618.t1 MTR_3g086100 40.816 245 116 5 10 226 13 256 5.23e-54 185
MS.gene042618.t1 MTR_6g045327 44.939 247 119 7 1 237 1 240 3.86e-51 173
MS.gene042618.t1 MTR_3g106220 39.759 249 117 4 10 226 8 255 7.30e-51 178
MS.gene042618.t1 MTR_8g079940 38.525 244 123 4 9 226 24 266 1.60e-50 175
MS.gene042618.t1 MTR_4g131600 40.254 236 117 5 9 221 19 253 5.37e-49 172
MS.gene042618.t1 MTR_8g105600 39.357 249 131 2 1 230 1 248 1.54e-48 171
MS.gene042618.t1 MTR_1g013170 38.554 249 122 5 7 226 2 248 3.18e-47 166
MS.gene042618.t1 MTR_2g450070 34.510 255 129 4 10 227 18 271 3.36e-46 166
MS.gene042618.t1 MTR_2g450070 34.510 255 129 4 10 227 18 271 4.61e-46 165
MS.gene042618.t1 MTR_4g131580 37.500 248 129 4 10 232 17 263 4.47e-45 162
MS.gene042618.t1 MTR_4g131570 38.776 245 121 6 10 226 17 260 9.26e-45 160
MS.gene042618.t1 MTR_7g117705 35.484 248 129 5 7 225 2 247 1.22e-43 155
MS.gene042618.t1 MTR_1g013180 37.647 255 128 6 1 226 147 399 2.04e-43 158
MS.gene042618.t1 MTR_8g093040 43.049 223 107 6 10 230 14 218 2.58e-43 150
MS.gene042618.t1 MTR_1g032570 42.328 189 78 3 68 226 1 188 2.12e-41 151
MS.gene042618.t1 MTR_3g102590 35.909 220 113 4 10 226 8 202 4.58e-41 143
MS.gene042618.t1 MTR_3g102600 36.239 218 102 3 10 226 8 189 4.74e-40 140
MS.gene042618.t1 MTR_3g102590 35.238 210 110 3 10 218 8 192 2.72e-38 134
MS.gene042618.t1 MTR_6g016850 42.593 162 91 2 1 161 1 161 1.51e-37 132
MS.gene042618.t1 MTR_3g102600 34.434 212 102 3 10 220 8 183 4.42e-36 129
MS.gene042618.t1 MTR_3g086100 37.745 204 97 5 68 242 1 203 9.17e-34 129
MS.gene042618.t1 MTR_3g102600 35.429 175 77 2 52 226 2 140 3.64e-31 115
MS.gene042618.t1 MTR_4g121020 42.424 165 67 3 88 226 6 168 9.59e-29 116
MS.gene042618.t1 MTR_7g026400 37.143 210 99 9 45 231 19 218 7.00e-28 114
MS.gene042618.t1 MTR_3g102600 33.136 169 77 2 52 220 2 134 4.59e-27 103
MS.gene042618.t1 MTR_0450s0040 31.441 229 140 5 14 231 8 230 6.14e-27 108
MS.gene042618.t1 MTR_5g041350 51.948 77 34 2 165 240 11 85 6.72e-16 77.8
MS.gene042618.t1 MTR_7g098250 48.649 74 37 1 165 237 258 331 1.32e-15 77.4
MS.gene042618.t1 MTR_2g016220 34.884 129 77 3 154 276 69 196 2.39e-15 76.3
MS.gene042618.t1 MTR_4g064730 49.315 73 36 1 155 226 116 188 2.77e-15 75.5
MS.gene042618.t1 MTR_2g016220 34.884 129 77 3 154 276 69 196 3.07e-15 75.9
MS.gene042618.t1 MTR_1g013160 29.333 150 76 4 71 192 1 148 4.72e-15 72.0
MS.gene042618.t1 MTR_7g089010 57.627 59 25 0 168 226 90 148 5.81e-15 74.3
MS.gene042618.t1 MTR_6g032990 54.839 62 25 2 169 229 42 101 9.12e-15 74.7
MS.gene042618.t1 MTR_7g115530 48.571 70 35 1 169 237 218 287 1.33e-14 73.2
MS.gene042618.t1 MTR_7g115530 48.571 70 35 1 169 237 218 287 2.18e-14 73.2
MS.gene042618.t1 MTR_2g016220 34.586 133 76 5 154 276 69 200 2.44e-14 73.2
MS.gene042618.t1 MTR_2g086450 59.259 54 21 1 169 221 45 98 3.06e-14 73.2
MS.gene042618.t1 MTR_7g115530 48.571 70 35 1 169 237 218 287 3.12e-14 73.2
MS.gene042618.t1 MTR_1g080330 43.678 87 46 2 165 250 171 255 7.65e-14 70.9
MS.gene042618.t1 MTR_7g115530 59.259 54 21 1 169 221 218 271 9.31e-14 71.2
MS.gene042618.t1 MTR_0223s0040 44.706 85 44 2 167 250 16 98 1.01e-13 70.5
MS.gene042618.t1 MTR_3g450310 47.826 69 35 1 161 228 11 79 1.24e-13 69.3
MS.gene042618.t1 MTR_1g080330 57.407 54 22 1 169 221 175 228 1.42e-13 70.9
MS.gene042618.t1 MTR_7g088070 49.333 75 35 2 160 231 41 115 3.35e-13 68.9
MS.gene042618.t1 MTR_7g069660 51.786 56 26 1 167 221 69 124 3.79e-13 69.7
MS.gene042618.t1 MTR_4g081710 56.897 58 24 1 165 221 13 70 3.83e-13 69.3
MS.gene042618.t1 MTR_8g086410 45.588 68 36 1 155 221 95 162 4.66e-13 69.7
MS.gene042618.t1 MTR_8g086410 45.588 68 36 1 155 221 97 164 4.73e-13 69.7
MS.gene042618.t1 MTR_7g088070 44.828 87 45 2 160 243 41 127 5.32e-13 68.6
MS.gene042618.t1 MTR_1g112370 47.143 70 36 1 154 222 7 76 1.08e-12 68.2
MS.gene042618.t1 MTR_8g086410 40.741 81 43 2 155 230 97 177 1.26e-12 68.6
MS.gene042618.t1 MTR_8g024260 29.268 123 82 3 14 132 36 157 1.28e-12 68.6
MS.gene042618.t1 MTR_1g067110 29.839 124 80 4 14 132 85 206 1.59e-12 68.6
MS.gene042618.t1 MTR_1g067110 29.839 124 80 4 14 132 85 206 1.65e-12 68.6
MS.gene042618.t1 MTR_2g023580 50.877 57 27 1 167 222 53 109 1.83e-12 66.2
MS.gene042618.t1 MTR_2g027800 43.750 80 43 2 143 221 14 92 2.16e-12 67.0
MS.gene042618.t1 MTR_2g027800 43.750 80 43 2 143 221 14 92 2.51e-12 67.0
MS.gene042618.t1 MTR_7g115530 57.692 52 21 1 169 219 218 269 2.54e-12 66.2
MS.gene042618.t1 MTR_1g090670 57.407 54 22 1 169 221 44 97 3.05e-12 66.2
MS.gene042618.t1 MTR_2g090675 38.750 80 49 0 146 225 138 217 3.17e-12 67.0
MS.gene042618.t1 MTR_5g014040 31.496 127 81 4 10 134 14 136 3.88e-12 67.0
MS.gene042618.t1 MTR_5g014040 43.662 71 40 0 158 228 300 370 2.49e-11 64.7
MS.gene042618.t1 MTR_5g014040 31.496 127 81 4 10 134 14 136 4.05e-12 67.0
MS.gene042618.t1 MTR_6g444980 57.407 54 22 1 169 221 35 88 6.90e-12 65.9
MS.gene042618.t1 MTR_6g444980 57.407 54 22 1 169 221 35 88 8.08e-12 65.5
MS.gene042618.t1 MTR_4g098870 46.575 73 38 1 164 236 325 396 8.35e-12 66.2
MS.gene042618.t1 MTR_4g098870 46.575 73 38 1 164 236 305 376 8.50e-12 66.2
MS.gene042618.t1 MTR_7g093030 38.710 93 47 3 167 258 210 293 1.19e-11 64.7
MS.gene042618.t1 MTR_4g098870 46.575 73 38 1 164 236 325 396 1.22e-11 65.5
MS.gene042618.t1 MTR_4g098870 46.575 73 38 1 164 236 305 376 1.27e-11 65.5
MS.gene042618.t1 MTR_4g098870 46.575 73 38 1 164 236 344 415 1.29e-11 65.5
MS.gene042618.t1 MTR_7g093030 50.000 56 27 1 167 221 210 265 1.47e-11 65.1
MS.gene042618.t1 MTR_7g115530 56.863 51 21 1 169 218 218 268 1.62e-11 64.3
MS.gene042618.t1 MTR_4g078810 48.276 58 30 0 168 225 157 214 1.66e-11 65.1
MS.gene042618.t1 MTR_2g027860 52.055 73 31 3 169 237 49 121 1.94e-11 64.3
MS.gene042618.t1 MTR_2g084230 47.368 57 29 1 167 222 95 151 2.12e-11 63.9
MS.gene042618.t1 MTR_2g084230 47.368 57 29 1 167 222 95 151 2.24e-11 64.3
MS.gene042618.t1 MTR_5g017980 50.820 61 30 0 166 226 189 249 3.34e-11 63.5
MS.gene042618.t1 MTR_5g017980 50.820 61 30 0 166 226 190 250 3.39e-11 63.5
MS.gene042618.t1 MTR_1g053800 54.386 57 25 1 169 224 177 233 3.49e-11 63.5
MS.gene042618.t1 MTR_3g092780 29.365 126 84 3 14 135 56 180 3.86e-11 64.3
MS.gene042618.t1 MTR_5g027440 51.852 54 25 1 169 221 25 78 4.28e-11 62.8
MS.gene042618.t1 MTR_3g092780 29.268 123 82 3 14 132 56 177 4.47e-11 63.9
MS.gene042618.t1 MTR_3g092780 29.365 126 84 3 14 135 56 180 4.75e-11 63.9
MS.gene042618.t1 MTR_1g053835 40.541 74 41 2 167 239 256 327 4.88e-11 63.5
MS.gene042618.t1 MTR_3g092780 29.268 123 82 3 14 132 56 177 5.65e-11 63.5
MS.gene042618.t1 MTR_7g118260 28.030 132 88 4 11 137 11 140 7.27e-11 63.2
MS.gene042618.t1 MTR_4g086835 47.619 63 33 0 164 226 269 331 8.46e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042618.t1 AT2G01760 41.569 255 113 5 7 226 4 257 4.30e-57 189
MS.gene042618.t1 AT3G16857 40.684 263 109 3 10 226 33 294 1.35e-56 194
MS.gene042618.t1 AT3G16857 40.684 263 109 3 10 226 33 294 1.52e-56 194
MS.gene042618.t1 AT2G01760 37.456 283 113 6 7 226 4 285 3.84e-51 174
MS.gene042618.t1 AT1G67710 40.408 245 116 5 11 226 8 251 4.23e-49 171
MS.gene042618.t1 AT2G25180 39.834 241 120 5 10 226 13 252 2.87e-47 167
MS.gene042618.t1 AT4G31920 37.097 248 141 5 10 244 13 258 2.15e-44 159
MS.gene042618.t1 AT3G16857 39.286 224 89 3 49 226 2 224 3.24e-42 154
MS.gene042618.t1 AT5G58080 36.232 276 144 7 10 259 14 283 1.84e-41 152
MS.gene042618.t1 AT2G01760 39.896 193 81 5 68 226 1 192 3.22e-34 127
MS.gene042618.t1 AT3G62670 34.821 224 120 7 28 227 53 274 4.76e-33 126
MS.gene042618.t1 AT1G49190 31.429 245 132 6 10 221 30 271 2.18e-32 124
MS.gene042618.t1 AT5G49240 30.802 237 136 5 9 218 37 272 3.28e-29 113
MS.gene042618.t1 AT1G67710 38.503 187 86 4 68 226 1 186 7.10e-29 115
MS.gene042618.t1 AT5G07210 26.937 271 141 6 14 230 16 283 5.75e-22 96.7
MS.gene042618.t1 AT1G67710 37.725 167 75 4 88 226 12 177 2.15e-20 91.7
MS.gene042618.t1 AT1G49190 37.302 126 77 2 10 134 30 154 1.44e-19 89.7
MS.gene042618.t1 AT5G07210 26.165 279 141 7 14 230 16 291 1.74e-19 89.4
MS.gene042618.t1 AT1G68210 25.185 270 144 7 8 221 7 274 8.79e-17 81.3
MS.gene042618.t1 AT5G59570 55.000 60 27 0 167 226 139 198 2.08e-15 75.9
MS.gene042618.t1 AT5G59570 55.000 60 27 0 167 226 139 198 2.08e-15 75.9
MS.gene042618.t1 AT5G05090 57.627 59 25 0 168 226 80 138 2.40e-15 75.1
MS.gene042618.t1 AT2G27070 25.418 299 159 9 1 242 3 294 3.12e-15 76.6
MS.gene042618.t1 AT3G10760 57.627 59 25 0 168 226 104 162 4.07e-15 75.1
MS.gene042618.t1 AT2G40970 55.932 59 26 0 168 226 104 162 4.40e-15 73.9
MS.gene042618.t1 AT3G46640 55.932 59 26 0 168 226 143 201 6.74e-15 74.7
MS.gene042618.t1 AT3G46640 55.932 59 26 0 168 226 143 201 6.94e-15 74.3
MS.gene042618.t1 AT3G46640 55.932 59 26 0 168 226 143 201 6.94e-15 74.3
MS.gene042618.t1 AT3G04030 58.621 58 23 1 165 221 41 98 7.41e-15 75.1
MS.gene042618.t1 AT3G04030 58.621 58 23 1 165 221 41 98 7.90e-15 74.7
MS.gene042618.t1 AT5G18240 58.621 58 23 1 165 221 41 98 1.71e-14 73.9
MS.gene042618.t1 AT5G18240 58.621 58 23 1 165 221 41 98 1.71e-14 73.9
MS.gene042618.t1 AT5G18240 58.621 58 23 1 165 221 41 98 1.79e-14 73.9
MS.gene042618.t1 AT5G18240 58.621 58 23 1 165 221 41 98 1.79e-14 73.9
MS.gene042618.t1 AT5G18240 58.621 58 23 1 165 221 41 98 1.81e-14 73.9
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 2.94e-14 73.6
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 2.97e-14 73.6
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 2.97e-14 73.6
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 2.97e-14 73.6
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 3.86e-14 72.8
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 3.86e-14 72.8
MS.gene042618.t1 AT5G60100 32.258 124 77 4 14 132 64 185 3.86e-14 72.8
MS.gene042618.t1 AT4G13640 38.136 118 61 4 169 282 37 146 4.11e-14 72.0
MS.gene042618.t1 AT4G13640 38.136 118 61 4 169 282 37 146 4.88e-14 71.6
MS.gene042618.t1 AT2G01060 49.412 85 39 3 158 238 4 88 5.51e-14 71.6
MS.gene042618.t1 AT3G04030 54.386 57 26 0 165 221 41 97 7.25e-14 72.0
MS.gene042618.t1 AT4G04605 42.857 77 42 2 167 241 12 88 7.34e-14 70.1
MS.gene042618.t1 AT5G24470 30.709 127 83 3 13 135 49 174 1.76e-13 71.2
MS.gene042618.t1 AT1G69580 35.714 126 70 5 165 284 30 150 1.97e-13 70.5
MS.gene042618.t1 AT5G44190 53.448 58 27 0 168 225 146 203 2.39e-13 70.5
MS.gene042618.t1 AT5G44190 53.448 58 27 0 168 225 146 203 2.86e-13 70.1
MS.gene042618.t1 AT3G24120 36.975 119 61 5 169 282 41 150 4.33e-13 68.9
MS.gene042618.t1 AT3G24120 36.975 119 61 5 169 282 41 150 4.66e-13 68.9
MS.gene042618.t1 AT2G40260 45.205 73 39 1 151 222 64 136 5.83e-13 69.3
MS.gene042618.t1 AT2G20570 44.928 69 38 0 157 225 141 209 6.05e-13 69.3
MS.gene042618.t1 AT5G06800 44.304 79 37 3 169 240 192 270 7.43e-13 68.9
MS.gene042618.t1 AT1G69580 35.433 127 70 5 165 284 30 151 7.86e-13 68.6
MS.gene042618.t1 AT1G14600 55.556 54 23 1 170 222 24 77 1.50e-12 67.0
MS.gene042618.t1 AT5G06800 42.857 84 36 3 169 240 192 275 2.00e-12 67.8
MS.gene042618.t1 AT2G20570 54.545 55 25 0 171 225 171 225 2.76e-12 67.4
MS.gene042618.t1 AT2G42660 44.615 65 35 1 159 222 40 104 3.45e-12 65.9
MS.gene042618.t1 AT2G02060 33.962 106 61 3 170 269 30 132 3.87e-12 65.9
MS.gene042618.t1 AT2G02060 33.962 106 61 3 170 269 30 132 3.87e-12 65.9
MS.gene042618.t1 AT5G29000 32.000 125 71 4 126 236 175 299 3.92e-12 66.2
MS.gene042618.t1 AT4G28610 44.595 74 39 2 169 241 225 297 4.33e-12 66.6
MS.gene042618.t1 AT2G38300 49.123 57 28 1 167 222 52 108 6.11e-12 65.9
MS.gene042618.t1 AT3G04450 53.448 58 26 1 165 221 234 291 7.18e-12 66.2
MS.gene042618.t1 AT3G04450 53.448 58 26 1 165 221 234 291 7.68e-12 65.5
MS.gene042618.t1 AT3G04450 53.448 58 26 1 165 221 194 251 7.93e-12 65.9
MS.gene042618.t1 AT3G04450 53.448 58 26 1 165 221 194 251 7.93e-12 65.9
MS.gene042618.t1 AT5G06800 53.704 54 24 1 169 221 192 245 8.12e-12 65.1
MS.gene042618.t1 AT1G79430 57.407 54 22 1 169 221 34 87 8.37e-12 65.5
MS.gene042618.t1 AT3G12730 53.704 54 24 1 169 221 23 76 9.46e-12 64.3
MS.gene042618.t1 AT2G20400 55.556 54 23 1 169 221 231 284 9.69e-12 65.5
MS.gene042618.t1 AT2G20400 55.556 54 23 1 169 221 231 284 9.84e-12 65.5
MS.gene042618.t1 AT5G29000 31.250 128 74 4 126 239 175 302 1.10e-11 65.5
MS.gene042618.t1 AT5G29000 31.250 128 74 4 126 239 132 259 1.22e-11 65.1
MS.gene042618.t1 AT5G29000 31.250 128 74 4 126 239 132 259 1.22e-11 65.1
MS.gene042618.t1 AT3G13040 48.571 70 33 2 168 236 240 307 1.51e-11 65.1
MS.gene042618.t1 AT3G13040 48.571 70 33 2 168 236 240 307 1.51e-11 65.1
MS.gene042618.t1 AT3G13040 48.571 70 33 2 168 236 240 307 1.51e-11 65.1
MS.gene042618.t1 AT2G46790 29.032 124 81 4 14 132 37 158 1.66e-11 65.1
MS.gene042618.t1 AT2G46790 29.032 124 81 4 14 132 37 158 1.83e-11 65.1
MS.gene042618.t1 AT5G45580 53.704 54 24 1 169 221 7 60 2.20e-11 63.5
MS.gene042618.t1 AT5G45580 53.704 54 24 1 169 221 23 76 2.40e-11 63.5

Find 59 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAACTTTGGATGGAAAT+TGG 0.197434 5.2:-46859381 MS.gene042618:CDS
CATATGGGGCTTGCGAATAT+TGG 0.282422 5.2:-46860098 MS.gene042618:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 5.2:-46859693 MS.gene042618:intron
AATTCATCAGTTGATTATTT+CGG 0.292540 5.2:-46859291 MS.gene042618:CDS
GCAATAACGTTGAGACCATT+AGG 0.294493 5.2:+46860588 None:intergenic
GAATTAATCACGACACAATC+TGG 0.322128 5.2:+46859309 None:intergenic
TTCTGATGCCTTGAACTTGT+TGG 0.365455 5.2:-46860380 MS.gene042618:CDS
GTTACAAAGTCTATCACATA+TGG 0.379871 5.2:-46860114 MS.gene042618:CDS
TCTTGAAAATATTGGAAGCT+TGG 0.394226 5.2:-46859998 MS.gene042618:CDS
CTTGAACTTGTTGGAAAGAA+AGG 0.398808 5.2:-46860371 MS.gene042618:CDS
ATCAGAAGCTGTATTGCATT+TGG 0.403201 5.2:+46860396 None:intergenic
CAAGTAACTAACCTTGATAA+TGG 0.405402 5.2:+46860511 None:intergenic
CTTTCTTTCCAACAAGTTCA+AGG 0.409488 5.2:+46860372 None:intergenic
TTCCGGTGACCATGACAAAC+GGG 0.410735 5.2:+46859906 None:intergenic
CAATAACGTTGAGACCATTA+GGG 0.421209 5.2:+46860589 None:intergenic
GAACATCATCATGTTGGTTC+CGG 0.421484 5.2:+46859457 None:intergenic
CCCGTGGATAACTTTCATCT+TGG 0.433504 5.2:+46859418 None:intergenic
AAGATGATGCACAACAACTT+TGG 0.451734 5.2:-46859392 MS.gene042618:CDS
TTGAGACCATTAGGGAATGA+TGG 0.453801 5.2:+46860597 None:intergenic
AAGTCATAGGCATCCATGTT+TGG 0.461471 5.2:+46860313 None:intergenic
ATTGGAAGCTTGGATGTTCA+AGG 0.468841 5.2:-46859988 MS.gene042618:CDS
ATGTTCAAGGTCATAGAAAA+CGG 0.468876 5.2:-46859975 MS.gene042618:CDS
TGGCAGCATGTTGTAAGAAA+AGG 0.472283 5.2:-46860039 MS.gene042618:CDS
TTACAAAGTCTATCACATAT+GGG 0.475108 5.2:-46860113 MS.gene042618:CDS
TTCAAGCTCATCAGATTGAC+CGG 0.488681 5.2:-46859476 MS.gene042618:CDS
GTTCCGGTGACCATGACAAA+CGG 0.489781 5.2:+46859905 None:intergenic
TTGGACCAAGCCGCTGTATG+AGG 0.493917 5.2:-46860079 MS.gene042618:CDS
GACAACCCATCATTCCCTAA+TGG 0.494012 5.2:-46860603 MS.gene042618:CDS
TGTTCAAGGTCATAGAAAAC+GGG 0.501689 5.2:-46859974 MS.gene042618:CDS
GCCCCGTTTGTCATGGTCAC+CGG 0.505882 5.2:-46859908 MS.gene042618:CDS
ACATCTCCTCAATGGTGTAA+AGG 0.507135 5.2:+46860544 None:intergenic
ACACAATCTGGGAACAAGTT+GGG 0.508030 5.2:+46859321 None:intergenic
CACTTTCCTTTACACCATTG+AGG 0.511509 5.2:-46860550 MS.gene042618:CDS
AGGGAATGATGGGTTGTCAA+TGG 0.519036 5.2:+46860608 None:intergenic
TGATGGGTTGTCAATGGAAA+GGG 0.530511 5.2:+46860614 None:intergenic
TGATGCACAACAACTTTGGA+TGG 0.538898 5.2:-46859388 MS.gene042618:CDS
AGCATGTTGAACAAAGTCAT+AGG 0.540588 5.2:+46860300 None:intergenic
TGTAATCGATGCCATTATCA+AGG 0.541272 5.2:-46860522 MS.gene042618:intron
CTCAATGGTGTAAAGGAAAG+TGG 0.543869 5.2:+46860551 None:intergenic
TTGAATTGTTCCTCATACAG+CGG 0.552576 5.2:+46860069 None:intergenic
GACACAATCTGGGAACAAGT+TGG 0.557153 5.2:+46859320 None:intergenic
TGAGACCATTAGGGAATGAT+GGG 0.561537 5.2:+46860598 None:intergenic
TTATGGGCTATCAATCAACT+AGG 0.563246 5.2:-46859868 MS.gene042618:CDS
CATCTCGAACATCATCATGT+TGG 0.563402 5.2:+46859451 None:intergenic
TCGATTACACATCTCCTCAA+TGG 0.564409 5.2:+46860536 None:intergenic
AATTAATCACGACACAATCT+GGG 0.566059 5.2:+46859310 None:intergenic
CGAAGAAGCCCCGTTTGTCA+TGG 0.567178 5.2:-46859915 MS.gene042618:CDS
TTTGTAACAGAACTTAATGT+GGG 0.567790 5.2:+46860129 None:intergenic
GATGTTCATGGTTTGAGTGT+AGG 0.572619 5.2:-46859716 MS.gene042618:CDS
TTGTTCCTCATACAGCGGCT+TGG 0.580501 5.2:+46860074 None:intergenic
CTTTGTAACAGAACTTAATG+TGG 0.582713 5.2:+46860128 None:intergenic
ACCAAGATGAAAGTTATCCA+CGG 0.600866 5.2:-46859419 MS.gene042618:CDS
ATGATGGGTTGTCAATGGAA+AGG 0.601102 5.2:+46860613 None:intergenic
CCAAGATGAAAGTTATCCAC+GGG 0.623977 5.2:-46859418 MS.gene042618:CDS
AGGAACAATTCAAGAACATG+TGG 0.632433 5.2:-46860059 MS.gene042618:CDS
TGATGTTCATATGCCAAACA+TGG 0.637365 5.2:-46860326 MS.gene042618:CDS
TACAAAGTCTATCACATATG+GGG 0.656295 5.2:-46860112 MS.gene042618:CDS
TGTGCATCATCTTGTTCCCG+TGG 0.659103 5.2:+46859402 None:intergenic
TCCGGTGACCATGACAAACG+GGG 0.701794 5.2:+46859907 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATCTTGAAAATAT+TGG - chr5.2:46859899-46859918 MS.gene042618:CDS 10.0%
!!! TGATTTTAAATTTAAATTTG+TGG - chr5.2:46860290-46860309 MS.gene042618:CDS 10.0%
!! TAAAATGATTATCAAAAAGA+TGG - chr5.2:46860167-46860186 MS.gene042618:intron 15.0%
!!! ATCTTTTTGATAATCATTTT+AGG + chr5.2:46860168-46860187 None:intergenic 15.0%
!!! TATGTAAAATCAAGAATTTT+TGG + chr5.2:46859464-46859483 None:intergenic 15.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5.2:46859649-46859668 None:intergenic 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5.2:46860614-46860633 MS.gene042618:CDS 20.0%
!! TGTTTGTATTATTTAACAAC+TGG - chr5.2:46860100-46860119 MS.gene042618:CDS 20.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5.2:46860555-46860574 None:intergenic 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr5.2:46860083-46860102 None:intergenic 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5.2:46860279-46860298 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG - chr5.2:46859792-46859811 MS.gene042618:intron 25.0%
! TTTGTAACAGAACTTAATGT+GGG + chr5.2:46859779-46859798 None:intergenic 25.0%
AATTAATCACGACACAATCT+GGG + chr5.2:46860598-46860617 None:intergenic 30.0%
ATCAAAAAGATGGATGTTCA+TGG - chr5.2:46860177-46860196 MS.gene042618:intron 30.0%
ATGTTCAAGGTCATAGAAAA+CGG - chr5.2:46859930-46859949 MS.gene042618:CDS 30.0%
CAAGTAACTAACCTTGATAA+TGG + chr5.2:46859397-46859416 None:intergenic 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5.2:46860378-46860397 MS.gene042618:CDS 30.0%
CTTTGTAACAGAACTTAATG+TGG + chr5.2:46859780-46859799 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG - chr5.2:46859791-46859810 MS.gene042618:intron 30.0%
TACAAAGTCTATCACATATG+GGG - chr5.2:46859793-46859812 MS.gene042618:intron 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5.2:46860377-46860396 MS.gene042618:CDS 30.0%
TCTTGAAAATATTGGAAGCT+TGG - chr5.2:46859907-46859926 MS.gene042618:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5.2:46860020-46860039 MS.gene042618:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5.2:46860021-46860040 MS.gene042618:CDS 30.0%
! CATTATCAAGGTTAGTTACT+TGG - chr5.2:46859395-46859414 MS.gene042618:CDS 30.0%
!! AACTCTAGAATTGATGAGTA+AGG + chr5.2:46859430-46859449 None:intergenic 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5.2:46860137-46860156 MS.gene042618:CDS 30.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5.2:46860019-46860038 None:intergenic 35.0%
AAGATGATGCACAACAACTT+TGG - chr5.2:46860513-46860532 MS.gene042618:intron 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr5.2:46860212-46860231 MS.gene042618:intron 35.0%
ACCAAGATGAAAGTTATCCA+CGG - chr5.2:46860486-46860505 MS.gene042618:intron 35.0%
AGCATGTTGAACAAAGTCAT+AGG + chr5.2:46859608-46859627 None:intergenic 35.0%
AGGAACAATTCAAGAACATG+TGG - chr5.2:46859846-46859865 MS.gene042618:intron 35.0%
ATCAGAAGCTGTATTGCATT+TGG + chr5.2:46859512-46859531 None:intergenic 35.0%
CAACAACTTTGGATGGAAAT+TGG - chr5.2:46860524-46860543 MS.gene042618:CDS 35.0%
CAATAACGTTGAGACCATTA+GGG + chr5.2:46859319-46859338 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG + chr5.2:46859536-46859555 None:intergenic 35.0%
GAATTAATCACGACACAATC+TGG + chr5.2:46860599-46860618 None:intergenic 35.0%
TGATGTTCATATGCCAAACA+TGG - chr5.2:46859579-46859598 MS.gene042618:intron 35.0%
TGTAATCGATGCCATTATCA+AGG - chr5.2:46859383-46859402 MS.gene042618:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG - chr5.2:46859931-46859950 MS.gene042618:CDS 35.0%
TTGAATTGTTCCTCATACAG+CGG + chr5.2:46859839-46859858 None:intergenic 35.0%
! TCAACTAGGACTTGATAGTA+TGG - chr5.2:46860051-46860070 MS.gene042618:CDS 35.0%
! TTATGGGCTATCAATCAACT+AGG - chr5.2:46860037-46860056 MS.gene042618:CDS 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG - chr5.2:46859534-46859553 MS.gene042618:CDS 35.0%
AAGTCATAGGCATCCATGTT+TGG + chr5.2:46859595-46859614 None:intergenic 40.0%
ACACAATCTGGGAACAAGTT+GGG + chr5.2:46860587-46860606 None:intergenic 40.0%
ACATCTCCTCAATGGTGTAA+AGG + chr5.2:46859364-46859383 None:intergenic 40.0%
ATTGGAAGCTTGGATGTTCA+AGG - chr5.2:46859917-46859936 MS.gene042618:CDS 40.0%
CACTTTCCTTTACACCATTG+AGG - chr5.2:46859355-46859374 MS.gene042618:CDS 40.0%
CATCTCGAACATCATCATGT+TGG + chr5.2:46860457-46860476 None:intergenic 40.0%
CCAAGATGAAAGTTATCCAC+GGG - chr5.2:46860487-46860506 MS.gene042618:intron 40.0%
CTCAATGGTGTAAAGGAAAG+TGG + chr5.2:46859357-46859376 None:intergenic 40.0%
GATGTTCATGGTTTGAGTGT+AGG - chr5.2:46860189-46860208 MS.gene042618:intron 40.0%
GCAATAACGTTGAGACCATT+AGG + chr5.2:46859320-46859339 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG + chr5.2:46859372-46859391 None:intergenic 40.0%
TGAGACCATTAGGGAATGAT+GGG + chr5.2:46859310-46859329 None:intergenic 40.0%
TGATGCACAACAACTTTGGA+TGG - chr5.2:46860517-46860536 MS.gene042618:intron 40.0%
TGGCAGCATGTTGTAAGAAA+AGG - chr5.2:46859866-46859885 MS.gene042618:CDS 40.0%
TTCAAGCTCATCAGATTGAC+CGG - chr5.2:46860429-46860448 MS.gene042618:intron 40.0%
TTCTGATGCCTTGAACTTGT+TGG - chr5.2:46859525-46859544 MS.gene042618:CDS 40.0%
TTGAGACCATTAGGGAATGA+TGG + chr5.2:46859311-46859330 None:intergenic 40.0%
!! ATGATGGGTTGTCAATGGAA+AGG + chr5.2:46859295-46859314 None:intergenic 40.0%
!! GAACATCATCATGTTGGTTC+CGG + chr5.2:46860451-46860470 None:intergenic 40.0%
!! TGATGGGTTGTCAATGGAAA+GGG + chr5.2:46859294-46859313 None:intergenic 40.0%
CATATGGGGCTTGCGAATAT+TGG - chr5.2:46859807-46859826 MS.gene042618:intron 45.0%
CCCGTGGATAACTTTCATCT+TGG + chr5.2:46860490-46860509 None:intergenic 45.0%
GACAACCCATCATTCCCTAA+TGG - chr5.2:46859302-46859321 MS.gene042618:CDS 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5.2:46860588-46860607 None:intergenic 45.0%
!! AGGGAATGATGGGTTGTCAA+TGG + chr5.2:46859300-46859319 None:intergenic 45.0%
!!! AATTTTATTAAAATTGTAAT+TGG - chr5.2:46860316-46860335 MS.gene042618:CDS 5.0%
GTTCCGGTGACCATGACAAA+CGG + chr5.2:46860003-46860022 None:intergenic 50.0%
TGTGCATCATCTTGTTCCCG+TGG + chr5.2:46860506-46860525 None:intergenic 50.0%
TTCCGGTGACCATGACAAAC+GGG + chr5.2:46860002-46860021 None:intergenic 50.0%
TTGTTCCTCATACAGCGGCT+TGG + chr5.2:46859834-46859853 None:intergenic 50.0%
CGAAGAAGCCCCGTTTGTCA+TGG - chr5.2:46859990-46860009 MS.gene042618:CDS 55.0%
TCCGGTGACCATGACAAACG+GGG + chr5.2:46860001-46860020 None:intergenic 55.0%
TTGGACCAAGCCGCTGTATG+AGG - chr5.2:46859826-46859845 MS.gene042618:intron 55.0%
GCCCCGTTTGTCATGGTCAC+CGG - chr5.2:46859997-46860016 MS.gene042618:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 46859287 46860640 46859287 ID=MS.gene042618
chr5.2 mRNA 46859287 46860640 46859287 ID=MS.gene042618.t1;Parent=MS.gene042618
chr5.2 exon 46860523 46860640 46860523 ID=MS.gene042618.t1.exon1;Parent=MS.gene042618.t1
chr5.2 CDS 46860523 46860640 46860523 ID=cds.MS.gene042618.t1;Parent=MS.gene042618.t1
chr5.2 exon 46860272 46860421 46860272 ID=MS.gene042618.t1.exon2;Parent=MS.gene042618.t1
chr5.2 CDS 46860272 46860421 46860272 ID=cds.MS.gene042618.t1;Parent=MS.gene042618.t1
chr5.2 exon 46859860 46860168 46859860 ID=MS.gene042618.t1.exon3;Parent=MS.gene042618.t1
chr5.2 CDS 46859860 46860168 46859860 ID=cds.MS.gene042618.t1;Parent=MS.gene042618.t1
chr5.2 exon 46859694 46859770 46859694 ID=MS.gene042618.t1.exon4;Parent=MS.gene042618.t1
chr5.2 CDS 46859694 46859770 46859694 ID=cds.MS.gene042618.t1;Parent=MS.gene042618.t1
chr5.2 exon 46859287 46859574 46859287 ID=MS.gene042618.t1.exon5;Parent=MS.gene042618.t1
chr5.2 CDS 46859287 46859574 46859287 ID=cds.MS.gene042618.t1;Parent=MS.gene042618.t1
Gene Sequence

>MS.gene042618

ATGGCCCTTTCCATTGACAACCCATCATTCCCTAATGGTCTCAACGTTATTGCAATTGATCATGACACCACTTTCCTTTACACCATTGAGGAGATGTGTAATCGATGCCATTATCAAGGTTAGTTACTTGGTAAATTACACCTTACTCATCAATTCTAGAGTTTTCATCTTTTTCCAAAAATTCTTGATTTTACATATTTTTACTTTTGTTTATTTTAGTTACCAAATGCAATACAGCTTCTGATGCCTTGAACTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTAATTGATGTTCATATGCCAAACATGGATGCCTATGACTTTGTTCAACATGCTACAGTACAACTCAATATTCCTGTTATTAGTAAGTTTTTCTTCAACTTTATACTTATACTTTGTTACATTCATTCAAATATATATACTTATGAAGTGTGACTGTGACTGACATTGATATTTCTTACTTGTAGTGATGTCTACTGAATATCCCACATTAAGTTCTGTTACAAAGTCTATCACATATGGGGCTTGCGAATATTGGACCAAGCCGCTGTATGAGGAACAATTCAAGAACATGTGGCAGCATGTTGTAAGAAAAGGTTTGAGCAAAAATAAAAATCTTGAAAATATTGGAAGCTTGGATGTTCAAGGTCATAGAAAACGGGAAAGAGAAGATGATAATGTCACAAAAGAATCAAATGCGAAGAAGCCCCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTATCAATCAACTAGGACTTGATAGTATGGTTCTTTCCTAATTCTTTTCTTTGCTTTGTTTGTATTATTTAACAACTGGACTAATGTGCATTTTTCTCTCACTTATTTTTCAGAGGCAAAACCTAAAATGATTATCAAAAAGATGGATGTTCATGGTTTGAGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTTTAATAGAATCTAAGAAAATTATTGATAATATACCACATGTTTTGTGTGATTTTAAATTTAAATTTGTGGAGTAATTTTATTAAAATTGTAATTGGAATGTTGTTTGCAGAAATATAGAAATTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTTTCAAGCTCATCAGATTGACCGGAACCAACATGATGATGTTCGAGATGAATGCATTGACCAAGATGAAAGTTATCCACGGGAACAAGATGATGCACAACAACTTTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACATACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG
Protein sequence

>MS.gene042618.t1

MALSIDNPSFPNGLNVIAIDHDTTFLYTIEEMCNRCHYQVTKCNTASDALNLLERKDCFDVMLIDVHMPNMDAYDFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKNMWQHVVRKGLSKNKNLENIGSLDVQGHRKREREDDNVTKESNAKKPRLSWSPELHQRFLWAINQLGLDKAKPKMIIKKMDVHGLSVGHVASHLQKYRNYLKRSIDEKKCGKKKSKLSDDFQAHQIDRNQHDDVRDECIDQDESYPREQDDAQQLWMEIGKSDNIRNFFSDIPNLFPDCVVINSSVDYFG